FastQCFastQC Report
Thu 26 May 2016
SRR938627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059931
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148330.48474949271731943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112070.36625008864578973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.2629471056700298No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG34820.11379341560316229No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG32490.10617886481754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.055.0642471
GTATCAA130600.044.9671551
TATCAAC174750.033.36092
ATCAACG175700.033.04523
TCAACGC179000.032.489124
CAACGCA180250.032.0791475
AACGCAG185300.031.2844966
TGGTATC28900.029.7814082
GTGGTAT30550.028.951651
ACGCAGA211250.027.3749187
CGCAGAG216050.026.6567578
GCAGAGT226300.024.692649
GTACATG153750.024.6808091
TACATGG155000.024.0211942
AGTACTT132250.022.417712-13
GAGTACT127550.022.3683912-13
ACATGGG160700.022.0743273
AGAGTAC214150.021.67774810-11
TAAGGTG16650.021.419615
GTACTTT135650.020.75030714-15
Produced by FastQC (version 0.11.5)