FastQCFastQC Report
Thu 26 May 2016
SRR938628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118352
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.24046995022545828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40580.19156400824792102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.12703271222157603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.054.6815261
GTATCAA50200.045.225121
ATCAACG65050.034.555553
TATCAAC65900.034.109842
TCAACGC66100.033.862844
CAACGCA68600.032.490225
AACGCAG71650.031.1683486
ACGCAGA83550.026.665887
CGCAGAG83050.026.5976268
TGGTATC12300.025.5001532
GTGGTAT13950.024.616981
GCAGAGT92250.023.3780759
GTACATG58450.022.3584941
TACATGG55550.022.242992
GGACCGA4300.020.9950666
CAGAGTA85350.020.8152810-11
GAGTACT59600.020.80289612-13
AGAGTAC83250.020.57004410-11
ACATGGG58950.019.9927163
GTGTAGC12350.019.309881
Produced by FastQC (version 0.11.5)