FastQCFastQC Report
Thu 26 May 2016
SRR938622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434495
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.30720673636153206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87620.2551175645910097No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA82340.2397441254099948No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA79370.23109656587067384No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC73320.21348116680909418No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA72970.21246209413611025No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA69570.20256253102712332No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG65450.190566589847998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61970.18043409584232908No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA59520.17330058713144145No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA56380.16415804943667117No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA53650.15620928258739641No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA50220.14622237039215372No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG48820.14214607970021795No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT48800.14208784697604743No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG46400.13509992007558608No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT45550.13262502929833936No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT44530.12965516036564328No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG43730.12732585139882283No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA43420.1264232441741799No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG41360.12042527358461724No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT40390.11760098646234746No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT38280.11145743406235853No Hit
TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA37820.11011808140643675No Hit
GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG37760.10994338323392522No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA34420.10021851829744985No Hit
GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG34390.10013116921119408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.053.1749761
GTATCAA108300.041.2409821
TATCAAC143200.031.1225932
ATCAACG145450.030.4298573
TCAACGC145850.030.3459634
AAGGTAA23800.029.7783851
AGGTAAG24800.029.7275562
CAACGCA150500.029.3135975
TAAGGTA11700.028.4426354
AACGCAG158100.028.0887586
TAAGGTG29500.027.5569885
GTGTAAG13350.027.0784761
ACGCAGA178650.024.8045547
CGCAGAG181400.024.2714238
GTAAGGT32000.024.2156034
GGTAAGG34000.022.5118373
GTACATG124700.022.1616211
TACATGG122300.022.0902732
GCAGAGT199800.021.7985029
GGACCGA16650.021.3943866
Produced by FastQC (version 0.11.5)