##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065645 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77604815929165 31.0 31.0 34.0 30.0 34.0 2 32.00718903780659 31.0 31.0 34.0 30.0 34.0 3 32.0705014656439 33.0 31.0 34.0 30.0 34.0 4 35.74458341099269 37.0 35.0 37.0 33.0 37.0 5 35.61024425784682 37.0 35.0 37.0 33.0 37.0 6 35.639820491904466 37.0 35.0 37.0 33.0 37.0 7 35.577696554829124 37.0 35.0 37.0 33.0 37.0 8 35.558839490812794 37.0 35.0 37.0 33.0 37.0 9 37.13969244473276 39.0 37.0 39.0 33.0 39.0 10-11 37.04681636970535 39.0 37.0 39.0 33.0 39.0 12-13 36.94067615684205 39.0 37.0 39.0 33.0 39.0 14-15 37.99496985203169 40.0 37.0 41.0 33.0 41.0 16-17 37.91714210331398 40.0 37.0 41.0 33.0 41.0 18-19 38.02285557295663 40.0 37.0 41.0 33.0 41.0 20-21 38.06357796233138 40.0 37.0 41.0 33.0 41.0 22-23 37.97355741184957 40.0 37.0 41.0 33.0 41.0 24-25 37.87093571257404 40.0 37.0 41.0 32.0 41.0 26-27 37.67823900040907 40.0 37.0 41.0 32.0 41.0 28-29 37.55660483771413 39.5 36.5 41.0 32.0 41.0 30-31 37.31497014249787 39.0 36.0 41.0 31.0 41.0 32-33 37.09761938764889 39.0 36.0 41.0 31.0 41.0 34-35 36.951092515896974 39.0 36.0 41.0 30.5 41.0 36-37 36.945166037726715 39.0 36.0 41.0 30.5 41.0 38-39 36.813817960007654 39.0 35.0 41.0 30.0 41.0 40-41 36.636804242742585 39.0 35.0 41.0 30.0 41.0 42-43 36.417062951281565 39.0 35.0 40.0 30.0 41.0 44-45 36.15464709570134 39.0 35.0 40.0 29.5 41.0 46-47 35.80662698575989 38.0 35.0 40.0 28.5 41.0 48-49 35.454306282057175 38.0 34.5 40.0 27.5 41.0 50-51 35.08859145690572 38.0 34.0 40.0 26.5 41.0 52-53 34.58527990046693 38.0 33.0 40.0 25.5 41.0 54-55 34.25857831331133 37.5 33.0 40.0 24.0 41.0 56-57 33.91188272912335 37.0 33.0 40.0 24.0 41.0 58-59 33.45690304965277 36.5 32.0 39.5 23.0 41.0 60-61 33.08311374897429 36.0 31.5 39.0 22.0 40.5 62-63 32.6197616240932 35.5 31.0 39.0 21.0 40.0 64-65 32.10827271869077 35.0 31.0 38.5 19.5 40.0 66-67 31.61036601158476 35.0 30.0 38.0 18.0 40.0 68-69 31.064453959901144 34.5 29.5 37.5 15.0 39.5 70-71 30.489074356919993 34.0 29.0 37.0 12.0 39.0 72-73 30.046949500035097 34.0 29.0 36.0 8.0 39.0 74-75 29.524138949335438 34.0 28.0 36.0 7.0 38.5 76-77 28.284683718644782 31.5 26.5 34.5 7.0 36.5 78-79 28.97760263743286 33.0 28.0 35.0 4.0 37.0 80-81 28.71616105381128 33.0 28.0 35.0 2.0 37.0 82-83 28.2451885488552 33.0 27.0 35.0 2.0 36.0 84-85 27.730740519305108 33.0 26.0 35.0 2.0 36.0 86-87 27.16450551764703 32.5 25.5 35.0 2.0 35.0 88-89 26.520834170440708 32.0 25.0 34.0 2.0 35.0 90-91 25.98996536190875 32.0 24.0 34.0 2.0 35.0 92-93 25.254752631744566 31.0 20.5 34.0 2.0 35.0 94-95 24.478953063086834 31.0 18.0 34.0 2.0 35.0 96-97 23.480287513101235 31.0 4.5 34.0 2.0 35.0 98-99 22.235550881201753 30.0 2.0 34.0 2.0 35.0 100-101 18.59168564782429 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35452148825959995 1101 2 0.3584194612632601 1101 3 0.26058061068985694 1101 4 0.19909446083051563 1101 5 0.16780014736610127 1101 6 0.24416915460593458 1101 7 0.36489297803671406 1101 8 0.3119380706103456 1101 9 0.28420836141954453 1101 10-11 0.42515305669264336 1101 12-13 0.4998530846517397 1101 14-15 0.522569408488657 1101 16-17 0.49689087489234396 1101 18-19 0.5627839959475978 1101 20-21 0.5541507959636007 1101 22-23 0.4802182708689813 1101 24-25 0.5310940333273919 1101 26-27 0.600213187230537 1101 28-29 0.6731609203675291 1101 30-31 0.44694878291549145 1101 32-33 0.5182838271719916 1101 34-35 0.3473437976592848 1101 36-37 0.45229474999996455 1101 38-39 0.490620264255881 1101 40-41 0.5765255600822172 1101 42-43 0.6798601620169791 1101 44-45 0.5671953324503605 1101 46-47 0.6629760337469293 1101 48-49 0.7592076718885536 1101 50-51 0.7081366433597438 1101 52-53 0.7463600492037941 1101 54-55 0.8525590719463949 1101 56-57 0.8326685437559078 1101 58-59 0.9217287839712256 1101 60-61 0.9233300923861734 1101 62-63 1.0326528372540125 1101 64-65 0.9739401525967182 1101 66-67 0.8824818445665166 1101 68-69 0.7673163855657634 1101 70-71 0.6480952058561122 1101 72-73 0.5767381202607957 1101 74-75 0.6182777978580525 1101 76-77 0.7835966342843328 1101 78-79 0.7961299452878521 1101 80-81 0.8378152820926559 1101 82-83 0.8067263564701008 1101 84-85 0.8315006862917187 1101 86-87 0.8806499172888174 1101 88-89 0.8021588222377538 1101 90-91 0.7362237575019037 1101 92-93 0.6395908974078672 1101 94-95 0.7268039188302957 1101 96-97 0.6972904540509788 1101 98-99 0.7080912815854106 1101 100-101 0.7551558314523064 1102 1 0.35499277381593686 1102 2 0.3326725149121543 1102 3 0.3747122852533522 1102 4 0.29008994600225435 1102 5 0.27636230415518526 1102 6 0.23037713568849938 1102 7 0.26365103624315367 1102 8 0.329488725313837 1102 9 0.38869542703356075 1102 10-11 0.3879651802345023 1102 12-13 0.4092589930485886 1102 14-15 0.5828417833892772 1102 16-17 0.4827998735828416 1102 18-19 0.4764936005627973 1102 20-21 0.5267592037755762 1102 22-23 0.4840358780197107 1102 24-25 0.4390072138884875 1102 26-27 0.49382253567218726 1102 28-29 0.34245800111509794 1102 30-31 0.3376258227180031 1102 32-33 0.39233749451135935 1102 34-35 0.27266760626784503 1102 36-37 0.22424010611420186 1102 38-39 0.3970336792463556 1102 40-41 0.5427628763852042 1102 42-43 0.5071366751057127 1102 44-45 0.46267890249426813 1102 46-47 0.4069638667167226 1102 48-49 0.3863666790728715 1102 50-51 0.45031577846580717 1102 52-53 0.24435696424911413 1102 54-55 0.3509513135474691 1102 56-57 0.39815466679880984 1102 58-59 0.3488008629957662 1102 60-61 0.4653012017564535 1102 62-63 0.33517278316537613 1102 64-65 0.22834553195003338 1102 66-67 0.38238889793002073 1102 68-69 0.5318769736680125 1102 70-71 0.3866770877172989 1102 72-73 0.4594613062482331 1102 74-75 0.2965870621381015 1102 76-77 0.31970555318147476 1102 78-79 0.18373464979520548 1102 80-81 0.22215326421390102 1102 82-83 0.019304535997378025 1102 84-85 0.020812114311222984 1102 86-87 -0.018670674346861205 1102 88-89 0.1428846893594944 1102 90-91 0.0400922790242646 1102 92-93 0.16666797565097724 1102 94-95 0.3166391484691573 1102 96-97 0.3452027706694487 1102 98-99 0.22277369530521085 1102 100-101 0.24560392645881635 1103 1 0.22791697908309771 1103 2 0.246398055547715 1103 3 0.22965817882403172 1103 4 0.23495455130828446 1103 5 0.14462901198047007 1103 6 0.07238093142743907 1103 7 0.16080308874325056 1103 8 0.12998948846315272 1103 9 0.30872195147434667 1103 10-11 0.28679287426592737 1103 12-13 0.3884753215857941 1103 14-15 0.38211279000324083 1103 16-17 0.44658100693103364 1103 18-19 0.36442712368307895 1103 20-21 0.31720754685550645 1103 22-23 0.4955967327469537 1103 24-25 0.45929569576440343 1103 26-27 0.42779511374493495 1103 28-29 0.3878525235894159 1103 30-31 0.3198983213317632 1103 32-33 0.4732656212799 1103 34-35 0.5848571302570278 1103 36-37 0.47229364661257023 1103 38-39 0.4534131216614483 1103 40-41 0.5916787470328231 1103 42-43 0.504895102617958 1103 44-45 0.5337075248811232 1103 46-47 0.5244917202377906 1103 48-49 0.4511143253145704 1103 50-51 0.49576675112390944 1103 52-53 0.755044627525912 1103 54-55 0.7577734968976628 1103 56-57 0.6631146800610352 1103 58-59 0.7075474974215155 1103 60-61 0.6374378197759185 1103 62-63 0.5941069179484089 1103 64-65 0.67230621975623 1103 66-67 0.6972322951163683 1103 68-69 0.605417271953641 1103 70-71 0.46550298105931986 1103 72-73 0.3729424676071531 1103 74-75 0.5315055737968528 1103 76-77 0.6183827610267656 1103 78-79 0.6550250867053364 1103 80-81 0.6750702381410605 1103 82-83 0.5078187099954228 1103 84-85 0.6058303784466368 1103 86-87 0.734018926814727 1103 88-89 0.8868438051352499 1103 90-91 1.052914330895554 1103 92-93 0.838393722079612 1103 94-95 0.8949555610385396 1103 96-97 0.8751335155832152 1103 98-99 0.8543590737072257 1103 100-101 0.24677008721750582 1104 1 0.2898119608560066 1104 2 0.2858689773807157 1104 3 0.2767574061870377 1104 4 0.19390147774893762 1104 5 0.25892514437570213 1104 6 0.11576660260691085 1104 7 0.15373738743544862 1104 8 0.24513643313549238 1104 9 0.3718086689503437 1104 10-11 0.340571415380694 1104 12-13 0.3273416050186242 1104 14-15 0.5097738803504868 1104 16-17 0.4591973605742865 1104 18-19 0.46419717877409283 1104 20-21 0.4143753272207036 1104 22-23 0.4165523358154033 1104 24-25 0.40116825163579506 1104 26-27 0.454760407233735 1104 28-29 0.48401986289501053 1104 30-31 0.44018800725864793 1104 32-33 0.3712054401231839 1104 34-35 0.43047866980368354 1104 36-37 0.5271385805503073 1104 38-39 0.456082153844072 1104 40-41 0.46774721163894384 1104 42-43 0.5528319058657729 1104 44-45 0.4608525800872414 1104 46-47 0.47343907024298915 1104 48-49 0.5555694877838064 1104 50-51 0.6029959161216425 1104 52-53 0.5960422923961133 1104 54-55 0.4748087798249756 1104 56-57 0.38690086835757853 1104 58-59 0.41024903357965314 1104 60-61 0.36149299458377726 1104 62-63 0.329711593180285 1104 64-65 0.5530597917405586 1104 66-67 0.4717578337569712 1104 68-69 0.2935470364793744 1104 70-71 -0.19722292387253404 1104 72-73 -0.20607188846883773 1104 74-75 -0.03928541459103485 1104 76-77 -0.01580225933656365 1104 78-79 -0.010309883797074093 1104 80-81 0.09629747499927888 1104 82-83 0.1208324086255601 1104 84-85 0.22475487438179442 1104 86-87 0.15251129482602366 1104 88-89 -0.08389916392335905 1104 90-91 -0.04551861837522608 1104 92-93 -0.05179624662265425 1104 94-95 -0.09891944229933358 1104 96-97 -0.3579801857172207 1104 98-99 -0.21621725492730448 1104 100-101 -0.46763805958628524 1105 1 0.22468510814587717 1105 2 0.3388948134816019 1105 3 0.199262279731812 1105 4 0.23085609037212151 1105 5 0.14291622233550783 1105 6 0.22612623145109012 1105 7 0.30071761238382777 1105 8 0.2657892388852332 1105 9 0.3229829643039608 1105 10-11 0.3372457440041927 1105 12-13 0.37760318541086946 1105 14-15 0.49500907651216863 1105 16-17 0.3667517557463569 1105 18-19 0.4024316829659895 1105 20-21 0.3892650150261616 1105 22-23 0.3931015652605936 1105 24-25 0.5144648541321999 1105 26-27 0.40140286044781703 1105 28-29 0.5111550267091403 1105 30-31 0.4283535889755328 1105 32-33 0.5039734927717419 1105 34-35 0.4877888551993266 1105 36-37 0.5046225511612477 1105 38-39 0.43519536084844646 1105 40-41 0.41622587729455773 1105 42-43 0.44600470484294874 1105 44-45 0.40916324926880066 1105 46-47 0.14065759983682824 1105 48-49 0.2948222702116823 1105 50-51 0.2759291806752415 1105 52-53 0.41557685651773113 1105 54-55 0.37430538333155283 1105 56-57 0.2819129542215606 1105 58-59 0.4355473261425473 1105 60-61 0.255248181337663 1105 62-63 0.30603507050087586 1105 64-65 0.39902349527883274 1105 66-67 0.26268940604805024 1105 68-69 0.2687817209655208 1105 70-71 0.06712238288792705 1105 72-73 0.2532123543183502 1105 74-75 0.2035716059157373 1105 76-77 0.3121502457543919 1105 78-79 0.4487460814283146 1105 80-81 0.41148312647533203 1105 82-83 0.3161636658299152 1105 84-85 0.4324838321646105 1105 86-87 0.5182115096199489 1105 88-89 0.5716721591444021 1105 90-91 0.728465074327822 1105 92-93 0.6916198837180154 1105 94-95 0.599341322047291 1105 96-97 0.5230563503316397 1105 98-99 0.06923159322079186 1105 100-101 -0.18134780164691122 1106 1 0.1571106352411178 1106 2 0.15400287969495707 1106 3 0.14864414704960183 1106 4 0.10234020145821177 1106 5 0.01772180060926587 1106 6 0.11068944271194425 1106 7 0.09248585246716345 1106 8 0.11019259301395579 1106 9 0.010854604479227703 1106 10-11 0.13028488648092207 1106 12-13 0.16904316391747187 1106 14-15 0.3119490859682088 1106 16-17 -0.021380188945023804 1106 18-19 0.17997569372322175 1106 20-21 0.10631538885584035 1106 22-23 0.26244687273337064 1106 24-25 0.21029313348839196 1106 26-27 0.1364763423121076 1106 28-29 0.3078946023462734 1106 30-31 0.30078824286206185 1106 32-33 0.21753777475139913 1106 34-35 0.130719938047676 1106 36-37 -0.33427246176508163 1106 38-39 -0.05561627824688031 1106 40-41 0.24720310246026855 1106 42-43 0.2686114244265667 1106 44-45 0.15106832537149018 1106 46-47 0.0415275424025765 1106 48-49 0.12047718004345853 1106 50-51 -0.007340146589370988 1106 52-53 -0.03200138299930444 1106 54-55 -0.3211027738863166 1106 56-57 -0.030138361261187185 1106 58-59 -0.07340068275508571 1106 60-61 -0.37974502954126166 1106 62-63 -0.4594475497442758 1106 64-65 -0.816230494235235 1106 66-67 -0.6319152511177997 1106 68-69 -1.1872654696816909 1106 70-71 -0.9631771154678006 1106 72-73 -0.752502755459961 1106 74-75 -0.4424906380106002 1106 76-77 -0.33556943927253613 1106 78-79 -0.4243599118312922 1106 80-81 -1.027786506332653 1106 82-83 -0.3219735468506606 1106 84-85 -0.23792738707197714 1106 86-87 -0.41067787972791336 1106 88-89 -0.1662516341238991 1106 90-91 -0.39322679308216024 1106 92-93 -0.5745620620928129 1106 94-95 -0.2617539850962345 1106 96-97 -0.281088701116861 1106 98-99 -0.6000977127348008 1106 100-101 -1.0989590846261734 1107 1 0.17665669163173092 1107 2 0.1441776649585904 1107 3 0.13850324124555868 1107 4 0.11051366394841011 1107 5 0.059330654875722644 1107 6 0.09180177258060951 1107 7 -0.029124433446497733 1107 8 0.10498636937780503 1107 9 0.16206698248380746 1107 10-11 0.10486071813292597 1107 12-13 0.09913259471768754 1107 14-15 0.19275588346342687 1107 16-17 0.129084110531565 1107 18-19 0.21482130085951212 1107 20-21 0.3081557841962237 1107 22-23 0.20139033424198516 1107 24-25 0.1562653826790239 1107 26-27 0.19020940526128527 1107 28-29 0.2661969130848689 1107 30-31 0.2738746684890643 1107 32-33 0.20200075917001215 1107 34-35 0.25006402073687184 1107 36-37 0.13889594066665722 1107 38-39 0.16495822805986649 1107 40-41 0.10861493806773126 1107 42-43 0.2088953406417744 1107 44-45 0.2526898801489281 1107 46-47 0.261082750190738 1107 48-49 0.14286242001796978 1107 50-51 -0.028185722863803164 1107 52-53 0.07044111857701552 1107 54-55 0.22460556357264494 1107 56-57 0.18292374779517928 1107 58-59 0.08349981390647798 1107 60-61 -0.0788263963957192 1107 62-63 0.07630280270240775 1107 64-65 -0.0029942300114598197 1107 66-67 0.06737968406587314 1107 68-69 0.15258635911902374 1107 70-71 -9.106636339417662E-4 1107 72-73 -0.1217730054452808 1107 74-75 -0.08968261632843522 1107 76-77 -0.12790043953529207 1107 78-79 0.01243886501048408 1107 80-81 0.30253940343842345 1107 82-83 0.046742904275948405 1107 84-85 0.15205920516617866 1107 86-87 0.1848391356562935 1107 88-89 -0.030943077717214607 1107 90-91 0.07052831466215181 1107 92-93 -0.3710190138758769 1107 94-95 -0.44276252054257625 1107 96-97 -0.17521571137028502 1107 98-99 -0.4718040133112744 1107 100-101 -0.8239077232111214 1108 1 0.17368474627429364 1108 2 0.17678461245525767 1108 3 0.11344910849659584 1108 4 0.06602390275347148 1108 5 0.08654947661675294 1108 6 0.080536199518626 1108 7 0.12248472862346205 1108 8 0.051046120010099116 1108 9 0.0015150757960213923 1108 10-11 0.048778445048029084 1108 12-13 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-0.7665596551037837 2316 56-57 -0.5054432781419678 2316 58-59 -0.6498501633971969 2316 60-61 -0.564363032483925 2316 62-63 -0.517027539642605 2316 64-65 -0.5216728817442942 2316 66-67 -0.36620802402773833 2316 68-69 -0.42152705915837885 2316 70-71 -0.3630844435746141 2316 72-73 -0.5568807338072368 2316 74-75 -0.2277160590967533 2316 76-77 -0.2638521544272443 2316 78-79 -0.15640879515694195 2316 80-81 -0.3703191720769894 2316 82-83 -0.2768324527952757 2316 84-85 -0.3402283730833524 2316 86-87 -0.12285808483584759 2316 88-89 0.15217360249686962 2316 90-91 -0.003525670917767343 2316 92-93 0.15090426199529006 2316 94-95 0.28891426270226006 2316 96-97 0.4428353606351223 2316 98-99 0.728228922748432 2316 100-101 0.9839657896518865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 33.0 5 62.0 6 135.0 7 386.0 8 934.0 9 1716.0 10 2701.0 11 4212.0 12 5976.0 13 8062.0 14 10690.0 15 13800.0 16 17051.0 17 20751.0 18 19961.0 19 17757.0 20 17673.0 21 18723.0 22 20509.0 23 22975.0 24 26640.0 25 31269.0 26 37393.0 27 45222.0 28 53794.0 29 64125.0 30 76739.0 31 91484.0 32 110322.0 33 135283.0 34 172831.0 35 225439.0 36 296008.0 37 329934.0 38 158331.0 39 6709.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.14232842167841 19.01094629195631 15.356518177738424 23.490207108626855 2 16.07091136466889 20.225731863133046 38.92622618133137 24.77713059086669 3 19.405691059272105 23.515147466191845 29.735392563803902 27.343768910732148 4 13.625235701197447 16.910989061528 35.90195798406793 33.56181725320663 5 14.125923051169535 37.07684756798032 34.02811996261524 14.769109418234901 6 33.24162670739648 35.84318699486117 16.73070638952966 14.184479908212689 7 29.475055007031703 32.124251747033014 19.880715224542456 18.519978021392834 8 27.005705239767725 34.70504370305643 19.18756611131148 19.101684945864367 9 26.375442053208566 16.568771497522565 19.50364171965657 37.5521447296123 10-11 25.220960039116115 26.201622253581814 27.11438799987413 21.463029707427946 12-13 26.298589544670065 23.312984564143402 27.249115893582875 23.139309997603654 14-15 22.78368257856505 24.905174896945024 25.465556763141777 26.84558576134815 16-17 22.713075092767635 28.020569846222365 26.338843315284088 22.927511745725912 18-19 22.797649160431728 26.52612138097301 28.402750714667818 22.27347874392744 20-21 23.097507074061614 25.704731451919375 28.752762454342353 22.44499901967666 22-23 22.97463988245802 25.59474159402995 28.65027630594802 22.780342217564005 24-25 22.956751629096768 25.855855223457542 28.583510619113657 22.60388252833204 26-27 22.420762394908653 26.29676610351664 28.360054515569132 22.922416986005572 28-29 22.57050461236079 26.606386867056052 28.204410728852245 22.618697791730913 30-31 22.663599021128995 26.138349038677994 28.111413142142045 23.08663879805097 32-33 22.305236378951854 26.100830491202505 28.1543295193511 23.43960361049454 34-35 23.21195699405767 26.259280427783878 27.87794632173215 22.65081625642631 36-37 23.37934162841383 25.651443957859104 27.961258533666534 23.007955880060532 38-39 22.67819635833383 26.101375143998094 27.868397298463787 23.352031199204283 40-41 23.20537331381948 25.851653392501046 28.04091034745861 22.90206294622087 42-43 22.987924813653486 25.893439373056598 27.76089843298037 23.35773738030955 44-45 22.844365803417336 26.191092854774173 28.038820804155602 22.92572053765289 46-47 23.351664976314904 26.473208126275328 27.19458086941367 22.9805460279961 48-49 23.056164055295078 26.43384995969782 27.201818318249266 23.30816766675784 50-51 23.246128933093537 26.593751588486892 27.147283294080054 23.012836184339516 52-53 23.579729333936857 26.229361773199173 27.210992208244882 22.979916684619088 54-55 23.017411026580074 26.506514914227758 27.41080873044497 23.065265328747195 56-57 23.082790121245424 26.590048144768343 27.192329756565144 23.134831977421097 58-59 23.027553137155707 26.21675070014451 27.140529955534472 23.615166207165316 60-61 22.681535307373725 26.56538272549252 27.432182199748745 23.320899767385008 62-63 22.7722333702064 26.599076801676958 27.488726281621478 23.139963546495164 64-65 22.464871747081418 26.592226641073367 27.31045266732667 23.63244894451854 66-67 22.4658429520113 26.601698987119022 27.25113372715753 23.68132433371215 68-69 23.01095481962926 26.207669801882776 27.41062552390432 23.370749854583647 70-71 22.7866335083071 26.303117237586555 26.99902645326419 23.911222800842157 72-73 22.834151076298205 26.41540535764858 26.9340811223613 23.816362443691922 74-75 22.83158529176117 26.811359163844706 26.759051047009528 23.598004497384593 76-77 22.900159514340558 26.278184295946303 27.12068143364421 23.700974756068927 78-79 22.890114225822927 26.545558409116765 27.061523156205446 23.50280420885486 80-81 23.162552196886498 26.62241514821362 26.72930817506541 23.48572447983447 82-83 23.017276561012107 26.629586794543652 26.889216902728514 23.463919741715728 84-85 22.93151361400519 26.669870056934812 26.833242960610406 23.565373368449592 86-87 23.15807513638352 26.657873378096962 26.475770487906487 23.70828099761303 88-89 22.89391595488179 26.82293942242128 26.33546350393498 23.947681118761956 90-91 22.867818586329708 26.727849236516953 26.53107513120493 23.873257045948414 92-93 22.90030104711725 26.34299038522751 26.7389241069343 24.01778446072094 94-95 22.652341183834768 26.219535515091426 26.8397056025654 24.288417698508407 96-97 22.87500998477473 25.83713561623609 26.910892239469998 24.37696215951918 98-99 22.557401603906964 26.113629965674136 26.605171705572623 24.723796724846274 100-101 22.787240789196595 25.876203316639597 26.794270070607485 24.542285823556323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 174.5 2 136.5 3 178.5 4 173.5 5 171.0 6 284.5 7 630.0 8 1004.0 9 1163.0 10 1047.0 11 894.0 12 924.5 13 1003.0 14 1162.0 15 1341.0 16 1485.0 17 1686.0 18 1948.5 19 2183.0 20 2467.5 21 2946.5 22 3538.0 23 4324.0 24 5446.5 25 6947.5 26 9078.0 27 11671.5 28 14025.5 29 16667.0 30 19968.0 31 24633.0 32 31650.0 33 34475.0 34 34149.5 35 36722.0 36 40600.5 37 44522.5 38 48224.0 39 51508.5 40 54875.5 41 59859.5 42 63544.0 43 66425.0 44 69514.5 45 73932.0 46 77042.0 47 79112.5 48 80388.5 49 79042.0 50 78776.5 51 77780.5 52 76009.0 53 77025.0 54 79266.5 55 75542.0 56 68733.5 57 62954.0 58 58745.5 59 51985.0 60 41479.0 61 34655.0 62 30068.0 63 22717.0 64 15998.5 65 12321.0 66 9150.5 67 6934.5 68 5248.5 69 3882.5 70 3022.5 71 2111.5 72 1547.5 73 1326.5 74 1047.0 75 777.5 76 533.5 77 336.5 78 237.5 79 171.5 80 106.5 81 57.0 82 34.0 83 23.5 84 11.5 85 7.0 86 4.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.777544060087769E-4 2 0.047588041507616266 3 9.198095510119115E-4 4 0.0 5 0.08239557135906701 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.1467168850407497E-4 26-27 0.008713985220112847 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01014211057563134 36-37 1.4523308700188078E-4 38-39 0.0038486768055498405 40-41 0.0050347470160651995 42-43 1.9364411600250768E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.210275725015673E-4 68-69 0.0011860702105153595 70-71 9.682205800125384E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013797143265178674 82-83 6.777544060087769E-4 84-85 0.006051378625078365 86-87 0.0026384010805341673 88-89 2.1784963050282117E-4 90-91 0.003800265776549213 92-93 0.001040837123513479 94-95 9.682205800125384E-5 96-97 0.0 98-99 7.261654350094039E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.880805157744724 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47391870004594 40.66595507968642 2 13.609766151695416 14.666103165239397 3 4.667610846162863 7.544838916628318 4 2.102075743946206 4.530461343455473 5 1.1312291873156781 3.0475769715254986 6 0.6996459538782627 2.2618492392191474 7 0.4319065618495696 1.6290031313757656 8 0.3267862856977785 1.4086006550324084 9 0.2358985392507981 1.1439362907331962 >10 1.1973128941828761 11.586904698849656 >50 0.07552176932832828 2.773708836523714 >100 0.04176008561454604 4.241441949357924 >500 0.00350854740045748 1.3589309143440482 >1k 0.003058733631168052 3.1406888080290267 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4948 0.239537771495102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4112 0.19906615125057792 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2927 0.141699081884835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.13821348779678985 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2762 0.13371126209973155 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2664 0.12896698125767012 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2619 0.12678848495264192 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2499 0.12097916147256668 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2340 0.113281807861467 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2157 0.10442258955435228 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.682205800125384E-5 2 0.0 0.0 0.0 0.0 9.682205800125384E-5 3 0.0 0.0 0.0 0.0 9.682205800125384E-5 4 0.0 0.0 0.0 0.0 9.682205800125384E-5 5 0.0 0.0 0.0 0.0 9.682205800125384E-5 6 0.0 0.0 0.0 0.0 9.682205800125384E-5 7 0.0 0.0 0.0 0.0 9.682205800125384E-5 8 0.0 0.0 0.0 0.0 9.682205800125384E-5 9 0.0 0.0 0.0 0.0 9.682205800125384E-5 10-11 0.0 0.0 0.0 0.0 9.682205800125384E-5 12-13 0.0 0.0 0.0 0.0 3.6308271750470195E-4 14-15 0.0 0.0 0.0 0.0 4.5990477550595575E-4 16-17 0.0 0.0 0.0 0.0 5.325213190068962E-4 18-19 0.0 0.0 0.0 0.0 6.2934337700815E-4 20-21 0.0 0.0 0.0 2.420551450031346E-5 7.019599205090904E-4 22-23 0.0 0.0 0.0 1.210275725015673E-4 8.229874930106576E-4 24-25 0.0 0.0 0.0 4.841102900062692E-4 8.229874930106576E-4 26-27 0.0 0.0 0.0 0.0011376591815147326 8.713985220112846E-4 28-29 0.0 0.0 0.0 0.0034855940880451387 8.95604036511598E-4 30-31 0.0 0.0 0.0 0.012005935192155478 9.44015065512225E-4 32-33 0.0 0.0 0.0 0.030039043494889006 0.0010650426380137924 34-35 0.0 0.0 0.0 0.05605997158272597 0.0011134536670144193 36-37 0.0 0.0 0.0 0.09566019330523881 0.0011134536670144193 38-39 0.0 0.0 0.0 0.16162022031859297 0.0012102757250156732 40-41 0.0 0.0 0.0 0.23438199690653527 0.0012586867540163 42-43 0.0 0.0 0.0 0.3103873124375195 0.0012828922685166133 44-45 0.0 0.0 0.0 0.40382059840872947 0.0013555088120175537 46-47 0.0 0.0 0.0 0.517683338618204 0.0014039198410181807 48-49 0.0 0.0 0.0 0.6394128710402804 0.0015007418990194345 50-51 0.0 0.0 0.0 0.774213381292526 0.0016943860150219422 52-53 0.0 0.0 0.0 0.9015101820496746 0.0017427970440225691 54-55 0.0 0.0 0.0 1.040231985650971 0.0018638246165241365 56-57 0.0 0.0 0.0 1.2053135945431088 0.0019122356455247634 58-59 0.0 0.0 0.0 1.3809003967283826 0.0019364411600250769 60-61 0.0 0.0 0.0 1.5769408586664215 0.001984852189025704 62-63 0.0 0.0 0.0 1.7669783530083825 0.001984852189025704 64-65 0.0 0.0 0.0 1.966092915287961 0.0020090577035260177 66-67 0.0 0.0 0.0 2.199409869556482 0.002081674247026958 68-69 0.0 0.0 0.0 2.4556010350278 0.0021300852760275848 70-71 0.0 0.0 0.0 2.7297042812293495 0.0021300852760275848 72-73 0.0 0.0 0.0 3.011722730672502 0.0022027018195285254 74-75 0.0 0.0 0.0 3.3063764586848174 0.0022753183630294656 76-77 0.0 0.0 0.0 3.622839355261916 0.0023479349065304057 78-79 2.420551450031346E-5 0.0 0.0 3.983404699258585 0.0025173735080326 80-81 4.841102900062692E-5 4.841102900062692E-5 0.0 4.35907428430345 0.002565784537033227 82-83 4.841102900062692E-5 4.841102900062692E-5 0.0 4.7583442459861205 0.0027594286530357344 84-85 4.841102900062692E-5 4.841102900062692E-5 0.0 5.169402293230444 0.0027594286530357344 86-87 4.841102900062692E-5 4.841102900062692E-5 0.0 5.581113889366275 0.002832045196536675 88-89 4.841102900062692E-5 4.841102900062692E-5 0.0 6.020540799604966 0.0028562507110369883 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3170 0.0 50.85186 1 GTATCAA 5580 0.0 41.416035 1 ATCAACG 7525 0.0 29.813469 3 TATCAAC 7625 0.0 29.809486 2 TCAACGC 7590 0.0 29.557432 4 CAACGCA 7800 0.0 28.761656 5 AACGCAG 8070 0.0 27.897564 6 TAAGGTG 1460 0.0 27.671492 5 ACGCAGA 9345 0.0 24.091316 7 CGCAGAG 9490 0.0 23.623123 8 GTAAGGT 1495 0.0 23.208557 4 AGGTAAG 1450 0.0 22.956013 2 TACATGG 6810 0.0 22.204777 2 TATACCG 130 2.9301317E-4 21.936855 5 GTACATG 6940 0.0 21.925875 1 AAGGTAA 1410 0.0 21.921015 1 GGTAAGG 1535 0.0 21.675377 3 ACATGGG 6895 0.0 21.094254 3 AGAGTAC 9110 0.0 21.01107 10-11 GCAGAGT 10675 0.0 20.555859 9 >>END_MODULE