FastQCFastQC Report
Thu 26 May 2016
SRR938625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032338
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147260.485631878768132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112700.37166041516480025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82320.2714736945551584No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG34350.11327892866824213No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG31840.10500148730121775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70150.055.1603471
GTATCAA130850.043.7753261
TATCAAC171600.032.9844172
ATCAACG173150.032.5793573
TCAACGC176350.031.9881844
CAACGCA178750.031.478935
AACGCAG183800.030.6865376
GTGGTAT30350.028.890681
TGGTATC29550.028.6270682
ACGCAGA211850.026.4163917
CGCAGAG214850.025.892758
GTACATG153750.025.260451
TACATGG156350.024.3471952
GCAGAGT225900.024.057599
GAGTACT124100.023.82725712-13
AGTACTT129350.022.60310712-13
ACATGGG163550.022.5489063
GGACCGA14900.022.3230956
GTACTTT132150.022.3050214-15
AGAGTAC212500.021.28080710-11
Produced by FastQC (version 0.11.5)