##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2118352 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20638401927536 31.0 31.0 34.0 28.0 34.0 2 31.38540289810192 31.0 31.0 34.0 28.0 34.0 3 31.44791375559869 31.0 31.0 34.0 28.0 34.0 4 35.12646198554348 37.0 35.0 37.0 32.0 37.0 5 35.110032704668534 37.0 35.0 37.0 32.0 37.0 6 35.08818175638421 37.0 35.0 37.0 32.0 37.0 7 35.0014903094481 37.0 35.0 37.0 32.0 37.0 8 34.958163704615664 37.0 35.0 37.0 32.0 37.0 9 36.459036080877965 39.0 37.0 39.0 32.0 39.0 10-11 36.28602706254674 39.0 35.0 39.0 32.0 39.0 12-13 36.0962328262725 38.0 35.0 39.0 31.5 39.0 14-15 37.28082042077993 39.0 37.0 41.0 32.0 41.0 16-17 37.19828999146506 39.0 36.0 41.0 31.0 41.0 18-19 37.17618153168123 39.0 36.0 41.0 31.0 41.0 20-21 37.09933452986095 39.0 36.0 41.0 31.0 41.0 22-23 36.92864217089512 39.0 36.0 41.0 31.0 41.0 24-25 36.782240392531556 39.0 36.0 41.0 30.0 41.0 26-27 36.553404722161375 39.0 36.0 41.0 30.0 41.0 28-29 36.31060890730153 39.0 35.5 40.0 29.5 41.0 30-31 36.03181199347417 38.5 35.0 40.0 29.0 41.0 32-33 35.71511958352531 38.0 35.0 40.0 27.5 41.0 34-35 35.399799938820365 38.0 34.0 40.0 27.0 41.0 36-37 35.1612114039593 38.0 34.0 40.0 25.5 41.0 38-39 34.8819506861938 38.0 34.0 40.0 25.0 41.0 40-41 34.57538336404903 38.0 33.0 40.0 24.0 41.0 42-43 34.13859075356692 38.0 33.0 40.0 23.5 41.0 44-45 33.650774753204374 38.0 32.5 40.0 22.0 41.0 46-47 33.14445474595345 37.0 31.5 40.0 18.5 41.0 48-49 32.62374359879755 37.0 31.0 39.5 15.5 41.0 50-51 31.90153052939266 36.0 30.5 38.5 13.5 40.0 52-53 31.89372210095395 36.0 30.5 39.0 11.0 40.0 54-55 31.821071993700762 36.0 30.5 39.0 8.5 40.0 56-57 31.341649074374796 36.0 29.5 39.0 6.5 40.0 58-59 30.83590994320113 35.0 29.0 39.0 2.0 40.0 60-61 30.23437228562581 35.0 28.0 38.5 2.0 40.0 62-63 29.455249410862784 34.0 26.5 38.0 2.0 40.0 64-65 28.742561670581658 34.0 26.0 38.0 2.0 40.0 66-67 27.989184753053316 33.5 25.5 37.0 2.0 39.0 68-69 27.084058031904046 32.5 22.5 36.5 2.0 39.0 70-71 26.174939056398557 32.0 20.0 36.0 2.0 39.0 72-73 25.212074527746097 31.0 15.5 35.0 2.0 38.0 74-75 24.21537402660181 30.5 7.5 35.0 2.0 37.5 76-77 23.208487777291026 29.5 2.0 34.0 2.0 36.5 78-79 22.1703451551017 29.0 2.0 34.0 2.0 36.0 80-81 21.152129816007914 28.0 2.0 34.0 2.0 35.5 82-83 20.09723714472382 26.0 2.0 33.0 2.0 35.0 84-85 19.069389789798862 25.5 2.0 32.5 2.0 35.0 86-87 18.01970918902996 23.0 2.0 32.0 2.0 34.5 88-89 16.892407635747034 19.0 2.0 31.5 2.0 34.0 90-91 15.697286381111354 9.0 2.0 31.0 2.0 34.0 92-93 14.519586216077403 2.0 2.0 30.5 2.0 34.0 94-95 13.261893443582558 2.0 2.0 29.5 2.0 34.0 96-97 11.874521562044457 2.0 2.0 29.0 2.0 34.0 98-99 10.247137397373052 2.0 2.0 25.0 2.0 33.0 100-101 7.234916576659591 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45562794834729914 1101 2 0.3632345994420447 1101 3 0.3171201039413454 1101 4 0.15863745619862613 1101 5 0.19408165761621632 1101 6 0.3342672746255104 1101 7 0.08266287306086184 1101 8 0.14666742809219357 1101 9 0.2765573958404133 1101 10-11 0.34096450246275367 1101 12-13 0.4011288520012073 1101 14-15 0.4067043989283263 1101 16-17 0.36313708382770926 1101 18-19 0.3616452364388749 1101 20-21 0.684390771795762 1101 22-23 0.7930251132626722 1101 24-25 0.6667525430144039 1101 26-27 0.6769133272227918 1101 28-29 0.6883459267733869 1101 30-31 0.6706822428434194 1101 32-33 0.6968631924780979 1101 34-35 0.7248208604488084 1101 36-37 0.6785728817459926 1101 38-39 0.8314380443652993 1101 40-41 0.8206843962859764 1101 42-43 1.0342124326944884 1101 44-45 1.0534879664138046 1101 46-47 1.0547714063554068 1101 48-49 0.9986162332201474 1101 50-51 0.9569626807633931 1101 52-53 0.9868197106668646 1101 54-55 1.0767597655635583 1101 56-57 1.257691402613606 1101 58-59 1.2999988204461452 1101 60-61 1.3656872118371837 1101 62-63 1.2654906445433909 1101 64-65 1.4971723425811732 1101 66-67 1.5773451821954723 1101 68-69 1.6444693577422456 1101 70-71 1.9479453180742112 1101 72-73 2.259266239978011 1101 74-75 2.2995633485577436 1101 76-77 2.5668030755831275 1101 78-79 2.4039067229396878 1101 80-81 2.7320649420318155 1101 82-83 2.899692317791814 1101 84-85 3.1571874806363844 1101 86-87 3.209823270410155 1101 88-89 3.4404034360346323 1101 90-91 3.2344554026460397 1101 92-93 3.2708624876470953 1101 94-95 3.1211493718338534 1101 96-97 3.222164904210617 1101 98-99 2.9322599540657475 1101 100-101 1.9693337769051267 1102 1 0.5238827775016226 1102 2 0.3356011256355309 1102 3 0.19577156978992605 1102 4 0.059184247059768325 1102 5 0.18321355767724867 1102 6 0.27804704376198686 1102 7 0.2413230016901622 1102 8 0.25129167997868507 1102 9 0.268221880389234 1102 10-11 0.4485235543453143 1102 12-13 0.4260491269543465 1102 14-15 0.43899432702011865 1102 16-17 0.5773523254159372 1102 18-19 0.5079312369282007 1102 20-21 0.37298225438708243 1102 22-23 0.36244078019091575 1102 24-25 0.4240934215486831 1102 26-27 0.4541371953220974 1102 28-29 0.35240331161574545 1102 30-31 0.2541266038700556 1102 32-33 0.23046868080672311 1102 34-35 0.3571576245815393 1102 36-37 0.62134381010803 1102 38-39 0.7672530659037946 1102 40-41 0.734746236212807 1102 42-43 1.0362301142433736 1102 44-45 1.0176819808348583 1102 46-47 0.9659427874798112 1102 48-49 1.1343363457356688 1102 50-51 1.285248525691646 1102 52-53 1.1422939821871765 1102 54-55 1.193335384280445 1102 56-57 1.5170730243104735 1102 58-59 1.4196795806240239 1102 60-61 1.5627105445296827 1102 62-63 1.6853386433508923 1102 64-65 2.1206657116334497 1102 66-67 2.18587565214294 1102 68-69 2.255500545220375 1102 70-71 2.0918862669254352 1102 72-73 2.3589547824022574 1102 74-75 2.3834586689579815 1102 76-77 2.6098273462543524 1102 78-79 3.0686483039307113 1102 80-81 3.410629130155044 1102 82-83 3.4774268329140483 1102 84-85 3.7416461333643305 1102 86-87 3.8279751504243507 1102 88-89 3.9913812379735276 1102 90-91 4.069683736731671 1102 92-93 3.8404185159424404 1102 94-95 3.9465371056969634 1102 96-97 3.496409211818163 1102 98-99 2.764828445486094 1102 100-101 2.0265293043776937 1103 1 0.321689128403321 1103 2 0.373869532947527 1103 3 0.23580577937046598 1103 4 0.10703761239525988 1103 5 0.20832163926746716 1103 6 0.23086705310500832 1103 7 0.17920325866248987 1103 8 0.1713743675817554 1103 9 0.32281229538992307 1103 10-11 0.3693960431500898 1103 12-13 0.3214504237889315 1103 14-15 0.3054034584927052 1103 16-17 0.433863860621436 1103 18-19 0.4976814710714308 1103 20-21 0.2887337308260882 1103 22-23 0.42318012577064223 1103 24-25 0.46579692737011413 1103 26-27 0.5147652751287666 1103 28-29 0.5613298817791303 1103 30-31 0.5002091300448583 1103 32-33 0.7809626987748004 1103 34-35 0.7878708381868478 1103 36-37 0.8106862624180522 1103 38-39 0.7917619844025481 1103 40-41 0.8485635873566437 1103 42-43 1.0484992816306473 1103 44-45 0.9855306106660251 1103 46-47 1.1744659934121202 1103 48-49 1.0261401868613476 1103 50-51 1.0066690655184978 1103 52-53 1.1838521612846051 1103 54-55 1.306324022166507 1103 56-57 1.5003817133416284 1103 58-59 1.718903486374689 1103 60-61 1.7916094241721154 1103 62-63 1.9972397253950582 1103 64-65 1.9816331417264479 1103 66-67 2.228745487496429 1103 68-69 2.5309857228234627 1103 70-71 2.491277855769713 1103 72-73 2.752687815145979 1103 74-75 2.6987948495115752 1103 76-77 2.7430852431826125 1103 78-79 3.054112638296129 1103 80-81 3.094189581221123 1103 82-83 3.4280460919665714 1103 84-85 3.325055518952258 1103 86-87 3.438995065346287 1103 88-89 3.649444280458681 1103 90-91 3.5652147335388182 1103 92-93 3.635018699210997 1103 94-95 3.6726770483135507 1103 96-97 3.2100504368811595 1103 98-99 2.8551583970142644 1103 100-101 2.0515224044547535 1104 1 0.3462351053678461 1104 2 0.29490238390183876 1104 3 0.4224747210275268 1104 4 0.3228249029195709 1104 5 0.36366507943166937 1104 6 0.3440680901657558 1104 7 0.40418990870790594 1104 8 0.3091720107740983 1104 9 0.5241230327000963 1104 10-11 0.4500211977534292 1104 12-13 0.4352904049245794 1104 14-15 0.6100600860998355 1104 16-17 0.5845182226730046 1104 18-19 0.5449432005438481 1104 20-21 0.5401364477785435 1104 22-23 0.7341225438635135 1104 24-25 0.6355257284495082 1104 26-27 0.6623649443249633 1104 28-29 0.812549815478846 1104 30-31 0.8421888221676994 1104 32-33 1.010868151795222 1104 34-35 0.9742383967706729 1104 36-37 0.8802424658763428 1104 38-39 1.0118050152079974 1104 40-41 0.894639418287916 1104 42-43 1.2036288434631146 1104 44-45 1.1897747500907698 1104 46-47 1.0388369329877989 1104 48-49 0.9928834019051536 1104 50-51 0.9321439282367159 1104 52-53 1.1721889154088458 1104 54-55 1.3171300604321914 1104 56-57 1.644202234140355 1104 58-59 1.8330973733612232 1104 60-61 1.9469399080534053 1104 62-63 1.773349475874987 1104 64-65 1.8921337306393369 1104 66-67 2.06355786357706 1104 68-69 2.4738985463742402 1104 70-71 2.450134681069784 1104 72-73 2.6925791338583807 1104 74-75 2.636922120473894 1104 76-77 2.66763462864618 1104 78-79 2.910104351058923 1104 80-81 2.949099643688342 1104 82-83 3.215446844347884 1104 84-85 3.475777652329814 1104 86-87 3.4090219007981055 1104 88-89 3.7731760846051294 1104 90-91 3.4166655681327303 1104 92-93 3.796787359397795 1104 94-95 3.742062029509997 1104 96-97 3.3187784667627316 1104 98-99 2.958167922274132 1104 100-101 1.9537488121100433 1105 1 0.5172490830440957 1105 2 0.48171105193526387 1105 3 0.3989173426170076 1105 4 0.43323498433856855 1105 5 0.4746400732035383 1105 6 0.4717001893770103 1105 7 0.5599971543659237 1105 8 0.5074653888433076 1105 9 0.4828284349077876 1105 10-11 0.49902610635800215 1105 12-13 0.4908057093639613 1105 14-15 0.7806691400868644 1105 16-17 0.7387912913241408 1105 18-19 0.8774763048648353 1105 20-21 0.7547174336591667 1105 22-23 0.690662099536901 1105 24-25 0.7549316486395412 1105 26-27 0.7168311802412006 1105 28-29 0.873866708834214 1105 30-31 0.8139412998968965 1105 32-33 1.0215847257827377 1105 34-35 0.9879844703822229 1105 36-37 0.9970703782604531 1105 38-39 0.9644637030114609 1105 40-41 1.189175214989561 1105 42-43 1.2817551171823922 1105 44-45 1.2671121273280406 1105 46-47 1.421283452967721 1105 48-49 1.4122825416232487 1105 50-51 1.4732246364227244 1105 52-53 1.4670721160658644 1105 54-55 1.7253872277338616 1105 56-57 1.9289881502621036 1105 58-59 1.8742830264920762 1105 60-61 2.3279893841667096 1105 62-63 2.2202607613991354 1105 64-65 2.439844921389245 1105 66-67 2.655779043333464 1105 68-69 2.983738611040131 1105 70-71 3.107482579466314 1105 72-73 3.040932736231696 1105 74-75 3.232858656018422 1105 76-77 3.3568366509028884 1105 78-79 3.614241219836984 1105 80-81 3.7448952558849626 1105 82-83 3.868843308446049 1105 84-85 3.9772677372228493 1105 86-87 4.010532327106947 1105 88-89 4.1073458348628265 1105 90-91 4.1522071640425064 1105 92-93 4.077333385469851 1105 94-95 4.0049146426200775 1105 96-97 3.784425186652294 1105 98-99 3.2081003080174657 1105 100-101 2.449192944449618 1106 1 0.29549943272556334 1106 2 0.24243244308055623 1106 3 0.16298861702277634 1106 4 0.10423401835070223 1106 5 0.1758275707578747 1106 6 -0.16652162568273354 1106 7 0.2963503698625516 1106 8 -0.13411359850370985 1106 9 0.31912763487954976 1106 10-11 0.23630733005516902 1106 12-13 0.3482028055691373 1106 14-15 0.4997364285458872 1106 16-17 0.028531832660981138 1106 18-19 0.4038417697628063 1106 20-21 0.5119130458184884 1106 22-23 0.4890475225574846 1106 24-25 0.47434833084334826 1106 26-27 0.5034215192883593 1106 28-29 0.560347539844642 1106 30-31 0.4293450084002757 1106 32-33 0.5940863734864266 1106 34-35 0.5540226694862014 1106 36-37 0.6114578429758097 1106 38-39 0.7455177384852334 1106 40-41 0.9301672246816608 1106 42-43 0.41830851237270394 1106 44-45 0.9486319124321838 1106 46-47 1.0287784714933537 1106 48-49 0.8077932875669092 1106 50-51 0.8970548818941317 1106 52-53 1.0603865247838513 1106 54-55 0.9301693663957025 1106 56-57 1.6008108443017512 1106 58-59 1.6810137138319625 1106 60-61 1.8194168173013985 1106 62-63 1.8247711152698791 1106 64-65 1.8305149862769312 1106 66-67 1.9227148093603077 1106 68-69 2.157548140330128 1106 70-71 2.0172143125897293 1106 72-73 2.0010126546559803 1106 74-75 2.3628940308163457 1106 76-77 2.541612904634679 1106 78-79 2.8885176112930147 1106 80-81 2.544154882579374 1106 82-83 2.640144626071912 1106 84-85 2.7164215694478635 1106 86-87 2.3313551009422575 1106 88-89 2.3735668926310503 1106 90-91 2.5880612346570633 1106 92-93 2.651963620813346 1106 94-95 2.6263786014516537 1106 96-97 2.419903546299013 1106 98-99 2.6246835345090123 1106 100-101 1.9889016442483642 1107 1 0.023322087659774837 1107 2 -0.031415505021719525 1107 3 -0.05645258411831833 1107 4 0.006152179020723736 1107 5 -0.1249284049878625 1107 6 -0.13954670589189533 1107 7 -0.06277278368270345 1107 8 -0.11611998878235141 1107 9 0.01044134941371766 1107 10-11 -0.054023013224380634 1107 12-13 -0.04129212281563355 1107 14-15 0.07269403082898407 1107 16-17 -0.06116099444115264 1107 18-19 0.06886979927319459 1107 20-21 0.0633870256234843 1107 22-23 0.1563834256326544 1107 24-25 0.09192904217142228 1107 26-27 0.10711341783679273 1107 28-29 0.17902724758265265 1107 30-31 0.18082556404348082 1107 32-33 0.31373836047609416 1107 34-35 0.21009735513562333 1107 36-37 0.34368545919099347 1107 38-39 0.43228203115462804 1107 40-41 0.4609479108401473 1107 42-43 0.5911104864846877 1107 44-45 0.3766168469600828 1107 46-47 0.43696257455562915 1107 48-49 0.4806965447042941 1107 50-51 0.7159268195234354 1107 52-53 0.6124814300020205 1107 54-55 0.6587951995005454 1107 56-57 1.1644228199613202 1107 58-59 1.0435021659608985 1107 60-61 1.2349709611093154 1107 62-63 1.358818178997499 1107 64-65 1.4878674253176811 1107 66-67 1.6310070358314235 1107 68-69 1.4940708181852962 1107 70-71 1.7879248489154698 1107 72-73 1.9882553395556073 1107 74-75 2.0367075124562426 1107 76-77 2.049830812796298 1107 78-79 2.272070192772471 1107 80-81 2.453581119909316 1107 82-83 2.5540379928084924 1107 84-85 2.3418475581052967 1107 86-87 2.302725356347434 1107 88-89 2.5167347951332886 1107 90-91 2.3470096182952442 1107 92-93 2.5526319943005493 1107 94-95 2.385508576484254 1107 96-97 2.01557944137358 1107 98-99 1.7519408374214098 1107 100-101 1.1318422268260582 1108 1 0.29193715303263446 1108 2 0.270172026016791 1108 3 0.12911714217180048 1108 4 0.19716599949228453 1108 5 0.07690674750062243 1108 6 0.30786264684110165 1108 7 0.233840815909943 1108 8 0.3012526146435093 1108 9 0.38878647054477256 1108 10-11 0.29681343194059195 1108 12-13 0.3224896985463772 1108 14-15 0.23877594539235503 1108 16-17 0.29402084217133506 1108 18-19 0.3926991717595314 1108 20-21 0.3915777808725238 1108 22-23 0.4037475690203678 1108 24-25 0.4372859773608795 1108 26-27 0.359263933516047 1108 28-29 0.47833244752467863 1108 30-31 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-0.7495693146677898 2315 92-93 -0.7714220544734101 2315 94-95 -0.6719167951271796 2315 96-97 -0.6124683023806394 2315 98-99 -0.8308210575585004 2315 100-101 -0.5175229673991497 2316 1 -0.9850534658391581 2316 2 -0.9098348961990261 2316 3 -0.6847318667949587 2316 4 -0.6995717256202667 2316 5 -0.5736032640149915 2316 6 -0.42489324627731406 2316 7 -0.6841775030676871 2316 8 -0.5460743821587783 2316 9 -0.676820395779373 2316 10-11 -0.9455972257020306 2316 12-13 -0.8024713539516455 2316 14-15 -0.9817883758048822 2316 16-17 -1.0373015419618596 2316 18-19 -0.954156439812607 2316 20-21 -1.105617998984954 2316 22-23 -1.1956755662776288 2316 24-25 -1.0566221515047616 2316 26-27 -1.0255494316605507 2316 28-29 -1.1581881834087895 2316 30-31 -0.9545096993162687 2316 32-33 -1.1049766978307503 2316 34-35 -0.9945631722190953 2316 36-37 -1.187386967858103 2316 38-39 -1.324230300403599 2316 40-41 -1.1184472747342653 2316 42-43 -0.8627749212451192 2316 44-45 -0.9881757361743126 2316 46-47 -0.7357489933007031 2316 48-49 -0.40534187437329905 2316 50-51 -0.4136382651969761 2316 52-53 -0.3274047866181853 2316 54-55 -0.11627397027718445 2316 56-57 -0.20048635274320858 2316 58-59 -0.1312778616828858 2316 60-61 -0.23118881564980143 2316 62-63 -0.015084873960702794 2316 64-65 0.02892564854087709 2316 66-67 0.1892019156999396 2316 68-69 0.27575584301887446 2316 70-71 0.36961524088960473 2316 72-73 0.35259101218495914 2316 74-75 0.5280958650777805 2316 76-77 0.5774907837373497 2316 78-79 0.3213208434628818 2316 80-81 0.29452942731018084 2316 82-83 0.40977578768964307 2316 84-85 0.4082300410450266 2316 86-87 0.5991204671437096 2316 88-89 0.2423081745985698 2316 90-91 0.06749728030449198 2316 92-93 0.13221583386043712 2316 94-95 0.25410087401440506 2316 96-97 0.5278260701258528 2316 98-99 0.4176085860928911 2316 100-101 0.8003043058673542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6288.0 3 2313.0 4 2579.0 5 3030.0 6 3744.0 7 4770.0 8 6269.0 9 8069.0 10 10548.0 11 13476.0 12 16508.0 13 20333.0 14 23609.0 15 26950.0 16 31022.0 17 35316.0 18 36311.0 19 37347.0 20 40996.0 21 45789.0 22 51116.0 23 56788.0 24 63234.0 25 70244.0 26 77344.0 27 84491.0 28 93640.0 29 102260.0 30 112303.0 31 123493.0 32 135969.0 33 150695.0 34 164853.0 35 172288.0 36 159383.0 37 102445.0 38 22270.0 39 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52469681591559 18.22146534788579 14.31771853561874 23.93611930057988 2 15.66749946995557 19.31623560321417 40.29050684524639 24.72575808158387 3 19.632560928963038 22.975826749715484 29.257046792876945 28.134565528444533 4 13.133757967799628 15.860980462627083 36.22506350862296 34.780198060950326 5 14.255854718800142 36.66856710720043 34.173164299618385 14.902413874381038 6 33.636226236863855 35.14176408076514 16.88100129885465 14.341008383516355 7 29.398778128336666 31.80299503534946 20.16574650861766 18.632480327696214 8 26.611911124879757 35.112557868047396 19.27969953715007 18.995831469922784 9 26.14136981850757 16.820574525325497 19.71563211149934 37.32242354466759 10-11 25.35276947829171 26.074199058273962 27.183402350112225 21.389629113322105 12-13 26.132175568608133 23.22488064033726 27.455366734364013 23.187577056690586 14-15 22.71092859483559 24.905420808933034 25.547761311019634 26.835889285211746 16-17 22.734794140873532 27.999225645305415 26.317034504820242 22.94894570900081 18-19 22.6940812081185 26.692158463088322 28.30399203326273 22.309768295530443 20-21 23.07572255290615 25.99412560532473 28.634274070733568 22.295877771035553 22-23 22.77021515214119 25.84151344331966 28.54148688132692 22.84678452321223 24-25 22.705509299521157 25.980855267398866 28.634241817538264 22.679393615541713 26-27 22.390839510090593 26.393241267538592 28.347608820484922 22.868310401885893 28-29 22.463940403535045 26.76245254601729 28.219710966022486 22.553896084425176 30-31 22.628298376763126 26.29896845633004 28.19555058988696 22.877182577019873 32-33 22.30304678703467 26.238227217447907 28.304943606568365 23.153782388949054 34-35 22.917608116180183 26.421098857511172 28.04787639682776 22.613416629480888 36-37 22.990092539206504 26.049075953736534 28.064992300598245 22.895839206458714 38-39 22.505064821691363 26.41336971286203 28.096528820735767 22.985036644710842 40-41 23.015656064204922 26.229820881330518 28.1057536189393 22.648769435525256 42-43 22.711923302362724 26.247241905241225 27.989406461076722 23.051428331319325 44-45 22.72477159725768 26.527860496045218 28.053496155931622 22.693871750765474 46-47 23.093655634770574 26.66824682255282 27.411282076948083 22.82681546572852 48-49 22.867431607196647 26.673169699894146 27.458868944365072 23.000529748544135 50-51 22.964954058619522 26.897944133846114 27.433604295101254 22.703497512433113 52-53 23.129159528066324 26.57150316380249 27.50112242638606 22.79821488174512 54-55 22.796715411773842 26.741913104810205 27.59320374346938 22.868167739946575 56-57 22.759623498864006 26.993266648962848 27.432840577144376 22.814269275028767 58-59 22.79347165683711 26.629666364690824 27.455150922615662 23.1217110558564 60-61 22.570683868701664 26.757802265037423 27.706981260967456 22.96453260529346 62-63 22.59486188773882 26.757161498905642 27.76267012631885 22.88530648703669 64-65 22.53471257541427 26.736950637482053 27.606266875930764 23.12206991117291 66-67 22.475813530921172 26.76556444457453 27.517683556222345 23.240938468281954 68-69 22.812528989285678 26.43598611016365 27.669534063546642 23.08195083700403 70-71 22.74394624010515 26.480884025165192 27.332738613591665 23.44243112113799 72-73 22.70464985624112 26.60419804261306 27.32910633436097 23.362045766784853 74-75 22.74180645097326 26.855512296431566 27.114020512111125 23.288660740484048 76-77 22.870387658453808 26.60588832209328 27.188635300899072 23.33508871855384 78-79 22.91308507466668 26.71670332155357 27.074817278319763 23.295394325459988 80-81 23.04585782928041 26.63172219737055 26.91050682857106 23.411913144777984 82-83 23.00849024044901 26.694589767426052 26.96440973164835 23.332510260476592 84-85 23.009710410843596 26.646210527334535 26.922413257919906 23.421665803901963 86-87 23.09782878086846 26.619232470520032 26.77367213283463 23.509266615776873 88-89 23.089986829652567 26.606887437792594 26.6088704261135 23.694255306441335 90-91 23.05596733643131 26.581055592897172 26.622276963698987 23.740700106972525 92-93 23.244046220969963 26.183668184594 26.75157491550918 23.820710678926854 94-95 23.01023198978808 26.058652453956515 26.83136941423328 24.099746142022124 96-97 23.248292462282137 25.79135036417084 26.713864554699278 24.246492618847743 98-99 23.130720885919374 25.90552169947255 26.36897601453367 24.594781400074407 100-101 24.264210841979818 24.878907460179537 26.514096880175984 24.342784817664658 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 180.0 1 146.5 2 163.5 3 238.0 4 214.0 5 197.0 6 334.5 7 684.5 8 1140.5 9 1373.5 10 1219.5 11 1039.0 12 1054.5 13 1163.0 14 1329.0 15 1477.5 16 1650.5 17 1842.0 18 2077.5 19 2413.0 20 2852.0 21 3365.5 22 4065.5 23 5164.5 24 6597.0 25 8411.5 26 10819.5 27 13592.5 28 16263.0 29 19044.5 30 22240.0 31 26833.0 32 32326.5 33 35314.0 34 36778.0 35 39378.5 36 43437.5 37 48128.0 38 52011.0 39 55155.0 40 58178.5 41 61806.5 42 64764.5 43 67261.5 44 70394.5 45 73896.0 46 76739.0 47 78644.0 48 79616.5 49 79375.0 50 78458.5 51 77241.5 52 76350.5 53 76303.5 54 76300.0 55 73284.5 56 67595.0 57 61713.5 58 56982.5 59 51074.0 60 42810.0 61 35789.0 62 30202.0 63 24086.5 64 18352.0 65 14173.5 66 10781.0 67 8275.0 68 6426.0 69 4906.0 70 3795.5 71 2811.0 72 2166.0 73 1700.5 74 1298.0 75 982.5 76 708.5 77 497.0 78 345.5 79 232.0 80 141.0 81 89.0 82 61.0 83 43.0 84 23.5 85 14.0 86 11.0 87 6.0 88 4.5 89 4.5 90 3.0 91 2.0 92 2.5 93 2.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37378112797117763 2 0.028559937158696952 3 0.03408309855963504 4 0.04357160660740047 5 0.06132125350272287 6 0.05202157148575874 7 0.03686828251395424 8 0.038662129806566616 9 0.035074435221341876 10-11 0.03545208728294448 12-13 0.027993459066293042 14-15 0.025963579235179043 16-17 0.021691390288299583 18-19 0.02579835645822791 20-21 0.03243087079012364 22-23 0.03136872436686632 24-25 0.04014913479912687 26-27 0.03474398966743959 28-29 0.030330181197459158 30-31 0.033068158644078034 32-33 0.028158681843244184 34-35 0.028654350174097603 36-37 0.030920262543713228 38-39 0.01468122389480124 40-41 0.012226485494384315 42-43 0.012674947317537407 44-45 0.01031462193252113 46-47 0.019708716964885912 48-49 0.017277581818319145 50-51 0.010291018678670966 52-53 0.008166725832156317 54-55 0.007387818455100947 56-57 0.007765470516703551 58-59 0.003823727123726368 60-61 0.007222595678149807 62-63 0.010385431694071618 64-65 0.006396481793394111 66-67 0.0033280587928729505 68-69 0.005310732116286623 70-71 0.012627740809837083 72-73 0.01047984470947227 74-75 0.011211545578827316 76-77 0.005121906085485321 78-79 0.008095916070605828 80-81 0.01083389351722471 82-83 0.01288737660218887 84-85 0.007623850993602573 86-87 0.002313118877315951 88-89 0.015884989841159543 90-91 0.024476574242618787 92-93 0.010503447963322431 94-95 0.02055843410349177 96-97 0.018410538003126958 98-99 0.011140735817276827 100-101 0.11855914408936759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2118352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.183707037620806 #Duplication Level Percentage of deduplicated Percentage of total 1 79.98784482507797 49.73940709173322 2 11.831751642617284 14.714843557728038 3 3.633396582323657 6.778142058801209 4 1.5185261166166868 3.777103326586722 5 0.8526082950090151 2.6509172217342987 6 0.4765581506148706 1.778049145453529 7 0.3286149542268074 1.4304147229274675 8 0.23406124580631263 1.1643836750464236 9 0.16304638839811927 0.9124945964721706 >10 0.8989717225097026 9.933034155325462 >50 0.04842115696613027 2.036198439188211 >100 0.022764170064018107 2.613638227815209 >500 0.0022135054070402545 1.0000071865380762 >1k 0.0011449165898483424 1.22958783874196 >5k 7.632777265655616E-5 0.24177875590795744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.24046995022545828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4058 0.19156400824792102 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2691 0.12703271222157603 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.7206507700325535E-5 0.0 10-11 0.0 0.0 0.0 4.7206507700325535E-5 0.0 12-13 2.3603253850162767E-5 0.0 0.0 4.7206507700325535E-5 0.0 14-15 4.7206507700325535E-5 0.0 0.0 4.7206507700325535E-5 0.0 16-17 4.7206507700325535E-5 0.0 0.0 4.7206507700325535E-5 0.0 18-19 7.08097615504883E-5 0.0 0.0 4.7206507700325535E-5 0.0 20-21 9.441301540065107E-5 0.0 0.0 7.08097615504883E-5 0.0 22-23 9.441301540065107E-5 0.0 0.0 2.3603253850162768E-4 0.0 24-25 1.416195231009766E-4 0.0 0.0 4.484618231530926E-4 0.0 26-27 1.416195231009766E-4 0.0 0.0 8.969236463061852E-4 0.0 28-29 1.8882603080130214E-4 0.0 0.0 0.002525548161967416 0.0 30-31 1.8882603080130214E-4 0.0 0.0 0.008756807178410387 0.0 32-33 1.8882603080130214E-4 0.0 0.0 0.020487624341941284 0.0 34-35 2.1242928465146492E-4 0.0 0.0 0.03911059162971971 0.0 36-37 2.3603253850162768E-4 0.0 0.0 0.07007806068113326 0.0 38-39 3.3044555390227876E-4 0.0 0.0 0.11862995385091807 0.0 40-41 3.3044555390227876E-4 0.0 0.0 0.17405039389110027 4.7206507700325535E-5 42-43 3.3044555390227876E-4 0.0 0.0 0.23275168621645506 4.7206507700325535E-5 44-45 3.3044555390227876E-4 0.0 0.0 0.3112561085220964 4.7206507700325535E-5 46-47 3.3044555390227876E-4 0.0 0.0 0.4019870163221221 4.7206507700325535E-5 48-49 3.3044555390227876E-4 0.0 0.0 0.5022772419314637 4.7206507700325535E-5 50-51 3.3044555390227876E-4 0.0 0.0 0.6095068241727531 4.7206507700325535E-5 52-53 3.3044555390227876E-4 0.0 0.0 0.7139276192058732 7.08097615504883E-5 54-55 3.3044555390227876E-4 0.0 0.0 0.8310705680642311 9.441301540065107E-5 56-57 3.3044555390227876E-4 0.0 0.0 0.9696452714185366 9.441301540065107E-5 58-59 3.3044555390227876E-4 0.0 0.0 1.1184165804361126 9.441301540065107E-5 60-61 3.3044555390227876E-4 0.0 0.0 1.279721217248125 9.441301540065107E-5 62-63 3.3044555390227876E-4 0.0 0.0 1.4435514022221048 9.441301540065107E-5 64-65 3.3044555390227876E-4 0.0 0.0 1.6223224468832376 9.441301540065107E-5 66-67 3.3044555390227876E-4 0.0 0.0 1.8264433861794451 9.441301540065107E-5 68-69 3.3044555390227876E-4 0.0 0.0 2.0525625580640043 9.441301540065107E-5 70-71 3.540488077524415E-4 0.0 0.0 2.2885478900579317 9.441301540065107E-5 72-73 4.2485856930292985E-4 0.0 0.0 2.531260149399156 9.441301540065107E-5 74-75 4.2485856930292985E-4 0.0 0.0 2.7799204287106205 9.441301540065107E-5 76-77 4.2485856930292985E-4 0.0 0.0 3.047109262294463 9.441301540065107E-5 78-79 4.2485856930292985E-4 0.0 0.0 3.347460667537784 9.441301540065107E-5 80-81 4.2485856930292985E-4 0.0 0.0 3.6651132578532746 1.1801626925081384E-4 82-83 4.2485856930292985E-4 0.0 0.0 3.9880057705235012 1.416195231009766E-4 84-85 4.2485856930292985E-4 0.0 0.0 4.312715733740191 1.416195231009766E-4 86-87 4.2485856930292985E-4 2.3603253850162767E-5 0.0 4.6499590247513165 1.416195231009766E-4 88-89 4.2485856930292985E-4 4.7206507700325535E-5 0.0 5.011017998897255 1.416195231009766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2765 0.0 54.681526 1 GTATCAA 5020 0.0 45.22512 1 ATCAACG 6505 0.0 34.55555 3 TATCAAC 6590 0.0 34.10984 2 TCAACGC 6610 0.0 33.86284 4 CAACGCA 6860 0.0 32.49022 5 AACGCAG 7165 0.0 31.168348 6 ACGCAGA 8355 0.0 26.66588 7 CGCAGAG 8305 0.0 26.597626 8 TGGTATC 1230 0.0 25.500153 2 GTGGTAT 1395 0.0 24.61698 1 GCAGAGT 9225 0.0 23.378075 9 GTACATG 5845 0.0 22.358494 1 TACATGG 5555 0.0 22.24299 2 GGACCGA 430 0.0 20.995066 6 CAGAGTA 8535 0.0 20.81528 10-11 GAGTACT 5960 0.0 20.802896 12-13 AGAGTAC 8325 0.0 20.570044 10-11 ACATGGG 5895 0.0 19.992716 3 GTGTAGC 1235 0.0 19.30988 1 >>END_MODULE