##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025162 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14664900590448 31.0 31.0 34.0 28.0 34.0 2 31.323697375545507 31.0 31.0 34.0 28.0 34.0 3 31.367872861023642 31.0 31.0 34.0 28.0 34.0 4 35.04230583353883 37.0 35.0 37.0 32.0 37.0 5 35.03205382058878 37.0 35.0 37.0 32.0 37.0 6 35.016797778102465 37.0 35.0 37.0 32.0 37.0 7 34.94514045859362 37.0 35.0 37.0 32.0 37.0 8 34.901532876586444 37.0 35.0 37.0 32.0 37.0 9 36.38109661565232 39.0 37.0 39.0 32.0 39.0 10-11 36.20761912915738 39.0 35.0 39.0 32.0 39.0 12-13 36.024866767465674 38.0 35.0 39.0 30.5 39.0 14-15 37.17214317778684 39.0 36.0 41.0 31.5 41.0 16-17 37.11209994704416 39.0 36.0 41.0 31.0 41.0 18-19 37.13135412252302 39.5 36.0 41.0 31.0 41.0 20-21 37.06662866319225 39.0 36.0 41.0 31.0 41.0 22-23 36.913470749665635 39.0 36.0 41.0 30.5 41.0 24-25 36.76737097054637 39.0 36.0 41.0 30.0 41.0 26-27 36.540165121735626 39.0 36.0 41.0 30.0 41.0 28-29 36.29651651713198 39.0 35.0 40.5 29.5 41.0 30-31 35.9915609478104 38.5 35.0 40.0 28.5 41.0 32-33 35.66306068898128 38.0 35.0 40.0 27.5 41.0 34-35 35.33634942525392 38.0 34.0 40.0 26.5 41.0 36-37 35.089246956030784 38.0 34.0 40.0 25.5 41.0 38-39 34.823207153864814 38.0 34.0 40.0 25.0 41.0 40-41 34.52106234310757 38.0 33.0 40.0 24.0 41.0 42-43 34.05961879066311 38.0 33.0 40.0 23.5 41.0 44-45 33.515844606007875 37.5 32.5 40.0 20.0 41.0 46-47 32.93704122291632 37.0 31.5 40.0 16.5 41.0 48-49 32.380769691011594 37.0 31.0 40.0 12.0 41.0 50-51 31.619183534633848 36.0 30.0 38.5 9.0 40.5 52-53 31.600252647626803 36.0 30.0 39.0 8.0 40.0 54-55 31.553568536164345 36.0 30.0 39.0 6.5 40.0 56-57 31.108953338697233 36.0 29.5 39.0 2.0 40.0 58-59 30.643610490942304 35.0 28.5 39.0 2.0 40.0 60-61 30.082563181740348 35.0 28.0 38.5 2.0 40.0 62-63 29.35124152028883 34.0 26.5 38.0 2.0 40.0 64-65 28.68066916746938 34.0 26.0 38.0 2.0 40.0 66-67 27.971079069484546 34.0 25.5 37.0 2.0 39.5 68-69 27.11325988492517 33.0 22.5 36.0 2.0 39.0 70-71 26.266129053584567 32.0 20.5 36.0 2.0 39.0 72-73 25.35560574937805 31.5 16.5 35.0 2.0 38.0 74-75 24.411960417326412 31.0 9.0 35.0 2.0 37.0 76-77 23.4528479797115 30.0 4.5 34.0 2.0 36.5 78-79 22.44967790154709 29.0 2.0 34.0 2.0 36.0 80-81 21.47602888704803 28.5 2.0 34.0 2.0 35.0 82-83 20.459238877124598 26.5 2.0 33.5 2.0 35.0 84-85 19.46620941291739 26.0 2.0 33.0 2.0 35.0 86-87 18.45616499215579 24.5 2.0 32.0 2.0 34.5 88-89 17.35338239737244 21.0 2.0 32.0 2.0 34.0 90-91 16.189034174037623 14.0 2.0 31.0 2.0 34.0 92-93 15.02700714870807 3.5 2.0 31.0 2.0 34.0 94-95 13.770459896032015 2.0 2.0 30.0 2.0 34.0 96-97 12.369722679314364 2.0 2.0 29.0 2.0 34.0 98-99 10.715660681973395 2.0 2.0 26.5 2.0 33.5 100-101 7.545888947434881 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5834961142107247 1101 2 0.5723488395543512 1101 3 0.5805609197100914 1101 4 0.4016662593681204 1101 5 0.36519374804757376 1101 6 0.5237543498899981 1101 7 0.3981372349251018 1101 8 0.3908373095119444 1101 9 0.5580079264721007 1101 10-11 0.5923777933477226 1101 12-13 0.5835252931579973 1101 14-15 0.6475952467412966 1101 16-17 0.7478526995335315 1101 18-19 0.6598470375625567 1101 20-21 0.8285833427091873 1101 22-23 0.8279148909652037 1101 24-25 0.879719911971641 1101 26-27 0.8648991999850182 1101 28-29 1.0081591910025622 1101 30-31 0.9777457765836104 1101 32-33 0.9237992216722972 1101 34-35 0.9955802818530302 1101 36-37 1.2029870426471518 1101 38-39 1.142888129806174 1101 40-41 1.0923007311199981 1101 42-43 1.2763291942328365 1101 44-45 1.3176477439855887 1101 46-47 1.4280540478380388 1101 48-49 1.1465193975697971 1101 50-51 1.1969578648849932 1101 52-53 1.2390659467980534 1101 54-55 1.4689622212475655 1101 56-57 1.6776610271871384 1101 58-59 1.711511986612237 1101 60-61 1.8340458695922273 1101 62-63 1.6204448344141262 1101 64-65 1.8388807192119643 1101 66-67 2.172345409793987 1101 68-69 2.1904756805890457 1101 70-71 2.5039836122687866 1101 72-73 2.7039908764635996 1101 74-75 2.748754835106631 1101 76-77 2.9621696495081373 1101 78-79 3.2020409215520615 1101 80-81 3.4890835568949434 1101 82-83 3.498748723325164 1101 84-85 3.540191828658667 1101 86-87 3.723375440793241 1101 88-89 4.087675272105372 1101 90-91 4.142566165369441 1101 92-93 4.183746569394133 1101 94-95 4.050767377217381 1101 96-97 3.828589022345634 1101 98-99 3.6632949717509504 1101 100-101 2.641253062274516 1102 1 0.6652935189398121 1102 2 0.6008177559768875 1102 3 0.5417412073152903 1102 4 0.4570924638983982 1102 5 0.49673622520405303 1102 6 0.6228605752233278 1102 7 0.5789368998316249 1102 8 0.6067292228265444 1102 9 0.6309586479902833 1102 10-11 0.8238171320591832 1102 12-13 0.8027353859450201 1102 14-15 0.837448523096711 1102 16-17 0.9268476921707887 1102 18-19 0.8555777272248122 1102 20-21 0.916643771266088 1102 22-23 1.0148962238335741 1102 24-25 1.0060399563033826 1102 26-27 0.9518693722565388 1102 28-29 0.9860430466418038 1102 30-31 0.8186797143678533 1102 32-33 1.053226383684077 1102 34-35 1.0480059643028596 1102 36-37 1.2167892312765147 1102 38-39 1.417872209358812 1102 40-41 1.4425318469141288 1102 42-43 1.4249409337277257 1102 44-45 1.5598351382981193 1102 46-47 1.5510029906752365 1102 48-49 1.669454674625662 1102 50-51 1.6696906228275594 1102 52-53 1.6594266958682518 1102 54-55 1.7166280782385996 1102 56-57 1.92061983043045 1102 58-59 2.0181656062781315 1102 60-61 2.0391344879879973 1102 62-63 2.1229917928445943 1102 64-65 2.2868845506411084 1102 66-67 2.5112144492711828 1102 68-69 2.623821364481863 1102 70-71 2.718693642175676 1102 72-73 2.878253575619503 1102 74-75 2.9336428358241164 1102 76-77 3.026359114606155 1102 78-79 3.5012915958224653 1102 80-81 3.739523592548725 1102 82-83 4.086550303695741 1102 84-85 4.180937038370164 1102 86-87 4.11096518276425 1102 88-89 4.294623238421661 1102 90-91 4.215149939991022 1102 92-93 4.166010552206563 1102 94-95 4.155373234038072 1102 96-97 3.8719234529497673 1102 98-99 3.408724513186952 1102 100-101 2.7609393154755155 1103 1 0.46496254491537314 1103 2 0.3915293465101186 1103 3 0.44740009825190796 1103 4 0.3642382888771536 1103 5 0.3719647847612819 1103 6 0.43882905246292125 1103 7 0.379478541947293 1103 8 0.4046600138585674 1103 9 0.5002052085580218 1103 10-11 0.6148362434746417 1103 12-13 0.5857786796734246 1103 14-15 0.6196353066237279 1103 16-17 0.5499930360223289 1103 18-19 0.7006761170170037 1103 20-21 0.5923341671078362 1103 22-23 0.7377467988457482 1103 24-25 0.7672786441435093 1103 26-27 0.7425026046834518 1103 28-29 0.6857913114229603 1103 30-31 0.6394589812110496 1103 32-33 0.8293373677171161 1103 34-35 0.8655606050571834 1103 36-37 1.0115178962763736 1103 38-39 1.0831295159053766 1103 40-41 1.1379157642355366 1103 42-43 1.1999792743069193 1103 44-45 1.2103698675277315 1103 46-47 1.4408863860918402 1103 48-49 1.2758257134695654 1103 50-51 1.49299538531141 1103 52-53 1.5218541708865523 1103 54-55 1.6804324843408551 1103 56-57 1.69744605085042 1103 58-59 1.6603263934929942 1103 60-61 1.7634546846662396 1103 62-63 2.0468796506316913 1103 64-65 2.118486465401965 1103 66-67 2.3323070278959115 1103 68-69 2.423547816875601 1103 70-71 2.394607711503358 1103 72-73 2.725997742856812 1103 74-75 2.8650109068321186 1103 76-77 2.9162469738409733 1103 78-79 3.1035992933271146 1103 80-81 3.230052335436177 1103 82-83 3.3517142561196422 1103 84-85 3.576047504600293 1103 86-87 3.6336933767709603 1103 88-89 3.921989544653105 1103 90-91 3.989057245548306 1103 92-93 3.819365449401209 1103 94-95 3.6365155323591463 1103 96-97 3.598283674876333 1103 98-99 3.2359372278785123 1103 100-101 2.591717354165479 1104 1 0.5026295936725411 1104 2 0.4805951956997525 1104 3 0.4197592804991288 1104 4 0.29686864801848856 1104 5 0.3316340645834117 1104 6 0.45521453058290007 1104 7 0.40191892831413156 1104 8 0.4994359744556647 1104 9 0.6477535063906146 1104 10-11 0.55034719882633 1104 12-13 0.6289061418319406 1104 14-15 0.6406322023220596 1104 16-17 0.5563506444770923 1104 18-19 0.7740353457117806 1104 20-21 0.6412589852362558 1104 22-23 0.7095981657544499 1104 24-25 0.6925509641448073 1104 26-27 0.762449570838541 1104 28-29 0.8571016603345285 1104 30-31 0.6977915206414664 1104 32-33 0.7509554175773872 1104 34-35 0.7997489916080056 1104 36-37 0.7781700523189201 1104 38-39 0.8976331107316824 1104 40-41 0.8359512585330506 1104 42-43 1.037952504013603 1104 44-45 0.9978442606270974 1104 46-47 1.182583602481749 1104 48-49 1.0816137234222296 1104 50-51 1.2354868436506052 1104 52-53 1.3190870717151348 1104 54-55 1.5966675899028218 1104 56-57 1.845309197583223 1104 58-59 1.9203839003168781 1104 60-61 1.9729104017786874 1104 62-63 2.0742208194760217 1104 64-65 2.156445986863279 1104 66-67 2.3051405176931006 1104 68-69 2.590529501959118 1104 70-71 2.5455664580476203 1104 72-73 2.8696896502116758 1104 74-75 2.4899075440627882 1104 76-77 2.816985829054442 1104 78-79 2.894923183227643 1104 80-81 3.223956460668738 1104 82-83 3.360912511364006 1104 84-85 3.431571808822085 1104 86-87 3.516640952394873 1104 88-89 3.599518554882753 1104 90-91 3.7055217975288315 1104 92-93 3.8088302729473877 1104 94-95 3.8735080970204177 1104 96-97 3.894422402319462 1104 98-99 3.5556533462775217 1104 100-101 2.4848207280098977 1105 1 0.505602563718405 1105 2 0.4678286350234444 1105 3 0.3949312007353889 1105 4 0.3942367679787111 1105 5 0.39067548038841693 1105 6 0.4363124460081522 1105 7 0.414853733895832 1105 8 0.45411086128174816 1105 9 0.5117815982499749 1105 10-11 0.5299808795905747 1105 12-13 0.6066624818178994 1105 14-15 0.654765685835514 1105 16-17 0.7600330311227523 1105 18-19 0.6788188625578826 1105 20-21 0.5792975713313879 1105 22-23 0.6850747282955041 1105 24-25 0.7680980737370149 1105 26-27 0.5415025117948744 1105 28-29 0.5662151726737292 1105 30-31 0.7448466396669673 1105 32-33 0.8494919228079425 1105 34-35 0.8569991728546213 1105 36-37 0.9485143195694619 1105 38-39 0.9202415694507167 1105 40-41 0.9998537511204617 1105 42-43 1.1894650836176197 1105 44-45 1.3241859762168602 1105 46-47 1.4804073240697804 1105 48-49 1.410554120439457 1105 50-51 1.3399667154370505 1105 52-53 1.3920301919622027 1105 54-55 1.5355304044653053 1105 56-57 1.6560860075804733 1105 58-59 1.8054305419008685 1105 60-61 2.0762280004424234 1105 62-63 2.024876325861559 1105 64-65 2.1341533760529927 1105 66-67 2.3825789616647057 1105 68-69 2.5363108386986717 1105 70-71 2.7844426978887213 1105 72-73 2.8372551635875745 1105 74-75 2.8454287595952366 1105 76-77 3.088287923451521 1105 78-79 3.466121285330747 1105 80-81 3.6116161525886206 1105 82-83 3.7825339761970156 1105 84-85 3.4859543741461962 1105 86-87 3.775599993606942 1105 88-89 3.7541494556921435 1105 90-91 3.9155670906872686 1105 92-93 3.7827829269651954 1105 94-95 3.6938263983666655 1105 96-97 3.7212558241002167 1105 98-99 3.45386678697591 1105 100-101 2.4369309255277 1106 1 0.2808191111457994 1106 2 0.2667697711029682 1106 3 0.1791863219858989 1106 4 0.28942683338538444 1106 5 0.2804932643243916 1106 6 -0.04558945669919012 1106 7 0.3330574549282517 1106 8 0.04779027999472163 1106 9 0.32531077568764033 1106 10-11 0.3504883738315101 1106 12-13 0.5442528076255542 1106 14-15 0.6413900661793619 1106 16-17 0.1892556929285476 1106 18-19 0.5422017364199547 1106 20-21 0.5369783474980707 1106 22-23 0.34131786884969983 1106 24-25 0.5043472886464073 1106 26-27 0.4275402916159692 1106 28-29 0.5228352296776038 1106 30-31 0.4592726808487626 1106 32-33 0.732985571312895 1106 34-35 0.5862133714201647 1106 36-37 0.5893181960670333 1106 38-39 0.7018938274161215 1106 40-41 0.9075207901558002 1106 42-43 0.37510844725949966 1106 44-45 0.8319030025677563 1106 46-47 0.9644667797917137 1106 48-49 0.8384480952169113 1106 50-51 0.8140777468954994 1106 52-53 1.0122981514895706 1106 54-55 0.9151263764632347 1106 56-57 1.4667956857126008 1106 58-59 1.439474092567849 1106 60-61 1.7993609648257305 1106 62-63 1.810714760937561 1106 64-65 1.7240847362562093 1106 66-67 2.1725402037765384 1106 68-69 2.2214448445191266 1106 70-71 2.200512264889756 1106 72-73 2.393953557677314 1106 74-75 2.683384859975938 1106 76-77 2.7225926672227523 1106 78-79 3.037134072536329 1106 80-81 2.7911872976921224 1106 82-83 3.075903392256368 1106 84-85 3.08592712918313 1106 86-87 2.815704652203788 1106 88-89 2.7103683947144965 1106 90-91 2.5742813514763867 1106 92-93 2.7780762397338297 1106 94-95 2.5592076704667175 1106 96-97 2.2964631265841344 1106 98-99 2.2474966665498375 1106 100-101 1.6334797787482254 1107 1 0.15930134091336257 1107 2 0.06766440110529004 1107 3 -0.010862260727876105 1107 4 0.07219125632903456 1107 5 0.030194473506476527 1107 6 0.1390595066610132 1107 7 0.05535267530479615 1107 8 0.19678195503703932 1107 9 0.14968632465884468 1107 10-11 0.158858912462847 1107 12-13 0.2027636135964599 1107 14-15 0.2747953152350675 1107 16-17 0.26426555361955906 1107 18-19 0.4220131794152593 1107 20-21 0.42587538762751365 1107 22-23 0.4672443381916622 1107 24-25 0.41391386177510725 1107 26-27 0.37681535262156984 1107 28-29 0.4598767966483379 1107 30-31 0.41588504639289425 1107 32-33 0.4621808993254177 1107 34-35 0.6012670626847054 1107 36-37 0.5225123114917594 1107 38-39 0.6414419503603241 1107 40-41 0.5987947320323741 1107 42-43 0.7872176134252129 1107 44-45 0.6287580163529256 1107 46-47 0.6296304831029218 1107 48-49 0.7344870081173127 1107 50-51 0.9162724238648039 1107 52-53 0.9922765926133259 1107 54-55 1.0697733954060595 1107 56-57 1.2975279152821635 1107 58-59 1.1424683963357438 1107 60-61 1.3063996765521928 1107 62-63 1.628562948273263 1107 64-65 1.6084455615128839 1107 66-67 1.7791403127589547 1107 68-69 1.7930085125060984 1107 70-71 1.9412397502393048 1107 72-73 1.9158972582798057 1107 74-75 2.119409270928351 1107 76-77 2.2033399650370065 1107 78-79 2.5911251179200576 1107 80-81 2.7335396783955836 1107 82-83 2.775650994207595 1107 84-85 2.907459809255581 1107 86-87 2.893149489755835 1107 88-89 2.899591392063595 1107 90-91 2.7049281811256236 1107 92-93 2.9021322468747126 1107 94-95 3.072697313573064 1107 96-97 3.001704103655852 1107 98-99 2.6915277407949 1107 100-101 1.766824933030418 1108 1 0.2175078022830661 1108 2 0.20926586512358725 1108 3 0.0727752953977614 1108 4 0.08635692297808362 1108 5 0.04821559702767786 1108 6 0.105026011038035 1108 7 -0.018378145819319514 1108 8 0.12795328214195933 1108 9 0.20473303026152934 1108 10-11 0.19495542475378613 1108 12-13 0.10514511760046474 1108 14-15 0.1775873231917302 1108 16-17 0.31152903867440074 1108 18-19 0.23345793589269448 1108 20-21 0.25863396856595955 1108 22-23 0.3224415525380806 1108 24-25 0.34870821429041143 1108 26-27 0.2770817303234452 1108 28-29 0.3306303300751594 1108 30-31 0.3543881802812834 1108 32-33 0.400036130514124 1108 34-35 0.44120674484592826 1108 36-37 0.5225802133442059 1108 38-39 0.6004303092929817 1108 40-41 0.7170948014244161 1108 42-43 0.8503609940208605 1108 44-45 0.8662564371310921 1108 46-47 0.9818743183270087 1108 48-49 0.9623573304810051 1108 50-51 0.8851208440619018 1108 52-53 1.0299730393735906 1108 54-55 1.203876086250098 1108 56-57 1.4582506627869911 1108 58-59 1.4543116134421012 1108 60-61 1.475600314763998 1108 62-63 1.6835586220489596 1108 64-65 1.7801677999178374 1108 66-67 1.9578871829006204 1108 68-69 2.19268415815009 1108 70-71 2.3015809165129006 1108 72-73 2.2748674538183984 1108 74-75 2.3100700342107814 1108 76-77 2.5537109931300996 1108 78-79 2.8509684028814064 1108 80-81 2.8511344401402106 1108 82-83 3.0468263907375466 1108 84-85 2.988230445139326 1108 86-87 3.0011587967614304 1108 88-89 3.1833151821359955 1108 90-91 2.97699145646731 1108 92-93 3.0366807281413006 1108 94-95 2.877071326910885 1108 96-97 2.7677718656745256 1108 98-99 2.5769330319096824 1108 100-101 1.8304946115589624 1109 1 0.6671393708507551 1109 2 0.561219179213623 1109 3 0.46357795599262985 1109 4 0.4960562614150703 1109 5 0.4728852637361811 1109 6 0.5353288240254699 1109 7 0.47538272335614096 1109 8 0.5006875632972694 1109 9 0.6289706192318931 1109 10-11 0.6366823014233844 1109 12-13 0.5993766108682976 1109 14-15 0.7626913983481529 1109 16-17 0.818735414062111 1109 18-19 0.7422877402923547 1109 20-21 0.7822745372591484 1109 22-23 0.9167366921117406 1109 24-25 0.7161293735496059 1109 26-27 0.7273739654741362 1109 28-29 0.7203752069957616 1109 30-31 0.6402578039612976 1109 32-33 0.8146532666096391 1109 34-35 0.792355964058153 1109 36-37 0.8427229244975436 1109 38-39 0.9865132825615319 1109 40-41 0.956895256809247 1109 42-43 1.1217864919859721 1109 44-45 1.0600836418929163 1109 46-47 1.126799645971026 1109 48-49 1.0396463564235958 1109 50-51 0.9951939924922613 1109 52-53 1.0566973164053373 1109 54-55 1.1667640149662333 1109 56-57 1.3776860931615182 1109 58-59 1.3620657056306342 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-1.568376144769708 2316 46-47 -1.1271272897667757 2316 48-49 -0.9103329117972159 2316 50-51 -0.829165532339573 2316 52-53 -0.9105513134029586 2316 54-55 -1.0013507191197561 2316 56-57 -1.0090369386875473 2316 58-59 -0.6152433094309515 2316 60-61 -0.7729714684366336 2316 62-63 -0.7754310004253462 2316 64-65 -0.5627921271413747 2316 66-67 -0.49921393976167394 2316 68-69 -0.2811945607290127 2316 70-71 -0.38649792494766544 2316 72-73 -0.3068707631960308 2316 74-75 -0.2742732242907593 2316 76-77 -0.23503653095422905 2316 78-79 -0.3436937695023907 2316 80-81 -0.392233007250983 2316 82-83 -0.266711482336877 2316 84-85 -0.2628637872285573 2316 86-87 -0.07934581913886163 2316 88-89 -0.500480770154379 2316 90-91 -0.47792560452901967 2316 92-93 -0.46638830420670985 2316 94-95 -0.49735951838211356 2316 96-97 -0.3897416994883134 2316 98-99 -0.6299072436279367 2316 100-101 -0.10982256519638955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9856.0 3 3216.0 4 3760.0 5 4305.0 6 5309.0 7 6705.0 8 8885.0 9 11588.0 10 14849.0 11 19316.0 12 24552.0 13 29892.0 14 34883.0 15 41062.0 16 49860.0 17 58316.0 18 55830.0 19 51946.0 20 56472.0 21 61833.0 22 68912.0 23 76475.0 24 85306.0 25 95028.0 26 104486.0 27 115097.0 28 127432.0 29 140198.0 30 155443.0 31 171541.0 32 192440.0 33 215141.0 34 237293.0 35 253299.0 36 239539.0 37 158492.0 38 36149.0 39 456.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.580910219225174 18.49754757179741 15.05399324337778 22.867548965599635 2 15.68331869071517 20.11237727231996 39.73022312440296 24.47408091256191 3 19.059144016221815 24.508874776956663 29.450185775754314 26.98179543106721 4 13.13497525346759 16.680908958026468 36.18992690794342 33.99418888056252 5 14.242487289311512 37.16684368408362 32.985075959821955 15.605593066782921 6 32.2021789841572 35.38251289308915 17.328384574398243 15.08692354835541 7 29.105003500304065 32.850775275188056 19.802329545773958 18.241891678733925 8 27.234114601590225 33.70652902870794 19.57160979003774 19.487746579664098 9 27.590515947181782 16.42067677477127 19.518879616733265 36.46992766131368 10-11 25.443988816926527 26.326204281068883 26.52148975152235 21.708317150482245 12-13 26.57326529023055 23.773687410091625 27.057251535458516 22.595795764219307 14-15 23.350573549659362 24.936713129942426 25.90387141060283 25.808841909795376 16-17 22.523725015477776 28.20571020475302 26.378447990120673 22.89211678964853 18-19 22.546563067169387 26.301730725292373 29.323514989556394 21.828191217981846 20-21 23.328977788374083 25.447697168873734 29.426580904922744 21.79674413782944 22-23 23.071880317631006 25.181903074529004 29.257659538023727 22.48855706981626 24-25 22.750424953536207 25.474509401890298 29.35951069163255 22.415554952940944 26-27 22.212233799203158 25.968942709899768 29.267690600357497 22.551132890539577 28-29 22.290366841803575 26.217282522632807 29.332497411303564 22.159853224260058 30-31 22.35514814166324 25.784035034376167 29.118249617119453 22.74256720684114 32-33 22.308705516529784 25.83815306997509 28.970465235815357 22.88267617767977 34-35 23.043337944972475 26.014455901531363 28.593143647709567 22.349062505786595 36-37 23.05713119865933 25.525335613634454 28.825274571931004 22.59225861577521 38-39 22.444332807825713 25.968854044598555 28.649339627522714 22.937473520053018 40-41 22.775684236052328 25.728710383256164 28.72496283612172 22.770642544569792 42-43 22.78145824541289 25.749123523039824 28.39615089041199 23.0732673411353 44-45 22.46723939458256 26.084873754554817 28.478320377199058 22.969566473663576 46-47 22.820459835480204 26.43696934430132 27.748796519163115 22.99377430105536 48-49 22.860463213299468 26.205299321487818 27.82584330493043 23.108394160282284 50-51 23.13078145148453 26.53500786081522 27.366993039405568 22.967217648294678 52-53 23.124281481542702 26.526193122884735 27.289609944171477 23.059915451401086 54-55 22.627425460314512 26.559171652894104 27.591068246369193 23.222334640422186 56-57 22.64891372873517 26.604430774154025 27.65866780566163 23.087987691449175 58-59 22.799776532249933 26.07762859726054 27.61732470095486 23.50527016953467 60-61 22.68144604760102 26.34770664493617 27.844185081662914 23.126662225799894 62-63 22.463435240194862 26.557765964709457 27.739396050224073 23.239402744871608 64-65 22.2498169382152 26.270167788729342 27.710843373493976 23.769171899561478 66-67 22.423393954820657 26.340512817122335 27.592076838272362 23.644016389784646 68-69 22.52478565599736 26.170687429741395 27.725273393872467 23.579253520388775 70-71 22.518292849237827 26.383094787250837 27.3342155006327 23.764396862878638 72-73 22.5879001473963 26.40443448468406 27.23072653300333 23.77693883491631 74-75 22.401320944219233 26.63977458389067 27.167229898380256 23.791674573509837 76-77 22.572564109557117 26.17906295790327 27.1968819808942 24.051490951645416 78-79 22.702257476489994 26.310811463058727 27.23007046225274 23.756860598198543 80-81 22.67606651213311 26.639929925980265 26.931717668085 23.752285893801623 82-83 22.68880822838523 26.39936404640517 26.847285510301848 24.06454221490775 84-85 22.7154428944404 26.08131997642899 26.938074863569984 24.265162265560626 86-87 22.810153588412675 26.15363204762292 26.69087084120911 24.345343522755297 88-89 22.703823129076273 25.96831256577166 26.852326651279856 24.47553765387221 90-91 22.643158609454527 25.942081050290778 26.87470148855229 24.540058851702405 92-93 22.750714210503784 25.41896795652403 26.9511463981525 24.879171434819686 94-95 22.540614762824863 25.519471545199295 26.94135193862423 24.998561753351613 96-97 22.6565934106808 25.39100928675469 26.784163005961332 25.168234296603174 98-99 22.660434006205662 25.343471021484433 26.390277631533927 25.60581734077598 100-101 23.42441678623025 24.39156617533896 26.59966141579828 25.584355622632515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 925.0 1 702.5 2 566.0 3 649.0 4 571.0 5 561.0 6 910.5 7 1989.0 8 3318.0 9 3883.5 10 3415.0 11 2842.0 12 2908.5 13 3248.5 14 3672.0 15 4097.5 16 4571.0 17 5020.0 18 5430.5 19 5894.5 20 6503.0 21 7414.5 22 8636.5 23 9972.0 24 11577.0 25 13747.5 26 16613.0 27 20362.5 28 23863.5 29 27227.0 30 31404.0 31 36581.5 32 42107.5 33 45534.5 34 47467.5 35 49937.0 36 54231.5 37 59765.5 38 64088.0 39 67972.0 40 72809.0 41 80340.0 42 88589.0 43 94578.0 44 98679.5 45 102964.5 46 106146.0 47 109590.5 48 114185.5 49 116571.0 50 116939.5 51 114808.0 52 112979.5 53 112500.5 54 111769.5 55 105949.5 56 95551.0 57 87115.5 58 80623.0 59 72256.5 60 61671.0 61 51982.5 62 43830.0 63 35787.5 64 28843.0 65 23771.5 66 18923.5 67 14901.5 68 11647.5 69 8829.0 70 6978.0 71 5552.5 72 4503.5 73 3682.5 74 2886.0 75 2294.0 76 1645.0 77 1052.0 78 684.0 79 416.5 80 237.5 81 134.0 82 74.0 83 48.0 84 29.0 85 23.0 86 16.5 87 14.5 88 12.5 89 7.5 90 5.0 91 4.5 92 3.5 93 1.5 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3907889891516554 2 0.029254631652784215 3 0.035898903926467406 4 0.043105129576531766 5 0.06343461936914453 6 0.054311141023191484 7 0.037650876217538105 8 0.0393367363466816 9 0.034841109335632274 10-11 0.03727073128645673 12-13 0.029833113069647182 14-15 0.027700995847495108 16-17 0.02196576580031086 18-19 0.02720515463304114 20-21 0.034626244809368885 22-23 0.03384942690672433 24-25 0.04303901741460457 26-27 0.03654349750525757 28-29 0.03161814144168147 30-31 0.034593188728405284 32-33 0.029039767126520826 34-35 0.03074215529614612 36-37 0.03305608096359798 38-39 0.015453717850482057 40-41 0.012677007049539826 42-43 0.013569521235556973 44-45 0.011239067527623313 46-47 0.019899760740085985 48-49 0.01750319487022513 50-51 0.011520044215813896 52-53 0.009073894224507646 54-55 0.008148323957526902 56-57 0.009024310103062249 58-59 0.003867561472740964 60-61 0.007503730378736742 62-63 0.011817548944486278 64-65 0.007503730378736742 66-67 0.0035370006631049843 68-69 0.005834398290075044 70-71 0.013701745559411364 72-73 0.010693642191723947 74-75 0.01176796482304088 76-77 0.005437725318511868 78-79 0.009255702669807435 80-81 0.011916717187377072 82-83 0.013652161437965965 84-85 0.008578053010053675 86-87 0.0021155891816702706 88-89 0.016296647915053804 90-91 0.025800271192088223 92-93 0.010528361786905957 94-95 0.021354228302484297 96-97 0.01935433540418662 98-99 0.011354763810995906 100-101 0.12419169618023762 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025162.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.50451081263256 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2826213829543 30.57611061894896 2 14.20678612783195 12.361185614220467 3 5.9230911785004015 7.730435526578379 4 2.9913996765698836 5.205575182969603 5 1.7857389179009489 3.88438490311803 6 1.05493765912095 2.753672807872839 7 0.7455647030639423 2.270479938017372 8 0.5308113879134092 1.8474151811957928 9 0.38221171256190595 1.4965140223677822 >10 1.8733156308686847 14.398571165975502 >50 0.12475140977714848 3.757940340364325 >100 0.08619222185820695 7.476605832253669 >500 0.00881993276839167 2.6348983280592515 >1k 0.003527973107283263 2.520787244291736 >5k 7.669506754963614E-5 0.25878525303410593 >10k+ 1.5339013509927228E-4 0.8266380407323329 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14015 0.4632809747048257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10760 0.3556834311683143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.25638296395366594 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3507 0.11592767593933812 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3248 0.10736615096976623 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.916824289079395E-5 14-15 0.0 0.0 0.0 0.0 9.916824289079395E-5 16-17 0.0 0.0 0.0 3.3056080963597985E-5 9.916824289079395E-5 18-19 0.0 0.0 0.0 9.916824289079395E-5 9.916824289079395E-5 20-21 0.0 0.0 0.0 1.3222432385439194E-4 9.916824289079395E-5 22-23 0.0 0.0 0.0 4.7931317397217075E-4 9.916824289079395E-5 24-25 0.0 0.0 0.0 0.0011569628337259293 1.1569628337259295E-4 26-27 0.0 0.0 0.0 0.002148645262633869 1.3222432385439194E-4 28-29 0.0 0.0 0.0 0.006115374978265626 1.3222432385439194E-4 30-31 0.0 0.0 0.0 0.019238639120814027 1.3222432385439194E-4 32-33 0.0 0.0 0.0 0.04322082585990436 1.983364857815879E-4 34-35 0.0 0.0 0.0 0.0788222250576994 3.1403276915418083E-4 36-37 0.0 0.0 0.0 0.13495145053388877 3.305608096359798E-4 38-39 0.0 0.0 0.0 0.21891389618142765 3.305608096359798E-4 40-41 0.0 0.0 0.0 0.31323942321105447 3.305608096359798E-4 42-43 0.0 0.0 0.0 0.4098623478676514 3.305608096359798E-4 44-45 0.0 0.0 0.0 0.5295253609558761 3.470888501177788E-4 46-47 0.0 0.0 0.0 0.6675344989788976 3.636168905995778E-4 48-49 0.0 3.3056080963597985E-5 0.0 0.8126672224495746 3.636168905995778E-4 50-51 0.0 3.3056080963597985E-5 0.0 0.9747246593736136 3.636168905995778E-4 52-53 0.0 3.3056080963597985E-5 0.0 1.1326666142176849 3.8014493108137683E-4 54-55 0.0 3.3056080963597985E-5 0.0 1.3065416000862102 4.297290525267738E-4 56-57 0.0 3.3056080963597985E-5 0.0 1.5039359875603355 4.462570930085728E-4 58-59 0.0 3.3056080963597985E-5 0.0 1.7171477097755425 4.6278513349037176E-4 60-61 0.0 3.3056080963597985E-5 0.0 1.9483419400349469 4.6278513349037176E-4 62-63 0.0 3.3056080963597985E-5 0.0 2.184593750681782 4.958412144539698E-4 64-65 0.0 3.3056080963597985E-5 0.0 2.431010306224923 4.958412144539698E-4 66-67 0.0 3.3056080963597985E-5 0.0 2.7081690170642103 5.123692549357688E-4 68-69 0.0 3.3056080963597985E-5 0.0 3.0110453588931767 5.619533763811657E-4 70-71 0.0 3.3056080963597985E-5 0.0 3.3390443222544777 5.950094573447637E-4 72-73 0.0 3.3056080963597985E-5 0.0 3.6784806896291835 6.776496597537586E-4 74-75 0.0 3.3056080963597985E-5 0.0 4.026214133325753 7.437618216809545E-4 76-77 0.0 3.3056080963597985E-5 0.0 4.393500248912289 7.933459431263516E-4 78-79 0.0 3.3056080963597985E-5 0.0 4.790074052232574 7.933459431263516E-4 80-81 0.0 3.3056080963597985E-5 0.0 5.208415284867389 7.933459431263516E-4 82-83 0.0 3.3056080963597985E-5 0.0 5.641912730624012 7.933459431263516E-4 84-85 3.3056080963597985E-5 3.3056080963597985E-5 0.0 6.069807170657307 7.933459431263516E-4 86-87 3.3056080963597985E-5 3.3056080963597985E-5 0.0 6.511403356250012 8.098739836081507E-4 88-89 3.3056080963597985E-5 3.3056080963597985E-5 0.0 6.971097746170288 8.429300645717485E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6610 0.0 56.631493 1 GTATCAA 12740 0.0 46.37583 1 TATCAAC 17170 0.0 34.240128 2 ATCAACG 17485 0.0 33.541737 3 TCAACGC 17850 0.0 32.882496 4 CAACGCA 18020 0.0 32.598118 5 AACGCAG 18665 0.0 31.513178 6 ACGCAGA 21365 0.0 27.369604 7 CGCAGAG 21755 0.0 26.74794 8 GTACATG 15335 0.0 26.276213 1 TACATGG 15355 0.0 25.906673 2 TGGTATC 2835 0.0 25.649233 2 GTGGTAT 2980 0.0 25.283117 1 GCAGAGT 22875 0.0 24.503439 9 GTAAGGT 1460 0.0 24.088785 4 ACATGGG 16010 0.0 23.510933 3 GAGTACT 12905 0.0 22.931507 12-13 TAAGGTA 685 0.0 22.895988 4 AGTACTT 13215 0.0 22.753021 12-13 TAAGGTG 1705 0.0 22.578207 5 >>END_MODULE