FastQCFastQC Report
Thu 26 May 2016
SRR938619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072451
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.24159799194287346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.18297175663019294No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28540.1377113379278931No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27420.13230710882911104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.12714413995795318No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25790.12444202540856213No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25750.12424901722646277No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25320.12217417926889465No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23250.11218600584525279No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21330.10292161310448353No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21270.1026321008313345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.050.443051
GTATCAA59750.045.4553451
ATCAACG77500.034.6133163
TATCAAC78450.034.4973032
TCAACGC77900.034.3745274
CAACGCA81100.033.0181965
AACGCAG84050.031.945476
ACGCAGA93100.028.5304287
GCGTAAA856.3579454E-427.9793661
CGCAGAG94650.027.9126018
TAAGGTG12900.026.1778775
GCAGAGT105900.024.2737569
GTAAGGT13000.023.0495764
AGGTAAG12200.022.6117382
TGGTATC15300.022.382412
GTACATG72400.022.1400241
AGAGTAC94550.022.01089710-11
GTGGTAT17300.021.9953381
TACATGG70500.021.9260312
GAGTACT58300.021.84142112-13
Produced by FastQC (version 0.11.5)