##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938621_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3445551 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80337223277206 31.0 31.0 34.0 30.0 34.0 2 32.03869279543388 31.0 31.0 34.0 30.0 34.0 3 32.100630929566854 33.0 31.0 34.0 30.0 34.0 4 35.78003314999546 37.0 35.0 37.0 35.0 37.0 5 35.64591555893382 37.0 35.0 37.0 33.0 37.0 6 35.68983654573681 37.0 35.0 37.0 33.0 37.0 7 35.621187438525794 37.0 35.0 37.0 33.0 37.0 8 35.59557992321112 37.0 35.0 37.0 33.0 37.0 9 37.164866519172115 39.0 37.0 39.0 33.0 39.0 10-11 37.09743390824863 39.0 37.0 39.0 33.0 39.0 12-13 36.99044797189187 39.0 37.0 39.0 33.0 39.0 14-15 38.041147700324274 40.0 37.0 41.0 33.0 41.0 16-17 37.9657295741668 40.0 37.0 41.0 33.0 41.0 18-19 37.96333910019036 40.0 37.0 41.0 33.0 41.0 20-21 37.93451903628767 40.0 37.0 41.0 32.5 41.0 22-23 37.96748328496661 40.0 37.0 41.0 33.0 41.0 24-25 37.88609107803077 40.0 37.0 41.0 32.5 41.0 26-27 37.70150956987722 40.0 37.0 41.0 32.0 41.0 28-29 37.56533265651851 39.5 36.5 41.0 32.0 41.0 30-31 37.294887523069605 39.0 36.0 41.0 31.0 41.0 32-33 37.05267357818822 39.0 36.0 41.0 31.0 41.0 34-35 36.875206897242265 39.0 35.0 40.5 30.0 41.0 36-37 36.86658795646908 39.0 35.0 41.0 30.5 41.0 38-39 36.69404356516563 39.0 35.0 41.0 30.0 41.0 40-41 36.49047670459674 39.0 35.0 40.5 30.0 41.0 42-43 36.22788198462307 39.0 35.0 40.0 30.0 41.0 44-45 35.928802388935765 38.0 35.0 40.0 29.0 41.0 46-47 35.54316290776134 38.0 34.5 40.0 27.5 41.0 48-49 35.10347532223439 38.0 34.0 40.0 26.5 41.0 50-51 34.68778926795743 38.0 33.5 40.0 26.0 41.0 52-53 34.1261252554381 37.0 33.0 40.0 24.0 41.0 54-55 33.76403745003339 37.0 33.0 40.0 23.0 41.0 56-57 33.36343780718962 36.5 32.0 39.5 22.5 41.0 58-59 32.87129155830229 36.0 31.5 39.0 21.5 40.0 60-61 32.42055160408306 35.5 31.0 39.0 20.0 40.0 62-63 31.91372468438285 35.0 31.0 38.5 18.0 40.0 64-65 31.37983344318514 35.0 30.0 38.0 16.0 40.0 66-67 30.866198758921286 34.5 29.5 37.0 12.0 39.5 68-69 30.29463052498715 34.0 29.0 36.5 8.0 39.0 70-71 29.70444103134738 34.0 28.5 36.0 7.0 39.0 72-73 29.13644479504149 33.5 27.5 35.5 4.0 38.5 74-75 28.53736717291371 33.0 26.0 35.0 2.0 37.5 76-77 27.318532217343467 31.5 25.5 34.0 2.0 36.0 78-79 27.943807391038472 33.0 26.0 35.0 2.0 36.0 80-81 27.690198171497098 33.0 26.0 35.0 2.0 36.0 82-83 27.220281168382066 33.0 25.5 35.0 2.0 36.0 84-85 26.657328827813025 32.0 25.0 35.0 2.0 35.0 86-87 26.055321050247116 32.0 23.5 34.5 2.0 35.0 88-89 25.305645163864938 31.0 20.0 34.0 2.0 35.0 90-91 24.785358568194173 31.0 18.5 34.0 2.0 35.0 92-93 24.039435202091045 31.0 13.5 34.0 2.0 35.0 94-95 23.266784470756637 30.5 4.5 34.0 2.0 35.0 96-97 22.29149822481223 30.0 2.0 34.0 2.0 35.0 98-99 21.107558558848787 29.0 2.0 34.0 2.0 35.0 100-101 17.98700106311008 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2986956924625197 1101 2 0.34801242980437763 1101 3 0.3114909152504879 1101 4 0.22922864914193042 1101 5 0.17876652980616115 1101 6 0.2656944637650227 1101 7 0.30563330507807507 1101 8 0.244797950208671 1101 9 0.37989817366756995 1101 10-11 0.3062751167201654 1101 12-13 0.31105311433136507 1101 14-15 0.529061357522977 1101 16-17 0.478619988633298 1101 18-19 0.5800101904672275 1101 20-21 0.5437907355333564 1101 22-23 0.46443339161703534 1101 24-25 0.46834838444348037 1101 26-27 0.49839079809274267 1101 28-29 0.41563677089660445 1101 30-31 0.42243990814828436 1101 32-33 0.4498610162828456 1101 34-35 0.2261979655698667 1101 36-37 0.46641005340791253 1101 38-39 0.6789383189762077 1101 40-41 0.6905123744695061 1101 42-43 0.595558512654101 1101 44-45 0.6423775217044678 1101 46-47 0.7616693158056194 1101 48-49 0.8353905649998481 1101 50-51 0.7942361427366151 1101 52-53 0.731745754236151 1101 54-55 0.7787966333267065 1101 56-57 0.8141118126111593 1101 58-59 0.8859085108136284 1101 60-61 0.8137730960832812 1101 62-63 0.9843249285830176 1101 64-65 1.0212322791924926 1101 66-67 1.2413058607193541 1101 68-69 1.2194199205869651 1101 70-71 1.272373509709091 1101 72-73 1.2164686833208194 1101 74-75 1.1932127856924346 1101 76-77 1.1313022983740986 1101 78-79 1.495527572097064 1101 80-81 1.8956911831966003 1101 82-83 1.9464724668067745 1101 84-85 2.136547386846985 1101 86-87 2.0689986364734416 1101 88-89 2.3210416851147393 1101 90-91 2.3831890907653275 1101 92-93 2.6232648517369874 1101 94-95 2.661755121102072 1101 96-97 3.1408897571123937 1101 98-99 3.4436903021004888 1101 100-101 3.9747843495400375 1102 1 0.35931626553686513 1102 2 0.3008188794769069 1102 3 0.31882381508558666 1102 4 0.19177351772868434 1102 5 0.1432861827238625 1102 6 0.15805526878210685 1102 7 0.22665703887589927 1102 8 0.25736728021809085 1102 9 0.3566827129798966 1102 10-11 0.30863618979501695 1102 12-13 0.3788823226609779 1102 14-15 0.47550590286522265 1102 16-17 0.43511430520988625 1102 18-19 0.5244681606214385 1102 20-21 0.5575870516090191 1102 22-23 0.429664364835304 1102 24-25 0.4424236058744029 1102 26-27 0.46019049372281984 1102 28-29 0.4631894357270312 1102 30-31 0.43161646508720963 1102 32-33 0.22841683710197458 1102 34-35 0.37018330658678167 1102 36-37 0.5262535813650047 1102 38-39 0.5402855312537298 1102 40-41 0.5776497837363195 1102 42-43 0.6279568862896028 1102 44-45 0.5830176952363999 1102 46-47 0.661405117702266 1102 48-49 0.6598697937009277 1102 50-51 0.785656497938021 1102 52-53 0.8995570552015479 1102 54-55 0.7048215789114423 1102 56-57 0.9100677420719876 1102 58-59 1.0236762933835948 1102 60-61 0.9591171030900014 1102 62-63 0.9607241463701861 1102 64-65 1.0553094682226778 1102 66-67 1.1915262438135166 1102 68-69 1.2158082797316858 1102 70-71 1.3560698494493728 1102 72-73 1.3711287109980148 1102 74-75 1.3805424227762977 1102 76-77 1.2927536294467181 1102 78-79 1.8027060209158563 1102 80-81 2.0829356129147563 1102 82-83 1.929606290547646 1102 84-85 2.1303079968911938 1102 86-87 2.3001398283946024 1102 88-89 2.307336563949402 1102 90-91 2.527388463473308 1102 92-93 2.877698144916927 1102 94-95 3.038207618824732 1102 96-97 3.387310748375274 1102 98-99 3.732723543013769 1102 100-101 3.8993114417516352 1103 1 0.25938849656988694 1103 2 0.22380210584571358 1103 3 0.22766612932829844 1103 4 0.18942665558795824 1103 5 0.13242567046933118 1103 6 0.12714866098448852 1103 7 0.22314026760753336 1103 8 0.1938100342489122 1103 9 0.22607917949946454 1103 10-11 0.3162487575489479 1103 12-13 0.3881783344576135 1103 14-15 0.49302823666319284 1103 16-17 0.4413786822036343 1103 18-19 0.37855777364553234 1103 20-21 0.487237984286331 1103 22-23 0.4170176034756423 1103 24-25 0.3901056370385092 1103 26-27 0.4333242229581771 1103 28-29 0.3247418670667557 1103 30-31 0.47015552401163774 1103 32-33 0.6343970601898548 1103 34-35 0.5269999910726995 1103 36-37 0.6147070838300479 1103 38-39 0.7269272750487232 1103 40-41 0.6727303787146042 1103 42-43 0.6636894472564379 1103 44-45 0.8841067958401183 1103 46-47 0.8294927768060347 1103 48-49 0.8355855349392485 1103 50-51 0.9111819249825004 1103 52-53 1.121482334556255 1103 54-55 1.1090464924923253 1103 56-57 1.1317537522289172 1103 58-59 1.1902488038092116 1103 60-61 1.2597940026498051 1103 62-63 1.3272210732299605 1103 64-65 1.1849125470604385 1103 66-67 1.408315772783542 1103 68-69 1.4276397603893969 1103 70-71 1.470859325099866 1103 72-73 1.5091861464833798 1103 74-75 1.6823743412248682 1103 76-77 1.542320229874111 1103 78-79 1.6809573647599123 1103 80-81 2.0323278370536855 1103 82-83 2.2044477340234536 1103 84-85 2.246323108611225 1103 86-87 2.3466716019798746 1103 88-89 2.4702331469605703 1103 90-91 2.5859560528087187 1103 92-93 2.700163699202502 1103 94-95 2.6860978280111887 1103 96-97 3.179835821709702 1103 98-99 3.576426055300221 1103 100-101 4.012092830486644 1104 1 0.27322353018749723 1104 2 0.3021337063479095 1104 3 0.24009980383340235 1104 4 0.2245987967746288 1104 5 0.09698629335887432 1104 6 0.15094449342482363 1104 7 0.17300248587667255 1104 8 0.2430331590070054 1104 9 0.18265471408494705 1104 10-11 0.2683398143306661 1104 12-13 0.2980965007457712 1104 14-15 0.3431187461086864 1104 16-17 0.4681670252628649 1104 18-19 0.476725337945318 1104 20-21 0.44417479336761545 1104 22-23 0.479750207433284 1104 24-25 0.3992986471553124 1104 26-27 0.3879595955777688 1104 28-29 0.4135741412762002 1104 30-31 0.47127716875782966 1104 32-33 0.48451805820111105 1104 34-35 0.3352341164998265 1104 36-37 0.46278912148509477 1104 38-39 0.6865227213691583 1104 40-41 0.6942858804684846 1104 42-43 0.7056118683933263 1104 44-45 0.6679406347862269 1104 46-47 0.6891133429602618 1104 48-49 0.8273950454678314 1104 50-51 0.7238113781033917 1104 52-53 0.875315124627086 1104 54-55 0.9126809610894853 1104 56-57 0.8743522133582289 1104 58-59 0.985003424027596 1104 60-61 1.0255532678505048 1104 62-63 1.1238570223450104 1104 64-65 1.012413816559718 1104 66-67 1.139417300222096 1104 68-69 1.252959060577755 1104 70-71 1.2146930662767303 1104 72-73 1.3200542095520866 1104 74-75 1.453347022526561 1104 76-77 1.3347809607300611 1104 78-79 1.6494078312178182 1104 80-81 1.8558070172225847 1104 82-83 2.09392432467968 1104 84-85 2.1348214922884274 1104 86-87 2.3875643586427806 1104 88-89 2.554717477252847 1104 90-91 2.672675053463003 1104 92-93 2.9060787553996583 1104 94-95 3.0560056479123574 1104 96-97 3.092166572648651 1104 98-99 3.48557861982599 1104 100-101 3.91460171783924 1105 1 0.24916229353271646 1105 2 0.24341388827247812 1105 3 0.2009348512052327 1105 4 0.1535167440586207 1105 5 0.06659318241718637 1105 6 0.13672457025655405 1105 7 0.1678474226767861 1105 8 0.1500880377287217 1105 9 0.2322221016321322 1105 10-11 0.21108545526507072 1105 12-13 0.3483635133488221 1105 14-15 0.4501935651043425 1105 16-17 0.44776467775101736 1105 18-19 0.40165931488958506 1105 20-21 0.3600944758066973 1105 22-23 0.2400245336484872 1105 24-25 0.22940465409543975 1105 26-27 0.36226433475432884 1105 28-29 0.45924958109157643 1105 30-31 0.5494728590609554 1105 32-33 0.6136264306083135 1105 34-35 0.5703925608055656 1105 36-37 0.6790382064666929 1105 38-39 0.672357734023187 1105 40-41 0.5981750671136155 1105 42-43 0.6133941464156365 1105 44-45 0.6577193451251091 1105 46-47 0.8969985817415704 1105 48-49 0.926996999696371 1105 50-51 0.8809787629385042 1105 52-53 1.0137964060523146 1105 54-55 1.0425188975545936 1105 56-57 0.9813634375023454 1105 58-59 1.0741853393672898 1105 60-61 1.0984720558772239 1105 62-63 1.2205732733905315 1105 64-65 1.3423013872339347 1105 66-67 1.4242637049727342 1105 68-69 1.5386901975564946 1105 70-71 1.5944000349245897 1105 72-73 1.619503209381115 1105 74-75 1.8416487312062664 1105 76-77 1.6511102479277575 1105 78-79 1.8715806724666884 1105 80-81 2.055401684796326 1105 82-83 2.147528902920829 1105 84-85 2.200182336501303 1105 86-87 2.354332883267549 1105 88-89 2.2889177509598646 1105 90-91 2.6159759342251476 1105 92-93 2.8072456576164306 1105 94-95 2.8049475104548414 1105 96-97 3.2760266812933274 1105 98-99 3.7304774509142256 1105 100-101 4.2529994804767846 1106 1 0.14919812480738415 1106 2 0.1585969730036325 1106 3 0.12585779216067294 1106 4 0.03087712019252109 1106 5 0.0382210633643254 1106 6 0.10471772088672537 1106 7 0.1079634055330132 1106 8 0.04664612763833276 1106 9 0.11837531267398305 1106 10-11 0.25313232954962217 1106 12-13 0.12401757947170466 1106 14-15 0.12113309310542775 1106 16-17 0.14862477370325422 1106 18-19 0.25145759135440215 1106 20-21 0.4126210894314468 1106 22-23 0.21665098643224212 1106 24-25 0.10294496573304457 1106 26-27 0.11028943554341453 1106 28-29 0.1728014343243487 1106 30-31 0.26112340165401804 1106 32-33 0.3324196071125911 1106 34-35 0.28645187646456094 1106 36-37 0.34454816981412506 1106 38-39 0.19321982140934324 1106 40-41 0.17262380695022017 1106 42-43 0.3764992715961526 1106 44-45 -0.06428665388789767 1106 46-47 -0.1357426997388913 1106 48-49 0.3047687450283121 1106 50-51 0.22705872700548468 1106 52-53 0.5171705783171703 1106 54-55 0.45610265733824207 1106 56-57 0.4875452408838541 1106 58-59 0.7527068523047049 1106 60-61 0.5930548468355283 1106 62-63 0.7835836237593448 1106 64-65 0.45810036288102296 1106 66-67 0.6695367748989547 1106 68-69 0.5644912591215991 1106 70-71 0.7393662495938997 1106 72-73 1.104018356997969 1106 74-75 1.3173743145183394 1106 76-77 1.3956517929461576 1106 78-79 1.6435411374156352 1106 80-81 1.6096166564547332 1106 82-83 1.8993707976030478 1106 84-85 1.8903697275266218 1106 86-87 2.2375410978598183 1106 88-89 2.5389800741201647 1106 90-91 2.3751894050401035 1106 92-93 2.218204213259927 1106 94-95 2.7753801676454835 1106 96-97 3.2390739531378685 1106 98-99 3.6656184829566882 1106 100-101 3.787309197874869 1107 1 0.24244824074547822 1107 2 0.20251528409115593 1107 3 0.21135242968670553 1107 4 0.20624291650444349 1107 5 0.21285326926568615 1107 6 0.14480387827337893 1107 7 0.20279478334200718 1107 8 0.17704772655579148 1107 9 0.2575513785166521 1107 10-11 0.257182206816708 1107 12-13 0.2739077637552114 1107 14-15 0.3371717158351686 1107 16-17 0.346063650226192 1107 18-19 0.28806412034033 1107 20-21 0.262200064677117 1107 22-23 0.2936210563031736 1107 24-25 0.2977754208851522 1107 26-27 0.2975157123734675 1107 28-29 0.2980972220907461 1107 30-31 0.30002285027270403 1107 32-33 0.3694936807435809 1107 34-35 0.4119448234955314 1107 36-37 0.3355743542690348 1107 38-39 0.45595700189346644 1107 40-41 0.5065698079090026 1107 42-43 0.552978042316937 1107 44-45 0.5341237334145319 1107 46-47 0.5861183179532077 1107 48-49 0.7008879407248543 1107 50-51 0.625445938418153 1107 52-53 0.7690333091797967 1107 54-55 0.7261200306545206 1107 56-57 0.715109450960945 1107 58-59 0.7559047575921056 1107 60-61 0.835750512451547 1107 62-63 1.0473099627905533 1107 64-65 1.1078075405181984 1107 66-67 1.1842986985384591 1107 68-69 1.1639736486121315 1107 70-71 1.1388452455837026 1107 72-73 1.3988827627956013 1107 74-75 1.4052511660971767 1107 76-77 1.501444569058851 1107 78-79 1.7460712267812077 1107 80-81 1.9483392442601932 1107 82-83 1.9087523805741817 1107 84-85 2.2054584984561743 1107 86-87 2.404451603973868 1107 88-89 2.5655677613579364 1107 90-91 2.675799382886172 1107 92-93 2.922806347584224 1107 94-95 2.8876631702806073 1107 96-97 3.3481066623407365 1107 98-99 3.5499733342904705 1107 100-101 3.936138717703116 1108 1 0.13125608867681038 1108 2 0.12791673682844618 1108 3 0.09380745820520531 1108 4 0.04267069776135912 1108 5 0.005066387400901817 1108 6 0.03015170647988441 1108 7 0.10374465003067002 1108 8 0.1157650877788825 1108 9 0.12475079052179439 1108 10-11 0.10596909243145802 1108 12-13 0.1724896011641306 1108 14-15 0.2936532341218907 1108 16-17 0.292765704083223 1108 18-19 0.24773431072616603 1108 20-21 0.24524507553326913 1108 22-23 0.19438814959110573 1108 24-25 0.15642213272642636 1108 26-27 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0.7475283321222115 2316 76-77 0.7586384475483179 2316 78-79 0.888178056687746 2316 80-81 0.9104907711399868 2316 82-83 1.225153856333126 2316 84-85 1.3581890750169627 2316 86-87 1.350129597213833 2316 88-89 1.5536120636790365 2316 90-91 1.6828540210456353 2316 92-93 1.5172125611700018 2316 94-95 2.1411258235113486 2316 96-97 2.3921313108613553 2316 98-99 2.6816334261148214 2316 100-101 3.153661973743475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 18.0 4 62.0 5 131.0 6 280.0 7 753.0 8 1782.0 9 3133.0 10 5001.0 11 7648.0 12 10937.0 13 14644.0 14 19237.0 15 25732.0 16 32464.0 17 40419.0 18 38889.0 19 34755.0 20 34383.0 21 35770.0 22 39237.0 23 44385.0 24 50647.0 25 58528.0 26 69199.0 27 82374.0 28 97981.0 29 116440.0 30 137333.0 31 162615.0 32 195065.0 33 237367.0 34 296051.0 35 377256.0 36 470088.0 37 480658.0 38 214514.0 39 9766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19111805339698 18.07121705643016 15.002854405579832 23.73481048459303 2 16.61287723378586 19.758651678215788 37.7575686305254 25.870902457472955 3 19.805087382427857 22.595880360256608 29.450414796618475 28.14861746069706 4 14.136752002800131 16.18934097913512 34.392148019286324 35.28175899877842 5 14.596040150491712 36.578812045147515 33.20219055504714 15.622957249313634 6 34.71009426358803 34.74451546356446 15.790217587840086 14.75517268500742 7 30.497183179119975 31.968442783171692 18.861424486243276 18.672949551465063 8 28.324090979933253 33.3951521832067 18.61844448101334 19.662312355846716 9 27.098713674532753 16.141975550499758 18.7478287217342 38.01148205323329 10-11 25.99891860547123 25.551660677784195 26.121584036921814 22.327836679822763 12-13 27.22904116061553 22.620010558543466 26.435263909894235 23.715684370946764 14-15 23.41962141904154 24.167919731851306 24.6399342224219 27.772524626685254 16-17 23.352505883674336 27.36820613016612 25.76143844627463 23.517849539884914 18-19 23.73643576890895 25.36962883440123 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23.369091531254053 25.30655585021087 27.85660944252098 23.467743176014096 46-47 24.132003346926716 25.793030370486676 26.64878659395063 23.426179688635976 48-49 23.617441738636288 25.896351555963037 26.403643423069344 24.082563282331332 50-51 23.962408334690156 26.0650618725423 26.60739893271062 23.365130860056926 52-53 24.568116971712218 25.369048375716975 26.627831078396458 23.43500357417435 54-55 23.524582860622292 25.921514439925573 26.99637880849826 23.55752389095387 56-57 23.69120352593823 25.928567012939297 26.725072419476593 23.65515704164588 58-59 23.624276639643412 25.379424074698065 26.543272759567337 24.453026526091183 60-61 22.85783899295062 25.953526736362342 27.11158534585615 24.07704892483089 62-63 23.373315327504947 26.061027684686717 27.103342832539703 23.462314155268636 64-65 22.82148776784903 26.047285325336933 26.841918752617506 24.28930815419653 66-67 22.694473423117962 26.013864584129955 27.0404956666463 24.251166326105782 68-69 23.71842406262235 25.386013177023187 27.07431020306523 23.82125255728923 70-71 23.257455774127273 25.878763657830056 26.40236641396398 24.461414154078696 72-73 23.25385693028488 26.006275919294186 26.467798619146837 24.272068531274098 74-75 23.283445811714877 26.568348574727235 26.22502177445639 23.923183839101497 76-77 23.464403806531962 25.857141571841485 26.642893981252925 24.035560640373628 78-79 23.49615489656081 26.220334570581016 26.57982714520842 23.703683387649754 80-81 23.84740495787176 26.25947199736704 26.16294171817512 23.73018132658608 82-83 23.574792157340774 26.393064607177735 26.270789707779535 23.761353527701953 84-85 23.336621287863295 26.67164334405438 26.270006903398425 23.7217284646839 86-87 23.933260774923816 26.775968654767084 25.522884922362504 23.767885647946596 88-89 23.4104313404872 26.93666133660248 25.298461101777814 24.35444622113251 90-91 23.183904885115226 26.83891461877643 25.739631274547293 24.237549221561057 92-93 23.453850380041693 26.312282273069876 26.082333031397376 24.151534315491055 94-95 23.01341643179857 26.076888718234038 26.31738726258877 24.592307587378624 96-97 23.413932923935825 25.552807083685597 26.28906087879703 24.744199113581544 98-99 23.047402287761813 26.16915262609667 25.69996787161183 25.083477214529694 100-101 23.137174035477038 25.730843010984252 26.17711759124046 24.95486536229825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 543.0 1 393.0 2 351.0 3 485.5 4 438.5 5 427.5 6 772.5 7 1740.5 8 2729.5 9 3006.0 10 2604.5 11 2154.5 12 2141.5 13 2354.5 14 2630.0 15 2931.0 16 3216.5 17 3522.0 18 3904.5 19 4400.0 20 5033.0 21 5900.5 22 7020.5 23 8482.5 24 10310.5 25 12697.0 26 15908.5 27 19780.5 28 22726.0 29 25365.5 30 29066.5 31 36725.5 32 50989.5 33 52834.0 34 45390.5 35 45832.0 36 49651.0 37 55272.5 38 61506.5 39 66251.0 40 72660.0 41 82584.0 42 89559.0 43 95363.5 44 101913.5 45 111803.0 46 118012.5 47 124767.0 48 130027.5 49 128204.0 50 129763.5 51 129749.0 52 130185.5 53 138530.0 54 149402.0 55 145086.0 56 133331.5 57 125119.0 58 119885.5 59 109116.5 60 87745.5 61 72997.5 62 64360.5 63 48563.5 64 33707.0 65 25757.0 66 19381.5 67 14855.0 68 11324.5 69 8648.0 70 6703.0 71 4862.5 72 3605.0 73 3007.0 74 2356.5 75 1734.5 76 1229.0 77 878.5 78 590.0 79 390.0 80 258.5 81 140.0 82 73.0 83 39.0 84 24.5 85 17.0 86 7.5 87 5.5 88 5.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.045449914977314224 3 2.0316053948991032E-4 4 0.0 5 0.101609292679168 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010738485658752404 26-27 0.012044517698330398 28-29 0.0 30-31 0.0 32-33 5.224128158311979E-4 34-35 0.01554178127097814 36-37 0.003511775039754164 38-39 0.01266851078390655 40-41 0.007081595947934017 42-43 0.005906167112313821 44-45 0.002728155816007367 46-47 5.804586842568866E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.757178750220211E-4 68-69 2.3218347370275463E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010448256316623959 84-85 0.004251859862181694 86-87 0.0014801696448550608 88-89 4.3534401319266495E-5 90-91 9.72268296130285E-4 92-93 2.3218347370275463E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.691077566403748E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3445551.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.69367157367057 #Duplication Level Percentage of deduplicated Percentage of total 1 67.53217568864949 25.455356510633738 2 15.193285957089595 11.453814619827925 3 6.239885253907041 7.05612556254486 4 3.290016813708159 4.960512529910778 5 1.890861736167147 3.563676063715248 6 1.2396135483845172 2.803535158264703 7 0.8292747455435064 2.1880886931999313 8 0.6040839026248848 1.8216112182786879 9 0.44025597586700843 1.4935377746409169 >10 2.4434326212195816 16.757721337238543 >50 0.17819381369105283 4.665981058901127 >100 0.10187111452974434 7.208523952226474 >500 0.009951578696331051 2.664891239685334 >1k 0.006094377650283657 5.233242144701264 >5k 9.257282506759986E-4 2.3672155165629856 >10k+ 7.714402088966655E-5 0.306166619667498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10529 0.30558247432703795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8675 0.2517739542964246 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 8326 0.24164495025614188 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 8027 0.23296709292650147 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 7514 0.2180783276753123 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7153 0.20760104842447552 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7016 0.20362490643731582 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 6646 0.19288642077856344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6262 0.1817416140408312 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 5880 0.17065485317152468 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5622 0.16316693614461084 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5271 0.15297988623590247 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 5016 0.14557903801162717 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4979 0.1445051894457519 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4943 0.14346036381408953 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4676 0.13571124037926008 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4413 0.12807820868128206 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4407 0.12790407107600496 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 4334 0.12578539687846732 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4268 0.12386988322041961 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 4016 0.11655610379878284 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3971 0.11525007175920483 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3866 0.11220266366685618 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3754 0.10895209503501763 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3722 0.10802336114020661 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3580 0.10390210448198271 No Hit GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG 3503 0.1016673385475937 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.8045868425688664E-5 2 0.0 0.0 0.0 0.0 5.8045868425688664E-5 3 0.0 0.0 0.0 0.0 5.8045868425688664E-5 4 0.0 0.0 0.0 0.0 5.8045868425688664E-5 5 0.0 0.0 0.0 0.0 5.8045868425688664E-5 6 0.0 0.0 0.0 0.0 8.706880263853299E-5 7 2.9022934212844332E-5 0.0 0.0 0.0 8.706880263853299E-5 8 2.9022934212844332E-5 0.0 0.0 0.0 8.706880263853299E-5 9 2.9022934212844332E-5 0.0 0.0 0.0 8.706880263853299E-5 10-11 2.9022934212844332E-5 0.0 0.0 0.0 8.706880263853299E-5 12-13 2.9022934212844332E-5 0.0 0.0 0.0 3.047408092348655E-4 14-15 2.9022934212844332E-5 0.0 0.0 0.0 4.0632107897982064E-4 16-17 2.9022934212844332E-5 0.0 0.0 0.0 4.933898816183536E-4 18-19 2.9022934212844332E-5 0.0 0.0 2.9022934212844332E-5 6.965504211082639E-4 20-21 2.9022934212844332E-5 0.0 0.0 1.1609173685137733E-4 8.126421579596413E-4 22-23 5.8045868425688664E-5 0.0 0.0 3.627866776605541E-4 8.997109605981743E-4 24-25 5.8045868425688664E-5 0.0 0.0 7.545962895339525E-4 8.997109605981743E-4 26-27 5.8045868425688664E-5 0.0 0.0 0.0013495664408972614 9.577568290238629E-4 28-29 7.255733553211083E-5 0.0 0.0 0.00401967638847894 9.577568290238629E-4 30-31 8.706880263853299E-5 0.0 0.0 0.012363769974671684 9.577568290238629E-4 32-33 1.0158026974495516E-4 0.0 0.0 0.02980655343659113 0.0010158026974495516 34-35 1.1609173685137733E-4 0.0 0.0 0.05528868967546845 0.0011028715000880845 36-37 1.4511467106422167E-4 0.0 0.0 0.09847481578418082 0.0011028715000880845 38-39 1.4511467106422167E-4 0.0 0.0 0.16515500713819067 0.0011464059014073512 40-41 1.4511467106422167E-4 0.0 0.0 0.23913446644673086 0.0011609173685137733 42-43 1.4511467106422167E-4 0.0 0.0 0.31576952423574633 0.0012044517698330398 44-45 1.7413760527706598E-4 0.0 0.0 0.40939751000638214 0.001306032039577995 46-47 2.6120640791559897E-4 0.0 0.0 0.5236462905352438 0.0013785893751101057 48-49 2.9022934212844334E-4 0.0 0.0 0.6421179079920745 0.00140761230932295 50-51 2.9022934212844334E-4 0.0 0.0 0.778119377713463 0.0014511467106422167 52-53 2.9022934212844334E-4 0.0 0.0 0.9099270334410955 0.0015382155132807496 54-55 2.9022934212844334E-4 0.0 0.0 1.0499336680838565 0.0015527269803871718 56-57 2.9022934212844334E-4 0.0 0.0 1.2188616566697168 0.0015962613817064382 58-59 2.9022934212844334E-4 0.0 0.0 1.394305293986361 0.0016252843159192825 60-61 2.9022934212844334E-4 0.0 0.0 1.591269437021829 0.0016252843159192825 62-63 3.4827521055413196E-4 0.0 0.0 1.7810939382409374 0.0018139333883027708 64-65 3.4827521055413196E-4 0.0 0.0 1.9880564821127304 0.001828444855409193 66-67 3.772981447669763E-4 0.0 0.0 2.2340113380994797 0.001842956322515615 68-69 3.772981447669763E-4 0.0 0.0 2.4994550944101537 0.0018719792567284594 70-71 3.772981447669763E-4 0.0 0.0 2.7761162147940928 0.0019735595264734145 72-73 3.918096118733985E-4 0.0 0.0 3.0749218339824314 0.002162208598856903 74-75 4.0632107897982064E-4 0.0 0.0 3.37173938217719 0.0023073232699211245 76-77 4.0632107897982064E-4 0.0 0.0 3.6910932387882225 0.0023798806054532353 78-79 4.0632107897982064E-4 0.0 0.0 4.060148870238752 0.0024089035396660796 80-81 4.3534401319266495E-4 0.0 0.0 4.435560524281893 0.0024089035396660796 82-83 4.933898816183536E-4 0.0 0.0 4.8396033029260055 0.002524995276517457 84-85 4.933898816183536E-4 0.0 0.0 5.260247199939865 0.002539506743623879 86-87 5.224128158311979E-4 0.0 0.0 5.686840798467357 0.00261206407915599 88-89 5.514357500440423E-4 0.0 0.0 6.130848157522555 0.0026846214146881006 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6050 0.0 54.41131 1 GTATCAA 10835 0.0 42.719128 1 TATCAAC 14445 0.0 31.746655 2 ATCAACG 14415 0.0 31.731043 3 TCAACGC 14725 0.0 31.06257 4 AGGTAAG 2680 0.0 30.885378 2 AAGGTAA 2625 0.0 30.445171 1 TAAGGTG 2970 0.0 30.256798 5 CAACGCA 15190 0.0 30.111673 5 GTGTAAG 1360 0.0 29.731613 1 AACGCAG 15810 0.0 28.868456 6 TGGTATC 2825 0.0 26.94263 2 GTAAGGT 3290 0.0 26.591295 4 TAAGGTA 1350 0.0 26.414665 4 GGTAAGG 3445 0.0 25.809303 3 GTGGTAT 3010 0.0 25.286688 1 ACGCAGA 18055 0.0 25.068445 7 CGCAGAG 18325 0.0 24.647257 8 TGTAAGG 1625 0.0 23.126621 2 GTACATG 12490 0.0 22.547466 1 >>END_MODULE