FastQCFastQC Report
Thu 26 May 2016
SRR938624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025162
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138000.45617391729765217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111640.3690380878776079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80870.2673245267526169No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG37010.12234055564627613No Hit
ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT32560.10763059961747504No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC30430.10058965437222867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71650.056.366121
GTATCAA130250.043.5918851
TATCAAC178200.031.9086592
ATCAACG179750.031.5510963
TCAACGC183300.030.9654434
CAACGCA185800.030.5487945
AACGCAG192050.029.5793556
TGGTATC29800.029.490192
GTGGTAT33500.027.2315181
ACGCAGA220550.025.4986087
CGCAGAG224450.025.034398
GTACATG149950.023.4160391
GCAGAGT235600.023.3859259
TACATGG152250.022.6517312
GAGTACT136550.022.07047812-13
AGTACTT139550.021.91936112-13
ACATGGG155650.021.1782173
GTACTTT141700.021.05046314-15
AGAGTAC223300.020.8240810-11
CAGAGTA231000.020.19162810-11
Produced by FastQC (version 0.11.5)