##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032338 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.809627422800492 31.0 31.0 34.0 30.0 34.0 2 32.034534408763136 31.0 31.0 34.0 30.0 34.0 3 32.09467513186195 33.0 31.0 34.0 30.0 34.0 4 35.77651534888261 37.0 35.0 37.0 35.0 37.0 5 35.64824073041989 37.0 35.0 37.0 33.0 37.0 6 35.69066772899327 37.0 35.0 37.0 33.0 37.0 7 35.62518591265222 37.0 35.0 37.0 33.0 37.0 8 35.61309293357139 37.0 35.0 37.0 33.0 37.0 9 37.16816166271702 39.0 37.0 39.0 33.0 39.0 10-11 37.10156387579485 39.0 37.0 39.0 33.0 39.0 12-13 36.993470220008454 39.0 37.0 39.0 33.0 39.0 14-15 38.01876275006282 40.0 37.0 41.0 33.0 41.0 16-17 37.957079322951465 40.0 37.0 41.0 33.0 41.0 18-19 37.98549353667038 40.0 37.0 41.0 33.0 41.0 20-21 37.98295753969379 40.0 37.0 41.0 33.0 41.0 22-23 38.00023051519982 40.0 37.0 41.0 33.0 41.0 24-25 37.92876552679813 40.0 37.0 41.0 33.0 41.0 26-27 37.74984170630055 40.0 37.0 41.0 32.0 41.0 28-29 37.60971666087356 39.5 37.0 41.0 32.0 41.0 30-31 37.34258961237171 39.0 36.0 41.0 31.5 41.0 32-33 37.095743284554686 39.0 36.0 41.0 31.0 41.0 34-35 36.904868289748705 39.0 35.0 40.5 31.0 41.0 36-37 36.89844420377939 39.0 35.0 41.0 31.0 41.0 38-39 36.74337095666776 39.0 35.0 41.0 30.0 41.0 40-41 36.54427507751444 39.0 35.0 41.0 30.0 41.0 42-43 36.282012427374525 39.0 35.0 40.0 30.0 41.0 44-45 35.93552681132512 38.0 35.0 40.0 29.5 41.0 46-47 35.495663577081444 38.0 35.0 40.0 27.5 41.0 48-49 35.042653061762906 38.0 34.0 40.0 26.0 41.0 50-51 34.651362249195174 38.0 34.0 40.0 24.5 41.0 52-53 34.14317945426928 38.0 33.0 40.0 23.5 41.0 54-55 33.81931301853554 37.0 33.0 40.0 23.0 41.0 56-57 33.451013541366436 37.0 32.5 40.0 22.0 41.0 58-59 33.00019951601702 36.5 32.0 39.0 20.5 41.0 60-61 32.594901524830014 36.0 31.0 39.0 19.5 40.0 62-63 32.11871170034475 35.0 31.0 39.0 18.0 40.0 64-65 31.62827231001293 35.0 30.5 38.5 14.0 40.0 66-67 31.142364736384927 35.0 30.0 38.0 10.5 40.0 68-69 30.592294295688674 34.0 29.0 37.5 8.0 39.5 70-71 30.041868188836467 34.0 29.0 37.0 7.0 39.0 72-73 29.474261609358848 34.0 28.0 36.0 2.0 39.0 74-75 28.87303839479636 33.5 27.0 35.5 2.0 38.5 76-77 27.6134665726578 31.5 26.0 34.5 2.0 36.0 78-79 28.231273855355177 33.0 27.0 35.0 2.0 37.0 80-81 27.955802090664037 33.0 26.0 35.0 2.0 36.5 82-83 27.48514182785692 33.0 26.0 35.0 2.0 36.0 84-85 26.927966968062265 32.5 25.0 35.0 2.0 36.0 86-87 26.31448687448431 32.0 24.5 35.0 2.0 35.0 88-89 25.59610109427115 31.5 21.5 34.0 2.0 35.0 90-91 25.080093643914367 31.0 19.5 34.0 2.0 35.0 92-93 24.333804806720096 31.0 16.5 34.0 2.0 35.0 94-95 23.574658398898805 31.0 4.5 34.0 2.0 35.0 96-97 22.63499979223952 30.0 2.0 34.0 2.0 35.0 98-99 21.497768883284117 30.0 2.0 34.0 2.0 35.0 100-101 18.387042110740953 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3482453415438691 1101 2 0.333967735027656 1101 3 0.2689383098428024 1101 4 0.20366642577324257 1101 5 0.17519767286305665 1101 6 0.2407471965796475 1101 7 0.2952290258174344 1101 8 0.2803523927267051 1101 9 0.3647727599496733 1101 10-11 0.40967512461070754 1101 12-13 0.39412119677626833 1101 14-15 0.5380166938199125 1101 16-17 0.5159001582933698 1101 18-19 0.5320498032015593 1101 20-21 0.6537494629741829 1101 22-23 0.6138077984469206 1101 24-25 0.5842139611691621 1101 26-27 0.625043340901712 1101 28-29 0.5819706761075594 1101 30-31 0.5545185466751406 1101 32-33 0.46528164078581113 1101 34-35 0.3795012145292418 1101 36-37 0.5173644404128126 1101 38-39 0.7501483339492196 1101 40-41 0.6466230629912246 1101 42-43 0.6190963018801341 1101 44-45 0.6778572242976608 1101 46-47 0.6872464091917578 1101 48-49 0.6683437013480926 1101 50-51 0.7033535470073673 1101 52-53 0.8446397080714121 1101 54-55 0.8135243262853677 1101 56-57 0.9571446946996716 1101 58-59 0.915495370130536 1101 60-61 1.1381903108384535 1101 62-63 1.123730379803284 1101 64-65 1.1098583396837718 1101 66-67 1.2850912199701376 1101 68-69 1.2616592107464122 1101 70-71 1.3405462319241757 1101 72-73 1.3473338659944147 1101 74-75 1.4369788983471246 1101 76-77 1.2529587516902545 1101 78-79 1.5230550343638747 1101 80-81 1.767506274116947 1101 82-83 1.9517104886014387 1101 84-85 2.1894069229711555 1101 86-87 2.094851749146283 1101 88-89 2.50858643845827 1101 90-91 2.6616617352064615 1101 92-93 2.961437378152201 1101 94-95 3.105230088945415 1101 96-97 3.7123842482555283 1101 98-99 4.096447162503377 1101 100-101 4.8394322627941335 1102 1 0.3195379321631613 1102 2 0.2844484175514097 1102 3 0.32644949915172816 1102 4 0.18930170730602924 1102 5 0.10242964916140807 1102 6 0.1615911823002918 1102 7 0.19374533240243608 1102 8 0.24926876098885486 1102 9 0.33127986452411307 1102 10-11 0.316501325835155 1102 12-13 0.33263747582682157 1102 14-15 0.521527693141195 1102 16-17 0.4291054014018485 1102 18-19 0.5260466064528231 1102 20-21 0.46306725586837416 1102 22-23 0.5293575993040704 1102 24-25 0.3328609875182451 1102 26-27 0.37847860673986844 1102 28-29 0.33057708915448103 1102 30-31 0.39908730912587487 1102 32-33 0.22924057041190338 1102 34-35 0.41577577697253787 1102 36-37 0.46944143221993784 1102 38-39 0.6413081259540405 1102 40-41 0.5797880232108454 1102 42-43 0.6198177042643422 1102 44-45 0.5248827473488049 1102 46-47 0.6057189910826324 1102 48-49 0.7529701209206721 1102 50-51 0.6424260217892623 1102 52-53 0.7459376816741496 1102 54-55 0.6674458503718128 1102 56-57 1.026584492707002 1102 58-59 0.9911971886427651 1102 60-61 1.0517080698652777 1102 62-63 1.2361335446120592 1102 64-65 1.0394555245624097 1102 66-67 1.014370519714202 1102 68-69 1.2689572146370303 1102 70-71 1.470488836379527 1102 72-73 1.5517144580675257 1102 74-75 1.6756102713658514 1102 76-77 1.6142725851316584 1102 78-79 1.9584964045878515 1102 80-81 2.065096459740392 1102 82-83 2.0558333582960664 1102 84-85 2.2196252848645877 1102 86-87 2.33031941476251 1102 88-89 2.447685759321253 1102 90-91 2.605812937842064 1102 92-93 2.783376959325796 1102 94-95 2.934155158091283 1102 96-97 3.4076344193132506 1102 98-99 3.7300029741292455 1102 100-101 4.201894970834051 1103 1 0.24040431218725544 1103 2 0.28524674851508536 1103 3 0.2695578009372497 1103 4 0.21638857370889752 1103 5 0.15499708439560322 1103 6 0.13720051188785476 1103 7 0.23134290222159848 1103 8 0.18453007965887736 1103 9 0.3031773298500653 1103 10-11 0.3972178680235814 1103 12-13 0.31635377248475294 1103 14-15 0.5014912632686972 1103 16-17 0.4853886265304581 1103 18-19 0.41729337315889126 1103 20-21 0.377336966827599 1103 22-23 0.3670341100303247 1103 24-25 0.31610710042800605 1103 26-27 0.3802820781412777 1103 28-29 0.37094044572755536 1103 30-31 0.4588755890420515 1103 32-33 0.4536804182972105 1103 34-35 0.47378842179269753 1103 36-37 0.5808012857010496 1103 38-39 0.5615844119913476 1103 40-41 0.5699705508615693 1103 42-43 0.6365155618680802 1103 44-45 0.754330810512954 1103 46-47 0.9034545076388127 1103 48-49 0.9525512558996354 1103 50-51 0.9191759975217124 1103 52-53 1.0907832901044046 1103 54-55 1.144956970618935 1103 56-57 1.0305074489511838 1103 58-59 1.253762603960439 1103 60-61 1.1623511384824852 1103 62-63 1.2834786391413004 1103 64-65 1.1400280959518305 1103 66-67 1.2944205251387153 1103 68-69 1.2577980680553296 1103 70-71 1.3138252174314218 1103 72-73 1.3893502849628199 1103 74-75 1.484467284116306 1103 76-77 1.2583616884314779 1103 78-79 1.3635200012098103 1103 80-81 1.6695058018899793 1103 82-83 1.8581267129848449 1103 84-85 2.0879734785024944 1103 86-87 2.270836798892109 1103 88-89 2.296711096069174 1103 90-91 2.4373499372881895 1103 92-93 2.602042344094915 1103 94-95 2.6999226407185724 1103 96-97 3.14437773574495 1103 98-99 3.4182019152076357 1103 100-101 4.098862812050054 1104 1 0.26100628645131607 1104 2 0.299106258474545 1104 3 0.22286182878847427 1104 4 0.19179290122519888 1104 5 0.09726748806209429 1104 6 0.16594963051997524 1104 7 0.1929317881725936 1104 8 0.19070516338882726 1104 9 0.16933610827418022 1104 10-11 0.2578625886299548 1104 12-13 0.3273185398036276 1104 14-15 0.4720205246824136 1104 16-17 0.44680674745413995 1104 18-19 0.5506994193425641 1104 20-21 0.4998856624031305 1104 22-23 0.4405787513136019 1104 24-25 0.48905902180999306 1104 26-27 0.4051449152644935 1104 28-29 0.4737299750771129 1104 30-31 0.5512980286683486 1104 32-33 0.5674567491627087 1104 34-35 0.6561131397549786 1104 36-37 0.5985030087752605 1104 38-39 0.6578600818309468 1104 40-41 0.699542646754395 1104 42-43 0.5701430517323516 1104 44-45 0.6954196303613571 1104 46-47 0.7364655908399413 1104 48-49 0.9280740172252067 1104 50-51 0.9292263456620304 1104 52-53 1.0372732425105369 1104 54-55 0.8910211121666265 1104 56-57 1.0332699864093229 1104 58-59 1.1963772919562103 1104 60-61 1.1647032890201174 1104 62-63 1.2329046483075246 1104 64-65 1.2606846048536475 1104 66-67 1.367158163954155 1104 68-69 1.5544059034158089 1104 70-71 1.5801222604421774 1104 72-73 1.7405836491440319 1104 74-75 1.8013383179608304 1104 76-77 1.6452794347737978 1104 78-79 1.7373026118214803 1104 80-81 1.9358496675695172 1104 82-83 2.0614665464063116 1104 84-85 2.1509499680142206 1104 86-87 2.3757746624563616 1104 88-89 2.454894596862406 1104 90-91 2.690126246119494 1104 92-93 3.0056344690728807 1104 94-95 3.2643644043357085 1104 96-97 3.5415028238817357 1104 98-99 3.806741929309524 1104 100-101 4.189979725499729 1105 1 0.2188089676410918 1105 2 0.1787896127546702 1105 3 0.20108501689069413 1105 4 0.1512512520141982 1105 5 0.06802401647991019 1105 6 0.13458093201417398 1105 7 0.15997287880239242 1105 8 0.18280734883778393 1105 9 0.16492418954774735 1105 10-11 0.2674448746235427 1105 12-13 0.25866934625524607 1105 14-15 0.3643487609622582 1105 16-17 0.4329225134086059 1105 18-19 0.32483187291050797 1105 20-21 0.27949570290027026 1105 22-23 0.2240421293334336 1105 24-25 0.1765180010028402 1105 26-27 0.23622603172866974 1105 28-29 0.38691074591917385 1105 30-31 0.38389909695490587 1105 32-33 0.4072988626943328 1105 34-35 0.3965627103456768 1105 36-37 0.5295256487696705 1105 38-39 0.6128729117429685 1105 40-41 0.46645456548083075 1105 42-43 0.5851645221973669 1105 44-45 0.49745452423093184 1105 46-47 0.5676741336412192 1105 48-49 0.5252221983451619 1105 50-51 0.5347488809224643 1105 52-53 0.5442912916656866 1105 54-55 0.6472270366185171 1105 56-57 0.7365791436499336 1105 58-59 0.8990659003907879 1105 60-61 0.783590315971658 1105 62-63 0.9214031122092194 1105 64-65 1.0873171456998953 1105 66-67 1.0546140884060442 1105 68-69 1.0491072370284336 1105 70-71 1.092099026955232 1105 72-73 1.3564844486133758 1105 74-75 1.2731391313947107 1105 76-77 1.2508875027810618 1105 78-79 1.4374466210380668 1105 80-81 1.7958665995622525 1105 82-83 1.916089242258849 1105 84-85 2.0900537070171517 1105 86-87 2.0789323918237663 1105 88-89 2.261799184210542 1105 90-91 2.356640175738267 1105 92-93 2.7455603697850712 1105 94-95 2.625533846452729 1105 96-97 3.1840444556370784 1105 98-99 3.555166555758344 1105 100-101 3.793674216492608 1106 1 0.14813072396382765 1106 2 0.21446857769694816 1106 3 0.17782825078845121 1106 4 0.10186878214822315 1106 5 0.08524294586806747 1106 6 0.12738026760181498 1106 7 0.16781228702566864 1106 8 0.11330572174876608 1106 9 0.27325501259598184 1106 10-11 0.29247653603628265 1106 12-13 0.09695340375279216 1106 14-15 0.044867923276584065 1106 16-17 -0.0390996697333037 1106 18-19 0.19436908594249047 1106 20-21 0.3404839102172943 1106 22-23 0.19332016426810839 1106 24-25 0.21138066365870856 1106 26-27 0.20683596736638066 1106 28-29 0.41364788088483095 1106 30-31 0.4063399320100771 1106 32-33 0.2980741483364966 1106 34-35 0.24155278234489685 1106 36-37 0.3107913734076604 1106 38-39 0.3425702031806992 1106 40-41 0.34942152093635315 1106 42-43 0.5565936239678564 1106 44-45 0.13473130331593808 1106 46-47 -0.0956644430059086 1106 48-49 0.33368836465190554 1106 50-51 0.1360271640571682 1106 52-53 0.4217058854025595 1106 54-55 0.5216901714908317 1106 56-57 0.3426973271652116 1106 58-59 0.7273308443654614 1106 60-61 0.5108857949128449 1106 62-63 0.5419992059227567 1106 64-65 0.438809209663372 1106 66-67 0.5484948972411168 1106 68-69 0.36432476207118114 1106 70-71 0.5212771666854792 1106 72-73 0.8788926146061549 1106 74-75 1.26151175599324 1106 76-77 1.1730691597389367 1106 78-79 1.4503934871415858 1106 80-81 1.5061947433230998 1106 82-83 1.7710298545704184 1106 84-85 1.6769088642741359 1106 86-87 1.9791649997907719 1106 88-89 2.286187540871847 1106 90-91 2.1685814605233915 1106 92-93 1.9455268521756004 1106 94-95 2.4953194716410003 1106 96-97 2.9023904146437687 1106 98-99 3.276498127649372 1106 100-101 3.77934443079317 1107 1 0.10522278944605645 1107 2 0.16815794941329898 1107 3 0.12678343094914624 1107 4 0.07531251905378866 1107 5 0.029598900910592363 1107 6 0.07409921297920619 1107 7 0.055575634513537864 1107 8 0.11881081285726935 1107 9 0.20646284253786007 1107 10-11 0.09414664049825205 1107 12-13 0.20414923943777552 1107 14-15 0.2631760332815247 1107 16-17 0.33995444236560957 1107 18-19 0.2540313845204736 1107 20-21 0.19337957664512118 1107 22-23 0.19555383314956742 1107 24-25 0.20494856352859614 1107 26-27 0.25328520566814916 1107 28-29 0.13185073759573385 1107 30-31 0.1530061327089669 1107 32-33 0.15342045630650603 1107 34-35 0.21305484999992785 1107 36-37 0.32316948922076705 1107 38-39 0.29650220657631365 1107 40-41 0.355512048211736 1107 42-43 0.46471242481312913 1107 44-45 0.3671730165460261 1107 46-47 0.3897780154647137 1107 48-49 0.3930208935236834 1107 50-51 0.513934010119371 1107 52-53 0.67705407161273 1107 54-55 0.6115546231067981 1107 56-57 0.6656553337181066 1107 58-59 0.6608041425130935 1107 60-61 0.7406086785390258 1107 62-63 0.7668336359738603 1107 64-65 0.7804571426806071 1107 66-67 1.099404288780601 1107 68-69 1.14143610671357 1107 70-71 1.0733386481571543 1107 72-73 1.1751359392841891 1107 74-75 1.2020528307961555 1107 76-77 1.1246378648914614 1107 78-79 1.357748111096864 1107 80-81 1.66601743129414 1107 82-83 1.7050546163443947 1107 84-85 1.9885199909688254 1107 86-87 2.1133415500547876 1107 88-89 2.3287877211213193 1107 90-91 2.373923042102188 1107 92-93 2.424206260495051 1107 94-95 2.5558111210160597 1107 96-97 2.844125660169272 1107 98-99 3.0698980738475434 1107 100-101 3.5477697871173994 1108 1 0.1778411211350246 1108 2 0.14455586678145949 1108 3 0.1801082557423257 1108 4 0.1570640040047948 1108 5 0.015550203241666338 1108 6 0.016330734473804398 1108 7 0.10048435640435827 1108 8 0.10656233799839043 1108 9 0.19131254378321216 1108 10-11 0.23660097203626407 1108 12-13 0.26465608572482324 1108 14-15 0.29826542931731126 1108 16-17 0.3022629818325413 1108 18-19 0.35557042063422983 1108 20-21 0.30504603010816567 1108 22-23 0.2821255825416884 1108 24-25 0.27712024587185624 1108 26-27 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-0.240700397899829 2316 56-57 -0.0689529084730296 2316 58-59 -0.17530060011207382 2316 60-61 0.18001834080877188 2316 62-63 0.18698479483634856 2316 64-65 0.18984130504223629 2316 66-67 0.37001156971261295 2316 68-69 0.4424792690368271 2316 70-71 0.3560371090751353 2316 72-73 0.5689505599431222 2316 74-75 0.7132594908332521 2316 76-77 0.7726484018146884 2316 78-79 0.7857079136175429 2316 80-81 0.934891589723609 2316 82-83 1.1571442730866046 2316 84-85 1.2256521153428324 2316 86-87 1.304417765036412 2316 88-89 1.582766979041974 2316 90-91 1.616014219203219 2316 92-93 1.5384459353979096 2316 94-95 2.2940550979285277 2316 96-97 2.206828027284285 2316 98-99 2.5316822538649504 2316 100-101 3.0228474422567437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 59.0 5 118.0 6 257.0 7 647.0 8 1471.0 9 2589.0 10 4384.0 11 6956.0 12 9817.0 13 13611.0 14 17970.0 15 24690.0 16 31147.0 17 40145.0 18 36745.0 19 28638.0 20 27843.0 21 29696.0 22 32291.0 23 36301.0 24 41957.0 25 48687.0 26 57001.0 27 68315.0 28 82153.0 29 97262.0 30 115165.0 31 137043.0 32 163772.0 33 199176.0 34 249217.0 35 318666.0 36 403291.0 37 450639.0 38 241859.0 39 12743.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.49004727708275 19.260398583798104 15.654333820526487 22.595220318592663 2 16.192944398162275 20.83601540783754 38.757705980847426 24.213334213152752 3 19.11450446124704 24.518490035405048 29.99261955139536 26.374385951952554 4 13.642014841353436 17.39786263932319 35.79241496165665 33.16770755766672 5 14.134136804183031 37.26480106404647 33.2670009913538 15.334061140416699 6 32.27193010805524 35.902923750584534 16.958597623352016 14.866548518008216 7 29.073012309313807 32.901114585511245 19.75168995013089 18.274183155044064 8 27.19301080552366 34.10183825154056 19.39315472087874 19.31199622205704 9 27.20000211058266 16.450837604515065 19.571894689839986 36.77726559506229 10-11 25.450658864546106 26.55792329219236 26.50289974270678 21.488518100554753 12-13 26.478215818948943 23.835766329479103 27.13381555750052 22.55220229407144 14-15 23.169811544755234 25.120055877675906 25.926990988471605 25.78314158909726 16-17 22.420521722842242 28.366445297325033 26.44833788317793 22.76469509665479 18-19 22.46217275250978 26.386685785027925 29.439412756757328 21.71172870570497 20-21 23.215073649441454 25.50360480922641 29.55618733795507 21.725134203377063 22-23 23.040373467601565 25.24309625114351 29.461062717942394 22.255467563312532 24-25 22.83817187643507 25.595863602568414 29.438127916411975 22.127836604584537 26-27 22.21455025312001 26.12794161930183 29.358441615838725 22.299066511739433 28-29 22.305099233660627 26.244221455523757 29.451697007391658 21.998982303423958 30-31 22.347080041868683 25.89414504583592 29.227810356233373 22.53096455606202 32-33 22.314683391352638 25.908065892694843 29.07761505696621 22.69963565898631 34-35 23.05655339013267 26.021433167878417 28.781882834962104 22.140130607026816 36-37 23.092404245074565 25.56940129673968 28.981802111983956 22.356392346201794 38-39 22.489528950361997 25.983193548929705 28.780404303396086 22.74687319731221 40-41 22.80341082612438 25.735115150835558 28.81030041786083 22.65117360517923 42-43 22.785737948834562 25.80182112033249 28.56460172000378 22.847839210829168 44-45 22.512839073987198 26.13192030004147 28.631930605928442 22.723310020042888 46-47 22.852456702536692 26.476921925234564 27.904154452489944 22.7664669197388 48-49 22.830271559436977 26.225819812962804 27.982665520796164 22.96124310680406 50-51 23.086690863617445 26.565524687551324 27.545082375381636 22.802702073449595 52-53 23.257070946576537 26.546232642930967 27.358262832177676 22.838433578314817 54-55 22.725896651362742 26.587966117233634 27.624097313689965 23.06203991771366 56-57 22.697667608294324 26.70886293018786 27.710911514481566 22.882557947036247 58-59 22.90254252659169 26.24941546753693 27.534628395647186 23.313413610224192 60-61 22.761924956914434 26.508687356092892 27.785903154595566 22.943484532397115 62-63 22.4956782522265 26.78698746643679 27.64086655247535 23.07646772886136 64-65 22.251114486577684 26.47105962461968 27.602018640402225 23.67580724840041 66-67 22.39290671572021 26.53320067420036 27.57195532284878 23.501937287230657 68-69 22.526632134399488 26.416458878361976 27.643270049826324 23.413638937412216 70-71 22.53886275210745 26.593605330276503 27.23939415724764 23.628137760368404 72-73 22.65793303021184 26.672410310527773 27.059389002708308 23.610267656552082 74-75 22.470334771387623 26.91466122839868 26.92305409225489 23.691949907958808 76-77 22.516635678476476 26.529133625605063 27.235024591585766 23.719206104332695 78-79 22.711666707339354 26.63477818106029 27.308169471872855 23.345385639727496 80-81 22.62351690345865 26.9947479469637 27.102437129370145 23.27929802020751 82-83 22.652509545553183 26.852701347029416 26.97359959529097 23.52118951212643 84-85 22.62940473426485 26.580721141669127 27.04548392713918 23.744390196926844 86-87 22.72167838994132 26.674107694951037 26.813903169455926 23.790310745651713 88-89 22.557889363184028 26.72226956379504 26.78039306989727 23.939448003123665 90-91 22.506285227904748 26.596073585520735 27.03404023700095 23.86360094957357 92-93 22.539330722690025 26.213435507771994 27.058379685195927 24.18885408434206 94-95 22.356495219200497 26.34181941459033 27.03430488289894 24.267380483310237 96-97 22.425897113052702 26.25032235852336 26.968398641576236 24.355381886847706 98-99 22.398426560627477 26.30432359453333 26.645001315816373 24.65224852902282 100-101 22.44857106722569 26.1366767409262 26.8764744301838 24.53827776166431 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1237.0 1 852.5 2 582.5 3 699.5 4 598.0 5 611.0 6 1132.0 7 2608.5 8 4098.5 9 4471.0 10 3836.5 11 3187.0 12 3287.5 13 3612.0 14 3941.0 15 4394.5 16 4821.5 17 5118.0 18 5434.5 19 5885.0 20 6479.0 21 7273.0 22 8270.5 23 9523.0 24 11176.5 25 13440.5 26 16315.0 27 20045.5 28 23712.5 29 27187.0 30 31778.5 31 37027.0 32 43218.5 33 46434.0 34 47598.0 35 50015.0 36 54205.5 37 60394.5 38 64992.5 39 68482.0 40 73037.0 41 81868.0 42 91323.0 43 98484.0 44 102449.0 45 106528.0 46 109059.5 47 112573.0 48 118044.5 49 119570.5 50 121029.0 51 118432.5 52 114290.0 53 113457.5 54 114361.0 55 108021.0 56 95431.5 57 85993.0 58 78764.5 59 69919.5 60 57975.0 61 47764.5 62 40782.5 63 32605.0 64 25396.5 65 20256.0 66 15341.5 67 12181.0 68 9551.0 69 6915.5 70 5314.0 71 4310.5 72 3626.0 73 3033.0 74 2366.5 75 1870.5 76 1271.0 77 728.5 78 442.0 79 270.5 80 148.5 81 91.5 82 49.0 83 25.0 84 12.0 85 4.5 86 3.5 87 3.0 88 2.0 89 1.0 90 1.0 91 0.5 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.595570810377999E-5 2 0.04659770777532057 3 6.595570810377999E-5 4 0.0 5 0.10378130670129782 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012366695269458747 26-27 0.012432650977562527 28-29 0.0 30-31 0.0 32-33 4.6168995672645993E-4 34-35 0.01632403775568555 36-37 0.0038913867781230193 38-39 0.013421986599119229 40-41 0.007518950723830919 42-43 0.006381214759040714 44-45 0.0029844957916960444 46-47 3.297785405188999E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6488927025944995E-4 68-69 1.6488927025944995E-4 70-71 0.0 72-73 1.6488927025944996E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0011872027458680397 84-85 0.004814766691575939 86-87 0.0016818705566463898 88-89 6.595570810377999E-5 90-91 9.728466945307548E-4 92-93 2.4733390538917495E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.420017161675249E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3032338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.67590364891479 #Duplication Level Percentage of deduplicated Percentage of total 1 67.68341989424597 27.530842662473916 2 15.294283659696722 12.442176170419915 3 6.307579598920726 7.696995000706801 4 3.3301656403838296 5.41829986892717 5 1.9485627715873972 3.962977577547566 6 1.2137069546431407 2.962117628704931 7 0.8435895162545531 2.401963611768339 8 0.5814593764631403 1.8921108458218212 9 0.42747631313423357 1.5649186792717273 >10 2.115277725606922 15.241564973639967 >50 0.1416809944171549 4.003164904452261 >100 0.09741521972548198 7.7567513059789315 >500 0.010743218039981538 3.027911036931196 >1k 0.004394952834461451 2.959051990536823 >5k 8.138801545298984E-5 0.28172619446056146 >10k+ 1.6277603090597968E-4 0.8574275483582303 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14319 0.47220989216901277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11581 0.381916527774938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8510 0.28064153798158387 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3669 0.12099574651638438 No Hit ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT 3371 0.11116834600892117 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3103 0.10233028112301465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.297785405188999E-5 2 0.0 0.0 0.0 0.0 3.297785405188999E-5 3 0.0 0.0 0.0 0.0 3.297785405188999E-5 4 0.0 0.0 0.0 0.0 3.297785405188999E-5 5 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 6 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 7 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 8 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 9 0.0 0.0 0.0 3.297785405188999E-5 3.297785405188999E-5 10-11 0.0 0.0 0.0 4.946678107783499E-5 3.297785405188999E-5 12-13 0.0 0.0 0.0 6.595570810377999E-5 2.1435605133728497E-4 14-15 0.0 0.0 0.0 9.893356215566998E-5 2.3084497836322996E-4 16-17 0.0 0.0 0.0 1.3191141620755997E-4 2.3084497836322996E-4 18-19 0.0 0.0 0.0 1.6488927025944995E-4 2.968006864670099E-4 20-21 0.0 0.0 0.0 3.297785405188999E-4 2.968006864670099E-4 22-23 0.0 0.0 0.0 5.936013729340198E-4 2.968006864670099E-4 24-25 0.0 0.0 0.0 0.0010552913296604798 2.968006864670099E-4 26-27 0.0 0.0 0.0 0.0021270715863469045 2.968006864670099E-4 28-29 0.0 0.0 0.0 0.006034947291495869 2.968006864670099E-4 30-31 0.0 0.0 0.0 0.01818728650961733 2.968006864670099E-4 32-33 0.0 0.0 0.0 0.04148614039727761 3.957342486226799E-4 34-35 0.0 0.0 0.0 0.07675595530577396 3.957342486226799E-4 36-37 0.0 0.0 0.0 0.13435177740739984 3.957342486226799E-4 38-39 0.0 0.0 0.0 0.22075375502335162 4.4520102970051494E-4 40-41 0.0 0.0 0.0 0.3149879729766273 4.6168995672645993E-4 42-43 0.0 0.0 0.0 0.41190988603513196 4.6168995672645993E-4 44-45 0.0 0.0 0.0 0.5305312270597803 4.6168995672645993E-4 46-47 0.0 0.0 0.0 0.6705881732181571 4.6168995672645993E-4 48-49 0.0 0.0 0.0 0.8164327327626406 4.6168995672645993E-4 50-51 0.0 0.0 0.0 0.9770678598493967 4.946678107783499E-4 52-53 0.0 0.0 0.0 1.1354440039335985 5.111567378042949E-4 54-55 0.0 0.0 0.0 1.3093197394221883 5.276456648302399E-4 56-57 0.0 0.0 0.0 1.511193673000833 5.276456648302399E-4 58-59 0.0 0.0 0.0 1.7271326613326088 5.276456648302399E-4 60-61 0.0 0.0 0.0 1.9606653348010679 5.606235188821299E-4 62-63 0.0 0.0 0.0 2.19564903384781 6.265792269859099E-4 64-65 0.0 0.0 0.0 2.4440052527125937 6.265792269859099E-4 66-67 0.0 0.0 0.0 2.726806840134576 6.265792269859099E-4 68-69 0.0 0.0 0.0 3.0375241810114835 6.265792269859099E-4 70-71 0.0 0.0 0.0 3.373964248048865 6.595570810377999E-4 72-73 0.0 0.0 0.0 3.713899967615747 7.090238621156349E-4 74-75 0.0 0.0 0.0 4.0708687488004305 7.255127891415799E-4 76-77 0.0 0.0 0.0 4.450559271426866 7.255127891415799E-4 78-79 0.0 0.0 0.0 4.867102545956289 7.420017161675249E-4 80-81 0.0 0.0 0.0 5.310967972567702 7.749795702194148E-4 82-83 0.0 0.0 0.0 5.767480406208016 8.244463512972498E-4 84-85 0.0 0.0 0.0 6.241124835028286 8.409352783231948E-4 86-87 0.0 0.0 0.0 6.719715941956339 8.904020594010299E-4 88-89 0.0 0.0 0.0 7.241277192714005 9.233799134529199E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6915 0.0 58.674664 1 GTATCAA 12740 0.0 42.152008 1 TATCAAC 17055 0.0 31.319485 2 ATCAACG 17445 0.0 30.551153 3 TCAACGC 17835 0.0 29.88309 4 CAACGCA 18125 0.0 29.378727 5 TGGTATC 2865 0.0 28.721561 2 GTGGTAT 2970 0.0 28.50738 1 AACGCAG 18745 0.0 28.428177 6 GTACATG 15220 0.0 25.56421 1 TACATGG 15360 0.0 24.990152 2 ACGCAGA 21790 0.0 24.433743 7 CGCAGAG 22090 0.0 24.03741 8 ACATGGG 15885 0.0 23.31542 3 GTAAGGT 1560 0.0 22.248013 4 GCAGAGT 23655 0.0 22.005398 9 GAGTACT 13705 0.0 21.884462 12-13 TAAGGTG 1765 0.0 21.818922 5 AGTACTT 14095 0.0 21.346325 12-13 GGACCGA 1615 0.0 21.173935 6 >>END_MODULE