##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938626_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3035213 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.147117846424617 31.0 31.0 34.0 28.0 34.0 2 31.241895379335816 31.0 31.0 34.0 28.0 34.0 3 31.299439281526535 31.0 31.0 34.0 28.0 34.0 4 34.93901185847583 37.0 35.0 37.0 32.0 37.0 5 34.95616683244306 37.0 35.0 37.0 32.0 37.0 6 35.00394140378287 37.0 35.0 37.0 32.0 37.0 7 34.931599199133636 37.0 35.0 37.0 32.0 37.0 8 34.893314571333214 37.0 35.0 37.0 32.0 37.0 9 36.398256399139036 39.0 37.0 39.0 32.0 39.0 10-11 36.2501549314661 39.0 35.0 39.0 32.0 39.0 12-13 36.10268900403365 39.0 35.0 39.0 31.0 39.0 14-15 37.07480661159529 39.0 36.0 41.0 31.0 41.0 16-17 36.94274965216609 39.0 36.0 41.0 31.0 41.0 18-19 36.96060787167161 39.0 36.0 41.0 31.0 41.0 20-21 37.056961241270386 39.0 36.0 41.0 31.0 41.0 22-23 36.983133144197794 39.0 36.0 41.0 31.0 41.0 24-25 36.868905411251205 39.0 36.0 41.0 30.0 41.0 26-27 36.6649121494933 39.0 36.0 41.0 30.0 41.0 28-29 36.48095800854833 39.0 35.5 41.0 30.0 41.0 30-31 36.229837576473216 39.0 35.0 40.0 29.5 41.0 32-33 35.92759519677861 38.0 35.0 40.0 29.0 41.0 34-35 35.65833007436381 38.0 35.0 40.0 27.0 41.0 36-37 35.355672237829765 38.0 34.0 40.0 26.5 41.0 38-39 35.084231979765505 38.0 34.0 40.0 25.5 41.0 40-41 34.98264191013942 38.0 34.0 40.0 25.0 41.0 42-43 34.76067396258516 38.0 34.0 40.0 24.5 41.0 44-45 34.40388747017096 38.0 33.0 40.0 23.5 41.0 46-47 33.95218193912585 38.0 33.0 40.0 22.5 41.0 48-49 33.525689300882675 38.0 33.0 40.0 19.5 41.0 50-51 32.756062095147854 37.0 31.5 39.5 18.0 40.5 52-53 32.79877491299622 37.0 31.5 39.5 17.5 40.0 54-55 32.910359668333 37.0 32.0 40.0 15.0 41.0 56-57 32.60077579398876 37.0 31.5 40.0 12.5 41.0 58-59 32.27022897569297 36.0 31.0 39.5 8.5 41.0 60-61 31.818784217120843 36.0 31.0 39.0 7.5 41.0 62-63 31.291657949540937 35.0 30.0 39.0 4.5 40.0 64-65 30.79658000937661 35.0 29.0 38.5 2.0 40.0 66-67 30.257732818092173 35.0 29.0 38.0 2.0 40.0 68-69 29.697950358014413 34.0 28.0 37.5 2.0 40.0 70-71 29.081370895551647 34.0 27.5 37.0 2.0 39.0 72-73 28.538488237893024 34.0 26.0 36.0 2.0 39.0 74-75 27.934597835473163 33.5 26.0 36.0 2.0 38.5 76-77 27.21474275446237 33.0 24.5 35.0 2.0 37.5 78-79 26.43555592309337 32.0 22.5 35.0 2.0 37.0 80-81 25.79469315662525 32.0 20.5 35.0 2.0 36.0 82-83 25.144650144816854 31.0 19.0 34.5 2.0 36.0 84-85 24.49170914858364 31.0 16.5 34.0 2.0 35.0 86-87 23.825396438404816 31.0 8.5 34.0 2.0 35.0 88-89 23.043903179117905 30.0 2.0 34.0 2.0 35.0 90-91 22.216935187085717 29.0 2.0 34.0 2.0 35.0 92-93 21.366896985483393 29.0 2.0 34.0 2.0 35.0 94-95 20.40447391995224 28.0 2.0 34.0 2.0 35.0 96-97 19.169583485574158 26.0 2.0 33.0 2.0 35.0 98-99 17.69858589825492 23.5 2.0 33.0 2.0 34.0 100-101 14.311375017173424 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6917912917756794 1101 2 0.7062400365708363 1101 3 0.7524254046753107 1101 4 0.624367863391079 1101 5 0.6735176358779711 1101 6 0.645439999676455 1101 7 0.5456856220047115 1101 8 0.6139341102765741 1101 9 0.6635993885030871 1101 10-11 0.7402731163845928 1101 12-13 0.8348811332884409 1101 14-15 0.9679254613554633 1101 16-17 0.9305382833085147 1101 18-19 0.9574245294080939 1101 20-21 1.0633071398360912 1101 22-23 1.124366998043385 1101 24-25 1.0978269819566222 1101 26-27 1.1002092444689993 1101 28-29 1.0246275067400603 1101 30-31 1.0909920086850988 1101 32-33 1.148720529576856 1101 34-35 1.1892212015203327 1101 36-37 1.1281677052021664 1101 38-39 1.217260518732914 1101 40-41 1.1961565100765057 1101 42-43 1.211501387831369 1101 44-45 1.3686145654240534 1101 46-47 1.5103030668327904 1101 48-49 1.5408931504373342 1101 50-51 1.452442891589854 1101 52-53 1.4983948166180951 1101 54-55 1.4483116812187333 1101 56-57 1.370698303792544 1101 58-59 1.4360357676277644 1101 60-61 1.351822691099997 1101 62-63 1.403839758185459 1101 64-65 1.3573373456292757 1101 66-67 1.277483859014886 1101 68-69 1.337550507966018 1101 70-71 1.4943790867823914 1101 72-73 1.5839428014174821 1101 74-75 1.5985025817844587 1101 76-77 1.7084019049307422 1101 78-79 1.4127104051732609 1101 80-81 1.5129013566754956 1101 82-83 1.4406055938823172 1101 84-85 1.6219241387349683 1101 86-87 1.6053202191472522 1101 88-89 1.8547753534530855 1101 90-91 1.7790912192174346 1101 92-93 1.8779437926499618 1101 94-95 1.6329966556740168 1101 96-97 1.7282543135903659 1101 98-99 1.661736188911778 1101 100-101 1.5924916241679465 1102 1 0.5652756641227548 1102 2 0.6002841236954453 1102 3 0.5603654907653635 1102 4 0.4832609636571732 1102 5 0.549962791141553 1102 6 0.5007788785553089 1102 7 0.43604830440189346 1102 8 0.5328708173365229 1102 9 0.6152846745845437 1102 10-11 0.6345506452625713 1102 12-13 0.7322607668873076 1102 14-15 0.7845575610481035 1102 16-17 0.8198554105686497 1102 18-19 0.859251558305985 1102 20-21 0.7093879512758647 1102 22-23 0.8038523504059683 1102 24-25 0.6802319837305291 1102 26-27 0.7153946839370846 1102 28-29 0.6967518091011087 1102 30-31 0.6870122886468053 1102 32-33 0.6597830923437797 1102 34-35 0.7786819253066497 1102 36-37 0.7766286799619238 1102 38-39 0.886561149861393 1102 40-41 0.779215023084582 1102 42-43 0.9500192897033983 1102 44-45 0.8870637531033125 1102 46-47 0.8325395280594066 1102 48-49 0.730145982246043 1102 50-51 0.9044199955724181 1102 52-53 0.8689468714899178 1102 54-55 0.7793961818550841 1102 56-57 0.9360215273070835 1102 58-59 1.0520473332685114 1102 60-61 1.1289052963024204 1102 62-63 0.8992178628193948 1102 64-65 0.8824269614991458 1102 66-67 0.9255583195822048 1102 68-69 0.8257570180187166 1102 70-71 0.7669163328382922 1102 72-73 0.8478151168844512 1102 74-75 0.8408694763128963 1102 76-77 0.7524618208514937 1102 78-79 0.9575520210702706 1102 80-81 1.1859007357141458 1102 82-83 1.2093924395054962 1102 84-85 1.1371878990109607 1102 86-87 1.1114373984871975 1102 88-89 1.306347029119987 1102 90-91 1.4285831707867231 1102 92-93 1.3764112459682103 1102 94-95 1.3852175570494936 1102 96-97 1.433493412614535 1102 98-99 1.5028069695824726 1102 100-101 1.3503696078394665 1103 1 0.57679052205593 1103 2 0.5653689921581879 1103 3 0.5157460428212062 1103 4 0.5710527152209437 1103 5 0.583064516938137 1103 6 0.6156567896264704 1103 7 0.547655640657446 1103 8 0.42430348663754813 1103 9 0.49839787514005707 1103 10-11 0.5479708144159119 1103 12-13 0.5760986065249583 1103 14-15 0.7207557532275786 1103 16-17 0.7132609865496278 1103 18-19 0.772558232963803 1103 20-21 0.7363778015741147 1103 22-23 0.7291221325502377 1103 24-25 0.8329468830516049 1103 26-27 0.8389432880442982 1103 28-29 0.7506577561768708 1103 30-31 0.9157493455304575 1103 32-33 1.0365051564971708 1103 34-35 0.9020885717877647 1103 36-37 1.0952512291342273 1103 38-39 0.9525249025121312 1103 40-41 0.9421077292552056 1103 42-43 0.8980690640211932 1103 44-45 0.9832514121343365 1103 46-47 1.0446621722878149 1103 48-49 0.9786212356126569 1103 50-51 0.8978305123232388 1103 52-53 0.9797287769586731 1103 54-55 0.9919065691076128 1103 56-57 1.0880456139180623 1103 58-59 1.0905364673341538 1103 60-61 1.1375333841493074 1103 62-63 1.2162462976658581 1103 64-65 1.1263615399780846 1103 66-67 1.1322652714243802 1103 68-69 1.2344705668627043 1103 70-71 1.2245843181176603 1103 72-73 1.2218838444249798 1103 74-75 1.1835168768414164 1103 76-77 1.171832037389681 1103 78-79 1.146793351540822 1103 80-81 1.2450895472334444 1103 82-83 1.282789544572399 1103 84-85 1.057854888617804 1103 86-87 1.2999753853096614 1103 88-89 1.2337517250710945 1103 90-91 1.4227437536829939 1103 92-93 1.5308362602982335 1103 94-95 1.43642469810883 1103 96-97 1.4212710937250748 1103 98-99 1.4957882122004094 1103 100-101 1.0923715576534168 1104 1 0.5410936113971268 1104 2 0.41896605034481027 1104 3 0.465738009574153 1104 4 0.5352987944217702 1104 5 0.4824347464231096 1104 6 0.48664441932735514 1104 7 0.5095790636454183 1104 8 0.38589420024901955 1104 9 0.513160121447747 1104 10-11 0.536117099281384 1104 12-13 0.49164411132045416 1104 14-15 0.6144233913199884 1104 16-17 0.6502973989037457 1104 18-19 0.7141234024270133 1104 20-21 0.7774739443311134 1104 22-23 0.7751400193127722 1104 24-25 0.7756926468939724 1104 26-27 0.6954840299017064 1104 28-29 0.6414761266017237 1104 30-31 0.6648999395062631 1104 32-33 0.7503752053749153 1104 34-35 0.6997400356752195 1104 36-37 0.7408661691259795 1104 38-39 0.77023880940353 1104 40-41 0.7142067637558114 1104 42-43 0.7214055115052815 1104 44-45 0.7135722665563549 1104 46-47 0.7737118470483466 1104 48-49 0.8786538981416925 1104 50-51 0.664718639724903 1104 52-53 0.7084745854176688 1104 54-55 0.6887520493189001 1104 56-57 0.801319309687166 1104 58-59 0.8077503412137474 1104 60-61 0.9838719585848352 1104 62-63 1.092301971109972 1104 64-65 1.0054066967413249 1104 66-67 0.8904747879716268 1104 68-69 0.9145136602193986 1104 70-71 0.884700902728035 1104 72-73 0.7152422194061359 1104 74-75 0.7316390123917031 1104 76-77 0.9352450812647319 1104 78-79 0.7724727693253293 1104 80-81 0.7767899456509504 1104 82-83 0.8115534700878619 1104 84-85 0.780576156126731 1104 86-87 0.9363814412737881 1104 88-89 1.0554098661304074 1104 90-91 0.9201434265394255 1104 92-93 1.059496474678891 1104 94-95 1.0150780171918825 1104 96-97 1.0870475684568497 1104 98-99 0.9434022026240569 1104 100-101 0.6572181699553941 1105 1 0.5293159472662339 1105 2 0.5271182241414145 1105 3 0.4806541988060715 1105 4 0.4869579234719481 1105 5 0.3977478094277842 1105 6 0.48857565458304464 1105 7 0.463020418175347 1105 8 0.3569027468327377 1105 9 0.5471551324590465 1105 10-11 0.5621923103321436 1105 12-13 0.5880740577647785 1105 14-15 0.6762119928327195 1105 16-17 0.6958978718785573 1105 18-19 0.6883536024986157 1105 20-21 0.7065804812860748 1105 22-23 0.7327632887479396 1105 24-25 0.6519425989558911 1105 26-27 0.7511339856915384 1105 28-29 0.692689533239438 1105 30-31 0.6637691899088978 1105 32-33 0.6684702841101355 1105 34-35 0.6925972611710378 1105 36-37 0.7476529163186356 1105 38-39 0.7572836359576485 1105 40-41 0.7686404312666895 1105 42-43 0.8717447524531252 1105 44-45 0.727552832939196 1105 46-47 0.7493680310397508 1105 48-49 0.6460925411436165 1105 50-51 0.633514857126201 1105 52-53 0.620155012903254 1105 54-55 0.7731904045852005 1105 56-57 0.8540978010312088 1105 58-59 0.8281038875285773 1105 60-61 0.9681983476269664 1105 62-63 1.031925909428704 1105 64-65 0.9837344514028885 1105 66-67 0.968063996193024 1105 68-69 0.921466985558407 1105 70-71 0.946864867472172 1105 72-73 1.0249143679042874 1105 74-75 1.1101964199344039 1105 76-77 1.286850037601976 1105 78-79 1.406083761469052 1105 80-81 1.2312677804735621 1105 82-83 1.5059820363438625 1105 84-85 1.3859267104623818 1105 86-87 1.3532852452330566 1105 88-89 1.2645469077753724 1105 90-91 1.4251243273640455 1105 92-93 1.404764230570244 1105 94-95 1.4811466915233567 1105 96-97 1.327619727431749 1105 98-99 1.2628736989071676 1105 100-101 0.8909486805800793 1106 1 0.31513263932842506 1106 2 0.41608837493827977 1106 3 0.19304513082460062 1106 4 0.4468159062820902 1106 5 0.41469264746987733 1106 6 0.02271649634718642 1106 7 0.17642056800611527 1106 8 0.25928319578265047 1106 9 0.20705497974692122 1106 10-11 0.15354977449734264 1106 12-13 0.1254408583864759 1106 14-15 0.41271551868615575 1106 16-17 0.13986885742260569 1106 18-19 -0.0933534427675653 1106 20-21 0.2858442808857333 1106 22-23 0.3995385184517204 1106 24-25 0.4195204359522222 1106 26-27 0.26772357948746617 1106 28-29 0.298391513242791 1106 30-31 0.3441545592061246 1106 32-33 0.4060213223322364 1106 34-35 0.36279008141310953 1106 36-37 0.4012615982824954 1106 38-39 0.449574639840705 1106 40-41 0.4987165627516532 1106 42-43 0.04076272960224259 1106 44-45 0.30512362119476677 1106 46-47 0.028387095055109057 1106 48-49 0.4735028418390499 1106 50-51 0.09856686357028366 1106 52-53 0.35747019267474656 1106 54-55 0.15369120840012584 1106 56-57 -0.24872848553876992 1106 58-59 -0.5918298750710171 1106 60-61 0.01410054407137551 1106 62-63 0.1653681377206162 1106 64-65 0.36506057441326334 1106 66-67 -0.02943864787925321 1106 68-69 -0.3893428103056529 1106 70-71 -0.33764694949701024 1106 72-73 0.08317331198724176 1106 74-75 0.07369852968374246 1106 76-77 0.2181173469533455 1106 78-79 0.41015214760190943 1106 80-81 0.4160705831054301 1106 82-83 0.37840290061651416 1106 84-85 0.3659992402463317 1106 86-87 0.5915679054579073 1106 88-89 0.34195567245124536 1106 90-91 0.48226552590845273 1106 92-93 0.6444997534270591 1106 94-95 0.4441035143315304 1106 96-97 0.4164501216358083 1106 98-99 0.2349784185761834 1106 100-101 0.12072661240708271 1107 1 0.34634616778519245 1107 2 0.41853665462245004 1107 3 0.38097871431061137 1107 4 0.38907595205285617 1107 5 0.3965273739210815 1107 6 0.43817627414379956 1107 7 0.4175606317722256 1107 8 0.3315812045586384 1107 9 0.40924017531589385 1107 10-11 0.40984529261330493 1107 12-13 0.443788122898475 1107 14-15 0.5264201391212993 1107 16-17 0.5214585929040823 1107 18-19 0.47792866034054526 1107 20-21 0.5651099436816622 1107 22-23 0.603382863610257 1107 24-25 0.6299814118084655 1107 26-27 0.4557046371051712 1107 28-29 0.5113515029411104 1107 30-31 0.6049538035320552 1107 32-33 0.6228970110127747 1107 34-35 0.5689563802401523 1107 36-37 0.5533799225638276 1107 38-39 0.7618745557320352 1107 40-41 0.7423436190131838 1107 42-43 0.7486615286085865 1107 44-45 0.9450239784936372 1107 46-47 0.7850446798320974 1107 48-49 0.8170933043063684 1107 50-51 0.6759507169864634 1107 52-53 0.6402177672152405 1107 54-55 0.7858353520890375 1107 56-57 0.7682470821947973 1107 58-59 0.8157785371006199 1107 60-61 0.7392738144644682 1107 62-63 0.6787254838636834 1107 64-65 0.6184243102077005 1107 66-67 0.7668891770964592 1107 68-69 0.7560708625702794 1107 70-71 0.6721335366380963 1107 72-73 0.5783613095487432 1107 74-75 0.7796614843759002 1107 76-77 0.7966833673741505 1107 78-79 0.7342352244798498 1107 80-81 0.6422110625248827 1107 82-83 0.6569260924429763 1107 84-85 0.72603857246256 1107 86-87 0.5297580122563623 1107 88-89 0.7212855985138518 1107 90-91 0.8263735175367053 1107 92-93 0.6799849349779201 1107 94-95 0.6907827701327491 1107 96-97 0.8244047078406815 1107 98-99 0.760840614008238 1107 100-101 0.528734377789517 1108 1 0.27585546135047423 1108 2 0.2684248843200905 1108 3 0.27895331267234624 1108 4 0.31308529589544065 1108 5 0.28539481975695935 1108 6 0.27899718640604476 1108 7 0.26575180926656117 1108 8 0.21887922518006775 1108 9 0.3286282619166059 1108 10-11 0.26112717035439204 1108 12-13 0.35233835765479427 1108 14-15 0.41169217798177726 1108 16-17 0.34109767924953616 1108 18-19 0.4052035147297559 1108 20-21 0.43153669739250944 1108 22-23 0.5197421297163984 1108 24-25 0.506186519982009 1108 26-27 0.487978408598714 1108 28-29 0.3189571046056727 1108 30-31 0.3205614240235306 1108 32-33 0.3530295497069531 1108 34-35 0.43232704769062735 1108 36-37 0.5300820024969894 1108 38-39 0.5443341598563194 1108 40-41 0.5794558070038676 1108 42-43 0.41881344340016113 1108 44-45 0.6526810177021929 1108 46-47 0.7200119609131264 1108 48-49 0.7473053198072392 1108 50-51 0.6385161741618077 1108 52-53 0.724499923415209 1108 54-55 0.6610675945339111 1108 56-57 0.6899444437968612 1108 58-59 0.7484135316474649 1108 60-61 0.7656216328164547 1108 62-63 0.6863233505592241 1108 64-65 0.6536095240409168 1108 66-67 0.6984161373309838 1108 68-69 0.5060929072319915 1108 70-71 0.5070975303573029 1108 72-73 0.44585684260413316 1108 74-75 0.4983097211897096 1108 76-77 0.5184439623479697 1108 78-79 0.4780184365030813 1108 80-81 0.7084490829916099 1108 82-83 0.8343136840056751 1108 84-85 0.888728175890698 1108 86-87 0.8343305391933953 1108 88-89 0.8153608056176473 1108 90-91 0.9685115016490791 1108 92-93 0.9255127692639427 1108 94-95 0.6779678602064649 1108 96-97 0.6545442080069819 1108 98-99 0.5695646921933033 1108 100-101 0.28177422061494717 1109 1 0.48275650919078217 1109 2 0.47938216467663963 1109 3 0.44282889941550607 1109 4 0.2815125871571098 1109 5 0.44093983895946565 1109 6 0.4197467952439382 1109 7 0.44220622110098873 1109 8 0.410994345373517 1109 9 0.35794171315173173 1109 10-11 0.4163628158904018 1109 12-13 0.4333824048787207 1109 14-15 0.4368035999153079 1109 16-17 0.5795678798090194 1109 18-19 0.5449432141275992 1109 20-21 0.2748703468164351 1109 22-23 0.4663022738537066 1109 24-25 0.4499196288910241 1109 26-27 0.4134964020556424 1109 28-29 0.4055592097690166 1109 30-31 0.4810718313062594 1109 32-33 0.24246935815605752 1109 34-35 0.4315245727012922 1109 36-37 0.42876746074932726 1109 38-39 0.39865827914890417 1109 40-41 0.4466977585471881 1109 42-43 0.39610826109491626 1109 44-45 0.38453702263321077 1109 46-47 0.47933465779805573 1109 48-49 0.4180203167253609 1109 50-51 0.34738725661404146 1109 52-53 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-1.4128897606567357 2316 74-75 -1.121724880757256 2316 76-77 -1.0783910979721476 2316 78-79 -1.203268330398494 2316 80-81 -1.140044593847854 2316 82-83 -1.0782822458796595 2316 84-85 -1.1482169611733255 2316 86-87 -1.3444934052046023 2316 88-89 -1.6703545999007474 2316 90-91 -1.561795809925215 2316 92-93 -1.7794668129744693 2316 94-95 -2.0106379463511104 2316 96-97 -2.0116940713766773 2316 98-99 -1.846107339579273 2316 100-101 -1.6578851349751158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9748.0 3 3106.0 4 3273.0 5 3802.0 6 4431.0 7 5725.0 8 7169.0 9 9316.0 10 11491.0 11 14742.0 12 19112.0 13 22950.0 14 26932.0 15 32662.0 16 38452.0 17 45929.0 18 41820.0 19 36049.0 20 37280.0 21 39443.0 22 44268.0 23 48970.0 24 54690.0 25 61519.0 26 69859.0 27 79248.0 28 90962.0 29 103596.0 30 121098.0 31 141187.0 32 167629.0 33 201533.0 34 246844.0 35 304676.0 36 361485.0 37 353146.0 38 162870.0 39 8201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.637328027641004 18.51812488488967 15.05497900453576 22.78956808293357 2 15.720134223763408 20.136366333304768 39.68418519714939 24.459314245782434 3 19.105981286600596 24.48679230605433 29.489788585920717 26.917437821424357 4 13.192469583338964 16.67639652599521 36.157135195378984 33.97399869528685 5 14.148548950881812 37.272236667164464 32.962226338673624 15.616988043280102 6 32.17856349278041 35.39370330540532 17.343207372627727 15.084525829186548 7 29.09521342371773 32.85636316176645 19.793767747957254 18.254655666558563 8 27.17908628217014 33.681286683657305 19.6000354621733 19.539591571999253 9 27.540717229695765 16.399118665407617 19.56599050439431 36.4941736005023 10-11 25.47879321630781 26.295146114907737 26.528980725660205 21.69707994312425 12-13 26.5933612837297 23.75668883878743 27.074992283455977 22.57495759402689 14-15 23.340210466645352 24.983751948612653 25.925183161350073 25.75085442339192 16-17 22.496757467464683 28.193760660479693 26.403731099920098 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22.7369108565239 25.723051797354202 28.50473318349409 23.035304162627813 44-45 22.443603355751687 26.10005477601552 28.5133459908487 22.942995877384096 46-47 22.77292840453515 26.465358824147717 27.81890894209342 22.942803829223713 48-49 22.796840024033767 26.190580140047608 27.941694222521264 23.07088561339736 50-51 23.06475833522107 26.597631746947016 27.428900924648364 22.90870899318355 52-53 23.137795343695505 26.5162232581281 27.322110073832317 23.02387132434407 54-55 22.60284613702889 26.592452993696135 27.646578429400385 23.158122439874585 56-57 22.627456194153815 26.669411353613377 27.670101808775055 23.033030643457753 58-59 22.74899139472876 26.143437442749047 27.65809170960076 23.44947945292143 60-61 22.662166343261525 26.439762456205212 27.82106321376075 23.077007986772514 62-63 22.437727206756563 26.623364321395698 27.7719780296719 23.166930442175833 64-65 22.22831960461285 26.355387149917625 27.70978583196046 23.706507413509062 66-67 22.344199121476382 26.459164994010067 27.67370807870074 23.522927805812806 68-69 22.490459903257168 26.27684410980267 27.759057922963237 23.473638063976928 70-71 22.435777872538427 26.490157011955002 27.435619694016832 23.638445421489738 72-73 22.515462300458143 26.52162250600483 27.34748680156025 23.615428391976774 74-75 22.31642234682159 26.71806802510368 27.35498572306536 23.610523905009366 76-77 22.491243389294365 26.308038288548037 27.328253455689737 23.872464866467865 78-79 22.63311199276853 26.45166094383764 27.433303355956124 23.481923707437712 80-81 22.536512509054713 26.8368774323345 27.185853543376876 23.44075651523391 82-83 22.547461342469266 26.591135760905342 27.113960927813686 23.747441968811707 84-85 22.58189879135713 26.30863275384943 27.212833235092454 23.896635219700983 86-87 22.661897822965738 26.38919069416495 27.004055219519753 23.944856263349564 88-89 22.54281091944155 26.287639680707215 27.145950787834288 24.02359861201695 90-91 22.477442863741597 26.26770751469491 27.174673056859653 24.080176564703834 92-93 22.57684218157732 25.744381987759297 27.28784110316283 24.390934727500557 94-95 22.381533282766704 25.87774927202676 27.31530588553036 24.425411559676174 96-97 22.52078124255871 25.744203556396787 27.224280539647378 24.510734661397123 98-99 22.506227367198655 25.713861086295374 26.91087656351486 24.869034982991106 100-101 22.891462731548526 25.15959422557875 27.170224164241525 24.7787188786312 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1288.0 1 889.0 2 590.0 3 688.0 4 603.5 5 622.0 6 1076.0 7 2369.0 8 3958.0 9 4526.0 10 3856.5 11 3225.5 12 3346.5 13 3678.5 14 4040.5 15 4450.5 16 4782.5 17 5159.5 18 5639.0 19 6149.5 20 6653.5 21 7451.0 22 8640.5 23 9976.0 24 11541.5 25 13605.0 26 16629.0 27 20411.0 28 23866.5 29 27368.0 30 31618.0 31 36470.0 32 42446.5 33 46083.5 34 47734.0 35 50256.0 36 54397.5 37 60377.0 38 65121.0 39 68553.5 40 73146.5 41 81529.5 42 90354.0 43 97102.0 44 100874.0 45 104449.5 46 107272.5 47 110735.5 48 115625.0 49 117574.5 50 118621.5 51 116417.0 52 113608.0 53 112946.5 54 112984.5 55 106778.5 56 95031.0 57 86269.0 58 79880.0 59 71387.5 60 59793.5 61 49989.5 62 42187.5 63 33733.0 64 27219.0 65 22535.5 66 17684.5 67 13803.5 68 10668.5 69 8054.5 70 6317.5 71 4990.0 72 4181.5 73 3497.5 74 2588.5 75 2004.0 76 1465.0 77 919.0 78 568.0 79 359.5 80 204.5 81 130.5 82 82.0 83 48.0 84 32.0 85 17.0 86 15.5 87 12.5 88 6.5 89 6.0 90 5.0 91 4.5 92 2.5 93 0.5 94 0.5 95 3.0 96 2.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18156880587952148 2 0.04853036673208767 3 0.047805541159714325 4 0.052582800613993154 5 0.06114892101476899 6 0.06388349021963202 7 0.03495636055855059 8 0.03274893722450451 9 0.038811114738899706 10-11 0.04055728543598093 12-13 0.03780624292265485 14-15 0.03304545677683906 16-17 0.03075566690047782 18-19 0.039568886928199105 20-21 0.050227117503779804 22-23 0.04615821031341128 24-25 0.06666747934988418 26-27 0.05161087541467436 28-29 0.04421435991477369 30-31 0.05329115287790346 32-33 0.04876099305057009 34-35 0.04806911409512281 36-37 0.04597700392031795 38-39 0.025451261575382026 40-41 0.023293258166725037 42-43 0.020674002121103197 44-45 0.00413480042422064 46-47 0.011267742988712817 48-49 0.010427604257098266 50-51 0.00907679296312977 52-53 0.009735725301650988 54-55 0.010790017043284935 56-57 0.008516700475386735 58-59 0.010839436968674027 60-61 0.010542917416339479 62-63 0.0039371207226642745 64-65 0.0070176294052509665 66-67 0.0039865406480533655 68-69 0.008549647092312797 70-71 0.021975393489682603 72-73 0.01891135811555894 74-75 0.0197020769217844 76-77 0.011959621944160097 78-79 0.004826679379667918 80-81 0.0063092771413406565 82-83 0.005403245175873983 84-85 0.005139672240465496 86-87 0.007758928286087336 88-89 0.029058916128785688 90-91 0.03573060605631302 92-93 0.02192597356429351 94-95 0.03072272028355176 96-97 0.0344786346131227 98-99 0.020558688961861984 100-101 0.12437347889587978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3035213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.0326601227719 #Duplication Level Percentage of deduplicated Percentage of total 1 69.90036427409922 30.07998618265259 2 14.298436409271115 12.30599508574462 3 6.050736910942773 7.811379149427335 4 3.0707385870866393 5.285681997759206 5 1.7773763163906247 3.824261546675103 6 1.099965239294526 2.8400658173654887 7 0.7437314539653206 2.2403320016773236 8 0.5274497223395516 1.8158051706630665 9 0.3945648861227158 1.5281258976809127 >10 1.9114863464421656 14.553894967529363 >50 0.12494523511082471 3.746618861734812 >100 0.08692457994320302 7.439444558248209 >500 0.009496772069496288 2.8094328932722035 >1k 0.003551638334835825 2.5874877943258148 >5k 7.72095290181701E-5 0.26517123266836584 >10k+ 1.544190580363402E-4 0.8663168425754881 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14852 0.4893231545858561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11222 0.36972693514425514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7981 0.2629469496868918 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3441 0.11336930884257547 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3304 0.10885562232370513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2946616926060875E-5 2 0.0 0.0 0.0 0.0 3.2946616926060875E-5 3 0.0 0.0 0.0 0.0 3.2946616926060875E-5 4 0.0 0.0 0.0 0.0 3.2946616926060875E-5 5 0.0 0.0 0.0 0.0 3.2946616926060875E-5 6 0.0 0.0 0.0 3.2946616926060875E-5 3.2946616926060875E-5 7 0.0 0.0 0.0 3.2946616926060875E-5 3.2946616926060875E-5 8 0.0 0.0 0.0 3.2946616926060875E-5 3.2946616926060875E-5 9 0.0 0.0 0.0 6.589323385212175E-5 3.2946616926060875E-5 10-11 0.0 0.0 0.0 6.589323385212175E-5 3.2946616926060875E-5 12-13 0.0 0.0 0.0 8.236654231515218E-5 1.6473308463030436E-4 14-15 0.0 0.0 0.0 9.883985077818262E-5 1.6473308463030436E-4 16-17 0.0 0.0 0.0 1.317864677042435E-4 1.6473308463030436E-4 18-19 0.0 0.0 0.0 1.4825977616727393E-4 1.6473308463030436E-4 20-21 0.0 0.0 0.0 2.1415301001939568E-4 2.306263184824261E-4 22-23 0.0 0.0 0.0 6.259857215951566E-4 2.63572935408487E-4 24-25 0.0 0.0 0.0 0.0011037116670230393 2.8004624387151743E-4 26-27 0.0 0.0 0.0 0.0023227364932872914 2.9651955233454786E-4 28-29 0.0 0.0 0.0 0.006770529778305509 2.9651955233454786E-4 30-31 0.0 0.0 0.0 0.0193231908271347 2.9651955233454786E-4 32-33 0.0 0.0 0.0 0.04297886178004641 3.129928607975783E-4 34-35 0.0 0.0 0.0 0.07884125430406366 3.294661692606087E-4 36-37 0.0 0.0 0.0 0.13592126812846414 3.294661692606087E-4 38-39 0.0 0.0 0.0 0.2212530059669618 3.294661692606087E-4 40-41 0.0 0.0 0.0 0.3159910029378498 3.294661692606087E-4 42-43 0.0 0.0 0.0 0.4128705300089318 3.294661692606087E-4 44-45 0.0 0.0 0.0 0.5278542230808843 3.294661692606087E-4 46-47 0.0 0.0 0.0 0.6682727044197557 3.294661692606087E-4 48-49 0.0 0.0 0.0 0.8174714591694223 3.294661692606087E-4 50-51 0.0 0.0 0.0 0.9770648715592612 3.294661692606087E-4 52-53 0.0 0.0 0.0 1.1371195365860651 3.624127861866696E-4 54-55 0.0 0.0 0.0 1.3096444961193827 3.953594031127305E-4 56-57 0.0 0.0 0.0 1.5086914822781794 3.953594031127305E-4 58-59 0.0 0.0 0.0 1.7238987840392093 3.953594031127305E-4 60-61 0.0 0.0 0.0 1.9605049134937151 3.953594031127305E-4 62-63 0.0 0.0 0.0 2.192613829737814 4.2830602003879136E-4 64-65 0.0 0.0 0.0 2.4419538266342427 4.2830602003879136E-4 66-67 0.0 0.0 0.0 2.7233673551081914 4.2830602003879136E-4 68-69 0.0 0.0 0.0 3.0320112624715296 4.612526369648522E-4 70-71 0.0 0.0 0.0 3.3612962253390455 4.777259454278827E-4 72-73 0.0 0.0 0.0 3.7022113439814603 5.436191792800044E-4 74-75 0.0 0.0 0.0 4.047788408918913 5.930391046690957E-4 76-77 0.0 0.0 0.0 4.420497012894977 5.930391046690957E-4 78-79 0.0 1.6473308463030437E-5 0.0 4.832296777853811 6.095124131321262E-4 80-81 3.2946616926060875E-5 3.2946616926060875E-5 0.0 5.270964508915849 6.589323385212174E-4 82-83 3.2946616926060875E-5 3.2946616926060875E-5 0.0 5.725215989783913 7.248255723733392E-4 84-85 3.2946616926060875E-5 3.2946616926060875E-5 0.0 6.190076281302169 7.248255723733392E-4 86-87 3.2946616926060875E-5 3.2946616926060875E-5 0.0 6.660092718369353 7.577721892994E-4 88-89 3.2946616926060875E-5 3.2946616926060875E-5 0.0 7.159761110669992 7.90718806225461E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7265 0.0 55.054943 1 GTATCAA 13640 0.0 43.84579 1 TATCAAC 17890 0.0 33.2578 2 ATCAACG 18240 0.0 32.593582 3 TCAACGC 18465 0.0 32.247894 4 CAACGCA 18715 0.0 31.5865 5 AACGCAG 19445 0.0 30.44454 6 GTGGTAT 3400 0.0 26.889019 1 TGGTATC 3275 0.0 26.84479 2 ACGCAGA 22315 0.0 26.37559 7 CGCAGAG 22570 0.0 25.993832 8 GTACATG 15545 0.0 24.259792 1 GCAGAGT 23825 0.0 23.946663 9 TACATGG 15635 0.0 23.67768 2 GAGTACT 13315 0.0 23.063948 12-13 AGTACTT 13725 0.0 21.994267 12-13 ACATGGG 16300 0.0 21.953661 3 GTACTTT 14115 0.0 21.505941 14-15 AGAGTAC 22200 0.0 20.788233 10-11 GTGTAAG 1055 0.0 20.761465 1 >>END_MODULE