##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3426712 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816629760540135 31.0 31.0 34.0 30.0 34.0 2 32.075088598049675 33.0 31.0 34.0 30.0 34.0 3 32.12434135112609 33.0 31.0 34.0 30.0 34.0 4 35.78747703337777 37.0 35.0 37.0 35.0 37.0 5 35.679284106747225 37.0 35.0 37.0 33.0 37.0 6 35.71609402832803 37.0 35.0 37.0 33.0 37.0 7 35.632662155442304 37.0 35.0 37.0 33.0 37.0 8 35.59551225781449 37.0 35.0 37.0 33.0 37.0 9 37.1425640672458 39.0 37.0 39.0 33.0 39.0 10-11 37.06625972652502 39.0 37.0 39.0 33.0 39.0 12-13 36.94960752464753 39.0 37.0 39.0 33.0 39.0 14-15 37.96643925722383 40.0 37.0 41.0 33.0 41.0 16-17 38.05101581340947 40.0 37.0 41.0 33.0 41.0 18-19 38.18507522663124 40.0 37.5 41.0 33.0 41.0 20-21 38.12253116106635 40.0 37.0 41.0 33.0 41.0 22-23 38.0070993710589 40.0 37.0 41.0 33.0 41.0 24-25 37.877582358832605 40.0 37.0 41.0 32.5 41.0 26-27 37.64629169302819 40.0 37.0 41.0 32.0 41.0 28-29 37.5020353329956 39.5 36.5 41.0 32.0 41.0 30-31 37.21248750989287 39.0 36.0 41.0 31.0 41.0 32-33 37.08204088934232 39.0 36.0 41.0 31.0 41.0 34-35 36.94244190932882 39.0 35.5 41.0 30.5 41.0 36-37 36.791419296398416 39.0 35.0 41.0 30.0 41.0 38-39 36.5518243143865 39.0 35.0 40.0 30.0 41.0 40-41 36.30087500787927 39.0 35.0 40.0 30.0 41.0 42-43 36.00278094569955 38.0 35.0 40.0 29.5 41.0 44-45 35.68440519658495 38.0 35.0 40.0 28.5 41.0 46-47 35.24215107660054 38.0 34.0 40.0 27.0 41.0 48-49 34.77483867917701 38.0 33.5 40.0 26.0 41.0 50-51 34.32255366076869 38.0 33.0 40.0 24.0 41.0 52-53 33.67231226318407 37.0 32.5 40.0 23.0 41.0 54-55 33.19952347906681 36.5 32.0 39.0 22.0 41.0 56-57 32.771529092611225 36.0 31.0 39.0 20.0 40.0 58-59 32.19544435015257 35.5 31.0 39.0 18.5 40.0 60-61 31.66694604040258 35.0 30.0 38.0 16.0 40.0 62-63 31.139375588027242 35.0 29.5 38.0 11.5 40.0 64-65 30.453523085686804 34.0 29.0 37.5 7.5 40.0 66-67 29.783165319991873 34.0 28.0 37.0 6.5 39.0 68-69 29.044383508155924 33.5 26.5 36.0 2.0 39.0 70-71 28.251605328956735 33.0 26.0 35.5 2.0 38.5 72-73 27.68270108488837 32.5 25.5 35.0 2.0 37.5 74-75 26.980088347080233 32.0 24.5 35.0 2.0 37.0 76-77 25.893935060781295 30.5 23.0 34.0 2.0 35.5 78-79 26.38953025524176 31.5 24.0 34.0 2.0 36.0 80-81 26.047266884406977 32.0 23.0 34.0 2.0 36.0 82-83 25.392385908124172 31.0 20.5 34.0 2.0 35.0 84-85 24.639291104709123 31.0 18.5 34.0 2.0 35.0 86-87 23.768865314622296 30.0 11.0 34.0 2.0 35.0 88-89 22.79346411370433 29.5 4.5 34.0 2.0 35.0 90-91 21.951955985796296 29.0 2.0 34.0 2.0 35.0 92-93 20.90387593121336 28.0 2.0 33.0 2.0 35.0 94-95 19.746653789405123 26.5 2.0 33.0 2.0 34.0 96-97 18.380006256726563 24.5 2.0 32.5 2.0 34.0 98-99 16.61321800606529 11.0 2.0 32.0 2.0 34.0 100-101 12.42566168385321 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2738444299744742 1101 2 0.2807172928484789 1101 3 0.27892825489343664 1101 4 0.17826570525920715 1101 5 0.19063831237820494 1101 6 0.23074665453611232 1101 7 0.23825245598341382 1101 8 0.28140014820063897 1101 9 0.33063605232660365 1101 10-11 0.3858727980548906 1101 12-13 0.2979646634197124 1101 14-15 0.407939866490203 1101 16-17 0.3707384996400265 1101 18-19 0.3535802617173758 1101 20-21 0.3952104884066614 1101 22-23 0.4083698580673243 1101 24-25 0.3237363601547827 1101 26-27 0.48857032010913315 1101 28-29 0.43669344401730825 1101 30-31 0.500617945369008 1101 32-33 0.3725696705380628 1101 34-35 0.1675668026742727 1101 36-37 0.3828496288937444 1101 38-39 0.4895148962472291 1101 40-41 0.592260866184823 1101 42-43 0.6222418734435635 1101 44-45 0.6638239990081161 1101 46-47 0.6719161605999986 1101 48-49 0.7293003123467301 1101 50-51 0.7392321865371443 1101 52-53 0.7558704541565859 1101 54-55 0.9138026461651378 1101 56-57 0.9718428918691586 1101 58-59 1.0218007133045788 1101 60-61 1.0268424253261337 1101 62-63 1.1798348025011194 1101 64-65 1.22080697868439 1101 66-67 1.3710191958736395 1101 68-69 1.4906297394601005 1101 70-71 1.671701952154546 1101 72-73 1.8741292419803912 1101 74-75 1.8726075684796086 1101 76-77 1.7324814018609054 1101 78-79 1.842333574073482 1101 80-81 2.0211180391388552 1101 82-83 2.1914724092728584 1101 84-85 2.3955873178986486 1101 86-87 2.436891108025648 1101 88-89 2.190229027608691 1101 90-91 2.502843113745083 1101 92-93 2.834987800199702 1101 94-95 2.599408141430345 1101 96-97 2.857073011783516 1101 98-99 2.976168256826167 1101 100-101 2.606376724468264 1102 1 0.3447099345910871 1102 2 0.2301277418297616 1102 3 0.2360605516437957 1102 4 0.29421280416588047 1102 5 0.2632880794577446 1102 6 0.28419435451591823 1102 7 0.2706479857748221 1102 8 0.28227281391002634 1102 9 0.43149954473532404 1102 10-11 0.35752540330926763 1102 12-13 0.3871797284946652 1102 14-15 0.5528760288250751 1102 16-17 0.460308382806204 1102 18-19 0.3918344512895544 1102 20-21 0.3452360201436946 1102 22-23 0.4635063423554584 1102 24-25 0.347387894204374 1102 26-27 0.3406398888678339 1102 28-29 0.31959172986771023 1102 30-31 0.4323093596034582 1102 32-33 0.38828273931044777 1102 34-35 0.5819131421204276 1102 36-37 0.5759703561885274 1102 38-39 0.6630934415244951 1102 40-41 0.693221362549167 1102 42-43 0.6372131717393259 1102 44-45 0.6505681171839015 1102 46-47 0.5441241296830128 1102 48-49 0.6472045885844011 1102 50-51 0.6984424855645557 1102 52-53 0.7060681239902635 1102 54-55 0.7827214043813342 1102 56-57 0.912100014308308 1102 58-59 0.9057759372551786 1102 60-61 0.8813945648135721 1102 62-63 1.1119069734440927 1102 64-65 1.1402552268428892 1102 66-67 1.128414707170485 1102 68-69 1.2832229834721147 1102 70-71 1.5025209965925335 1102 72-73 1.5689120289474978 1102 74-75 1.6437160545964886 1102 76-77 1.5770973864112925 1102 78-79 1.5800641919618634 1102 80-81 1.6615520712705596 1102 82-83 1.804327634244359 1102 84-85 2.1553049133989965 1102 86-87 2.00821190241869 1102 88-89 1.9955809734950982 1102 90-91 2.3225447096034877 1102 92-93 2.3414892845729085 1102 94-95 2.151218102081323 1102 96-97 2.2659104697731287 1102 98-99 2.3444075249891156 1102 100-101 2.3276136645362815 1103 1 0.19030542402499862 1103 2 0.2360094190996307 1103 3 0.20371432158294311 1103 4 0.1170687208661434 1103 5 0.19186514553209832 1103 6 0.25180795724671867 1103 7 0.2418075778451083 1103 8 0.22942650915226181 1103 9 0.2545182443325231 1103 10-11 0.38384919469910983 1103 12-13 0.3808625541238868 1103 14-15 0.4010550801460795 1103 16-17 0.3220842103211652 1103 18-19 0.32423603458224903 1103 20-21 0.3654558052420853 1103 22-23 0.41944203395022583 1103 24-25 0.3549014523989058 1103 26-27 0.4771004037973441 1103 28-29 0.47173172597783974 1103 30-31 0.3931718764102641 1103 32-33 0.4118436257514091 1103 34-35 0.4497549227820983 1103 36-37 0.5045971027574012 1103 38-39 0.4808220493149591 1103 40-41 0.4648337870418544 1103 42-43 0.5403980437820977 1103 44-45 0.6539946619301631 1103 46-47 0.7764248369077578 1103 48-49 0.6917114409692076 1103 50-51 0.5964285228791866 1103 52-53 0.7932390699914436 1103 54-55 0.9077606907298446 1103 56-57 1.0168863952287523 1103 58-59 1.0158731982743845 1103 60-61 0.8627756543737597 1103 62-63 0.9130704835789203 1103 64-65 1.0236177577434518 1103 66-67 0.9337362280560235 1103 68-69 0.7941632625848882 1103 70-71 1.1649823796974985 1103 72-73 1.2233928219750325 1103 74-75 1.1483832023300664 1103 76-77 1.1774204505632575 1103 78-79 1.256405635782734 1103 80-81 1.4299019671176936 1103 82-83 1.5390625095287014 1103 84-85 1.663466608257874 1103 86-87 1.5155690199070726 1103 88-89 1.4532590058374488 1103 90-91 1.9023458537347047 1103 92-93 2.2869235414231817 1103 94-95 2.005111114960201 1103 96-97 1.8565682113330801 1103 98-99 2.057029295052285 1103 100-101 2.0162503260634246 1104 1 0.23870087664638362 1104 2 0.20045712886756917 1104 3 0.2175379123148744 1104 4 0.16455847849069016 1104 5 0.14027428125550045 1104 6 0.11726565617791351 1104 7 0.1864602614788211 1104 8 0.2250051048569972 1104 9 0.26452295278269844 1104 10-11 0.2925728871481965 1104 12-13 0.2380166218623856 1104 14-15 0.43206339175770125 1104 16-17 0.38143192205096454 1104 18-19 0.3865278993047454 1104 20-21 0.41005540243018146 1104 22-23 0.34285839361994874 1104 24-25 0.3872203823969471 1104 26-27 0.4577655821149378 1104 28-29 0.4754646108778502 1104 30-31 0.3614533070608772 1104 32-33 0.45780654537623633 1104 34-35 0.48412216161191424 1104 36-37 0.4426589121389881 1104 38-39 0.5536375994371738 1104 40-41 0.6382777041132783 1104 42-43 0.5413576897704928 1104 44-45 0.5214608507024465 1104 46-47 0.6477546731527752 1104 48-49 0.6944190907709071 1104 50-51 0.7551320564446797 1104 52-53 0.692329226836705 1104 54-55 0.8103338454479854 1104 56-57 1.0449139353395225 1104 58-59 0.9920455641276149 1104 60-61 1.0264451314797576 1104 62-63 1.0231989218531652 1104 64-65 1.0747556082627945 1104 66-67 1.0729396370936541 1104 68-69 1.1975199536217715 1104 70-71 1.1960540359247744 1104 72-73 1.430000014531693 1104 74-75 1.3086422119793966 1104 76-77 1.4187862968090847 1104 78-79 1.6269925859938645 1104 80-81 1.675337954307782 1104 82-83 1.6628694777024364 1104 84-85 1.9507455796078226 1104 86-87 1.9436339765683819 1104 88-89 2.0024742392033374 1104 90-91 2.4680101957703577 1104 92-93 2.4347394490018885 1104 94-95 2.462291235457954 1104 96-97 2.363846773686298 1104 98-99 2.399757457868894 1104 100-101 2.2284150526557607 1105 1 0.08852501401648638 1105 2 0.1136532144190383 1105 3 0.10117173634721155 1105 4 0.11418641951458142 1105 5 0.07724032106732182 1105 6 -0.045278549641565746 1105 7 0.11700693104024396 1105 8 0.05212712828912203 1105 9 0.14780686077567395 1105 10-11 0.19578131542557742 1105 12-13 0.1729388029688792 1105 14-15 0.2614484617505113 1105 16-17 0.21034299230250753 1105 18-19 0.17825924960440886 1105 20-21 0.14216717574798565 1105 22-23 0.17292060038214174 1105 24-25 0.19117521817096872 1105 26-27 0.12924881784643105 1105 28-29 0.18296226049237418 1105 30-31 0.3684212473546822 1105 32-33 0.2954885721642242 1105 34-35 0.3319996658611828 1105 36-37 0.3694185070913605 1105 38-39 0.3352994372464906 1105 40-41 0.4046901352828485 1105 42-43 0.3677867257409986 1105 44-45 0.33766094663915425 1105 46-47 0.63405419135578 1105 48-49 0.6499655441865215 1105 50-51 0.61449070541493 1105 52-53 0.5439433316472417 1105 54-55 0.5510145590301363 1105 56-57 0.6527367883232316 1105 58-59 0.7595002583661596 1105 60-61 0.7719637485180577 1105 62-63 0.8536658416185645 1105 64-65 1.0191698323217722 1105 66-67 1.0749308202661787 1105 68-69 1.208862444812631 1105 70-71 1.2461966201063568 1105 72-73 1.3591564262808014 1105 74-75 1.3655693727649805 1105 76-77 1.3716377723003923 1105 78-79 1.4084886319666374 1105 80-81 1.4345622966801734 1105 82-83 1.713688077897384 1105 84-85 1.8213887606720398 1105 86-87 1.7306216869983295 1105 88-89 1.6514563765939343 1105 90-91 1.686584357156665 1105 92-93 1.8208962949639016 1105 94-95 1.6297642860789807 1105 96-97 1.747833115513039 1105 98-99 1.8295916067923912 1105 100-101 1.6472075933520127 1106 1 0.00452608098915519 1106 2 0.0493350829297583 1106 3 -0.00940085554077541 1106 4 0.06272826547795773 1106 5 0.049469778068825576 1106 6 0.033759212131620586 1106 7 0.010944752201531571 1106 8 0.028440775473470126 1106 9 -0.2183066283759345 1106 10-11 -0.007623088604759687 1106 12-13 -0.03792041200095042 1106 14-15 -0.09197050620507952 1106 16-17 -0.15426314631334037 1106 18-19 -0.041874952405727583 1106 20-21 -0.07232012375425967 1106 22-23 0.03128579819988886 1106 24-25 -0.006695208819408549 1106 26-27 -0.14877793070535716 1106 28-29 0.09676112356252986 1106 30-31 0.1408215395139436 1106 32-33 -0.0159567228386166 1106 34-35 -0.13556225755836948 1106 36-37 0.00570114538259503 1106 38-39 0.1933455183867352 1106 40-41 0.18789808674566189 1106 42-43 0.17937561538482072 1106 44-45 0.27202923087664743 1106 46-47 -0.197738605823794 1106 48-49 0.012802150156538517 1106 50-51 0.319362983250592 1106 52-53 0.16861666563485045 1106 54-55 0.09575301861352159 1106 56-57 0.21042833382307435 1106 58-59 0.3619889280726909 1106 60-61 0.5274906480565456 1106 62-63 0.4850947294002168 1106 64-65 0.5188491428661806 1106 66-67 0.4485011649688069 1106 68-69 0.41438223539407915 1106 70-71 0.2934490082126331 1106 72-73 0.7574620095172513 1106 74-75 0.6491820000341804 1106 76-77 0.6227402897519383 1106 78-79 0.9459588120937674 1106 80-81 0.9614557013295588 1106 82-83 1.2034084002036565 1106 84-85 1.3290357650959486 1106 86-87 1.1260667014175567 1106 88-89 1.2261383294055612 1106 90-91 1.2167118654231857 1106 92-93 1.2481466573319757 1106 94-95 1.109372588837509 1106 96-97 1.1190249478876062 1106 98-99 1.305784307340474 1106 100-101 1.4452344173971472 1107 1 -0.03058782079141409 1107 2 0.0027299929913340293 1107 3 0.059729486922904584 1107 4 0.0596178823675686 1107 5 0.02457791651029595 1107 6 -0.02197834360593731 1107 7 -0.025645777722331786 1107 8 0.0020728924389246117 1107 9 0.1043851543158496 1107 10-11 0.03842963078128747 1107 12-13 0.0912020437881651 1107 14-15 0.17099215342425111 1107 16-17 0.0781962828127476 1107 18-19 0.047427835230394066 1107 20-21 0.038324005223195456 1107 22-23 0.09479961504703738 1107 24-25 0.136968909844704 1107 26-27 0.18189258829849564 1107 28-29 0.15722931903073345 1107 30-31 0.15813931183171093 1107 32-33 0.1343275007789373 1107 34-35 0.2652139732178682 1107 36-37 0.18131751933230333 1107 38-39 0.2071870872283057 1107 40-41 0.17762159813584333 1107 42-43 0.19870767471687856 1107 44-45 0.14308725860134786 1107 46-47 0.27184188375670004 1107 48-49 0.3301171924715831 1107 50-51 0.43031818087245455 1107 52-53 0.4582459919308448 1107 54-55 0.3445876107814456 1107 56-57 0.44592109931583934 1107 58-59 0.533311766062198 1107 60-61 0.6360097732423391 1107 62-63 0.7272835849224037 1107 64-65 0.8300392723896479 1107 66-67 0.690915852383494 1107 68-69 0.9358641718760197 1107 70-71 0.8045890876860433 1107 72-73 1.1914493301712383 1107 74-75 1.3579346921202067 1107 76-77 1.3891595878379022 1107 78-79 1.6610716522066085 1107 80-81 1.7975387540792767 1107 82-83 1.8046376597662466 1107 84-85 1.938042954103139 1107 86-87 2.018843569194427 1107 88-89 2.066322907923471 1107 90-91 2.0755420592533795 1107 92-93 1.9840666682519874 1107 94-95 1.7529557174206367 1107 96-97 1.7700996589623195 1107 98-99 1.833787310343478 1107 100-101 1.6294965583259557 1108 1 0.04434058564143939 1108 2 0.05915097045302531 1108 3 0.0724834892401276 1108 4 0.07481983816537507 1108 5 0.08110688549005829 1108 6 0.08336985981452472 1108 7 0.08099927120904482 1108 8 0.04861949566117829 1108 9 0.06603381025137622 1108 10-11 0.07254019456696881 1108 12-13 0.04297224483310913 1108 14-15 0.1885860630523979 1108 16-17 0.03245375004001971 1108 18-19 -0.02417269088213203 1108 20-21 0.14456106190781526 1108 22-23 0.10925029381963469 1108 24-25 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57411.0 24 65155.0 25 73996.0 26 84922.0 27 100458.0 28 117432.0 29 137618.0 30 159610.0 31 187567.0 32 219039.0 33 262435.0 34 317943.0 35 382064.0 36 422395.0 37 331303.0 38 88005.0 39 1478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2291248583931 18.07778316491319 14.954010047704852 23.739081928988863 2 16.63684368897441 19.798040410013165 37.76152260918318 25.80359329182924 3 19.864905800015524 22.532032780742455 29.509701323101368 28.093360096140657 4 14.128032936529245 16.196925799425223 34.401052670898515 35.27398859314702 5 14.57643530134806 36.613455153088886 33.19798453440072 15.612125011162338 6 34.68756639017227 34.7936739358312 15.769752462418785 14.749007211577744 7 30.495121854419043 31.97233966554528 18.850986018083805 18.681552461951863 8 28.334545866284028 33.46421685400374 18.577668207210937 19.623569072501297 9 27.08955104101462 16.132961547609145 18.766628583435107 38.01085882794113 10-11 26.01860033758308 25.50816934717595 26.12244624001083 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24.171444505258236 24.5904225135091 27.500783194420592 23.73734978681207 38-39 23.27670971882571 25.089520530693527 27.447466063335206 24.186303687145553 40-41 23.92541096916912 24.903106728309062 27.673045276107334 23.49843702641448 42-43 23.549684223705118 25.085132868853282 27.277057091297664 24.088125816143936 44-45 23.36681950618206 25.260222115348697 27.864383918232416 23.508574460236822 46-47 24.150515239182077 25.78187617012764 26.6655373033518 23.40207128733848 48-49 23.677522359626373 25.849867161290476 26.38907209009686 24.083538388986295 50-51 23.997873179887893 26.042602938326887 26.6125078500907 23.347016031694523 52-53 24.596829263737366 25.313405386854804 26.634394720069853 23.455370629337978 54-55 23.549878133907956 25.864327086723364 26.980411543193593 23.605383236175083 56-57 23.72289000693522 25.95329370447439 26.66871722349547 23.655099065094916 58-59 23.667323078216086 25.34813255388839 26.518598586633484 24.46594578126204 60-61 22.864541869874095 25.897886954024735 27.128352193006005 24.109218983095165 62-63 23.389928888100314 26.005103434429273 27.12425205269658 23.480715624773836 64-65 22.852323155004285 26.046043893141185 26.83159364153677 24.27003931031776 66-67 22.699194935274104 25.986180213448524 27.026005630521194 24.28861922075618 68-69 23.786494394636666 25.38940462229623 27.03073403404205 23.793366949025053 70-71 23.25491167112016 25.856094350544055 26.368626704823768 24.52036727351202 72-73 23.3207984467869 25.98067296757262 26.397560570612626 24.300968015027856 74-75 23.318008633348818 26.527718115791465 26.181336511501403 23.97293673935831 76-77 23.430696831248145 25.82757173640504 26.691694545675272 24.05003688667154 78-79 23.515457383054077 26.216122043521604 26.556871426603696 23.711549146820627 80-81 23.89026079272631 26.220516145826338 26.137827406261287 23.75139565518606 82-83 23.591170330180226 26.406626367142017 26.212195849386315 23.79000745329144 84-85 23.340355042848188 26.608820738032453 26.25310957252206 23.7977146465973 86-87 23.930900700874574 26.700706477836768 25.463319652037487 23.90507316925117 88-89 23.386470762643608 26.929823691048444 25.215206296881675 24.468499249426273 90-91 23.186334027058038 26.790181083888548 25.660703105039396 24.36278178401402 92-93 23.435606776906965 26.217674401230806 26.030044684442387 24.316674137419835 94-95 23.030279330570817 25.975217340361844 26.187374159562065 24.807129169505274 96-97 23.414558912450186 25.504375623046233 26.181949343860822 24.899116120642763 98-99 23.055424558585607 26.07552079077553 25.556612286063142 25.31244236457572 100-101 23.36201583325357 25.56510439161505 25.956748043022 25.11613173210938 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 503.0 1 371.0 2 331.5 3 435.0 4 393.5 5 394.5 6 703.5 7 1632.5 8 2579.5 9 2877.5 10 2494.5 11 2021.0 12 2030.0 13 2233.5 14 2534.5 15 2851.0 16 3134.5 17 3459.0 18 3917.5 19 4470.0 20 5054.5 21 5859.5 22 7045.5 23 8500.5 24 10402.5 25 12879.5 26 16103.0 27 19670.5 28 22494.0 29 25358.5 30 29141.0 31 36816.0 32 50329.5 33 52043.5 34 45139.5 35 45529.0 36 49370.0 37 54761.5 38 60633.0 39 66003.5 40 72405.5 41 81740.5 42 88849.0 43 94550.5 44 101030.5 45 110533.5 46 116841.5 47 123518.0 48 128737.5 49 127100.5 50 128450.0 51 129171.0 52 129956.5 53 137777.5 54 147720.0 55 143531.5 56 132691.5 57 125234.5 58 119709.0 59 108290.5 60 87703.0 61 73216.0 62 64093.5 63 49081.0 64 34611.5 65 26152.5 66 19705.0 67 15123.0 68 11573.5 69 8823.0 70 6875.5 71 5069.0 72 3677.5 73 3023.5 74 2404.5 75 1778.5 76 1288.5 77 887.0 78 599.0 79 407.5 80 242.5 81 118.5 82 72.5 83 40.5 84 23.5 85 13.0 86 7.5 87 5.5 88 4.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02495103177623331 2 0.011176894936020303 3 0.0019260445581653783 4 0.0 5 2.9182493305536034E-4 6 0.0 7 0.0 8 2.9182493305536034E-5 9 2.0427745313875224E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015320808985406417 26-27 0.008565061785174827 28-29 8.754747991660812E-5 30-31 0.0 32-33 2.9182493305536034E-4 34-35 0.00942594533768814 36-37 0.0011818909788742094 38-39 0.011760544802131023 40-41 0.011395763635811823 42-43 0.004567060202316389 44-45 7.003798393328649E-4 46-47 7.295623326384009E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.4591246652768017E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.0213872656937612E-4 66-67 9.046572924716171E-4 68-69 0.0011235259922631373 70-71 4.377373995830406E-5 72-73 2.6264243974982433E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7509495983321623E-4 82-83 0.0011089347456103695 84-85 0.005719768687885063 86-87 0.003968819089552901 88-89 0.0 90-91 0.0011818909788742094 92-93 4.6691989288857655E-4 94-95 4.377373995830406E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3426712.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.07562761410955 #Duplication Level Percentage of deduplicated Percentage of total 1 67.88886655881136 25.849112022372765 2 15.149778709933024 11.536746651911496 3 6.17776481404247 7.056668176410895 4 3.1109916724563105 4.738118417241695 5 1.9116001454811613 3.639268764320917 6 1.2087743784797709 2.7614905862683488 7 0.8221340306443217 2.1912288439790086 8 0.5946006676659734 1.8111834880920412 9 0.4524301874887549 1.5503907006183237 >10 2.3895016625905194 16.5531176209314 >50 0.17573817001832415 4.626704204501342 >100 0.10089655203874166 7.172877197956621 >500 0.00987781038542919 2.647975049604311 >1k 0.006125773881635483 5.371462418016374 >5k 8.422939087248788E-4 2.194347182035999 >10k+ 7.657217352044354E-5 0.2993086757384881 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10266 0.29958747627463295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8668 0.2529538519723864 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7984 0.2329930265513997 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 7881 0.22998722974092947 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 7300 0.21303220113041305 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7294 0.21285710617057985 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7080 0.20661205260319512 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 6514 0.19009476139226175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6058 0.1767875444449373 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 5782 0.16873317629260937 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5572 0.1626048526984468 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 5131 0.1497353731507054 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4946 0.14433661188918123 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4945 0.1443074293958757 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4833 0.14103899014565568 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4760 0.13890866813435154 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4392 0.1281695105979143 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 4391 0.12814032810460874 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4384 0.12793605065146998 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 4254 0.12414232652175029 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3957 0.11547512601000609 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3944 0.11509575359703413 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3828 0.11171058437359194 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3787 0.11051410214806498 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3686 0.10756667032420582 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3514 0.10254728147565363 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4591246652768017E-4 2 0.0 0.0 0.0 0.0 1.4591246652768017E-4 3 0.0 0.0 0.0 0.0 1.4591246652768017E-4 4 0.0 0.0 0.0 0.0 1.4591246652768017E-4 5 0.0 0.0 0.0 0.0 1.4591246652768017E-4 6 2.9182493305536034E-5 0.0 0.0 0.0 1.750949598332162E-4 7 5.836498661107207E-5 0.0 0.0 0.0 1.750949598332162E-4 8 5.836498661107207E-5 0.0 0.0 0.0 1.750949598332162E-4 9 5.836498661107207E-5 0.0 0.0 2.9182493305536034E-5 1.750949598332162E-4 10-11 5.836498661107207E-5 0.0 0.0 2.9182493305536034E-5 1.750949598332162E-4 12-13 5.836498661107207E-5 0.0 0.0 2.9182493305536034E-5 4.6691989288857655E-4 14-15 5.836498661107207E-5 0.0 0.0 4.377373995830405E-5 5.690586194579527E-4 16-17 5.836498661107207E-5 0.0 0.0 1.1672997322214414E-4 5.836498661107207E-4 18-19 5.836498661107207E-5 0.0 0.0 1.6050371318044817E-4 5.982411127634887E-4 20-21 5.836498661107207E-5 0.0 0.0 2.480511930970563E-4 7.149710859856329E-4 22-23 5.836498661107207E-5 0.0 0.0 3.7937241297196847E-4 7.587448259439369E-4 24-25 1.1672997322214414E-4 0.0 0.0 6.566060993745608E-4 7.87927319249473E-4 26-27 1.1672997322214414E-4 0.0 0.0 0.0010797522523048333 8.90066045818849E-4 28-29 1.750949598332162E-4 0.0 0.0 0.0036478116631920047 9.046572924716171E-4 30-31 1.750949598332162E-4 0.0 0.0 0.012460924641463887 9.192485391243851E-4 32-33 1.750949598332162E-4 0.0 0.0 0.03033520179110471 9.338397857771531E-4 34-35 1.750949598332162E-4 0.0 0.0 0.05664321950604545 0.0010213872656937612 36-37 1.750949598332162E-4 0.0 0.0 0.09862223612605903 0.0010213872656937612 38-39 1.750949598332162E-4 0.0 0.0 0.16563983200222254 0.0010797522523048333 40-41 1.750949598332162E-4 0.0 0.0 0.2376038604936744 0.0010797522523048333 42-43 1.750949598332162E-4 0.0 0.0 0.31280714574204077 0.0011089347456103695 44-45 1.750949598332162E-4 0.0 0.0 0.40772320521829675 0.0011672997322214416 46-47 2.1886869979152027E-4 0.0 0.0 0.5220164402494286 0.0012256647188325135 48-49 2.6264243974982433E-4 0.0 0.0 0.6411101954293212 0.0012548472121380494 50-51 2.6264243974982433E-4 0.0 0.0 0.7779760890322851 0.0013132121987491216 52-53 2.772336864025923E-4 0.0 0.0 0.9081154179283231 0.0013715771853601937 54-55 2.9182493305536034E-4 0.0 0.0 1.0509491314122692 0.0013715771853601937 56-57 3.210074263608964E-4 0.0 0.0 1.2166619196477555 0.0014445334186240339 58-59 3.210074263608964E-4 0.0 0.0 1.3903998935422646 0.00154667214519341 60-61 3.210074263608964E-4 0.0 0.0 1.5865354310487723 0.001575854638498946 62-63 3.210074263608964E-4 0.0 0.0 1.7745582354163407 0.0017509495983321622 64-65 3.210074263608964E-4 0.0 0.0 1.971788116421806 0.0017509495983321622 66-67 3.501899196664324E-4 0.0 0.0 2.2173733888345444 0.001780132091637698 68-69 3.501899196664324E-4 0.0 0.0 2.486756984537948 0.0018239058315960024 70-71 3.7937241297196847E-4 0.0 0.0 2.7620062613957637 0.001853088324901538 72-73 3.9396365962473645E-4 0.0 0.0 3.0573768673877466 0.001999000791429218 74-75 4.085549062775045E-4 0.0 0.0 3.350471238901898 0.0020427745313875223 76-77 4.085549062775045E-4 0.0 0.0 3.6691148833050455 0.0020427745313875223 78-79 4.085549062775045E-4 0.0 0.0 4.034786115670065 0.0020427745313875223 80-81 4.6691989288857655E-4 2.9182493305536034E-5 0.0 4.409022409820259 0.0020719570246930585 82-83 4.6691989288857655E-4 2.9182493305536034E-5 0.0 4.804897522756509 0.0021595045046096666 84-85 4.6691989288857655E-4 2.9182493305536034E-5 0.0 5.2124602242616245 0.0021595045046096666 86-87 4.6691989288857655E-4 2.9182493305536034E-5 0.0 5.6312435944427195 0.0022178694912207385 88-89 4.6691989288857655E-4 2.9182493305536034E-5 0.0 6.0694041401786905 0.002290825724484579 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6290 0.0 53.859875 1 GTATCAA 11040 0.0 40.499252 1 TAAGGTG 2675 0.0 32.316322 5 AGGTAAG 2450 0.0 31.993044 2 TATCAAC 14660 0.0 30.395311 2 ATCAACG 14830 0.0 29.946848 3 TCAACGC 14930 0.0 29.682205 4 AAGGTAA 2550 0.0 29.067713 1 CAACGCA 15385 0.0 28.804377 5 GTAAGGT 2875 0.0 28.74655 4 AACGCAG 16080 0.0 27.529873 6 GGTAAGG 3055 0.0 26.58677 3 ACGCAGA 18365 0.0 23.89766 7 CGCAGAG 18555 0.0 23.52496 8 GTACATG 12330 0.0 23.237032 1 GTGTAAG 1325 0.0 22.950415 1 TACATGG 12205 0.0 22.730698 2 AAGGTGT 4030 0.0 22.275688 6 GGTGTGC 4155 0.0 21.948484 8 GTGGTAT 3095 0.0 21.646362 1 >>END_MODULE