##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938618_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065645 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08025725620811 31.0 31.0 34.0 28.0 34.0 2 31.177088996415165 31.0 31.0 34.0 27.0 34.0 3 31.246012746623936 31.0 31.0 34.0 27.0 34.0 4 34.875862018885144 37.0 35.0 37.0 32.0 37.0 5 34.887912492223975 37.0 35.0 37.0 32.0 37.0 6 34.918698517896345 37.0 35.0 37.0 32.0 37.0 7 34.84836310208192 37.0 35.0 37.0 32.0 37.0 8 34.80602426844884 37.0 35.0 37.0 32.0 37.0 9 36.31901512602601 39.0 35.0 39.0 32.0 39.0 10-11 36.1639981700631 39.0 35.0 39.0 31.0 39.0 12-13 36.02226011729992 38.5 35.0 39.0 30.0 39.0 14-15 37.02073928482387 39.0 36.0 41.0 31.0 41.0 16-17 36.871772255155165 39.0 36.0 41.0 31.0 41.0 18-19 36.85950998356446 39.0 36.0 41.0 30.5 41.0 20-21 36.937825715454494 39.0 36.0 41.0 30.5 41.0 22-23 36.860171278220605 39.0 36.0 41.0 30.0 41.0 24-25 36.73784144903892 39.0 36.0 41.0 30.0 41.0 26-27 36.54640560212428 39.0 36.0 41.0 30.0 41.0 28-29 36.371000825408046 39.0 35.5 41.0 29.5 41.0 30-31 36.14536379678018 39.0 35.0 40.0 29.0 41.0 32-33 35.86896247903197 38.0 35.0 40.0 27.5 41.0 34-35 35.61858015293044 38.0 34.5 40.0 27.0 41.0 36-37 35.32127834163179 38.0 34.0 40.0 26.0 41.0 38-39 35.05579443708866 38.0 34.0 40.0 25.0 41.0 40-41 34.9942744276001 38.0 34.0 40.0 25.0 41.0 42-43 34.8138155394562 38.0 33.5 40.0 24.5 41.0 44-45 34.530707115695094 38.0 33.0 40.0 24.0 41.0 46-47 34.175106564777586 38.0 33.0 40.0 23.0 41.0 48-49 33.80904100172101 38.0 33.0 40.0 21.5 41.0 50-51 33.053055341067804 37.0 31.5 39.5 20.0 40.5 52-53 33.10589936799401 37.0 32.0 39.5 20.0 40.0 54-55 33.229926971962755 37.0 32.0 40.0 19.0 41.0 56-57 32.91949633165428 37.0 32.0 40.0 17.5 41.0 58-59 32.59253477727296 36.5 31.0 39.5 14.5 41.0 60-61 32.136575984740844 36.0 31.0 39.0 11.0 41.0 62-63 31.611874257193275 35.5 30.5 39.0 8.0 40.0 64-65 31.12166926069097 35.0 29.5 38.5 7.0 40.0 66-67 30.57611980761457 35.0 29.0 38.0 2.0 40.0 68-69 30.016651457535055 34.5 28.5 37.5 2.0 40.0 70-71 29.390710165589923 34.0 28.0 37.0 2.0 39.0 72-73 28.85134159064118 34.0 26.5 36.0 2.0 39.0 74-75 28.247364624608778 34.0 26.0 36.0 2.0 38.5 76-77 27.52365968014833 33.0 25.5 35.0 2.0 37.5 78-79 26.7628067746394 32.0 24.0 35.0 2.0 37.0 80-81 26.128612370470243 32.0 22.5 35.0 2.0 36.0 82-83 25.482490214920762 31.5 20.0 35.0 2.0 36.0 84-85 24.840614190724928 31.0 18.0 34.0 2.0 35.5 86-87 24.198036932774023 31.0 13.0 34.0 2.0 35.0 88-89 23.415568260761166 30.5 6.0 34.0 2.0 35.0 90-91 22.59335873298655 29.5 2.0 34.0 2.0 35.0 92-93 21.767258410811152 29.0 2.0 34.0 2.0 35.0 94-95 20.82307511697315 29.0 2.0 34.0 2.0 35.0 96-97 19.61673738711153 26.5 2.0 33.5 2.0 35.0 98-99 18.149704813750667 24.5 2.0 33.0 2.0 34.5 100-101 14.727569354850424 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7187250377618568 1101 2 0.6069768943263085 1101 3 0.7326991298541898 1101 4 0.595933766126926 1101 5 0.6111990101390319 1101 6 0.591404617026491 1101 7 0.48263410390362793 1101 8 0.6622796472416894 1101 9 0.6254459553994209 1101 10-11 0.6723806364810585 1101 12-13 0.7119194606424699 1101 14-15 0.9251066647015804 1101 16-17 0.8278302131254733 1101 18-19 0.7409889282009772 1101 20-21 0.8955647340746324 1101 22-23 1.0262844645052809 1101 24-25 1.0342827244526802 1101 26-27 0.9677102090198701 1101 28-29 1.182490609105912 1101 30-31 0.969359907935754 1101 32-33 0.9701200137383523 1101 34-35 0.9702993068613424 1101 36-37 0.9819405385248174 1101 38-39 1.0736981831206336 1101 40-41 1.0088787111543098 1101 42-43 1.041896007524997 1101 44-45 1.0191486265604865 1101 46-47 1.0223680484741067 1101 48-49 1.0133335768042926 1101 50-51 1.0016855673301421 1101 52-53 1.154040105141128 1101 54-55 1.1594357280214425 1101 56-57 0.9697927905645187 1101 58-59 1.0567934850953549 1101 60-61 1.1145840768279456 1101 62-63 1.1367387750147948 1101 64-65 0.954290770433257 1101 66-67 0.9685109960965974 1101 68-69 0.955864152159851 1101 70-71 0.8605359937768213 1101 72-73 0.8501434837994495 1101 74-75 0.8062639253025203 1101 76-77 1.0160933746688592 1101 78-79 0.9341492723849107 1101 80-81 1.2393345588841562 1101 82-83 1.2310499969748392 1101 84-85 1.1615350982413766 1101 86-87 1.2429525693541095 1101 88-89 1.3597089520653611 1101 90-91 1.3713575944045928 1101 92-93 1.5471454510673013 1101 94-95 1.4095317836507064 1101 96-97 1.245749221959489 1101 98-99 1.149073056466932 1101 100-101 0.8373077585452062 1102 1 0.5997193677743482 1102 2 0.6247153837468922 1102 3 0.5515560852233037 1102 4 0.5300916307713806 1102 5 0.5140913735657193 1102 6 0.5875023143767066 1102 7 0.6286593368526567 1102 8 0.5180187016225091 1102 9 0.6707228587184133 1102 10-11 0.6894354948963937 1102 12-13 0.6736569948725517 1102 14-15 0.7444841697711695 1102 16-17 0.8596004898103828 1102 18-19 0.8307057297494822 1102 20-21 0.7924701692444742 1102 22-23 0.8447007120626964 1102 24-25 0.7327145715134833 1102 26-27 0.7995692582364455 1102 28-29 0.6736378666705107 1102 30-31 0.734494435451353 1102 32-33 0.7929358163167919 1102 34-35 0.9902129417203653 1102 36-37 0.8514917543056555 1102 38-39 1.006519120818723 1102 40-41 0.9830096965051354 1102 42-43 1.0318089920002436 1102 44-45 1.0471197398136596 1102 46-47 0.9933303543661012 1102 48-49 0.803744493156195 1102 50-51 1.084300323664344 1102 52-53 1.0336693572677333 1102 54-55 1.172067491406004 1102 56-57 1.1479236846674752 1102 58-59 1.1254810494663587 1102 60-61 1.1831073464916173 1102 62-63 1.1377357065623848 1102 64-65 1.1112936563899218 1102 66-67 1.0625810006881125 1102 68-69 0.8426202029130785 1102 70-71 0.9070692541118532 1102 72-73 0.8844776801046841 1102 74-75 1.0015843655055576 1102 76-77 0.9409599731908358 1102 78-79 0.8354598442275609 1102 80-81 0.9053671013702314 1102 82-83 1.0795316906130168 1102 84-85 1.094481744445222 1102 86-87 0.9972239890642314 1102 88-89 1.2693383692147222 1102 90-91 1.2852162048548053 1102 92-93 1.2513987182750483 1102 94-95 1.1318744563009062 1102 96-97 1.4149353977126005 1102 98-99 1.575378265070249 1102 100-101 1.2757615536316056 1103 1 0.6523195061130274 1103 2 0.6604176240331725 1103 3 0.5857463180552998 1103 4 0.5581795272803305 1103 5 0.5358468456607497 1103 6 0.5358582563659269 1103 7 0.460630720182273 1103 8 0.4639365673196636 1103 9 0.5883813336773969 1103 10-11 0.6292060070353216 1103 12-13 0.7184248488509368 1103 14-15 0.883835378519656 1103 16-17 0.8433649882183474 1103 18-19 0.7743755919252777 1103 20-21 0.7964023890086125 1103 22-23 0.7814657878345628 1103 24-25 0.7747292285536034 1103 26-27 0.8640975347766116 1103 28-29 0.7505144422737331 1103 30-31 1.1130715192491465 1103 32-33 1.01905524489802 1103 34-35 1.1380509711373392 1103 36-37 1.0045232006308211 1103 38-39 0.8121539924552934 1103 40-41 1.078166842383851 1103 42-43 0.9119123744380815 1103 44-45 1.0242609881773177 1103 46-47 1.0150029375836809 1103 48-49 1.1338694552845539 1103 50-51 1.0963056342689796 1103 52-53 1.188433609867296 1103 54-55 1.297579830415799 1103 56-57 1.2785665593680804 1103 58-59 1.0907134527293252 1103 60-61 1.2772233794871042 1103 62-63 1.3243835946109535 1103 64-65 1.2553638146790966 1103 66-67 1.3645994877660215 1103 68-69 1.3228462037248185 1103 70-71 1.419912028202507 1103 72-73 1.4968664232234836 1103 74-75 1.4623886448582262 1103 76-77 1.2897978139641637 1103 78-79 1.2650321662022606 1103 80-81 1.170860175861609 1103 82-83 1.1658811787027439 1103 84-85 1.366057147599573 1103 86-87 1.4247126561539183 1103 88-89 1.1215703666080117 1103 90-91 1.2095136501624353 1103 92-93 1.2878448147805663 1103 94-95 1.3186402466320608 1103 96-97 1.47598019772175 1103 98-99 1.5352264640950182 1103 100-101 1.3270876327590404 1104 1 0.5406278353258145 1104 2 0.5240643675123522 1104 3 0.476783876260896 1104 4 0.5844280989009576 1104 5 0.6133354485514886 1104 6 0.6015846557633608 1104 7 0.503448722780746 1104 8 0.44981414243100915 1104 9 0.4848071394399014 1104 10-11 0.48762046218858757 1104 12-13 0.5574798717280274 1104 14-15 0.7157372248007192 1104 16-17 0.6599954907592718 1104 18-19 0.6840870523850668 1104 20-21 0.8324160802724165 1104 22-23 0.8220804490611897 1104 24-25 0.7322579043342756 1104 26-27 0.7144451569680825 1104 28-29 0.7695520575684682 1104 30-31 0.7576130382437185 1104 32-33 0.7086807217534314 1104 34-35 0.702291211427351 1104 36-37 0.7586290144226311 1104 38-39 0.8690648394831086 1104 40-41 0.7386099875900669 1104 42-43 0.7044606999308698 1104 44-45 0.8195306008275338 1104 46-47 0.8539052240035474 1104 48-49 0.7078986407238759 1104 50-51 0.440116550448316 1104 52-53 0.6345521701897994 1104 54-55 0.7627731830305251 1104 56-57 0.7827939177709098 1104 58-59 0.7903873080230994 1104 60-61 0.8141066441753608 1104 62-63 0.9841066800761489 1104 64-65 1.0587719670076403 1104 66-67 1.0800725311049675 1104 68-69 0.8964374372276716 1104 70-71 0.8657442220551133 1104 72-73 0.5425741029092315 1104 74-75 0.5058747975200966 1104 76-77 0.7651122876253531 1104 78-79 0.7626564064333401 1104 80-81 0.8690119611401634 1104 82-83 0.7435948002625459 1104 84-85 0.9953921236267398 1104 86-87 0.9422860962623503 1104 88-89 0.7625192985347411 1104 90-91 0.6488395241556653 1104 92-93 0.8161054331295681 1104 94-95 0.6672453841017756 1104 96-97 0.6332662907162678 1104 98-99 0.8214972866665882 1104 100-101 0.6970573432895009 1105 1 0.5592130766269463 1105 2 0.5680289349697425 1105 3 0.5084464901498791 1105 4 0.4759941708161861 1105 5 0.5181541364353421 1105 6 0.41314707348077206 1105 7 0.4813289674275367 1105 8 0.48938576679440615 1105 9 0.5715205508509698 1105 10-11 0.6085006231665417 1105 12-13 0.614202618472568 1105 14-15 0.7836993748657122 1105 16-17 0.650852529712715 1105 18-19 0.6772583800205751 1105 20-21 0.8308039729312284 1105 22-23 0.8286541620709471 1105 24-25 0.7790866469718054 1105 26-27 0.8302342561709821 1105 28-29 0.7706336034961794 1105 30-31 0.7975828032704584 1105 32-33 0.8217357606923841 1105 34-35 0.9978203970265653 1105 36-37 1.0069353793808133 1105 38-39 0.8615159783125463 1105 40-41 1.053020439649913 1105 42-43 0.8955990725874514 1105 44-45 0.9103890232813541 1105 46-47 0.6806908673103678 1105 48-49 0.5728227776886641 1105 50-51 0.6035289167895499 1105 52-53 0.494540724469239 1105 54-55 0.7130475443309905 1105 56-57 0.73329589782346 1105 58-59 0.7593702178406119 1105 60-61 0.9984056937106018 1105 62-63 0.8047629775173171 1105 64-65 0.8523719381956987 1105 66-67 1.0891890299618225 1105 68-69 0.9168487892215715 1105 70-71 0.9943235626173461 1105 72-73 0.95090248967972 1105 74-75 0.8472290291755975 1105 76-77 0.9637479146925045 1105 78-79 1.0602120906763908 1105 80-81 1.0731292143341271 1105 82-83 1.1339529445888665 1105 84-85 1.109717786877919 1105 86-87 1.036214242523375 1105 88-89 0.9105057455015491 1105 90-91 1.0699773675684554 1105 92-93 1.1257731141435734 1105 94-95 1.2517068741492494 1105 96-97 1.25983022259798 1105 98-99 1.4427184914670264 1105 100-101 1.097411717028292 1106 1 0.3382584643048574 1106 2 0.3287436957437677 1106 3 0.11996982141552692 1106 4 0.3892211697876107 1106 5 0.35767340608131803 1106 6 -0.01055400500831638 1106 7 0.164761009708414 1106 8 0.2731091288777918 1106 9 0.15817436362399917 1106 10-11 0.11526603829067028 1106 12-13 0.2307219789913617 1106 14-15 0.4583104984882951 1106 16-17 0.1800523353239143 1106 18-19 -0.106061651640033 1106 20-21 0.1885169017620143 1106 22-23 0.36401290555015464 1106 24-25 0.38666590057675165 1106 26-27 0.26366004271469734 1106 28-29 0.2194399429943772 1106 30-31 0.26899968164443777 1106 32-33 0.372475386375406 1106 34-35 0.2913761316937453 1106 36-37 0.3176137251795197 1106 38-39 0.16867827057268414 1106 40-41 0.30744291657940437 1106 42-43 -0.25399568734368927 1106 44-45 0.012004583867835095 1106 46-47 -0.04648754499671526 1106 48-49 0.3922700207523704 1106 50-51 -0.0070033740087183105 1106 52-53 0.27418633366609413 1106 54-55 -0.024997741938129536 1106 56-57 -0.5050743575291463 1106 58-59 -0.7969067229853763 1106 60-61 -0.0895493123936788 1106 62-63 0.30086960425262177 1106 64-65 0.4271971397292127 1106 66-67 -0.18793683415303164 1106 68-69 -0.35209885091682125 1106 70-71 -0.3274725451288383 1106 72-73 -0.02300087959447339 1106 74-75 -0.03460799767666245 1106 76-77 0.061018042691472374 1106 78-79 0.10707875655286259 1106 80-81 0.1388427700556214 1106 82-83 0.13301129152019797 1106 84-85 0.03252363645312073 1106 86-87 0.11383444157434397 1106 88-89 -0.01613816345179586 1106 90-91 -0.02998667840528313 1106 92-93 -0.1735546904351004 1106 94-95 -0.47958880096332024 1106 96-97 -0.2891877583661433 1106 98-99 -0.23727421236793944 1106 100-101 -0.36949181160257005 1107 1 0.34354377144676107 1107 2 0.3328388925673984 1107 3 0.2625458090913604 1107 4 0.39988351236535635 1107 5 0.3757822429879809 1107 6 0.2870895208387054 1107 7 0.22869177284924547 1107 8 0.23625771542621976 1107 9 0.2707777026196254 1107 10-11 0.2855716178141705 1107 12-13 0.24073150758937345 1107 14-15 0.3463485074356569 1107 16-17 0.46540362682828373 1107 18-19 0.4891228486786545 1107 20-21 0.37556289492868444 1107 22-23 0.5821408596620046 1107 24-25 0.4311265832152884 1107 26-27 0.3151682658990609 1107 28-29 0.2980035122023068 1107 30-31 0.4866435975510228 1107 32-33 0.5077595104795094 1107 34-35 0.5285009511840428 1107 36-37 0.28334399170164914 1107 38-39 0.24559105196744468 1107 40-41 0.42643583849848454 1107 42-43 0.48252968345936864 1107 44-45 0.4767031214016413 1107 46-47 0.5346850297864947 1107 48-49 0.5106122718761483 1107 50-51 0.5467892694678298 1107 52-53 0.44757241934117786 1107 54-55 0.44938305993004235 1107 56-57 0.5026620024949793 1107 58-59 0.4801589103234818 1107 60-61 0.44069530639167454 1107 62-63 0.46287795238464113 1107 64-65 0.36399591657098895 1107 66-67 0.29143437267840966 1107 68-69 0.3955805089739961 1107 70-71 0.4249599692079933 1107 72-73 0.266200106956191 1107 74-75 0.37089514273149504 1107 76-77 0.4912318853483022 1107 78-79 0.5074781175409875 1107 80-81 0.2982639730936292 1107 82-83 0.29349722858687244 1107 84-85 0.23843833256885816 1107 86-87 0.3894890022493982 1107 88-89 0.27822543293171265 1107 90-91 0.3220873026069597 1107 92-93 0.5822823795402989 1107 94-95 0.20966338626355707 1107 96-97 0.12978915396518076 1107 98-99 -0.07119949324297536 1107 100-101 -0.10584572765060685 1108 1 0.10789052821151657 1108 2 0.22886240980822237 1108 3 0.18146021901075926 1108 4 0.21731958749318636 1108 5 0.2857441798154099 1108 6 0.24283593387784208 1108 7 0.18476026047927974 1108 8 0.15211572855304212 1108 9 0.10750451402528682 1108 10-11 0.09083664732353469 1108 12-13 0.1707655634585521 1108 14-15 0.21962902550381358 1108 16-17 0.17755598135706663 1108 18-19 0.2950507598572756 1108 20-21 0.30726196879864887 1108 22-23 0.243550424490806 1108 24-25 0.30487365406486333 1108 26-27 0.3913816498370224 1108 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-0.9842161333640647 2316 50-51 -1.179185775133078 2316 52-53 -1.2026151684091424 2316 54-55 -1.1783714819737057 2316 56-57 -1.085003865401859 2316 58-59 -1.0597657167706487 2316 60-61 -1.0457209969578827 2316 62-63 -1.045740844393535 2316 64-65 -1.0578749994103909 2316 66-67 -0.9381816899845532 2316 68-69 -0.9745836435030704 2316 70-71 -1.2052756502960662 2316 72-73 -1.110587677143716 2316 74-75 -0.9529644989832917 2316 76-77 -0.8762659241926016 2316 78-79 -0.9015037148440257 2316 80-81 -0.7309626628890165 2316 82-83 -0.48623020440619413 2316 84-85 -0.7684358031234879 2316 86-87 -0.8832022538601372 2316 88-89 -0.7853805377847536 2316 90-91 -0.7122668022606078 2316 92-93 -0.8428734861396912 2316 94-95 -0.8452330023819457 2316 96-97 -0.9534794332678693 2316 98-99 -1.1160725521795172 2316 100-101 -0.8333550436995463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6844.0 3 2337.0 4 2473.0 5 2713.0 6 3263.0 7 4055.0 8 5296.0 9 6802.0 10 8322.0 11 10219.0 12 12798.0 13 15136.0 14 17061.0 15 19922.0 16 22415.0 17 25446.0 18 24502.0 19 23895.0 20 24797.0 21 27281.0 22 29665.0 23 32878.0 24 37457.0 25 41728.0 26 46773.0 27 53501.0 28 61069.0 29 70937.0 30 82061.0 31 95914.0 32 113569.0 33 137408.0 34 168658.0 35 208273.0 36 249024.0 37 247696.0 38 117027.0 39 6430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.639144012691496 18.118022962839735 14.645127465440066 23.597705559028697 2 15.66421739212561 19.425551401588518 40.012147084336505 24.898084121949367 3 19.48261215333038 23.262595666817454 29.205731727044565 28.049060452807602 4 13.171071699809358 16.155027976255056 36.28432128520281 34.389579038732776 5 14.250166631790245 36.92294054732731 33.835311601447756 14.991581219434691 6 33.28330849853801 35.394718230031565 17.05704768862011 14.264925582810317 7 29.655445279879277 31.9902504980808 19.896209355686352 18.458094866353562 8 27.069695094253365 34.273251956690814 19.38884223759616 19.26821071145966 9 26.736509314074947 16.64198346403 19.524445572327433 37.097061649567614 10-11 25.33737987387695 25.963975360432855 27.11359743201429 21.5850473336759 12-13 26.449475307603144 23.246596103931637 27.15733846552082 23.1465901229444 14-15 23.0642520074742 24.790523641863167 25.422760276720663 26.722464073941975 16-17 22.895643928938426 27.818342238790212 26.30768329309171 22.97833053917965 18-19 22.955322416375726 26.355260717307367 28.334546490054297 22.354870376262614 20-21 23.303005623086353 25.621550958569244 28.672073489783923 22.40336992856048 22-23 23.094165353920744 25.495564109425494 28.565706515619155 22.844564021034607 24-25 22.977695658935083 25.722198780457674 28.551164921129903 22.74894063947734 26-27 22.42730344017534 26.135403170143977 28.309845852051872 23.12744753762881 28-29 22.636297504586516 26.57174144253924 28.11839507775283 22.673565975121416 30-31 22.726241239870316 25.992007414732154 28.048399194299357 23.233352151098174 32-33 22.366426300524843 26.009116412254414 28.109325263437285 23.515132023783462 34-35 23.262622948040644 26.205941222269715 27.832663524072572 22.698772305617073 36-37 23.44913079255697 25.58072685725799 27.827448773355123 23.142693576829917 38-39 22.748091233413675 26.03754946692541 27.74728620422077 23.467073095440146 40-41 23.28476459471146 25.825943754137043 27.981975447212182 22.907316203939313 42-43 23.023723692461147 25.85855846913305 27.705926928412506 23.411790909993293 44-45 22.879762194376564 26.21213936707007 27.885493106565917 23.022605331987446 46-47 23.383268729283692 26.41188010856966 27.134572349595864 23.070278812550775 48-49 23.090261807075965 26.435184423074503 27.1181225652581 23.356431204591427 50-51 23.296226165709413 26.60123004136127 27.081050749494423 23.021493043434898 52-53 23.54153554171952 26.265771305181413 27.119164738121288 23.07352841497778 54-55 22.985914388258973 26.526223697505763 27.312835370267173 23.17502654396809 56-57 23.08722424474815 26.588263830337304 27.16686843511202 23.157643489802524 58-59 23.080684596577015 26.104553487133554 27.158149555786874 23.656612360502553 60-61 22.701120556741607 26.485847006930463 27.467595989535436 23.345436446792498 62-63 22.852416349318393 26.426421192571098 27.58429457666079 23.13686788144972 64-65 22.548488712090712 26.486820544984973 27.402528145033678 23.562162597890637 66-67 22.535539930014274 26.46758948161374 27.364995710614632 23.631874877757358 68-69 23.076729417244135 26.10985151628266 27.495196029589263 23.31822303688394 70-71 22.813432942420047 26.199777116719318 27.11365667307361 23.873133267787022 72-73 22.777918868058368 26.310273424893797 27.157307377671298 23.754500329376537 74-75 22.81955497020949 26.722825994852933 27.01901218009398 23.43860685484359 76-77 22.997916637415024 26.15404391668293 27.159412823888317 23.688626622013732 78-79 23.00179081759368 26.433658656320365 27.128030144219156 23.4365203818668 80-81 23.242681242654616 26.461978288523706 26.832615386849902 23.462725081971776 82-83 23.063465858476313 26.52586273674426 26.966938107595183 23.443733297184245 84-85 23.02616338249644 26.529800083706945 26.998902707985007 23.44513382581161 86-87 23.199069278567432 26.517039866240268 26.63086248837748 23.653028366814823 88-89 22.97442309349919 26.60082050646323 26.61539631178858 23.809360088248997 90-91 22.94816208109091 26.57324725400585 26.6814114078193 23.797179257083943 92-93 23.092248376377732 26.08300598606681 26.993711300895185 23.83103433666027 94-95 22.8044322971549 26.010448304717038 27.112372543854214 24.07274685427385 96-97 23.108624416479756 25.617722114215606 27.087833552992073 24.18581991631256 98-99 22.857641588162934 25.780302206787926 26.80928598422993 24.552770220819212 100-101 23.444125088455685 25.180037611841914 26.993064104925406 24.382773194776995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 237.0 1 174.5 2 148.5 3 187.5 4 167.0 5 150.5 6 266.0 7 625.5 8 1080.5 9 1251.5 10 1077.0 11 943.5 12 982.0 13 1083.5 14 1222.0 15 1373.0 16 1532.5 17 1711.0 18 1939.0 19 2240.5 20 2552.5 21 3018.5 22 3782.5 23 4580.0 24 5599.0 25 7094.5 26 9219.0 27 11936.5 28 14169.0 29 16597.5 30 19726.5 31 24094.0 32 30769.0 33 33840.0 34 33980.0 35 36357.5 36 40162.0 37 44453.5 38 48109.5 39 51230.5 40 54932.0 41 59576.0 42 63214.0 43 66275.5 44 68956.0 45 73299.0 46 76611.5 47 78749.0 48 80169.5 49 78694.0 50 78268.0 51 77375.0 52 75738.5 53 75756.0 54 76914.0 55 73769.0 56 67648.5 57 62688.0 58 58676.0 59 52391.5 60 42551.0 61 35453.0 62 30659.0 63 23847.5 64 17652.5 65 13770.5 66 10402.5 67 7898.0 68 6002.0 69 4490.5 70 3477.5 71 2548.5 72 1910.0 73 1540.0 74 1198.0 75 922.0 76 668.0 77 461.5 78 333.5 79 216.0 80 133.0 81 93.0 82 61.0 83 32.0 84 18.0 85 14.0 86 13.0 87 8.5 88 5.0 89 4.5 90 2.5 91 3.0 92 3.0 93 0.5 94 2.0 95 2.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18357462197037727 2 0.04608729960859683 3 0.044877023883581155 4 0.05020223707365012 5 0.05872257817776046 6 0.0647255457738382 7 0.03480752985145076 8 0.03287108869142568 9 0.039358166577509686 10-11 0.04047162024452411 12-13 0.03720387578698179 14-15 0.03265323906092286 16-17 0.029966426951388066 18-19 0.037954246736491504 20-21 0.04993597641414667 22-23 0.04611150512309715 24-25 0.06913094941289524 26-27 0.051799801030670806 28-29 0.043424693013562346 30-31 0.05356680358919369 32-33 0.04923401649363758 34-35 0.04853205657312849 36-37 0.04589365549259432 38-39 0.024326542072815028 40-41 0.022365895398289638 42-43 0.01984852189025704 44-45 0.0044780201825579905 46-47 0.011763880047152342 48-49 0.011182947699144819 50-51 0.009004451394116608 52-53 0.008956040365115981 54-55 0.009875849916127893 56-57 0.007673148096599367 58-59 0.00944015065512225 60-61 0.009609589256624444 62-63 0.00440540363905705 64-65 0.006704927516586828 66-67 0.003921293349050781 68-69 0.008036230814104069 70-71 0.021228236216774905 72-73 0.018371985505737917 74-75 0.0188803013102445 76-77 0.0113281807861467 78-79 0.005228391132067708 80-81 0.007140626777592471 82-83 0.006051378625078365 84-85 0.005107363559566141 86-87 0.007285859864594351 88-89 0.028029985791362986 90-91 0.0352432291124564 92-93 0.020937770042771146 94-95 0.03006324900938932 96-97 0.033863514785938534 98-99 0.019533850201752963 100-101 0.11933318648654537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.026031328563455 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17573394290625 43.23850087690143 2 12.798885086586948 14.341414736636077 3 4.281315388041013 7.195953301735397 4 1.9857627010960923 4.450176132108098 5 1.0112964056663833 2.83294620531642 6 0.6223270566988053 2.0919909105131964 7 0.4027874589883293 1.5796607957222824 8 0.2904170811886309 1.3016733191219354 9 0.20996662403247926 1.058723699039675 >10 1.1110685139486072 11.087977519092254 >50 0.06709126737957129 2.5701563056859373 >100 0.037182934844669176 3.8730520829831847 >500 0.003213027169276955 1.292299886047696 >1k 0.0028656728807064684 2.826547190712781 >5k 8.683857214262025E-5 0.25892703838362424 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5322 0.2576434963413365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3983 0.19282112850949704 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2810 0.13603499149176165 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2810 0.13603499149176165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2794 0.13526041502775163 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2613 0.12649801877863814 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2517 0.12185055999457796 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2486 0.12034981809555853 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2212 0.10708519614938675 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2203 0.10664949688838112 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2113 0.10229250427832469 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.841102900062692E-5 2 0.0 0.0 0.0 0.0 4.841102900062692E-5 3 0.0 0.0 0.0 0.0 4.841102900062692E-5 4 0.0 0.0 0.0 0.0 4.841102900062692E-5 5 0.0 0.0 0.0 0.0 4.841102900062692E-5 6 0.0 0.0 0.0 0.0 9.682205800125384E-5 7 0.0 0.0 0.0 0.0 9.682205800125384E-5 8 0.0 0.0 0.0 0.0 9.682205800125384E-5 9 0.0 0.0 0.0 0.0 9.682205800125384E-5 10-11 0.0 0.0 0.0 0.0 9.682205800125384E-5 12-13 0.0 0.0 0.0 0.0 3.3887720300438843E-4 14-15 0.0 0.0 0.0 0.0 3.8728823200501536E-4 16-17 0.0 0.0 0.0 0.0 5.809323480075231E-4 18-19 0.0 0.0 0.0 0.0 6.777544060087769E-4 20-21 0.0 0.0 0.0 2.420551450031346E-5 7.987819785103442E-4 22-23 0.0 0.0 0.0 1.210275725015673E-4 8.713985220112846E-4 24-25 0.0 0.0 0.0 4.841102900062692E-4 8.713985220112846E-4 26-27 0.0 0.0 0.0 0.0010892481525141056 0.0010166316090131655 28-29 0.0 0.0 0.0 0.0034855940880451387 0.0010166316090131655 30-31 0.0 0.0 0.0 0.01195752416315485 0.0010166316090131655 32-33 0.0 0.0 0.0 0.030063249009389316 0.0010166316090131655 34-35 0.0 0.0 0.0 0.05581791643772284 0.0010650426380137924 36-37 0.0 0.0 0.0 0.09536972713123504 0.0010650426380137924 38-39 0.0 0.0 0.0 0.16084564385458294 0.0011134536670144193 40-41 0.0 0.0 0.0 0.2331959266960199 0.0011134536670144193 42-43 0.0 0.0 0.0 0.3087655429659985 0.0011618646960150462 44-45 0.0 0.0 0.0 0.40176312967620287 0.0012344812395159864 46-47 0.0 0.0 0.0 0.5149965265086692 0.0013313032975172403 48-49 0.0 0.0 0.0 0.6368954975322478 0.0013555088120175537 50-51 0.0 0.0 0.0 0.7715991857264922 0.0014523308700188076 52-53 0.0 0.0 0.0 0.8985086982516357 0.0015975639570206884 54-55 0.0 0.0 0.0 1.0371820908239315 0.0015975639570206884 56-57 0.0 0.0 0.0 1.2019732335420654 0.0016217694715210018 58-59 0.0 0.0 0.0 1.3763739655168241 0.0016701805005216288 60-61 0.0 0.0 0.0 1.5716882620198533 0.0017427970440225691 62-63 0.0 0.0 0.0 1.761314262615309 0.001839619102023823 64-65 0.0 0.0 0.0 1.9592911657133727 0.00188803013102445 66-67 0.0 0.0 0.0 2.1931648468154017 0.001936441160025077 68-69 0.0 0.0 0.0 2.448799285453212 0.001984852189025704 70-71 0.0 0.0 0.0 2.720845062922235 0.0020090577035260177 72-73 0.0 0.0 0.0 3.000442960915356 0.0021300852760275848 74-75 0.0 0.0 0.0 3.290546052201613 0.002251112848529152 76-77 0.0 0.0 0.0 3.6026035451396536 0.0023237293920300925 78-79 0.0 0.0 0.0 3.9573595656562475 0.0023237293920300925 80-81 0.0 0.0 0.0 4.325936934952521 0.0023479349065304057 82-83 0.0 0.0 0.0 4.7178242147125955 0.0024689624790319733 84-85 4.841102900062692E-5 0.0 0.0 5.121112291802318 0.002493167993532287 86-87 4.841102900062692E-5 0.0 0.0 5.523335326254027 0.002614195566033854 88-89 4.841102900062692E-5 0.0 0.0 5.950223779981555 0.0027110176240351075 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3230 0.0 50.8619 1 GTATCAA 5915 0.0 43.553047 1 TATCAAC 7715 0.0 33.204483 2 ATCAACG 7685 0.0 33.086727 3 TCAACGC 7815 0.0 32.47552 4 CAACGCA 7965 0.0 31.862387 5 AACGCAG 8345 0.0 30.463276 6 ACGCAGA 9345 0.0 26.994154 7 CGCAGAG 9545 0.0 26.378761 8 GTACATG 7150 0.0 24.042366 1 TAAGGTG 1345 0.0 24.027548 5 TACATGG 7035 0.0 23.915697 2 GCAGAGT 10425 0.0 23.513517 9 ACATGGG 7315 0.0 22.350538 3 AGAGTAC 9290 0.0 21.834885 10-11 AGGTAAG 1370 0.0 21.508743 2 AAGGTAA 1295 0.0 21.327219 1 GAGTACT 5940 0.0 20.591475 12-13 GTAAGGT 1505 0.0 20.526775 4 AGTACTT 6295 0.0 20.26027 12-13 >>END_MODULE