##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2118352 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63260355219529 31.0 31.0 34.0 30.0 34.0 2 31.904025393324623 31.0 31.0 34.0 30.0 34.0 3 31.958916648413485 31.0 31.0 34.0 30.0 34.0 4 35.64526905821129 37.0 35.0 37.0 33.0 37.0 5 35.52535461528584 37.0 35.0 37.0 33.0 37.0 6 35.56331242399752 37.0 35.0 37.0 33.0 37.0 7 35.48000049094768 37.0 35.0 37.0 33.0 37.0 8 35.44802610708702 37.0 35.0 37.0 33.0 37.0 9 36.96785378445131 39.0 37.0 39.0 33.0 39.0 10-11 36.88212723853259 39.0 37.0 39.0 33.0 39.0 12-13 36.74591852534423 39.0 36.5 39.0 32.0 39.0 14-15 37.69031492405417 39.5 36.5 41.0 32.0 41.0 16-17 37.77744303118651 39.0 37.0 41.0 32.0 41.0 18-19 37.90927381285074 40.0 37.0 41.0 33.0 41.0 20-21 37.84550844241184 40.0 37.0 41.0 32.0 41.0 22-23 37.702577758559485 39.5 37.0 41.0 32.0 41.0 24-25 37.551487901916204 39.0 36.5 41.0 32.0 41.0 26-27 37.29581226349539 39.0 36.0 41.0 31.0 41.0 28-29 37.12887116966397 39.0 36.0 40.5 31.0 41.0 30-31 36.842021533720555 39.0 36.0 40.0 30.0 41.0 32-33 36.71494562754443 39.0 35.0 40.0 30.0 41.0 34-35 36.54926589159875 39.0 35.0 40.0 30.0 41.0 36-37 36.39336899627635 38.0 35.0 40.0 30.0 41.0 38-39 36.1351477469278 38.0 35.0 40.0 30.0 41.0 40-41 35.86593823878185 38.0 35.0 40.0 29.0 41.0 42-43 35.5651900628413 38.0 34.0 40.0 27.5 41.0 44-45 35.2334918842572 38.0 34.0 40.0 27.0 41.0 46-47 34.77365895752925 38.0 33.0 40.0 25.5 41.0 48-49 34.30289914046391 38.0 33.0 40.0 24.0 41.0 50-51 33.83325504920806 37.0 32.5 40.0 23.5 41.0 52-53 33.13278128469678 37.0 31.5 39.0 21.5 40.0 54-55 32.60323898011285 36.0 31.0 39.0 19.0 40.0 56-57 32.1288253793515 36.0 30.5 39.0 17.5 40.0 58-59 31.4781792638806 35.0 29.5 38.5 13.0 40.0 60-61 30.862403651517784 35.0 28.5 38.0 8.0 40.0 62-63 30.21772750704321 34.0 28.0 38.0 7.5 40.0 64-65 29.438448614772238 34.0 26.5 37.5 2.0 39.5 66-67 28.659439743725308 33.5 26.0 37.0 2.0 39.0 68-69 27.80778359781566 32.5 25.0 36.0 2.0 39.0 70-71 26.90767988511824 31.5 22.5 35.5 2.0 38.5 72-73 26.223854911742713 31.0 21.5 35.0 2.0 38.0 74-75 25.423085020808628 31.0 19.5 35.0 2.0 37.0 76-77 24.37908501514385 29.5 18.5 33.5 2.0 36.0 78-79 24.784639191220347 31.0 18.5 34.0 2.0 36.0 80-81 24.315983368203206 30.5 15.0 34.0 2.0 36.0 82-83 23.528226659214333 30.0 7.5 34.0 2.0 35.0 84-85 22.61715333429005 29.0 2.0 34.0 2.0 35.0 86-87 21.566112950066845 29.0 2.0 33.5 2.0 35.0 88-89 20.44169878282741 26.5 2.0 33.0 2.0 35.0 90-91 19.43349122336609 25.5 2.0 32.5 2.0 34.0 92-93 18.211222450282108 24.5 2.0 32.0 2.0 34.0 94-95 16.93209981155162 19.0 2.0 31.5 2.0 34.0 96-97 15.4780376443575 4.5 2.0 31.0 2.0 34.0 98-99 13.72932779821295 2.0 2.0 30.5 2.0 34.0 100-101 10.230229206477489 2.0 2.0 23.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25023296839072984 1101 2 0.25784957767843153 1101 3 0.25513293160292605 1101 4 0.19600284929930467 1101 5 0.03778918646140994 1101 6 0.21466398425661737 1101 7 0.16264672655890422 1101 8 0.28039863044960356 1101 9 0.2581121961297441 1101 10-11 0.24315529938935754 1101 12-13 0.1922035037151275 1101 14-15 0.3391234634306528 1101 16-17 0.3316787787983557 1101 18-19 0.3250124363511375 1101 20-21 0.39130275216551524 1101 22-23 0.315309043714457 1101 24-25 0.19427456536674015 1101 26-27 0.4413392174362727 1101 28-29 0.31802262434852224 1101 30-31 0.4901533104856952 1101 32-33 0.4957402909562987 1101 34-35 0.20992563431258304 1101 36-37 0.3151180394517681 1101 38-39 0.5193095221972186 1101 40-41 0.6502685016592409 1101 42-43 0.5841740911748303 1101 44-45 0.7591422645970454 1101 46-47 0.6563388176282245 1101 48-49 0.5592643701991165 1101 50-51 0.8431057189458997 1101 52-53 0.949592733843069 1101 54-55 0.932452638877372 1101 56-57 1.0771460611402333 1101 58-59 1.1462688137094155 1101 60-61 1.1133981033816767 1101 62-63 1.2734008014811167 1101 64-65 1.208128775429575 1101 66-67 1.4094433023753687 1101 68-69 1.4528539463128674 1101 70-71 1.5779400068143161 1101 72-73 1.6850744192695366 1101 74-75 1.7106304190985995 1101 76-77 1.6352189788821896 1101 78-79 1.7547285835284043 1101 80-81 1.851686075088537 1101 82-83 2.056694144226057 1101 84-85 2.3083251969089353 1101 86-87 2.414964742581194 1101 88-89 2.2491923500383777 1101 90-91 2.741170856298613 1101 92-93 2.9274886678161884 1101 94-95 2.829379089897209 1101 96-97 3.115948634212563 1101 98-99 3.033874380863681 1101 100-101 2.5225975794895685 1102 1 0.26860489812996136 1102 2 0.1986730575878859 1102 3 0.16465928589095213 1102 4 0.18716417136710817 1102 5 0.1252625162292773 1102 6 0.19742018673003514 1102 7 0.17757408981348988 1102 8 0.30818200474525526 1102 9 0.2553120969228786 1102 10-11 0.33742436259097275 1102 12-13 0.2750312810514757 1102 14-15 0.4224298740770678 1102 16-17 0.30203076820846064 1102 18-19 0.39094108246040804 1102 20-21 0.3299886301347783 1102 22-23 0.4087223113962679 1102 24-25 0.4016003595091391 1102 26-27 0.36201387026988385 1102 28-29 0.3697975195177605 1102 30-31 0.37798948329768933 1102 32-33 0.4065020139607398 1102 34-35 0.4165431224374174 1102 36-37 0.3017994334047245 1102 38-39 0.39794453043964495 1102 40-41 0.5416468335120825 1102 42-43 0.6122500334880954 1102 44-45 0.5604476986889466 1102 46-47 0.5004617016465502 1102 48-49 0.7868246338646472 1102 50-51 0.8822681127497916 1102 52-53 0.8853127834632772 1102 54-55 0.8784902448218972 1102 56-57 0.9812262056988175 1102 58-59 1.2535439980922582 1102 60-61 1.3358019998719328 1102 62-63 1.4385197543329014 1102 64-65 1.581860360468962 1102 66-67 1.6623320315567582 1102 68-69 1.9591129731001864 1102 70-71 2.167531231325203 1102 72-73 2.1316173890309393 1102 74-75 2.1048826473221993 1102 76-77 2.1982103091829934 1102 78-79 2.40876726959506 1102 80-81 2.620742882342295 1102 82-83 2.8848623749243743 1102 84-85 2.9099639132492 1102 86-87 2.7478094492385168 1102 88-89 2.4382840599962385 1102 90-91 2.6648340846184375 1102 92-93 2.9342907956064614 1102 94-95 2.62411039169805 1102 96-97 2.7309597320900334 1102 98-99 2.329599952692117 1102 100-101 1.7903967950173314 1103 1 0.2258926720858021 1103 2 0.20804064481540152 1103 3 0.16070945416236881 1103 4 0.11268484460966732 1103 5 0.07494644092609093 1103 6 0.0812077314453532 1103 7 0.1461525529297134 1103 8 0.195708409723359 1103 9 0.27833812547586234 1103 10-11 0.3256708573578706 1103 12-13 0.42051530471082543 1103 14-15 0.47107840779442256 1103 16-17 0.28036459731786323 1103 18-19 0.38989293410659087 1103 20-21 0.3320906036675595 1103 22-23 0.5048425213686158 1103 24-25 0.37554115166776825 1103 26-27 0.5164468411052496 1103 28-29 0.5068341176998956 1103 30-31 0.49273329230699403 1103 32-33 0.49245750723864745 1103 34-35 0.7017789181435532 1103 36-37 0.7165527175512878 1103 38-39 0.509381330758039 1103 40-41 0.6128859316854474 1103 42-43 0.5193110131181982 1103 44-45 0.7487020584390578 1103 46-47 0.7760265612318236 1103 48-49 0.8745558380513714 1103 50-51 0.7796940750800303 1103 52-53 0.8480518577939762 1103 54-55 0.8731573320553068 1103 56-57 1.096353871303677 1103 58-59 1.2389964627323486 1103 60-61 1.445690575147765 1103 62-63 1.568572206133048 1103 64-65 1.3984291345864364 1103 66-67 1.4167861201817011 1103 68-69 1.5210344401074103 1103 70-71 1.8763094815295212 1103 72-73 2.1680119279664254 1103 74-75 1.8050098288486964 1103 76-77 1.9368544397483198 1103 78-79 2.0461196024708777 1103 80-81 2.3465718381553344 1103 82-83 2.5832304631575163 1103 84-85 2.786976941253286 1103 86-87 2.59794260691368 1103 88-89 2.7706717718785754 1103 90-91 3.22126843564482 1103 92-93 3.456917278883463 1103 94-95 3.1260203853255533 1103 96-97 2.796419306179514 1103 98-99 2.7541035452967204 1103 100-101 2.4753415657014646 1104 1 0.22319377869188983 1104 2 0.1963016545164642 1104 3 0.23042541855531695 1104 4 0.07272926273403613 1104 5 0.12266622483123513 1104 6 0.14401082125970532 1104 7 0.21573397130782013 1104 8 0.19649774634373074 1104 9 0.2149984462215997 1104 10-11 0.24819447815657014 1104 12-13 0.26813117922703356 1104 14-15 0.4049585095550583 1104 16-17 0.40494055312776567 1104 18-19 0.34741944814292225 1104 20-21 0.36108387457286995 1104 22-23 0.4806546361309856 1104 24-25 0.3261485123114838 1104 26-27 0.44956152553704243 1104 28-29 0.4008855094937047 1104 30-31 0.4486296069620863 1104 32-33 0.5435061588782943 1104 34-35 0.4181364844433446 1104 36-37 0.6340157139089797 1104 38-39 0.6195397342729052 1104 40-41 0.7009959309260125 1104 42-43 0.7215064652237118 1104 44-45 0.5608215050024654 1104 46-47 0.62736128759812 1104 48-49 0.9034367879502199 1104 50-51 0.8921983631113477 1104 52-53 0.8466648378765811 1104 54-55 1.0082407336416637 1104 56-57 1.1980444469632872 1104 58-59 1.451104866556168 1104 60-61 1.41132961589647 1104 62-63 1.1628226861633273 1104 64-65 1.3872052063650422 1104 66-67 1.4774389653515279 1104 68-69 1.6598097843408972 1104 70-71 2.045436114444737 1104 72-73 1.992505621346222 1104 74-75 1.8801317524540693 1104 76-77 1.9478853193543522 1104 78-79 2.131031193779485 1104 80-81 2.2556539134169853 1104 82-83 2.271590598389217 1104 84-85 2.551592475517573 1104 86-87 2.120767192198013 1104 88-89 2.3060566761346344 1104 90-91 2.6045186726949687 1104 92-93 2.7682609430916933 1104 94-95 2.553582006902033 1104 96-97 2.6829013385060403 1104 98-99 2.660879601527787 1104 100-101 2.5467571950847976 1105 1 0.24015581490570526 1105 2 0.20169136785487396 1105 3 0.22661768836019647 1105 4 0.3011989787846332 1105 5 0.16488932826086256 1105 6 0.09155110983354575 1105 7 0.2534762493787852 1105 8 0.2262552568591758 1105 9 0.342234383027602 1105 10-11 0.3071938214411958 1105 12-13 0.32890344450652265 1105 14-15 0.4123319870915054 1105 16-17 0.3535600033305357 1105 18-19 0.44487597122357414 1105 20-21 0.35249981614806103 1105 22-23 0.4641641855495777 1105 24-25 0.3790135283522531 1105 26-27 0.40987909922515087 1105 28-29 0.4926525309542882 1105 30-31 0.5991927480119017 1105 32-33 0.655609263191657 1105 34-35 0.5241155464983791 1105 36-37 0.664355659345226 1105 38-39 0.6376164534082349 1105 40-41 0.8312323217950706 1105 42-43 0.7179080535302091 1105 44-45 0.8133905610617234 1105 46-47 0.859104105059437 1105 48-49 1.1163338931458142 1105 50-51 1.1518504410974444 1105 52-53 1.235274635429974 1105 54-55 1.1566130775441579 1105 56-57 1.4238130210757447 1105 58-59 1.6854560706232284 1105 60-61 1.7427319431982298 1105 62-63 1.793457715452682 1105 64-65 1.9620141913283895 1105 66-67 2.1272783481609743 1105 68-69 2.5119445473634023 1105 70-71 2.572015183508796 1105 72-73 2.373831363598388 1105 74-75 2.3388248698179446 1105 76-77 2.4874063285323693 1105 78-79 2.472760694089178 1105 80-81 2.691355897962083 1105 82-83 2.9685834019467556 1105 84-85 3.156219823827371 1105 86-87 3.247598491008837 1105 88-89 3.4221534974646275 1105 90-91 3.2235817600343033 1105 92-93 3.095015028326035 1105 94-95 2.823435838211509 1105 96-97 2.863596439729344 1105 98-99 3.076103608512435 1105 100-101 2.433008207394021 1106 1 0.03555340829923992 1106 2 0.05924038272372201 1106 3 0.10731550142197577 1106 4 0.14086973728075947 1106 5 0.15296006864951295 1106 6 0.11347515970889788 1106 7 0.11798532575167542 1106 8 0.014422711124367993 1106 9 -0.07833018662442015 1106 10-11 0.1422027677625053 1106 12-13 0.20190231892733124 1106 14-15 0.18140497250040966 1106 16-17 0.15621171291422087 1106 18-19 0.2991520212462504 1106 20-21 0.19451927171147076 1106 22-23 0.31813793277687097 1106 24-25 0.2071223558240547 1106 26-27 -0.002877100475771499 1106 28-29 0.24272887391910558 1106 30-31 0.39362214764929604 1106 32-33 0.2404019364153811 1106 34-35 -0.008396486150331839 1106 36-37 0.16211409179013714 1106 38-39 0.22148217342213172 1106 40-41 0.4497512260778791 1106 42-43 0.557741088780709 1106 44-45 0.58678095212953 1106 46-47 0.1726657614025342 1106 48-49 0.2868662376267608 1106 50-51 0.5196186715289031 1106 52-53 0.5728697476387836 1106 54-55 0.4527589470828559 1106 56-57 0.7425953469504876 1106 58-59 0.9137689141186094 1106 60-61 1.1531863449647837 1106 62-63 1.1022798739406774 1106 64-65 1.1688612345538552 1106 66-67 1.1644538234247044 1106 68-69 1.1465666381785375 1106 70-71 1.1323733125767426 1106 72-73 1.5170488474416892 1106 74-75 1.4647532616514702 1106 76-77 1.596522292345039 1106 78-79 1.908870264274448 1106 80-81 2.011459743575891 1106 82-83 2.234878884514856 1106 84-85 2.3653254103676815 1106 86-87 2.298571518540644 1106 88-89 2.5030069312484216 1106 90-91 2.761015995749151 1106 92-93 2.581290078601139 1106 94-95 2.335332489018789 1106 96-97 2.4075376663881567 1106 98-99 2.4994770430021145 1106 100-101 2.1639258229768856 1107 1 -0.011818136574905935 1107 2 0.03903063993914557 1107 3 0.029609690440668857 1107 4 0.060067802472637766 1107 5 -0.07065792352410938 1107 6 -0.0665962334863579 1107 7 -0.09842210864914591 1107 8 -0.04224438574320999 1107 9 0.09578723857211457 1107 10-11 -0.009160462077595355 1107 12-13 0.10519035141197719 1107 14-15 0.10584103296108793 1107 16-17 0.07711845675754603 1107 18-19 0.03597214240418367 1107 20-21 0.14305700748030858 1107 22-23 0.07612752896938701 1107 24-25 0.15207210262594373 1107 26-27 0.11813088860078125 1107 28-29 0.23459694295255673 1107 30-31 0.2051436019828401 1107 32-33 0.11135385135427356 1107 34-35 0.18777068828568844 1107 36-37 0.2449537806018256 1107 38-39 0.3408369438620724 1107 40-41 0.43478883212507924 1107 42-43 0.4227881655466348 1107 44-45 0.4010353475010362 1107 46-47 0.5628049518677258 1107 48-49 0.5750559377099549 1107 50-51 0.5451880059010179 1107 52-53 0.4923015574247316 1107 54-55 0.5262309008494199 1107 56-57 0.48196984112586705 1107 58-59 0.6073051863855277 1107 60-61 0.6822984121758964 1107 62-63 0.9117507249316148 1107 64-65 1.0012351820654395 1107 66-67 1.0224858224360602 1107 68-69 1.1900114817293321 1107 70-71 1.1832914639876861 1107 72-73 1.391199491958865 1107 74-75 1.4716732920892852 1107 76-77 1.519640067308135 1107 78-79 1.5909173378280315 1107 80-81 1.645732880086321 1107 82-83 1.9064161435354237 1107 84-85 2.2303494339076693 1107 86-87 2.3724166700220657 1107 88-89 2.2851575364617425 1107 90-91 2.1655262271298454 1107 92-93 2.162752620604774 1107 94-95 2.040807970306844 1107 96-97 2.1463441441934723 1107 98-99 2.0764113267582243 1107 100-101 1.7423996654073317 1108 1 0.09232417420447092 1108 2 0.10665818287447237 1108 3 0.10907550462266968 1108 4 0.036662299219621275 1108 5 0.05749376593575306 1108 6 0.10992024009797774 1108 7 0.14154645802668853 1108 8 0.07662276419245018 1108 9 0.1607184831445565 1108 10-11 0.15371695554726017 1108 12-13 0.07184457436596148 1108 14-15 0.25395367831491455 1108 16-17 0.11768508055182991 1108 18-19 0.08024251741768751 1108 20-21 0.16921663080080407 1108 22-23 0.22405107927986734 1108 24-25 0.3251721265858123 1108 26-27 0.44664689093822574 1108 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2316 90-91 0.7460326319175756 2316 92-93 0.9868903201237025 2316 94-95 1.3361839274469105 2316 96-97 1.226944122162065 2316 98-99 1.0092701070383452 2316 100-101 0.7403486940553972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 31.0 5 100.0 6 200.0 7 583.0 8 1281.0 9 2339.0 10 3785.0 11 5940.0 12 8501.0 13 11973.0 14 16289.0 15 21020.0 16 26196.0 17 32102.0 18 33384.0 19 33912.0 20 35792.0 21 39120.0 22 42437.0 23 47212.0 24 52042.0 25 57983.0 26 65250.0 27 74369.0 28 86344.0 29 98261.0 30 112401.0 31 126484.0 32 143227.0 33 163027.0 34 186873.0 35 206120.0 36 206471.0 37 143788.0 38 33151.0 39 352.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.784955283810056 19.493907445678836 15.230229003740947 23.490908266770163 2 16.357985640312876 20.440884318028168 38.95565568476672 24.245474356892235 3 19.86341075624952 23.566813732756398 29.92373208605679 26.64604342493729 4 13.912701949439942 16.94156589650823 35.71889846446672 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27.563478590904626 22.832584008701104 50-51 22.938114156665183 26.920525956026193 27.523825124436353 22.61753476287227 52-53 23.200794768763643 26.69358067025688 27.453369411693618 22.65225514928586 54-55 22.82765092864642 26.860856930292982 27.533950920338075 22.777541220722526 56-57 22.840160653187006 27.005261637348276 27.422921214226907 22.731656495237807 58-59 22.80159293639584 26.74076357470335 27.436139036383 23.021504452517807 60-61 22.588054298813418 26.89654505011443 27.609221696866243 22.90617895420591 62-63 22.659147299410108 26.838764284689233 27.684350853871308 22.81773756202935 64-65 22.536754457540862 26.840980131456178 27.52016488316252 23.102100527840438 66-67 22.457902658904906 26.835539901748284 27.492235055417357 23.21432238392945 68-69 22.816294564670407 26.568313847821344 27.534265264175577 23.08112632333267 70-71 22.647136367251957 26.656649433910694 27.284189884624798 23.41202431421255 72-73 22.660349787133402 26.749484444345832 27.227026941544707 23.36313882697605 74-75 22.672648360612403 27.020414926320086 26.924042840849864 23.382893872217647 76-77 22.624167277204165 26.83177772154958 27.335494761965904 23.208560239280345 78-79 22.661743657333624 26.900179951207353 27.29673821914394 23.14133817231508 80-81 22.7416943494149 26.941289599862532 27.139297763775243 23.17771828694732 82-83 22.727125741535 26.902475563835683 27.19707146045308 23.173327234176238 84-85 22.599672222548755 27.01973439309831 27.060783290384244 23.319810093968695 86-87 22.729135322106806 26.911087748087226 26.947650828015675 23.412126101790292 88-89 22.62138209324985 27.10264866273405 26.6019292355567 23.674040008459407 90-91 22.532637815254976 26.994181723767603 26.952828295941366 23.520352165036055 92-93 22.579501538823216 26.70358079996148 26.977946641697425 23.738971019517884 94-95 22.37084411412462 26.607205321497712 27.03534494519558 23.986605619182093 96-97 22.55487344758412 26.36453393121963 26.960728660942245 24.119863960253998 98-99 22.280975966222798 26.564801317250392 26.751479451951326 24.40274326457548 100-101 22.583816374151404 26.255587482905334 26.94135674399568 24.21923939894758 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 265.0 1 202.5 2 174.0 3 233.5 4 229.0 5 199.5 6 339.0 7 760.0 8 1213.5 9 1397.0 10 1281.5 11 1113.0 12 1144.0 13 1269.5 14 1405.0 15 1619.0 16 1822.5 17 1996.5 18 2231.0 19 2549.5 20 2970.5 21 3581.5 22 4387.0 23 5453.5 24 6953.5 25 8745.0 26 11286.0 27 14373.0 28 16947.5 29 19818.5 30 23158.5 31 27530.0 32 33437.5 33 36376.0 34 37326.0 35 40312.0 36 44864.0 37 49252.5 38 52884.0 39 55863.0 40 59190.5 41 63357.5 42 65972.0 43 68185.5 44 71259.0 45 74912.5 46 77209.0 47 79141.0 48 80407.5 49 79902.5 50 78862.0 51 77097.0 52 75946.0 53 76096.5 54 76283.5 55 72401.5 56 66398.5 57 60726.0 58 55305.5 59 48780.0 60 40252.0 61 33681.0 62 28467.0 63 22364.5 64 16682.5 65 12640.5 66 9638.0 67 7383.5 68 5641.5 69 4334.5 70 3394.5 71 2487.0 72 1887.5 73 1527.5 74 1168.0 75 841.5 76 609.5 77 409.0 78 256.0 79 164.0 80 99.5 81 59.5 82 37.5 83 24.5 84 16.5 85 10.5 86 3.5 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020723656880442913 2 0.009441301540065106 3 0.001416195231009766 4 0.0 5 2.3603253850162768E-4 6 0.0 7 0.0 8 0.0 9 1.4161952310097663E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0021006895926644863 26-27 0.009748143840117223 28-29 3.304455539022787E-4 30-31 0.0 32-33 2.124292846514649E-4 34-35 0.009771747093967385 36-37 0.001014939915556999 38-39 0.012509724540586267 40-41 0.012179278986683988 42-43 0.004933080054684019 44-45 6.844943616547203E-4 46-47 7.080976155048831E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.6522277695113936E-4 66-67 8.261138847556969E-4 68-69 0.001227369200208464 70-71 1.1801626925081384E-4 72-73 3.06842300052116E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.3603253850162768E-4 82-83 0.0012509724540586266 84-85 0.006915753378097691 86-87 0.004484618231530926 88-89 0.0 90-91 0.0012745757079087895 92-93 5.900813462540691E-4 94-95 4.7206507700325535E-5 96-97 2.3603253850162767E-5 98-99 0.0 100-101 4.7206507700325535E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2118352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.06321512974574 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3453758415963 47.05675163594583 2 12.705951520274722 15.26320599180761 3 3.9217795334683268 7.066640634304259 4 1.7159316261028772 4.122574816262062 5 0.8906957685091966 2.674902577956104 6 0.5488446055347186 1.9779222969019346 7 0.3492059662411238 1.4682103152451944 8 0.26184609509858864 1.2581854672632313 9 0.20077731450222985 1.0853397930708044 >10 0.9770215578475208 10.480212522702484 >50 0.053730399317087965 2.1863369459450395 >100 0.024902756028664888 2.7239772324619036 >500 0.0027559108350100024 1.1869838465272684 >1k 0.001102364334003932 1.2016449746020987 >5k 7.874030957170942E-5 0.24711094900409183 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.24665400273420093 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.2111547089435561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2971 0.14025053437766716 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.7206507700325535E-5 14-15 4.7206507700325535E-5 0.0 0.0 0.0 4.7206507700325535E-5 16-17 7.08097615504883E-5 0.0 0.0 0.0 4.7206507700325535E-5 18-19 9.441301540065107E-5 0.0 0.0 4.7206507700325535E-5 4.7206507700325535E-5 20-21 9.441301540065107E-5 0.0 0.0 7.08097615504883E-5 4.7206507700325535E-5 22-23 9.441301540065107E-5 0.0 0.0 2.3603253850162768E-4 7.08097615504883E-5 24-25 9.441301540065107E-5 0.0 0.0 4.012553154527671E-4 9.441301540065107E-5 26-27 9.441301540065107E-5 0.0 0.0 8.497171386058597E-4 9.441301540065107E-5 28-29 9.441301540065107E-5 0.0 0.0 0.0024783416542670904 9.441301540065107E-5 30-31 9.441301540065107E-5 0.0 0.0 0.00878041043226055 9.441301540065107E-5 32-33 9.441301540065107E-5 0.0 0.0 0.020393211326540633 9.441301540065107E-5 34-35 9.441301540065107E-5 0.0 0.0 0.038921765598918405 9.441301540065107E-5 36-37 9.441301540065107E-5 0.0 0.0 0.06984202814263163 9.441301540065107E-5 38-39 1.416195231009766E-4 0.0 0.0 0.11834671480471612 9.441301540065107E-5 40-41 1.416195231009766E-4 0.0 0.0 0.17393237762184943 9.441301540065107E-5 42-43 1.416195231009766E-4 0.0 0.0 0.23282249597800553 9.441301540065107E-5 44-45 1.416195231009766E-4 0.0 0.0 0.31061882066814206 9.441301540065107E-5 46-47 1.416195231009766E-4 0.0 0.0 0.400665234106513 9.441301540065107E-5 48-49 1.416195231009766E-4 0.0 0.0 0.5004125848773009 9.441301540065107E-5 50-51 1.416195231009766E-4 0.0 0.0 0.606438401172232 9.441301540065107E-5 52-53 1.416195231009766E-4 0.0 0.0 0.7106703701745507 9.441301540065107E-5 54-55 1.416195231009766E-4 0.0 0.0 0.8274592702251562 9.441301540065107E-5 56-57 1.416195231009766E-4 0.0 0.0 0.9647121913638526 9.441301540065107E-5 58-59 1.416195231009766E-4 0.0 0.0 1.1124685604658717 9.441301540065107E-5 60-61 1.6522277695113936E-4 0.0 0.0 1.271318458877467 9.441301540065107E-5 62-63 1.8882603080130214E-4 0.0 0.0 1.4326703021971796 9.441301540065107E-5 64-65 1.8882603080130214E-4 0.0 0.0 1.6091990377425471 9.441301540065107E-5 66-67 1.8882603080130214E-4 0.0 0.0 1.8111720809383898 9.441301540065107E-5 68-69 1.8882603080130214E-4 0.0 0.0 2.0365123454458938 9.441301540065107E-5 70-71 2.1242928465146492E-4 0.0 0.0 2.275046828855639 9.441301540065107E-5 72-73 2.3603253850162768E-4 0.0 0.0 2.5189628541432207 1.416195231009766E-4 74-75 2.3603253850162768E-4 0.0 0.0 2.770479127170555 1.416195231009766E-4 76-77 2.3603253850162768E-4 0.0 0.0 3.0416569106550755 1.416195231009766E-4 78-79 2.3603253850162768E-4 0.0 0.0 3.3476494935685857 1.416195231009766E-4 80-81 3.3044555390227876E-4 0.0 0.0 3.673563222731633 1.416195231009766E-4 82-83 3.776520616026043E-4 0.0 0.0 4.004952906787918 1.416195231009766E-4 84-85 4.012553154527671E-4 0.0 0.0 4.34170052946819 1.416195231009766E-4 86-87 4.2485856930292985E-4 0.0 0.0 4.6883615187655305 1.416195231009766E-4 88-89 4.2485856930292985E-4 0.0 0.0 5.066296819414337 1.416195231009766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2715 0.0 53.376194 1 GTATCAA 4910 0.0 42.38481 1 ATCAACG 6555 0.0 31.519575 3 TATCAAC 6680 0.0 31.074903 2 TCAACGC 6670 0.0 30.833717 4 CAACGCA 6940 0.0 29.702576 5 AACGCAG 7185 0.0 28.755856 6 GTGTAAG 910 0.0 26.62848 1 ACGCAGA 8440 0.0 24.42368 7 CGCAGAG 8420 0.0 24.312466 8 TGGTATC 1540 0.0 23.750612 2 GTGGTAT 1695 0.0 22.425282 1 GTACATG 6025 0.0 21.84441 1 TACATGG 5810 0.0 21.829308 2 TAAGGTG 1160 0.0 21.7011 5 ACATGGG 5905 0.0 20.591305 3 GCAGAGT 9645 0.0 20.53514 9 GTAAGGT 1150 0.0 20.237743 4 GGACCGA 540 0.0 20.230093 6 AGAGTAC 8765 0.0 20.158342 10-11 >>END_MODULE