FastQCFastQC Report
Thu 26 May 2016
SRR938618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065645
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.2576434963413365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.19282112850949704No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28100.13603499149176165No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28100.13603499149176165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.13526041502775163No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26130.12649801877863814No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25170.12185055999457796No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24860.12034981809555853No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22120.10708519614938675No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22030.10664949688838112No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21130.10229250427832469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.050.86191
GTATCAA59150.043.5530471
TATCAAC77150.033.2044832
ATCAACG76850.033.0867273
TCAACGC78150.032.475524
CAACGCA79650.031.8623875
AACGCAG83450.030.4632766
ACGCAGA93450.026.9941547
CGCAGAG95450.026.3787618
GTACATG71500.024.0423661
TAAGGTG13450.024.0275485
TACATGG70350.023.9156972
GCAGAGT104250.023.5135179
ACATGGG73150.022.3505383
AGAGTAC92900.021.83488510-11
AGGTAAG13700.021.5087432
AAGGTAA12950.021.3272191
GAGTACT59400.020.59147512-13
GTAAGGT15050.020.5267754
AGTACTT62950.020.2602712-13
Produced by FastQC (version 0.11.5)