##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032338 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17559816880572 31.0 31.0 34.0 28.0 34.0 2 31.320120975959803 31.0 31.0 34.0 28.0 34.0 3 31.372025480009153 31.0 31.0 34.0 28.0 34.0 4 35.04362178622568 37.0 35.0 37.0 32.0 37.0 5 35.047528013038125 37.0 35.0 37.0 32.0 37.0 6 35.06857711772236 37.0 35.0 37.0 32.0 37.0 7 34.98843796436941 37.0 35.0 37.0 32.0 37.0 8 34.952663588293916 37.0 35.0 37.0 32.0 37.0 9 36.443331185375776 39.0 37.0 39.0 32.0 39.0 10-11 36.29399839331895 39.0 35.0 39.0 32.0 39.0 12-13 36.12600013586876 39.0 35.0 39.0 31.0 39.0 14-15 37.09554607698746 39.0 36.0 41.0 31.0 41.0 16-17 36.93586714277894 39.0 36.0 41.0 31.0 41.0 18-19 36.83226589516076 39.0 36.0 41.0 30.5 41.0 20-21 36.821725348559426 39.0 36.0 40.5 30.5 41.0 22-23 36.91879797041095 39.0 36.0 41.0 31.0 41.0 24-25 36.83025276206017 39.0 36.0 41.0 30.0 41.0 26-27 36.622834426769046 39.0 36.0 41.0 30.0 41.0 28-29 36.424524739656334 39.0 35.5 40.5 30.0 41.0 30-31 36.13466325323892 38.5 35.0 40.0 29.0 41.0 32-33 35.80922228986347 38.0 35.0 40.0 28.0 41.0 34-35 35.520116161193116 38.0 34.5 40.0 27.0 41.0 36-37 35.217591838376855 38.0 34.0 40.0 26.0 41.0 38-39 34.87748908597921 38.0 34.0 40.0 25.0 41.0 40-41 34.72760886154512 38.0 33.5 40.0 24.5 41.0 42-43 34.45522151554345 38.0 33.0 40.0 24.0 41.0 44-45 34.070259647836096 38.0 33.0 40.0 23.5 41.0 46-47 33.59159846296818 38.0 33.0 40.0 20.5 41.0 48-49 33.13316655333277 37.0 32.0 40.0 17.5 41.0 50-51 32.318636477859656 36.5 30.5 39.5 15.0 40.5 52-53 32.306786710452464 36.5 31.0 39.0 13.5 40.0 54-55 32.36098185624426 37.0 31.0 40.0 10.5 41.0 56-57 32.01074204128959 36.0 31.0 39.0 8.0 41.0 58-59 31.55865342188107 36.0 30.0 39.0 6.5 41.0 60-61 31.057232571039243 35.0 29.5 39.0 2.0 40.0 62-63 30.452320123943966 35.0 28.5 38.5 2.0 40.0 64-65 29.943010310855847 35.0 28.0 38.0 2.0 40.0 66-67 29.365555884601257 34.0 27.5 38.0 2.0 40.0 68-69 28.72678326096893 34.0 26.0 37.0 2.0 39.5 70-71 28.082503665488478 34.0 26.0 36.5 2.0 39.0 72-73 27.43150169934882 33.0 24.5 36.0 2.0 39.0 74-75 26.714471638715736 32.5 22.5 35.0 2.0 38.0 76-77 25.967093378112864 32.0 20.5 35.0 2.0 37.0 78-79 25.154350207661548 31.0 17.5 35.0 2.0 36.5 80-81 24.377000189292882 31.0 10.5 35.0 2.0 36.0 82-83 23.62568338358059 30.5 4.5 34.0 2.0 36.0 84-85 22.881570590085936 30.0 2.0 34.0 2.0 35.0 86-87 22.143671154073196 29.0 2.0 34.0 2.0 35.0 88-89 21.364853456309948 29.0 2.0 34.0 2.0 35.0 90-91 20.53937951508044 28.0 2.0 34.0 2.0 35.0 92-93 19.66338284188636 26.5 2.0 34.0 2.0 35.0 94-95 18.755156747038093 25.0 2.0 33.0 2.0 35.0 96-97 17.683074907876367 22.0 2.0 33.0 2.0 35.0 98-99 16.443346025410097 10.0 2.0 32.0 2.0 34.5 100-101 13.722610902874283 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6124787658004784 1101 2 0.5915611187943881 1101 3 0.6374926443923492 1101 4 0.49509634463094443 1101 5 0.5494930032329037 1101 6 0.5608338205217294 1101 7 0.5441744086552731 1101 8 0.465083071558027 1101 9 0.6398665155329581 1101 10-11 0.7458200029855675 1101 12-13 0.736715357090624 1101 14-15 0.8461873063889058 1101 16-17 0.9952780311432647 1101 18-19 1.055286330172983 1101 20-21 1.0388352256312317 1101 22-23 1.020091249098634 1101 24-25 1.105772666261288 1101 26-27 1.082727187547178 1101 28-29 1.2700528542270888 1101 30-31 1.167118747746855 1101 32-33 1.264577963341111 1101 34-35 1.3966125771662306 1101 36-37 1.3872068887881142 1101 38-39 1.6249067167645777 1101 40-41 1.6504191538545 1101 42-43 1.8531391428459116 1101 44-45 1.844033405446936 1101 46-47 1.9138043845017094 1101 48-49 1.979301519699142 1101 50-51 1.9305481794567427 1101 52-53 2.1954444994301454 1101 54-55 2.351676034387914 1101 56-57 2.424856306878283 1101 58-59 2.5980023446462646 1101 60-61 2.628431071896678 1101 62-63 2.7500157274409247 1101 64-65 2.735719998590316 1101 66-67 2.8051259136459628 1101 68-69 2.942880405789193 1101 70-71 3.0586746105385423 1101 72-73 3.3810823069577793 1101 74-75 3.7092621276372384 1101 76-77 3.9127724861357542 1101 78-79 4.267788638371226 1101 80-81 4.554427843592599 1101 82-83 4.858467256014151 1101 84-85 4.945556359716587 1101 86-87 5.107753276285862 1101 88-89 5.470078685858308 1101 90-91 5.732912232742688 1101 92-93 5.870095235749744 1101 94-95 6.0466524883478705 1101 96-97 6.101119216651121 1101 98-99 6.348264567855299 1101 100-101 6.052991340041011 1102 1 0.5546481769626652 1102 2 0.5759339461548123 1102 3 0.5372989395918566 1102 4 0.4824869468505 1102 5 0.4765391220467734 1102 6 0.5139908508220046 1102 7 0.5538568952356755 1102 8 0.5597616275968065 1102 9 0.6660035847393999 1102 10-11 0.6888829067953708 1102 12-13 0.6845630585842244 1102 14-15 0.8819930949546801 1102 16-17 0.9826982328881257 1102 18-19 1.0314924419931089 1102 20-21 1.0008535277749289 1102 22-23 0.7632843908197913 1102 24-25 0.6975408317653091 1102 26-27 0.8111343338362857 1102 28-29 0.8019232826492413 1102 30-31 0.912211765976032 1102 32-33 0.8405509961084761 1102 34-35 0.9891186657197508 1102 36-37 0.9975256963264982 1102 38-39 1.1557817751031791 1102 40-41 1.342354178077926 1102 42-43 1.6402607335657038 1102 44-45 1.664445513442523 1102 46-47 1.6505138534586834 1102 48-49 1.4840112860280925 1102 50-51 1.5367099745922346 1102 52-53 1.7162178593066812 1102 54-55 1.697292482362279 1102 56-57 1.9232510489012071 1102 58-59 1.9934458074878485 1102 60-61 1.9198879605322503 1102 62-63 2.247005524553387 1102 64-65 2.3051907728450622 1102 66-67 2.4056975286648985 1102 68-69 2.594705319778477 1102 70-71 2.57533501311962 1102 72-73 2.6276745755617696 1102 74-75 2.753294519154977 1102 76-77 3.0243812822886404 1102 78-79 3.3969313672822494 1102 80-81 3.915462202446232 1102 82-83 4.1848564030384345 1102 84-85 4.1535955907897595 1102 86-87 4.225412766100185 1102 88-89 4.729449689061941 1102 90-91 5.037718507291363 1102 92-93 5.007975331514537 1102 94-95 5.193647172823816 1102 96-97 5.350724441586863 1102 98-99 5.635773017487235 1102 100-101 5.7989454979482815 1103 1 0.54564527342189 1103 2 0.513729082367135 1103 3 0.4030744162350537 1103 4 0.4571623327045131 1103 5 0.5376351730029185 1103 6 0.5305327537403954 1103 7 0.5270737300621775 1103 8 0.45533087290461083 1103 9 0.767799526063321 1103 10-11 0.595664651603542 1103 12-13 0.675444705751211 1103 14-15 0.7150776859180183 1103 16-17 0.980105530853649 1103 18-19 1.0103818162329645 1103 20-21 0.9567240618322828 1103 22-23 0.8246723661068529 1103 24-25 0.960644237216087 1103 26-27 0.9848177122787192 1103 28-29 1.045170837336876 1103 30-31 1.0480896944981097 1103 32-33 1.0836415254836282 1103 34-35 1.0803744613812825 1103 36-37 1.043610841160934 1103 38-39 1.232025415578022 1103 40-41 1.2278730896355654 1103 42-43 1.2805592815098166 1103 44-45 1.2046753984999867 1103 46-47 1.4428653646131906 1103 48-49 1.3611307708708722 1103 50-51 1.5369915495275706 1103 52-53 1.752879165700179 1103 54-55 1.8066072595861868 1103 56-57 1.8670668567821735 1103 58-59 2.0741093536333075 1103 60-61 2.21017530257906 1103 62-63 2.303540347057691 1103 64-65 2.2932652356757437 1103 66-67 2.3549192898037 1103 68-69 2.5336806439643738 1103 70-71 2.756793642659794 1103 72-73 2.8133725316632017 1103 74-75 3.0776060345348917 1103 76-77 3.235098010052429 1103 78-79 3.4166762296995294 1103 80-81 3.792671441530114 1103 82-83 4.077221796658069 1103 84-85 4.438483938655072 1103 86-87 4.624269193574474 1103 88-89 4.889506125386749 1103 90-91 5.180787481373624 1103 92-93 5.0892635885906685 1103 94-95 5.386178585023824 1103 96-97 5.481682303130508 1103 98-99 5.6888275880658306 1103 100-101 5.599367574850717 1104 1 0.541921494116405 1104 2 0.4932593132855203 1104 3 0.41913734590189833 1104 4 0.5112562865208403 1104 5 0.4041905669743642 1104 6 0.3975012889069731 1104 7 0.4003881139860255 1104 8 0.5114795155980687 1104 9 0.5478486623227994 1104 10-11 0.6815787402691527 1104 12-13 0.6582304892872557 1104 14-15 0.7815493499405832 1104 16-17 0.8942768247016986 1104 18-19 1.0400536168675458 1104 20-21 0.9922073819451 1104 22-23 0.8813966521988732 1104 24-25 0.9449558888995 1104 26-27 0.9479847975951259 1104 28-29 0.9061873850188746 1104 30-31 0.9911285407875567 1104 32-33 0.9940229374349769 1104 34-35 1.048513012412485 1104 36-37 1.3127723297912866 1104 38-39 1.2571444002422183 1104 40-41 1.4191877600039717 1104 42-43 1.5387697741944564 1104 44-45 1.5238958286075217 1104 46-47 1.6263854377667357 1104 48-49 1.9186675679799805 1104 50-51 1.6951061934549472 1104 52-53 1.8690820125184402 1104 54-55 2.0728244286325506 1104 56-57 2.194698841622639 1104 58-59 2.333931096978983 1104 60-61 2.4011264086957524 1104 62-63 2.5210343978244794 1104 64-65 2.6116401034705596 1104 66-67 2.753873216297624 1104 68-69 3.091534182656421 1104 70-71 3.272002747190026 1104 72-73 3.380899082870677 1104 74-75 3.573513182428421 1104 76-77 3.723593404905241 1104 78-79 3.8595525737855496 1104 80-81 4.096523019178626 1104 82-83 4.342125176703899 1104 84-85 4.652160564778814 1104 86-87 4.814322406160411 1104 88-89 4.918861184438395 1104 90-91 5.194476226305756 1104 92-93 5.26570242165959 1104 94-95 5.428024279541717 1104 96-97 5.7789318844807624 1104 98-99 5.946947189687545 1104 100-101 5.829872818989621 1105 1 0.4971843763954169 1105 2 0.4736077283113538 1105 3 0.38815354475680763 1105 4 0.4470264303554998 1105 5 0.36158042174324834 1105 6 0.46991959640592285 1105 7 0.33896163723697015 1105 8 0.39585407920650084 1105 9 0.3935624243672038 1105 10-11 0.4054550319881898 1105 12-13 0.4810258390987343 1105 14-15 0.6921108986069697 1105 16-17 0.7859984767056076 1105 18-19 0.8273873516811179 1105 20-21 0.8508186967056091 1105 22-23 0.6675791860628593 1105 24-25 0.8402934814145127 1105 26-27 0.8151921534113526 1105 28-29 0.9919684386426724 1105 30-31 0.9446096363049321 1105 32-33 0.981996858675025 1105 34-35 1.009591248692331 1105 36-37 1.1519424003121586 1105 38-39 1.3318808197367034 1105 40-41 1.2619746438190802 1105 42-43 1.4915849361830098 1105 44-45 1.4286472599718536 1105 46-47 1.4999248351972554 1105 48-49 1.5253277760514834 1105 50-51 1.541641680817044 1105 52-53 1.6601883524151049 1105 54-55 1.8941334287721858 1105 56-57 1.9231341160256648 1105 58-59 1.9693128523204173 1105 60-61 2.210913362333514 1105 62-63 2.4357364296636064 1105 64-65 2.725418294365692 1105 66-67 2.6893592692231927 1105 68-69 2.761265627287063 1105 70-71 2.9525449200709097 1105 72-73 3.0660901350980154 1105 74-75 3.1932214983060945 1105 76-77 3.4161847852990412 1105 78-79 3.499251987275283 1105 80-81 3.856712762231272 1105 82-83 4.213244346512585 1105 84-85 4.313842766985207 1105 86-87 4.411329196413167 1105 88-89 4.5473174056365515 1105 90-91 4.771697459373765 1105 92-93 4.945219074361727 1105 94-95 5.1983605628820335 1105 96-97 5.533937732127736 1105 98-99 5.518812152681544 1105 100-101 5.185849637869666 1106 1 0.32186967149887735 1106 2 0.31367124205993946 1106 3 0.29499315238863844 1106 4 0.3368368649388813 1106 5 0.32733868340970673 1106 6 0.17742838947498996 1106 7 0.304895654216395 1106 8 0.3220688804131342 1106 9 0.3904493172848049 1106 10-11 0.2917435719356689 1106 12-13 0.0826371769604819 1106 14-15 0.6097745196967566 1106 16-17 0.3654800780836496 1106 18-19 0.5501008355184211 1106 20-21 0.44094818498930266 1106 22-23 0.4254797427097401 1106 24-25 0.5746689719807918 1106 26-27 0.6897087997835953 1106 28-29 0.5266089410674013 1106 30-31 0.7613357719791338 1106 32-33 0.9169084718902951 1106 34-35 0.7719749977430226 1106 36-37 0.8253459120242397 1106 38-39 0.8223301572335728 1106 40-41 0.9822315308392859 1106 42-43 0.7704405749904666 1106 44-45 0.9245926368752464 1106 46-47 1.1560188351208822 1106 48-49 1.3269339165623961 1106 50-51 0.7467986726328064 1106 52-53 1.2774661497566058 1106 54-55 1.005931998546096 1106 56-57 1.1298204548420188 1106 58-59 0.40119149311771096 1106 60-61 1.709947132385743 1106 62-63 1.9764882211586183 1106 64-65 2.1015884938166813 1106 66-67 2.019572424248498 1106 68-69 1.621406560349424 1106 70-71 2.064184917610124 1106 72-73 2.489564606559256 1106 74-75 2.763949116816299 1106 76-77 2.819577382574355 1106 78-79 3.2378471433443075 1106 80-81 3.643403672595003 1106 82-83 3.9876941167684663 1106 84-85 4.001034426191733 1106 86-87 4.117062139556271 1106 88-89 4.345092992512921 1106 90-91 4.735753959880771 1106 92-93 4.877257719316901 1106 94-95 4.932166148018883 1106 96-97 5.435113659271487 1106 98-99 5.559375089418115 1106 100-101 4.845265699592979 1107 1 0.3786061748294678 1107 2 0.2072958278335726 1107 3 0.2406741391482754 1107 4 0.16627336312740937 1107 5 0.2678396275076409 1107 6 0.25128284515842836 1107 7 0.26624680886710195 1107 8 0.21026648113068802 1107 9 0.23984298954503913 1107 10-11 0.4147195500651435 1107 12-13 0.3059621382692441 1107 14-15 0.49564382107249827 1107 16-17 0.6611212882327564 1107 18-19 0.5649937917380825 1107 20-21 0.5603480143876141 1107 22-23 0.5732679404438841 1107 24-25 0.572537015448809 1107 26-27 0.6515972029643748 1107 28-29 0.659336834462998 1107 30-31 0.7572596316128042 1107 32-33 0.8535143256566755 1107 34-35 0.892478370210462 1107 36-37 0.9448359072136796 1107 38-39 0.9717383114438647 1107 40-41 1.0386541730551642 1107 42-43 0.9204514082280895 1107 44-45 1.1399565797755145 1107 46-47 0.9524227339369062 1107 48-49 1.1939242871432825 1107 50-51 1.287387801645636 1107 52-53 1.3612119518128765 1107 54-55 1.5425834432566106 1107 56-57 1.5331893608122868 1107 58-59 1.6182208089569237 1107 60-61 1.556890111785755 1107 62-63 1.7196237409071813 1107 64-65 1.7803471269217575 1107 66-67 2.1080292734257107 1107 68-69 2.3115853554696564 1107 70-71 2.2698276334304346 1107 72-73 2.6663712041703214 1107 74-75 2.5379357574996178 1107 76-77 2.8543211305748777 1107 78-79 3.0212356076448614 1107 80-81 3.340578193846472 1107 82-83 3.607793564966137 1107 84-85 3.8133492258107076 1107 86-87 4.03489518822845 1107 88-89 4.315384929535842 1107 90-91 4.605872010866886 1107 92-93 4.7687810880627595 1107 94-95 4.711408063606253 1107 96-97 4.93032065724535 1107 98-99 5.113033023704958 1107 100-101 4.916248397922054 1108 1 0.3951856730706602 1108 2 0.38045883352439347 1108 3 0.32150595677536487 1108 4 0.30229012142262235 1108 5 0.2879905560706959 1108 6 0.412106115035499 1108 7 0.3532402603299474 1108 8 0.22237615307646763 1108 9 0.44337197519079297 1108 10-11 0.4511213001973573 1108 12-13 0.5204455262758287 1108 14-15 0.5357098308759518 1108 16-17 0.6455822236918536 1108 18-19 0.6571804914586608 1108 20-21 0.6266164558887013 1108 22-23 0.5521312759386632 1108 24-25 0.5458124554202186 1108 26-27 0.581799726805194 1108 28-29 0.7660250567515519 1108 30-31 0.8356706813441406 1108 32-33 0.962429472083457 1108 34-35 0.918761427187782 1108 36-37 1.0186388668721236 1108 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0.0884356827801156 2311 42-43 0.32192043401850867 2311 44-45 0.3740693378939284 2311 46-47 0.3725114152838387 2311 48-49 0.3756849189086253 2311 50-51 0.5175615942369589 2311 52-53 0.6933268805536343 2311 54-55 0.7087762859150502 2311 56-57 0.8989999168914551 2311 58-59 0.9732089562165136 2311 60-61 0.9184999424200981 2311 62-63 1.1543078582350361 2311 64-65 1.199325220772618 2311 66-67 1.0165021263660456 2311 68-69 1.1199561034775378 2311 70-71 1.3130535780795647 2311 72-73 1.4357762822841664 2311 74-75 1.3551370827216758 2311 76-77 1.5756003697146248 2311 78-79 1.9291762296995252 2311 80-81 1.8498404678589786 2311 82-83 2.0358525716208113 2311 84-85 2.445866576509019 2311 86-87 2.6140456466296165 2311 88-89 2.556678877498026 2311 90-91 2.774338152014458 2311 92-93 3.1407060873487396 2311 94-95 2.924702554224023 2311 96-97 2.8714593771493817 2311 98-99 3.211182282551672 2311 100-101 3.2284578629778906 2312 1 -0.30367370094452184 2312 2 -0.3074707184556793 2312 3 -0.2891813217921566 2312 4 -0.20202491641038733 2312 5 -0.3317231855901426 2312 6 -0.1826569335224164 2312 7 -0.2989214911356868 2312 8 -0.15912319064838698 2312 9 -0.3412062719909912 2312 10-11 -0.3567093297485684 2312 12-13 -0.30058720651091875 2312 14-15 -0.25869782157288057 2312 16-17 -0.1600224074529919 2312 18-19 -0.191630139232565 2312 20-21 -0.23222867467853092 2312 22-23 -0.23977388183651271 2312 24-25 -0.3249142686649549 2312 26-27 -0.13278913395360092 2312 28-29 -0.14212108212850438 2312 30-31 -0.1697270149529757 2312 32-33 -0.004449358776057011 2312 34-35 0.044114212458147506 2312 36-37 0.009807423080609112 2312 38-39 0.06367013407910349 2312 40-41 0.16508242147344987 2312 42-43 0.11300570429722967 2312 44-45 0.1476617348806073 2312 46-47 0.218033742656651 2312 48-49 0.47533787727435595 2312 50-51 0.5949560787458594 2312 52-53 0.5096495886132928 2312 54-55 0.6663090598353207 2312 56-57 0.7669715083590702 2312 58-59 0.9205857021482622 2312 60-61 1.071897125694889 2312 62-63 1.1766446588570005 2312 64-65 1.1981538491382544 2312 66-67 1.299102592841475 2312 68-69 1.4950931864869297 2312 70-71 1.447236129038881 2312 72-73 1.5318677186174163 2312 74-75 1.5380252090453723 2312 76-77 1.8422808876632075 2312 78-79 1.889391996926502 2312 80-81 2.100532585139124 2312 82-83 2.34661251550078 2312 84-85 2.505898657865192 2312 86-87 2.806697809663671 2312 88-89 2.839953901402655 2312 90-91 2.9025728284753214 2312 92-93 3.000624856282549 2312 94-95 3.2230057275080277 2312 96-97 3.3746872620139534 2312 98-99 3.5251953116929826 2312 100-101 3.2972130348678306 2313 1 -0.37357355419857896 2313 2 -0.4543782201663973 2313 3 -0.37301847993980886 2313 4 -0.31647154707690817 2313 5 -0.25585091740940413 2313 6 -0.2503912402983559 2313 7 -0.17946501807492865 2313 8 -0.08533604214755286 2313 9 -0.17704647493022208 2313 10-11 -0.25180492117340236 2313 12-13 -0.3670074800411456 2313 14-15 -0.2683612112503866 2313 16-17 -0.2550969033241941 2313 18-19 -0.2035898678206891 2313 20-21 -0.23211721983686573 2313 22-23 -0.33099703280024784 2313 24-25 -0.30986619497467416 2313 26-27 -0.15433094643961454 2313 28-29 -0.009529858142506953 2313 30-31 -0.06252257574530518 2313 32-33 -0.07140690968775942 2313 34-35 -0.1763226325084375 2313 36-37 -0.0981986471649492 2313 38-39 0.017879861678373743 2313 40-41 0.10304074984419742 2313 42-43 0.23163975840996187 2313 44-45 0.30293670003997875 2313 46-47 0.14908121160772936 2313 48-49 0.3458861111590039 2313 50-51 0.5183688470933845 2313 52-53 0.4424376604840816 2313 54-55 0.4666451383740693 2313 56-57 0.7198022020380108 2313 58-59 0.8572626074832819 2313 60-61 0.8990041649734906 2313 62-63 1.1233615095018017 2313 64-65 1.0297375406931302 2313 66-67 1.144490203862322 2313 68-69 1.5329789549430117 2313 70-71 1.5505250137477233 2313 72-73 1.6432377825325517 2313 74-75 1.6293695715443306 2313 76-77 1.913975928581987 2313 78-79 1.8107832838475666 2313 80-81 2.0110426536513266 2313 82-83 2.193165040572623 2313 84-85 2.442672339052489 2313 86-87 2.461986530881976 2313 88-89 2.4910926877315056 2313 90-91 2.6715537506234988 2313 92-93 2.7286792617153566 2313 94-95 2.9619703137868676 2313 96-97 2.9775746280187327 2313 98-99 2.8913611201075575 2313 100-101 2.7808224928835052 2314 1 -0.5739896709000014 2314 2 -0.48853937407321624 2314 3 -0.5477770959891366 2314 4 -0.40203657055447195 2314 5 -0.3794367039044815 2314 6 -0.38236236379160715 2314 7 -0.4400297466356804 2314 8 -0.4535881379138118 2314 9 -0.5046897924865945 2314 10-11 -0.3710508866021627 2314 12-13 -0.35395376280039414 2314 14-15 -0.5047448145746003 2314 16-17 -0.5373677715287144 2314 18-19 -0.4934600214089997 2314 20-21 -0.3773268751075065 2314 22-23 -0.34294439692114764 2314 24-25 -0.30976496204762327 2314 26-27 -0.3560093071040953 2314 28-29 -0.3843673371324101 2314 30-31 -0.3423500472246701 2314 32-33 -0.27309938266792244 2314 34-35 -0.39663864352041145 2314 36-37 -0.19229030556302007 2314 38-39 -0.16885757628532616 2314 40-41 -0.10545573802423291 2314 42-43 0.079288441267785 2314 44-45 0.12361412614583855 2314 46-47 0.2528289707860907 2314 48-49 0.19435326463501923 2314 50-51 0.16693727718784146 2314 52-53 0.3698072515458719 2314 54-55 0.20384651325440473 2314 56-57 0.30976655424120736 2314 58-59 0.6037918298073102 2314 60-61 0.5022459779735158 2314 62-63 0.7398169065013391 2314 64-65 0.7447248636392771 2314 66-67 0.7992206040185614 2314 68-69 1.117457635952686 2314 70-71 1.1525304043901663 2314 72-73 1.0765358569223693 2314 74-75 1.22921053492178 2314 76-77 1.3778297879071069 2314 78-79 1.6853840866329755 2314 80-81 1.8946527063387641 2314 82-83 1.9823036256971953 2314 84-85 2.281742219482286 2314 86-87 2.3662773302201394 2314 88-89 2.5307140844487996 2314 90-91 2.62128825995401 2314 92-93 2.72409752147896 2314 94-95 2.980648291277941 2314 96-97 3.20828328909203 2314 98-99 3.158784958386537 2314 100-101 3.0894522394603925 2315 1 -0.5691663299992129 2315 2 -0.6215996265592523 2315 3 -0.4866876828296647 2315 4 -0.30822922175227774 2315 5 -0.29678935635566717 2315 6 -0.26518526944564513 2315 7 -0.38916579901638215 2315 8 -0.43897792626972176 2315 9 -0.4302753045217713 2315 10-11 -0.4181450136469991 2315 12-13 -0.37714357201723914 2315 14-15 -0.5091281441950315 2315 16-17 -0.2959960660868788 2315 18-19 -0.17442664691179033 2315 20-21 -0.28125238009460674 2315 22-23 -0.39066628369432266 2315 24-25 -0.5199335536295138 2315 26-27 -0.5969135300706156 2315 28-29 -0.4895771204834105 2315 30-31 -0.3607315817742034 2315 32-33 -0.2947094694039265 2315 34-35 -0.3883278344207426 2315 36-37 -0.1449294503847227 2315 38-39 -0.09726176169357359 2315 40-41 -0.18632806006561253 2315 42-43 -0.12638272938713868 2315 44-45 -0.20830654391094328 2315 46-47 -0.13973380411365355 2315 48-49 -0.17638283032603397 2315 50-51 0.091334831736404 2315 52-53 0.21316272065300979 2315 54-55 0.3299249615439592 2315 56-57 0.4686314444658848 2315 58-59 0.7449778900645079 2315 60-61 0.7267152580951475 2315 62-63 0.7320404550671924 2315 64-65 0.9183659945425227 2315 66-67 1.1424358412602338 2315 68-69 1.2951699550237485 2315 70-71 1.2287326232900888 2315 72-73 1.398993816336251 2315 74-75 1.4983062561543932 2315 76-77 1.6417856485646176 2315 78-79 2.0241371862637045 2315 80-81 2.072272696440546 2315 82-83 2.286791388782177 2315 84-85 2.4043123815580465 2315 86-87 2.6619455090902413 2315 88-89 2.716709269263408 2315 90-91 2.8342114826857348 2315 92-93 2.9692685790298263 2315 94-95 3.160540878905689 2315 96-97 3.132044585011311 2315 98-99 3.198258017943658 2315 100-101 3.0125844879390655 2316 1 -1.056383772627278 2316 2 -0.9937651345160532 2316 3 -0.8971289337074921 2316 4 -0.7294548324894023 2316 5 -0.7154222893503714 2316 6 -0.6547076282213027 2316 7 -0.730575727754939 2316 8 -0.839317987783744 2316 9 -0.9358245186561049 2316 10-11 -0.942293011214403 2316 12-13 -0.8538144468779194 2316 14-15 -1.3258692273277077 2316 16-17 -1.1079944831964426 2316 18-19 -1.0897144017842706 2316 20-21 -0.8277090045493622 2316 22-23 -0.9239273293072898 2316 24-25 -1.0601088098968745 2316 26-27 -0.9358179475987427 2316 28-29 -0.9134488292745928 2316 30-31 -0.9239766595540857 2316 32-33 -0.8576588980261874 2316 34-35 -0.8106976795511471 2316 36-37 -0.8574398420246254 2316 38-39 -0.8293991881342109 2316 40-41 -0.8856996095369922 2316 42-43 -0.8341753725318455 2316 44-45 -0.8031299274871486 2316 46-47 -0.6764126740947987 2316 48-49 -0.5288498224443003 2316 50-51 -0.5238908085790825 2316 52-53 -0.4964680383321962 2316 54-55 -0.5664578738645645 2316 56-57 -0.28033127380336964 2316 58-59 -0.029178555261903938 2316 60-61 -0.2757623777513203 2316 62-63 -0.07131350693178717 2316 64-65 0.06919972337439262 2316 66-67 0.17835091730574604 2316 68-69 0.530848359369795 2316 70-71 0.3647125924658532 2316 72-73 0.7148947395844409 2316 74-75 0.9968481326145451 2316 76-77 1.0343239693473159 2316 78-79 1.1200929728889975 2316 80-81 1.5398618942440407 2316 82-83 1.6893052805280675 2316 84-85 1.6870756368089879 2316 86-87 1.7730891115821699 2316 88-89 1.8932032117496327 2316 90-91 2.05692309232667 2316 92-93 2.233179304367411 2316 94-95 2.1355320614171696 2316 96-97 1.960743123705857 2316 98-99 2.13603688922446 2316 100-101 2.231515714217558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9341.0 3 3042.0 4 3343.0 5 3870.0 6 4493.0 7 5797.0 8 7524.0 9 9733.0 10 12312.0 11 15972.0 12 20443.0 13 24672.0 14 29128.0 15 35751.0 16 42482.0 17 50440.0 18 46755.0 19 41944.0 20 44003.0 21 48294.0 22 52903.0 23 59769.0 24 66548.0 25 74041.0 26 83732.0 27 93592.0 28 104645.0 29 116595.0 30 130317.0 31 148065.0 32 167931.0 33 194184.0 34 228222.0 35 267466.0 36 306035.0 37 306316.0 38 161492.0 39 11146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55642029266911 18.494473132414267 15.071741609140958 22.877364965775662 2 15.689898216470702 20.166882365186225 39.696731070516755 24.446488347826318 3 19.072856771455378 24.4344088462244 29.500314748854166 26.992419633466053 4 13.142801148432454 16.690113939227572 36.15193304725901 34.01515186508096 5 14.172949809979531 37.266186718003155 32.98641164732914 15.574451824688174 6 32.21811042458939 35.38727799180024 17.33344332346172 15.061168260148653 7 29.100239888527046 32.78641476172129 19.845596501691624 18.267748848060045 8 27.272325407858354 33.723537123406885 19.528311410005273 19.475826058729492 9 27.49707449151248 16.458958588260288 19.556648962405358 36.487317957821865 10-11 25.471772026020634 26.332985140185265 26.536919772241106 21.658323061552995 12-13 26.579991996825118 23.79384138208826 27.033840629934975 22.592325991151643 14-15 23.304564709281966 25.030517216724274 25.90253194349767 25.76238613049609 16-17 22.54790911171965 28.16615089069486 26.36722963322622 22.918710364359278 18-19 22.5744090987584 26.29130560777059 29.30591751471078 21.82836777876023 20-21 23.37474353683123 25.45609122913283 29.388048115278192 21.78111711875775 22-23 23.053240933938962 25.218558963993726 29.240452962442596 22.487747139624716 24-25 22.72660831738 25.53192086089285 29.34659951417389 22.39487130755327 26-27 22.1612033112538 26.029760108798055 29.278640446607838 22.530396133340304 28-29 22.250693048532696 26.23520663452729 29.37225951801497 22.141840798925035 30-31 22.325255632037006 25.802319802280543 29.107931657266263 22.76449290841619 32-33 22.280546879432904 25.891578816801196 28.97448890050104 22.853385403264863 34-35 23.022727377691975 26.028676597207422 28.621060382235868 22.327535642864728 36-37 23.04844498919704 25.53580579976779 28.796816463184417 22.61893274785075 38-39 22.442571511221164 25.993831238762592 28.60567590683462 22.957921343181624 40-41 22.75890765291431 25.755133921135243 28.69708455224001 22.78887387371044 42-43 22.752322235190643 25.767785221838334 28.422281913241758 23.05761062972926 44-45 22.472834681636353 26.131425162173986 28.450062217953796 22.94567793823586 46-47 22.758081467998604 26.476332681746346 27.78862656644332 22.97695928381173 48-49 22.825879022075984 26.192770059091814 27.90274191824775 23.078609000584454 50-51 23.112451266072117 26.60211628468018 27.397029866726736 22.88840258252097 52-53 23.187685983747045 26.56498428362103 27.292808787848504 22.954520944783418 54-55 22.64238340704389 26.61960404072385 27.58539376609842 23.152618786133843 56-57 22.678595603787837 26.679613353897206 27.64647595779734 22.995315084517618 58-59 22.763018097430653 26.172271471848614 27.61944589511844 23.445264535602288 60-61 22.69680211811359 26.40016991344704 27.856504927885727 23.046523040553645 62-63 22.48750911051675 26.615224539197484 27.76483159147948 23.132434758806284 64-65 22.283646413668404 26.343865354156073 27.698915191686297 23.67357304048923 66-67 22.369314296521857 26.46773509773468 27.610247189766202 23.552703415977263 68-69 22.485162118449765 26.27340618923362 27.773288779012855 23.468142913303765 70-71 22.47210504269586 26.491469073950086 27.40769020188594 23.628735681468118 72-73 22.577141022366018 26.50576858031362 27.30610869724036 23.61098170008 74-75 22.363305197177095 26.73298741005266 27.27154592745836 23.63216146531189 76-77 22.49307682958785 26.362664467039142 27.277678953215645 23.866579750157364 78-79 22.69481629865275 26.483727697954308 27.365322011215042 23.456133992177897 80-81 22.68092084108226 26.820454589677723 27.07872094338621 23.41990362585381 82-83 22.63810516087986 26.612814121978918 27.01835729290039 23.730723424240836 84-85 22.711821198370362 26.284529482544 27.129012688602156 23.87463663048348 86-87 22.774997613193573 26.360961971206955 26.904296399970384 23.959744015629088 88-89 22.667831463160976 26.277667077764843 27.04881425278151 24.005687206292674 90-91 22.615589977685342 26.239161210332266 27.111410494536297 24.03383831744609 92-93 22.703029765313946 25.748494797736665 27.23000730729544 24.318468129653947 94-95 22.492540727684567 25.87672735075312 27.304375419225863 24.32635650233645 96-97 22.665146974325754 25.69431754687221 27.2010525919979 24.43948288680414 98-99 22.629549573974366 25.71027222532532 26.869538214829987 24.79063998587033 100-101 22.950703583681296 25.225977397638346 27.18031048535125 24.64300853332911 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1325.0 1 896.0 2 571.5 3 680.0 4 603.0 5 639.5 6 1090.0 7 2421.0 8 4058.0 9 4602.0 10 3932.0 11 3337.0 12 3486.0 13 3827.0 14 4176.0 15 4479.5 16 4843.0 17 5265.0 18 5664.5 19 6127.5 20 6688.5 21 7676.0 22 8796.5 23 10041.0 24 11696.0 25 13891.5 26 16759.0 27 20347.0 28 23825.5 29 27217.5 30 31671.0 31 36703.5 32 42378.5 33 45744.5 34 47474.5 35 50190.5 36 54495.0 37 60195.5 38 64534.0 39 68271.0 40 73034.0 41 81062.5 42 89685.0 43 96001.0 44 99960.0 45 104302.0 46 107405.5 47 110502.0 48 114878.0 49 116758.0 50 117529.0 51 115279.5 52 112862.0 53 112207.5 54 111660.5 55 105756.0 56 94703.5 57 85992.5 58 79904.5 59 71643.5 60 60273.5 61 50626.0 62 42700.0 63 34571.5 64 28119.0 65 23219.5 66 18350.5 67 14406.0 68 11177.5 69 8512.0 70 6756.0 71 5322.5 72 4378.0 73 3567.5 74 2744.5 75 2181.0 76 1583.0 77 1034.5 78 659.5 79 431.0 80 243.0 81 126.5 82 82.0 83 60.5 84 37.0 85 24.5 86 19.5 87 13.5 88 6.0 89 4.0 90 5.0 91 5.0 92 2.0 93 1.5 94 2.0 95 4.0 96 3.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30329732371523227 2 0.036308617311130886 3 0.044520102970051496 4 0.04696046416989135 5 0.06183347634729374 6 0.05813995669348206 7 0.03066940426825769 8 0.03350549971672023 9 0.04092551687839548 10-11 0.0321369187735668 12-13 0.03432994606801748 14-15 0.028113620579236217 16-17 0.021765383674247397 18-19 0.026497705730693613 20-21 0.039606402716319884 22-23 0.03314274332214945 24-25 0.05355603498026935 26-27 0.046218462453723824 28-29 0.038419199970451845 30-31 0.0387324895839448 32-33 0.033967189673446696 34-35 0.03350549971672023 36-37 0.03273052014650082 38-39 0.020149468825704786 40-41 0.019489911744666986 42-43 0.020264891314886402 44-45 0.008409352783231948 46-47 0.016802216639437952 48-49 0.014708122907142938 50-51 0.006084414072573704 52-53 0.004979655961835389 54-55 0.006859393642793118 56-57 0.005259967721276454 58-59 0.007337572526545523 60-61 0.006793437934689339 62-63 0.004221165318641919 64-65 0.007766284629220094 66-67 0.0023908944187620244 68-69 0.007716817848142259 70-71 0.014114521534208918 72-73 0.014493766855805654 74-75 0.011723627115446892 76-77 0.010206645829059953 78-79 0.005309434502354288 80-81 0.0064966372482223285 82-83 0.011707138188420948 84-85 0.004237654245667865 86-87 0.0014345366512572148 88-89 0.020479247366223686 90-91 0.030422070362868515 92-93 0.014394833293649982 94-95 0.02336480959576406 96-97 0.019259066766303755 98-99 0.013834209774767854 100-101 0.10796949416588784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3032338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.82742104884855 #Duplication Level Percentage of deduplicated Percentage of total 1 69.49105076034543 29.761224900402212 2 14.651671223206261 12.549865850911049 3 5.921764060163512 7.608416482696851 4 3.108953826156202 5.3259389813688145 5 1.7917703984827682 3.8368452639342334 6 1.1871929898554714 3.050664842566899 7 0.7502122364304699 2.2490758727924236 8 0.5351320982857489 1.8334662152030061 9 0.3958498440845396 1.5257905150256668 >10 1.9418529902992507 14.66725564778779 >50 0.12250750300233325 3.63896190869549 >100 0.08904397315418396 7.525064509381769 >500 0.009028617951772847 2.647806080779775 >1k 0.0037359798420236066 2.638672611441307 >5k 7.783291337549181E-5 0.27440408465727006 >10k+ 1.5566582675098363E-4 0.8665462323554899 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14726 0.485631878768132 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11270 0.37166041516480025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8232 0.2714736945551584 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3435 0.11327892866824213 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3184 0.10500148730121775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.297785405188999E-5 0.0 6 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 7 0.0 0.0 0.0 3.297785405188999E-5 6.595570810377999E-5 8 0.0 0.0 0.0 3.297785405188999E-5 9.893356215566998E-5 9 0.0 0.0 0.0 3.297785405188999E-5 9.893356215566998E-5 10-11 0.0 0.0 0.0 4.946678107783499E-5 9.893356215566998E-5 12-13 0.0 0.0 0.0 6.595570810377999E-5 9.893356215566998E-5 14-15 0.0 0.0 0.0 9.893356215566998E-5 1.3191141620755997E-4 16-17 0.0 0.0 0.0 1.3191141620755997E-4 1.6488927025944998E-4 18-19 0.0 0.0 0.0 1.6488927025944995E-4 1.6488927025944998E-4 20-21 0.0 0.0 0.0 2.968006864670099E-4 1.9786712431133996E-4 22-23 0.0 0.0 0.0 5.606235188821299E-4 1.9786712431133996E-4 24-25 0.0 0.0 0.0 0.0010388024026345348 1.9786712431133996E-4 26-27 0.0 0.0 0.0 0.0021105826593209595 2.1435605133728497E-4 28-29 0.0 0.0 0.0 0.006018458364469924 2.3084497836322996E-4 30-31 0.0 0.0 0.0 0.01805537509340977 2.4733390538917495E-4 32-33 0.0 0.0 0.0 0.041024450440551155 3.4626746754484495E-4 34-35 0.0 0.0 0.0 0.07570066397611348 3.6275639457078994E-4 36-37 0.0 0.0 0.0 0.1322906615291567 3.6275639457078994E-4 38-39 0.0 0.0 0.0 0.2181485045532523 3.6275639457078994E-4 40-41 0.0 0.0 0.0 0.3118385879146718 3.6275639457078994E-4 42-43 0.0 0.0 0.0 0.40813392174619056 3.6275639457078994E-4 44-45 0.0 0.0 0.0 0.5261451724708789 3.6275639457078994E-4 46-47 0.0 0.0 0.0 0.6650643826644655 3.6275639457078994E-4 48-49 0.0 0.0 0.0 0.8099196065873924 3.6275639457078994E-4 50-51 3.297785405188999E-5 0.0 0.0 0.9695489091255658 3.957342486226799E-4 52-53 3.297785405188999E-5 0.0 0.0 1.1276117635962746 4.287121026745699E-4 54-55 3.297785405188999E-5 0.0 0.0 1.300729008441671 4.4520102970051494E-4 56-57 3.297785405188999E-5 0.0 0.0 1.5018939181582 4.6168995672645993E-4 58-59 3.297785405188999E-5 0.0 0.0 1.716827081941393 4.6168995672645993E-4 60-61 3.297785405188999E-5 0.0 0.0 1.9494033976423473 4.6168995672645993E-4 62-63 3.297785405188999E-5 0.0 0.0 2.183628606045896 4.781788837524049E-4 64-65 3.297785405188999E-5 0.0 0.0 2.430385398989163 4.946678107783499E-4 66-67 3.297785405188999E-5 0.0 0.0 2.7111258705329027 4.946678107783499E-4 68-69 3.297785405188999E-5 0.0 0.0 3.0192874277207884 5.111567378042949E-4 70-71 3.297785405188999E-5 1.6488927025944996E-5 0.0 3.351209528753061 5.441345918561849E-4 72-73 3.297785405188999E-5 3.297785405188999E-5 0.0 3.6869240830013013 5.936013729340198E-4 74-75 3.297785405188999E-5 3.297785405188999E-5 0.0 4.037181870886425 7.090238621156349E-4 76-77 3.297785405188999E-5 3.297785405188999E-5 0.0 4.409205042445796 7.255127891415799E-4 78-79 3.297785405188999E-5 4.946678107783499E-5 0.0 4.816465051059612 7.255127891415799E-4 80-81 3.297785405188999E-5 6.595570810377999E-5 0.0 5.246892002144879 7.255127891415799E-4 82-83 3.297785405188999E-5 6.595570810377999E-5 0.0 5.687970800088908 7.255127891415799E-4 84-85 3.297785405188999E-5 6.595570810377999E-5 0.0 6.1437742098671055 7.255127891415799E-4 86-87 6.595570810377999E-5 6.595570810377999E-5 0.0 6.60178713586678 7.420017161675249E-4 88-89 6.595570810377999E-5 6.595570810377999E-5 0.0 7.097971927931518 7.584906431934698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7015 0.0 55.160347 1 GTATCAA 13085 0.0 43.775326 1 TATCAAC 17160 0.0 32.984417 2 ATCAACG 17315 0.0 32.579357 3 TCAACGC 17635 0.0 31.988184 4 CAACGCA 17875 0.0 31.47893 5 AACGCAG 18380 0.0 30.686537 6 GTGGTAT 3035 0.0 28.89068 1 TGGTATC 2955 0.0 28.627068 2 ACGCAGA 21185 0.0 26.416391 7 CGCAGAG 21485 0.0 25.89275 8 GTACATG 15375 0.0 25.26045 1 TACATGG 15635 0.0 24.347195 2 GCAGAGT 22590 0.0 24.05759 9 GAGTACT 12410 0.0 23.827257 12-13 AGTACTT 12935 0.0 22.603107 12-13 ACATGGG 16355 0.0 22.548906 3 GGACCGA 1490 0.0 22.323095 6 GTACTTT 13215 0.0 22.30502 14-15 AGAGTAC 21250 0.0 21.280807 10-11 >>END_MODULE