##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2072451 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784895276172996 31.0 31.0 34.0 30.0 34.0 2 32.01132475508468 31.0 31.0 34.0 30.0 34.0 3 32.074218401303575 33.0 31.0 34.0 30.0 34.0 4 35.749937151710704 37.0 35.0 37.0 35.0 37.0 5 35.64544734712666 37.0 35.0 37.0 33.0 37.0 6 35.6409550816883 37.0 35.0 37.0 33.0 37.0 7 35.57894203530023 37.0 35.0 37.0 33.0 37.0 8 35.55444447178727 37.0 35.0 37.0 33.0 37.0 9 37.13650021158522 39.0 37.0 39.0 33.0 39.0 10-11 37.03240245487107 39.0 37.0 39.0 33.0 39.0 12-13 36.92712831328702 39.0 37.0 39.0 33.0 39.0 14-15 37.976119821409526 40.0 37.0 41.0 33.0 41.0 16-17 37.88630298134913 40.0 37.0 41.0 33.0 41.0 18-19 38.11509126150631 40.0 37.0 41.0 33.0 41.0 20-21 38.075620123226074 40.0 37.0 41.0 33.0 41.0 22-23 37.97198269102623 40.0 37.0 41.0 33.0 41.0 24-25 37.84980923553802 40.0 37.0 41.0 32.0 41.0 26-27 37.65661721314521 40.0 37.0 41.0 32.0 41.0 28-29 37.54337352246205 39.5 36.5 41.0 32.0 41.0 30-31 37.27957524689366 39.0 36.0 41.0 31.0 41.0 32-33 37.08109552409201 39.0 36.0 41.0 31.0 41.0 34-35 36.992181479803385 39.0 36.0 41.0 31.0 41.0 36-37 36.94044756667347 39.0 36.0 41.0 30.5 41.0 38-39 36.774067275896996 39.0 35.0 41.0 30.0 41.0 40-41 36.59998016840929 39.0 35.0 40.5 30.0 41.0 42-43 36.37169370952558 39.0 35.0 40.0 30.0 41.0 44-45 36.08182219989761 38.5 35.0 40.0 29.5 41.0 46-47 35.710957460514145 38.0 35.0 40.0 28.5 41.0 48-49 35.35895685832862 38.0 34.0 40.0 27.0 41.0 50-51 34.976600411782954 38.0 34.0 40.0 26.0 41.0 52-53 34.47664914634893 38.0 33.0 40.0 24.5 41.0 54-55 34.12691952668604 37.0 33.0 40.0 24.0 41.0 56-57 33.782120301034865 37.0 33.0 40.0 23.0 41.0 58-59 33.2901294168113 36.5 32.0 39.0 22.5 41.0 60-61 32.8808615981753 36.0 31.0 39.0 21.5 40.0 62-63 32.39171903219908 35.5 31.0 39.0 19.5 40.0 64-65 31.860098984246193 35.0 30.5 38.0 18.0 40.0 66-67 31.34255019780926 35.0 30.0 38.0 16.0 40.0 68-69 30.8287399798596 34.0 29.0 37.5 13.0 39.5 70-71 30.24775422917116 34.0 29.0 37.0 8.0 39.0 72-73 29.740885067970243 34.0 28.0 36.0 7.5 39.0 74-75 29.144234531962397 33.5 27.5 36.0 6.5 38.5 76-77 27.952932059672342 31.5 26.0 34.5 2.0 36.5 78-79 28.63019222167376 33.0 27.5 35.0 2.0 37.0 80-81 28.25982351331829 33.0 26.5 35.0 2.0 36.5 82-83 27.815206487391016 33.0 26.0 35.0 2.0 36.0 84-85 27.257203909766744 32.5 26.0 35.0 2.0 36.0 86-87 26.64116594312724 32.0 25.0 34.5 2.0 35.0 88-89 25.879530806759725 31.5 23.5 34.0 2.0 35.0 90-91 25.27016923439927 31.0 20.5 34.0 2.0 35.0 92-93 24.425274469697957 31.0 18.0 34.0 2.0 35.0 94-95 23.598293035637514 30.5 6.0 34.0 2.0 35.0 96-97 22.522427792020174 30.0 2.0 34.0 2.0 35.0 98-99 21.150190764461982 29.0 2.0 34.0 2.0 35.0 100-101 17.236344067965902 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3392476713545385 1101 2 0.3324281400286324 1101 3 0.3512960483345182 1101 4 0.2126899265969584 1101 5 0.14358560281787192 1101 6 0.29865774538253476 1101 7 0.3309854734529267 1101 8 0.31898591641352425 1101 9 0.3925027556943519 1101 10-11 0.33827167796059143 1101 12-13 0.3653688749702937 1101 14-15 0.5511293201945904 1101 16-17 0.5133505498014443 1101 18-19 0.4798948054936716 1101 20-21 0.41746033138642247 1101 22-23 0.44179426648189235 1101 24-25 0.6217017546848496 1101 26-27 0.4119731739532355 1101 28-29 0.47206120528242934 1101 30-31 0.5415097341483772 1101 32-33 0.5244588820901228 1101 34-35 0.6507147311695078 1101 36-37 0.5819339503204475 1101 38-39 0.5834818338149006 1101 40-41 0.6487259205344955 1101 42-43 0.5231302159941222 1101 44-45 0.6965081784329215 1101 46-47 0.5055093529451113 1101 48-49 0.6281247866290371 1101 50-51 0.9027335164830959 1101 52-53 0.7933590479994521 1101 54-55 0.8530058136330538 1101 56-57 0.7382091227822016 1101 58-59 1.0958073586815331 1101 60-61 1.0656210311957395 1101 62-63 1.0442922450440264 1101 64-65 0.9091667632781473 1101 66-67 1.0397706713699044 1101 68-69 1.0410803770824089 1101 70-71 0.9955751680810145 1101 72-73 1.1451769307554223 1101 74-75 1.0592943136028374 1101 76-77 1.1547453153837495 1101 78-79 1.2460694343693781 1101 80-81 1.2026059214913616 1101 82-83 1.398584439744237 1101 84-85 1.5595076012727382 1101 86-87 1.5506024251862804 1101 88-89 1.4940280543625164 1101 90-91 1.4364381171607796 1101 92-93 1.702506775581437 1101 94-95 1.4416314809062243 1101 96-97 1.6275155809532436 1101 98-99 1.8614620308579362 1101 100-101 1.9324369429873798 1102 1 0.21545282387547715 1102 2 0.2822990635837712 1102 3 0.18975292626507922 1102 4 0.1560806857771908 1102 5 0.17976652057773634 1102 6 0.08882949608059931 1102 7 0.10785444769032182 1102 8 0.0804052304644145 1102 9 0.12910778177306526 1102 10-11 0.33246323794162436 1102 12-13 0.24390530613830208 1102 14-15 0.3881110387552553 1102 16-17 0.43526588619046436 1102 18-19 0.3645557822599059 1102 20-21 0.3979408118669028 1102 22-23 0.4033874386013778 1102 24-25 0.4502013068686139 1102 26-27 0.45018902137960737 1102 28-29 0.395846833015419 1102 30-31 0.3171933124635373 1102 32-33 0.38799346930891687 1102 34-35 0.39797514948255497 1102 36-37 0.4630519103857722 1102 38-39 0.529934865794246 1102 40-41 0.41221967744930055 1102 42-43 0.3879819097931829 1102 44-45 0.48112173936227265 1102 46-47 0.4552485658422185 1102 48-49 0.5262729347771824 1102 50-51 0.685898260174163 1102 52-53 0.5267635050881196 1102 54-55 0.4501555949933689 1102 56-57 0.6348952826262604 1102 58-59 0.6265354551991038 1102 60-61 0.6660701645396045 1102 62-63 0.7421677521300509 1102 64-65 0.7216502994458942 1102 66-67 0.6552875039919961 1102 68-69 0.7982651934777536 1102 70-71 0.784673476916165 1102 72-73 0.85362880368098 1102 74-75 0.8173879335385621 1102 76-77 0.7775852605921116 1102 78-79 0.8767922624606292 1102 80-81 0.675980611971319 1102 82-83 0.6554465650274146 1102 84-85 0.6508226004824742 1102 86-87 0.6596522644992753 1102 88-89 0.7460563984961226 1102 90-91 0.8184319531019817 1102 92-93 1.0521656449771513 1102 94-95 1.1822405109889402 1102 96-97 1.2020177146658995 1102 98-99 1.2751783787848083 1102 100-101 1.5647732266394527 1103 1 0.2294368445625743 1103 2 0.2578731786812227 1103 3 0.22889135045812026 1103 4 0.1238405547287229 1103 5 0.1403762977217724 1103 6 0.20015067517734764 1103 7 0.19904729434493618 1103 8 0.19040305198160468 1103 9 0.3229927522220848 1103 10-11 0.24267862482037117 1103 12-13 0.3517663755187357 1103 14-15 0.42561701543699826 1103 16-17 0.4145282151300549 1103 18-19 0.4490519161602151 1103 20-21 0.38905783600279875 1103 22-23 0.2520762465751929 1103 24-25 0.4150647838969306 1103 26-27 0.41767481173034327 1103 28-29 0.5219990240881671 1103 30-31 0.32392821939705385 1103 32-33 0.3859295690325055 1103 34-35 0.4080173894155621 1103 36-37 0.62783362357861 1103 38-39 0.47696130748305166 1103 40-41 0.3848531943976141 1103 42-43 0.6355325312266231 1103 44-45 0.6136215908293465 1103 46-47 0.7959972283386492 1103 48-49 0.7309711017394989 1103 50-51 0.7964534337879243 1103 52-53 0.8573206874327965 1103 54-55 0.8480087992020842 1103 56-57 0.8889240612329914 1103 58-59 1.1120343984934777 1103 60-61 1.1310082616773016 1103 62-63 0.8024880315416922 1103 64-65 0.6403115510267092 1103 66-67 0.5824635819873407 1103 68-69 0.8418946165758925 1103 70-71 0.7024983467023063 1103 72-73 0.6971200204343582 1103 74-75 0.6378565478254465 1103 76-77 0.7438721780955149 1103 78-79 1.0532401755023848 1103 80-81 1.1231803420657833 1103 82-83 1.1628392606028335 1103 84-85 0.9777117270240332 1103 86-87 1.0795014257833948 1103 88-89 1.0778782325523188 1103 90-91 0.9907863696222385 1103 92-93 1.471253751875583 1103 94-95 1.4688524609573932 1103 96-97 1.3103947591154395 1103 98-99 1.5482264440374394 1103 100-101 2.1762079273244055 1104 1 0.1796645257966354 1104 2 0.17382212868770353 1104 3 0.15899785954263734 1104 4 0.13966458709195706 1104 5 0.06661382037624008 1104 6 -0.05479598885661119 1104 7 -0.0033776414150992196 1104 8 0.12113448854807274 1104 9 0.10311870123316424 1104 10-11 0.16234083828501866 1104 12-13 0.2921536541393692 1104 14-15 0.31412513508381323 1104 16-17 0.2921075676889018 1104 18-19 0.23032967233807256 1104 20-21 0.23071573265023915 1104 22-23 0.21689405069808743 1104 24-25 0.2607026068694367 1104 26-27 0.1874962426039346 1104 28-29 0.269099828758641 1104 30-31 0.2385781209082083 1104 32-33 0.3028399981290235 1104 34-35 0.2961950642375015 1104 36-37 0.3524404315562393 1104 38-39 0.34504529067989864 1104 40-41 0.32466202494185126 1104 42-43 0.4566987775430462 1104 44-45 0.37690842440118644 1104 46-47 0.43402342101036595 1104 48-49 0.27449380642021737 1104 50-51 0.3683207727435658 1104 52-53 0.4041444477049154 1104 54-55 0.3218259661839298 1104 56-57 0.3106079592062869 1104 58-59 0.5372659982467667 1104 60-61 0.5905474302455573 1104 62-63 0.4792671919404299 1104 64-65 0.3764713750427937 1104 66-67 0.3956068722271411 1104 68-69 0.45448571790710446 1104 70-71 0.48436269333418025 1104 72-73 0.38844825256812143 1104 74-75 0.44850996684373357 1104 76-77 0.43734558746508867 1104 78-79 0.7157186066833496 1104 80-81 0.5402156209523952 1104 82-83 0.5735055038658636 1104 84-85 0.7688417601160147 1104 86-87 0.8265543939853721 1104 88-89 0.7796137803409522 1104 90-91 0.8499364682467849 1104 92-93 1.2918008382275339 1104 94-95 1.3005556224390595 1104 96-97 1.1690532616914808 1104 98-99 1.0663724110838828 1104 100-101 1.3336119609225676 1105 1 0.1249751464023987 1105 2 0.14766501351793693 1105 3 0.05968057710739316 1105 4 0.06919275397357438 1105 5 0.035104028316879976 1105 6 0.09731146151197834 1105 7 0.12878257707527752 1105 8 0.15793860002640514 1105 9 0.17470451362427042 1105 10-11 0.10831738247232181 1105 12-13 0.24904693583282267 1105 14-15 0.3864854807934677 1105 16-17 0.1835325209542873 1105 18-19 0.23285719923276105 1105 20-21 0.3318417724668947 1105 22-23 0.2459090608879464 1105 24-25 0.23904031377097823 1105 26-27 0.24046602186355415 1105 28-29 0.2867847360253748 1105 30-31 0.4250600667278377 1105 32-33 0.38694446059511733 1105 34-35 0.3236820813213228 1105 36-37 0.2617889093016217 1105 38-39 0.23313738395589212 1105 40-41 0.3127318126957235 1105 42-43 0.4259314221933792 1105 44-45 0.34658231637113346 1105 46-47 0.2571548958527927 1105 48-49 0.5335416459293896 1105 50-51 0.6209097370864995 1105 52-53 0.49580490578512126 1105 54-55 0.46050374151933937 1105 56-57 0.34630230611811186 1105 58-59 0.496574630808027 1105 60-61 0.41438276102542915 1105 62-63 0.2607285148783447 1105 64-65 0.595556342871614 1105 66-67 0.39518572581408407 1105 68-69 0.4467381152644201 1105 70-71 0.3870616615092537 1105 72-73 0.38805920276390893 1105 74-75 0.40977065679762603 1105 76-77 0.3074194874753928 1105 78-79 0.40855924734463045 1105 80-81 0.43195732560004885 1105 82-83 0.6375904360317861 1105 84-85 0.7014609276920858 1105 86-87 0.7089952146470289 1105 88-89 0.839713545679075 1105 90-91 0.7703962084004239 1105 92-93 1.0226639646706666 1105 94-95 1.2578110446586024 1105 96-97 1.3399343103622599 1105 98-99 1.4766063013420094 1105 100-101 1.6021208432538039 1106 1 0.02858157507489878 1106 2 0.07804823120584814 1106 3 0.058345128444244665 1106 4 0.0036586103724118857 1106 5 -0.06334488196875299 1106 6 -0.11262589368671883 1106 7 0.07893017414798464 1106 8 0.11276417986739062 1106 9 -0.24522171066198695 1106 10-11 0.10353800211267128 1106 12-13 0.039741766903134135 1106 14-15 -0.014040221702742883 1106 16-17 0.0930826090821597 1106 18-19 0.049213771850716626 1106 20-21 0.01580373826297432 1106 22-23 0.14936714260202422 1106 24-25 0.1678148635624126 1106 26-27 0.06864959649707458 1106 28-29 0.1834739579504756 1106 30-31 0.15433730078412822 1106 32-33 0.1541756934007239 1106 34-35 0.24108119071791378 1106 36-37 0.2888718473147591 1106 38-39 0.23704265839123906 1106 40-41 0.4811747304163063 1106 42-43 0.24314677698388465 1106 44-45 0.10683211308689522 1106 46-47 -0.6664545714870798 1106 48-49 -0.2099491829682023 1106 50-51 0.07968656261809315 1106 52-53 -0.005531520989840999 1106 54-55 0.3461654048180378 1106 56-57 0.24579675272526913 1106 58-59 -0.03607311135126423 1106 60-61 0.011455794026268507 1106 62-63 -0.06746549294785353 1106 64-65 0.18891124683081628 1106 66-67 0.07694465776814852 1106 68-69 -0.15523264962553895 1106 70-71 -0.18489867670876592 1106 72-73 0.27628123675394534 1106 74-75 0.3387998416979272 1106 76-77 0.27409456590365977 1106 78-79 0.5249301003950642 1106 80-81 -0.15603198298575194 1106 82-83 0.4443057605008178 1106 84-85 0.6641743272370668 1106 86-87 0.9514440284256267 1106 88-89 0.8588343274903707 1106 90-91 0.9920487348306359 1106 92-93 1.1806449984305196 1106 94-95 1.1296474235060856 1106 96-97 1.0372677167279463 1106 98-99 1.1087241025205685 1106 100-101 0.9950400962111274 1107 1 0.12492123325502291 1107 2 0.09338946154826289 1107 3 0.052786147168156106 1107 4 0.0247518759844354 1107 5 0.0699387907649367 1107 6 -0.0015787811034329025 1107 7 -0.02692691890340626 1107 8 0.0517925636731178 1107 9 0.12200826300265533 1107 10-11 0.1089503935965368 1107 12-13 0.16612809884973245 1107 14-15 0.31688787478699965 1107 16-17 0.20775138918202174 1107 18-19 0.11949929066321374 1107 20-21 0.19243194865880753 1107 22-23 0.21860642307793654 1107 24-25 0.22276634922085492 1107 26-27 0.20492528860728498 1107 28-29 0.09981987345081933 1107 30-31 0.11039931260571478 1107 32-33 0.12263348623050518 1107 34-35 0.26815330887372824 1107 36-37 0.32884937091889554 1107 38-39 0.31075784913684146 1107 40-41 0.23577537431064144 1107 42-43 0.2780802358023138 1107 44-45 0.3247581077859536 1107 46-47 0.17801143629816352 1107 48-49 0.3867045054743983 1107 50-51 0.2907598814342762 1107 52-53 0.3228831691489731 1107 54-55 0.17080019513748113 1107 56-57 0.17969777487419236 1107 58-59 0.3640094869692092 1107 60-61 0.3697643235200232 1107 62-63 0.46402760399595167 1107 64-65 0.21671271535359082 1107 66-67 0.3211716130590041 1107 68-69 0.5005392731689504 1107 70-71 0.6341658073470242 1107 72-73 0.6691588144851686 1107 74-75 0.5983291836822815 1107 76-77 0.6156535446395672 1107 78-79 0.8494116329389527 1107 80-81 0.7993830227709395 1107 82-83 0.8540189377271368 1107 84-85 0.9226187989179309 1107 86-87 1.1358932540610773 1107 88-89 0.971825527657515 1107 90-91 1.3309747068797186 1107 92-93 1.3919859891488997 1107 94-95 1.4674017356273978 1107 96-97 1.5498218358799392 1107 98-99 1.538242142802389 1107 100-101 1.4361435987651738 1108 1 0.12226092357734686 1108 2 0.17333614362046035 1108 3 0.20726184869369746 1108 4 0.1874408723956975 1108 5 0.1105254286731352 1108 6 0.1779382207108 1108 7 0.13734146179420748 1108 8 0.10170797670911469 1108 9 0.18285376847183699 1108 10-11 0.10720237336187921 1108 12-13 0.19698909813463672 1108 14-15 0.3356012921665581 1108 16-17 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62-63 -0.9761265139639477 2316 64-65 -1.0114488731051203 2316 66-67 -1.0906834341879375 2316 68-69 -0.9421590864475107 2316 70-71 -0.9493407021460563 2316 72-73 -0.905950960621432 2316 74-75 -0.9807737627722943 2316 76-77 -1.0230798126488487 2316 78-79 -0.9492109082138676 2316 80-81 -0.9448942758013672 2316 82-83 -1.0316030091154111 2316 84-85 -1.0502826869407862 2316 86-87 -1.2171757264092982 2316 88-89 -1.1195357168776 2316 90-91 -0.8890824241273982 2316 92-93 -1.3668800386186533 2316 94-95 -1.2802169235147929 2316 96-97 -1.490458201958301 2316 98-99 -1.409402194363139 2316 100-101 -1.952415752031289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 31.0 5 82.0 6 145.0 7 388.0 8 937.0 9 1796.0 10 2762.0 11 4355.0 12 6127.0 13 8500.0 14 11234.0 15 14370.0 16 17953.0 17 21862.0 18 21046.0 19 19159.0 20 19280.0 21 20427.0 22 22125.0 23 25137.0 24 29079.0 25 33725.0 26 39866.0 27 47917.0 28 57127.0 29 67720.0 30 79977.0 31 95259.0 32 113904.0 33 139304.0 34 176294.0 35 227649.0 36 291733.0 37 312767.0 38 137457.0 39 4941.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.141622572062474 19.068073447794337 15.344150223070605 23.446153757072587 2 16.109849494259 20.143129144507913 38.906911986776194 24.840109374456894 3 19.37705644186521 23.525815568136473 29.743091634012092 27.35403635598622 4 13.62059398296702 16.912687881492918 35.91092668098145 33.555791454558616 5 14.15047933239586 37.10422311657053 33.94254576454953 14.802751786484079 6 33.27478746159736 35.855261094482906 16.71983243013459 14.15011901378514 7 29.54067430303539 32.088671819020085 19.88418544033128 18.48646843761324 8 27.093282301969985 34.692882968041225 19.12851015536676 19.085324574622028 9 26.45346017831061 16.55112714365744 19.49479143294582 37.50062124508613 10-11 25.251139833945413 26.225807027524418 27.063655546017735 21.459397592512442 12-13 26.287135377386484 23.320575492496566 27.26896799972593 23.12332113039102 14-15 22.81496643346453 24.922953546308214 25.39927361370667 26.86280640652059 16-17 22.746617410978594 27.99188014577908 26.319271239706026 22.942231203536295 18-19 22.78533967751228 26.484824007901754 28.39010427749558 22.339732037090382 20-21 23.11912320243036 25.70198282130675 28.74509457642183 22.43379939984106 22-23 22.95702045548966 25.615635785840052 28.64970028241922 22.777643476251068 24-25 22.98032762757727 25.869984039646337 28.551974897729483 22.59771343504691 26-27 22.425662526714106 26.28935821969333 28.35000557322958 22.93497368036298 28-29 22.55691757841027 26.6419648063631 28.158238914018852 22.642878701207774 30-31 22.6697381042912 26.09292096034217 28.132340423889275 23.105000511477357 32-33 22.336041249887813 26.088981018527903 28.146722562078942 23.428255169505345 34-35 23.207383594997722 26.195658704415635 27.932688467970635 22.66426923261601 36-37 23.387488130386572 25.654243439587738 27.927103983243185 23.03116444678251 38-39 22.661066933093927 26.155415527366017 27.84706135829167 23.336456181248387 40-41 23.198664368234837 25.872461159678682 28.047832795115873 22.881041676970607 42-43 22.98493476589331 25.922080002586345 27.777326078370677 23.315659153149664 44-45 22.873480850940624 26.180587223344165 28.04851625854427 22.89741566717094 46-47 23.35102253322274 26.441831435339125 27.190003527224526 23.01714250421361 48-49 23.0114970148872 26.452543389445637 27.17946045527735 23.356499140389808 50-51 23.267070183802314 26.578884640549997 27.122180382196092 23.031864793451597 52-53 23.628134030671895 26.221488469449937 27.161486568319347 22.988890931558817 54-55 23.073408063677935 26.470364002819878 27.353916587733522 23.102311345768662 56-57 23.15043877997598 26.551628964930895 27.17053382685525 23.127398428237868 58-59 23.10236311297609 26.21065289119188 27.0674855567699 23.619498439062127 60-61 22.717979821959602 26.559590552442497 27.363445504863567 23.358984120734338 62-63 22.78844736319541 26.60020658489971 27.428934866883996 23.182411185020882 64-65 22.523012938979548 26.581552151521887 27.225062670429907 23.67037223906866 66-67 22.540860497059946 26.573045595395122 27.23541740908945 23.65067649845548 68-69 23.035683002856512 26.191215028953618 27.38142866174382 23.391673306446048 70-71 22.790567393801393 26.289856520026184 26.984445218086133 23.93513086808629 72-73 22.824844990228957 26.425317860503267 26.9708798764747 23.778957272793072 74-75 22.832542420326522 26.760417910952008 26.76287933358189 23.64416033513958 76-77 22.88783823807355 26.273809515188773 27.094480607333782 23.743871639403896 78-79 22.92308765314739 26.529869035405834 27.0448028894409 23.502240422005872 80-81 23.15369391532074 26.627678307911022 26.729806764369236 23.488821012399004 82-83 23.01873012792943 26.702318736065113 26.802985390181632 23.475965745823817 84-85 22.981572987291766 26.688402849061422 26.77261607792209 23.557408085724724 86-87 23.171632103724534 26.64557212470648 26.4616423244596 23.721153447109387 88-89 22.898617284522974 26.743968729535982 26.31592672651093 24.04148725943011 90-91 22.876582908509548 26.708213930198184 26.505094868933064 23.910108292359205 92-93 22.970860136716258 26.338886139205208 26.715342100735274 23.97491162334326 94-95 22.729660730837907 26.19885183209016 26.746872872584444 24.324614564487494 96-97 22.912752251264017 25.871646032342515 26.82117007563224 24.394431640761233 98-99 22.620342787181364 26.134607804065336 26.47211821699298 24.77293119176032 100-101 22.820855889169458 25.829316816046926 26.834469620651756 24.51535767413186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 153.0 2 130.5 3 170.5 4 161.0 5 163.0 6 266.0 7 629.5 8 1007.5 9 1130.0 10 981.5 11 815.0 12 900.5 13 1043.5 14 1154.5 15 1266.0 16 1433.0 17 1633.0 18 1911.5 19 2195.0 20 2461.0 21 2968.0 22 3623.0 23 4490.5 24 5518.5 25 6883.0 26 9004.5 27 11646.0 28 14169.0 29 16920.5 30 20109.0 31 24546.5 32 31816.5 33 34636.5 34 34237.5 35 36888.0 36 40532.5 37 44498.5 38 48443.0 39 51722.5 40 55013.0 41 59751.5 42 63479.5 43 66602.5 44 69673.0 45 74059.5 46 77174.0 47 79249.0 48 80570.5 49 79348.0 50 78859.0 51 77885.0 52 76429.0 53 76964.0 54 79159.0 55 75639.5 56 68809.5 57 63199.5 58 58865.5 59 52383.0 60 42041.5 61 35124.0 62 30403.5 63 23029.0 64 16349.5 65 12535.0 66 9475.5 67 7254.5 68 5372.5 69 3958.0 70 3008.0 71 2079.0 72 1541.0 73 1318.0 74 1035.0 75 781.0 76 581.5 77 411.0 78 265.5 79 166.0 80 93.5 81 59.5 82 40.5 83 22.5 84 16.0 85 6.0 86 3.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.272765918229189E-4 2 0.04994086711820931 3 0.0 4 4.825204552483991E-5 5 0.04458489006495208 6 9.650409104967982E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006200387849941928 26-27 0.0021230900030929562 28-29 1.2063011381209977E-4 30-31 0.0011097970470713178 32-33 4.3426840972355925E-4 34-35 0.0113633567210998 36-37 0.0221235628731391 38-39 0.004366810119998012 40-41 0.001133923069833738 42-43 0.0014716873885076173 44-45 0.007527319101875027 46-47 0.0 48-49 0.0 50-51 3.377643186738794E-4 52-53 0.0 54-55 0.0010615450015464781 56-57 0.0 58-59 0.022171814918663938 60-61 0.0 62-63 0.008661242171708765 64-65 0.014547991725739234 66-67 0.006055631713367409 68-69 0.016598703660544927 70-71 7.237806828725987E-5 72-73 4.825204552483991E-5 74-75 0.0230886037836359 76-77 0.05001324518649657 78-79 0.030471166748936407 80-81 0.02656275106142437 82-83 0.03676805868992801 84-85 0.01592317502319717 86-87 0.009312644786294103 88-89 0.08410331534979597 90-91 0.024777425377005297 92-93 0.023908888557558176 94-95 0.026128482651700813 96-97 0.03770897357766239 98-99 0.0534391404187602 100-101 0.013438194678667916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2072451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.770432180669346 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27446878778902 40.47540718889721 2 13.857236772520414 14.902192201765729 3 4.668218046643726 7.530363056448996 4 2.09700833980233 4.510281788705569 5 1.1025142643658146 2.9641334240151296 6 0.6740686838984775 2.174697867160568 7 0.43407119798596183 1.6338137139020241 8 0.31532348297729107 1.3564063965122304 9 0.23240287806657675 1.1246762874304108 >10 1.2207498581373588 11.803639090119304 >50 0.07502567613071223 2.7294698086988047 >100 0.04236289747919279 4.282109451847931 >500 0.003319414048227142 1.269230749986272 >1k 0.0031399862618364796 2.9947111458761606 >5k 8.971389319532798E-5 0.24886782863361973 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5159 0.2489323028626491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.19484175982930355 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2947 0.1421987781617032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2926 0.14118548520568158 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2760 0.13317564564855816 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2721 0.1312938158730894 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2601 0.1255035704101086 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2417 0.11662519403353806 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2357 0.11373007130204768 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2094 0.10103978332901477 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.825204552483991E-5 2 0.0 0.0 0.0 0.0 4.825204552483991E-5 3 0.0 0.0 0.0 0.0 4.825204552483991E-5 4 0.0 0.0 0.0 0.0 4.825204552483991E-5 5 0.0 0.0 0.0 0.0 4.825204552483991E-5 6 0.0 0.0 0.0 0.0 9.650409104967982E-5 7 0.0 0.0 0.0 0.0 9.650409104967982E-5 8 0.0 0.0 0.0 0.0 9.650409104967982E-5 9 0.0 0.0 0.0 0.0 9.650409104967982E-5 10-11 0.0 0.0 0.0 0.0 1.4475613657451974E-4 12-13 0.0 0.0 0.0 2.4126022762419956E-5 4.342684097235592E-4 14-15 0.0 0.0 0.0 4.825204552483991E-5 5.307725007732391E-4 16-17 0.0 0.0 0.0 4.825204552483991E-5 5.79024546298079E-4 18-19 0.0 0.0 0.0 4.825204552483991E-5 6.755286373477588E-4 20-21 0.0 0.0 0.0 7.237806828725987E-5 7.720327283974386E-4 22-23 0.0 0.0 0.0 2.4126022762419958E-4 7.720327283974386E-4 24-25 0.0 0.0 0.0 5.066464780108191E-4 7.720327283974386E-4 26-27 0.0 0.0 0.0 0.001158049092596158 8.202847739222785E-4 28-29 0.0 0.0 0.0 0.0032087610274018543 8.202847739222785E-4 30-31 0.0 0.0 0.0 0.011604616948724 8.202847739222785E-4 32-33 0.0 0.0 0.0 0.02796206038164473 8.685368194471184E-4 34-35 0.0 0.0 0.0 0.05295661996351181 0.001109797047071318 36-37 0.0 0.0 0.0 0.094791143433548 0.001109797047071318 38-39 0.0 0.0 0.0 0.15838733943528702 0.001109797047071318 40-41 0.0 0.0 0.0 0.22818392328696796 0.001109797047071318 42-43 0.0 0.0 0.0 0.30174416668958637 0.001109797047071318 44-45 0.0 0.0 0.0 0.39721083876048213 0.001133923069833738 46-47 0.0 0.0 0.0 0.5090832063098235 0.0012545531836458377 48-49 0.0 0.0 0.0 0.6238506965906552 0.0014475613657451974 50-51 0.0 0.0 0.0 0.7525630280281657 0.0015440654567948772 52-53 0.0 0.0 0.0 0.8805033267372787 0.001640569547844557 54-55 0.0 0.0 0.0 1.0170324895498133 0.001640569547844557 56-57 0.0 0.0 0.0 1.1742376538697417 0.001664695570606977 58-59 0.0 0.0 0.0 1.3440607280944157 0.0016888215933693969 60-61 0.0 0.0 0.0 1.5327744781420647 0.0017129476161318168 62-63 0.0 0.0 0.0 1.7257102821731372 0.0017853256844190767 64-65 0.0 0.0 0.0 1.9294062923562487 0.0017853256844190767 66-67 0.0 0.0 0.0 2.159447919395923 0.0017853256844190767 68-69 0.0 0.0 0.0 2.416824330225419 0.0017853256844190767 70-71 0.0 0.0 0.0 2.6871564152783347 0.0017853256844190767 72-73 0.0 0.0 0.0 2.9740148259235077 0.0018818297754687567 74-75 0.0 0.0 0.0 3.2672183805551978 0.0020265859120432764 76-77 0.0 0.0 0.0 3.5802535258975965 0.0020265859120432764 78-79 0.0 0.0 0.0 3.9347130523230707 0.0020507119348056966 80-81 0.0 0.0 0.0 4.3041307128612445 0.002171342048617796 82-83 0.0 0.0 0.0 4.6930663258142165 0.0024126022762419957 84-85 0.0 0.0 0.0 5.097321963221326 0.0024126022762419957 86-87 0.0 0.0 0.0 5.506161544953295 0.0025091063672916755 88-89 0.0 0.0 0.0 5.943204447294532 0.0026056104583413553 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 54.38746 1 GTATCAA 5450 0.0 41.161674 1 ATCAACG 7190 0.0 30.791922 3 TATCAAC 7260 0.0 30.572279 2 TCAACGC 7340 0.0 30.16266 4 CAACGCA 7565 0.0 29.265556 5 TAAGGTG 1360 0.0 28.645388 5 AACGCAG 7900 0.0 28.191353 6 TGGTATC 1445 0.0 27.957518 2 GTGGTAT 1695 0.0 25.79679 1 GTAAGGT 1550 0.0 24.214483 4 ACGCAGA 9280 0.0 23.84559 7 ACCGTAT 120 1.7147636E-4 23.74325 8 CGCAGAG 9320 0.0 23.6923 8 AGGTAAG 1450 0.0 23.272224 2 GTACATG 7490 0.0 22.843794 1 TACATGG 7325 0.0 22.70952 2 TATACCG 150 3.0300846E-5 22.171066 5 AAGGTAA 1500 0.0 21.862778 1 GGTAAGG 1590 0.0 21.812504 3 >>END_MODULE