FastQCFastQC Report
Thu 26 May 2016
SRR938617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067193
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50620.2448731202166416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.20099719764917934No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30070.14546295387029656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.14386658623553777No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27610.1335627587748217No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26960.13041839828211493No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26900.1301281496212497No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24820.120066196044588No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23860.11542221747074416No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA20850.10086140965067122No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA20760.10042603665937336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34100.050.765811
GTATCAA61150.042.8528251
ATCAACG79800.031.9894243
TCAACGC81500.031.322164
TATCAAC82350.031.1278782
CAACGCA82750.030.7915695
AACGCAG85650.029.8325636
AAGGTAA12150.027.0082911
ACGCAGA98700.025.7918997
TAAGGTG15050.025.5848885
CGCAGAG100000.025.409118
AGGTAAG13400.024.4888632
GTAAGGT15950.024.4392684
TAGCGCG1601.8480732E-623.7686774
TAGCCCT13400.023.0591644
GGTAAGG15650.022.4777263
GCAGAGT110200.022.45399
TACATGG68500.022.355772
GTACATG71150.021.9241751
TGGTATC16800.021.7974532
Produced by FastQC (version 0.11.5)