##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025162 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827631379740986 31.0 31.0 34.0 30.0 34.0 2 32.07469219830211 33.0 31.0 34.0 30.0 34.0 3 32.12405385232262 33.0 31.0 34.0 30.0 34.0 4 35.785788331335645 37.0 35.0 37.0 35.0 37.0 5 35.68147457888205 37.0 35.0 37.0 33.0 37.0 6 35.71829244185931 37.0 35.0 37.0 33.0 37.0 7 35.63985366734079 37.0 35.0 37.0 33.0 37.0 8 35.612681568788716 37.0 35.0 37.0 33.0 37.0 9 37.14554460223948 39.0 37.0 39.0 33.0 39.0 10-11 37.0753782772625 39.0 37.0 39.0 33.0 39.0 12-13 36.9592491245097 39.0 37.0 39.0 33.0 39.0 14-15 37.9493969248589 40.0 37.0 41.0 33.0 41.0 16-17 38.047106072335964 40.0 37.0 41.0 33.0 41.0 18-19 38.208577094383706 40.0 37.5 41.0 33.0 41.0 20-21 38.16760143754284 40.0 37.0 41.0 33.0 41.0 22-23 38.046160998981215 40.0 37.0 41.0 33.0 41.0 24-25 37.923777470429684 40.0 37.0 41.0 33.0 41.0 26-27 37.70163399513811 40.0 37.0 41.0 32.0 41.0 28-29 37.55143427029692 39.5 36.5 41.0 32.0 41.0 30-31 37.264266508702676 39.0 36.0 41.0 31.0 41.0 32-33 37.130429378658064 39.0 36.0 41.0 31.0 41.0 34-35 36.978463136850195 39.0 35.5 41.0 31.0 41.0 36-37 36.8388291271674 39.0 35.0 41.0 30.5 41.0 38-39 36.61263181938686 39.0 35.0 40.5 30.0 41.0 40-41 36.36527399193828 39.0 35.0 40.0 30.0 41.0 42-43 36.06358915654765 38.5 35.0 40.0 29.5 41.0 44-45 35.708195627209385 38.0 35.0 40.0 28.5 41.0 46-47 35.20680462732244 38.0 34.0 40.0 26.5 41.0 48-49 34.735254178123355 38.0 34.0 40.0 25.5 41.0 50-51 34.312433681237565 38.0 33.0 40.0 24.0 41.0 52-53 33.72035150514253 37.0 33.0 40.0 23.0 41.0 54-55 33.290060168678565 37.0 32.0 40.0 21.5 41.0 56-57 32.89583400822832 36.5 31.5 39.0 20.0 40.5 58-59 32.37366792257737 36.0 31.0 39.0 17.5 40.0 60-61 31.892336840142775 35.0 31.0 39.0 14.5 40.0 62-63 31.401747245271494 35.0 30.0 38.5 10.5 40.0 64-65 30.765144478213067 34.5 29.0 38.0 7.5 40.0 66-67 30.137019108398164 34.0 28.0 37.5 6.0 40.0 68-69 29.439248542722673 34.0 27.5 37.0 2.0 39.0 70-71 28.686812970677273 33.5 26.0 36.0 2.0 39.0 72-73 28.1316379089781 33.0 26.0 36.0 2.0 38.5 74-75 27.438218515239846 32.0 25.0 35.0 2.0 37.5 76-77 26.308409268660654 30.5 24.0 34.0 2.0 36.0 78-79 26.806608373369755 32.0 25.0 35.0 2.0 36.0 80-81 26.456634719066287 32.0 24.0 35.0 2.0 36.0 82-83 25.80707330714851 31.5 22.0 34.5 2.0 36.0 84-85 25.063642376838 31.0 19.5 34.0 2.0 35.0 86-87 24.190536903478225 30.5 15.5 34.0 2.0 35.0 88-89 23.27098019874638 30.0 7.0 34.0 2.0 35.0 90-91 22.456133093037664 29.0 2.0 34.0 2.0 35.0 92-93 21.412318579963653 29.0 2.0 34.0 2.0 35.0 94-95 20.267559720768674 27.5 2.0 33.0 2.0 34.5 96-97 18.92299453715206 25.5 2.0 33.0 2.0 34.0 98-99 17.1861483781695 21.0 2.0 32.0 2.0 34.0 100-101 12.904982609195805 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32059093581752407 1101 2 0.3204867266412208 1101 3 0.31405027772135696 1101 4 0.1663787363165099 1101 5 0.15606160374969136 1101 6 0.22749668786207167 1101 7 0.3027735757250696 1101 8 0.3195269682678159 1101 9 0.3670463740246106 1101 10-11 0.41590064588326925 1101 12-13 0.363965106887143 1101 14-15 0.5956133196061657 1101 16-17 0.48944219210086715 1101 18-19 0.5381866558575581 1101 20-21 0.5589867663537262 1101 22-23 0.4364216711710256 1101 24-25 0.4488777272578872 1101 26-27 0.5904753459725711 1101 28-29 0.6058768951095104 1101 30-31 0.6308809971214586 1101 32-33 0.6074253264194525 1101 34-35 0.5211468559876806 1101 36-37 0.613633210410633 1101 38-39 0.7634352676637377 1101 40-41 0.7561048909675847 1101 42-43 0.7436249060244933 1101 44-45 0.7992055101473596 1101 46-47 0.8451165228727717 1101 48-49 0.7503018210746717 1101 50-51 0.9680598640301099 1101 52-53 0.939385689214717 1101 54-55 0.9947697305573371 1101 56-57 1.1249009316197203 1101 58-59 0.9848692865237183 1101 60-61 1.041784653256986 1101 62-63 1.1359456843951214 1101 64-65 1.1638069262002837 1101 66-67 1.3075108411343983 1101 68-69 1.477561384590043 1101 70-71 1.5583310651436406 1101 72-73 1.61786545983529 1101 74-75 1.510592775246696 1101 76-77 1.4740175766079666 1101 78-79 1.427229854885514 1101 80-81 1.6391462929966316 1101 82-83 1.8512209649563829 1101 84-85 1.9660536698607558 1101 86-87 1.9693853754996802 1101 88-89 1.8166291008631603 1101 90-91 2.2071192403561035 1101 92-93 2.715302527715437 1101 94-95 2.5435700572051942 1101 96-97 2.449266714008157 1101 98-99 2.519684288135281 1101 100-101 2.3608738125812287 1102 1 0.31111720493145967 1102 2 0.32896903076961337 1102 3 0.23228268468757562 1102 4 0.19614746770611902 1102 5 0.19982329356677297 1102 6 0.2480866738380456 1102 7 0.2773965579868616 1102 8 0.3479301235977701 1102 9 0.41703570193872963 1102 10-11 0.39414318131033355 1102 12-13 0.3683105998849001 1102 14-15 0.5366341371881518 1102 16-17 0.5026147652409776 1102 18-19 0.44932337830466196 1102 20-21 0.3881217862774591 1102 22-23 0.4380260539411651 1102 24-25 0.3926700304292936 1102 26-27 0.4515021785024871 1102 28-29 0.4993412320548174 1102 30-31 0.5010992888762402 1102 32-33 0.4393807456595695 1102 34-35 0.474456041847823 1102 36-37 0.47878326344538635 1102 38-39 0.608381321021163 1102 40-41 0.7061707397416228 1102 42-43 0.7509964113547696 1102 44-45 0.5719517710342643 1102 46-47 0.6830590824999163 1102 48-49 0.6092242986419905 1102 50-51 0.7239206178601592 1102 52-53 0.6869963068879912 1102 54-55 0.6049438609201161 1102 56-57 0.832956878401582 1102 58-59 1.0347887197908676 1102 60-61 1.0116906236627408 1102 62-63 1.0124739721867009 1102 64-65 1.0810311216728117 1102 66-67 1.2581164319044973 1102 68-69 1.3513066629435961 1102 70-71 1.6093840943876607 1102 72-73 1.6932819718068153 1102 74-75 1.6263512123322457 1102 76-77 1.565477477873639 1102 78-79 1.5025096464140866 1102 80-81 1.8679651461131535 1102 82-83 2.007601970447247 1102 84-85 2.0293273647723744 1102 86-87 2.178651890051544 1102 88-89 2.018086329096988 1102 90-91 2.2417093245353144 1102 92-93 2.4853043590052906 1102 94-95 2.468146969124412 1102 96-97 2.607295692479102 1102 98-99 2.4014551754866567 1102 100-101 2.2483692969862066 1103 1 0.24822622874508582 1103 2 0.22718746059621964 1103 3 0.19349174242621814 1103 4 0.08757184555843622 1103 5 0.177001238733709 1103 6 0.1889211762091776 1103 7 0.3010847141982964 1103 8 0.2007965222423138 1103 9 0.2944391061188867 1103 10-11 0.29370672846780366 1103 12-13 0.3484562603056034 1103 14-15 0.44299252050418403 1103 16-17 0.3144657321040043 1103 18-19 0.3699179637172847 1103 20-21 0.3465837861548806 1103 22-23 0.3773303714909204 1103 24-25 0.43446765587103897 1103 26-27 0.5243722616067217 1103 28-29 0.516689017059953 1103 30-31 0.3742485536491884 1103 32-33 0.35868100475406095 1103 34-35 0.5343167490034872 1103 36-37 0.48470723279802286 1103 38-39 0.4046705730637825 1103 40-41 0.540165743643243 1103 42-43 0.4286426231173408 1103 44-45 0.3520034620492467 1103 46-47 0.5025896263570502 1103 48-49 0.4296630485412578 1103 50-51 0.5126531685534559 1103 52-53 0.5655013693404314 1103 54-55 0.4247133396957494 1103 56-57 0.7004573621829664 1103 58-59 0.6783249662360973 1103 60-61 0.5318945887880169 1103 62-63 0.7090112436211093 1103 64-65 0.7816358363334892 1103 66-67 0.8391561468571282 1103 68-69 0.8288938919837996 1103 70-71 1.0403385986697877 1103 72-73 1.1105650676408914 1103 74-75 1.0781059030813296 1103 76-77 0.9696879201197923 1103 78-79 1.1870561972820681 1103 80-81 1.1960735802201974 1103 82-83 1.475991502045531 1103 84-85 1.4754910628792821 1103 86-87 1.3544086399479092 1103 88-89 1.2386606584263973 1103 90-91 1.5028641649791474 1103 92-93 1.796028513377152 1103 94-95 1.52292718760539 1103 96-97 1.6256804103923308 1103 98-99 1.7515499652697706 1103 100-101 1.89409938944274 1104 1 0.1566177430134701 1104 2 0.12741878299889464 1104 3 0.18229833287142583 1104 4 0.15388788401340037 1104 5 0.1182066534567241 1104 6 0.10426264656710771 1104 7 0.15575957055272482 1104 8 0.07829001308598293 1104 9 0.23153690670832816 1104 10-11 0.1251817639222068 1104 12-13 0.16884451216980523 1104 14-15 0.2633872140005806 1104 16-17 0.284003253424963 1104 18-19 0.29483797164162695 1104 20-21 0.24604154763797226 1104 22-23 0.2936452628833095 1104 24-25 0.2007325229038841 1104 26-27 0.23011369968867967 1104 28-29 0.31697728575151274 1104 30-31 0.35171945357571843 1104 32-33 0.48545814654308117 1104 34-35 0.32998037781900535 1104 36-37 0.3594624649007656 1104 38-39 0.35564845238579323 1104 40-41 0.3899306071162414 1104 42-43 0.406323610662497 1104 44-45 0.341999776757568 1104 46-47 0.4979158266563246 1104 48-49 0.6362854080794875 1104 50-51 0.5017164071868834 1104 52-53 0.7116012605220519 1104 54-55 0.6670253364220429 1104 56-57 0.8029012383323035 1104 58-59 0.7511324747834109 1104 60-61 0.8511414286306156 1104 62-63 0.9388679489477418 1104 64-65 1.0724100967917316 1104 66-67 1.0908014470818301 1104 68-69 1.316483070448541 1104 70-71 1.3489956211959502 1104 72-73 1.4348888177466996 1104 74-75 1.3864796633072807 1104 76-77 1.426659229655023 1104 78-79 1.411342811943289 1104 80-81 1.464853113549939 1104 82-83 1.484683354590011 1104 84-85 1.810230536715462 1104 86-87 1.812473670824236 1104 88-89 1.734669784750082 1104 90-91 1.8220161320106492 1104 92-93 2.1238666258930294 1104 94-95 2.162873624868581 1104 96-97 2.3492379905039193 1104 98-99 2.5856347093488097 1104 100-101 2.2185814680873825 1105 1 0.10980458997204678 1105 2 0.11263928837485082 1105 3 0.10359328034502369 1105 4 0.0973899794613402 1105 5 0.0807390813486677 1105 6 0.08996193683953635 1105 7 0.09961327533449804 1105 8 0.14025968375167253 1105 9 0.1534090768241967 1105 10-11 0.23134194014936327 1105 12-13 0.2203839101618712 1105 14-15 0.37075271646040164 1105 16-17 0.26552129857572027 1105 18-19 0.29832114516732133 1105 20-21 0.23067083677054256 1105 22-23 0.28248147888059805 1105 24-25 0.23245639194478684 1105 26-27 0.301981510878214 1105 28-29 0.2521546897637208 1105 30-31 0.3161817116539609 1105 32-33 0.3640002835278935 1105 34-35 0.4152179757621539 1105 36-37 0.48080039042284994 1105 38-39 0.4136443156413918 1105 40-41 0.4676707445494799 1105 42-43 0.5550655108605724 1105 44-45 0.5440309720866807 1105 46-47 0.5895719838247402 1105 48-49 0.6804550036622743 1105 50-51 0.731947228688469 1105 52-53 0.8132019354747158 1105 54-55 0.8512131890969812 1105 56-57 0.8891398344856611 1105 58-59 1.0505082529429046 1105 60-61 0.9886817996336923 1105 62-63 0.788270154103369 1105 64-65 0.8647824260915122 1105 66-67 0.9880323332435097 1105 68-69 1.2645564089282288 1105 70-71 1.3968897665198732 1105 72-73 1.5186372499004115 1105 74-75 1.5539435541867874 1105 76-77 1.4606169814184042 1105 78-79 1.6324789722557007 1105 80-81 1.6668914933332708 1105 82-83 1.811518706522648 1105 84-85 1.946533465903233 1105 86-87 2.0909662779672367 1105 88-89 1.9999115239413108 1105 90-91 1.9720478025860508 1105 92-93 2.214774044978654 1105 94-95 2.173232267461394 1105 96-97 2.4404278015323975 1105 98-99 2.370664311980935 1105 100-101 2.4360245508573364 1106 1 0.09206201130612612 1106 2 0.06843209491247393 1106 3 0.15026617423328759 1106 4 0.1684798426049099 1106 5 0.11965018334801414 1106 6 0.04267118472221654 1106 7 0.0955078742238129 1106 8 0.08010178386930988 1106 9 0.00810618558538323 1106 10-11 0.14184906334183012 1106 12-13 0.11804123940088829 1106 14-15 0.13341401570952627 1106 16-17 0.11628227103120992 1106 18-19 0.14591919003375153 1106 20-21 0.06936382272468222 1106 22-23 0.23603699867467753 1106 24-25 0.13523518014637403 1106 26-27 -0.08847257948291798 1106 28-29 0.24099936783435538 1106 30-31 0.35271513301055535 1106 32-33 0.19640722905485575 1106 34-35 0.1474006141543711 1106 36-37 0.18484061832999288 1106 38-39 0.29554120502538694 1106 40-41 0.3617391700553583 1106 42-43 0.31974162411539453 1106 44-45 0.37282936707277514 1106 46-47 0.0027495615745039004 1106 48-49 0.24494270559445397 1106 50-51 0.42453133526527154 1106 52-53 0.37407467689958906 1106 54-55 0.4008225772067959 1106 56-57 0.42265801804916947 1106 58-59 0.7723915344371548 1106 60-61 0.693202931364862 1106 62-63 0.7795146089612608 1106 64-65 0.8618775647938044 1106 66-67 0.7644309201742239 1106 68-69 0.7889146462145575 1106 70-71 0.7902991640935078 1106 72-73 0.9848419264868582 1106 74-75 0.8467650483989537 1106 76-77 0.9162296835329862 1106 78-79 1.2500710574167435 1106 80-81 1.215239003983715 1106 82-83 1.452410394349151 1106 84-85 1.3710734127098902 1106 86-87 1.3422774548433267 1106 88-89 1.5069738998980355 1106 90-91 1.5413288044669322 1106 92-93 1.4179390779550616 1106 94-95 1.3219404664025731 1106 96-97 1.3538954432921955 1106 98-99 1.4360657207560656 1106 100-101 1.4839218525637214 1107 1 -0.0302481783121884 1107 2 0.04599922087349739 1107 3 0.02097370455392422 1107 4 -0.01321645761630208 1107 5 -0.027812490570418902 1107 6 0.0668581925291889 1107 7 0.017736525035751072 1107 8 0.0464057434317624 1107 9 0.07225255467302816 1107 10-11 0.037184956494705546 1107 12-13 0.12202194887958484 1107 14-15 0.12062858038576252 1107 16-17 -0.03724146076116597 1107 18-19 -0.02838743310331182 1107 20-21 0.05099511762746545 1107 22-23 0.06778430520119372 1107 24-25 0.09424351540035758 1107 26-27 0.14795293190676517 1107 28-29 0.04862936461082512 1107 30-31 -0.03503080014742466 1107 32-33 0.07973763190170047 1107 34-35 0.09077440800177783 1107 36-37 0.10314281326436969 1107 38-39 0.012832025712086192 1107 40-41 -0.06484907441691234 1107 42-43 -0.11257443439612302 1107 44-45 -0.005958286708462879 1107 46-47 -0.018384790142484064 1107 48-49 0.06943200892277446 1107 50-51 0.18121096339444875 1107 52-53 0.09945166022512097 1107 54-55 0.134534616516504 1107 56-57 0.21358305382883458 1107 58-59 0.28335363295060745 1107 60-61 0.3503063117998231 1107 62-63 0.5620611264740845 1107 64-65 0.6853875312199342 1107 66-67 0.5076406067784944 1107 68-69 0.7816054585058119 1107 70-71 0.694164257960157 1107 72-73 1.0665565620491826 1107 74-75 0.8993704488895347 1107 76-77 0.9545064901581028 1107 78-79 0.9989916285606384 1107 80-81 1.1465742405492172 1107 82-83 1.2968736361483444 1107 84-85 1.2934464097817795 1107 86-87 1.5064177649521575 1107 88-89 1.602165803201487 1107 90-91 1.6901207906160316 1107 92-93 1.897557247446482 1107 94-95 1.7149294952947933 1107 96-97 1.9304261868674963 1107 98-99 2.0942243733342174 1107 100-101 1.9867146259840514 1108 1 -0.03159459378792562 1108 2 -0.009687983362155705 1108 3 -0.019710704880843366 1108 4 0.02984568818344968 1108 5 -0.017837150798733603 1108 6 -0.05627996585635486 1108 7 0.0060395054444057905 1108 8 -0.059110736146323006 1108 9 -0.0044636986217838626 1108 10-11 -3.620968179447459E-4 1108 12-13 -0.0026068636993059613 1108 14-15 5.65921362344568E-4 1108 16-17 -0.12826991562054246 1108 18-19 -0.2652482606417905 1108 20-21 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-0.052945414776941035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 14.0 4 57.0 5 123.0 6 275.0 7 686.0 8 1668.0 9 2894.0 10 4837.0 11 7658.0 12 10816.0 13 15125.0 14 20453.0 15 27323.0 16 35257.0 17 44829.0 18 42386.0 19 35226.0 20 35747.0 21 38688.0 22 41918.0 23 47437.0 24 53438.0 25 61128.0 26 70699.0 27 82596.0 28 97413.0 29 114567.0 30 134155.0 31 156581.0 32 185428.0 33 222442.0 34 271971.0 35 330866.0 36 383774.0 37 337302.0 38 107401.0 39 1976.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.52713258264097 19.254743407635477 15.629372756585752 22.588751253137804 2 16.213761737525733 20.840205672626386 38.71744544362665 24.22858714622123 3 19.148011991618162 24.534653746818712 29.9404352829134 26.37689897864972 4 13.64079014611449 17.391531428730097 35.72658257640417 33.24109584875124 5 14.140422788952614 37.252334594978755 33.259078062244846 15.348164553823777 6 32.28349423931677 35.861418330654686 16.97895848222343 14.876128947805109 7 29.099664745226868 32.87919787436177 19.77266010878095 18.24847727163041 8 27.172984957154316 34.113215206209 19.396368918129593 19.317430918507096 9 27.262852490436025 16.43606125045576 19.55187059960273 36.74921565950549 10-11 25.474883659123048 26.53514092798997 26.458335123871052 21.531640289015925 12-13 26.537058180685865 23.810278590039143 27.116977537070742 22.53568569220425 14-15 23.185964916920152 25.10381262226618 25.90385242178766 25.80637003902601 16-17 22.446797890493137 28.31140943856891 26.46845028464591 22.77334238629204 18-19 22.513389365594307 26.386537316018117 29.416077552210428 21.68399576617715 20-21 23.19935262971041 25.465842820979507 29.56061526622376 21.774189283086326 22-23 23.04149331506875 25.237342661318635 29.443960356503222 22.277203667109397 24-25 22.869499406055933 25.570221841043146 29.414734936654508 22.145543816246416 26-27 22.177249194560016 26.120785169887462 29.411919305087658 22.290046330464865 28-29 22.31827421763939 26.197132676320273 29.467741410317583 22.01685169572275 30-31 22.389627398466594 25.864284292874235 29.23952502378385 22.506563284875323 32-33 22.341186000498155 25.912975238752583 29.035679381901826 22.710159378847433 34-35 23.035455182899316 26.05861889190941 28.733981495348047 22.171944429843222 36-37 23.10541775780579 25.544985564237656 28.942182904117054 22.4074137738395 38-39 22.491201854016072 25.97227571546407 28.793765507300517 22.742756923219336 40-41 22.82378300106701 25.761193067658024 28.78383169838081 22.631192232894158 42-43 22.787428179835164 25.80320981446798 28.526958729138663 22.88240327655819 44-45 22.536723944494433 26.135402748568826 28.59150557759679 22.73636772933995 46-47 22.908658474806685 26.435879784771537 27.869838181978956 22.785623558442825 48-49 22.845586451237985 26.198745720063915 27.986121073846626 22.969546754851475 50-51 23.16232981903118 26.55889172216232 27.501766847527502 22.777011611279 52-53 23.298140727670123 26.542380209721 27.26305235884888 22.89642670376 54-55 22.734137874269216 26.597583864930208 27.57944863779196 23.088829623008618 56-57 22.749938680969812 26.656638553571675 27.644188972359167 22.949233793099346 58-59 22.9084095331093 26.226843388882976 27.46366971421696 23.401077363790765 60-61 22.740930898907234 26.543966901607252 27.70641704477314 23.008685154712374 62-63 22.535206379030278 26.708470488522597 27.623975178849925 23.132347953597197 64-65 22.297393673752964 26.43271418671121 27.564010282436442 23.70588185709939 66-67 22.433718367791514 26.505347437581822 27.496656766850997 23.564277427775668 68-69 22.57594261667145 26.39913431805151 27.576488874277942 23.448434190999095 70-71 22.548842012427762 26.58989832610617 27.21174932119338 23.649510340272688 72-73 22.670015696718963 26.662820031175265 27.021892600857246 23.645271671248526 74-75 22.491407071753514 26.93978041506537 26.83912464853122 23.729687864649893 76-77 22.521736026037615 26.53054613273603 27.216393700568766 23.73132414065759 78-79 22.729939751986837 26.62715252935215 27.258755068323616 23.384152650337402 80-81 22.649279806211776 26.99847412928515 27.0152005283691 23.337045536133978 82-83 22.680168025975764 26.82488589323921 26.89089963976725 23.604046441017775 84-85 22.673979335720897 26.556304449767165 26.938290635784686 23.831425578727252 86-87 22.771076849638533 26.616087670993778 26.71802030851193 23.89481517085576 88-89 22.58305505622509 26.72437046346609 26.65035789818859 24.04221658212023 90-91 22.503791575100294 26.557322533129174 26.95337189037951 23.985514001391014 92-93 22.53260959931005 26.15718026031484 26.93517615439527 24.375033985979844 94-95 22.372192276142794 26.30461302949481 26.86691395941272 24.45628073494968 96-97 22.476283914712667 26.21472833520982 26.77995426360638 24.52903348647114 98-99 22.36743024009954 26.31718565815649 26.450748753289904 24.864635348454065 100-101 22.662764504560997 26.021379692085763 26.58296973003581 24.73288607331743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1110.0 1 788.5 2 573.0 3 660.0 4 571.0 5 593.0 6 1043.5 7 2272.0 8 3632.0 9 4155.0 10 3569.0 11 2971.0 12 3047.0 13 3394.5 14 3857.5 15 4237.5 16 4576.0 17 4973.5 18 5473.0 19 5887.5 20 6507.5 21 7386.0 22 8354.5 23 9590.0 24 11232.5 25 13340.0 26 16162.0 27 20100.0 28 23805.0 29 27387.5 30 31809.5 31 36755.5 32 42878.5 33 46403.0 34 47614.0 35 49921.5 36 54324.5 37 60015.0 38 64560.5 39 68503.5 40 73024.0 41 81435.0 42 90786.0 43 97892.5 44 101903.5 45 105848.5 46 108338.5 47 112174.0 48 117509.0 49 118765.5 50 119881.0 51 117321.5 52 113861.5 53 113562.0 54 113744.0 55 107300.5 56 95139.5 57 85877.0 58 79090.0 59 70374.5 60 58768.5 61 48752.5 62 41060.5 63 32845.5 64 26008.0 65 20642.5 66 15710.5 67 12599.0 68 9820.5 69 7112.0 70 5561.0 71 4511.5 72 3766.5 73 3120.0 74 2461.0 75 1969.0 76 1334.5 77 750.5 78 445.0 79 267.0 80 143.0 81 86.5 82 54.0 83 23.0 84 18.5 85 12.0 86 4.0 87 2.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024924285046552877 2 0.011734908742077284 3 0.001619747967216301 4 0.0 5 3.9667297156317576E-4 6 0.0 7 0.0 8 9.916824289079394E-5 9 2.9750472867238185E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015040516838437082 26-27 0.00867722125294447 28-29 2.1486452626338687E-4 30-31 0.0 32-33 3.801449310813768E-4 34-35 0.009949880370042992 36-37 0.0011900189146895274 38-39 0.012462142523276439 40-41 0.011718380701595484 42-43 0.00523938883273028 44-45 7.933459431263515E-4 46-47 1.4875236433619092E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1569628337259293E-4 66-67 7.107057407173567E-4 68-69 0.0010743226313169344 70-71 0.0 72-73 2.4792060722698484E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.322243238543919E-4 82-83 0.0011404347932441304 84-85 0.00606579085682023 86-87 0.004132010120449748 88-89 0.0 90-91 0.0011239067527623315 92-93 5.784814168629647E-4 94-95 8.264020240899496E-5 96-97 0.0 98-99 0.0 100-101 4.958412144539697E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025162.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.63384135389639 #Duplication Level Percentage of deduplicated Percentage of total 1 67.41815216837523 27.394584995826047 2 15.30714194379181 12.439759546512194 3 6.526961809330454 7.9564659204982275 4 3.36209493180714 5.4645932830316175 5 1.9576779747619781 3.9773988124247697 6 1.2289606060161942 2.996243417703024 7 0.8178700373365057 2.3263240941665817 8 0.5910547250062843 1.9213459141900973 9 0.43179444210514384 1.5790920172195153 >10 2.1013343929515758 15.057818299329442 >50 0.1438710461303316 4.034679849041491 >100 0.09790760269657155 7.850083383743551 >500 0.010200483663280177 2.849467184218922 >1k 0.004733024419685513 3.056212219832145 >5k 8.160386930492264E-5 0.26815510878693005 >10k+ 1.6320773860984528E-4 0.8277759534755683 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13800 0.45617391729765217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11164 0.3690380878776079 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8087 0.2673245267526169 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3701 0.12234055564627613 No Hit ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT 3256 0.10763059961747504 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3043 0.10058965437222867 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3056080963597985E-5 2 0.0 0.0 0.0 0.0 3.3056080963597985E-5 3 0.0 0.0 0.0 0.0 3.3056080963597985E-5 4 0.0 0.0 0.0 3.3056080963597985E-5 3.3056080963597985E-5 5 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 6 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 7 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 8 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 9 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 10-11 0.0 0.0 0.0 3.3056080963597985E-5 6.611216192719597E-5 12-13 0.0 0.0 0.0 3.3056080963597985E-5 9.916824289079395E-5 14-15 0.0 0.0 0.0 3.3056080963597985E-5 1.1569628337259295E-4 16-17 0.0 0.0 0.0 8.264020240899496E-5 1.652804048179899E-4 18-19 0.0 0.0 0.0 1.983364857815879E-4 1.983364857815879E-4 20-21 0.0 0.0 0.0 2.313925667451859E-4 2.644486477087839E-4 22-23 0.0 0.0 0.0 5.784814168629648E-4 2.644486477087839E-4 24-25 0.0 0.0 0.0 0.0012561310766167233 2.644486477087839E-4 26-27 0.0 0.0 0.0 0.002264341546006462 2.644486477087839E-4 28-29 0.0 0.0 0.0 0.006214543221156421 2.8097668819058286E-4 30-31 0.0 0.0 0.0 0.019585727970931804 2.9750472867238185E-4 32-33 0.0 0.0 0.0 0.04384889139821273 2.9750472867238185E-4 34-35 0.0 0.0 0.0 0.07954945883889855 3.470888501177788E-4 36-37 0.0 0.0 0.0 0.1360918853271329 3.636168905995778E-4 38-39 0.0 0.0 0.0 0.22030225158189876 3.636168905995778E-4 40-41 0.0 0.0 0.0 0.31555334887850633 3.636168905995778E-4 42-43 0.0 0.0 0.0 0.4132010120449748 3.636168905995778E-4 44-45 0.0 0.0 0.0 0.5333433383071717 3.636168905995778E-4 46-47 0.0 0.0 0.0 0.6721292942328378 3.8014493108137683E-4 48-49 0.0 0.0 0.0 0.8175595224321871 3.966729715631758E-4 50-51 0.0 0.0 0.0 0.9795673752347809 4.297290525267738E-4 52-53 0.0 0.0 0.0 1.138170451698124 4.297290525267738E-4 54-55 0.0 0.0 0.0 1.3122768301333945 4.6278513349037176E-4 56-57 0.0 0.0 0.0 1.5105637317935372 4.6278513349037176E-4 58-59 0.0 0.0 0.0 1.7244365756280158 4.6278513349037176E-4 60-61 0.0 0.0 0.0 1.9558456704136837 4.6278513349037176E-4 62-63 0.0 0.0 0.0 2.1920148408581093 5.619533763811657E-4 64-65 0.0 0.0 0.0 2.439390022749195 5.950094573447637E-4 66-67 0.0 0.0 0.0 2.7190279396607524 5.950094573447637E-4 68-69 0.0 0.0 0.0 3.024482655804879 6.115374978265626E-4 70-71 0.0 0.0 0.0 3.357820176241801 6.941777002355576E-4 72-73 0.0 0.0 0.0 3.7042809608212717 7.602898621627537E-4 74-75 0.0 0.0 0.0 4.062741102790528 8.759861455353466E-4 76-77 0.0 0.0 0.0 4.443679379815031 8.925141860171455E-4 78-79 0.0 0.0 0.0 4.856202742200252 9.090422264989445E-4 80-81 0.0 0.0 0.0 5.294675128141898 9.255702669807435E-4 82-83 0.0 0.0 0.0 5.754633966709882 9.916824289079396E-4 84-85 0.0 0.0 0.0 6.208146869489965 9.916824289079396E-4 86-87 0.0 0.0 0.0 6.683113168815423 0.0010412665503533366 88-89 0.0 0.0 0.0 7.187648132562818 0.0010908506717987334 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7165 0.0 56.36612 1 GTATCAA 13025 0.0 43.591885 1 TATCAAC 17820 0.0 31.908659 2 ATCAACG 17975 0.0 31.551096 3 TCAACGC 18330 0.0 30.965443 4 CAACGCA 18580 0.0 30.548794 5 AACGCAG 19205 0.0 29.579355 6 TGGTATC 2980 0.0 29.49019 2 GTGGTAT 3350 0.0 27.231518 1 ACGCAGA 22055 0.0 25.498608 7 CGCAGAG 22445 0.0 25.03439 8 GTACATG 14995 0.0 23.416039 1 GCAGAGT 23560 0.0 23.385925 9 TACATGG 15225 0.0 22.651731 2 GAGTACT 13655 0.0 22.070478 12-13 AGTACTT 13955 0.0 21.919361 12-13 ACATGGG 15565 0.0 21.178217 3 GTACTTT 14170 0.0 21.050463 14-15 AGAGTAC 22330 0.0 20.82408 10-11 CAGAGTA 23100 0.0 20.191628 10-11 >>END_MODULE