##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938622_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3434495 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79407773195186 31.0 31.0 34.0 30.0 34.0 2 32.022449588658596 31.0 31.0 34.0 30.0 34.0 3 32.08190898516376 33.0 31.0 34.0 30.0 34.0 4 35.76493079768641 37.0 35.0 37.0 35.0 37.0 5 35.626732896684956 37.0 35.0 37.0 33.0 37.0 6 35.66157499137428 37.0 35.0 37.0 33.0 37.0 7 35.59392137708746 37.0 35.0 37.0 33.0 37.0 8 35.57584244554148 37.0 35.0 37.0 33.0 37.0 9 37.15612426281011 39.0 37.0 39.0 33.0 39.0 10-11 37.066262434506385 39.0 37.0 39.0 33.0 39.0 12-13 36.95986586091988 39.0 37.0 39.0 33.0 39.0 14-15 38.0135747759132 40.0 37.0 41.0 33.0 41.0 16-17 37.94096395539955 40.0 37.0 41.0 33.0 41.0 18-19 38.04645224407082 40.0 37.0 41.0 33.0 41.0 20-21 38.07256860178862 40.0 37.0 41.0 33.0 41.0 22-23 37.98796941034999 40.0 37.0 41.0 33.0 41.0 24-25 37.878830221036864 40.0 37.0 41.0 32.5 41.0 26-27 37.68251547898599 40.0 37.0 41.0 32.0 41.0 28-29 37.55828629827675 39.5 36.5 41.0 32.0 41.0 30-31 37.29545144191504 39.0 36.0 41.0 31.0 41.0 32-33 37.056928747894524 39.0 36.0 41.0 31.0 41.0 34-35 36.895176729038766 39.0 35.0 40.5 30.5 41.0 36-37 36.87239754316137 39.0 35.0 41.0 30.5 41.0 38-39 36.72792462938511 39.0 35.0 41.0 30.0 41.0 40-41 36.52501037270399 39.0 35.0 40.5 30.0 41.0 42-43 36.277506591216465 39.0 35.0 40.0 30.0 41.0 44-45 35.99435084342822 38.0 35.0 40.0 29.0 41.0 46-47 35.602689769529434 38.0 34.5 40.0 27.5 41.0 48-49 35.20162993394953 38.0 34.0 40.0 27.0 41.0 50-51 34.79413261629439 38.0 34.0 40.0 26.0 41.0 52-53 34.25954485302788 37.5 33.0 40.0 24.0 41.0 54-55 33.908655712120705 37.0 33.0 40.0 23.5 41.0 56-57 33.52855980282399 37.0 32.0 39.5 23.0 41.0 58-59 33.049987989500636 36.0 32.0 39.0 22.0 40.5 60-61 32.64502728931036 35.5 31.0 39.0 21.0 40.0 62-63 32.16343829296592 35.0 31.0 38.5 19.5 40.0 64-65 31.634459505691524 35.0 30.5 38.0 18.0 40.0 66-67 31.124000180521445 34.5 30.0 37.5 15.5 39.5 68-69 30.577580692357976 34.0 29.0 37.0 12.0 39.0 70-71 29.983366841413368 34.0 29.0 36.0 8.0 39.0 72-73 29.55288696009166 34.0 28.0 36.0 7.0 38.5 74-75 29.062504094488418 33.5 28.0 35.0 6.5 37.5 76-77 27.856017842506688 31.5 26.0 34.0 2.0 36.0 78-79 28.550239118123628 33.0 27.5 35.0 2.0 36.0 80-81 28.317377663965154 33.0 27.0 35.0 2.0 36.0 82-83 27.875052518638114 33.0 26.5 35.0 2.0 36.0 84-85 27.381787569933863 33.0 26.0 35.0 2.0 35.0 86-87 26.852145075185724 32.0 25.0 34.5 2.0 35.0 88-89 26.21911037867285 32.0 24.0 34.0 2.0 35.0 90-91 25.700893726734208 31.5 23.0 34.0 2.0 35.0 92-93 24.989651171424036 31.0 19.5 34.0 2.0 35.0 94-95 24.24015845124247 31.0 16.0 34.0 2.0 35.0 96-97 23.25742328930454 30.5 2.0 34.0 2.0 35.0 98-99 22.019632726208656 30.0 2.0 34.0 2.0 35.0 100-101 18.38304102932163 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2774655196728695 1101 2 0.28765593924664756 1101 3 0.29301033451440617 1101 4 0.21729368001673066 1101 5 0.12450660888746512 1101 6 0.17257701011592985 1101 7 0.26868591052731006 1101 8 0.23745986826433096 1101 9 0.23185444431457825 1101 10-11 0.3567943755899847 1101 12-13 0.3304859304113492 1101 14-15 0.47157168612073264 1101 16-17 0.4295075351567661 1101 18-19 0.414187333481685 1101 20-21 0.391535956799423 1101 22-23 0.4325066517555598 1101 24-25 0.45702902297212233 1101 26-27 0.4835544147943054 1101 28-29 0.4896471117766623 1101 30-31 0.3396369511387647 1101 32-33 0.30958888086682634 1101 34-35 0.26918317020292193 1101 36-37 0.4759160917973446 1101 38-39 0.3919777265249067 1101 40-41 0.5364798175471961 1101 42-43 0.515304883492341 1101 44-45 0.5123225224562376 1101 46-47 0.5265795853399595 1101 48-49 0.4255197565313509 1101 50-51 0.47013650171263777 1101 52-53 0.6093052975194126 1101 54-55 0.5421898104850484 1101 56-57 0.5183270234902153 1101 58-59 0.6066770469356584 1101 60-61 0.5327847895823794 1101 62-63 0.5241477195975435 1101 64-65 0.5148716969648177 1101 66-67 0.3369702700965469 1101 68-69 0.34450191702351773 1101 70-71 0.27273821736716286 1101 72-73 0.22828084128018133 1101 74-75 0.5230702411508723 1101 76-77 0.49022190972348767 1101 78-79 0.3397412630767853 1101 80-81 0.46998887271043444 1101 82-83 0.5260529415536013 1101 84-85 0.444950893719966 1101 86-87 0.26029505691638377 1101 88-89 0.44728339941525874 1101 90-91 0.5700440991651305 1101 92-93 0.6704197470278643 1101 94-95 0.6724412511388991 1101 96-97 0.9567590022859846 1101 98-99 0.7363914946833283 1101 100-101 0.30543568001395016 1102 1 0.352697142762576 1102 2 0.31345845372009506 1102 3 0.31704674826298884 1102 4 0.2409274492640634 1102 5 0.22815239130977716 1102 6 0.2220018323061197 1102 7 0.28649806088656504 1102 8 0.27004117920438375 1102 9 0.36589024212237575 1102 10-11 0.43556733208062326 1102 12-13 0.3400298444271286 1102 14-15 0.4694580587429229 1102 16-17 0.5246599040920614 1102 18-19 0.5763920790958039 1102 20-21 0.46293840365915173 1102 22-23 0.4291460005318868 1102 24-25 0.4426643239644932 1102 26-27 0.39705689919753695 1102 28-29 0.4132205542361973 1102 30-31 0.38515059590455536 1102 32-33 0.4821187416483639 1102 34-35 0.3711367461585269 1102 36-37 0.4880352626422635 1102 38-39 0.4688804035004921 1102 40-41 0.5353685615880224 1102 42-43 0.4089048859523814 1102 44-45 0.5011534006769125 1102 46-47 0.3948881830440598 1102 48-49 0.6557163515994873 1102 50-51 0.6759658827592361 1102 52-53 0.5098034175022548 1102 54-55 0.6202568578298369 1102 56-57 0.5701391054530163 1102 58-59 0.5564435811486561 1102 60-61 0.6894001287817204 1102 62-63 0.6176097923212396 1102 64-65 0.5572419004889895 1102 66-67 0.594719546039979 1102 68-69 0.6000535143648555 1102 70-71 0.5788448556340846 1102 72-73 0.5275457378454576 1102 74-75 0.40930322834908495 1102 76-77 0.3840342300488544 1102 78-79 0.42402987149881355 1102 80-81 0.5359655428115211 1102 82-83 0.475268343875225 1102 84-85 0.33610893788338814 1102 86-87 0.3046737771964274 1102 88-89 0.36676083562182527 1102 90-91 0.45042662728245375 1102 92-93 0.7203540948215803 1102 94-95 0.6851036798968693 1102 96-97 0.5446681498080927 1102 98-99 0.1651998747581196 1102 100-101 -0.06058298774432558 1103 1 0.32225489226528836 1103 2 0.3209591605164235 1103 3 0.27746290416474295 1103 4 0.16459155004039872 1103 5 0.17143239206080807 1103 6 0.21283857444985443 1103 7 0.2300314431570456 1103 8 0.26161270067419906 1103 9 0.4174770766099911 1103 10-11 0.40058520148054555 1103 12-13 0.3513617845081356 1103 14-15 0.4881384690927959 1103 16-17 0.4306564435883473 1103 18-19 0.5095596568045906 1103 20-21 0.46187829943242065 1103 22-23 0.446125134965456 1103 24-25 0.38103750628833666 1103 26-27 0.41178462103138713 1103 28-29 0.4710992752074432 1103 30-31 0.531920960936425 1103 32-33 0.5347659511659018 1103 34-35 0.5180664788685618 1103 36-37 0.5338008665929621 1103 38-39 0.5359279789273046 1103 40-41 0.5762136264164965 1103 42-43 0.5081906335081996 1103 44-45 0.7263494982946028 1103 46-47 0.6962464596381039 1103 48-49 0.7305244694848128 1103 50-51 0.6559171490169078 1103 52-53 0.8963115601931477 1103 54-55 0.8816172773797959 1103 56-57 0.840218124018925 1103 58-59 0.9715687608265711 1103 60-61 0.8725116050960082 1103 62-63 0.7476544044389968 1103 64-65 0.9047449055017296 1103 66-67 0.8594058349855374 1103 68-69 0.7706071696843857 1103 70-71 0.6114038392772088 1103 72-73 0.7157314340931009 1103 74-75 0.7937811143799394 1103 76-77 0.5542107016565865 1103 78-79 0.6967278728629402 1103 80-81 0.5895231023909311 1103 82-83 0.7048986180833126 1103 84-85 0.6338056845260596 1103 86-87 0.7049874665866085 1103 88-89 0.672703404042057 1103 90-91 0.6373562931943617 1103 92-93 0.7030031476061787 1103 94-95 0.8276937011964378 1103 96-97 0.7743230671066001 1103 98-99 0.5794017960887068 1103 100-101 0.10624676262207089 1104 1 0.3143092691231111 1104 2 0.3336451660427926 1104 3 0.34046051693172785 1104 4 0.29132129556975883 1104 5 0.20573380450127132 1104 6 0.18309077284564523 1104 7 0.2414436150846413 1104 8 0.37561839780889983 1104 9 0.36082849149483565 1104 10-11 0.4581821901379186 1104 12-13 0.4352891718616547 1104 14-15 0.5473101915017367 1104 16-17 0.5318597687760303 1104 18-19 0.5989585037441927 1104 20-21 0.530955885538404 1104 22-23 0.5749056665190011 1104 24-25 0.5881978798727516 1104 26-27 0.643612351906846 1104 28-29 0.6292979608865963 1104 30-31 0.5805309674846129 1104 32-33 0.6725348419460317 1104 34-35 0.647385291187426 1104 36-37 0.8066996608578236 1104 38-39 0.8079428359584213 1104 40-41 0.7346687141423232 1104 42-43 0.7264442902713171 1104 44-45 0.7322970101533102 1104 46-47 0.5800659498430107 1104 48-49 0.5601411350842085 1104 50-51 0.7870569408931445 1104 52-53 0.7959046392142142 1104 54-55 0.8117684194576924 1104 56-57 0.740156515229053 1104 58-59 0.8689522549727968 1104 60-61 0.9665156187046833 1104 62-63 0.9434103523811999 1104 64-65 0.8733830362301092 1104 66-67 0.786021142058619 1104 68-69 0.6462321227763219 1104 70-71 0.4795125716475255 1104 72-73 0.411555205067458 1104 74-75 0.47279019578066084 1104 76-77 0.4931543940801646 1104 78-79 0.5711707428031509 1104 80-81 0.6589055478622434 1104 82-83 0.6704808392222041 1104 84-85 0.7152477192445694 1104 86-87 0.7324658647787317 1104 88-89 0.6201149913833177 1104 90-91 0.6243544113634272 1104 92-93 0.675918432851951 1104 94-95 0.8260057856082312 1104 96-97 0.7306653468831499 1104 98-99 0.7826119083995202 1104 100-101 0.5426798839481783 1105 1 0.18117169046993098 1105 2 0.2408451519906123 1105 3 0.22057004587141193 1105 4 0.166086116716734 1105 5 0.15577098422865987 1105 6 0.16020962696840257 1105 7 0.18786479796596467 1105 8 0.15848927778766608 1105 9 0.17986177954892213 1105 10-11 0.2336969000384599 1105 12-13 0.25622828688656085 1105 14-15 0.38504239402487883 1105 16-17 0.23320116649958322 1105 18-19 0.2562948641513856 1105 20-21 0.2183182912717001 1105 22-23 0.24261843995100918 1105 24-25 0.30532699989397827 1105 26-27 0.2656967684290876 1105 28-29 0.298701143315121 1105 30-31 0.2551234542521499 1105 32-33 0.3364066860605135 1105 34-35 0.36395329983731983 1105 36-37 0.23511721944760922 1105 38-39 0.2879550730655396 1105 40-41 0.31362708650949145 1105 42-43 0.29932997383183135 1105 44-45 0.3215249404232665 1105 46-47 0.12570284540408494 1105 48-49 0.037749863830285335 1105 50-51 0.041469715886513825 1105 52-53 0.1391157283362645 1105 54-55 -0.00180232741569597 1105 56-57 0.05960819719698662 1105 58-59 0.1553593563983071 1105 60-61 0.016245699738924202 1105 62-63 0.22893943649130932 1105 64-65 0.10778316426108958 1105 66-67 0.003920936350311877 1105 68-69 0.06551161674169137 1105 70-71 0.12211243971316321 1105 72-73 0.08089007828556305 1105 74-75 0.08772385777887948 1105 76-77 0.038928639946483656 1105 78-79 0.08763960551334549 1105 80-81 0.2246199841356109 1105 82-83 0.20855785688111084 1105 84-85 0.29628830272232776 1105 86-87 0.3989056472822057 1105 88-89 0.25654409813304824 1105 90-91 0.29444702889430374 1105 92-93 0.3095910189996225 1105 94-95 0.13552628422642954 1105 96-97 -0.005965564791086564 1105 98-99 -0.2959258744006128 1105 100-101 -0.7328723960180952 1106 1 0.1323992743377964 1106 2 0.1629501406036198 1106 3 0.22622130978921007 1106 4 0.12708839798454363 1106 5 -0.05564452697392852 1106 6 -0.003646157315742471 1106 7 0.10289772740269854 1106 8 0.07233716339156615 1106 9 0.049887222971157996 1106 10-11 0.15224325110609982 1106 12-13 0.10112480450352024 1106 14-15 0.23439187322781407 1106 16-17 -0.04030804626313511 1106 18-19 0.15076885879447133 1106 20-21 0.15570013346315648 1106 22-23 0.258990308527288 1106 24-25 0.10421410808720566 1106 26-27 0.10254755333222931 1106 28-29 0.2391074949501828 1106 30-31 0.28403552549888644 1106 32-33 0.3402393191516637 1106 34-35 0.19277205381889928 1106 36-37 -0.03830490633549033 1106 38-39 0.11965071982882591 1106 40-41 0.30792718122916796 1106 42-43 0.42459324974015544 1106 44-45 0.3211208987491858 1106 46-47 -0.03253649363435329 1106 48-49 0.14151007085953182 1106 50-51 0.2721585710743639 1106 52-53 0.12455132765587962 1106 54-55 -0.03062876246082169 1106 56-57 0.13402508921783607 1106 58-59 0.17342424260573353 1106 60-61 -0.16438148126400876 1106 62-63 -0.13814584410850017 1106 64-65 -0.4150962496503894 1106 66-67 -0.18480501136236072 1106 68-69 -0.8426418039994026 1106 70-71 -0.5005349625559816 1106 72-73 -0.17322291765483655 1106 74-75 0.1014906932516304 1106 76-77 0.017876198835175217 1106 78-79 -0.05969600844300871 1106 80-81 -0.6785313958444306 1106 82-83 -0.2779772306685082 1106 84-85 -0.08119583221421323 1106 86-87 -0.01184015255427795 1106 88-89 0.058472601213857445 1106 90-91 -0.31617668797314735 1106 92-93 -0.33703141666965664 1106 94-95 -0.13709049856695543 1106 96-97 -0.4089007788485475 1106 98-99 -0.49746396506069956 1106 100-101 -1.0252261269677696 1107 1 0.11230325306701161 1107 2 0.14190970176206008 1107 3 0.14129557435555995 1107 4 0.10568489036722895 1107 5 0.0946231676929088 1107 6 0.17906299388211266 1107 7 0.023144350917931433 1107 8 0.15480532906061484 1107 9 0.1775152211678801 1107 10-11 0.20873143272385164 1107 12-13 0.20122100127581888 1107 14-15 0.30386908709726157 1107 16-17 0.21622883702725204 1107 18-19 0.18064031489769405 1107 20-21 0.2191762921297027 1107 22-23 0.17634527022956092 1107 24-25 0.2252671062564957 1107 26-27 0.286863640350262 1107 28-29 0.2778574085705259 1107 30-31 0.24016206429076448 1107 32-33 0.38541258851468996 1107 34-35 0.2995288076111038 1107 36-37 0.359192880699986 1107 38-39 0.27921834988917027 1107 40-41 0.32150003026605134 1107 42-43 0.3262888138294642 1107 44-45 0.38548992141411276 1107 46-47 0.36946153636105095 1107 48-49 0.32635019445647373 1107 50-51 0.20349689084472544 1107 52-53 0.39589999104724427 1107 54-55 0.4661708027341618 1107 56-57 0.23345438020265163 1107 58-59 0.18411694711710425 1107 60-61 0.19271558095018548 1107 62-63 0.32931397975378474 1107 64-65 0.2630720738603429 1107 66-67 0.29488562332792867 1107 68-69 0.38249346928389016 1107 70-71 0.20824768209840627 1107 72-73 0.2946929359461805 1107 74-75 0.25840084634380034 1107 76-77 0.10469214051602194 1107 78-79 0.17587639525875787 1107 80-81 0.3213734494235574 1107 82-83 0.27869988930762446 1107 84-85 0.48154760759370063 1107 86-87 0.5172991331325889 1107 88-89 0.41103048106770856 1107 90-91 0.33148645440356006 1107 92-93 0.2873262203339628 1107 94-95 0.010193670208472838 1107 96-97 0.24324670166255657 1107 98-99 0.015581830964851662 1107 100-101 -0.21862962800377517 1108 1 0.16584849690599768 1108 2 0.17936839745830468 1108 3 0.13388613974306196 1108 4 0.1061822313498837 1108 5 0.0859940313498555 1108 6 0.17044602076961013 1108 7 0.13523392635361375 1108 8 0.09432163792558157 1108 9 0.20056859747796096 1108 10-11 0.19650704814952746 1108 12-13 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-0.49235024365239255 2316 64-65 -0.5565321913067081 2316 66-67 -0.46761493647448305 2316 68-69 -0.3649087418324619 2316 70-71 -0.40915988783638824 2316 72-73 -0.28775808764775235 2316 74-75 -0.1463507030287836 2316 76-77 -0.23900693752570845 2316 78-79 -0.15513106413703426 2316 80-81 -0.4667027439261595 2316 82-83 -0.1497146417415003 2316 84-85 -0.06440296135660972 2316 86-87 -0.0012627684806680861 2316 88-89 0.10488940658028412 2316 90-91 0.06558779843373941 2316 92-93 0.04143333586969078 2316 94-95 0.12646841404824016 2316 96-97 0.14311516911232047 2316 98-99 0.2317493984357526 2316 100-101 0.2851892943723797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 55.0 5 112.0 6 265.0 7 714.0 8 1698.0 9 3087.0 10 4946.0 11 7679.0 12 10678.0 13 14228.0 14 18756.0 15 24887.0 16 31009.0 17 38648.0 18 36488.0 19 31661.0 20 31105.0 21 32049.0 22 34510.0 23 38900.0 24 44769.0 25 52899.0 26 63326.0 27 76079.0 28 91566.0 29 108426.0 30 129726.0 31 154332.0 32 186881.0 33 230530.0 34 296066.0 35 390132.0 36 505040.0 37 522401.0 38 212987.0 39 7833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22844967144877 18.083361867559265 15.003936558516125 23.68425190247584 2 16.60310302437092 19.781139484615966 37.72234165894698 25.89341583206614 3 19.81089911290353 22.6027766203916 29.46521770652355 28.121106560181314 4 14.089873474848558 16.19859688251111 34.46576570936921 35.24576393327112 5 14.591500582271664 36.583971267603665 33.21151673899892 15.61301141112576 6 34.730287858913755 34.747058883474864 15.784445748210436 14.738207509400944 7 30.52265325761138 31.958439304759505 18.86699500217645 18.651912435452665 8 28.336975304957495 33.441102694864895 18.606170630616727 19.615751369560883 9 27.06581316903941 16.144993659912153 18.798688016724437 37.990505154324 10-11 26.028630118838432 25.545065577326508 26.112470683462924 22.313833620372137 12-13 27.215689642873258 22.661614007299473 26.45276816533435 23.669928184492917 14-15 23.385868955989164 24.212628057399996 24.630345946056114 27.771157040554723 16-17 23.353418770445145 27.385947570166792 25.779728897552623 23.480904761835436 18-19 23.736619211849195 25.379742873406425 28.01355075491448 22.870087159829904 20-21 23.51970231431404 24.667367400447517 28.625329196868826 23.187601088369615 22-23 23.64640216392803 24.52747783880891 28.636902950797715 23.189217046465345 24-25 23.734632159605855 24.970494124957 28.20412256799454 23.09075114744261 26-27 22.746950581201503 25.407004119470766 28.108814362908863 23.737230936418865 28-29 23.17471709814689 25.914348397653804 27.86459144648631 23.046343057713 30-31 23.058062393452314 25.28571158205209 27.683735163393745 23.97249086110185 32-33 22.629411310833177 25.235544090179197 27.83681443705698 24.298230161930647 34-35 23.913905989811322 25.327927366259605 27.507897614707055 23.25026902922201 36-37 24.176268760405463 24.55581520887793 27.52299174201498 23.744924288701625 38-39 23.25289743644246 25.089034859502394 27.473445322215778 24.184622381839365 40-41 23.887182487090854 24.92339390724049 27.687954087737797 23.501469517930857 42-43 23.51755535555757 25.117739837687168 27.318233711721636 24.046471095033628 44-45 23.34015626751531 25.313765196921235 27.867284709979195 23.47879382558426 46-47 24.1287874927755 25.809878890491905 26.692963594356666 23.368370022375924 48-49 23.649648638300537 25.872930372587525 26.386484767047264 24.09093622206467 50-51 23.924419747299094 26.065433200514192 26.639214789947285 23.370932262239428 52-53 24.568342652995565 25.351106931295575 26.66006210520033 23.42048831050853 54-55 23.535687197098845 25.90319683097515 27.014058835432863 23.547057136493137 56-57 23.650565803706222 25.934802642018695 26.788465261996304 23.62616629227878 58-59 23.663930213903352 25.34446840074014 26.54416442591997 24.44743695943654 60-61 22.836312179811006 25.945066159653745 27.120013277061112 24.098608383474136 62-63 23.336953467685934 26.02641727531995 27.167225458182354 23.46940379881176 64-65 22.79910146906605 26.07600243995114 26.8250062964133 24.299889794569506 66-67 22.693291265988897 26.01229642919639 27.06887159181865 24.225540712996064 68-69 23.728216422456114 25.421360746409633 27.071965730937077 23.77845710019718 70-71 23.22889485321564 25.87538368097309 26.399798631181188 24.49592283463008 72-73 23.244290645349608 26.008015734482072 26.498655551980715 24.24903806818761 74-75 23.224229471872867 26.55054090921664 26.328834370118457 23.896395248792036 76-77 23.43332862618813 25.83472970553167 26.71410207323056 24.017839595049637 78-79 23.510326845722588 26.181330879794558 26.618396591056324 23.68994568342653 80-81 23.84900538033198 26.237318397235544 26.172883109350586 23.740793113081892 82-83 23.547743198484426 26.430732396100886 26.275701355680862 23.745823049733826 84-85 23.291755099758934 26.63732857277215 26.32237621963417 23.74854010783475 86-87 23.89587557409462 26.789980694027932 25.526422947391993 23.787720784485458 88-89 23.34668756641267 26.946317962935705 25.340942028827268 24.36605244182436 90-91 23.12668552297727 26.829245567049092 25.751253350663898 24.292815559309737 92-93 23.39445046914801 26.314201854405212 26.10549428216082 24.18585339428596 94-95 22.97740102466391 26.050315446942374 26.322466321074558 24.64981720731916 96-97 23.380893461905384 25.549101912959827 26.32211232249753 24.747892302637258 98-99 22.970071565148043 26.15032262403796 25.72686856546221 25.152737245351787 100-101 23.104873672803038 25.757942800307703 26.211561295492146 24.925622231397114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 565.0 1 390.5 2 335.5 3 486.0 4 448.5 5 404.5 6 733.0 7 1711.0 8 2665.0 9 2980.5 10 2584.0 11 2069.5 12 2072.0 13 2279.5 14 2601.5 15 2918.0 16 3212.0 17 3504.5 18 3893.5 19 4434.0 20 5033.0 21 5909.5 22 6996.5 23 8294.0 24 10011.0 25 12454.0 26 15740.0 27 19473.0 28 22479.5 29 25415.5 30 29387.5 31 36744.5 32 51289.5 33 53498.5 34 45262.5 35 45412.5 36 49832.0 37 55511.0 38 61109.5 39 66054.5 40 72699.0 41 82451.5 42 89286.5 43 95199.0 44 101321.0 45 110880.0 46 117748.0 47 124657.0 48 129963.5 49 127851.5 50 129651.0 51 129946.5 52 130235.0 53 139208.5 54 149780.5 55 144876.5 56 133168.5 57 124765.5 58 120174.0 59 109090.0 60 86817.5 61 72232.0 62 63740.5 63 47983.0 64 32847.5 65 25110.5 66 18776.5 67 14203.0 68 10899.0 69 8278.0 70 6381.0 71 4640.5 72 3432.0 73 2901.0 74 2247.5 75 1654.0 76 1232.0 77 819.0 78 539.0 79 358.5 80 233.5 81 144.0 82 74.0 83 36.5 84 23.0 85 11.0 86 6.5 87 5.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.696763279608793E-4 2 0.04993456097621339 3 9.317235867281799E-4 4 0.0 5 0.0904645369988892 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.7660543980992836E-4 26-27 0.009200770418940775 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011311706670121808 36-37 2.9116362085255622E-5 38-39 0.004833316106152433 40-41 0.00598341240852003 42-43 4.6586179336408995E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.8232724170511244E-5 68-69 7.861417763019017E-4 70-71 2.9116362085255622E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012083290265381083 82-83 6.260017848329958E-4 84-85 0.004833316106152433 86-87 0.0019071217165842432 88-89 1.0190726729839467E-4 90-91 0.0029407525706108177 92-93 7.133508710887627E-4 94-95 8.734908625576686E-5 96-97 1.4558181042627811E-5 98-99 5.8232724170511244E-5 100-101 2.9116362085255622E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3434495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.95838715662037 #Duplication Level Percentage of deduplicated Percentage of total 1 67.7582806007208 25.719950481090798 2 15.32356876666365 11.633159117322291 3 6.140191434726261 6.992152910853113 4 3.159697695082439 4.797481136312809 5 1.8406777444479545 3.49345792271651 6 1.2019344254943312 2.7374095355870343 7 0.8258613390193184 2.1943855110926136 8 0.5920226962439011 1.797778136762581 9 0.4557702677581523 1.557027385023643 >10 2.4073536599175953 16.61925894968125 >50 0.17696306791991925 4.656554239134902 >100 0.10059618533891665 7.177456282199858 >500 0.009772201877641546 2.5819845434812465 >1k 0.006309610660250645 5.361003822765437 >5k 9.233576575976553E-4 2.372770221161868 >10k+ 7.694647146647128E-5 0.3081698048139894 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10551 0.30720673636153206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8762 0.2551175645910097 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 8234 0.2397441254099948 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 7937 0.23109656587067384 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 7332 0.21348116680909418 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7297 0.21246209413611025 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6957 0.20256253102712332 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 6545 0.190566589847998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6197 0.18043409584232908 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 5952 0.17330058713144145 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5638 0.16415804943667117 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5365 0.15620928258739641 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 5022 0.14622237039215372 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4882 0.14214607970021795 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4880 0.14208784697604743 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4640 0.13509992007558608 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 4555 0.13262502929833936 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4453 0.12965516036564328 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4373 0.12732585139882283 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4342 0.1264232441741799 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 4136 0.12042527358461724 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 4039 0.11760098646234746 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3828 0.11145743406235853 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3782 0.11011808140643675 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3776 0.10994338323392522 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3442 0.10021851829744985 No Hit GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG 3439 0.10013116921119408 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.911636208525562E-5 2 0.0 0.0 0.0 0.0 2.911636208525562E-5 3 0.0 0.0 0.0 0.0 2.911636208525562E-5 4 0.0 0.0 0.0 0.0 2.911636208525562E-5 5 0.0 0.0 0.0 0.0 2.911636208525562E-5 6 0.0 0.0 0.0 0.0 2.911636208525562E-5 7 0.0 0.0 0.0 0.0 2.911636208525562E-5 8 0.0 0.0 0.0 0.0 2.911636208525562E-5 9 0.0 0.0 0.0 0.0 2.911636208525562E-5 10-11 0.0 0.0 0.0 0.0 2.911636208525562E-5 12-13 2.911636208525562E-5 0.0 0.0 0.0 3.05721801895184E-4 14-15 2.911636208525562E-5 0.0 0.0 0.0 3.9307088815095087E-4 16-17 2.911636208525562E-5 0.0 0.0 0.0 5.095363364919733E-4 18-19 2.911636208525562E-5 0.0 0.0 0.0 6.696763279608793E-4 20-21 2.911636208525562E-5 0.0 0.0 4.367454312788343E-5 7.570254142166462E-4 22-23 2.911636208525562E-5 0.0 0.0 7.279090521313905E-5 8.298163194297852E-4 24-25 5.823272417051124E-5 0.0 0.0 2.4748907772467277E-4 8.589326815150408E-4 26-27 5.823272417051124E-5 0.0 0.0 7.279090521313905E-4 9.753981298560633E-4 28-29 1.0190726729839467E-4 0.0 0.0 0.002984427113738701 0.0010190726729839468 30-31 1.1646544834102247E-4 0.0 0.0 0.011268032126993925 0.0010336308540265745 32-33 1.1646544834102247E-4 0.0 0.0 0.030441156560134748 0.001077305397154458 34-35 1.3102362938365028E-4 0.0 0.0 0.05673323152312058 0.0011064217592397136 36-37 1.455818104262781E-4 0.0 0.0 0.09966530741782999 0.0011064217592397136 38-39 1.455818104262781E-4 0.0 0.0 0.16670573111913106 0.0011792126644528528 40-41 1.455818104262781E-4 0.0 0.0 0.23997705630667682 0.0011937708454954805 42-43 1.7469817251153372E-4 0.0 0.0 0.31924635208378527 0.0012520035696659917 44-45 2.0381453459678933E-4 0.0 0.0 0.4171064450523294 0.0012956781127938751 46-47 2.4748907772467277E-4 1.455818104262781E-5 0.0 0.5313008171507019 0.0013393526559217586 48-49 2.6204725876730056E-4 2.911636208525562E-5 0.0 0.651420368933424 0.001455818104262781 50-51 2.6204725876730056E-4 2.911636208525562E-5 0.0 0.7911643487616082 0.001455818104262781 52-53 2.6204725876730056E-4 2.911636208525562E-5 0.0 0.9206739273168254 0.0016450744578169426 54-55 2.6204725876730056E-4 2.911636208525562E-5 0.0 1.0596026490066226 0.0016596326388595704 56-57 2.911636208525562E-4 2.911636208525562E-5 0.0 1.2247797711162776 0.001746981725115337 58-59 2.911636208525562E-4 2.911636208525562E-5 0.0 1.4020110671292287 0.0017760980872005927 60-61 2.911636208525562E-4 2.911636208525562E-5 0.0 1.5986629766530451 0.0017906562682432204 62-63 2.911636208525562E-4 2.911636208525562E-5 0.0 1.7915152009247355 0.0018925635355416153 64-65 2.911636208525562E-4 2.911636208525562E-5 0.0 1.996072202754699 0.001921679897626871 66-67 2.911636208525562E-4 2.911636208525562E-5 0.0 2.2366461444841237 0.0019362380786694987 68-69 2.911636208525562E-4 2.911636208525562E-5 0.0 2.5003530358902837 0.0019507962597121265 70-71 2.911636208525562E-4 2.911636208525562E-5 0.0 2.775153261250926 0.0020381453459678935 72-73 2.911636208525562E-4 2.911636208525562E-5 0.0 3.074076392599203 0.002154610794308916 74-75 2.911636208525562E-4 2.911636208525562E-5 0.0 3.370815796791086 0.0023147507857778217 76-77 2.911636208525562E-4 2.911636208525562E-5 0.0 3.691139454272025 0.002358425328905705 78-79 2.911636208525562E-4 2.911636208525562E-5 0.0 4.063275678083677 0.002431216234118844 80-81 3.202799829378118E-4 2.911636208525562E-5 0.0 4.4443360668744605 0.002445774415161472 82-83 3.202799829378118E-4 2.911636208525562E-5 0.0 4.849577594377048 0.002533123501417239 84-85 3.202799829378118E-4 2.911636208525562E-5 0.0 5.2676303211971485 0.0025622398635024943 86-87 3.202799829378118E-4 2.911636208525562E-5 0.0 5.698814527317699 0.0026350307687156337 88-89 3.202799829378118E-4 2.911636208525562E-5 0.0 6.1512536777604865 0.0027078216739287726 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6190 0.0 53.174976 1 GTATCAA 10830 0.0 41.240982 1 TATCAAC 14320 0.0 31.122593 2 ATCAACG 14545 0.0 30.429857 3 TCAACGC 14585 0.0 30.345963 4 AAGGTAA 2380 0.0 29.778385 1 AGGTAAG 2480 0.0 29.727556 2 CAACGCA 15050 0.0 29.313597 5 TAAGGTA 1170 0.0 28.442635 4 AACGCAG 15810 0.0 28.088758 6 TAAGGTG 2950 0.0 27.556988 5 GTGTAAG 1335 0.0 27.078476 1 ACGCAGA 17865 0.0 24.804554 7 CGCAGAG 18140 0.0 24.271423 8 GTAAGGT 3200 0.0 24.215603 4 GGTAAGG 3400 0.0 22.511837 3 GTACATG 12470 0.0 22.161621 1 TACATGG 12230 0.0 22.090273 2 GCAGAGT 19980 0.0 21.798502 9 GGACCGA 1665 0.0 21.394386 6 >>END_MODULE