##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938623_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3469110 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801792678813875 31.0 31.0 34.0 30.0 34.0 2 32.026230358795196 31.0 31.0 34.0 30.0 34.0 3 32.083348178639476 33.0 31.0 34.0 30.0 34.0 4 35.76816935755857 37.0 35.0 37.0 35.0 37.0 5 35.66240159579834 37.0 35.0 37.0 33.0 37.0 6 35.66679407686698 37.0 35.0 37.0 33.0 37.0 7 35.601104606080526 37.0 35.0 37.0 33.0 37.0 8 35.565978593933316 37.0 35.0 37.0 33.0 37.0 9 37.15134486943337 39.0 37.0 39.0 33.0 39.0 10-11 37.04759996079686 39.0 37.0 39.0 33.0 39.0 12-13 36.94387177114591 39.0 37.0 39.0 33.0 39.0 14-15 37.990105243131524 40.0 37.0 41.0 33.0 41.0 16-17 37.90104940460233 40.0 37.0 41.0 33.0 41.0 18-19 38.13233480633362 40.0 37.0 41.0 33.0 41.0 20-21 38.08504112005673 40.0 37.0 41.0 33.0 41.0 22-23 37.9887446636169 40.0 37.0 41.0 33.0 41.0 24-25 37.85467857750258 40.0 37.0 41.0 32.0 41.0 26-27 37.649227755822096 40.0 37.0 41.0 32.0 41.0 28-29 37.53389630193335 39.5 36.5 41.0 32.0 41.0 30-31 37.25615575752859 39.0 36.0 41.0 31.0 41.0 32-33 37.033966781105235 39.0 36.0 41.0 31.0 41.0 34-35 36.93346780586375 39.0 35.5 41.0 30.5 41.0 36-37 36.86770454093413 39.0 35.0 41.0 30.5 41.0 38-39 36.679990977512965 39.0 35.0 41.0 30.0 41.0 40-41 36.48271977538907 39.0 35.0 40.5 30.0 41.0 42-43 36.23544324048531 39.0 35.0 40.0 30.0 41.0 44-45 35.92391506755335 38.0 35.0 40.0 29.0 41.0 46-47 35.51655366938495 38.0 34.5 40.0 27.5 41.0 48-49 35.112994975656584 38.0 34.0 40.0 26.5 41.0 50-51 34.695288416913854 38.0 33.5 40.0 26.0 41.0 52-53 34.15966991533851 37.0 33.0 40.0 24.0 41.0 54-55 33.78145287984526 37.0 33.0 40.0 23.0 41.0 56-57 33.409130295666614 36.5 32.0 39.5 23.0 41.0 58-59 32.89641853386027 36.0 31.5 39.0 21.5 40.0 60-61 32.457993260519274 35.5 31.0 39.0 20.0 40.0 62-63 31.95147372092554 35.0 31.0 38.0 18.5 40.0 64-65 31.396227850947362 35.0 30.0 38.0 17.0 40.0 66-67 30.870343978715 34.0 29.0 37.0 13.0 39.5 68-69 30.362267555655485 34.0 29.0 36.5 9.0 39.0 70-71 29.772512978833188 34.0 28.5 36.0 7.5 39.0 72-73 29.27733092925851 34.0 28.0 35.5 6.5 38.5 74-75 28.695888282585447 33.0 27.0 35.0 2.0 37.5 76-77 27.537915056023014 31.5 26.0 34.0 2.0 36.0 78-79 28.221824473712275 33.0 27.0 35.0 2.0 36.0 80-81 27.881097024885346 33.0 26.0 35.0 2.0 36.0 82-83 27.45715039304029 33.0 26.0 35.0 2.0 36.0 84-85 26.921324777824857 32.0 25.0 35.0 2.0 35.0 86-87 26.341350951685016 32.0 24.5 34.0 2.0 35.0 88-89 25.584969199592976 31.0 22.0 34.0 2.0 35.0 90-91 24.990221843642892 31.0 19.5 34.0 2.0 35.0 92-93 24.17732992035421 31.0 16.5 34.0 2.0 35.0 94-95 23.375705007912693 30.5 4.5 34.0 2.0 35.0 96-97 22.312635517467015 30.0 2.0 34.0 2.0 35.0 98-99 20.93854619772795 29.0 2.0 34.0 2.0 35.0 100-101 17.0316065215574 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21683998496691714 1101 2 0.2047950532696703 1101 3 0.22784210437636432 1101 4 0.21106927585990576 1101 5 0.1181155047688236 1101 6 0.17157212204182315 1101 7 0.14717132043561065 1101 8 0.21738914831901468 1101 9 0.23747584354839546 1101 10-11 0.19692447423346238 1101 12-13 0.27123348808067504 1101 14-15 0.41544051937522397 1101 16-17 0.3764376575974495 1101 18-19 0.43067787855211037 1101 20-21 0.3135870630534967 1101 22-23 0.37970942768364324 1101 24-25 0.4063164843888316 1101 26-27 0.2993375168317627 1101 28-29 0.2560224207555706 1101 30-31 0.38788993471003863 1101 32-33 0.3550638177658172 1101 34-35 0.4515677330840546 1101 36-37 0.5264987020214562 1101 38-39 0.5361261681938174 1101 40-41 0.5773901490106752 1101 42-43 0.705629759869403 1101 44-45 0.6439651140332217 1101 46-47 0.5970397427456717 1101 48-49 0.6155229567989196 1101 50-51 0.684226605287968 1101 52-53 0.7559151993774549 1101 54-55 0.6644282076013468 1101 56-57 0.5810337552409379 1101 58-59 0.7290510211141239 1101 60-61 0.7255341827901418 1101 62-63 0.8783521905998732 1101 64-65 0.7795542684360512 1101 66-67 0.663933470858062 1101 68-69 0.8618439915250065 1101 70-71 0.9316850773493108 1101 72-73 1.0241709767346023 1101 74-75 0.8014226631030041 1101 76-77 0.8808674752035692 1101 78-79 1.0714445023682195 1101 80-81 1.1567682289719556 1101 82-83 1.22507716366853 1101 84-85 1.2695329478286652 1101 86-87 1.3320689920178204 1101 88-89 1.2446130514453095 1101 90-91 1.1996481446258187 1101 92-93 1.3418452055715342 1101 94-95 1.0992328601617238 1101 96-97 1.1651651468735693 1101 98-99 1.4157106578707577 1101 100-101 1.5254080096833569 1102 1 0.30720322721119686 1102 2 0.3408355831493637 1102 3 0.2590714230711626 1102 4 0.20721579628737175 1102 5 0.17221177282044664 1102 6 0.18179608277199577 1102 7 0.2126364626207291 1102 8 0.2142287892221617 1102 9 0.2882526936349521 1102 10-11 0.34915417459649944 1102 12-13 0.33935802697431683 1102 14-15 0.49853165773269836 1102 16-17 0.4742595920162529 1102 18-19 0.3983849563978268 1102 20-21 0.37790884407454683 1102 22-23 0.36150809860161104 1102 24-25 0.4060842876598585 1102 26-27 0.41696479131878306 1102 28-29 0.25717285830442194 1102 30-31 0.3802741011392996 1102 32-33 0.5098625653826687 1102 34-35 0.41551378090033353 1102 36-37 0.5635732971609357 1102 38-39 0.54604552479978 1102 40-41 0.533351934706694 1102 42-43 0.4514047016147771 1102 44-45 0.5770421909293262 1102 46-47 0.6368879154861631 1102 48-49 0.5145595640787306 1102 50-51 0.6262403800416152 1102 52-53 0.574828458422445 1102 54-55 0.4211668890380267 1102 56-57 0.5225016439764261 1102 58-59 0.7533903150461398 1102 60-61 0.5712248927295818 1102 62-63 0.6457590927686176 1102 64-65 0.5553488779657059 1102 66-67 0.5161007164186593 1102 68-69 0.809619495728839 1102 70-71 0.6355113014269165 1102 72-73 0.7517849932723415 1102 74-75 0.8304932278989483 1102 76-77 0.8697934808719836 1102 78-79 1.051781420461122 1102 80-81 1.0464773849920874 1102 82-83 1.0767592888118855 1102 84-85 1.054373642428331 1102 86-87 0.7572826075299908 1102 88-89 0.8214090835565706 1102 90-91 0.9731566709846682 1102 92-93 1.3708117246841631 1102 94-95 1.286512716113922 1102 96-97 1.195920621712684 1102 98-99 1.3500287500408987 1102 100-101 1.5517243085606296 1103 1 0.25868570699556415 1103 2 0.2275892130895727 1103 3 0.2990589997463715 1103 4 0.1788362637149632 1103 5 0.1763491836664528 1103 6 0.14890754883524693 1103 7 0.2612704515062916 1103 8 0.19232713314859495 1103 9 0.2461464963924911 1103 10-11 0.23604645771519728 1103 12-13 0.29799778383209485 1103 14-15 0.4062551943870574 1103 16-17 0.3648594611276579 1103 18-19 0.43951226220018214 1103 20-21 0.41070586691349575 1103 22-23 0.4157103991351505 1103 24-25 0.45963214519094464 1103 26-27 0.44121523223589776 1103 28-29 0.43178712516188256 1103 30-31 0.36274833937873296 1103 32-33 0.3370286053369682 1103 34-35 0.42603755714737446 1103 36-37 0.4860744895782716 1103 38-39 0.38926205669555713 1103 40-41 0.44766427040267587 1103 42-43 0.541471033295366 1103 44-45 0.5590893773790313 1103 46-47 0.5294622058591969 1103 48-49 0.5317026430666729 1103 50-51 0.746621842666265 1103 52-53 0.7745999579843001 1103 54-55 0.7190299662512132 1103 56-57 0.6049004548867671 1103 58-59 0.6216281995314077 1103 60-61 0.5566025285265042 1103 62-63 0.6302271637146823 1103 64-65 0.44005876005504163 1103 66-67 0.5580044800205357 1103 68-69 0.4767187603123766 1103 70-71 0.4441116807820116 1103 72-73 0.5573239104464776 1103 74-75 0.45403043686592426 1103 76-77 0.504722434983325 1103 78-79 0.6785852983132195 1103 80-81 0.8348371733518754 1103 82-83 0.9544339086248712 1103 84-85 0.8096147325750849 1103 86-87 0.8405493873376493 1103 88-89 0.7225521403600048 1103 90-91 0.9253074590893355 1103 92-93 1.3616328842684275 1103 94-95 0.9655500193650646 1103 96-97 0.9204361675070558 1103 98-99 1.2211090246851164 1103 100-101 1.5709776889251224 1104 1 0.19519364350350088 1104 2 0.20783481877715104 1104 3 0.19742547491437534 1104 4 0.2038051764306772 1104 5 0.10825929799779743 1104 6 0.012735724187564301 1104 7 0.1590007776135991 1104 8 0.15294938212467457 1104 9 0.20966986653058228 1104 10-11 0.18269089351762346 1104 12-13 0.2642327548622774 1104 14-15 0.3450358841299135 1104 16-17 0.2912505581201472 1104 18-19 0.352285713171014 1104 20-21 0.33434409817242994 1104 22-23 0.37322847247349245 1104 24-25 0.43406656852864955 1104 26-27 0.39744200989795075 1104 28-29 0.43078816005264997 1104 30-31 0.4095437457285769 1104 32-33 0.43686501941976275 1104 34-35 0.39231441385415877 1104 36-37 0.43331488571412535 1104 38-39 0.28572985248749205 1104 40-41 0.3903226963521931 1104 42-43 0.43253230707355783 1104 44-45 0.35214243096901754 1104 46-47 0.35958395907773166 1104 48-49 0.3032039432609892 1104 50-51 0.41836242927434597 1104 52-53 0.36618837045259056 1104 54-55 0.31585785469491867 1104 56-57 0.29488188115531244 1104 58-59 0.5390442555860346 1104 60-61 0.5225419820359747 1104 62-63 0.41652342027753164 1104 64-65 0.5329228231544079 1104 66-67 0.3304476698813552 1104 68-69 0.3937384338325671 1104 70-71 0.4346803260253971 1104 72-73 0.4668171997154218 1104 74-75 0.6109418383954335 1104 76-77 0.6258067657503759 1104 78-79 0.8431726225816938 1104 80-81 0.6531967084109311 1104 82-83 0.7755988734582857 1104 84-85 0.9346309363719101 1104 86-87 0.8957971328369645 1104 88-89 0.8386655684278139 1104 90-91 1.0326547779341944 1104 92-93 1.2360497752221313 1104 94-95 1.282927765753417 1104 96-97 1.3778322674373413 1104 98-99 1.4199084619932663 1104 100-101 1.7018720424875795 1105 1 0.16597015338382448 1105 2 0.235409713890256 1105 3 0.17458874248705314 1105 4 0.15151232908670664 1105 5 0.04658464870045265 1105 6 0.1344100928930061 1105 7 0.1511766526194691 1105 8 0.12917188304032123 1105 9 0.17946399123393775 1105 10-11 0.13805683356359566 1105 12-13 0.23612724321430534 1105 14-15 0.2898815480013468 1105 16-17 0.19594493122570356 1105 18-19 0.20163525332554855 1105 20-21 0.2697407120865094 1105 22-23 0.29732307176121964 1105 24-25 0.2836642914391305 1105 26-27 0.1120222001251463 1105 28-29 0.2038839893204667 1105 30-31 0.3118599848753334 1105 32-33 0.240996703865477 1105 34-35 0.33410186109720286 1105 36-37 0.31462211950667296 1105 38-39 0.17936980589041696 1105 40-41 0.25250868912593916 1105 42-43 0.4635824269795705 1105 44-45 0.3506753585466029 1105 46-47 0.3592143301887418 1105 48-49 0.48464677818603263 1105 50-51 0.5444194005606207 1105 52-53 0.38238040441707 1105 54-55 0.3689638307303298 1105 56-57 0.3926480560833596 1105 58-59 0.5032337137481662 1105 60-61 0.4579389094605659 1105 62-63 0.4271185078970525 1105 64-65 0.3963509445610356 1105 66-67 0.3091567793915928 1105 68-69 0.4411309943692885 1105 70-71 0.5716616493422784 1105 72-73 0.43372440209421015 1105 74-75 0.6160638946983141 1105 76-77 0.4710427737176168 1105 78-79 0.7644137667915913 1105 80-81 0.6794100273875756 1105 82-83 0.6766234885006916 1105 84-85 0.8695273050815899 1105 86-87 0.904480159056714 1105 88-89 0.7552814921124558 1105 90-91 0.7560462985565657 1105 92-93 0.8204293257781679 1105 94-95 1.0680304204585092 1105 96-97 1.1741197622164101 1105 98-99 1.468477470711445 1105 100-101 1.5153595075145034 1106 1 -0.015020803449093734 1106 2 0.0449267771038393 1106 3 0.08456112105862701 1106 4 0.04291767645451472 1106 5 -0.014079066612559643 1106 6 0.009858748700416697 1106 7 0.030714099712163545 1106 8 0.07029478064847439 1106 9 -0.19902418398010013 1106 10-11 0.08862910727866335 1106 12-13 0.008306452348698201 1106 14-15 0.10769434583561122 1106 16-17 0.09793947447791851 1106 18-19 0.1310567394231157 1106 20-21 0.15192874669606482 1106 22-23 0.2445710281901583 1106 24-25 0.23163030919750582 1106 26-27 0.13867194671669125 1106 28-29 0.15267805308503313 1106 30-31 0.13043246623015392 1106 32-33 0.10387286638113125 1106 34-35 0.0742725450644599 1106 36-37 0.17193241341274756 1106 38-39 0.18154680355141295 1106 40-41 0.23586754811875466 1106 42-43 0.1964025534607714 1106 44-45 -0.007043461092031578 1106 46-47 -0.5795465866052112 1106 48-49 -0.2272900421549977 1106 50-51 0.1951013919421527 1106 52-53 0.19974847306572485 1106 54-55 0.3201228202754649 1106 56-57 0.1650486481550999 1106 58-59 0.017466343472769097 1106 60-61 0.09656939173139278 1106 62-63 -0.16060544305095803 1106 64-65 -0.0678012758022355 1106 66-67 0.0499500292459949 1106 68-69 -0.16710531475699497 1106 70-71 -0.10706506029314866 1106 72-73 0.11262243023897867 1106 74-75 0.12564257446125993 1106 76-77 0.14433888686464158 1106 78-79 0.3905757719822738 1106 80-81 -0.06215244767837902 1106 82-83 0.3728580231080514 1106 84-85 0.47457719987953695 1106 86-87 0.5504858972647533 1106 88-89 0.7575707126246272 1106 90-91 0.7434972471969807 1106 92-93 0.7842920755819911 1106 94-95 0.9177154896832782 1106 96-97 1.184803628952519 1106 98-99 1.212938538727446 1106 100-101 1.1880759334844875 1107 1 0.12136085305833433 1107 2 0.18953294421535105 1107 3 0.16806448990640632 1107 4 0.047051837232750415 1107 5 0.04576638795517596 1107 6 0.08866515453566848 1107 7 0.08796704883263828 1107 8 0.09801730964948518 1107 9 0.23948055740660834 1107 10-11 0.20705149020231772 1107 12-13 0.2186446729010001 1107 14-15 0.20907763836042648 1107 16-17 0.23772046260791058 1107 18-19 0.32206697709293763 1107 20-21 0.25782946923977335 1107 22-23 0.27775552683355187 1107 24-25 0.31564853332821485 1107 26-27 0.28466811586250174 1107 28-29 0.2658376070589057 1107 30-31 0.18987412474736942 1107 32-33 0.3301493888576772 1107 34-35 0.34784299062490476 1107 36-37 0.41063577466253776 1107 38-39 0.28336008678707003 1107 40-41 0.42096652410666735 1107 42-43 0.328035317190448 1107 44-45 0.37104613016096977 1107 46-47 0.41612424792297276 1107 48-49 0.553839937281424 1107 50-51 0.6398622147792423 1107 52-53 0.5852157674238541 1107 54-55 0.4496293741488415 1107 56-57 0.5166382810744636 1107 58-59 0.4608602952329264 1107 60-61 0.5763667216529882 1107 62-63 0.7476985400079919 1107 64-65 0.5482208490350828 1107 66-67 0.4596792469233151 1107 68-69 0.773210381451122 1107 70-71 0.9417290049465699 1107 72-73 0.8643630730773921 1107 74-75 0.7450654095874683 1107 76-77 0.8575171600636118 1107 78-79 1.0718809645911485 1107 80-81 1.0604309034244324 1107 82-83 1.1260217559727614 1107 84-85 1.2171169299795608 1107 86-87 1.4541243202665477 1107 88-89 1.4159194257468357 1107 90-91 1.5435699488682921 1107 92-93 1.7706742222818939 1107 94-95 1.7838598562688226 1107 96-97 1.7706868822619306 1107 98-99 1.905257743488196 1107 100-101 1.9754808361445164 1108 1 0.1256988067745013 1108 2 0.18032695186942505 1108 3 0.16444116974602707 1108 4 0.10384622731766768 1108 5 0.05499783528623681 1108 6 0.10373177841371728 1108 7 0.015415416880202315 1108 8 0.0390262149548235 1108 9 0.1212433636126562 1108 10-11 0.1398440793470357 1108 12-13 0.2129226043682948 1108 14-15 0.32081153800656637 1108 16-17 0.3564402783744214 1108 18-19 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-1.119856778403431 2316 60-61 -0.9734975781318695 2316 62-63 -1.1496195327986847 2316 64-65 -1.0339524991865687 2316 66-67 -1.0991746100853845 2316 68-69 -1.005505579635539 2316 70-71 -0.9556892860921273 2316 72-73 -1.1807523012069652 2316 74-75 -1.1158029466530976 2316 76-77 -1.201930367197928 2316 78-79 -1.4089779714993753 2316 80-81 -1.5291353491572295 2316 82-83 -1.5654263876581886 2316 84-85 -1.5639148543691377 2316 86-87 -1.7954789173898433 2316 88-89 -1.7151296448827544 2316 90-91 -1.371339861270723 2316 92-93 -1.5587477989848857 2316 94-95 -1.727268077924581 2316 96-97 -1.910741111502201 2316 98-99 -1.90956071709574 2316 100-101 -2.0409079913887425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 16.0 4 74.0 5 120.0 6 265.0 7 776.0 8 1754.0 9 3067.0 10 5049.0 11 7878.0 12 10949.0 13 15015.0 14 19558.0 15 26096.0 16 32622.0 17 40217.0 18 38802.0 19 34183.0 20 33714.0 21 35224.0 22 38187.0 23 42213.0 24 49514.0 25 57528.0 26 67879.0 27 81299.0 28 96988.0 29 115699.0 30 135797.0 31 161627.0 32 194188.0 33 241000.0 34 303810.0 35 395172.0 36 500684.0 37 491704.0 38 184706.0 39 5731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18126590291364 18.104048402221096 14.976433348505388 23.73825234635987 2 16.625361700851297 19.810376895675855 37.695624173167644 25.868637230305204 3 19.824854213328493 22.551317196629682 29.457382441029544 28.16644614901228 4 14.102180127519775 16.220850944723846 34.372111109797935 35.30485781795845 5 14.623400832740701 36.5984289935419 33.164368526115865 15.61380164760153 6 34.70016805463072 34.790075840777604 15.75781684639576 14.751939258195906 7 30.540945660414344 31.97079366177492 18.802747678799463 18.685512999011276 8 28.327438449631178 33.418773114718185 18.602609891297767 19.651178544352874 9 27.10150442044213 16.09966244944669 18.74867040826033 38.05016272185085 10-11 26.00554609107235 25.549362804869258 26.09376756574453 22.35132353831386 12-13 27.25769721917149 22.629651409151048 26.39959528524607 23.71305608643139 14-15 23.396260135884997 24.223374294847957 24.59875875945127 27.781606809815774 16-17 23.36672806569985 27.363862777484716 25.74008895653352 23.529320200281916 18-19 23.712220137153334 25.374116127767643 28.032996935813507 22.880666799265516 20-21 23.557958669514658 24.641493063062285 28.566130794353594 23.23441747306946 22-23 23.609902828102886 24.485444393518797 28.63691263753528 23.267740140843042 24-25 23.752958096716824 24.956188890384734 28.173880305079262 23.116972707819176 26-27 22.780554956207734 25.39363459461579 28.110445847038463 23.715364602138024 28-29 23.19693511903784 25.9162660592481 27.819666559722716 23.067132261991343 30-31 23.095418500081937 25.280901872400495 27.657114722635118 23.966564904882446 32-33 22.670428643061015 25.2315839403234 27.775832828038755 24.32215458857683 34-35 23.92902127124696 25.32958569458695 27.425978176668714 23.315414857497377 36-37 24.18916440857902 24.58780649971715 27.499240256104336 23.723788835599493 38-39 23.280720550147514 25.106800726038824 27.42354662153115 24.188932102282514 40-41 23.952646180135577 24.907367264366652 27.6546117755852 23.485374779912576 42-43 23.52503526885911 25.089159445222432 27.291366033393427 24.09443925252503 44-45 23.366891139721467 25.265276182000612 27.85796663130852 23.509866046969396 46-47 24.134057438363154 25.780762212786563 26.658826038955237 23.426354309895046 48-49 23.62898553231232 25.85274032821098 26.40110864169773 24.11716549777897 50-51 23.91912514182402 26.07555645748969 26.613781489544223 23.39153691114206 52-53 24.6084010019861 25.32234492420246 26.609144708585198 23.460109365226238 54-55 23.521303108234378 25.88401842055462 26.974861630364078 23.619816840846923 56-57 23.66092455990153 25.963806278843855 26.732202207482608 23.643066953772003 58-59 23.693608091726066 25.339105064508132 26.51527765824218 24.452009185523618 60-61 22.85903300846615 25.956484516201563 27.07066077466555 24.11382170066674 62-63 23.369371249734055 26.02470512466436 27.106001175061706 23.499922450539877 64-65 22.85407612413053 26.05836113186501 26.802402208294886 24.285160535709576 66-67 22.690039547534806 25.993239572557407 27.035540602851626 24.281180277056162 68-69 23.706271468201546 25.382312018271865 27.104337838063806 23.80707867546278 70-71 23.259065912486136 25.864260476217588 26.41124554370252 24.465428067593752 72-73 23.297274587195698 25.988254904179247 26.42179580771135 24.292674700913707 74-75 23.26676563929465 26.558811682614447 26.247030604916127 23.927392073174776 76-77 23.43991289096359 25.806282757997145 26.68655873594417 24.067245615095093 78-79 23.476446276166058 26.214479536369257 26.57658833134829 23.732485856116394 80-81 23.848521973961507 26.22940089782142 26.142481935013045 23.77959519320403 82-83 23.623881882176597 26.40173215174754 26.228482373175925 23.745903592899932 84-85 23.324689957077187 26.63044982459539 26.241512308306174 23.80334791002124 86-87 23.90082067098004 26.777983519697457 25.496584213262025 23.82461159606048 88-89 23.387745334007093 26.918930739921755 25.269635339723866 24.423688586347286 90-91 23.201532660730283 26.81043314187088 25.686094612880332 24.3019395845185 92-93 23.41691054566424 26.3127513875874 26.027679665537374 24.242658401210985 94-95 23.007755277338422 26.01204761783248 26.30091883161491 24.679278273214184 96-97 23.39926007064784 25.503772099798617 26.283107253421406 24.81386057613214 98-99 23.034625931724133 26.102335929451424 25.670824070410138 25.19221406841431 100-101 23.12174641196261 25.73121897770972 26.217585927590715 24.929448682736957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 579.0 1 409.0 2 347.0 3 465.0 4 411.0 5 403.0 6 731.0 7 1679.5 8 2667.5 9 2916.0 10 2470.0 11 2023.0 12 2013.5 13 2199.0 14 2535.5 15 2883.0 16 3153.0 17 3486.5 18 3862.5 19 4323.0 20 5021.0 21 5953.5 22 6980.5 23 8254.0 24 10136.5 25 12506.5 26 15831.0 27 19821.0 28 22961.5 29 25905.0 30 29697.0 31 37193.0 32 51648.5 33 53685.5 34 45704.0 35 45710.0 36 49863.5 37 55758.0 38 61485.5 39 66675.5 40 73224.5 41 82810.0 42 89824.0 43 95737.5 44 102608.0 45 112305.0 46 118774.0 47 125515.5 48 130903.0 49 129326.5 50 130548.5 51 131033.0 52 132024.0 53 140296.5 54 150798.0 55 145762.0 56 134590.0 57 126907.5 58 121615.0 59 110258.0 60 88166.0 61 73439.5 62 64497.5 63 48751.5 64 33677.5 65 25637.0 66 19200.0 67 14770.5 68 11433.5 69 8715.5 70 6839.0 71 4924.0 72 3503.5 73 2927.5 74 2333.0 75 1742.5 76 1273.5 77 868.5 78 556.0 79 368.5 80 236.0 81 140.0 82 80.5 83 45.5 84 25.0 85 14.5 86 8.5 87 8.0 88 6.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7473588326688976E-4 2 0.052203591122795184 3 0.0 4 2.8825837174376134E-5 5 0.04393057585374923 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00694702675902465 26-27 0.0017727889862241325 28-29 8.647751152312841E-5 30-31 0.0017439631490497561 32-33 3.3149712750532557E-4 34-35 0.012596890845202371 36-37 0.02409839987777845 38-39 0.004309462657569233 40-41 9.800784639287887E-4 42-43 0.00126833683567255 44-45 0.009209854977213177 46-47 0.0 48-49 0.0 50-51 6.34168417836275E-4 52-53 0.0 54-55 0.001455704777305995 56-57 0.0 58-59 0.023824554424621877 60-61 0.0 62-63 0.010261998034077905 64-65 0.01589744920166844 66-67 0.007105568863483717 68-69 0.01752610900202069 70-71 7.206459293594034E-5 72-73 7.206459293594034E-5 74-75 0.02337775394841905 76-77 0.049911937067432285 78-79 0.031463401275831554 80-81 0.02700980943239044 82-83 0.03702678785048615 84-85 0.016084817143301886 86-87 0.010463778894298538 88-89 0.08629014358149496 90-91 0.02574147259671789 92-93 0.02479021996996348 94-95 0.0275430874201164 96-97 0.03881398975529747 98-99 0.054250225562175895 100-101 0.015436235806878421 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3469110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.99807852857193 #Duplication Level Percentage of deduplicated Percentage of total 1 67.83460609354621 25.77584689297313 2 15.152211107929544 11.515098151212134 3 6.231629740520326 7.103698687238272 4 3.194222070938102 4.8549720435681465 5 1.8425756099938764 3.5007166361689332 6 1.2153501719495827 2.7708582764072194 7 0.8189667492091364 2.178341399411663 8 0.5841887703469009 1.7758440616921136 9 0.43966223853767444 1.503568823940205 >10 2.3903327184696748 16.55343109831076 >50 0.17652520723673834 4.608419031270164 >100 0.1028766335815238 7.2844402292176635 >500 0.009565152502227189 2.5649132148312264 >1k 0.006300854424837056 5.34351631277969 >5k 9.10966904795719E-4 2.358377095615739 >10k+ 7.591390873297658E-5 0.3079580453630669 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10676 0.30774463767363963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8682 0.2502659183479336 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 8314 0.2396580102677632 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 8002 0.23066434906935784 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 7566 0.21809628406132983 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7279 0.2098232687922839 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7018 0.20229972528977175 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 6668 0.19221068227874008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6134 0.17681768522762323 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 6034 0.1739351015101856 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5731 0.16520087284634966 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5268 0.1518545102346135 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 5062 0.145916387776692 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4944 0.1425149389901156 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4931 0.14214020310684874 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4720 0.13605795146305535 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4463 0.1286497113092407 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 4448 0.12821732375162506 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4342 0.12516178501114117 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4121 0.11879127499560406 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 4084 0.11772471902015214 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3964 0.114265618559227 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3863 0.11135420900461501 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3831 0.11043178221503498 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3701 0.10668442338236608 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3616 0.1042342272225441 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.647751152312841E-5 2 0.0 0.0 0.0 0.0 8.647751152312841E-5 3 0.0 0.0 0.0 0.0 8.647751152312841E-5 4 0.0 0.0 0.0 0.0 8.647751152312841E-5 5 0.0 0.0 0.0 0.0 8.647751152312841E-5 6 0.0 0.0 0.0 0.0 8.647751152312841E-5 7 2.8825837174376137E-5 0.0 0.0 0.0 8.647751152312841E-5 8 2.8825837174376137E-5 0.0 0.0 0.0 8.647751152312841E-5 9 2.8825837174376137E-5 0.0 0.0 2.8825837174376137E-5 8.647751152312841E-5 10-11 2.8825837174376137E-5 0.0 0.0 2.8825837174376137E-5 1.1530334869750455E-4 12-13 5.7651674348752275E-5 0.0 0.0 2.8825837174376137E-5 4.468004762028301E-4 14-15 5.7651674348752275E-5 0.0 0.0 5.7651674348752275E-5 5.044521505515823E-4 16-17 5.7651674348752275E-5 0.0 0.0 8.647751152312841E-5 5.332779877259585E-4 18-19 5.7651674348752275E-5 0.0 0.0 8.647751152312841E-5 6.34168417836275E-4 20-21 5.7651674348752275E-5 0.0 0.0 1.441291858718807E-4 7.927105222953438E-4 22-23 5.7651674348752275E-5 0.0 0.0 3.6032296467970173E-4 8.071234408825318E-4 24-25 8.647751152312841E-5 0.0 0.0 7.062330107722153E-4 8.791880338184722E-4 26-27 8.647751152312841E-5 0.0 0.0 0.0011818593241494217 9.656655453416006E-4 28-29 1.0089043011031648E-4 0.0 0.0 0.0035599908910354527 9.800784639287887E-4 30-31 1.1530334869750455E-4 0.0 0.0 0.01170328989279671 9.94491382515977E-4 32-33 1.1530334869750455E-4 0.0 0.0 0.02768721660598828 0.0010089043011031649 34-35 1.2971626728469263E-4 0.0 0.0 0.05265039159899801 0.001066555975451917 36-37 1.441291858718807E-4 0.0 0.0 0.09414518421151247 0.001066555975451917 38-39 1.441291858718807E-4 0.0 0.0 0.15919068579549223 0.0011242076498006693 40-41 1.441291858718807E-4 0.0 0.0 0.2325524414042795 0.0011242076498006693 42-43 1.441291858718807E-4 0.0 0.0 0.3070239917442802 0.0011386205683878575 44-45 1.7295502304625682E-4 0.0 0.0 0.3998431874457714 0.0011818593241494217 46-47 2.1619377880782102E-4 0.0 0.0 0.5124801462046462 0.0012106851613237978 48-49 2.306066973950091E-4 0.0 0.0 0.6317902862693889 0.0012827497542597381 50-51 2.306066973950091E-4 0.0 0.0 0.7684824061502806 0.0013836401843700546 52-53 2.306066973950091E-4 0.0 0.0 0.8983572155394323 0.001498943533067559 54-55 2.306066973950091E-4 0.0 0.0 1.0370527311039432 0.001498943533067559 56-57 2.594325345693852E-4 0.0 0.0 1.2028445336123674 0.0015277693702419352 58-59 2.594325345693852E-4 0.0 0.0 1.3758716212515603 0.0015565952074163114 60-61 2.594325345693852E-4 0.0 0.0 1.57387629680235 0.0016142468817650637 62-63 2.594325345693852E-4 0.0 0.0 1.767081470463606 0.0017872019048113205 64-65 2.594325345693852E-4 0.0 0.0 1.9643222613292746 0.0018160277419856966 66-67 2.594325345693852E-4 0.0 0.0 2.2011841653911235 0.0018160277419856966 68-69 2.594325345693852E-4 0.0 0.0 2.4660647831864657 0.0018160277419856966 70-71 2.594325345693852E-4 0.0 0.0 2.7412362248530604 0.001888092334921637 72-73 2.594325345693852E-4 0.0 0.0 3.033789646335804 0.002133111950903834 74-75 2.594325345693852E-4 0.0 0.0 3.329701277849362 0.0022772411367757146 76-77 2.594325345693852E-4 0.0 0.0 3.6525938929581363 0.002334892811124467 78-79 2.594325345693852E-4 0.0 0.0 4.018999109281632 0.0023925444854732197 80-81 2.594325345693852E-4 0.0 0.0 4.395046568139955 0.0024357832412347834 82-83 2.594325345693852E-4 0.0 0.0 4.797138170885328 0.0025654995085194763 84-85 2.738454531565733E-4 0.0 0.0 5.212878807532768 0.0025654995085194763 86-87 2.882583717437614E-4 0.0 0.0 5.637094240309474 0.0026663899386297927 88-89 2.882583717437614E-4 0.0 0.0 6.083274961013055 0.002738454531565733 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6215 0.0 54.91123 1 GTATCAA 11055 0.0 41.87724 1 TATCAAC 14725 0.0 31.149412 2 ATCAACG 14725 0.0 30.940113 3 TCAACGC 14855 0.0 30.669348 4 CAACGCA 15430 0.0 29.495667 5 AGGTAAG 2745 0.0 28.74376 2 AACGCAG 16035 0.0 28.457724 6 AAGGTAA 2595 0.0 28.390442 1 TAAGGTG 3155 0.0 28.308516 5 TAAGGTA 1330 0.0 27.14692 4 GTAAGGT 3390 0.0 26.346125 4 GTGTAAG 1505 0.0 25.265701 1 ACGCAGA 18230 0.0 24.90101 7 CGCAGAG 18455 0.0 24.520231 8 GGTAAGG 3475 0.0 24.471287 3 GCAGAGT 20215 0.0 22.009571 9 TACATGG 12285 0.0 21.511837 2 GTACATG 12395 0.0 21.359276 1 TGGTATC 3005 0.0 21.353405 2 >>END_MODULE