FastQCFastQC Report
Thu 26 May 2016
SRR938624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025162
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140150.4632809747048257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107600.3556834311683143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.25638296395366594No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG35070.11592767593933812No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG32480.10736615096976623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66100.056.6314931
GTATCAA127400.046.375831
TATCAAC171700.034.2401282
ATCAACG174850.033.5417373
TCAACGC178500.032.8824964
CAACGCA180200.032.5981185
AACGCAG186650.031.5131786
ACGCAGA213650.027.3696047
CGCAGAG217550.026.747948
GTACATG153350.026.2762131
TACATGG153550.025.9066732
TGGTATC28350.025.6492332
GTGGTAT29800.025.2831171
GCAGAGT228750.024.5034399
GTAAGGT14600.024.0887854
ACATGGG160100.023.5109333
GAGTACT129050.022.93150712-13
TAAGGTA6850.022.8959884
AGTACTT132150.022.75302112-13
TAAGGTG17050.022.5782075
Produced by FastQC (version 0.11.5)