FastQCFastQC Report
Thu 26 May 2016
SRR938617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067193
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54430.2633039101815844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40290.1949019757710093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.13694899315158285No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28060.135739623731311No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27980.135352625516824No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26110.12630654225319068No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25430.12301705743005129No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24230.11721208421274645No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23120.1118424839867395No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22090.10685988197521953No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21430.10366714670570187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.056.656241
GTATCAA59050.040.354551
TATCAAC76150.030.7694022
ATCAACG76200.030.6244663
TCAACGC77200.030.2893414
CAACGCA78700.029.7120345
AACGCAG82200.028.4985356
ACGCAGA91850.025.393567
CGCAGAG94100.024.6349378
GTACATG72150.023.7137641
TACATGG70450.023.07212
TGGTATC15250.022.439062
GAGTACT59900.022.04487812-13
ACATGGG70550.021.961533
GCAGAGT104200.021.6083419
GTGGTAT17750.021.4799861
GTACTTT62150.021.09700214-15
CAGAGTA98400.020.75743310-11
GTACCGT1656.308631E-520.1586536
GTGTAAG9250.020.0939461
Produced by FastQC (version 0.11.5)