##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938623_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3469110 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.117797936646575 31.0 31.0 34.0 28.0 34.0 2 31.148256469238508 31.0 31.0 34.0 27.0 34.0 3 31.18880202703287 31.0 31.0 34.0 27.0 34.0 4 34.853499600762156 37.0 35.0 37.0 32.0 37.0 5 34.82305576934718 37.0 35.0 37.0 32.0 37.0 6 34.848803583628076 37.0 35.0 37.0 32.0 37.0 7 34.759155230015764 37.0 35.0 37.0 31.0 37.0 8 34.712959231618484 37.0 35.0 37.0 31.0 37.0 9 36.20535958790583 39.0 35.0 39.0 32.0 39.0 10-11 36.03611127926183 39.0 35.0 39.0 30.0 39.0 12-13 35.894192890972036 38.0 35.0 39.0 30.0 39.0 14-15 36.84300670777231 39.0 36.0 41.0 30.5 41.0 16-17 36.66203262508252 39.0 36.0 40.5 30.0 41.0 18-19 36.80878020587413 39.0 36.0 41.0 30.0 41.0 20-21 36.77166319315329 39.0 36.0 41.0 30.0 41.0 22-23 36.692121033925126 39.0 36.0 41.0 30.0 41.0 24-25 36.53020414457887 39.0 36.0 41.0 30.0 41.0 26-27 36.300022484153 39.0 35.5 41.0 29.0 41.0 28-29 36.081360925424676 39.0 35.0 40.0 28.5 41.0 30-31 35.79669295583017 38.0 35.0 40.0 27.0 41.0 32-33 35.49204133048534 38.0 34.0 40.0 27.0 41.0 34-35 35.22102599802255 38.0 34.0 40.0 26.0 41.0 36-37 34.90407972073529 38.0 34.0 40.0 25.0 41.0 38-39 34.72345183634996 38.0 33.0 40.0 24.5 41.0 40-41 34.59203988919348 38.0 33.0 40.0 24.0 41.0 42-43 34.250751921962696 38.0 33.0 40.0 23.5 41.0 44-45 33.870520392838515 38.0 33.0 40.0 22.5 41.0 46-47 33.43864723228724 37.5 32.5 40.0 20.0 41.0 48-49 33.01458154973466 37.0 31.5 40.0 17.5 41.0 50-51 32.26411284162221 36.5 30.5 39.5 15.0 40.5 52-53 32.24371971485482 36.0 31.0 39.0 14.5 40.0 54-55 32.30188708343062 36.0 31.0 39.0 12.5 41.0 56-57 31.9318156530061 36.0 31.0 39.0 8.5 41.0 58-59 31.508689836874588 35.5 30.0 39.0 7.5 40.0 60-61 30.987866772745747 35.0 29.5 39.0 4.0 40.0 62-63 30.383977158406623 35.0 28.5 38.0 2.0 40.0 64-65 29.819504858594854 34.0 28.0 38.0 2.0 40.0 66-67 29.248526134945273 34.0 27.5 37.0 2.0 39.5 68-69 28.63145475352468 34.0 26.0 36.5 2.0 39.0 70-71 27.95372011841654 33.5 26.0 36.0 2.0 39.0 72-73 27.315596219203194 33.0 25.0 35.0 2.0 38.0 74-75 26.637871096621325 32.5 23.5 35.0 2.0 37.0 76-77 25.892364612249253 32.0 21.0 35.0 2.0 36.5 78-79 25.109040070796254 31.0 18.5 35.0 2.0 36.0 80-81 24.430139862961973 31.0 15.0 34.0 2.0 35.5 82-83 23.691209128566115 30.0 7.5 34.0 2.0 35.0 84-85 22.997875391671062 30.0 4.0 34.0 2.0 35.0 86-87 22.244158299967427 29.0 2.0 34.0 2.0 35.0 88-89 21.39276428248167 29.0 2.0 34.0 2.0 35.0 90-91 20.465854210445908 27.0 2.0 33.5 2.0 35.0 92-93 19.512550481247352 25.5 2.0 33.0 2.0 35.0 94-95 18.49059384107163 24.5 2.0 32.5 2.0 34.0 96-97 17.234631649039667 21.0 2.0 32.0 2.0 34.0 98-99 15.705254950116888 2.0 2.0 32.0 2.0 34.0 100-101 12.3174293983183 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5149767435774706 1101 2 0.43564880454185584 1101 3 0.4191357350907019 1101 4 0.3303764174332784 1101 5 0.4101635266974313 1101 6 0.44081000920134983 1101 7 0.4192316777643157 1101 8 0.5428303344923648 1101 9 0.6590023442331301 1101 10-11 0.6902422199195826 1101 12-13 0.8424486080659648 1101 14-15 0.9294093187661403 1101 16-17 0.885868723482659 1101 18-19 0.9372792485616728 1101 20-21 0.8889519930811431 1101 22-23 0.8684904554002131 1101 24-25 0.810679492715245 1101 26-27 0.9107928872839537 1101 28-29 0.9345193963039264 1101 30-31 0.9287357199272179 1101 32-33 0.9444806144553297 1101 34-35 0.9266732129263318 1101 36-37 0.969087947915348 1101 38-39 0.9528295980877175 1101 40-41 1.0366902780698268 1101 42-43 1.0951107704268637 1101 44-45 1.1506574977383863 1101 46-47 1.1843735312821053 1101 48-49 0.974321386804462 1101 50-51 1.0662971233393748 1101 52-53 1.1359867342565266 1101 54-55 1.201055369180942 1101 56-57 1.16232776450423 1101 58-59 1.170623526337451 1101 60-61 1.3203596645011935 1101 62-63 1.1489863693726257 1101 64-65 1.0181834210472331 1101 66-67 1.1234170817998823 1101 68-69 1.2285461094243537 1101 70-71 1.2042766798108886 1101 72-73 1.412406945848474 1101 74-75 1.2638539671224258 1101 76-77 1.2992359836698348 1101 78-79 1.332813172156797 1101 80-81 1.4703101861721812 1101 82-83 1.4926391275942876 1101 84-85 1.4151566615288083 1101 86-87 1.470267312483788 1101 88-89 1.366709212916465 1101 90-91 1.2295056516627731 1101 92-93 1.251262362667255 1101 94-95 1.2982191959064266 1101 96-97 1.133607071385324 1101 98-99 1.4693116620730517 1101 100-101 1.4537260153067475 1102 1 0.42044290841432286 1102 2 0.38670880132780994 1102 3 0.3724924981057711 1102 4 0.3476689104390758 1102 5 0.3270952610417268 1102 6 0.39440554313512166 1102 7 0.4121814071268375 1102 8 0.44236566710983993 1102 9 0.5324537573186845 1102 10-11 0.6461535578402433 1102 12-13 0.6506920941396359 1102 14-15 0.8180552365595517 1102 16-17 0.7656009074284853 1102 18-19 0.6797542666406926 1102 20-21 0.7056632181448705 1102 22-23 0.8360881916875513 1102 24-25 0.8377126521977871 1102 26-27 0.7882414077260265 1102 28-29 0.6055130211660469 1102 30-31 0.8129529165466209 1102 32-33 0.8073688568822277 1102 34-35 0.6551230189877302 1102 36-37 0.8348047811281489 1102 38-39 0.701732484522239 1102 40-41 0.8182253878953247 1102 42-43 0.8794932805255442 1102 44-45 0.9003083808712091 1102 46-47 0.7088927023498286 1102 48-49 1.010474180104623 1102 50-51 0.8840530054130156 1102 52-53 0.7011133056704892 1102 54-55 0.7634857466855252 1102 56-57 0.8740557537517795 1102 58-59 0.8331540232731385 1102 60-61 0.8242513108974414 1102 62-63 0.9553757056716492 1102 64-65 0.9490218493776084 1102 66-67 0.760245373094925 1102 68-69 0.8472547011598657 1102 70-71 1.029107074846646 1102 72-73 1.116339315697779 1102 74-75 1.1461532351959711 1102 76-77 1.1382107935661843 1102 78-79 1.2988162565474823 1102 80-81 1.3813745474896493 1102 82-83 1.4521573216643695 1102 84-85 1.4209069318010528 1102 86-87 1.639737186259346 1102 88-89 1.6114702228581024 1102 90-91 1.5417874707410668 1102 92-93 1.5800413347561673 1102 94-95 1.7668560194040417 1102 96-97 1.8580401388292067 1102 98-99 1.7826562628371203 1102 100-101 1.542181829447097 1103 1 0.36510919794434216 1103 2 0.4500067503699938 1103 3 0.36154998925970006 1103 4 0.3585115889966346 1103 5 0.1951029812507059 1103 6 0.34056374086564745 1103 7 0.20706344100258178 1103 8 0.3650082857692283 1103 9 0.4948288308048703 1103 10-11 0.5387666711032466 1103 12-13 0.5598354393416827 1103 14-15 0.7490170459280705 1103 16-17 0.7203999712422231 1103 18-19 0.621653038728148 1103 20-21 0.6003921789115694 1103 22-23 0.6760546930796281 1103 24-25 0.7517892774903387 1103 26-27 0.698488432228821 1103 28-29 0.6697704502033588 1103 30-31 0.7277893612572797 1103 32-33 0.7393317421994112 1103 34-35 0.8662668522489696 1103 36-37 0.9475436805546806 1103 38-39 0.901947674255247 1103 40-41 0.860328812074215 1103 42-43 0.8839662320634858 1103 44-45 0.8633378973598411 1103 46-47 0.7458631858611895 1103 48-49 0.7465173511582819 1103 50-51 0.8985089858019109 1103 52-53 0.9370899231984637 1103 54-55 1.000116807215953 1103 56-57 0.96216430255215 1103 58-59 0.8896551935059733 1103 60-61 1.104535938142238 1103 62-63 0.9759906602488719 1103 64-65 1.2100669615757624 1103 66-67 1.1818453198521048 1103 68-69 1.3641537505536725 1103 70-71 1.3540851676444454 1103 72-73 1.3242358915188852 1103 74-75 1.319253409344359 1103 76-77 1.3875618966908725 1103 78-79 1.3675688216312842 1103 80-81 1.5405622617088106 1103 82-83 1.5409452710393197 1103 84-85 1.5978977141377477 1103 86-87 1.6903816462329466 1103 88-89 1.490728261969391 1103 90-91 1.5828620883356024 1103 92-93 1.5646036898635955 1103 94-95 1.453516474408314 1103 96-97 1.764166390867409 1103 98-99 1.8360821055711423 1103 100-101 1.6769804495706655 1104 1 0.39841021473983673 1104 2 0.37790287587868576 1104 3 0.4359845615978344 1104 4 0.28933971186769014 1104 5 0.374852879061244 1104 6 0.5222434425866567 1104 7 0.3667347857861003 1104 8 0.5017229686524374 1104 9 0.5544775109673381 1104 10-11 0.6468218247715356 1104 12-13 0.6696874140361118 1104 14-15 0.74168059583684 1104 16-17 0.8641244621615627 1104 18-19 0.7752605626538909 1104 20-21 0.7963195501416678 1104 22-23 0.8223764270605471 1104 24-25 0.8216764808532488 1104 26-27 0.8069260617246741 1104 28-29 0.850223878380433 1104 30-31 0.8613005762713613 1104 32-33 0.872423189889254 1104 34-35 0.8268452702650393 1104 36-37 0.8772676688458958 1104 38-39 0.8834340396397309 1104 40-41 0.8821331625887865 1104 42-43 0.9204520088962695 1104 44-45 0.8775515451039482 1104 46-47 0.876898137885533 1104 48-49 0.7421549496620514 1104 50-51 0.795857415527756 1104 52-53 0.8497747603020898 1104 54-55 0.9399439766498077 1104 56-57 0.9304360266907672 1104 58-59 0.8910809206818726 1104 60-61 1.001518613199945 1104 62-63 1.107242159565704 1104 64-65 1.1536255156822044 1104 66-67 1.146268983703159 1104 68-69 1.2493100130997163 1104 70-71 1.3323974513558348 1104 72-73 1.4148177233003807 1104 74-75 1.2947297595821219 1104 76-77 1.2973544284912855 1104 78-79 1.5460772561317704 1104 80-81 1.4675675715874874 1104 82-83 1.4139588262707186 1104 84-85 1.4818399128973674 1104 86-87 1.6508205832903613 1104 88-89 1.5319844531442293 1104 90-91 1.4340512193833028 1104 92-93 1.610754793518062 1104 94-95 1.7181865187491852 1104 96-97 1.8320067835928135 1104 98-99 1.878030693469226 1104 100-101 1.4886310746854168 1105 1 0.38452075870173275 1105 2 0.3110867326269542 1105 3 0.27145604965900105 1105 4 0.26003486577117485 1105 5 0.2612901244858108 1105 6 0.39129927355683947 1105 7 0.2416864169930335 1105 8 0.3193481061757808 1105 9 0.422667974557676 1105 10-11 0.46088207349666277 1105 12-13 0.47147031694434105 1105 14-15 0.5460583076537446 1105 16-17 0.4396916634132779 1105 18-19 0.46527312893882566 1105 20-21 0.5095873070045585 1105 22-23 0.5533681477966752 1105 24-25 0.6595926062897419 1105 26-27 0.6194287510570078 1105 28-29 0.5675329032585097 1105 30-31 0.7754814246511685 1105 32-33 0.7521317552236226 1105 34-35 0.6253410154963674 1105 36-37 0.7051482837309209 1105 38-39 0.7435202957858138 1105 40-41 0.8196430724600035 1105 42-43 0.8777090051379801 1105 44-45 1.0363622132081716 1105 46-47 0.8108324996012612 1105 48-49 0.8579677299649404 1105 50-51 0.8744334555028601 1105 52-53 0.8731863534347397 1105 54-55 0.9555116544375082 1105 56-57 0.906165375577249 1105 58-59 1.0366542737935447 1105 60-61 1.0777985022140903 1105 62-63 1.0908796934512885 1105 64-65 1.2805733662659193 1105 66-67 1.4445273622910797 1105 68-69 1.1770697461705986 1105 70-71 1.2453241009481957 1105 72-73 1.2886854311145406 1105 74-75 1.4587109213627159 1105 76-77 1.5451541137737657 1105 78-79 1.5953060869029514 1105 80-81 1.6343968587672677 1105 82-83 1.7129129238648133 1105 84-85 1.7476871832750227 1105 86-87 1.785544435005221 1105 88-89 1.8182342819870847 1105 90-91 1.605639164998319 1105 92-93 1.49702368379981 1105 94-95 1.7227481750189284 1105 96-97 1.9872682384242175 1105 98-99 2.055272761908098 1105 100-101 1.7882337471430407 1106 1 0.22872928996298114 1106 2 0.17408752611869005 1106 3 0.12683063681398465 1106 4 0.15363111104116456 1106 5 0.23555787234517567 1106 6 0.07009344255487093 1106 7 0.34102460844756877 1106 8 0.24820871361475128 1106 9 0.20664039962045422 1106 10-11 0.2147573743544271 1106 12-13 0.243154025673185 1106 14-15 0.47716643488131893 1106 16-17 0.40188720886502693 1106 18-19 0.19290469589967074 1106 20-21 0.36292173926844384 1106 22-23 0.20504416348808263 1106 24-25 0.10849304223900447 1106 26-27 0.1319203166733658 1106 28-29 0.4200700349649438 1106 30-31 0.10880096245323045 1106 32-33 0.2711584760420678 1106 34-35 -0.12382229992947913 1106 36-37 0.029093522434969543 1106 38-39 0.12888221880015038 1106 40-41 0.42062671478183233 1106 42-43 -0.013870055978465246 1106 44-45 0.16418121353424198 1106 46-47 0.3041871633913331 1106 48-49 0.6801433187540198 1106 50-51 0.41320016799058834 1106 52-53 0.3453250869515685 1106 54-55 0.4192547993411537 1106 56-57 -0.41535006955401954 1106 58-59 -0.13831359858684777 1106 60-61 0.5212433290940481 1106 62-63 0.5372313591870856 1106 64-65 0.6271900554138057 1106 66-67 0.20177865477791457 1106 68-69 0.24919523126545684 1106 70-71 0.006040912041250124 1106 72-73 0.4164963033334743 1106 74-75 0.8231964490912631 1106 76-77 0.8432609244835767 1106 78-79 1.103362847320593 1106 80-81 1.135954628169646 1106 82-83 0.8237762607771337 1106 84-85 0.9149574240680352 1106 86-87 0.7884681190686038 1106 88-89 0.664908849540371 1106 90-91 0.8878691516352397 1106 92-93 0.7810254799936907 1106 94-95 0.7925662200764414 1106 96-97 0.7342897779866568 1106 98-99 0.7849705565288172 1106 100-101 0.3332523876020712 1107 1 0.22431541310423242 1107 2 0.08151590287081945 1107 3 0.21073718897169158 1107 4 0.145765972794635 1107 5 0.21539809410825228 1107 6 0.28390804685924564 1107 7 0.2780324245665682 1107 8 0.20846452055734943 1107 9 0.33541120522727397 1107 10-11 0.3453809543281068 1107 12-13 0.4283878045914804 1107 14-15 0.5349285714342429 1107 16-17 0.5949083079745989 1107 18-19 0.48129059624844217 1107 20-21 0.588196584061258 1107 22-23 0.5181379091223093 1107 24-25 0.5821053686189543 1107 26-27 0.46685734532266565 1107 28-29 0.6892138892993529 1107 30-31 0.6682533439308145 1107 32-33 0.7085417489591848 1107 34-35 0.5124184262416591 1107 36-37 0.5206320344066953 1107 38-39 0.5988253013265066 1107 40-41 0.6572412433514643 1107 42-43 0.7877187191040704 1107 44-45 0.6802129583988616 1107 46-47 0.7720029637653525 1107 48-49 0.7736737278441552 1107 50-51 0.7555773992854498 1107 52-53 0.8541513088175847 1107 54-55 0.9669869851339072 1107 56-57 0.9360963352518219 1107 58-59 0.7622289525708652 1107 60-61 0.7642266833831108 1107 62-63 0.9764732275066805 1107 64-65 0.912870399675981 1107 66-67 0.9141030739611047 1107 68-69 1.081619717114826 1107 70-71 1.2421542188061494 1107 72-73 1.2670085851059127 1107 74-75 1.0920063598027028 1107 76-77 1.2745097449579355 1107 78-79 1.4440461572472358 1107 80-81 1.4905016535591677 1107 82-83 1.4086746867690962 1107 84-85 1.467480322183981 1107 86-87 1.5646005862318333 1107 88-89 1.7465346280272946 1107 90-91 1.6774593936187578 1107 92-93 1.7575118661232025 1107 94-95 1.6856318041530294 1107 96-97 1.771032920806686 1107 98-99 1.7704838370684204 1107 100-101 1.6310250142739342 1108 1 0.2282485113425956 1108 2 0.20044947232944565 1108 3 0.22584654086049483 1108 4 0.2226932863876243 1108 5 0.12642451183728554 1108 6 0.2186441923017597 1108 7 0.329604678996418 1108 8 0.27479998453354426 1108 9 0.3928615914915241 1108 10-11 0.4365855978628588 1108 12-13 0.30025631110810735 1108 14-15 0.5049883573153906 1108 16-17 0.5156695529415529 1108 18-19 0.4689854594627434 1108 20-21 0.4598807692123259 1108 22-23 0.34057416029391874 1108 24-25 0.4809487350043753 1108 26-27 0.5244633024558638 1108 28-29 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70-71 -2.279010307591953 2316 72-73 -2.3039593098470483 2316 74-75 -2.2345412352361897 2316 76-77 -2.4027708509589516 2316 78-79 -2.594295846872953 2316 80-81 -2.898706163331976 2316 82-83 -2.806347404166633 2316 84-85 -2.927886423435009 2316 86-87 -2.887396517588577 2316 88-89 -2.948799363872599 2316 90-91 -2.716924594919913 2316 92-93 -2.778056184639098 2316 94-95 -3.29874877514691 2316 96-97 -3.58993554453534 2316 98-99 -3.4046999969995806 2316 100-101 -2.7964448504129003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12395.0 3 4313.0 4 4718.0 5 5358.0 6 6638.0 7 8352.0 8 10727.0 9 13517.0 10 17097.0 11 21318.0 12 25868.0 13 29852.0 14 33771.0 15 39136.0 16 45399.0 17 51608.0 18 49680.0 19 48461.0 20 50809.0 21 54248.0 22 59800.0 23 66700.0 24 73593.0 25 81634.0 26 91400.0 27 104179.0 28 116744.0 29 133356.0 30 151548.0 31 175951.0 32 205648.0 33 243873.0 34 291378.0 35 344925.0 36 377132.0 37 308269.0 38 105900.0 39 3815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35208007218489 17.24342509232113 14.379581823475657 24.024913012018324 2 16.150944332690898 19.062070493899373 38.68181538801597 26.105169785393755 3 19.772247168961897 22.346067714044953 28.929024922370104 28.952660194623046 4 13.530500110676519 15.512085962321501 34.68561664136162 36.27179728564036 5 14.727692042965055 36.388990579885046 32.978197578740584 15.90511979840931 6 34.67360738013939 34.28950404156811 16.05192435426377 14.984964224028735 7 30.50597289060225 31.899041857809742 18.86588604364838 18.729099207939633 8 28.424818586980937 32.922783087782655 18.732474653212584 19.91992367202383 9 27.320203961300983 16.12620574026794 18.710522863473656 37.84306743495742 10-11 26.009528349854662 25.335408701259787 26.072645952787397 22.58241699609816 12-13 27.297902114844057 22.564090509764924 26.294615895334744 23.843391480056276 14-15 23.531650633805608 24.032135404089374 24.587476707598817 27.8487372545062 16-17 23.4462597907811 27.225302692954095 25.645672160091372 23.68276535617343 18-19 23.86461976069575 25.229042710903705 27.90017195325109 23.006165575149453 20-21 23.692068695687034 24.5914621008757 28.381345688088956 23.33512351534831 22-23 23.61398743357065 24.490105162386065 28.43437226965631 23.461535134386978 24-25 23.6292198151201 24.819919177050252 28.17608755088927 23.37477345694038 26-27 22.706902844818124 25.23556597339845 28.005711354511376 24.051819827272052 28-29 23.142956191895728 25.934023369475245 27.709273437396355 23.213747001232672 30-31 23.055300194503875 25.161555327164862 27.533937219353238 24.24920725897803 32-33 22.610665198883936 25.175713579331145 27.683297515993065 24.530323705791854 34-35 23.891145925724018 25.295037259135228 27.354276904528064 23.45953991061269 36-37 24.159564800199348 24.519919210470682 27.358800743291354 23.96171524603861 38-39 23.255532979306086 25.100947938156292 27.22998454008297 24.413534542454652 40-41 23.926125088022534 24.890999122802466 27.548111776074137 23.634764013100867 42-43 23.547549019607843 24.995314302191467 27.188336216839676 24.268800461361014 44-45 23.31210216789086 25.310485795337023 27.704458371776354 23.67295366499576 46-47 24.095825625660282 25.795105365145858 26.554184731318912 23.55488427787494 48-49 23.645015795746875 25.889904429857648 26.2732487635902 24.191831010805277 50-51 23.919614707322484 26.106986377448276 26.434963122435473 23.538435792793766 52-53 24.44333550700447 25.332839788905975 26.571126036601854 23.652698667487705 54-55 23.421164381448843 25.91301805870311 26.944285004683255 23.721532555164796 56-57 23.61161103039527 25.94963321821055 26.67800740670161 23.760748344692573 58-59 23.628513156551808 25.24667994056155 26.51238502829208 24.612421874594563 60-61 22.812538743876665 25.837473221674077 27.102938335606307 24.247049698842947 62-63 23.356700147632985 25.89750966994615 27.15584952102163 23.589940661399236 64-65 22.84875793139434 25.970476907066114 26.864284870027472 24.316480291512075 66-67 22.711238142626783 25.915029792170476 27.0370379446033 24.336694120599446 68-69 23.691358994557795 25.195097042210783 27.207985072314088 23.905558890917334 70-71 23.25722115581745 25.68255431960252 26.51488348314422 24.545341041435808 72-73 23.29040511038511 25.802995743988838 26.578754371742775 24.327844773883278 74-75 23.249372209037016 26.3485215158791 26.483667054677863 23.918439220406025 76-77 23.557545373500286 25.605869001857958 26.697794093054743 24.138791531587014 78-79 23.592644273393326 25.981065993147485 26.57833717960526 23.847952553853933 80-81 23.93336009461097 26.00351548058234 26.133338906597615 23.92978551820908 82-83 23.556882643962922 26.091335789817794 26.33288112648512 24.018900439734164 84-85 23.364103548664303 26.33097612843581 26.391354820594998 23.913565502304884 86-87 23.969489856739553 26.403569655049143 25.61028574970468 24.016654738506624 88-89 23.48378086658725 26.492226887060827 25.49236732244512 24.531624923906804 90-91 23.329077141742427 26.3891087632851 25.798634270012677 24.483179824959795 92-93 23.67747506085119 25.73060716535305 26.198256303214606 24.393661470581154 94-95 23.25033381494972 25.44245260981286 26.510386475519752 24.796827099717664 96-97 23.73474708351303 24.911737941748267 26.444487797450527 24.909027177288177 98-99 23.32719668649904 25.388980273828043 25.910311729164835 25.373511310508086 100-101 23.971366648425494 24.5086638836619 26.40747182258157 25.112497645331032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 410.5 2 329.0 3 448.5 4 444.5 5 438.0 6 690.5 7 1517.5 8 2544.5 9 2899.0 10 2487.5 11 2083.5 12 2104.5 13 2332.5 14 2598.0 15 2905.5 16 3155.0 17 3519.5 18 3992.0 19 4477.5 20 5166.5 21 6084.0 22 7237.5 23 8625.5 24 10471.5 25 13047.5 26 16257.5 27 19908.0 28 22976.5 29 25674.5 30 28995.0 31 36190.0 32 49256.5 33 51375.0 34 45352.5 35 46246.5 36 50271.0 37 55717.0 38 61552.5 39 66354.5 40 72480.5 41 81475.5 42 88531.5 43 94580.0 44 100395.0 45 109755.0 46 116961.5 47 122869.0 48 127882.5 49 127454.0 50 128356.0 51 129305.5 52 130726.0 53 137442.5 54 146319.5 55 143548.5 56 134335.0 57 127344.5 58 123238.5 59 112132.0 60 91273.0 61 76516.5 62 66748.5 63 52054.0 64 37915.5 65 29494.0 66 22380.5 67 16854.5 68 13029.5 69 10057.0 70 7900.5 71 5905.5 72 4289.0 73 3469.5 74 2730.0 75 2066.0 76 1572.0 77 1127.0 78 753.5 79 501.0 80 343.0 81 202.0 82 108.0 83 63.5 84 44.5 85 33.0 86 26.0 87 18.0 88 19.0 89 16.0 90 10.0 91 7.5 92 3.0 93 4.5 94 4.5 95 1.0 96 1.0 97 1.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0069182009218502726 2 0.10512782817494977 3 0.11386205683878572 4 0.11694642141644399 5 0.15733141929774494 6 0.09731602630069384 7 0.027096286943913566 8 0.02649094436325167 9 0.02320479892537279 10-11 0.05110820931016889 12-13 0.054884393980012165 14-15 0.028033126652080794 16-17 0.03215522136801658 18-19 0.03767536918690961 20-21 0.05912179204464545 22-23 0.0328614543787888 24-25 0.05125233849604077 26-27 0.057118396361026315 28-29 0.05338545044694461 30-31 0.06748128482521454 32-33 0.05040197629939667 34-35 0.0626961958542681 36-37 0.052722456191933956 38-39 0.031982266344970325 40-41 0.03597464479362142 42-43 0.03199667926355751 44-45 0.006673181305868076 46-47 0.012524826252266431 48-49 0.008546860722202523 50-51 0.007682085606971241 52-53 0.009339571244497868 54-55 0.010579082242996043 56-57 0.014254376482729002 58-59 0.018679142488995736 60-61 0.02363718648298843 62-63 0.010752037266042298 64-65 0.008647751152312842 66-67 0.009498113348956937 68-69 0.011170011905070753 70-71 0.030872471613756843 72-73 0.027571913257290775 74-75 0.02873935966285301 76-77 0.022657108019059645 78-79 0.004799501889533627 80-81 0.004828327726708003 82-83 0.005649864086177723 84-85 0.00492921815681832 86-87 0.0123230453920458 88-89 0.03813658258169963 90-91 0.03519634718991326 92-93 0.029359115162102097 94-95 0.04669785622248934 96-97 0.04189835433295571 98-99 0.025222607527579124 100-101 0.07474539579315732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3469110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.11382376082197 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00409505242496 29.192498502820513 2 13.864945127201075 11.400818208264246 3 5.524707865980993 6.814255965959082 4 2.874033335323964 4.726500001249472 5 1.7091358671068597 3.513455541176554 6 1.051438225135712 2.593718753017267 7 0.707572835094367 2.036371738801047 8 0.5168108210580598 1.6998455211733414 9 0.40813597532440526 1.5102027503944906 >10 2.0857861557941604 15.59215949241648 >50 0.15235411562686715 4.326219753326446 >100 0.08674945568343342 6.742455383777054 >500 0.008245122202305511 2.4078324258925714 >1k 0.005144392483873838 4.953694014888594 >5k 7.751824290768797E-4 2.176335149954711 >10k+ 7.047112991607998E-5 0.31363679688823465 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10825 0.31203968741262167 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 8192 0.2361412581324893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8188 0.23602595478379182 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7814 0.2252450916805751 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7730 0.22282372135792755 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6979 0.20117551763997105 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6502 0.18742559330779363 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 6341 0.18278463352271906 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 6051 0.17442514074215001 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 6021 0.17356036562691873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.1657197379154884 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5548 0.1599257446434388 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4936 0.1422843322927206 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4890 0.1409583437826993 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4611 0.13291593521104836 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4405 0.1269778127531269 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4319 0.12449879075613053 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4282 0.1234322347806786 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4270 0.1230863247345861 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3971 0.11446739941944764 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3770 0.10867340614739804 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3686 0.10625203582475043 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3660 0.10550256405821665 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3493 0.10068864925009584 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7651674348752275E-5 2 0.0 0.0 0.0 0.0 5.7651674348752275E-5 3 0.0 0.0 0.0 0.0 5.7651674348752275E-5 4 0.0 0.0 0.0 0.0 8.647751152312841E-5 5 0.0 0.0 0.0 0.0 8.647751152312841E-5 6 0.0 0.0 0.0 0.0 8.647751152312841E-5 7 0.0 0.0 0.0 0.0 8.647751152312841E-5 8 0.0 0.0 0.0 0.0 8.647751152312841E-5 9 0.0 0.0 0.0 2.8825837174376137E-5 8.647751152312841E-5 10-11 0.0 0.0 0.0 2.8825837174376137E-5 1.1530334869750455E-4 12-13 0.0 0.0 0.0 2.8825837174376137E-5 2.882583717437613E-4 14-15 2.8825837174376137E-5 0.0 0.0 4.3238755761564206E-5 3.8914880185407784E-4 16-17 2.8825837174376137E-5 0.0 0.0 5.7651674348752275E-5 5.765167434875227E-4 18-19 2.8825837174376137E-5 0.0 0.0 5.7651674348752275E-5 6.629942550106511E-4 20-21 2.8825837174376137E-5 0.0 0.0 1.1530334869750455E-4 7.638846851209676E-4 22-23 2.8825837174376137E-5 0.0 0.0 3.026712903309494E-4 7.782976037081557E-4 24-25 2.8825837174376137E-5 0.0 0.0 6.774071735978392E-4 8.071234408825318E-4 26-27 2.8825837174376137E-5 0.0 0.0 0.0011674464055622335 8.359492780569079E-4 28-29 8.647751152312841E-5 0.0 0.0 0.0035311650538610764 8.936009524056602E-4 30-31 8.647751152312841E-5 0.0 0.0 0.011544747788337643 8.936009524056602E-4 32-33 8.647751152312841E-5 0.0 0.0 0.027355719478482955 9.224267895800364E-4 34-35 8.647751152312841E-5 0.0 0.0 0.052189178204207994 9.512526267544126E-4 36-37 8.647751152312841E-5 0.0 0.0 0.09345336411932743 9.512526267544126E-4 38-39 8.647751152312841E-5 0.0 0.0 0.15792234895981966 9.94491382515977E-4 40-41 1.0089043011031648E-4 2.8825837174376137E-5 0.0 0.23076523949946814 0.0010089043011031649 42-43 1.2971626728469263E-4 2.8825837174376137E-5 0.0 0.30473233768891733 0.001037730138277541 44-45 1.441291858718807E-4 2.8825837174376137E-5 0.0 0.39675882286811315 0.0011386205683878575 46-47 1.441291858718807E-4 2.8825837174376137E-5 0.0 0.5087039615348028 0.0012250980799109858 48-49 1.441291858718807E-4 2.8825837174376137E-5 0.0 0.6278123207393251 0.00126833683567255 50-51 1.441291858718807E-4 2.8825837174376137E-5 0.0 0.7643603114343449 0.0013836401843700546 52-53 1.441291858718807E-4 2.8825837174376137E-5 0.0 0.894278359579258 0.0015277693702419352 54-55 1.441291858718807E-4 2.8825837174376137E-5 0.0 1.0327288555277865 0.0015565952074163114 56-57 1.7295502304625682E-4 2.8825837174376137E-5 0.0 1.1975838183280438 0.0015710081260034993 58-59 1.7295502304625682E-4 2.8825837174376137E-5 0.0 1.3707550351531084 0.0015854210445906875 60-61 1.7295502304625682E-4 2.8825837174376137E-5 0.0 1.5681687810418234 0.0016286598003522517 62-63 1.7295502304625682E-4 2.8825837174376137E-5 0.0 1.761042457575574 0.001671898556113816 64-65 1.7295502304625682E-4 2.8825837174376137E-5 0.0 1.958600332650161 0.001671898556113816 66-67 2.0178086022063296E-4 2.8825837174376137E-5 0.0 2.1952892816889635 0.001671898556113816 68-69 2.0178086022063296E-4 2.8825837174376137E-5 0.0 2.4597519248452775 0.001671898556113816 70-71 2.0178086022063296E-4 2.8825837174376137E-5 0.0 2.732891144991078 0.0017151373118753802 72-73 2.0178086022063296E-4 2.8825837174376137E-5 0.0 3.0213224717578857 0.001888092334921637 74-75 2.0178086022063296E-4 2.8825837174376137E-5 0.0 3.3120310396614694 0.0019745698464447656 76-77 2.0178086022063296E-4 2.8825837174376137E-5 0.0 3.6291729002539554 0.0020178086022063298 78-79 2.0178086022063296E-4 2.8825837174376137E-5 0.0 3.9878960309704796 0.0020178086022063298 80-81 2.306066973950091E-4 2.8825837174376137E-5 0.0 4.356535249674989 0.002075460276555082 82-83 2.306066973950091E-4 2.8825837174376137E-5 0.0 4.747759511805621 0.0021763507066653986 84-85 2.450196159821972E-4 2.8825837174376137E-5 0.0 5.151191515979603 0.0021907636252525863 86-87 2.594325345693852E-4 2.8825837174376137E-5 0.0 5.561584959831196 0.0022484152996013387 88-89 2.594325345693852E-4 2.8825837174376137E-5 0.0 5.988841518429799 0.002306066973950091 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6725 0.0 49.00995 1 GTATCAA 12110 0.0 41.47278 1 ATCAACG 15590 0.0 32.15421 3 TATCAAC 15655 0.0 31.990324 2 TCAACGC 15840 0.0 31.52662 4 AGGTAAG 2250 0.0 30.649881 2 CAACGCA 16385 0.0 30.47798 5 AACGCAG 17065 0.0 29.43649 6 TAAGGTG 2810 0.0 28.942305 5 AAGGTAA 2420 0.0 27.710672 1 GTGTAAG 1380 0.0 26.537191 1 ACGCAGA 19000 0.0 26.038034 7 CGCAGAG 18910 0.0 25.909895 8 GTAAGGT 3055 0.0 25.842836 4 GGTAAGG 3130 0.0 24.463854 3 GTACATG 12500 0.0 24.19861 1 TAAGGTA 1490 0.0 23.93968 4 TACATGG 12525 0.0 23.846533 2 TGGTATC 3365 0.0 23.603409 2 GCAGAGT 20130 0.0 23.37168 9 >>END_MODULE