FastQCFastQC Report
Thu 26 May 2016
SRR938627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059931
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141300.4617751184585535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115260.3766751603222426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83630.2733068163955331No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG36530.11938177690934862No Hit
ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT33760.11032928520283626No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC30830.10075390588872755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71150.056.1760251
GTATCAA131450.044.0368841
ATCAACG177650.032.3300483
TATCAAC177800.032.289782
TCAACGC180700.031.8369334
CAACGCA183300.031.3853445
AACGCAG188050.030.5528286
ACGCAGA216350.026.4685297
CGCAGAG219600.026.0768058
GTACATG150950.024.9672071
TACATGG151600.024.672062
TGGTATC30300.024.4687542
GCAGAGT234100.024.055969
GGACCGA15600.023.437196
GTGGTAT32250.023.4313491
ACATGGG156900.022.889913
AGGACCG18400.022.7200935
GTGTAAG11300.022.7114661
AGTACTT137300.022.41010312-13
GAGTACT134150.022.36999112-13
Produced by FastQC (version 0.11.5)