FastQCFastQC Report
Thu 26 May 2016
SRR938626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035213
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148520.4893231545858561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112220.36972693514425514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79810.2629469496868918No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG34410.11336930884257547No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG33040.10885562232370513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.055.0549431
GTATCAA136400.043.845791
TATCAAC178900.033.25782
ATCAACG182400.032.5935823
TCAACGC184650.032.2478944
CAACGCA187150.031.58655
AACGCAG194450.030.444546
GTGGTAT34000.026.8890191
TGGTATC32750.026.844792
ACGCAGA223150.026.375597
CGCAGAG225700.025.9938328
GTACATG155450.024.2597921
GCAGAGT238250.023.9466639
TACATGG156350.023.677682
GAGTACT133150.023.06394812-13
AGTACTT137250.021.99426712-13
ACATGGG163000.021.9536613
GTACTTT141150.021.50594114-15
AGAGTAC222000.020.78823310-11
GTGTAAG10550.020.7614651
Produced by FastQC (version 0.11.5)