FastQCFastQC Report
Thu 26 May 2016
SRR938621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445551
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105290.30558247432703795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.2517739542964246No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA83260.24164495025614188No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA80270.23296709292650147No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC75140.2180783276753123No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA71530.20760104842447552No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA70160.20362490643731582No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG66460.19288642077856344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62620.1817416140408312No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA58800.17065485317152468No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA56220.16316693614461084No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA52710.15297988623590247No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA50160.14557903801162717No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT49790.1445051894457519No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG49430.14346036381408953No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG46760.13571124037926008No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA44130.12807820868128206No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG44070.12790407107600496No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT43340.12578539687846732No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT42680.12386988322041961No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT40160.11655610379878284No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG39710.11525007175920483No Hit
TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA38660.11220266366685618No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT37540.10895209503501763No Hit
GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG37220.10802336114020661No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA35800.10390210448198271No Hit
GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG35030.1016673385475937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.054.411311
GTATCAA108350.042.7191281
TATCAAC144450.031.7466552
ATCAACG144150.031.7310433
TCAACGC147250.031.062574
AGGTAAG26800.030.8853782
AAGGTAA26250.030.4451711
TAAGGTG29700.030.2567985
CAACGCA151900.030.1116735
GTGTAAG13600.029.7316131
AACGCAG158100.028.8684566
TGGTATC28250.026.942632
GTAAGGT32900.026.5912954
TAAGGTA13500.026.4146654
GGTAAGG34450.025.8093033
GTGGTAT30100.025.2866881
ACGCAGA180550.025.0684457
CGCAGAG183250.024.6472578
TGTAAGG16250.023.1266212
GTACATG124900.022.5474661
Produced by FastQC (version 0.11.5)