##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3035213 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806240945857837 31.0 31.0 34.0 30.0 34.0 2 32.02541403189826 31.0 31.0 34.0 30.0 34.0 3 32.081301378189934 33.0 31.0 34.0 30.0 34.0 4 35.76041813210473 37.0 35.0 37.0 35.0 37.0 5 35.62724065823387 37.0 35.0 37.0 33.0 37.0 6 35.66435106860705 37.0 35.0 37.0 33.0 37.0 7 35.601897132095836 37.0 35.0 37.0 33.0 37.0 8 35.59566857416596 37.0 35.0 37.0 33.0 37.0 9 37.165078694641856 39.0 37.0 39.0 33.0 39.0 10-11 37.074491477204404 39.0 37.0 39.0 33.0 39.0 12-13 36.96610534417189 39.0 37.0 39.0 33.0 39.0 14-15 37.99591412530191 40.0 37.0 41.0 33.0 41.0 16-17 37.93541277004283 40.0 37.0 41.0 33.0 41.0 18-19 38.066825787844216 40.0 37.0 41.0 33.0 41.0 20-21 38.117160476052256 40.0 37.0 41.0 33.0 41.0 22-23 38.025600509750056 40.0 37.0 41.0 33.0 41.0 24-25 37.92500411009046 40.0 37.0 41.0 33.0 41.0 26-27 37.72832203209462 40.0 37.0 41.0 32.0 41.0 28-29 37.59937506857015 39.5 36.5 41.0 32.0 41.0 30-31 37.336962348276714 39.0 36.0 41.0 31.5 41.0 32-33 37.09641283824233 39.0 36.0 41.0 31.0 41.0 34-35 36.922161805448255 39.0 35.0 41.0 31.0 41.0 36-37 36.90455612176147 39.0 35.0 41.0 31.0 41.0 38-39 36.770473933789816 39.0 35.0 41.0 30.0 41.0 40-41 36.57208868043198 39.0 35.0 41.0 30.0 41.0 42-43 36.322824131288314 39.0 35.0 40.5 30.0 41.0 44-45 35.993740966449465 39.0 35.0 40.0 29.5 41.0 46-47 35.54928747998905 38.0 35.0 40.0 27.5 41.0 48-49 35.13524092048894 38.0 34.0 40.0 26.5 41.0 50-51 34.748825206006956 38.0 34.0 40.0 25.5 41.0 52-53 34.26647223769798 38.0 33.0 40.0 24.0 41.0 54-55 33.9453241008127 37.5 33.0 40.0 23.0 41.0 56-57 33.60805007753986 37.0 33.0 40.0 23.0 41.0 58-59 33.17475396290145 36.5 32.0 39.5 21.5 41.0 60-61 32.81317192566057 36.0 31.5 39.0 20.5 40.5 62-63 32.357105415666055 35.5 31.0 39.0 18.5 40.0 64-65 31.867577992055253 35.0 31.0 38.5 17.5 40.0 66-67 31.381773041957846 35.0 30.0 38.0 13.5 40.0 68-69 30.85781623892623 34.5 29.5 37.5 10.0 39.5 70-71 30.300886132208845 34.0 29.0 37.0 8.0 39.0 72-73 29.875969989585574 34.0 29.0 36.0 7.0 39.0 74-75 29.385253687303 34.0 28.0 36.0 4.0 38.5 76-77 28.126849911357127 31.5 26.5 34.5 2.0 36.5 78-79 28.81664878214478 33.5 28.0 35.0 2.0 37.0 80-81 28.564754269304988 33.0 28.0 35.0 2.0 37.0 82-83 28.12258892539008 33.0 27.0 35.0 2.0 36.0 84-85 27.635963604531213 33.0 26.0 35.0 2.0 36.0 86-87 27.09031474891548 32.5 25.5 35.0 2.0 35.0 88-89 26.49255867710108 32.0 25.0 34.5 2.0 35.0 90-91 25.986890705858205 32.0 24.0 34.0 2.0 35.0 92-93 25.29237915098545 31.0 20.5 34.0 2.0 35.0 94-95 24.57014993675897 31.0 18.5 34.0 2.0 35.0 96-97 23.61966491313789 31.0 4.5 34.0 2.0 35.0 98-99 22.436479087299638 30.5 2.0 34.0 2.0 35.0 100-101 18.83292852923337 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3383074723602668 1101 2 0.38061288240001545 1101 3 0.3655377715345409 1101 4 0.260888005943265 1101 5 0.17146297780183062 1101 6 0.1473386062359765 1101 7 0.27970288404062416 1101 8 0.26993566572609495 1101 9 0.26369105677527216 1101 10-11 0.39943860692211786 1101 12-13 0.4244516774218212 1101 14-15 0.5500220340422217 1101 16-17 0.4868704717412484 1101 18-19 0.5755092749067003 1101 20-21 0.5957056903014717 1101 22-23 0.5403188847356688 1101 24-25 0.5113747710884482 1101 26-27 0.5825237572245925 1101 28-29 0.5866140456369138 1101 30-31 0.5108251433120827 1101 32-33 0.5202558573339218 1101 34-35 0.4483849847670953 1101 36-37 0.6268059989334773 1101 38-39 0.6658969923157585 1101 40-41 0.870336927049479 1101 42-43 0.9559478654340197 1101 44-45 0.8584838431597817 1101 46-47 0.9092652955843121 1101 48-49 0.9192680366549553 1101 50-51 1.0045868246474683 1101 52-53 1.016421113890253 1101 54-55 0.9928178813540356 1101 56-57 1.1031254081302961 1101 58-59 1.1417013501077449 1101 60-61 1.1272076239674576 1101 62-63 1.1008455674622653 1101 64-65 1.0547624803555955 1101 66-67 1.0506545613587015 1101 68-69 1.077101451680356 1101 70-71 1.0116319938510792 1101 72-73 0.9077690341304674 1101 74-75 0.9172863329240251 1101 76-77 0.9199319131979955 1101 78-79 0.9679852015388555 1101 80-81 1.0940160911776537 1101 82-83 0.9577972500668324 1101 84-85 1.006817609873007 1101 86-87 1.085853733634913 1101 88-89 1.192681367124365 1101 90-91 1.378586812359483 1101 92-93 1.3805082958562274 1101 94-95 1.536427791387581 1101 96-97 1.3437828632663873 1101 98-99 1.2604891363657629 1101 100-101 1.0903758416180303 1102 1 0.2897459322755829 1102 2 0.29386064849125404 1102 3 0.30766181123434677 1102 4 0.2820645347856683 1102 5 0.19773086185938382 1102 6 0.23811269909012367 1102 7 0.20934686997153307 1102 8 0.2689434597272893 1102 9 0.3493533093505832 1102 10-11 0.3148361736233056 1102 12-13 0.41883110378222455 1102 14-15 0.45975598333502177 1102 16-17 0.2880102079011664 1102 18-19 0.3589152296759366 1102 20-21 0.38877726473696583 1102 22-23 0.4658872855572369 1102 24-25 0.4856259885987342 1102 26-27 0.47590541017317634 1102 28-29 0.41270685152133524 1102 30-31 0.4465552039616938 1102 32-33 0.4077212856470709 1102 34-35 0.38868497407213454 1102 36-37 0.5093812780864582 1102 38-39 0.5292146778252871 1102 40-41 0.6292393524181819 1102 42-43 0.5582053445873285 1102 44-45 0.6001358341006053 1102 46-47 0.43701451366440125 1102 48-49 0.5350555233475234 1102 50-51 0.5268731915108376 1102 52-53 0.4099894574039098 1102 54-55 0.39276832760795344 1102 56-57 0.35609891566458884 1102 58-59 0.4442376523426148 1102 60-61 0.5072176201758296 1102 62-63 0.4812164401146717 1102 64-65 0.4806861022355129 1102 66-67 0.5290176841219214 1102 68-69 0.5387809353361028 1102 70-71 0.5031387984739695 1102 72-73 0.33330029791939353 1102 74-75 0.3640504280616881 1102 76-77 0.4225816499020887 1102 78-79 0.5384194484431966 1102 80-81 0.4126407089207085 1102 82-83 0.4527203475039734 1102 84-85 0.33261727826632637 1102 86-87 0.21942395222311006 1102 88-89 0.3305461599229744 1102 90-91 0.11251386064905944 1102 92-93 0.21306137447853146 1102 94-95 0.07705727602987977 1102 96-97 0.3080136799153941 1102 98-99 0.19080014361885844 1102 100-101 0.23066679487350683 1103 1 0.25486338117709906 1103 2 0.21129358201606152 1103 3 0.21390676529413355 1103 4 0.16737029146278104 1103 5 0.23919501961745482 1103 6 0.1434912282945433 1103 7 0.24525337771726186 1103 8 0.2256358219773702 1103 9 0.265448654751566 1103 10-11 0.3234316810314226 1103 12-13 0.289996592003682 1103 14-15 0.41484850283048047 1103 16-17 0.43661961390964166 1103 18-19 0.3467600408114251 1103 20-21 0.3739110969448447 1103 22-23 0.3303347589083998 1103 24-25 0.4810171096057019 1103 26-27 0.49393975711618765 1103 28-29 0.5188723594342548 1103 30-31 0.5192648975730947 1103 32-33 0.441098513066045 1103 34-35 0.4607276637556268 1103 36-37 0.4591823404951825 1103 38-39 0.5251747034170435 1103 40-41 0.5759676498181534 1103 42-43 0.6257193532809282 1103 44-45 0.6135065239502211 1103 46-47 0.7046397254820036 1103 48-49 0.7274831133223358 1103 50-51 0.7123577467717368 1103 52-53 0.8535735634485846 1103 54-55 0.8523401203949277 1103 56-57 0.8921455909024161 1103 58-59 0.8841582491589719 1103 60-61 0.7708966606067307 1103 62-63 0.7608479016480914 1103 64-65 0.7953017944785366 1103 66-67 0.8942234582349364 1103 68-69 0.8428455777908503 1103 70-71 0.6528464516906389 1103 72-73 0.5694988123229905 1103 74-75 0.6759551413770986 1103 76-77 0.48946946972295535 1103 78-79 0.5988289523361985 1103 80-81 0.5023586446670727 1103 82-83 0.5033598370723276 1103 84-85 0.4744341998301742 1103 86-87 0.7143714780252033 1103 88-89 0.5755687818463144 1103 90-91 0.43842834165161904 1103 92-93 0.38183055352666173 1103 94-95 0.4129895259025105 1103 96-97 0.3226764884852642 1103 98-99 0.2613416269310491 1103 100-101 0.03078598199513749 1104 1 0.28279665993392555 1104 2 0.2619856242792764 1104 3 0.2755260515767546 1104 4 0.16897389883011016 1104 5 0.1493312648394749 1104 6 0.13165666676449206 1104 7 0.15808904181892558 1104 8 0.19870506541224842 1104 9 0.3105138186940266 1104 10-11 0.3118911273300071 1104 12-13 0.3343971258709715 1104 14-15 0.5417402311097845 1104 16-17 0.4871204017987978 1104 18-19 0.4282962590381274 1104 20-21 0.3655059882954603 1104 22-23 0.3748095411600829 1104 24-25 0.3843739365249448 1104 26-27 0.4471909913406549 1104 28-29 0.40579353469041735 1104 30-31 0.5025815447368913 1104 32-33 0.40343678059323196 1104 34-35 0.2983005911046561 1104 36-37 0.44558564735373096 1104 38-39 0.4150390553368126 1104 40-41 0.49402430482816584 1104 42-43 0.4267301340562071 1104 44-45 0.42109200479695374 1104 46-47 0.3107857340849094 1104 48-49 0.37378189332787315 1104 50-51 0.4339684432260853 1104 52-53 0.4551318331074512 1104 54-55 0.37727022663884924 1104 56-57 0.3700812018922548 1104 58-59 0.45332343592802005 1104 60-61 0.4787611531364817 1104 62-63 0.428503457646805 1104 64-65 0.4555980286320285 1104 66-67 0.31865747485620943 1104 68-69 0.311670213472393 1104 70-71 0.21725253108976617 1104 72-73 0.1677478461363151 1104 74-75 0.2880594419922424 1104 76-77 0.3378122998640549 1104 78-79 0.40852848410943565 1104 80-81 0.6613240413145611 1104 82-83 0.2607533537349518 1104 84-85 0.44580412420949145 1104 86-87 0.5238509686835435 1104 88-89 0.25970539481754074 1104 90-91 0.3619549844281096 1104 92-93 0.3688636196334123 1104 94-95 0.518522168489266 1104 96-97 0.26390235747866697 1104 98-99 0.11907208929680735 1104 100-101 -0.23149930891544201 1105 1 0.22540775684513292 1105 2 0.27597122549997977 1105 3 0.20092121671836338 1105 4 0.17787667054049194 1105 5 0.09495501590509292 1105 6 0.16389709034218924 1105 7 0.20042099446402517 1105 8 0.20334236533368255 1105 9 0.2373390920375158 1105 10-11 0.26853447805531516 1105 12-13 0.3168589644780653 1105 14-15 0.4245126013764704 1105 16-17 0.3201979046649086 1105 18-19 0.307052435552265 1105 20-21 0.3559333153385964 1105 22-23 0.40750888485290204 1105 24-25 0.4286397489688838 1105 26-27 0.3599262173588116 1105 28-29 0.3465223521098437 1105 30-31 0.4799664456652053 1105 32-33 0.41732843388187035 1105 34-35 0.44676006997965345 1105 36-37 0.47164950323824684 1105 38-39 0.42734693074449837 1105 40-41 0.3998296121471725 1105 42-43 0.44285795166396014 1105 44-45 0.5164735801613034 1105 46-47 0.18788780567430052 1105 48-49 0.1129113570062259 1105 50-51 0.24879460527201758 1105 52-53 0.25443880374405836 1105 54-55 0.23706959584864506 1105 56-57 0.3131550753901351 1105 58-59 0.3396154256723847 1105 60-61 0.2900968870519449 1105 62-63 0.4621145354898957 1105 64-65 0.43859436348430236 1105 66-67 0.30051797171114814 1105 68-69 0.4390990139019202 1105 70-71 0.3679400328439293 1105 72-73 0.5155011515073724 1105 74-75 0.5429307011880553 1105 76-77 0.33873608548794465 1105 78-79 0.4787829512425539 1105 80-81 0.5027082767872848 1105 82-83 0.5130875902682028 1105 84-85 0.5614162672957228 1105 86-87 0.7108372947066499 1105 88-89 0.6257527300064076 1105 90-91 0.4896267961277445 1105 92-93 0.6414451888274009 1105 94-95 0.44949014927718167 1105 96-97 0.31388421063751437 1105 98-99 0.30863978740535813 1105 100-101 -0.09082647460810023 1106 1 0.1423568745554995 1106 2 0.1346796724634487 1106 3 0.22696268034796674 1106 4 0.11867413375653513 1106 5 0.07165720099354189 1106 6 -0.04540591271374694 1106 7 0.060248511120832404 1106 8 0.11420301933479493 1106 9 -0.004033774696154069 1106 10-11 0.08098214745814403 1106 12-13 0.09904429445320062 1106 14-15 0.22275214288417544 1106 16-17 0.13068333557241374 1106 18-19 0.2255766088983151 1106 20-21 0.17317145896257102 1106 22-23 0.2440991269550068 1106 24-25 0.2187861093864356 1106 26-27 0.13432435193192305 1106 28-29 0.19160996030917943 1106 30-31 0.35546125582590093 1106 32-33 0.3081995412905272 1106 34-35 0.19822882246906204 1106 36-37 -0.00751428559868117 1106 38-39 0.28559292989026375 1106 40-41 0.4304552466821434 1106 42-43 0.5609014250427293 1106 44-45 0.4933601405622525 1106 46-47 0.2373238004810716 1106 48-49 0.3558080081022439 1106 50-51 0.45103265991279073 1106 52-53 0.47309997944096693 1106 54-55 0.17072176093706304 1106 56-57 0.4434054020856024 1106 58-59 0.4575954455537712 1106 60-61 0.2880540869794075 1106 62-63 0.40322916026044453 1106 64-65 0.059595416028464854 1106 66-67 0.17782798621965057 1106 68-69 -0.36111277671128406 1106 70-71 -0.12683638838729294 1106 72-73 0.036963476019785446 1106 74-75 0.3341327837339563 1106 76-77 0.15258100302170519 1106 78-79 0.12061454756728907 1106 80-81 -0.5923891301574713 1106 82-83 -0.049362515579435495 1106 84-85 -0.0369297150703467 1106 86-87 0.027769982304423735 1106 88-89 0.0892942995683903 1106 90-91 0.13789055377818116 1106 92-93 0.03302148810926653 1106 94-95 0.2723113532114283 1106 96-97 0.14323879428934916 1106 98-99 -0.10302812431991626 1106 100-101 -0.5526814947013534 1107 1 0.17015878558286346 1107 2 0.173833402259433 1107 3 0.11726076084453041 1107 4 0.0561977689465607 1107 5 0.025616258324362207 1107 6 0.11999436960451959 1107 7 0.022566683158935064 1107 8 0.04110426662082034 1107 9 0.12438354777911798 1107 10-11 0.17240509999868436 1107 12-13 0.14675274052910225 1107 14-15 0.30515276913521205 1107 16-17 0.32219092654344905 1107 18-19 0.22373123031147202 1107 20-21 0.2715007770302904 1107 22-23 0.23207553903535683 1107 24-25 0.21831544310227713 1107 26-27 0.28072071895871176 1107 28-29 0.2941025355994782 1107 30-31 0.26306681547460187 1107 32-33 0.32568102434164814 1107 34-35 0.3119381291918941 1107 36-37 0.30298670707929176 1107 38-39 0.3073766287811921 1107 40-41 0.12146242362985049 1107 42-43 0.23381586402481958 1107 44-45 0.22484054291468425 1107 46-47 0.28234516995288317 1107 48-49 0.3337174592442267 1107 50-51 0.40134415541721324 1107 52-53 0.4519546344323899 1107 54-55 0.3433505401224508 1107 56-57 0.36554823361397126 1107 58-59 0.34291485477181993 1107 60-61 0.33372460917040314 1107 62-63 0.35760876097243255 1107 64-65 0.44850187283637766 1107 66-67 0.473479152005023 1107 68-69 0.38088936023589426 1107 70-71 0.24789323318886147 1107 72-73 0.2336987227711731 1107 74-75 0.2781153458537595 1107 76-77 0.2879743740844063 1107 78-79 0.4193486793897101 1107 80-81 0.5648566040982281 1107 82-83 0.5641956074278767 1107 84-85 0.5907058123692934 1107 86-87 0.5946565513224407 1107 88-89 0.4865615010966913 1107 90-91 0.4035474409095521 1107 92-93 0.25363968687958405 1107 94-95 0.010392289882158678 1107 96-97 0.3411028251161561 1107 98-99 0.21738660806453325 1107 100-101 -0.30691708375549 1108 1 0.16647926081936149 1108 2 0.18418781853999633 1108 3 0.1838279042431239 1108 4 0.1324212476715303 1108 5 0.11902761171189269 1108 6 0.04971985555395264 1108 7 0.12348791796939196 1108 8 0.134847063430918 1108 9 0.1966393741445316 1108 10-11 0.18871563579998707 1108 12-13 0.20081862699523612 1108 14-15 0.4160472796276977 1108 16-17 0.2338249000743815 1108 18-19 0.34426970017990044 1108 20-21 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-0.8935050385414058 2316 64-65 -0.8309572168752801 2316 66-67 -0.7042915201738289 2316 68-69 -0.6344606044398411 2316 70-71 -0.8934210390115389 2316 72-73 -0.8769015876632587 2316 74-75 -0.6284966904404286 2316 76-77 -0.5531437062513547 2316 78-79 -0.4851869337557062 2316 80-81 -0.7790687075336287 2316 82-83 -0.6303003931468929 2316 84-85 -0.6481296244541568 2316 86-87 -0.551633240863115 2316 88-89 -0.33043485870788913 2316 90-91 -0.4477409592604822 2316 92-93 -0.383390582722388 2316 94-95 -0.3184015885424998 2316 96-97 0.012480890543880463 2316 98-99 -0.10317679150678671 2316 100-101 -0.18306025779035906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 61.0 5 117.0 6 282.0 7 667.0 8 1475.0 9 2647.0 10 4532.0 11 6922.0 12 9783.0 13 13697.0 14 17720.0 15 23489.0 16 29938.0 17 38809.0 18 35036.0 19 26727.0 20 25226.0 21 26428.0 22 28540.0 23 32266.0 24 37510.0 25 44092.0 26 52650.0 27 63171.0 28 75895.0 29 90617.0 30 108530.0 31 129411.0 32 156386.0 33 193194.0 34 248179.0 35 325140.0 36 432221.0 37 497415.0 38 246494.0 39 9925.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55194722185981 19.275526193366098 15.641230418068552 22.531296166705545 2 16.215205077764473 20.875437079072416 38.6882881267437 24.221069716419404 3 19.091686338724 24.603903880653075 29.935704083935576 26.36870569668735 4 13.655120744409041 17.411002127363055 35.79135961792467 33.14251751030323 5 14.102143864337563 37.32306490950605 33.27329422767198 15.301496998484405 6 32.21154495582353 35.91803936000538 16.95880981005287 14.911605874118225 7 29.067317516101838 32.908300010575864 19.759107515683414 18.265274957638887 8 27.195554315298466 34.16564175232513 19.32931889788295 19.30948503449346 9 27.19324805211364 16.46734512536682 19.596252388217895 36.743154434301644 10-11 25.46824555640741 26.542272321580068 26.476955653524154 21.512526468488375 12-13 26.48390738969555 23.81702700930709 27.136728130776984 22.562337470220374 14-15 23.16694083743052 25.110264090197294 25.948541337955522 25.774253734416664 16-17 22.432247753287825 28.35886641234075 26.437567972989047 22.771317861382382 18-19 22.46185028859589 26.4105846937266 29.413751193079364 21.71381382459814 20-21 23.23033012839626 25.461425606703713 29.565799830193136 21.74244443470689 22-23 23.067376161079963 25.25745310131447 29.418594345767495 22.256576391838067 24-25 22.853568612217984 25.62120567414441 29.412496316148506 22.1127293974891 26-27 22.1775064444592 26.14540202436827 29.35922133041447 22.317870200758065 28-29 22.296903050955567 26.231651617201162 29.455247457097737 22.016197874745526 30-31 22.359798142667415 25.866998461063524 29.24625059262727 22.526952803641787 32-33 22.354526683959246 25.892301462862736 29.045012656442893 22.708159196735124 34-35 23.08119261173033 26.043594573693728 28.746170820374452 22.129041994201494 36-37 23.086785521606807 25.56407500298579 28.9887446101385 22.36039486526891 38-39 22.48219462335531 25.966386374655144 28.80737212524692 22.744046876742626 40-41 22.760271729425074 25.731381532863406 28.828270640820435 22.680076096891085 42-43 22.805617019482053 25.834144920736797 28.54071752672967 22.819520533051485 44-45 22.526577628419755 26.15279819781976 28.611802303792565 22.708821869967917 46-47 22.881194828830793 26.476560294121036 27.86092771742873 22.78131715961944 48-49 22.79115172477187 26.210203369582302 28.047438515847155 22.951206389798674 50-51 23.132231576498917 26.55428136344962 27.541576159564418 22.77191090048705 52-53 23.27301247062397 26.53255306958688 27.317605057701055 22.87682940208809 54-55 22.765074477474894 26.62111357588413 27.59285756880983 23.020954377831142 56-57 22.71461673365263 26.70715366598654 27.656395119551743 22.92183448080909 58-59 22.891029393983224 26.256559259597267 27.532944145929793 23.31946720048972 60-61 22.733066839131226 26.560837740217902 27.761165361376616 22.944930059274256 62-63 22.453811314065934 26.79912414713564 27.668783047515937 23.07828149128249 64-65 22.247894958278053 26.492654716489415 27.60127542943444 23.658174895798087 66-67 22.37059748264494 26.57220201768609 27.571029281504288 23.486171218164678 68-69 22.547479823299017 26.41777236569513 27.616266427340154 23.4184813836657 70-71 22.52904165742787 26.624838684914288 27.236608747536 23.609510910121838 72-73 22.63106411312814 26.694485691778468 27.096582678052577 23.577867517040815 74-75 22.453827787374394 26.930054002799803 26.982043764309125 23.634074445516674 76-77 22.50692126055074 26.567575323379284 27.212109990303812 23.713393425766167 78-79 22.662857598461787 26.62676392068695 27.35333566375737 23.35704281709389 80-81 22.630856973791786 27.034129339186265 27.038923132755627 23.296090554266318 82-83 22.63776064248983 26.868909775724237 26.940915105205864 23.552414476580065 84-85 22.61664925069591 26.593223603107624 26.997763957391797 23.792363188804664 86-87 22.69295568053267 26.724557585358273 26.772001956421356 23.810484777687705 88-89 22.5476820384751 26.73424573587989 26.75757196371915 23.960500261925862 90-91 22.44992969771252 26.630424128305176 27.0161930014011 23.9034531725812 92-93 22.4814064113369 26.20877270783405 27.058045207114162 24.25177567371489 94-95 22.30464372987578 26.35681670895368 27.01250423776139 24.326035323409148 96-97 22.39009255693093 26.283130706148132 26.95085979138861 24.37591694553232 98-99 22.370817053575518 26.317165429355654 26.620834597660593 24.691182919408238 100-101 22.455725917048643 26.141299826618397 26.852469143429005 24.550505112903956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1212.0 1 840.0 2 580.0 3 702.5 4 620.0 5 637.0 6 1088.5 7 2489.5 8 3899.5 9 4284.0 10 3765.0 11 3137.0 12 3258.5 13 3602.0 14 3936.5 15 4334.5 16 4638.5 17 5024.5 18 5494.0 19 5907.0 20 6391.5 21 7232.0 22 8422.5 23 9657.0 24 11089.5 25 13104.5 26 16077.5 27 20154.0 28 23754.0 29 27355.5 30 31843.0 31 36870.5 32 43337.5 33 46722.0 34 47893.5 35 50212.0 36 54456.0 37 60681.5 38 64988.0 39 68382.0 40 73040.0 41 82042.5 42 91752.0 43 98537.5 44 102536.0 45 106417.5 46 108575.0 47 112816.0 48 118638.0 49 120126.0 50 121606.0 51 118817.0 52 114399.0 53 114112.0 54 115617.0 55 108644.0 56 95496.0 57 85952.5 58 78639.0 59 69807.0 60 57967.0 61 47922.5 62 40618.5 63 32207.5 64 25094.5 65 19905.5 66 15050.5 67 12092.0 68 9420.5 69 6737.0 70 5233.0 71 4270.5 72 3619.5 73 3001.0 74 2356.0 75 1829.5 76 1224.0 77 710.0 78 399.5 79 228.0 80 126.0 81 67.5 82 43.5 83 24.0 84 9.0 85 6.0 86 5.0 87 4.0 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.600924877430349E-4 2 0.047344288522749474 3 9.225052739297044E-4 4 0.0 5 0.0904384634620371 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.1415301001939565E-4 26-27 0.0079566079876437 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009686305376261896 36-37 1.6473308463030437E-5 38-39 0.003442921468773361 40-41 0.004447793285018218 42-43 2.9651955233454786E-4 44-45 1.6473308463030437E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1531315924121306E-4 68-69 9.554518908557654E-4 70-71 1.317864677042435E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012684447516533436 82-83 6.918789554472783E-4 84-85 0.005386771867410952 86-87 0.0026192560456218396 88-89 9.883985077818261E-5 90-91 0.003475868085699422 92-93 8.895586570036436E-4 94-95 6.589323385212175E-5 96-97 0.0 98-99 6.589323385212175E-5 100-101 1.6473308463030437E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3035213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.46286628011243 #Duplication Level Percentage of deduplicated Percentage of total 1 67.28527873363257 27.225552360190676 2 15.438099641306996 12.493395228105134 3 6.470586634821579 7.854554452760048 4 3.3306176303597077 5.390653432297192 5 2.004986561095627 4.056375155751741 6 1.242649500677577 3.0168696347379167 7 0.8210678342738836 2.3255930589587965 8 0.5937246616595435 1.9219041273548085 9 0.43537268877160806 1.5854784199000727 >10 2.1212635397712285 15.25994318292216 >50 0.14302287575790132 3.9968042999599844 >100 0.09834940158945872 7.852226726475619 >500 0.010095417059805052 2.8384750149529383 >1k 0.004640635261287924 3.0412702912735035 >5k 8.141465370680569E-5 0.2846908346748876 >10k+ 1.6282930741361138E-4 0.8562137796846798 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14335 0.4722897536350826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11656 0.38402576689016554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8642 0.28472466347501807 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3711 0.1222648954126119 No Hit ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT 3397 0.11191965769782879 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3082 0.10154147336611961 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.2946616926060875E-5 0.0 10-11 0.0 0.0 0.0 3.2946616926060875E-5 0.0 12-13 0.0 0.0 0.0 4.941992538909131E-5 6.589323385212175E-5 14-15 0.0 0.0 0.0 9.883985077818262E-5 8.236654231515218E-5 16-17 0.0 0.0 0.0 1.317864677042435E-4 9.883985077818262E-5 18-19 0.0 0.0 0.0 1.4825977616727393E-4 1.317864677042435E-4 20-21 0.0 0.0 0.0 2.1415301001939568E-4 1.317864677042435E-4 22-23 0.0 0.0 0.0 6.259857215951566E-4 1.317864677042435E-4 24-25 0.0 0.0 0.0 0.0011037116670230393 1.317864677042435E-4 26-27 0.0 0.0 0.0 0.0023227364932872914 1.812063930933348E-4 28-29 0.0 0.0 0.0 0.006770529778305509 1.9767970155636525E-4 30-31 0.0 0.0 0.0 0.01927377090174561 1.9767970155636525E-4 32-33 0.0 0.0 0.0 0.04307770163082459 1.9767970155636525E-4 34-35 0.0 0.0 0.0 0.07933545355795459 1.9767970155636525E-4 36-37 0.0 0.0 0.0 0.13712381964626535 1.9767970155636525E-4 38-39 0.0 0.0 0.0 0.22319685636559938 2.1415301001939568E-4 40-41 0.0 0.0 0.0 0.31800074657033955 2.306263184824261E-4 42-43 0.0 0.0 0.0 0.41520973981068215 2.306263184824261E-4 44-45 0.0 0.0 0.0 0.530901785146545 2.306263184824261E-4 46-47 0.0 0.0 0.0 0.6715344194954358 2.306263184824261E-4 48-49 0.0 0.0 0.0 0.821029693797437 2.8004624387151743E-4 50-51 0.0 0.0 0.0 0.9808866791226842 3.294661692606087E-4 52-53 0.0 0.0 0.0 1.1416167497964722 3.294661692606087E-4 54-55 0.0 0.0 0.0 1.314718275125996 3.4593947772363915E-4 56-57 0.0 0.0 0.0 1.5148524996433528 3.624127861866696E-4 58-59 0.0 0.0 0.0 1.7307022604344406 3.624127861866696E-4 60-61 0.0 0.0 0.0 1.96732486319741 3.624127861866696E-4 62-63 0.0 0.0 0.0 2.2000268185461778 4.2830602003879136E-4 64-65 0.0 0.0 0.0 2.450075167706517 4.2830602003879136E-4 66-67 0.0 0.0 0.0 2.7320158420512826 4.2830602003879136E-4 68-69 0.0 0.0 0.0 3.042751859589426 4.2830602003879136E-4 70-71 0.0 0.0 0.0 3.374590185268711 4.2830602003879136E-4 72-73 0.0 0.0 0.0 3.7190141186137513 4.941992538909131E-4 74-75 0.0 0.0 0.0 4.069829695642447 5.930391046690957E-4 76-77 0.0 0.0 0.0 4.449440615864521 6.259857215951566E-4 78-79 0.0 0.0 0.0 4.868834576024812 6.259857215951566E-4 80-81 0.0 0.0 0.0 5.31641436696535 6.42459030058187E-4 82-83 0.0 0.0 0.0 5.78119229194129 6.589323385212174E-4 84-85 0.0 0.0 0.0 6.2587370309760795 6.589323385212174E-4 86-87 0.0 0.0 0.0 6.741652068569817 6.754056469842479E-4 88-89 0.0 0.0 0.0 7.257579616323467 6.918789554472783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7620 0.0 55.929626 1 GTATCAA 13540 0.0 42.787556 1 TATCAAC 17945 0.0 32.257893 2 ATCAACG 18150 0.0 31.747845 3 TCAACGC 18605 0.0 30.945364 4 CAACGCA 18880 0.0 30.494623 5 AACGCAG 19430 0.0 29.651022 6 TGGTATC 3085 0.0 27.136032 2 ACGCAGA 22050 0.0 26.063242 7 GTGGTAT 3295 0.0 25.983995 1 CGCAGAG 22365 0.0 25.674917 8 GTACATG 15080 0.0 24.508038 1 TACATGG 15240 0.0 24.157104 2 GCAGAGT 23880 0.0 23.568703 9 GAGTACT 13585 0.0 23.127136 12-13 ACATGGG 15700 0.0 22.287762 3 TAAGGTG 1635 0.0 22.099222 5 AGTACTT 14035 0.0 21.793406 12-13 GTACTTT 14355 0.0 21.754257 14-15 AGAGTAC 22130 0.0 21.204458 10-11 >>END_MODULE