FastQCFastQC Report
Thu 26 May 2016
SRR938626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035213
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143350.4722897536350826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116560.38402576689016554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86420.28472466347501807No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG37110.1222648954126119No Hit
ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT33970.11191965769782879No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC30820.10154147336611961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.055.9296261
GTATCAA135400.042.7875561
TATCAAC179450.032.2578932
ATCAACG181500.031.7478453
TCAACGC186050.030.9453644
CAACGCA188800.030.4946235
AACGCAG194300.029.6510226
TGGTATC30850.027.1360322
ACGCAGA220500.026.0632427
GTGGTAT32950.025.9839951
CGCAGAG223650.025.6749178
GTACATG150800.024.5080381
TACATGG152400.024.1571042
GCAGAGT238800.023.5687039
GAGTACT135850.023.12713612-13
ACATGGG157000.022.2877623
TAAGGTG16350.022.0992225
AGTACTT140350.021.79340612-13
GTACTTT143550.021.75425714-15
AGAGTAC221300.021.20445810-11
Produced by FastQC (version 0.11.5)