FastQCFastQC Report
Thu 26 May 2016
SRR938625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032338
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143190.47220989216901277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115810.381916527774938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.28064153798158387No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG36690.12099574651638438No Hit
ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT33710.11116834600892117No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC31030.10233028112301465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69150.058.6746641
GTATCAA127400.042.1520081
TATCAAC170550.031.3194852
ATCAACG174450.030.5511533
TCAACGC178350.029.883094
CAACGCA181250.029.3787275
TGGTATC28650.028.7215612
GTGGTAT29700.028.507381
AACGCAG187450.028.4281776
GTACATG152200.025.564211
TACATGG153600.024.9901522
ACGCAGA217900.024.4337437
CGCAGAG220900.024.037418
ACATGGG158850.023.315423
GTAAGGT15600.022.2480134
GCAGAGT236550.022.0053989
GAGTACT137050.021.88446212-13
TAAGGTG17650.021.8189225
AGTACTT140950.021.34632512-13
GGACCGA16150.021.1739356
Produced by FastQC (version 0.11.5)