##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938627_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059931 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21485713239939 31.0 31.0 34.0 28.0 34.0 2 31.254101154568517 31.0 31.0 34.0 28.0 34.0 3 31.28899442503769 31.0 31.0 34.0 28.0 34.0 4 34.954445704821445 37.0 35.0 37.0 32.0 37.0 5 34.93721623134639 37.0 35.0 37.0 32.0 37.0 6 34.96364329783907 37.0 35.0 37.0 32.0 37.0 7 34.88231074491549 37.0 35.0 37.0 32.0 37.0 8 34.853225121742945 37.0 35.0 37.0 32.0 37.0 9 36.34991148493218 39.0 35.0 39.0 32.0 39.0 10-11 36.18506250631142 39.0 35.0 39.0 32.0 39.0 12-13 36.045059839584624 38.0 35.0 39.0 30.0 39.0 14-15 36.99086123183824 39.0 36.0 41.0 31.0 41.0 16-17 36.83550102927157 39.0 36.0 41.0 31.0 41.0 18-19 37.01927739547068 39.0 36.0 41.0 31.0 41.0 20-21 37.0060078152089 39.0 36.0 41.0 31.0 41.0 22-23 36.91993969798665 39.0 36.0 41.0 30.5 41.0 24-25 36.78257238480214 39.0 36.0 41.0 30.0 41.0 26-27 36.563286067561656 39.0 36.0 41.0 30.0 41.0 28-29 36.34966850559702 39.0 35.0 40.5 29.5 41.0 30-31 36.06574265890309 38.5 35.0 40.0 29.0 41.0 32-33 35.771324582155614 38.0 35.0 40.0 27.5 41.0 34-35 35.49982728368712 38.0 34.5 40.0 27.0 41.0 36-37 35.213091896516616 38.0 34.0 40.0 26.0 41.0 38-39 35.053795657483775 38.0 34.0 40.0 25.0 41.0 40-41 34.91767918949806 38.0 34.0 40.0 25.0 41.0 42-43 34.58222489330642 38.0 33.5 40.0 24.0 41.0 44-45 34.17248836656775 38.0 33.0 40.0 23.5 41.0 46-47 33.700926752923515 38.0 33.0 40.0 21.0 41.0 48-49 33.276152632199874 37.5 32.5 40.0 18.5 41.0 50-51 32.54182332869597 36.5 31.5 39.5 15.0 40.5 52-53 32.544541200438836 37.0 31.0 39.0 15.0 40.0 54-55 32.634715782806865 37.0 31.5 40.0 13.5 41.0 56-57 32.313121439666446 36.5 31.0 40.0 8.5 41.0 58-59 31.9367951434199 36.0 31.0 39.0 7.5 41.0 60-61 31.458004608600653 35.5 30.0 39.0 4.5 40.5 62-63 30.882842619653843 35.0 29.0 39.0 2.0 40.0 64-65 30.369378753965368 35.0 28.5 38.0 2.0 40.0 66-67 29.83153721440124 34.5 28.0 38.0 2.0 40.0 68-69 29.23809491129048 34.0 27.5 37.0 2.0 39.5 70-71 28.60941423188954 34.0 26.0 36.5 2.0 39.0 72-73 27.97831241946305 33.5 26.0 36.0 2.0 39.0 74-75 27.307803672697197 33.0 25.0 35.5 2.0 38.5 76-77 26.562261371253143 32.5 23.0 35.0 2.0 37.5 78-79 25.775517323756645 32.0 20.5 35.0 2.0 37.0 80-81 25.08502413943321 31.0 18.5 35.0 2.0 36.0 82-83 24.35430913311444 31.0 13.5 34.0 2.0 36.0 84-85 23.647119330468563 30.5 7.0 34.0 2.0 35.0 86-87 22.882159597716416 30.0 2.0 34.0 2.0 35.0 88-89 22.06568138301158 29.0 2.0 34.0 2.0 35.0 90-91 21.144092955037223 29.0 2.0 34.0 2.0 35.0 92-93 20.18234447116618 27.0 2.0 33.5 2.0 35.0 94-95 19.175059829780476 25.5 2.0 33.0 2.0 35.0 96-97 17.91227710690208 24.0 2.0 32.5 2.0 34.0 98-99 16.383717149177546 10.0 2.0 32.0 2.0 34.0 100-101 12.890071214024108 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.57407165880241 1101 2 0.47702604530905646 1101 3 0.5860116339751507 1101 4 0.460972746488288 1101 5 0.4955097857813371 1101 6 0.5930227126250571 1101 7 0.5340674856032166 1101 8 0.6099612084573565 1101 9 0.7367443943799756 1101 10-11 0.7157843575382401 1101 12-13 0.8037828542922867 1101 14-15 0.9636747127643659 1101 16-17 0.8486214376275854 1101 18-19 0.884855957880923 1101 20-21 0.9002060233858273 1101 22-23 1.072591879286918 1101 24-25 1.148198227408237 1101 26-27 1.0589668735418911 1101 28-29 1.0869024402511371 1101 30-31 0.9994664284983159 1101 32-33 1.0099201356335286 1101 34-35 1.0520423880508858 1101 36-37 1.04344425838773 1101 38-39 1.1491706080547814 1101 40-41 1.2304397954361903 1101 42-43 1.3301523798594914 1101 44-45 1.4174877193212936 1101 46-47 1.4228026721452522 1101 48-49 1.1862874644882666 1101 50-51 1.3559067370624618 1101 52-53 1.4486734826754883 1101 54-55 1.388780031432951 1101 56-57 1.4503338102076881 1101 58-59 1.564014364376785 1101 60-61 1.61799130504021 1101 62-63 1.4136833773263326 1101 64-65 1.3903126091825833 1101 66-67 1.6286576719097887 1101 68-69 1.5481556953295517 1101 70-71 1.5717317832322912 1101 72-73 1.8012590212339212 1101 74-75 1.5171449131193029 1101 76-77 1.6249622182687737 1101 78-79 1.8478166586824827 1101 80-81 2.0201524701143434 1101 82-83 1.9843670607816861 1101 84-85 2.1022786605202057 1101 86-87 2.1934727960285585 1101 88-89 2.1270504020431034 1101 90-91 2.0789530004997445 1101 92-93 1.9720977441802106 1101 94-95 1.98547355501195 1101 96-97 2.0295510572418145 1101 98-99 2.0425443349561796 1101 100-101 1.8688971599737823 1102 1 0.4406500951040293 1102 2 0.4318274530516426 1102 3 0.4021085000720177 1102 4 0.316106620047762 1102 5 0.3143568268602124 1102 6 0.41977203980691513 1102 7 0.4196132323081372 1102 8 0.4180182673794661 1102 9 0.618932704595963 1102 10-11 0.674642390444717 1102 12-13 0.6614579102372389 1102 14-15 0.7924628268033516 1102 16-17 0.8355979703221053 1102 18-19 0.7609380121204197 1102 20-21 0.6851659500412595 1102 22-23 0.83586944018559 1102 24-25 0.840599941739022 1102 26-27 0.8228916214199273 1102 28-29 0.6643810234390628 1102 30-31 0.8114971809702425 1102 32-33 0.8348245116624753 1102 34-35 0.6558381421494133 1102 36-37 0.9940806014116177 1102 38-39 0.6992452834754843 1102 40-41 0.9381853370917668 1102 42-43 0.9836319340743458 1102 44-45 0.8187803681053012 1102 46-47 0.8879322081225425 1102 48-49 1.033818739441685 1102 50-51 1.0504681237086189 1102 52-53 0.8025757047005513 1102 54-55 0.7512744862284464 1102 56-57 0.8690642787651512 1102 58-59 0.9040555467622617 1102 60-61 0.8337423968293685 1102 62-63 0.9736015301078105 1102 64-65 0.9751974907370169 1102 66-67 0.6593147663212093 1102 68-69 0.9225385756812905 1102 70-71 1.078355714856226 1102 72-73 1.0790402493611744 1102 74-75 1.0239499880904646 1102 76-77 1.1858432650062234 1102 78-79 1.1438531929543103 1102 80-81 1.3086751290522294 1102 82-83 1.4079503226435008 1102 84-85 1.544002011499611 1102 86-87 1.7819477662076828 1102 88-89 1.9505397651085907 1102 90-91 1.9022944914450406 1102 92-93 2.059373841144886 1102 94-95 1.8454013194812369 1102 96-97 1.9668180354257458 1102 98-99 1.947084625179862 1102 100-101 1.5915171581993182 1103 1 0.4588190135770134 1103 2 0.4294672991808284 1103 3 0.41464256001752275 1103 4 0.3172029024459633 1103 5 0.3783348399279234 1103 6 0.46601696027870787 1103 7 0.35224171726518705 1103 8 0.42876423911324935 1103 9 0.5563392459911753 1103 10-11 0.6896610585947798 1103 12-13 0.7336989367969267 1103 14-15 0.8597889678704504 1103 16-17 0.793474892528728 1103 18-19 0.8782943239350942 1103 20-21 0.7608193352934123 1103 22-23 0.8740291895798151 1103 24-25 0.8864776276426838 1103 26-27 0.8491328648318657 1103 28-29 0.9031859082716664 1103 30-31 0.8719095066634637 1103 32-33 0.9402452285948613 1103 34-35 0.9522763439728621 1103 36-37 0.8689302190916735 1103 38-39 0.8777943533679036 1103 40-41 1.0183511347780438 1103 42-43 1.06183957953035 1103 44-45 1.072324134545994 1103 46-47 0.7462629879420817 1103 48-49 0.759752146944777 1103 50-51 0.9126670055052912 1103 52-53 0.988671114975574 1103 54-55 0.9999813868441194 1103 56-57 1.0326167192957811 1103 58-59 1.0048665034578086 1103 60-61 1.1025299334821064 1103 62-63 1.265430914530274 1103 64-65 1.1924767257199989 1103 66-67 1.133615771384239 1103 68-69 1.3432797860939694 1103 70-71 1.4277426358371486 1103 72-73 1.5039108451391527 1103 74-75 1.5571085825107644 1103 76-77 1.4090226489572686 1103 78-79 1.3068360427699872 1103 80-81 1.4607814243672763 1103 82-83 1.578895539165753 1103 84-85 1.7167836365715026 1103 86-87 1.8439415812044295 1103 88-89 1.9459145659345154 1103 90-91 1.6541153432029603 1103 92-93 1.5352464357348907 1103 94-95 1.6631936928819506 1103 96-97 2.028304133310968 1103 98-99 1.9947837521138538 1103 100-101 1.5980856599388495 1104 1 0.33476547094026543 1104 2 0.2572372099967559 1104 3 0.3401130947553135 1104 4 0.17806159760240803 1104 5 0.2790747466928707 1104 6 0.34586248216157855 1104 7 0.294072539380835 1104 8 0.3182792308547988 1104 9 0.5090485710162795 1104 10-11 0.5215722653117112 1104 12-13 0.5072766462337341 1104 14-15 0.6332907215049275 1104 16-17 0.7398001295734176 1104 18-19 0.6630129442960353 1104 20-21 0.6125327059291195 1104 22-23 0.5943083380521159 1104 24-25 0.5906777878932203 1104 26-27 0.6599476967700113 1104 28-29 0.6702320335085261 1104 30-31 0.6743536067699765 1104 32-33 0.5772182439966187 1104 34-35 0.5613708971537719 1104 36-37 0.7237696823954636 1104 38-39 0.8599240042774952 1104 40-41 0.748401977658304 1104 42-43 0.7712774484665132 1104 44-45 0.8450928224679188 1104 46-47 0.8919831607300708 1104 48-49 0.5336436281801724 1104 50-51 0.5893110266490638 1104 52-53 0.7082102672574067 1104 54-55 0.6808948059484479 1104 56-57 0.8692057191518003 1104 58-59 0.728976082361978 1104 60-61 0.7703240137808613 1104 62-63 0.967707196467483 1104 64-65 0.9327487380356771 1104 66-67 0.9772218801310366 1104 68-69 1.1621862863457046 1104 70-71 1.302051152992096 1104 72-73 1.3499585500778544 1104 74-75 1.178888704826992 1104 76-77 1.1404278413607507 1104 78-79 1.2409998560173037 1104 80-81 1.3136458132466764 1104 82-83 1.4473942478612223 1104 84-85 1.484588872704066 1104 86-87 1.6694969807621298 1104 88-89 1.6209461890345587 1104 90-91 1.6676576088343182 1104 92-93 1.640836179164129 1104 94-95 1.881641742019486 1104 96-97 1.9323089658661488 1104 98-99 2.0887814328629517 1104 100-101 1.9513027207186955 1105 1 0.3855915458256547 1105 2 0.2762205122887593 1105 3 0.323712846508581 1105 4 0.2785843233280474 1105 5 0.33858756929536327 1105 6 0.46940760662739933 1105 7 0.403342847328247 1105 8 0.3749116445487317 1105 9 0.4889767506391749 1105 10-11 0.564405472937878 1105 12-13 0.5493010696091645 1105 14-15 0.592635284258499 1105 16-17 0.6040652387928631 1105 18-19 0.563641983562924 1105 20-21 0.5558447545279108 1105 22-23 0.5724455708352423 1105 24-25 0.5963529654119739 1105 26-27 0.6406003314337383 1105 28-29 0.760542061877338 1105 30-31 0.7558263220226351 1105 32-33 0.7870153682656706 1105 34-35 0.8891768388598322 1105 36-37 0.8086378021047622 1105 38-39 0.7464481440907065 1105 40-41 0.7482139675727808 1105 42-43 0.8506586105167315 1105 44-45 0.8641634956950597 1105 46-47 0.8495579638334263 1105 48-49 0.8234749414408427 1105 50-51 0.8876949370553575 1105 52-53 0.8015293485347073 1105 54-55 0.8717403038227545 1105 56-57 0.9241061849867762 1105 58-59 0.9330962984755011 1105 60-61 0.9040822027082918 1105 62-63 0.8445795225681181 1105 64-65 1.0049243676701813 1105 66-67 1.1728168746260401 1105 68-69 1.0051444249692914 1105 70-71 1.0455017679083163 1105 72-73 0.9853993899380491 1105 74-75 1.1152593266699569 1105 76-77 1.222939200893407 1105 78-79 1.3222732858236732 1105 80-81 1.3889938574121068 1105 82-83 1.549883029401684 1105 84-85 1.52221464673136 1105 86-87 1.6880839672336911 1105 88-89 1.5264702557414793 1105 90-91 1.5635294947238911 1105 92-93 1.52367138803573 1105 94-95 1.581291933128984 1105 96-97 1.847513637788289 1105 98-99 2.0626804410822217 1105 100-101 1.5582424902587118 1106 1 0.21999332972408325 1106 2 0.10394357222658357 1106 3 0.014770837734356945 1106 4 0.140524676040215 1106 5 0.1857112509828056 1106 6 -0.018629524027176103 1106 7 0.21534639188212878 1106 8 0.09153981503596498 1106 9 -0.0024756448400680142 1106 10-11 0.0759764802303593 1106 12-13 0.17042130843073977 1106 14-15 0.40104706263671375 1106 16-17 0.17755916656530957 1106 18-19 0.07813906366403245 1106 20-21 0.20491104059084364 1106 22-23 0.26331180750684524 1106 24-25 0.11136792807793938 1106 26-27 0.23239483446985076 1106 28-29 0.36611674446544384 1106 30-31 0.028905507937395214 1106 32-33 0.15520771842111003 1106 34-35 -0.057698796690296206 1106 36-37 -0.033558368614897915 1106 38-39 -0.013326616942443081 1106 40-41 0.2634442909406829 1106 42-43 -0.27723514002802574 1106 44-45 0.08878216561574703 1106 46-47 0.1042170910596738 1106 48-49 0.46821803391883066 1106 50-51 0.18961284576856485 1106 52-53 -7.030667010567981E-4 1106 54-55 0.02836128351420797 1106 56-57 -0.6974582125843298 1106 58-59 -0.37698352662110324 1106 60-61 0.22037734492625205 1106 62-63 0.14310395674691634 1106 64-65 0.04683109070106539 1106 66-67 -0.3042575610054463 1106 68-69 -0.24266274548888944 1106 70-71 -0.3542741552736466 1106 72-73 0.051857959332860304 1106 74-75 0.4182271642015536 1106 76-77 0.28537310367965674 1106 78-79 0.49840819677402237 1106 80-81 0.5599524849143585 1106 82-83 0.3016197155343434 1106 84-85 0.25733841557995873 1106 86-87 0.17779495535071632 1106 88-89 0.21520576519846912 1106 90-91 0.3202274667742131 1106 92-93 -0.06260690302443805 1106 94-95 0.20582126285965785 1106 96-97 0.2841274993182594 1106 98-99 0.737559504971351 1106 100-101 0.421423800000424 1107 1 0.1057946842619053 1107 2 -0.030926135856855552 1107 3 0.1323590413649356 1107 4 0.13351950212350516 1107 5 0.1304176271924291 1107 6 0.14730236344816916 1107 7 0.19555595961102767 1107 8 0.14047761555897864 1107 9 0.3059536617024463 1107 10-11 0.28621610528208663 1107 12-13 0.2683796097953248 1107 14-15 0.4154449520964718 1107 16-17 0.4607310325897913 1107 18-19 0.4016031575657948 1107 20-21 0.46007275561569116 1107 22-23 0.45157298184132344 1107 24-25 0.5487659460822059 1107 26-27 0.48246001055575505 1107 28-29 0.4988360434157926 1107 30-31 0.5715706876386051 1107 32-33 0.6137834954124486 1107 34-35 0.5069590439225422 1107 36-37 0.5233545143573721 1107 38-39 0.5564783267992439 1107 40-41 0.47885160632866786 1107 42-43 0.5500654050810567 1107 44-45 0.5047648683445018 1107 46-47 0.4924440705447708 1107 48-49 0.588007110447009 1107 50-51 0.6073251249833262 1107 52-53 0.7046084830235699 1107 54-55 0.6905171256615432 1107 56-57 0.7762117533915429 1107 58-59 0.6981421557694993 1107 60-61 0.5860787058912997 1107 62-63 0.6725061544564106 1107 64-65 0.760130175624802 1107 66-67 0.8112962248313629 1107 68-69 0.936320326805351 1107 70-71 1.0452285883483334 1107 72-73 0.9740400727653586 1107 74-75 0.9643559658051437 1107 76-77 0.9907145801740889 1107 78-79 0.9799897261323842 1107 80-81 1.258004332540903 1107 82-83 1.0779905222716835 1107 84-85 1.32601824333031 1107 86-87 1.4519557504788807 1107 88-89 1.3410150433005086 1107 90-91 1.2452871696757022 1107 92-93 1.4121155713771252 1107 94-95 1.4793506332727269 1107 96-97 1.4184678148950383 1107 98-99 1.4245843923944719 1107 100-101 1.1712875729426067 1108 1 0.25053986652061866 1108 2 0.17836637164938196 1108 3 0.19453274249626062 1108 4 0.11116861543415268 1108 5 0.20750959153114223 1108 6 0.31461362171597074 1108 7 0.26258665162238515 1108 8 0.4494943604905046 1108 9 0.28699044462602075 1108 10-11 0.4059593445882328 1108 12-13 0.3943074916919258 1108 14-15 0.45532195441160894 1108 16-17 0.5848418144729592 1108 18-19 0.6650618630868266 1108 20-21 0.37292601173081863 1108 22-23 0.3807495780696115 1108 24-25 0.5501666293766405 1108 26-27 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70-71 -2.0551432620511143 2316 72-73 -2.054854259316656 2316 74-75 -2.0164222699655667 2316 76-77 -2.2369308383590116 2316 78-79 -2.3670775634464647 2316 80-81 -2.4960848057692324 2316 82-83 -2.6404951631560962 2316 84-85 -2.8368247363806205 2316 86-87 -2.7865622710241915 2316 88-89 -3.1228068251051226 2316 90-91 -3.115630365104522 2316 92-93 -3.205705725423904 2316 94-95 -3.485007442197343 2316 96-97 -3.649739477756114 2316 98-99 -3.3640549978891343 2316 100-101 -2.9996785301453155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10570.0 3 3315.0 4 3484.0 5 3946.0 6 4930.0 7 6151.0 8 7736.0 9 10041.0 10 12777.0 11 16344.0 12 20749.0 13 24704.0 14 28971.0 15 34404.0 16 41283.0 17 48746.0 18 44098.0 19 39137.0 20 40716.0 21 43960.0 22 48844.0 23 54001.0 24 59940.0 25 67614.0 26 75782.0 27 85698.0 28 97737.0 29 111373.0 30 127692.0 31 148590.0 32 173950.0 33 207219.0 34 250900.0 35 301515.0 36 344713.0 37 320195.0 38 132568.0 39 5538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52842013614264 18.525707480262977 15.077693079001692 22.86817930459269 2 15.708108564356419 20.184567650213943 39.67183756633472 24.435486219094912 3 19.064813166794046 24.490219917707538 29.448314627328216 26.996652288170196 4 13.108576611288376 16.652030290101273 36.18992432709749 34.049468771512856 5 14.172792258399863 37.26404456316841 32.98598825104032 15.577174927391402 6 32.168480911583245 35.39221808492021 17.369777210484358 15.069523793012193 7 29.06442234203983 32.853223863013035 19.819560954975557 18.262792839971574 8 27.221458563861 33.69668579535518 19.56784745865212 19.5140081821317 9 27.507446371740656 16.423629906825557 19.575491434384322 36.49343228704946 10-11 25.485459511226654 26.30275200260529 26.54578829194828 21.66600019421977 12-13 26.54755258074616 23.772848887729918 27.06021127940692 22.619387252117004 14-15 23.347178929196556 24.95386299643146 25.943665374291193 25.75529270008079 16-17 22.501105356627686 28.236243111442015 26.355690277847017 22.90696125408328 18-19 22.54512970268788 26.29748865780868 29.36736276786247 21.790018871640967 20-21 23.333610734475933 25.43919985520641 29.447559166966002 21.77963024335165 22-23 23.02512192865486 25.235921608676932 29.28474733741293 22.454209125255275 24-25 22.741562791105245 25.524157014792436 29.32053726359679 22.413742930505524 26-27 22.149155039670944 26.032197482163237 29.310623612653174 22.50802386551264 28-29 22.274371370967476 26.170707335591214 29.386866392747397 22.16805490069391 30-31 22.332467374476845 25.80071762088923 29.142442383322837 22.72437262131109 32-33 22.28905804378849 25.85627515505215 29.00534968128011 22.849317119879256 34-35 23.03274595601063 25.98399899020849 28.67852038075157 22.304734673029312 36-37 23.02340496735736 25.531492262240395 28.823843210702254 22.62125955969999 38-39 22.4345306055066 25.945241327343293 28.71534267689997 22.90488539025014 40-41 22.76162726548293 25.746102449888642 28.708739094011154 22.783531190617275 42-43 22.76287255762986 25.74670077652729 28.480393286627603 23.010033379215248 44-45 22.453778980364422 26.093791517537174 28.494091416122032 22.958338085976372 46-47 22.742847075911957 26.477307863526217 27.822699523258237 22.95714553730359 48-49 22.842896582514257 26.172512711433704 27.91508301447847 23.06950769157357 50-51 23.11347039999948 26.572934611768094 27.40521146172523 22.908383526507194 52-53 23.149649328456274 26.545534353097327 27.27256682549309 23.032249492953312 54-55 22.615496922876545 26.594749729089916 27.572983936269985 23.21676941176355 56-57 22.65466860032038 26.669108247673716 27.643142180746942 23.03308097125896 58-59 22.773866902621197 26.18634404438987 27.592297897606567 23.447491155382366 60-61 22.680542282661087 26.41371648835905 27.799807105140445 23.105934123839415 62-63 22.447971387020583 26.65660649684982 27.733221161459355 23.162200954670244 64-65 22.226082494527574 26.344174143674575 27.707676431103806 23.72206693069404 66-67 22.330957495573394 26.431152862812326 27.632575587347784 23.6053140542665 68-69 22.468114522161112 26.251555563545026 27.788938523475014 23.491391390818844 70-71 22.449902263667926 26.475292297749377 27.45044901177527 23.624356426807427 72-73 22.54079999686182 26.487664098536186 27.31231854341449 23.659217361187498 74-75 22.34444047081222 26.71814554434149 27.24271948125139 23.694694503594903 76-77 22.533813738621497 26.33933948903684 27.291346085484136 23.83550068685753 78-79 22.659433447982398 26.447558837717324 27.363770895347756 23.529236818952523 80-81 22.561854538864647 26.820739860503018 27.06611701921658 23.551288581415754 82-83 22.586281305514238 26.581533196644358 27.05111488884121 23.781070609000196 84-85 22.58944826435569 26.288776792646118 27.14661862822109 23.9751563147771 86-87 22.674601221299095 26.381773142205876 26.96256515300231 23.98106048349272 88-89 22.56782223930961 26.269698784142008 27.044891822296197 24.117587154252185 90-91 22.487739669398522 26.23123926679611 27.11251846691354 24.168502596891837 92-93 22.58050069196851 25.709322884257457 27.246168021100832 24.464008402673205 94-95 22.414272827529473 25.84924494304581 27.239333126478865 24.497149102945844 96-97 22.586444690486463 25.673316612429716 27.148037538826408 24.592201158257417 98-99 22.51453013245466 25.697413669111786 26.83870735757528 24.949348840858274 100-101 22.943118545954032 25.09575085645139 27.1462303874676 24.814900210126975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1275.0 1 869.0 2 581.5 3 716.5 4 615.5 5 621.0 6 1045.5 7 2296.0 8 3751.0 9 4295.5 10 3757.5 11 3148.5 12 3293.0 13 3587.0 14 3900.5 15 4332.0 16 4721.5 17 5071.0 18 5513.0 19 6020.5 20 6632.0 21 7604.5 22 8796.0 23 9997.5 24 11568.0 25 13827.5 26 16874.0 27 20744.5 28 24257.5 29 27707.5 30 32290.0 31 37217.5 32 43048.0 33 46569.0 34 47988.0 35 50543.0 36 54925.0 37 61061.0 38 65567.5 39 69040.5 40 73764.5 41 82128.5 42 91103.5 43 97631.0 44 101394.0 45 105585.5 46 108481.5 47 111183.0 48 116061.5 49 118292.5 50 119621.5 51 117241.0 52 114010.0 53 113532.5 54 113156.5 55 107256.5 56 96128.0 57 87016.0 58 80448.0 59 72284.0 60 60699.5 61 50679.5 62 42831.5 63 34273.0 64 27610.5 65 22869.0 66 17944.0 67 14212.5 68 11101.5 69 8202.5 70 6450.5 71 5150.0 72 4302.5 73 3544.5 74 2663.0 75 2097.0 76 1485.5 77 920.0 78 592.0 79 378.0 80 214.0 81 130.0 82 77.5 83 46.0 84 30.0 85 19.0 86 14.0 87 9.0 88 6.0 89 7.0 90 5.0 91 3.0 92 2.5 93 1.5 94 1.0 95 1.5 96 2.5 97 1.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006503414619479981 2 0.10699587670440935 3 0.11487187129382982 4 0.11827064074320631 5 0.15856566700360236 6 0.09542698838633942 7 0.02735355797238565 8 0.026994072742163144 9 0.02362798376826144 10-11 0.05032793223115162 12-13 0.05420056857491231 14-15 0.02730453725917349 16-17 0.03148763811994453 18-19 0.03622630706378673 20-21 0.05831830848473381 22-23 0.03170006121053056 24-25 0.050458654133050715 26-27 0.05589995329960054 28-29 0.052582885038911 30-31 0.06696229424781147 32-33 0.04982138486129262 34-35 0.061243211039726055 36-37 0.05178221338977904 38-39 0.03148763811994453 40-41 0.03655311181853447 42-43 0.0324026914332382 44-45 0.0075165093591979685 46-47 0.013497036371081572 48-49 0.009281255034835753 50-51 0.008235479819642993 52-53 0.009853163355644293 54-55 0.010996979997261376 56-57 0.014314048257950913 58-59 0.019101737915005274 60-61 0.024559377319292496 62-63 0.011127701899160472 64-65 0.008938110042350627 66-67 0.01008192668396771 68-69 0.011389145702958662 70-71 0.03057258480665087 72-73 0.027173815357274398 74-75 0.0286607769913766 76-77 0.02315411687387722 78-79 0.005196195600489031 80-81 0.0049510920344282274 82-83 0.00609490867604531 84-85 0.005212535838226417 86-87 0.013137551140859058 88-89 0.03740280418087859 90-91 0.035360274463705224 92-93 0.029559490066932884 94-95 0.046406275174178765 96-97 0.04197807074734691 98-99 0.025196646591050583 100-101 0.07372715267108965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.10285948982134 #Duplication Level Percentage of deduplicated Percentage of total 1 69.93000728490503 30.14183278123444 2 14.411809866647152 12.42380431352226 3 5.868107046916026 7.587965805433557 4 3.0885287962475005 5.324976909396922 5 1.7798678159272052 3.8358696190182755 6 1.118219808667946 2.891908277504962 7 0.7743752307932229 2.336445073569478 8 0.524170488412786 1.8074597528613872 9 0.3999107599292105 1.551356756432679 >10 1.8773287412186912 14.365064608212661 >50 0.1270781783458661 3.7918440234359254 >100 0.08763216299140418 7.512274830860901 >500 0.00930344657523394 2.791160193858437 >1k 0.003431599146511155 2.5176549596013715 >5k 7.625775881135899E-5 0.26446618367738045 >10k+ 1.5251551762271798E-4 0.8559159113794417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14833 0.48474949271731943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11207 0.36625008864578973 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8046 0.2629471056700298 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3482 0.11379341560316229 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3249 0.10617886481754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.536095094954755E-5 7 0.0 0.0 0.0 0.0 6.536095094954755E-5 8 0.0 0.0 0.0 0.0 6.536095094954755E-5 9 0.0 0.0 0.0 0.0 6.536095094954755E-5 10-11 0.0 0.0 0.0 1.6340237737386888E-5 6.536095094954755E-5 12-13 0.0 0.0 0.0 3.2680475474773776E-5 1.7974261511125576E-4 14-15 0.0 0.0 0.0 3.2680475474773776E-5 2.451035660608033E-4 16-17 0.0 0.0 0.0 3.2680475474773776E-5 2.614438037981902E-4 18-19 0.0 0.0 0.0 8.170118868693444E-5 2.614438037981902E-4 20-21 0.0 0.0 0.0 1.47062139636482E-4 3.1046451701035086E-4 22-23 0.0 0.0 0.0 5.228876075963804E-4 3.5948523022251156E-4 24-25 0.0 0.0 0.0 0.001176497117091856 3.5948523022251156E-4 26-27 0.0 0.0 0.0 0.002614438037981902 3.5948523022251156E-4 28-29 0.0 0.0 0.0 0.00656877557042953 3.5948523022251156E-4 30-31 0.0 0.0 0.0 0.0186932319715706 3.5948523022251156E-4 32-33 0.0 0.0 0.0 0.04207611217377123 3.5948523022251156E-4 34-35 0.0 0.0 0.0 0.07657035403739496 3.5948523022251156E-4 36-37 0.0 0.0 0.0 0.1326010292388946 3.7582546795989847E-4 38-39 0.0 3.2680475474773776E-5 0.0 0.2182075347450645 4.248461811720591E-4 40-41 0.0 3.2680475474773776E-5 0.0 0.31304627457285805 4.248461811720591E-4 42-43 0.0 3.2680475474773776E-5 0.0 0.40796671558933845 4.248461811720591E-4 44-45 0.0 3.2680475474773776E-5 0.0 0.5212699240603791 4.248461811720591E-4 46-47 0.0 3.2680475474773776E-5 0.0 0.6547206456616179 4.248461811720591E-4 48-49 0.0 3.2680475474773776E-5 0.0 0.8007370100829071 4.5752665664683287E-4 50-51 0.0 3.2680475474773776E-5 0.0 0.9597602037431563 4.902071321216066E-4 52-53 0.0 3.2680475474773776E-5 0.0 1.1170513322032425 5.228876075963804E-4 54-55 0.0 3.2680475474773776E-5 0.0 1.2903722338837054 5.228876075963804E-4 56-57 0.0 3.2680475474773776E-5 0.0 1.4858014772228523 5.719083208085411E-4 58-59 0.0 3.2680475474773776E-5 0.0 1.7013292129789854 5.88248558545928E-4 60-61 0.0 3.2680475474773776E-5 0.0 1.936759358299256 6.04588796283315E-4 62-63 0.0 3.2680475474773776E-5 0.0 2.169362642490958 6.209290340207018E-4 64-65 0.0 3.2680475474773776E-5 0.0 2.4110674391023847 6.536095094954755E-4 66-67 0.0 3.2680475474773776E-5 0.0 2.688639057547376 6.536095094954755E-4 68-69 0.0 3.2680475474773776E-5 0.0 2.9982702224331206 6.699497472328624E-4 70-71 0.0 3.2680475474773776E-5 0.0 3.3284410661547597 6.862899849702493E-4 72-73 0.0 3.2680475474773776E-5 0.0 3.6643963540354343 7.026302227076363E-4 74-75 0.0 3.2680475474773776E-5 0.0 4.01497615469107 7.3531069818241E-4 76-77 0.0 3.2680475474773776E-5 0.0 4.388513989367734 7.516509359197969E-4 78-79 0.0 3.2680475474773776E-5 0.0 4.800075557259298 7.516509359197969E-4 80-81 0.0 3.2680475474773776E-5 0.0 5.237487381251407 8.006716491319576E-4 82-83 0.0 3.2680475474773776E-5 0.0 5.68351051053112 8.170118868693444E-4 84-85 0.0 3.2680475474773776E-5 0.0 6.142033921679934 8.170118868693444E-4 86-87 0.0 3.2680475474773776E-5 0.0 6.608678430984227 8.333521246067313E-4 88-89 0.0 3.2680475474773776E-5 0.0 7.108346560755782 8.496923623441182E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6805 0.0 55.064247 1 GTATCAA 13060 0.0 44.967155 1 TATCAAC 17475 0.0 33.3609 2 ATCAACG 17570 0.0 33.0452 3 TCAACGC 17900 0.0 32.48912 4 CAACGCA 18025 0.0 32.079147 5 AACGCAG 18530 0.0 31.284496 6 TGGTATC 2890 0.0 29.781408 2 GTGGTAT 3055 0.0 28.95165 1 ACGCAGA 21125 0.0 27.374918 7 CGCAGAG 21605 0.0 26.656757 8 GCAGAGT 22630 0.0 24.69264 9 GTACATG 15375 0.0 24.680809 1 TACATGG 15500 0.0 24.021194 2 AGTACTT 13225 0.0 22.4177 12-13 GAGTACT 12755 0.0 22.36839 12-13 ACATGGG 16070 0.0 22.074327 3 AGAGTAC 21415 0.0 21.677748 10-11 TAAGGTG 1665 0.0 21.41961 5 GTACTTT 13565 0.0 20.750307 14-15 >>END_MODULE