FastQCFastQC Report
Thu 26 May 2016
SRR938618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065645
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49480.239537771495102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.19906615125057792No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29270.141699081884835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.13821348779678985No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27620.13371126209973155No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26640.12896698125767012No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26190.12678848495264192No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24990.12097916147256668No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23400.113281807861467No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21570.10442258955435228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.050.851861
GTATCAA55800.041.4160351
ATCAACG75250.029.8134693
TATCAAC76250.029.8094862
TCAACGC75900.029.5574324
CAACGCA78000.028.7616565
AACGCAG80700.027.8975646
TAAGGTG14600.027.6714925
ACGCAGA93450.024.0913167
CGCAGAG94900.023.6231238
GTAAGGT14950.023.2085574
AGGTAAG14500.022.9560132
TACATGG68100.022.2047772
TATACCG1302.9301317E-421.9368555
GTACATG69400.021.9258751
AAGGTAA14100.021.9210151
GGTAAGG15350.021.6753773
ACATGGG68950.021.0942543
AGAGTAC91100.021.0110710-11
GCAGAGT106750.020.5558599
Produced by FastQC (version 0.11.5)