##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938629_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2132216 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.637096335455695 31.0 31.0 34.0 30.0 34.0 2 31.88784250751331 31.0 31.0 34.0 30.0 34.0 3 31.951976253812933 31.0 31.0 34.0 30.0 34.0 4 35.653289347795905 37.0 35.0 37.0 33.0 37.0 5 35.50917918259689 37.0 35.0 37.0 33.0 37.0 6 35.557145242320665 37.0 35.0 37.0 33.0 37.0 7 35.47895006884856 37.0 35.0 37.0 33.0 37.0 8 35.463672535990725 37.0 35.0 37.0 33.0 37.0 9 37.01206631973496 39.0 37.0 39.0 33.0 39.0 10-11 36.93698433929771 39.0 37.0 39.0 33.0 39.0 12-13 36.80969845456558 39.0 37.0 39.0 32.0 39.0 14-15 37.80641923707542 40.0 37.0 41.0 32.0 41.0 16-17 37.70209983416314 39.0 37.0 41.0 32.0 41.0 18-19 37.6851308685424 39.0 37.0 41.0 32.0 41.0 20-21 37.66785588326886 39.0 36.5 41.0 32.0 41.0 22-23 37.69821139134122 39.0 37.0 41.0 32.0 41.0 24-25 37.60783124223812 39.0 37.0 41.0 32.0 41.0 26-27 37.408549368356674 39.0 36.0 41.0 32.0 41.0 28-29 37.25477390658357 39.0 36.0 41.0 31.0 41.0 30-31 36.987008351874294 39.0 36.0 40.0 31.0 41.0 32-33 36.75096284804167 39.0 35.5 40.0 30.0 41.0 34-35 36.55948482705317 38.5 35.0 40.0 30.0 41.0 36-37 36.559008327486524 39.0 35.0 40.0 30.0 41.0 38-39 36.38791356035223 38.5 35.0 40.0 30.0 41.0 40-41 36.1842177340382 38.0 35.0 40.0 30.0 41.0 42-43 35.943085269034654 38.0 35.0 40.0 29.0 41.0 44-45 35.64512413376506 38.0 34.5 40.0 28.0 41.0 46-47 35.25977715203338 38.0 34.0 40.0 27.0 41.0 48-49 34.831578742491374 38.0 33.5 40.0 26.0 41.0 50-51 34.41160862689333 38.0 33.0 40.0 24.0 41.0 52-53 33.82190383150675 37.0 32.5 40.0 23.0 41.0 54-55 33.44296262667572 37.0 32.0 39.5 22.5 41.0 56-57 33.0184500538407 36.0 31.0 39.0 21.5 40.5 58-59 32.48697247370811 36.0 31.0 39.0 19.5 40.0 60-61 32.01097801536054 35.5 30.0 39.0 18.0 40.0 62-63 31.45789755822112 35.0 29.5 38.0 13.5 40.0 64-65 30.89100775906381 34.5 29.0 38.0 9.5 40.0 66-67 30.32572075249412 34.0 28.0 37.5 8.0 40.0 68-69 29.686960420520247 34.0 28.0 37.0 6.5 39.0 70-71 29.056414547119054 33.5 26.5 36.0 2.0 39.0 72-73 28.40043902681529 33.0 26.0 36.0 2.0 39.0 74-75 27.715306516788168 32.0 25.5 35.0 2.0 38.0 76-77 26.526527096691893 30.5 24.0 34.0 2.0 36.0 78-79 27.10223706228637 32.0 25.0 35.0 2.0 37.0 80-81 26.73420047499878 32.0 24.5 35.0 2.0 36.0 82-83 26.16033882120761 32.0 23.5 35.0 2.0 36.0 84-85 25.48241946406931 31.0 20.5 34.0 2.0 35.5 86-87 24.737117862355408 31.0 18.5 34.0 2.0 35.0 88-89 23.871518645390523 30.5 11.5 34.0 2.0 35.0 90-91 23.223466571867014 30.0 4.5 34.0 2.0 35.0 92-93 22.332046565638755 29.0 2.0 34.0 2.0 35.0 94-95 21.42624949817467 29.0 2.0 34.0 2.0 35.0 96-97 20.338845126384946 28.0 2.0 34.0 2.0 35.0 98-99 19.069336314894926 26.5 2.0 33.0 2.0 35.0 100-101 16.168894474105812 13.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32353365303799464 1101 2 0.3249123898108728 1101 3 0.21256636145065855 1101 4 0.2515960127703778 1101 5 0.19687698426140798 1101 6 0.19688305749379964 1101 7 0.25073844970017944 1101 8 0.2757422968280139 1101 9 0.3845918306363174 1101 10-11 0.4396486804648063 1101 12-13 0.444797362650192 1101 14-15 0.5352551113547293 1101 16-17 0.5730042118465093 1101 18-19 0.628521006439577 1101 20-21 0.5557396875460299 1101 22-23 0.6130352027556754 1101 24-25 0.6372072374702284 1101 26-27 0.5105658794721393 1101 28-29 0.44185099638713154 1101 30-31 0.5288249137734624 1101 32-33 0.6575413924645801 1101 34-35 0.4200196994576757 1101 36-37 0.6134027401514075 1101 38-39 0.7991646124944154 1101 40-41 0.9759108801721084 1101 42-43 0.8253141419217016 1101 44-45 0.7903121401003332 1101 46-47 0.9460076225060021 1101 48-49 0.9552771861190976 1101 50-51 1.087523370912649 1101 52-53 1.1996412706306927 1101 54-55 1.3401864532898315 1101 56-57 1.3191576655631891 1101 58-59 1.3306164195342944 1101 60-61 1.4616265768153163 1101 62-63 1.6654419514125038 1101 64-65 1.6666099267118355 1101 66-67 1.672526708789281 1101 68-69 1.9095853404592056 1101 70-71 2.14304360164299 1101 72-73 2.1873222082183013 1101 74-75 2.2338026559725925 1101 76-77 2.0706363682384037 1101 78-79 2.4002073077908044 1101 80-81 2.678743814746227 1101 82-83 2.8430643385339778 1101 84-85 2.9048716332086038 1101 86-87 3.0499453626301936 1101 88-89 3.41647437816842 1101 90-91 3.6171781312898794 1101 92-93 3.696675170098498 1101 94-95 3.800587737955947 1101 96-97 4.079485061700829 1101 98-99 4.2627205963256465 1101 100-101 4.505626984004973 1102 1 0.36735895293273657 1102 2 0.3789571630739168 1102 3 0.34681612440550325 1102 4 0.35514581834986103 1102 5 0.2431973475276763 1102 6 0.23991784441412278 1102 7 0.34852784051587804 1102 8 0.37074412359802267 1102 9 0.5247916444797553 1102 10-11 0.5066252456152611 1102 12-13 0.5215308369057823 1102 14-15 0.5475179574624036 1102 16-17 0.4851378792177883 1102 18-19 0.5195483036028676 1102 20-21 0.7230036427640698 1102 22-23 0.699693474979199 1102 24-25 0.590749105299075 1102 26-27 0.5974116130942733 1102 28-29 0.6201224370127605 1102 30-31 0.5413970059470614 1102 32-33 0.4347480871417204 1102 34-35 0.5463995632328249 1102 36-37 0.7032379611470816 1102 38-39 0.7490469598068898 1102 40-41 0.8503887284110192 1102 42-43 0.8375230548195702 1102 44-45 0.9335396077621567 1102 46-47 0.9430256379378577 1102 48-49 1.0294404602128822 1102 50-51 1.199956171873012 1102 52-53 1.3719764698791153 1102 54-55 1.304912285557549 1102 56-57 1.5467484168875885 1102 58-59 1.6554735400228182 1102 60-61 1.651944587027824 1102 62-63 1.7943187270764582 1102 64-65 1.767214718068601 1102 66-67 1.6558450365118205 1102 68-69 2.032175200145872 1102 70-71 2.134296091704492 1102 72-73 2.211500922868126 1102 74-75 2.2669437435096746 1102 76-77 2.376234330954901 1102 78-79 2.7978226117740306 1102 80-81 3.0292387824298004 1102 82-83 2.9109426539040726 1102 84-85 3.0183122022909536 1102 86-87 3.2933295193148666 1102 88-89 3.3319390736632535 1102 90-91 3.723360007991605 1102 92-93 3.6753912334961143 1102 94-95 3.869850810235146 1102 96-97 4.149341181815306 1102 98-99 4.5992742642188595 1102 100-101 4.983831116854649 1103 1 0.30885114050784424 1103 2 0.3388927007980591 1103 3 0.33645062221460975 1103 4 0.2761076363014041 1103 5 0.22108112733604202 1103 6 0.24296749273901241 1103 7 0.3314279072687327 1103 8 0.2679996908990674 1103 9 0.42666661809419537 1103 10-11 0.5037401804904604 1103 12-13 0.5387794697680022 1103 14-15 0.6823949871651251 1103 16-17 0.6317257089641473 1103 18-19 0.6253097814784923 1103 20-21 0.6151640276818497 1103 22-23 0.4790750768554659 1103 24-25 0.5364475169823706 1103 26-27 0.5491728518801509 1103 28-29 0.5789787269333218 1103 30-31 0.6601006796948781 1103 32-33 0.6855815573664756 1103 34-35 0.7096115501564526 1103 36-37 0.8004836430064017 1103 38-39 0.8619928339829741 1103 40-41 0.7006444541299288 1103 42-43 0.8545098175234074 1103 44-45 0.9892250534241995 1103 46-47 1.04268050435482 1103 48-49 1.0932818132937 1103 50-51 1.3037614608560233 1103 52-53 1.4363877510699794 1103 54-55 1.2997463585225617 1103 56-57 1.3704594326135933 1103 58-59 1.6662354003584596 1103 60-61 1.4854417720803745 1103 62-63 1.6459071064784538 1103 64-65 1.6387241435133575 1103 66-67 1.943067173046277 1103 68-69 1.9176540296259716 1103 70-71 2.1115366908165996 1103 72-73 2.0666878747538213 1103 74-75 2.1722954457911 1103 76-77 2.0528824723455585 1103 78-79 2.5696318189866822 1103 80-81 2.8027999908696657 1103 82-83 2.8681222851290293 1103 84-85 3.036104847940294 1103 86-87 3.251787465642561 1103 88-89 3.534420716627853 1103 90-91 3.787210974821246 1103 92-93 4.03057734940365 1103 94-95 3.8888619985488404 1103 96-97 4.298179360269057 1103 98-99 4.5313173906601385 1103 100-101 4.7637803479010365 1104 1 0.30713353787772846 1104 2 0.29831641875114556 1104 3 0.2579090466876117 1104 4 0.157936294662683 1104 5 0.08083757942301162 1104 6 0.06840717856228906 1104 7 0.07369769298041007 1104 8 0.19859848001627967 1104 9 0.16061805045890054 1104 10-11 0.2799791820795505 1104 12-13 0.36597475118256284 1104 14-15 0.439386694474571 1104 16-17 0.48846447738271337 1104 18-19 0.4873155371614999 1104 20-21 0.513561927663531 1104 22-23 0.3882079431527288 1104 24-25 0.4269359641148114 1104 26-27 0.43651469991392133 1104 28-29 0.4615738607066433 1104 30-31 0.545116336002728 1104 32-33 0.5364449499153636 1104 34-35 0.43733755013320064 1104 36-37 0.6799907209246498 1104 38-39 0.721052781863456 1104 40-41 0.7733969649734576 1104 42-43 0.7567228523770879 1104 44-45 0.7299281056523981 1104 46-47 0.7525951234448058 1104 48-49 0.9044984982785138 1104 50-51 1.0543495999146728 1104 52-53 1.180610515329569 1104 54-55 1.141657145836966 1104 56-57 1.3166696196993612 1104 58-59 1.7229949796971837 1104 60-61 1.643677743856113 1104 62-63 1.752912326851657 1104 64-65 1.690898102756833 1104 66-67 1.8955656463106614 1104 68-69 2.0848151394294376 1104 70-71 2.0697579666342705 1104 72-73 2.3683605073512624 1104 74-75 2.4541359531444904 1104 76-77 2.4818930754893316 1104 78-79 2.7941614982057956 1104 80-81 2.943021184085225 1104 82-83 3.2705720993344265 1104 84-85 3.199809901465258 1104 86-87 3.4047363008525515 1104 88-89 3.5311938210597766 1104 90-91 3.868466591063065 1104 92-93 4.198870829891707 1104 94-95 4.357787966607486 1104 96-97 4.727762664698332 1104 98-99 4.826511872518349 1104 100-101 4.883974037821535 1105 1 0.23859339732251073 1105 2 0.20311794826663387 1105 3 0.2187011299726258 1105 4 0.24840640545853887 1105 5 0.14433391617281188 1105 6 0.2423579044042512 1105 7 0.09263133258978229 1105 8 0.20189160782145876 1105 9 0.2912328926096208 1105 10-11 0.30600730361178563 1105 12-13 0.40630041615488466 1105 14-15 0.4325098573379407 1105 16-17 0.4751052053749021 1105 18-19 0.4110777055057113 1105 20-21 0.33255304258948826 1105 22-23 0.27812892090517494 1105 24-25 0.30712265497587765 1105 26-27 0.3856193261621783 1105 28-29 0.4768577497285875 1105 30-31 0.5181790788864475 1105 32-33 0.5748693065521309 1105 34-35 0.6082867008912061 1105 36-37 0.6871379387327892 1105 38-39 0.7759805570092979 1105 40-41 0.620287791202017 1105 42-43 0.8751562877441614 1105 44-45 0.8356921087780833 1105 46-47 0.9186760624426924 1105 48-49 0.9839986708598616 1105 50-51 1.2204953176151108 1105 52-53 1.4070375260478727 1105 54-55 1.393640083107286 1105 56-57 1.3229772394811974 1105 58-59 1.5369438447296417 1105 60-61 1.5335934771558861 1105 62-63 1.8938062962555264 1105 64-65 1.9842069386689758 1105 66-67 1.7690226360686374 1105 68-69 1.906008244644621 1105 70-71 1.7451712149375425 1105 72-73 2.124741209552397 1105 74-75 2.3137067005197913 1105 76-77 2.2368566100328877 1105 78-79 2.5044541444925166 1105 80-81 2.8213598852129955 1105 82-83 2.929508248511521 1105 84-85 3.12753085462705 1105 86-87 3.4207101267604223 1105 88-89 3.4821348798215404 1105 90-91 3.736646398871354 1105 92-93 3.92376073037725 1105 94-95 3.990663847699814 1105 96-97 4.1145680490807806 1105 98-99 4.623059837796962 1105 100-101 4.736805566383307 1106 1 0.19215792582806657 1106 2 0.14346966357471658 1106 3 0.0685713052297352 1106 4 0.062270289305736526 1106 5 0.11942070008566219 1106 6 0.15057840751169493 1106 7 0.129130847599491 1106 8 -0.02501778839808111 1106 9 0.11254956715119846 1106 10-11 0.21945305163109197 1106 12-13 0.1152008549224135 1106 14-15 0.08412777991345877 1106 16-17 0.1514134635381339 1106 18-19 0.312206327410685 1106 20-21 0.46658510289837807 1106 22-23 0.1368046678454391 1106 24-25 0.11523852726390515 1106 26-27 0.1325796371603829 1106 28-29 0.29628957783880594 1106 30-31 0.3529566313621615 1106 32-33 0.4154963824355349 1106 34-35 0.4563469043914665 1106 36-37 0.37699241064936473 1106 38-39 0.4724704942598592 1106 40-41 0.49078840928131484 1106 42-43 0.5434188601512631 1106 44-45 0.20119927379044356 1106 46-47 -0.02285156301888236 1106 48-49 0.5759566852493592 1106 50-51 0.515004727705346 1106 52-53 0.6592288937049915 1106 54-55 0.7746685841085608 1106 56-57 0.7758991824877199 1106 58-59 1.2193880485677582 1106 60-61 0.9157065470194716 1106 62-63 1.1829104910002002 1106 64-65 0.7579797446908785 1106 66-67 0.8224836379867035 1106 68-69 0.894892890958161 1106 70-71 0.8881163642500418 1106 72-73 1.4422101200958544 1106 74-75 1.789227912524769 1106 76-77 1.7351824394321547 1106 78-79 2.1378706439261634 1106 80-81 1.9353255327510723 1106 82-83 2.219193230511575 1106 84-85 2.360388961403956 1106 86-87 2.5127599667533396 1106 88-89 2.782124476794312 1106 90-91 2.7977804566970796 1106 92-93 2.571181312369472 1106 94-95 3.0653406322306473 1106 96-97 3.474744776559632 1106 98-99 3.9907553192992395 1106 100-101 4.3215397729122635 1107 1 0.23784593979756252 1107 2 0.2434668765951038 1107 3 0.16729361923007602 1107 4 0.13587788065380124 1107 5 0.0080641425628869 1107 6 -0.02661141366992581 1107 7 0.10686230385400108 1107 8 0.15924519484256194 1107 9 0.1725989831828727 1107 10-11 0.161952088201069 1107 12-13 0.19443151400464842 1107 14-15 0.42358083996902707 1107 16-17 0.3543890928429363 1107 18-19 0.3178117122397168 1107 20-21 0.36099259583723153 1107 22-23 0.31916933355684307 1107 24-25 0.4107402460615006 1107 26-27 0.43566453528666926 1107 28-29 0.44532244786080355 1107 30-31 0.2737811352557671 1107 32-33 0.4788942103212861 1107 34-35 0.49530436258917376 1107 36-37 0.5508328589494482 1107 38-39 0.614500453329704 1107 40-41 0.7964251236053315 1107 42-43 0.7336258942956064 1107 44-45 0.8484204148567756 1107 46-47 0.753838427784089 1107 48-49 0.7108640854490602 1107 50-51 0.6250377582189373 1107 52-53 0.7861259378728533 1107 54-55 0.9814723701723054 1107 56-57 1.0282524984649015 1107 58-59 1.013553074280047 1107 60-61 1.1745736109748925 1107 62-63 1.2615106199700818 1107 64-65 1.2833105063147485 1107 66-67 1.5573655709588827 1107 68-69 1.8228088164324028 1107 70-71 1.693536366883258 1107 72-73 1.8600947449059326 1107 74-75 1.7707821437844906 1107 76-77 1.7041906494896892 1107 78-79 2.0518531128633555 1107 80-81 2.315195082884511 1107 82-83 2.3367899314965292 1107 84-85 2.8538485929652495 1107 86-87 3.0722924461176753 1107 88-89 3.14769586047203 1107 90-91 3.156095794791341 1107 92-93 3.2531879953543843 1107 94-95 3.3881252477187616 1107 96-97 3.856948374069802 1107 98-99 4.153422285806471 1107 100-101 4.272332188099188 1108 1 0.17499716875852656 1108 2 0.23630187826770666 1108 3 0.19362826695509838 1108 4 0.1462468864069919 1108 5 0.024565396778747584 1108 6 0.007273547206310127 1108 7 -0.014649085487391744 1108 8 0.0472539056489083 1108 9 0.11009553610301026 1108 10-11 0.09677749775206479 1108 12-13 0.18008464916525213 1108 14-15 0.2898916637524067 1108 16-17 0.23925491970143753 1108 18-19 0.29738092912950975 1108 20-21 0.24352672785067142 1108 22-23 0.33221355757770255 1108 24-25 0.24218263694661601 1108 26-27 0.28544975022808927 1108 28-29 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90-91 2.5023010060189357 2316 92-93 2.3232433433943918 2316 94-95 2.9429927809872503 2316 96-97 2.9999493973907434 2316 98-99 3.2849624313524934 2316 100-101 3.577997204146005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 40.0 5 77.0 6 170.0 7 469.0 8 1169.0 9 2013.0 10 3289.0 11 4986.0 12 7243.0 13 10057.0 14 13287.0 15 17458.0 16 21206.0 17 25647.0 18 26195.0 19 24888.0 20 25800.0 21 27805.0 22 30678.0 23 33887.0 24 38736.0 25 44566.0 26 51883.0 27 60658.0 28 71046.0 29 82628.0 30 95713.0 31 110232.0 32 128245.0 33 151286.0 34 179895.0 35 216920.0 36 253665.0 37 251100.0 38 114497.0 39 4767.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.79256866337056 19.426051981649124 15.182434783750598 23.598944571229715 2 16.22214319026568 20.414329724033546 38.98817930077988 24.37534778492089 3 19.72830081075466 23.532989933923048 29.9475989946586 26.791110260663697 4 13.876877389532769 16.884827803562118 35.6572223452033 33.581072461701815 5 14.005045729860974 36.977059486845334 34.332417265146816 14.685477518146882 6 33.40102503686306 35.91728042562292 16.508036709226456 14.173657828287567 7 29.083451207569965 32.06011961264713 20.237490010392946 18.618939169389968 8 26.41782070859613 35.8499326522266 18.95661602764448 18.77563061153279 9 25.75892873892701 16.844353480135222 19.692845377766606 37.70387240317116 10-11 25.29469340817253 26.402414201938267 27.094253115069016 21.208639274820186 12-13 26.00311131705231 23.376712303068732 27.539072026473864 23.081104353405095 14-15 22.506444000045022 25.147053581813473 25.505999392181657 26.840503025959844 16-17 22.532121511141458 28.26657805775775 26.406072367902688 22.7952280631981 18-19 22.540680681506938 26.866719882038222 28.400617010659335 22.191982425795512 20-21 22.853875967538 26.058218304336894 28.823533825841285 22.264371902283823 22-23 22.62242662094272 25.997530268978373 28.7067539123616 22.673289197717306 24-25 22.61547148874422 26.202177757829613 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22.846536185827325 50-51 22.924975706026032 26.917559009030978 27.536211153091433 22.621254131851558 52-53 23.126807978178572 26.70085957520251 27.489217790317678 22.683114656301235 54-55 22.806043760586114 26.80563948625268 27.608091208416784 22.78022554474442 56-57 22.814878980365965 26.991566520465092 27.4286704536501 22.76488404551884 58-59 22.83633553073422 26.752144248049913 27.45258923110979 22.95893099010607 60-61 22.609834088103646 26.878421323168013 27.628790891729544 22.882953696998804 62-63 22.647869634220925 26.856988222581577 27.69855399265365 22.796588150543847 64-65 22.452579851197065 26.880766301350334 27.549085083312384 23.117568764140216 66-67 22.416144556050035 26.89733802142471 27.502836257762482 23.183681164762774 68-69 22.772937074399618 26.634402780028317 27.571248308971658 23.021411836600407 70-71 22.608544350103365 26.6791450772342 27.356210627816317 23.35609994484611 72-73 22.60870849857613 26.804320012606603 27.310131806533672 23.276839682283594 74-75 22.569758410967744 27.08862516743144 27.05410708858765 23.287509333013166 76-77 22.59013627137213 26.866274336183576 27.33827154472155 23.205317847722746 78-79 22.65980557316895 26.96720219715076 27.338763990139835 23.034228239540457 80-81 22.730306277744035 26.914288662259665 27.217822780535734 23.13758227946056 82-83 22.71315789843949 27.029242744656074 27.22287022667336 23.03472913023107 84-85 22.60388127345242 27.063267622980902 27.145322508060676 23.187528595506 86-87 22.73751830870052 26.984550575630706 27.023735767520897 23.254195348147885 88-89 22.606908166646384 27.13053628960112 26.793820793442315 23.468734750310183 90-91 22.59425224903685 27.044909917758286 27.0071084173764 23.353729415828465 92-93 22.57677895946855 26.842848623856437 27.05286560859316 23.527506808081856 94-95 22.349963605938612 26.738261977210563 27.17935237330552 23.732422043545306 96-97 22.549521249254298 26.467604595406847 27.142583115406694 23.840291039932165 98-99 22.333712907135112 26.6920659070188 26.896548004517367 24.07767318132872 100-101 22.457154541932788 26.40950793500416 27.183451067865178 23.949886455197873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 200.5 2 174.0 3 231.0 4 205.5 5 218.5 6 387.0 7 853.0 8 1365.5 9 1588.0 10 1447.0 11 1218.0 12 1261.5 13 1378.5 14 1487.5 15 1609.5 16 1751.5 17 1995.0 18 2270.0 19 2545.5 20 2956.5 21 3576.0 22 4412.0 23 5401.5 24 6843.5 25 8822.5 26 11402.5 27 14334.0 28 16957.0 29 19860.0 30 23173.5 31 27812.0 32 33811.5 33 36701.5 34 37687.0 35 40460.5 36 45027.0 37 49903.0 38 53457.0 39 56329.0 40 59647.0 41 63856.5 42 66744.5 43 68684.0 44 71811.0 45 76074.0 46 78468.5 47 80086.0 48 80596.0 49 80515.0 50 80497.0 51 78143.5 52 76370.5 53 76720.5 54 77310.5 55 73370.5 56 66903.5 57 60814.5 58 55222.0 59 48710.5 60 40344.5 61 33527.0 62 28094.5 63 21910.0 64 16118.0 65 12158.0 66 9197.5 67 7153.5 68 5562.5 69 4188.0 70 3244.0 71 2357.5 72 1722.5 73 1373.0 74 1051.0 75 764.0 76 586.5 77 409.0 78 242.5 79 161.5 80 103.0 81 63.5 82 37.5 83 20.5 84 13.0 85 7.0 86 3.5 87 2.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.379912729291967E-5 2 0.04080262037242006 3 2.3449781823229918E-4 4 0.0 5 0.09839528453027273 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010786899638685762 26-27 0.01139659396608974 28-29 0.0 30-31 0.0 32-33 4.6899563646459836E-4 34-35 0.014773362548634848 36-37 0.0031891703279592685 38-39 0.011982838511670488 40-41 0.007527379965256803 42-43 0.0054403493829893405 44-45 0.0026967249096714403 46-47 9.379912729291967E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.3449781823229917E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3449781823229918E-4 68-69 3.517467273484488E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.6899563646459834E-5 82-83 0.0013600873457473351 84-85 0.004994803528347972 86-87 0.0015476856003331743 88-89 2.3449781823229917E-5 90-91 0.0011959388729847256 92-93 3.517467273484488E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.44192145636277E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2132216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.15636605706703 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52421470325685 47.237314040328435 2 12.674593507166648 15.249149732832839 3 3.842167155839561 6.933924416373744 4 1.6641671547618457 4.004409941680052 5 0.9002740495548313 2.707860763834927 6 0.5410255536592623 1.9527678751292368 7 0.36424869320299846 1.5338314406889515 8 0.23978827629280047 1.1539833059890268 9 0.18214858353711255 0.9861657181231318 >10 0.9831444513761821 10.592495067875173 >50 0.05608541689091745 2.2854919161237666 >100 0.02409146452379364 2.646742879026492 >500 0.002726627842375336 1.1493397582725693 >1k 0.0012464584422286432 1.3172534933954663 >5k 7.79036526392902E-5 0.24926965032625892 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5319 0.24945877903551983 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4561 0.21390890979150332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3193 0.14975030672314624 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.6899563646459834E-5 0.0 0.0 0.0 0.0 9 4.6899563646459834E-5 0.0 0.0 0.0 0.0 10-11 4.6899563646459834E-5 0.0 0.0 0.0 0.0 12-13 4.6899563646459834E-5 0.0 0.0 0.0 0.0 14-15 4.6899563646459834E-5 0.0 0.0 2.3449781823229917E-5 0.0 16-17 4.6899563646459834E-5 0.0 0.0 4.6899563646459834E-5 0.0 18-19 4.6899563646459834E-5 0.0 0.0 7.034934546968975E-5 0.0 20-21 4.6899563646459834E-5 0.0 0.0 1.1724890911614958E-4 0.0 22-23 4.6899563646459834E-5 0.0 0.0 2.81397381878759E-4 0.0 24-25 9.379912729291967E-5 0.0 0.0 4.924454182878282E-4 0.0 26-27 9.379912729291967E-5 0.0 0.0 8.910917092827368E-4 0.0 28-29 1.406986909393795E-4 0.0 0.0 0.002016681236797773 0.0 30-31 1.406986909393795E-4 0.0 0.0 0.00804327516536786 0.0 32-33 1.406986909393795E-4 0.0 0.0 0.02016681236797773 0.0 34-35 1.406986909393795E-4 0.0 0.0 0.03876248935379906 0.0 36-37 1.406986909393795E-4 0.0 0.0 0.07396061187046715 0.0 38-39 1.406986909393795E-4 0.0 0.0 0.1257377301361588 0.0 40-41 1.406986909393795E-4 0.0 0.0 0.18419803622147102 0.0 42-43 1.406986909393795E-4 0.0 0.0 0.23977401914252588 0.0 44-45 1.406986909393795E-4 0.0 0.0 0.3151416179223868 0.0 46-47 1.406986909393795E-4 0.0 0.0 0.40338314692320104 0.0 48-49 1.406986909393795E-4 0.0 0.0 0.5013797851624788 0.0 50-51 1.406986909393795E-4 0.0 0.0 0.6070914016215994 0.0 52-53 1.406986909393795E-4 0.0 0.0 0.7120291752805532 0.0 54-55 1.406986909393795E-4 0.0 0.0 0.8262296127596829 0.0 56-57 1.406986909393795E-4 0.0 0.0 0.9643019281348606 0.0 58-59 1.406986909393795E-4 0.0 0.0 1.1167489597676783 0.0 60-61 1.406986909393795E-4 0.0 0.0 1.281014681439404 0.0 62-63 1.406986909393795E-4 0.0 0.0 1.44593699700218 0.0 64-65 1.406986909393795E-4 0.0 0.0 1.6210130680944146 0.0 66-67 1.406986909393795E-4 0.0 0.0 1.8205003620646314 0.0 68-69 1.406986909393795E-4 0.0 0.0 2.045641717349462 0.0 70-71 1.406986909393795E-4 0.0 0.0 2.2878310640197803 0.0 72-73 1.406986909393795E-4 0.0 0.0 2.535531109418558 0.0 74-75 1.406986909393795E-4 0.0 0.0 2.790406788055244 0.0 76-77 1.406986909393795E-4 0.0 0.0 3.0638781436777514 0.0 78-79 1.406986909393795E-4 0.0 0.0 3.374001508289967 0.0 80-81 1.8759825458583934E-4 0.0 0.0 3.7083015979619325 0.0 82-83 1.8759825458583934E-4 0.0 0.0 4.042296840470196 0.0 84-85 1.8759825458583934E-4 0.0 0.0 4.3863285895988025 0.0 86-87 1.8759825458583934E-4 0.0 0.0 4.749213963313285 0.0 88-89 1.8759825458583934E-4 0.0 0.0 5.145843573071396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 52.9562 1 GTATCAA 4905 0.0 41.59902 1 ATCAACG 6460 0.0 31.129255 3 TATCAAC 6640 0.0 30.729393 2 TCAACGC 6590 0.0 30.58731 4 CAACGCA 6790 0.0 29.616344 5 AACGCAG 7080 0.0 28.375937 6 TGGTATC 1455 0.0 26.15121 2 ACGCAGA 8260 0.0 24.322231 7 CGCAGAG 8340 0.0 24.031977 8 TACATGG 5645 0.0 23.254656 2 GTAAGGT 1070 0.0 23.103642 4 TAAGGTG 1235 0.0 23.096445 5 GTACATG 5955 0.0 23.002525 1 ACATGGG 5875 0.0 22.091015 3 GTGGTAT 1835 0.0 22.031681 1 GCAGAGT 9505 0.0 20.83661 9 AGAGTAC 8560 0.0 20.113024 10-11 GAGTACT 6000 0.0 19.868525 12-13 CATGGGG 4175 0.0 19.24381 4 >>END_MODULE