FastQCFastQC Report
Thu 26 May 2016
SRR938628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118352
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.24665400273420093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.2111547089435561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.14025053437766716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.053.3761941
GTATCAA49100.042.384811
ATCAACG65550.031.5195753
TATCAAC66800.031.0749032
TCAACGC66700.030.8337174
CAACGCA69400.029.7025765
AACGCAG71850.028.7558566
GTGTAAG9100.026.628481
ACGCAGA84400.024.423687
CGCAGAG84200.024.3124668
TGGTATC15400.023.7506122
GTGGTAT16950.022.4252821
GTACATG60250.021.844411
TACATGG58100.021.8293082
TAAGGTG11600.021.70115
ACATGGG59050.020.5913053
GCAGAGT96450.020.535149
GTAAGGT11500.020.2377434
GGACCGA5400.020.2300936
AGAGTAC87650.020.15834210-11
Produced by FastQC (version 0.11.5)