FastQCFastQC Report
Thu 26 May 2016
SRR938619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072451
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51590.2489323028626491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.19484175982930355No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29470.1421987781617032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.14118548520568158No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27600.13317564564855816No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27210.1312938158730894No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26010.1255035704101086No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24170.11662519403353806No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23570.11373007130204768No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA20940.10103978332901477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.054.387461
GTATCAA54500.041.1616741
ATCAACG71900.030.7919223
TATCAAC72600.030.5722792
TCAACGC73400.030.162664
CAACGCA75650.029.2655565
TAAGGTG13600.028.6453885
AACGCAG79000.028.1913536
TGGTATC14450.027.9575182
GTGGTAT16950.025.796791
GTAAGGT15500.024.2144834
ACGCAGA92800.023.845597
ACCGTAT1201.7147636E-423.743258
CGCAGAG93200.023.69238
AGGTAAG14500.023.2722242
GTACATG74900.022.8437941
TACATGG73250.022.709522
TATACCG1503.0300846E-522.1710665
AAGGTAA15000.021.8627781
GGTAAGG15900.021.8125043
Produced by FastQC (version 0.11.5)