##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938622_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3434495 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05590720033076 31.0 31.0 34.0 28.0 34.0 2 31.14425323082433 31.0 31.0 34.0 27.0 34.0 3 31.206077458258054 31.0 31.0 34.0 27.0 34.0 4 34.85011508242114 37.0 35.0 37.0 32.0 37.0 5 34.85733069927311 37.0 35.0 37.0 32.0 37.0 6 34.89766472217895 37.0 35.0 37.0 32.0 37.0 7 34.818593999991265 37.0 35.0 37.0 32.0 37.0 8 34.763414417549015 37.0 35.0 37.0 32.0 37.0 9 36.2701876112791 39.0 35.0 39.0 32.0 39.0 10-11 36.11478310493973 39.0 35.0 39.0 31.0 39.0 12-13 35.96690896332649 38.0 35.0 39.0 30.0 39.0 14-15 36.94308624703196 39.0 36.0 41.0 31.0 41.0 16-17 36.79014673190673 39.0 36.0 41.0 30.0 41.0 18-19 36.77712735060031 39.0 36.0 41.0 30.0 41.0 20-21 36.84315161326484 39.0 36.0 41.0 30.0 41.0 22-23 36.774964587224616 39.0 36.0 41.0 30.0 41.0 24-25 36.63707459175221 39.0 36.0 41.0 30.0 41.0 26-27 36.427420916321026 39.0 35.5 41.0 30.0 41.0 28-29 36.23706221729832 39.0 35.0 40.5 29.0 41.0 30-31 35.983855268387344 38.5 35.0 40.0 28.0 41.0 32-33 35.67483574732239 38.0 35.0 40.0 27.0 41.0 34-35 35.40628855188318 38.0 34.0 40.0 26.5 41.0 36-37 35.08674273801534 38.0 34.0 40.0 25.5 41.0 38-39 34.78812882243241 38.0 33.5 40.0 24.5 41.0 40-41 34.69102677395075 38.0 33.0 40.0 24.0 41.0 42-43 34.46534876306414 38.0 33.0 40.0 24.0 41.0 44-45 34.14538687638212 38.0 33.0 40.0 23.0 41.0 46-47 33.736164851018856 38.0 33.0 40.0 21.5 41.0 48-49 33.31391936805848 37.5 32.0 40.0 19.5 41.0 50-51 32.521459486765885 36.5 31.0 39.5 17.5 40.5 52-53 32.538897130436936 36.5 31.0 39.0 17.0 40.0 54-55 32.618691976549684 37.0 31.0 40.0 15.0 41.0 56-57 32.26587824410867 36.0 31.0 39.0 12.0 41.0 58-59 31.884923693294066 36.0 31.0 39.0 8.5 41.0 60-61 31.389494234232398 35.0 30.0 39.0 7.5 40.0 62-63 30.83172649836439 35.0 29.0 38.5 4.0 40.0 64-65 30.292021243297775 35.0 29.0 38.0 2.0 40.0 66-67 29.727363120342293 34.0 28.0 37.0 2.0 39.5 68-69 29.14128336189163 34.0 27.5 36.5 2.0 39.0 70-71 28.48480912040926 34.0 26.0 36.0 2.0 39.0 72-73 27.929543935862476 33.0 26.0 35.5 2.0 38.5 74-75 27.328641910965075 33.0 25.0 35.0 2.0 37.5 76-77 26.59842990017455 32.5 23.5 35.0 2.0 37.0 78-79 25.821667814336607 32.0 20.5 35.0 2.0 36.0 80-81 25.19404657744443 31.0 19.0 34.5 2.0 36.0 82-83 24.537300389140178 31.0 17.0 34.0 2.0 35.0 84-85 23.897249959601048 30.5 9.0 34.0 2.0 35.0 86-87 23.25629604934641 30.0 4.5 34.0 2.0 35.0 88-89 22.44156622734929 29.5 2.0 34.0 2.0 35.0 90-91 21.60418897101321 29.0 2.0 34.0 2.0 35.0 92-93 20.759343804547683 28.0 2.0 34.0 2.0 35.0 94-95 19.786640539584422 26.5 2.0 33.0 2.0 35.0 96-97 18.55677370326642 25.0 2.0 33.0 2.0 34.5 98-99 17.06583049327485 20.5 2.0 32.0 2.0 34.0 100-101 13.748612678137542 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6783862037007431 1101 2 0.6516923682394129 1101 3 0.6984913133129105 1101 4 0.5340347930935394 1101 5 0.6086832639684019 1101 6 0.5830543987387742 1101 7 0.368655394857619 1101 8 0.48785152506379603 1101 9 0.5995168953800274 1101 10-11 0.570267796964302 1101 12-13 0.6584514474402923 1101 14-15 0.9026231629559547 1101 16-17 0.8482301377726316 1101 18-19 0.7270474029637484 1101 20-21 0.8481475937305447 1101 22-23 0.8270444189751274 1101 24-25 0.9168994709054061 1101 26-27 0.8305393530383824 1101 28-29 0.809367824639672 1101 30-31 0.8130822560912634 1101 32-33 0.8714237011978199 1101 34-35 0.9414950726474913 1101 36-37 0.8207844957603854 1101 38-39 0.8002932968976353 1101 40-41 0.7680028849509597 1101 42-43 0.8036604250878057 1101 44-45 0.6407250359809922 1101 46-47 0.6941197373754733 1101 48-49 0.6785536700776404 1101 50-51 0.6871835242767972 1101 52-53 0.7177622821436316 1101 54-55 0.784097255063422 1101 56-57 0.8417513322711514 1101 58-59 0.9756929131084568 1101 60-61 0.9153450703208996 1101 62-63 0.8186473936106395 1101 64-65 0.7596182511740857 1101 66-67 0.8015723378557347 1101 68-69 0.8323441421225581 1101 70-71 1.11536674887601 1101 72-73 0.9361678820420956 1101 74-75 0.8337983088392882 1101 76-77 1.0363474882994588 1101 78-79 0.797460277079626 1101 80-81 0.9461778126909088 1101 82-83 1.1472678530716856 1101 84-85 1.1852160455082448 1101 86-87 1.2880879833470829 1101 88-89 1.3225725924195828 1101 90-91 1.3246896842130944 1101 92-93 1.2734063966226365 1101 94-95 1.1658450922044317 1101 96-97 1.3342020554716392 1101 98-99 1.4891922351029159 1101 100-101 1.0859583630222556 1102 1 0.6548862454933939 1102 2 0.7014199425667407 1102 3 0.6164737186520846 1102 4 0.5532521457390231 1102 5 0.6089743682085995 1102 6 0.634430290257157 1102 7 0.562527557418278 1102 8 0.5513672275004708 1102 9 0.7373532621829284 1102 10-11 0.8161498227777173 1102 12-13 0.7964171990823488 1102 14-15 0.868115383046046 1102 16-17 0.9057867295458095 1102 18-19 0.9574321348631685 1102 20-21 0.7838369456238539 1102 22-23 0.8810844640720532 1102 24-25 0.6900570042386533 1102 26-27 0.7541371163067154 1102 28-29 0.6596814000026328 1102 30-31 0.6530853926369389 1102 32-33 0.6418919680867319 1102 34-35 0.718184294923006 1102 36-37 0.8407615708355465 1102 38-39 0.8741500488247311 1102 40-41 0.7953439468347483 1102 42-43 1.0038549729215802 1102 44-45 0.8972988993489253 1102 46-47 0.7833440722363036 1102 48-49 0.770131858542662 1102 50-51 0.9916399816891541 1102 52-53 1.0393769769813161 1102 54-55 1.009098561957451 1102 56-57 0.9419359188234608 1102 58-59 1.096264068200579 1102 60-61 1.1961774029445813 1102 62-63 1.043607886273815 1102 64-65 1.0279008134989525 1102 66-67 1.0952946059603157 1102 68-69 0.9194761419611659 1102 70-71 1.0935812679143133 1102 72-73 1.1639933380269802 1102 74-75 1.1078955013140757 1102 76-77 1.1616528146218172 1102 78-79 1.2516892137915718 1102 80-81 1.3368433371803334 1102 82-83 1.4045707350129781 1102 84-85 1.2234007403394038 1102 86-87 1.1252447932061642 1102 88-89 1.5260926148282756 1102 90-91 1.2952647203844734 1102 92-93 1.2875514628856592 1102 94-95 1.2744226873721765 1102 96-97 1.2987741848870407 1102 98-99 1.4093450629653574 1102 100-101 1.131640809110431 1103 1 0.5714720021843114 1103 2 0.6419535476179803 1103 3 0.6174561023628726 1103 4 0.5453627460952575 1103 5 0.6282356965379705 1103 6 0.5675129260315543 1103 7 0.49828627033043205 1103 8 0.5358542666791948 1103 9 0.521952973542831 1103 10-11 0.6115159468871809 1103 12-13 0.5388872004074798 1103 14-15 0.736648377165281 1103 16-17 0.7762212134075668 1103 18-19 0.7970712408660177 1103 20-21 0.7565183196720255 1103 22-23 0.80373124752591 1103 24-25 0.7605095235929582 1103 26-27 0.8449380582685322 1103 28-29 0.6772488543695871 1103 30-31 0.8882716907898143 1103 32-33 0.9530724118356702 1103 34-35 0.8503411268000747 1103 36-37 0.8641346394220548 1103 38-39 0.7924662379089114 1103 40-41 0.7846390515435075 1103 42-43 0.8029061417262042 1103 44-45 0.8795646302160449 1103 46-47 0.7152645561146613 1103 48-49 0.6623823829079143 1103 50-51 0.8102534314365712 1103 52-53 0.8661577607146356 1103 54-55 0.8655726899621499 1103 56-57 0.9242571643448301 1103 58-59 0.9178148750316133 1103 60-61 0.8015512124486115 1103 62-63 0.8700325638104012 1103 64-65 0.7287665081145569 1103 66-67 0.8638673720416001 1103 68-69 1.0221110479870426 1103 70-71 1.0455457916130193 1103 72-73 1.0835930439708186 1103 74-75 0.8758928106135642 1103 76-77 1.0362220570119796 1103 78-79 1.1763817348212449 1103 80-81 1.0017298038449987 1103 82-83 1.0417104358801659 1103 84-85 0.8765450452219135 1103 86-87 1.0630148968226862 1103 88-89 1.1398464742250916 1103 90-91 1.2968509500782943 1103 92-93 1.3953387606994845 1103 94-95 1.1532022875150147 1103 96-97 1.3660883660558234 1103 98-99 1.4097498686026846 1103 100-101 1.1306023691390124 1104 1 0.7083285526842396 1104 2 0.5727888588361836 1104 3 0.6191143238570582 1104 4 0.6376344320794374 1104 5 0.6002416539478617 1104 6 0.6261559214271628 1104 7 0.6389910032763808 1104 8 0.5828681941560632 1104 9 0.6672612378309424 1104 10-11 0.694381232442808 1104 12-13 0.6329931207040502 1104 14-15 0.8690122542740752 1104 16-17 0.9106351019120069 1104 18-19 0.884759707989744 1104 20-21 0.9350333602040308 1104 22-23 1.051169120595624 1104 24-25 1.0299163784702614 1104 26-27 0.9434480860931913 1104 28-29 0.958323680732299 1104 30-31 0.8716895619177905 1104 32-33 0.9302054634107932 1104 34-35 0.7945718311474366 1104 36-37 0.8820453423096595 1104 38-39 0.8957268043586097 1104 40-41 0.9555323497328061 1104 42-43 0.9712497070418493 1104 44-45 0.9737176307138711 1104 46-47 1.1071771070938325 1104 48-49 1.0648865159815202 1104 50-51 0.9091203908783925 1104 52-53 1.0843923960436186 1104 54-55 1.2751306765889652 1104 56-57 1.4338002436757407 1104 58-59 1.2299311326599742 1104 60-61 1.195219760257654 1104 62-63 1.1891699684536263 1104 64-65 1.3388716062720398 1104 66-67 1.249754259749384 1104 68-69 1.224671677302151 1104 70-71 1.1301742177950551 1104 72-73 1.237481782524391 1104 74-75 1.2056568277752042 1104 76-77 1.3269369634127486 1104 78-79 1.550403378975485 1104 80-81 1.5240855835034601 1104 82-83 1.5696554186627516 1104 84-85 1.480739211201744 1104 86-87 1.6407045448245938 1104 88-89 1.7552036399522208 1104 90-91 1.5808172926403792 1104 92-93 1.6924918631039993 1104 94-95 1.3741610332089387 1104 96-97 1.4754793427694572 1104 98-99 1.306405211921497 1104 100-101 0.7860728991708452 1105 1 0.43537617005898 1105 2 0.4540874559601704 1105 3 0.43403881290416635 1105 4 0.45105270303670153 1105 5 0.4316857496900468 1105 6 0.38713174777344506 1105 7 0.37869241395915765 1105 8 0.3546154071402725 1105 9 0.37714275062623415 1105 10-11 0.4554768438687873 1105 12-13 0.43264293841604484 1105 14-15 0.48784295672696487 1105 16-17 0.4902165518628223 1105 18-19 0.6736971777385179 1105 20-21 0.7027583805016206 1105 22-23 0.590336889968107 1105 24-25 0.6076686053505185 1105 26-27 0.6585301254549094 1105 28-29 0.649254754787286 1105 30-31 0.633276267774427 1105 32-33 0.6082961136364062 1105 34-35 0.5954319416912526 1105 36-37 0.6791135597905722 1105 38-39 0.6666313744399588 1105 40-41 0.6350023580860409 1105 42-43 0.8013546982982689 1105 44-45 0.6149454594958641 1105 46-47 0.6132519915536818 1105 48-49 0.6107956725384227 1105 50-51 0.4928887075052444 1105 52-53 0.5712655614533944 1105 54-55 0.6454346623338054 1105 56-57 0.8001269256436743 1105 58-59 0.8549672389569132 1105 60-61 0.8656197692354333 1105 62-63 0.7489456507650587 1105 64-65 0.8165035113573715 1105 66-67 0.7995860727148631 1105 68-69 0.6444947174133198 1105 70-71 0.629279611099971 1105 72-73 0.6045074140918345 1105 74-75 0.6760294154842299 1105 76-77 0.6858857627319992 1105 78-79 0.7875578319553256 1105 80-81 0.9486758746178019 1105 82-83 1.0891169566739016 1105 84-85 0.9476560847477344 1105 86-87 0.932141155829008 1105 88-89 1.0781029853548176 1105 90-91 0.9767945632385349 1105 92-93 0.9803661487848281 1105 94-95 1.0376176549086296 1105 96-97 1.0951526881252107 1105 98-99 1.1573947684190706 1105 100-101 0.9044922825923951 1106 1 0.3302570676327612 1106 2 0.4464617637778652 1106 3 0.12261327521359888 1106 4 0.38835845998173824 1106 5 0.4837164520213548 1106 6 0.2216757553510149 1106 7 0.3724643045872611 1106 8 0.41980120021935363 1106 9 0.4259897206280101 1106 10-11 0.32340200403467634 1106 12-13 0.2722781693025311 1106 14-15 0.5329279787658905 1106 16-17 0.3644089118079421 1106 18-19 0.12527242443526632 1106 20-21 0.39253324071110285 1106 22-23 0.38923999701876966 1106 24-25 0.45979503485044404 1106 26-27 0.28631195197797155 1106 28-29 0.3134285012506126 1106 30-31 0.3728978049285274 1106 32-33 0.5614518217708167 1106 34-35 0.38905860944116455 1106 36-37 0.3271602564657812 1106 38-39 0.4165536772179692 1106 40-41 0.5822725016550336 1106 42-43 0.4029035569550956 1106 44-45 0.6604558012862682 1106 46-47 0.14573809471237098 1106 48-49 0.6001941122750409 1106 50-51 0.29458464281215413 1106 52-53 0.709977146463828 1106 54-55 0.4359409080943166 1106 56-57 0.06989135798325208 1106 58-59 -0.37348371056116747 1106 60-61 0.3194255871131517 1106 62-63 0.463840006722787 1106 64-65 0.5467317848283777 1106 66-67 0.044066950493583334 1106 68-69 0.03329862832394426 1106 70-71 -0.022990864535987754 1106 72-73 0.45555261098497724 1106 74-75 0.5272672434567767 1106 76-77 0.5868093118895175 1106 78-79 0.7209080426727716 1106 80-81 0.5120583103386629 1106 82-83 0.46313586804877716 1106 84-85 0.40065571334856287 1106 86-87 0.4271319014773205 1106 88-89 0.3504245348244126 1106 90-91 0.31890727966237264 1106 92-93 0.37382250325696376 1106 94-95 0.15501968870595206 1106 96-97 0.21943382762428598 1106 98-99 0.2791591707759089 1106 100-101 -0.1607289739490021 1107 1 0.4299512562288932 1107 2 0.46417590031585476 1107 3 0.32147843849034174 1107 4 0.31684213779165304 1107 5 0.33071532113341107 1107 6 0.3699537015005703 1107 7 0.1610019710532029 1107 8 0.20503340518758506 1107 9 0.30159753176204873 1107 10-11 0.3600110732952544 1107 12-13 0.2617724836016251 1107 14-15 0.27706321146445845 1107 16-17 0.34770034415265627 1107 18-19 0.3276221156074257 1107 20-21 0.4391785290984984 1107 22-23 0.4034764611895767 1107 24-25 0.2135348626176352 1107 26-27 0.26187692977153887 1107 28-29 0.3848971089641182 1107 30-31 0.4826180664696693 1107 32-33 0.48525920665553457 1107 34-35 0.5537939366911786 1107 36-37 0.46416469775119396 1107 38-39 0.6536559606024781 1107 40-41 0.5228517992759976 1107 42-43 0.37235450044031637 1107 44-45 0.6133001873764599 1107 46-47 0.6500524157463445 1107 48-49 0.6370853550781064 1107 50-51 0.45004252080130414 1107 52-53 0.4744480697005642 1107 54-55 0.39784062433028566 1107 56-57 0.4551495591145809 1107 58-59 0.5283730955524284 1107 60-61 0.47003907462457306 1107 62-63 0.6121100004595768 1107 64-65 0.5479767487573319 1107 66-67 0.5255904792149586 1107 68-69 0.5101080216042142 1107 70-71 0.4492184692931396 1107 72-73 0.453903993553066 1107 74-75 0.5934771125655445 1107 76-77 0.528315476928956 1107 78-79 0.48475703238728585 1107 80-81 0.41532132240548947 1107 82-83 0.553336795354948 1107 84-85 0.6272205013811138 1107 86-87 0.3443234273862785 1107 88-89 0.5385649097176 1107 90-91 0.5462681316345162 1107 92-93 0.600564163918186 1107 94-95 0.5181882809533924 1107 96-97 0.5300597318684588 1107 98-99 0.3841351325516733 1107 100-101 0.21412472907828928 1108 1 0.4421178654580835 1108 2 0.41081107773008796 1108 3 0.3746087810204308 1108 4 0.44973286467983087 1108 5 0.4156660262629188 1108 6 0.4435601669333451 1108 7 0.38617512815483934 1108 8 0.28066437277257705 1108 9 0.23384968904613856 1108 10-11 0.4431933798492125 1108 12-13 0.5126705354575378 1108 14-15 0.6659284496272733 1108 16-17 0.5285415666924678 1108 18-19 0.5023560449390558 1108 20-21 0.4995328169751332 1108 22-23 0.6147544788532926 1108 24-25 0.5186336212923877 1108 26-27 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-1.1268649069520116 2316 72-73 -0.8668921348916641 2316 74-75 -0.6480933712581134 2316 76-77 -0.5313215922772976 2316 78-79 -0.7417734139093604 2316 80-81 -0.6361808815729049 2316 82-83 -0.4488351997900182 2316 84-85 -0.5495466821073656 2316 86-87 -0.7772008874499576 2316 88-89 -0.9638811020207498 2316 90-91 -1.0049025852503597 2316 92-93 -1.0902984621749177 2316 94-95 -1.047520829358941 2316 96-97 -1.0791609284095038 2316 98-99 -0.8311647602872405 2316 100-101 -0.7467237984006339 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11185.0 3 4051.0 4 4320.0 5 4860.0 6 5863.0 7 7612.0 8 9705.0 9 12348.0 10 15708.0 11 19254.0 12 23962.0 13 27534.0 14 31029.0 15 36436.0 16 41844.0 17 47961.0 18 46270.0 19 44111.0 20 46124.0 21 49233.0 22 54014.0 23 59951.0 24 66681.0 25 74731.0 26 84169.0 27 95650.0 28 109361.0 29 124035.0 30 143850.0 31 167750.0 32 197444.0 33 237106.0 34 289629.0 35 352990.0 36 400197.0 37 349026.0 38 132500.0 39 6001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.376028056779795 17.287047308624288 14.370202827088688 23.966721807507223 2 16.110949270844866 19.051253517598187 38.7251874595804 26.112609751976557 3 19.786618271010816 22.347810761721796 28.858327640726344 29.007243326541044 4 13.55724817227983 15.503291280110588 34.679881312899575 36.25957923471 5 14.700094016972 36.42346269722951 32.98783722798227 15.888606057816212 6 34.664623682417925 34.25276365189553 16.102340341596754 14.98027232408979 7 30.515773750921888 31.862283625754838 18.86878956253867 18.7531530607846 8 28.399401268590573 32.92480992549645 18.75902750127503 19.916761304637955 9 27.29402580530525 16.141350969920026 18.758846087185756 37.80577713758897 10-11 26.00951500838553 25.322680835042842 26.084609732456837 22.583194424114787 12-13 27.273557431552675 22.577727625180668 26.315991072734146 23.832723870532508 14-15 23.549063923718712 24.063781724583524 24.58362587765116 27.8035284740466 16-17 23.457751473430196 27.216028823454884 25.651666101129667 23.674553601985256 18-19 23.86367691216709 25.258885321750967 27.87007300099285 23.00736476508909 20-21 23.649553595813217 24.59740598346932 28.430735796743534 23.322304623973935 22-23 23.63217967572597 24.455602143610484 28.470407522241644 23.441810658421907 24-25 23.633822145431555 24.81406590076716 28.217899671632484 23.3342122821688 26-27 22.701139798259348 25.25312261024726 28.02988966170205 24.015847929791338 28-29 23.12937604795024 25.901659591970848 27.750698245094807 23.2182661149841 30-31 23.032129552414908 25.184456332140115 27.583142454489508 24.200271660955476 32-33 22.587216652936043 25.194030033361337 27.689124296912414 24.529629016790203 34-35 23.871512681199 25.316560236241457 27.363252124602976 23.44867495795656 36-37 24.14039406176377 24.542728864969575 27.39459703525145 23.9222800380152 38-39 23.238170934087155 25.083135917687137 27.259275720001607 24.419417428224097 40-41 23.87214604424903 24.890482685830673 27.570942886445803 23.666428383474493 42-43 23.46820634173838 24.99955223378551 27.22561286817486 24.30662855630125 44-45 23.282933635690757 25.29672316068329 27.72709855093226 23.6932446526937 46-47 24.054738802020506 25.795330670522514 26.603535928945377 23.5463945985116 48-49 23.610546515555658 25.88003622470968 26.301294072426124 24.208123187308537 50-51 23.880861692854523 26.102416768303826 26.476128965942074 23.540592572899577 52-53 24.413091812950555 25.35000173987158 26.59628849720667 23.640617949971194 54-55 23.409672804836767 25.953430471482807 26.936901455616262 23.699995268064164 56-57 23.588885771529664 26.019018544340135 26.682322054397645 23.70977362973256 58-59 23.57880621527495 25.282532351810655 26.55245011822534 24.58621131468906 60-61 22.815036189233197 25.845377604593224 27.12511555060327 24.214470655570313 62-63 23.294843324997462 25.884733215514004 27.221330820682603 23.599092638805924 64-65 22.799888651347686 26.00654930410004 26.92986374349277 24.2636983010595 66-67 22.6635887512051 25.934897895065717 27.06787496589611 24.33363838783307 68-69 23.698255439893458 25.261404474039654 27.224953660827396 23.815386425239492 70-71 23.19988152545586 25.727819005378826 26.52434771288504 24.547951756280273 72-73 23.22954351360344 25.826155267551165 26.622842302912314 24.321458915933086 74-75 23.281846900730326 26.346511578804822 26.522441556285724 23.849199964179128 76-77 23.506083005281397 25.63487641140472 26.757637408709783 24.1014031746041 78-79 23.56121353051319 25.97884912806048 26.664791480949756 23.795145860476573 80-81 23.900039964796036 26.0173879620793 26.224142085405276 23.85842998771938 82-83 23.521175646034315 26.11544597602014 26.40183629905259 23.961542078892954 84-85 23.323430860918183 26.396550707525705 26.419291695390147 23.86072673616596 86-87 23.916900138911316 26.46802877696166 25.670755579420046 23.944315504706985 88-89 23.488838815347318 26.499532751042736 25.567423143480887 24.444205290129055 90-91 23.31982106364486 26.430031550960393 25.84366487473989 24.406482510654858 92-93 23.631583177911953 25.778305815691404 26.261709025545798 24.328401980850845 94-95 23.192209307151735 25.51258218157167 26.57445291775981 24.720755593516785 96-97 23.71898451714436 24.93984812060057 26.537664958000363 24.80350240425471 98-99 23.311863324290137 25.457170236984574 25.972983406114302 25.257983032610987 100-101 23.86455668820337 24.59511549761903 26.456146938556564 25.084180875621044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 552.0 1 406.0 2 362.0 3 477.0 4 415.5 5 421.5 6 749.5 7 1642.0 8 2696.5 9 3025.5 10 2586.0 11 2162.0 12 2162.0 13 2339.5 14 2602.0 15 2919.0 16 3173.5 17 3511.5 18 4037.5 19 4535.5 20 5202.5 21 6090.0 22 7157.5 23 8561.5 24 10395.5 25 12890.5 26 16193.5 27 19876.0 28 22651.5 29 25191.0 30 28795.0 31 36069.5 32 49081.5 33 51373.5 34 45183.5 35 45774.0 36 49714.0 37 55117.0 38 60870.5 39 65905.5 40 72231.0 41 81263.5 42 87721.0 43 93251.0 44 99379.5 45 109137.5 46 116158.0 47 121668.0 48 126959.5 49 126674.5 50 127453.0 51 127782.0 52 128976.0 53 136087.0 54 145031.0 55 142305.0 56 133083.0 57 126060.5 58 122256.5 59 111009.5 60 89730.5 61 75227.5 62 65800.5 63 51048.0 64 36768.0 65 28563.5 66 21776.0 67 16364.0 68 12580.0 69 9666.5 70 7540.0 71 5652.5 72 4142.0 73 3362.0 74 2659.0 75 2015.0 76 1481.5 77 1050.5 78 727.5 79 458.5 80 278.0 81 176.0 82 113.0 83 60.5 84 37.5 85 33.5 86 29.0 87 18.5 88 12.5 89 11.0 90 7.5 91 3.5 92 2.5 93 4.0 94 4.0 95 3.0 96 2.5 97 2.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18354954658545142 2 0.05109921545962361 3 0.04891548830322944 4 0.056485742445395906 5 0.062192549414106006 6 0.06583209467476295 7 0.038520947038793184 8 0.035987823537375944 9 0.04309221588617832 10-11 0.04360175222267029 12-13 0.04125788507480721 14-15 0.036526476235953176 16-17 0.034357307260601634 18-19 0.042495330463430576 20-21 0.052045497227394426 22-23 0.04916297738095411 24-25 0.06886019633162954 26-27 0.05444759709942801 28-29 0.04667352842266476 30-31 0.055539460677625094 32-33 0.05179800814966974 34-35 0.05054600458000376 36-37 0.04826037015631119 38-39 0.02798082396393065 40-41 0.026364865868198965 42-43 0.022812669693797777 44-45 0.004338337950703087 46-47 0.011777568463485898 48-49 0.010918635781970857 50-51 0.008778583168704569 52-53 0.00971030675543275 54-55 0.011137008497610274 56-57 0.009331794048324427 58-59 0.010918635781970857 60-61 0.011457288480548087 62-63 0.004090848872978415 64-65 0.006900577814205582 66-67 0.003959825243594764 68-69 0.008895048617045593 70-71 0.024632442324126255 72-73 0.02074540798574463 74-75 0.021618898848302297 76-77 0.013408084740260213 78-79 0.004906107011365572 80-81 0.006478390563969375 82-83 0.005706806968710102 84-85 0.0048769906492803165 86-87 0.008429186823681501 88-89 0.032042556474823805 90-91 0.038404481590452164 92-93 0.023846300547824353 94-95 0.03399335273453594 96-97 0.03815699251272749 98-99 0.023016484228394567 100-101 0.12755878229550485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3434495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.83671658216276 #Duplication Level Percentage of deduplicated Percentage of total 1 70.97610484691721 28.984310777394267 2 13.6654947793267 11.161078745167787 3 5.652000691434829 6.924274510749361 4 2.879541272376003 4.703640433066367 5 1.6760050204638186 3.4221271005481433 6 1.0635097325609717 2.6058147318578464 7 0.7484549328834248 2.139510937807745 8 0.5515458622765085 1.8018657647880278 9 0.41308385615173115 1.518208952250162 >10 2.1190683781564283 15.742325856670561 >50 0.15294741891896574 4.3007283847785125 >100 0.08800117625356998 6.814267224099324 >500 0.007912240156064121 2.289762428939175 >1k 0.005466638652439702 5.064248467720353 >5k 7.912240154846938E-4 2.202258390671348 >10k+ 7.192945595315398E-5 0.32557729349109316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11084 0.32272575735297326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8270 0.24079231444506397 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 8021 0.23354234028583531 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7975 0.2322029876299136 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7589 0.22096407186500489 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7021 0.2044259782005797 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6503 0.18934370264041728 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 6163 0.17944413953143037 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 6094 0.17743511054754774 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5896 0.17167007085466712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.17158272176841136 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5549 0.1615666932110834 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4872 0.14185491607936537 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4778 0.13911797804335135 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4521 0.13163507298744065 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4439 0.1292475312964497 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4290 0.12490919334574661 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4145 0.12068732084338454 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4109 0.11963913180831534 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 4022 0.1171060083068981 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3750 0.10918635781970856 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3715 0.10816728514672462 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3684 0.1072646779220817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.823272417051124E-5 2 2.911636208525562E-5 0.0 0.0 0.0 5.823272417051124E-5 3 2.911636208525562E-5 0.0 0.0 0.0 5.823272417051124E-5 4 2.911636208525562E-5 0.0 0.0 0.0 5.823272417051124E-5 5 2.911636208525562E-5 0.0 0.0 0.0 8.734908625576686E-5 6 2.911636208525562E-5 0.0 0.0 0.0 1.1646544834102247E-4 7 2.911636208525562E-5 0.0 0.0 0.0 1.1646544834102247E-4 8 2.911636208525562E-5 0.0 0.0 0.0 1.1646544834102247E-4 9 2.911636208525562E-5 0.0 0.0 0.0 1.1646544834102247E-4 10-11 2.911636208525562E-5 0.0 0.0 0.0 1.1646544834102247E-4 12-13 2.911636208525562E-5 0.0 0.0 0.0 2.3293089668204497E-4 14-15 2.911636208525562E-5 0.0 0.0 2.911636208525562E-5 3.3483816398043964E-4 16-17 2.911636208525562E-5 0.0 0.0 2.911636208525562E-5 3.785127071083231E-4 18-19 2.911636208525562E-5 0.0 0.0 2.911636208525562E-5 4.658617933640899E-4 20-21 2.911636208525562E-5 0.0 0.0 7.279090521313905E-5 4.949781554493455E-4 22-23 2.911636208525562E-5 0.0 0.0 1.0190726729839467E-4 5.240945175346012E-4 24-25 2.911636208525562E-5 0.0 0.0 2.911636208525562E-4 5.677690606624847E-4 26-27 2.911636208525562E-5 0.0 0.0 8.152581383871573E-4 6.84234509003507E-4 28-29 5.823272417051124E-5 0.0 0.0 0.00305721801895184 7.133508710887627E-4 30-31 8.734908625576686E-5 0.0 0.0 0.01129714848907918 7.279090521313905E-4 32-33 1.0190726729839467E-4 0.0 0.0 0.03017910930136745 7.570254142166462E-4 34-35 1.3102362938365028E-4 0.0 0.0 0.05635471881601225 7.570254142166462E-4 36-37 1.7469817251153372E-4 0.0 0.0 0.09898107290882649 7.715835952592739E-4 38-39 1.7469817251153372E-4 0.0 0.0 0.16581768207553077 7.861417763019017E-4 40-41 1.8925635355416154E-4 0.0 0.0 0.23895798363369286 7.861417763019017E-4 42-43 2.3293089668204495E-4 2.911636208525562E-5 0.0 0.31749937035866993 8.006999573445296E-4 44-45 2.911636208525562E-4 2.911636208525562E-5 0.0 0.4151410906115746 8.734908625576685E-4 46-47 2.911636208525562E-4 2.911636208525562E-5 0.0 0.5288841590976257 9.608399488134354E-4 48-49 2.911636208525562E-4 2.911636208525562E-5 0.0 0.6491638508718167 0.001004514491941319 50-51 2.911636208525562E-4 2.911636208525562E-5 0.0 0.7887476907085321 0.001077305397154458 52-53 2.911636208525562E-4 2.911636208525562E-5 0.0 0.9181553619964506 0.0011937708454954803 54-55 2.911636208525562E-4 2.911636208525562E-5 0.0 1.0567346873412249 0.0012520035696659917 56-57 3.202799829378118E-4 2.911636208525562E-5 0.0 1.2213877149333454 0.0013102362938365029 58-59 3.202799829378118E-4 2.911636208525562E-5 0.0 1.3986190109462964 0.0013539108369643863 60-61 3.202799829378118E-4 2.911636208525562E-5 0.0 1.5953582695563686 0.0014267017421775254 62-63 3.202799829378118E-4 2.911636208525562E-5 0.0 1.787744632034695 0.0015140508284332923 64-65 3.202799829378118E-4 2.911636208525562E-5 0.0 1.9907584666741398 0.00152860900947592 66-67 3.4939634502306744E-4 2.911636208525562E-5 0.0 2.2308665466102005 0.0015868417336464312 68-69 3.4939634502306744E-4 2.911636208525562E-5 0.0 2.4932777599035667 0.0016013999146890592 70-71 3.4939634502306744E-4 2.911636208525562E-5 0.0 2.765151790874641 0.0016596326388595704 72-73 3.6395452606569523E-4 2.911636208525562E-5 0.0 3.0609303551177103 0.0017906562682432204 74-75 3.785127071083231E-4 2.911636208525562E-5 0.0 3.3539283067816372 0.0018634471734563596 76-77 3.785127071083231E-4 4.367454312788343E-5 0.0 3.668967344544103 0.0018780053544989873 78-79 3.785127071083231E-4 5.823272417051124E-5 0.0 4.0337080123861 0.001979912621797382 80-81 3.785127071083231E-4 5.823272417051124E-5 0.0 4.4070816815863765 0.002038145345967893 82-83 3.785127071083231E-4 5.823272417051124E-5 0.0 4.801826760557229 0.0021400526132662884 84-85 3.9307088815095087E-4 5.823272417051124E-5 0.0 5.20974699337166 0.002154610794308916 86-87 4.0762906919357867E-4 5.823272417051124E-5 0.0 5.627770603829675 0.0022565180616073107 88-89 4.0762906919357867E-4 5.823272417051124E-5 0.0 6.065811713221303 0.002300192604735194 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5880 0.0 52.87019 1 GTATCAA 11440 0.0 45.11057 1 TATCAAC 14890 0.0 34.284706 2 ATCAACG 15190 0.0 33.764046 3 TCAACGC 15395 0.0 33.376198 4 CAACGCA 15855 0.0 32.316032 5 AACGCAG 16435 0.0 31.283964 6 GTGTAAG 1260 0.0 29.093449 1 AGGTAAG 2535 0.0 29.063276 2 TAAGGTG 2775 0.0 28.774765 5 ACGCAGA 18470 0.0 27.678741 7 AAGGTAA 2465 0.0 27.618122 1 CGCAGAG 18625 0.0 27.295336 8 GTAAGGT 2915 0.0 26.416016 4 GGTAAGG 3080 0.0 25.1552 3 TAAGGTA 1375 0.0 24.544064 4 GCAGAGT 20085 0.0 24.43276 9 TGGTATC 2930 0.0 24.171825 2 TACATGG 12330 0.0 23.824085 2 GTACATG 12555 0.0 23.813213 1 >>END_MODULE