##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059931 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.813453963504404 31.0 31.0 34.0 30.0 34.0 2 32.0285313623085 31.0 31.0 34.0 30.0 34.0 3 32.08263192862846 33.0 31.0 34.0 30.0 34.0 4 35.763225379918694 37.0 35.0 37.0 35.0 37.0 5 35.66173616333179 37.0 35.0 37.0 33.0 37.0 6 35.66671274613709 37.0 35.0 37.0 33.0 37.0 7 35.606942117322255 37.0 35.0 37.0 33.0 37.0 8 35.586622378086304 37.0 35.0 37.0 33.0 37.0 9 37.15836762332223 39.0 37.0 39.0 33.0 39.0 10-11 37.05953189794149 39.0 37.0 39.0 33.0 39.0 12-13 36.9482836377683 39.0 37.0 39.0 33.0 39.0 14-15 37.96794568243532 40.0 37.0 41.0 33.0 41.0 16-17 37.89789851470507 40.0 37.0 41.0 33.0 41.0 18-19 38.15577704203134 40.0 37.0 41.0 33.0 41.0 20-21 38.12931909248934 40.0 37.0 41.0 33.0 41.0 22-23 38.02330461046344 40.0 37.0 41.0 33.0 41.0 24-25 37.8984045718678 40.0 37.0 41.0 32.5 41.0 26-27 37.69919272689482 40.0 37.0 41.0 32.0 41.0 28-29 37.575733733865235 39.5 36.5 41.0 32.0 41.0 30-31 37.297439713509874 39.0 36.0 41.0 31.5 41.0 32-33 37.0744593587241 39.0 35.5 41.0 31.0 41.0 34-35 36.95539703346252 39.0 35.0 41.0 31.0 41.0 36-37 36.893704139080256 39.0 35.0 41.0 31.0 41.0 38-39 36.72004532128339 39.0 35.0 41.0 30.0 41.0 40-41 36.5235078830209 39.0 35.0 41.0 30.0 41.0 42-43 36.27392447738201 39.0 35.0 40.0 30.0 41.0 44-45 35.91319281382489 38.5 35.0 40.0 29.0 41.0 46-47 35.45458116539229 38.0 34.5 40.0 27.5 41.0 48-49 35.044640712486654 38.0 34.0 40.0 26.0 41.0 50-51 34.64639088267023 38.0 34.0 40.0 24.5 41.0 52-53 34.16256330616606 38.0 33.0 40.0 24.0 41.0 54-55 33.82347477769923 37.0 33.0 40.0 23.0 41.0 56-57 33.479349861157004 37.0 32.5 40.0 22.0 41.0 58-59 33.014180711918016 36.5 32.0 39.0 20.5 41.0 60-61 32.620225586786106 36.0 31.0 39.0 19.5 40.0 62-63 32.13407671610896 35.5 31.0 39.0 18.0 40.0 64-65 31.620158428409006 35.0 30.5 38.0 14.0 40.0 66-67 31.120083916924926 35.0 29.5 38.0 12.0 40.0 68-69 30.63840671570699 34.0 29.0 37.0 8.0 39.5 70-71 30.088864422106248 34.0 29.0 37.0 7.0 39.0 72-73 29.603966723432652 34.0 28.0 36.0 4.5 39.0 74-75 29.020623994462618 33.5 27.5 36.0 2.0 38.5 76-77 27.81786827872916 31.5 26.0 34.5 2.0 36.5 78-79 28.49659518466266 33.0 27.0 35.0 2.0 37.0 80-81 28.139630272708764 33.0 26.5 35.0 2.0 36.5 82-83 27.715136387062323 33.0 26.0 35.0 2.0 36.0 84-85 27.18729425598159 33.0 26.0 35.0 2.0 36.0 86-87 26.602302143414345 32.0 25.0 34.5 2.0 35.0 88-89 25.887261510145166 32.0 23.5 34.0 2.0 35.0 90-91 25.31365413141669 31.0 21.0 34.0 2.0 35.0 92-93 24.51892281231178 31.0 18.0 34.0 2.0 35.0 94-95 23.736824948013535 31.0 9.5 34.0 2.0 35.0 96-97 22.701801609251973 30.0 2.0 34.0 2.0 35.0 98-99 21.376955722204194 29.5 2.0 34.0 2.0 35.0 100-101 17.48248277493839 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27870121344190224 1101 2 0.3677529131578581 1101 3 0.32009762383057705 1101 4 0.2602665672613327 1101 5 0.13881310040910222 1101 6 0.2460398545502187 1101 7 0.2836633343618331 1101 8 0.2483813737041345 1101 9 0.33684496895342164 1101 10-11 0.38246977799742155 1101 12-13 0.3012885407707415 1101 14-15 0.4137648394294047 1101 16-17 0.5425661699368547 1101 18-19 0.5262970510467966 1101 20-21 0.4986944470683383 1101 22-23 0.4242182174593552 1101 24-25 0.4115325700032386 1101 26-27 0.4501467394864136 1101 28-29 0.49678242474353596 1101 30-31 0.587015982249703 1101 32-33 0.4066346265936218 1101 34-35 0.5010772188873602 1101 36-37 0.6156940788971994 1101 38-39 0.5808221817577817 1101 40-41 0.6691490371989559 1101 42-43 0.6717241833649368 1101 44-45 0.7389205395618461 1101 46-47 0.6063023140594552 1101 48-49 0.7933501821183526 1101 50-51 0.9143133558060867 1101 52-53 1.0005269858072765 1101 54-55 0.9586486561592977 1101 56-57 0.8731602160418674 1101 58-59 1.0863938131454773 1101 60-61 1.035316834708631 1101 62-63 1.2297603330781115 1101 64-65 0.9735679123137544 1101 66-67 0.8593896651594726 1101 68-69 1.100325027531131 1101 70-71 0.9577029345142947 1101 72-73 1.223771148018315 1101 74-75 1.0951970493039447 1101 76-77 0.8362041310347408 1101 78-79 1.0083515133423298 1101 80-81 1.0500494652373362 1101 82-83 1.020964089924206 1101 84-85 1.3301078309616692 1101 86-87 1.3686129171345875 1101 88-89 1.5291565104748877 1101 90-91 1.4828366641518151 1101 92-93 1.6282677002538044 1101 94-95 1.5423003734413534 1101 96-97 1.752372882985 1101 98-99 1.9190444472316344 1101 100-101 2.0906609300640895 1102 1 0.2726465894807042 1102 2 0.3423928445451949 1102 3 0.24573643138980117 1102 4 0.15560144832285516 1102 5 0.1596056456826176 1102 6 0.17708363455594167 1102 7 0.2290935929408775 1102 8 0.20720317794116738 1102 9 0.28416897648504147 1102 10-11 0.3092070289561306 1102 12-13 0.2681677736101804 1102 14-15 0.4774874954098465 1102 16-17 0.4325835695563356 1102 18-19 0.4348897767242903 1102 20-21 0.5071119442834089 1102 22-23 0.48125754651425723 1102 24-25 0.49519097554745883 1102 26-27 0.44530417400370936 1102 28-29 0.39723787087371676 1102 30-31 0.40352701350987275 1102 32-33 0.49669232136584895 1102 34-35 0.3721038950178439 1102 36-37 0.4042097139093812 1102 38-39 0.46397042848733605 1102 40-41 0.4024531657885504 1102 42-43 0.26751414087634373 1102 44-45 0.44080147293066574 1102 46-47 0.47741685087468255 1102 48-49 0.46487247630501116 1102 50-51 0.5356160598942523 1102 52-53 0.31602768080623633 1102 54-55 0.24479254559038566 1102 56-57 0.3887811803652568 1102 58-59 0.3925509617766707 1102 60-61 0.3869829838228682 1102 62-63 0.4921167738947574 1102 64-65 0.5864144259820705 1102 66-67 0.3711961449157748 1102 68-69 0.388233277809622 1102 70-71 0.43002361492320773 1102 72-73 0.4698964055622561 1102 74-75 0.6419951249947538 1102 76-77 0.5939003819835875 1102 78-79 0.7301116351889547 1102 80-81 0.7378443468903484 1102 82-83 0.9221979595404299 1102 84-85 0.8602116371900443 1102 86-87 0.6202826398250707 1102 88-89 0.7706326685600438 1102 90-91 0.8286336455889334 1102 92-93 1.1104203979341847 1102 94-95 0.9386544630912894 1102 96-97 0.9204015751053873 1102 98-99 1.0202754826322185 1102 100-101 1.3902462719937212 1103 1 0.2844011598775431 1103 2 0.22369098810138865 1103 3 0.21147259763061754 1103 4 0.10908310545116962 1103 5 0.05710901632082255 1103 6 0.15764895893861564 1103 7 0.13889012896192554 1103 8 0.17456832884204943 1103 9 0.3064074102461092 1103 10-11 0.33107516393251046 1103 12-13 0.34119253247991566 1103 14-15 0.5011339366758847 1103 16-17 0.4632739375451749 1103 18-19 0.46176849503322615 1103 20-21 0.4375020190502994 1103 22-23 0.31087331732972245 1103 24-25 0.40398504133855084 1103 26-27 0.44596250914415236 1103 28-29 0.3684739522593148 1103 30-31 0.39836027298772336 1103 32-33 0.41898402535310453 1103 34-35 0.48979668138066756 1103 36-37 0.5685904357445182 1103 38-39 0.5151413127730962 1103 40-41 0.5850660939538912 1103 42-43 0.6732719216292935 1103 44-45 0.6171387677460132 1103 46-47 0.5515434313033012 1103 48-49 0.5849387340951893 1103 50-51 0.6943870262067264 1103 52-53 0.5535484231951884 1103 54-55 0.5404978103858369 1103 56-57 0.483254865816356 1103 58-59 0.6649670022431948 1103 60-61 0.8806570266087661 1103 62-63 0.8115992984320641 1103 64-65 0.7426380855051775 1103 66-67 0.7178807804625755 1103 68-69 0.6961581667907026 1103 70-71 0.5371741999528616 1103 72-73 0.5704625784972102 1103 74-75 0.4762511803580729 1103 76-77 0.49553820572985074 1103 78-79 0.7631344612451656 1103 80-81 0.946561571558707 1103 82-83 0.9556247907633804 1103 84-85 0.9523830353213434 1103 86-87 1.015742178024066 1103 88-89 0.8103000124971373 1103 90-91 0.9438472450534015 1103 92-93 1.1326702635800778 1103 94-95 1.1834385772498557 1103 96-97 1.3454943265832817 1103 98-99 1.3076160255171096 1103 100-101 1.4187479643840923 1104 1 0.20821609286653242 1104 2 0.24762494645949573 1104 3 0.1990548579322109 1104 4 0.21424271212195123 1104 5 0.13516944829974875 1104 6 0.09000973010854096 1104 7 0.11470119608561191 1104 8 0.213552267124129 1104 9 0.23964086272404472 1104 10-11 0.20706208634424428 1104 12-13 0.2918076235732201 1104 14-15 0.37281842957504807 1104 16-17 0.4070687117859748 1104 18-19 0.33129749751835647 1104 20-21 0.3261939047573179 1104 22-23 0.38737724221585523 1104 24-25 0.37839000391843314 1104 26-27 0.380282705272208 1104 28-29 0.4459330534970505 1104 30-31 0.4310942615716655 1104 32-33 0.473962947757677 1104 34-35 0.45331866977501534 1104 36-37 0.5037632483291858 1104 38-39 0.474339297747818 1104 40-41 0.4074601895444019 1104 42-43 0.4848473735188108 1104 44-45 0.5675371696225184 1104 46-47 0.5467316116638514 1104 48-49 0.5595042713771434 1104 50-51 0.5303670941522398 1104 52-53 0.5277588293748536 1104 54-55 0.6072512235344263 1104 56-57 0.6230303652802505 1104 58-59 0.6847144489209853 1104 60-61 0.8175849827258688 1104 62-63 0.7135577397094508 1104 64-65 0.701992173905893 1104 66-67 0.6221805495658792 1104 68-69 0.834774376150154 1104 70-71 0.731013016263006 1104 72-73 0.6809748615397169 1104 74-75 0.8538356897115342 1104 76-77 0.8479725137417518 1104 78-79 1.074387813584405 1104 80-81 0.8933331537376326 1104 82-83 0.9126137976840205 1104 84-85 1.0829795080210722 1104 86-87 1.3056493230338084 1104 88-89 1.2943533740472013 1104 90-91 1.3781972869492591 1104 92-93 1.7386229069537755 1104 94-95 1.7353520486681155 1104 96-97 1.6156674387434542 1104 98-99 1.71105613590397 1104 100-101 1.784628573264584 1105 1 0.15845000678130106 1105 2 0.18068978985312611 1105 3 0.1508035510130128 1105 4 0.11174727025210274 1105 5 0.09182947876104919 1105 6 0.13875085967733014 1105 7 0.1633264254343203 1105 8 0.11219432560433518 1105 9 0.19002025501460906 1105 10-11 0.1478272144957984 1105 12-13 0.26370229219455865 1105 14-15 0.40606102509806874 1105 16-17 0.24112466463629545 1105 18-19 0.28122493139413507 1105 20-21 0.269297647621201 1105 22-23 0.1994145132093479 1105 24-25 0.21283886375919536 1105 26-27 0.1555252225494641 1105 28-29 0.19648782746907045 1105 30-31 0.3066143834756261 1105 32-33 0.2567196801182732 1105 34-35 0.32378312968052114 1105 36-37 0.37304688952182374 1105 38-39 0.25399095308090835 1105 40-41 0.25236508476064046 1105 42-43 0.14677031235401472 1105 44-45 0.03940975951415027 1105 46-47 0.09304219299732353 1105 48-49 0.19273367210586656 1105 50-51 0.1372857289462388 1105 52-53 0.15513626541655157 1105 54-55 0.09599217269744287 1105 56-57 0.10630145337773911 1105 58-59 0.09881859318770836 1105 60-61 0.13691038470821582 1105 62-63 0.05968708666258493 1105 64-65 0.0018949016306777366 1105 66-67 -0.008349372462117088 1105 68-69 -0.025778517196574313 1105 70-71 0.2339198042714301 1105 72-73 0.21351282567945518 1105 74-75 0.3370693331225354 1105 76-77 0.07433146479965913 1105 78-79 0.34698985114174263 1105 80-81 0.2891714233190257 1105 82-83 0.34452514544834756 1105 84-85 0.47951658775297545 1105 86-87 0.5429419127723065 1105 88-89 0.6828544779312438 1105 90-91 0.6309725203413628 1105 92-93 0.8143251023346956 1105 94-95 0.9560721131359102 1105 96-97 1.0838083037660589 1105 98-99 1.1703443218536584 1105 100-101 1.0859696699198054 1106 1 -0.012268927528236162 1106 2 0.12995182535677685 1106 3 0.05468803342098738 1106 4 0.043632275627039974 1106 5 -0.027299490703491358 1106 6 -0.05354482003445327 1106 7 0.023114623813121682 1106 8 0.06400001432277946 1106 9 -0.24684818973973677 1106 10-11 0.02771296074060814 1106 12-13 0.06063058761279194 1106 14-15 -0.006618018453451668 1106 16-17 0.08204937192006412 1106 18-19 0.08734618709593178 1106 20-21 0.13379036966425417 1106 22-23 0.1718127525538904 1106 24-25 0.20541600138667349 1106 26-27 0.1377748521145321 1106 28-29 0.17394831440942227 1106 30-31 0.04754018027390572 1106 32-33 0.038569821028815454 1106 34-35 -0.0386968958867584 1106 36-37 0.06711303031615756 1106 38-39 -0.09956594863035662 1106 40-41 -0.08114511309519656 1106 42-43 -0.036057849417098 1106 44-45 -0.055351854710544046 1106 46-47 -0.7588001760430316 1106 48-49 -0.5169473906792135 1106 50-51 -0.12606663457390965 1106 52-53 -0.09740702462674022 1106 54-55 -0.03365024863960997 1106 56-57 -0.14098166060000494 1106 58-59 -0.27049559374393084 1106 60-61 -0.21021402332223005 1106 62-63 -0.3371736588558818 1106 64-65 -0.33989270114685866 1106 66-67 -0.3425986243288186 1106 68-69 -0.4951813179752129 1106 70-71 -0.45331290150154047 1106 72-73 0.07986135410852313 1106 74-75 0.0627771543840474 1106 76-77 -0.01979496246435275 1106 78-79 0.18382742631823845 1106 80-81 -0.5043859141980427 1106 82-83 0.08331331477343085 1106 84-85 0.24114679200063094 1106 86-87 0.6576354686571406 1106 88-89 0.6590645653829448 1106 90-91 0.5150590713742211 1106 92-93 0.46985620434231024 1106 94-95 0.516021559662537 1106 96-97 0.6447786253907424 1106 98-99 0.8144740176612046 1106 100-101 0.813427977831136 1107 1 0.04268395287314419 1107 2 0.10869237030517809 1107 3 0.11975083946740028 1107 4 0.033583910753435475 1107 5 0.011808972747694213 1107 6 0.06905601711169851 1107 7 0.025384438749192384 1107 8 0.05764589828725519 1107 9 0.09752828902430366 1107 10-11 0.10624075447486803 1107 12-13 0.11187154430533752 1107 14-15 0.11477821599567051 1107 16-17 0.19951611102691658 1107 18-19 0.20826394471720988 1107 20-21 0.20598809924676686 1107 22-23 0.2648708466404486 1107 24-25 0.19722937282809738 1107 26-27 0.17320513479194943 1107 28-29 0.20549479530248504 1107 30-31 0.24683007225633702 1107 32-33 0.08500050139083015 1107 34-35 0.23415008391970815 1107 36-37 0.19209871969636794 1107 38-39 0.304880988595734 1107 40-41 0.2201146733602286 1107 42-43 0.18844208229362636 1107 44-45 0.21063848034876287 1107 46-47 0.20193926623445435 1107 48-49 0.30755113507587595 1107 50-51 0.3267542145429445 1107 52-53 0.3290643581507098 1107 54-55 0.24233417136353097 1107 56-57 0.25486017541736317 1107 58-59 0.33120350363255824 1107 60-61 0.32379935735833243 1107 62-63 0.39158918053175995 1107 64-65 0.47272760848474604 1107 66-67 0.4308094321986289 1107 68-69 0.5618547869653163 1107 70-71 0.6251901875838222 1107 72-73 0.6661804015936674 1107 74-75 0.6285864726562487 1107 76-77 0.6188473764834299 1107 78-79 0.7528060698522516 1107 80-81 0.6820761390595571 1107 82-83 0.7377207848165348 1107 84-85 0.9040329778840324 1107 86-87 1.0324939190530174 1107 88-89 1.0240513494000396 1107 90-91 1.1599965264338508 1107 92-93 1.3109118159129238 1107 94-95 1.245324486862156 1107 96-97 1.3311743476703164 1107 98-99 1.3729007131432667 1107 100-101 1.5503551513381097 1108 1 0.12961418935487856 1108 2 0.15337354252849167 1108 3 0.1086562873892376 1108 4 0.12772318471795074 1108 5 0.07556847466447891 1108 6 0.23640828241865108 1108 7 0.08177263247112876 1108 8 0.14328392485668928 1108 9 0.18469041054886048 1108 10-11 0.1418949592976091 1108 12-13 0.1919256814078878 1108 14-15 0.32570070449026645 1108 16-17 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2316 66-67 -0.8016404667307917 2316 68-69 -0.7216867257507182 2316 70-71 -0.7120942728633004 2316 72-73 -0.610227174725992 2316 74-75 -0.6890513627579757 2316 76-77 -0.6796129981264372 2316 78-79 -0.6727605596507722 2316 80-81 -0.8098375365306971 2316 82-83 -0.9232866768747918 2316 84-85 -1.0111666501841938 2316 86-87 -1.2582219456327515 2316 88-89 -1.2927989248245986 2316 90-91 -1.3522259747693504 2316 92-93 -1.5651137292610642 2316 94-95 -1.2782198902059214 2316 96-97 -1.3038469853329389 2316 98-99 -1.5744431744714227 2316 100-101 -1.8281675247370348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 62.0 5 124.0 6 256.0 7 660.0 8 1522.0 9 2717.0 10 4629.0 11 7257.0 12 10222.0 13 14301.0 14 18395.0 15 24714.0 16 31946.0 17 40347.0 18 36554.0 19 28222.0 20 27299.0 21 29346.0 22 31386.0 23 35207.0 24 40786.0 25 47749.0 26 56189.0 27 67435.0 28 80634.0 29 95822.0 30 113511.0 31 135490.0 32 162909.0 33 200509.0 34 254031.0 35 331842.0 36 431912.0 37 473184.0 38 215394.0 39 7345.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.499625806476914 19.28426746634056 15.67086525961187 22.545241467570655 2 16.159407258209548 20.866126284606302 38.73895230403521 24.23551415314894 3 19.057749995016227 24.572743633761675 29.978943969651606 26.390562401570495 4 13.654490901919031 17.377614070382634 35.79100313046275 33.17689189723559 5 14.125198175461588 37.34788020507911 33.21301397698957 15.313907642469724 6 32.27634871505273 35.896495705295315 16.94917303690835 14.877982542743611 7 29.04206009874079 32.95028548029351 19.759922691067217 18.247731729898483 8 27.24633333235292 34.08312148215107 19.366515127301888 19.304030058194122 9 27.217607194410594 16.402297960313483 19.603448574494 36.776646270781924 10-11 25.485787097813645 26.498832816818418 26.490384913908187 21.52499517145975 12-13 26.47211652811779 23.85841380083407 27.125252170718884 22.544217500329257 14-15 23.175016037943337 25.112249263136977 25.92604212317206 25.786692575747622 16-17 22.42364288606508 28.36114605198614 26.43185418233287 22.783356879615912 18-19 22.433626771322622 26.431576398291334 29.43857230767622 21.696224522709826 20-21 23.171388505165638 25.522781396051087 29.55200623804916 21.753823860734116 22-23 23.06901691574091 25.248968032285696 29.423604649908775 22.25841040206462 24-25 22.835747994155724 25.62965592084181 29.38620895554583 22.14838712945664 26-27 22.190657664313132 26.12999619427841 29.413342052667986 22.266004088740473 28-29 22.33117903427793 26.19125351969066 29.447653670282435 22.029913775748973 30-31 22.3682045411206 25.916525906950376 29.207533554790565 22.507735997138454 32-33 22.3272305514269 25.895187972480286 29.073330771171495 22.70425070492131 34-35 23.06094920714193 26.064252945844572 28.776567510926395 22.098230336087102 36-37 23.11865371892398 25.59115371238648 28.936884330651214 22.35330823803833 38-39 22.478098575081717 25.988242001614818 28.80364809409275 22.73001132921072 40-41 22.773793165879958 25.73789669029655 28.82259252886495 22.665717614958545 42-43 22.803104429380703 25.780437571857252 28.559528034704563 22.856929964057485 44-45 22.546459280016588 26.133008987332236 28.578186947034435 22.742344785616737 46-47 22.871512462209115 26.47250869382349 27.846461243733927 22.80951760023347 48-49 22.812556884452622 26.228859408921313 27.99231420577784 22.96626950084822 50-51 23.14037135708478 26.555681993469094 27.515006967520634 22.788939681925495 52-53 23.31225769469965 26.50167601818472 27.31507671251411 22.87098957460152 54-55 22.758291400956963 26.592517958996524 27.565576768030976 23.083613872015533 56-57 22.75925502895327 26.670699437340257 27.64660379596795 22.923441737738532 58-59 22.901244939145535 26.237404658503056 27.512367345631272 23.34898305672014 60-61 22.768274840184304 26.52071239514878 27.721442084805183 22.989570679861735 62-63 22.47965587099117 26.792282753692277 27.646199348850605 23.081862026465956 64-65 22.286550468347254 26.474939221737536 27.584636876438562 23.653873433476647 66-67 22.41080322849695 26.538458018819828 27.528914108071508 23.52182464461172 68-69 22.53831156202657 26.392886968430652 27.605995185417008 23.46280628412577 70-71 22.513781370019164 26.613273253488316 27.239742242301123 23.633203134191394 72-73 22.65210685409582 26.69587648996488 27.052845498564594 23.599171157374705 74-75 22.443249561698057 26.893262546908975 26.980130257437324 23.683357633955648 76-77 22.53951625085585 26.523364467646402 27.19091134404626 23.746207937451487 78-79 22.71224705018558 26.620362954502212 27.317223307178867 23.350166688133335 80-81 22.673295775863362 26.974055402607405 26.999585503609847 23.353063317919386 82-83 22.674045969605 26.84164232317273 26.88401152664642 23.600300180575847 84-85 22.63273033207669 26.552538116029094 27.01555888365124 23.799172668242974 86-87 22.72505842569531 26.675977083040888 26.782328925593095 23.816635565670712 88-89 22.55444581764766 26.714025005817987 26.757118183407506 23.974410993126842 90-91 22.455467546024675 26.61753401990683 26.959535028993674 23.96746340507482 92-93 22.501276485021158 26.222352903488577 26.964348639603656 24.3120219718866 94-95 22.33351148768692 26.3518237939032 26.952612576389807 24.362052142020076 96-97 22.430958209993097 26.215635212910758 26.903261968859166 24.450144608236972 98-99 22.36348197078521 26.2797083621891 26.56605894620369 24.790750720821993 100-101 22.418602005968584 26.172067215129697 26.858743668736217 24.5505871101655 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1234.0 1 849.5 2 555.5 3 699.0 4 645.5 5 620.5 6 1113.5 7 2472.5 8 3841.0 9 4191.0 10 3597.5 11 3047.0 12 3141.0 13 3404.5 14 3800.5 15 4230.5 16 4685.5 17 5092.5 18 5405.5 19 5955.5 20 6466.5 21 7300.0 22 8442.0 23 9637.0 24 11169.0 25 13361.0 26 16345.5 27 20367.0 28 24009.0 29 27457.0 30 32002.5 31 37011.0 32 43303.0 33 46988.0 34 48614.5 35 50961.0 36 55083.0 37 61295.0 38 65623.5 39 69050.0 40 74168.0 41 83006.5 42 91997.5 43 99087.5 44 103605.0 45 107577.5 46 109650.0 47 113440.5 48 119114.0 49 120734.0 50 122112.5 51 119522.0 52 115705.0 53 115277.0 54 116032.5 55 109155.0 56 96198.0 57 86582.0 58 79629.0 59 70782.5 60 58581.0 61 48389.5 62 41034.0 63 32456.5 64 25271.5 65 20356.5 66 15540.0 67 12325.5 68 9514.0 69 6867.0 70 5387.5 71 4404.5 72 3683.0 73 3002.5 74 2351.5 75 1910.5 76 1294.5 77 721.0 78 423.0 79 260.5 80 144.5 81 77.0 82 44.0 83 27.0 84 15.5 85 9.0 86 7.0 87 4.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.555680830711542E-4 2 0.05232144123511282 3 0.0 4 0.0 5 0.04588338756658238 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006029547725095762 26-27 0.0021078906681229084 28-29 9.804142642432133E-5 30-31 0.0015523225850517545 32-33 4.902071321216067E-4 34-35 0.011993734499241977 36-37 0.02153643333787592 38-39 0.00462428727968049 40-41 0.0011274764038796954 42-43 0.00147062139636482 44-45 0.007696251974309225 46-47 0.0 48-49 0.0 50-51 6.209290340207017E-4 52-53 0.0 54-55 0.0014379409208900463 56-57 0.0 58-59 0.02173251619072456 60-61 0.0 62-63 0.008643985763077665 64-65 0.014673533488173426 66-67 0.005849805109984506 68-69 0.015555906325992318 70-71 9.804142642432133E-5 72-73 4.9020713212160664E-5 74-75 0.02227174403605833 76-77 0.05127566601992006 78-79 0.030229439814165742 80-81 0.0260463389533947 82-83 0.03612826563736241 84-85 0.014934977291971617 86-87 0.009003470993300176 88-89 0.08505093742309876 90-91 0.02384040685884747 92-93 0.02447767613060556 94-95 0.025686853723172188 96-97 0.037566206558252456 98-99 0.0528443288427092 100-101 0.01344801565786941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.352541488062876 #Duplication Level Percentage of deduplicated Percentage of total 1 67.09468799991633 27.074411811452574 2 15.533033015437953 12.535947183818209 3 6.44844433718807 7.80633352949537 4 3.4225506907523733 5.524344749743335 5 1.9907053757067306 4.016501063185783 6 1.2274347872295557 2.9718067905343366 7 0.8490644715716388 2.3983336520594314 8 0.5928183810265771 1.9137382652208976 9 0.44785529528737966 1.6264889445359376 >10 2.135074495773532 15.250463329952671 >50 0.1454946358169111 4.048036434790165 >100 0.09775981986861076 7.82770492620239 >500 0.010132187113385158 2.8226351588691303 >1k 0.004701334820525378 3.0705348513855224 >5k 8.105749690560997E-5 0.2735433604488642 >10k+ 1.6211499381121994E-4 0.8391759483051607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14130 0.4617751184585535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11526 0.3766751603222426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8363 0.2733068163955331 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3653 0.11938177690934862 No Hit ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT 3376 0.11032928520283626 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3083 0.10075390588872755 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6.536095094954755E-5 6 0.0 0.0 0.0 0.0 6.536095094954755E-5 7 0.0 0.0 0.0 0.0 6.536095094954755E-5 8 0.0 0.0 0.0 0.0 6.536095094954755E-5 9 0.0 0.0 0.0 0.0 6.536095094954755E-5 10-11 0.0 0.0 0.0 1.6340237737386888E-5 6.536095094954755E-5 12-13 0.0 0.0 0.0 3.2680475474773776E-5 9.804142642432133E-5 14-15 0.0 0.0 0.0 3.2680475474773776E-5 1.47062139636482E-4 16-17 0.0 0.0 0.0 3.2680475474773776E-5 1.9608285284864266E-4 18-19 0.0 0.0 0.0 8.170118868693444E-5 1.9608285284864266E-4 20-21 0.0 0.0 0.0 1.47062139636482E-4 1.9608285284864266E-4 22-23 0.0 0.0 0.0 5.065473698589936E-4 1.9608285284864266E-4 24-25 0.0 0.0 0.0 0.0011928373548292428 1.9608285284864266E-4 26-27 0.0 0.0 0.0 0.00267979898893145 2.1242309058602956E-4 28-29 0.0 0.0 0.0 0.0066177962836416904 2.2876332832341643E-4 30-31 0.0 0.0 0.0 0.018872974586681857 2.2876332832341643E-4 32-33 0.0 0.0 0.0 0.042468277879468525 2.614438037981902E-4 34-35 0.0 0.0 0.0 0.07742004639973908 3.2680475474773776E-4 36-37 0.0 0.0 0.0 0.13421871277489592 3.2680475474773776E-4 38-39 3.2680475474773776E-5 0.0 0.0 0.22029908517545005 3.2680475474773776E-4 40-41 3.2680475474773776E-5 0.0 0.0 0.31616725998069894 3.2680475474773776E-4 42-43 3.2680475474773776E-5 0.0 0.0 0.4113328045632402 3.2680475474773776E-4 44-45 3.2680475474773776E-5 0.0 0.0 0.525273282306039 3.4314499248512466E-4 46-47 3.2680475474773776E-5 0.0 0.0 0.6597370986469957 3.5948523022251156E-4 48-49 3.2680475474773776E-5 0.0 0.0 0.8055246997399614 3.921657056972853E-4 50-51 3.2680475474773776E-5 0.0 0.0 0.9646295945888976 4.248461811720591E-4 52-53 4.9020713212160664E-5 0.0 0.0 1.122688714222641 4.5752665664683287E-4 54-55 6.536095094954755E-5 0.0 0.0 1.297120752069246 4.5752665664683287E-4 56-57 6.536095094954755E-5 0.0 0.0 1.4931872646801514 4.5752665664683287E-4 58-59 6.536095094954755E-5 0.0 0.0 1.7084535566324863 4.5752665664683287E-4 60-61 6.536095094954755E-5 0.0 0.0 1.9447660747905753 4.5752665664683287E-4 62-63 6.536095094954755E-5 0.0 0.0 2.1781700306314096 4.902071321216066E-4 64-65 6.536095094954755E-5 0.0 0.0 2.419858487005099 5.228876075963804E-4 66-67 6.536095094954755E-5 0.0 0.0 2.6993909339785764 5.228876075963804E-4 68-69 6.536095094954755E-5 0.0 0.0 3.010672462875797 5.555680830711542E-4 70-71 6.536095094954755E-5 0.0 0.0 3.3434740848731557 5.719083208085411E-4 72-73 6.536095094954755E-5 0.0 0.0 3.684445825739208 6.04588796283315E-4 74-75 6.536095094954755E-5 0.0 0.0 4.04017280128212 6.209290340207018E-4 76-77 6.536095094954755E-5 0.0 0.0 4.420851319850023 6.209290340207018E-4 78-79 6.536095094954755E-5 0.0 0.0 4.8402562018555315 6.209290340207018E-4 80-81 6.536095094954755E-5 0.0 0.0 5.287717272056135 6.209290340207018E-4 82-83 6.536095094954755E-5 0.0 0.0 5.747074035329555 7.189704604450231E-4 84-85 6.536095094954755E-5 0.0 0.0 6.2187186573814905 7.189704604450231E-4 86-87 6.536095094954755E-5 0.0 0.0 6.701343919192949 7.516509359197969E-4 88-89 6.536095094954755E-5 0.0 0.0 7.2204732721097304 7.843314113945706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7115 0.0 56.176025 1 GTATCAA 13145 0.0 44.036884 1 ATCAACG 17765 0.0 32.330048 3 TATCAAC 17780 0.0 32.28978 2 TCAACGC 18070 0.0 31.836933 4 CAACGCA 18330 0.0 31.385344 5 AACGCAG 18805 0.0 30.552828 6 ACGCAGA 21635 0.0 26.468529 7 CGCAGAG 21960 0.0 26.076805 8 GTACATG 15095 0.0 24.967207 1 TACATGG 15160 0.0 24.67206 2 TGGTATC 3030 0.0 24.468754 2 GCAGAGT 23410 0.0 24.05596 9 GGACCGA 1560 0.0 23.43719 6 GTGGTAT 3225 0.0 23.431349 1 ACATGGG 15690 0.0 22.88991 3 AGGACCG 1840 0.0 22.720093 5 GTGTAAG 1130 0.0 22.711466 1 AGTACTT 13730 0.0 22.410103 12-13 GAGTACT 13415 0.0 22.369991 12-13 >>END_MODULE