##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2067193 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784764170544307 31.0 31.0 34.0 30.0 34.0 2 32.01897984368175 31.0 31.0 34.0 30.0 34.0 3 32.087248263708325 33.0 31.0 34.0 30.0 34.0 4 35.76454351383736 37.0 35.0 37.0 35.0 37.0 5 35.63068760391507 37.0 35.0 37.0 33.0 37.0 6 35.67101862283783 37.0 35.0 37.0 33.0 37.0 7 35.60311398113287 37.0 35.0 37.0 33.0 37.0 8 35.581357909009945 37.0 35.0 37.0 33.0 37.0 9 37.14846799500579 39.0 37.0 39.0 33.0 39.0 10-11 37.07935543512386 39.0 37.0 39.0 33.0 39.0 12-13 36.971616099706225 39.0 37.0 39.0 33.0 39.0 14-15 38.02343104877 40.0 37.0 41.0 33.0 41.0 16-17 37.945961262446225 40.0 37.0 41.0 33.0 41.0 18-19 37.93766571384481 40.0 37.0 41.0 33.0 41.0 20-21 37.91952299567578 40.0 37.0 41.0 32.5 41.0 22-23 37.952729377469836 40.0 37.0 41.0 33.0 41.0 24-25 37.87911747959673 40.0 37.0 41.0 32.0 41.0 26-27 37.699937548163135 40.0 37.0 41.0 32.0 41.0 28-29 37.56647952078011 39.5 36.5 41.0 32.0 41.0 30-31 37.3158691520337 39.0 36.0 41.0 31.0 41.0 32-33 37.098054221352335 39.0 36.0 41.0 31.0 41.0 34-35 36.93443161814112 39.0 36.0 40.5 30.5 41.0 36-37 36.937742629739944 39.0 36.0 41.0 30.5 41.0 38-39 36.78307637458138 39.0 35.0 41.0 30.0 41.0 40-41 36.603784213665584 39.0 35.0 40.5 30.0 41.0 42-43 36.37020007323942 39.0 35.0 40.0 30.0 41.0 44-45 36.091941826428396 38.5 35.0 40.0 29.5 41.0 46-47 35.74190653702871 38.0 35.0 40.0 28.5 41.0 48-49 35.349628457526705 38.0 34.0 40.0 27.0 41.0 50-51 34.97181806439941 38.0 34.0 40.0 26.0 41.0 52-53 34.44786698677869 38.0 33.0 40.0 24.5 41.0 54-55 34.11134035380344 37.0 33.0 40.0 24.0 41.0 56-57 33.735126328310905 37.0 32.5 40.0 23.0 41.0 58-59 33.269030758134335 36.5 32.0 39.0 22.5 41.0 60-61 32.84242013203412 36.0 31.0 39.0 21.5 40.0 62-63 32.354735624588514 35.5 31.0 39.0 19.5 40.0 64-65 31.841553739781432 35.0 30.5 38.0 18.0 40.0 66-67 31.342313223777364 35.0 30.0 38.0 15.5 40.0 68-69 30.76965600212462 34.0 29.0 37.5 12.0 39.5 70-71 30.197386504308014 34.0 29.0 37.0 8.0 39.0 72-73 29.60907012552771 34.0 28.0 36.0 7.0 39.0 74-75 28.98560390829497 33.5 27.0 35.5 4.0 38.5 76-77 27.732687513937982 31.5 26.0 34.5 2.0 36.0 78-79 28.352607134408835 33.0 27.0 35.0 2.0 37.0 80-81 28.056585669552867 33.0 26.0 35.0 2.0 36.5 82-83 27.56238048406704 33.0 26.0 35.0 2.0 36.0 84-85 26.968574535614238 32.0 25.0 35.0 2.0 36.0 86-87 26.335925092625605 32.0 24.5 34.5 2.0 35.0 88-89 25.58158551233484 31.5 21.5 34.0 2.0 35.0 90-91 25.045914193788388 31.0 19.5 34.0 2.0 35.0 92-93 24.26690686355846 31.0 16.5 34.0 2.0 35.0 94-95 23.47398985000433 31.0 4.5 34.0 2.0 35.0 96-97 22.488076585011655 30.0 2.0 34.0 2.0 35.0 98-99 21.311659337081736 29.5 2.0 34.0 2.0 35.0 100-101 18.176425229768096 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33809480498331723 1101 2 0.320246833036272 1101 3 0.35374729367093494 1101 4 0.28365357372987177 1101 5 0.16656915022254282 1101 6 0.18071897633296885 1101 7 0.368557952853628 1101 8 0.29673842252230287 1101 9 0.3961003005384143 1101 10-11 0.37611040504460647 1101 12-13 0.4190611527079895 1101 14-15 0.5469936904591322 1101 16-17 0.544557511313748 1101 18-19 0.5608246919407307 1101 20-21 0.5858009425365225 1101 22-23 0.5510119523623587 1101 24-25 0.5343666648638958 1101 26-27 0.6461776789650742 1101 28-29 0.5099388652208958 1101 30-31 0.6800117795383045 1101 32-33 0.547775590335867 1101 34-35 0.49704046501113197 1101 36-37 0.6920875706879954 1101 38-39 0.864019031877362 1101 40-41 0.8474890613993793 1101 42-43 0.6346528033607655 1101 44-45 0.7064209501741701 1101 46-47 0.8301381371037522 1101 48-49 0.8679868290659769 1101 50-51 0.8880531010738721 1101 52-53 0.9611377763200011 1101 54-55 0.9157510324305704 1101 56-57 1.0221439140590647 1101 58-59 1.2093199901784928 1101 60-61 1.2678646002834455 1101 62-63 1.5177887494968019 1101 64-65 1.4245470348329015 1101 66-67 1.5245682753887984 1101 68-69 1.595634982872486 1101 70-71 1.6472826804984741 1101 72-73 1.7357912026767046 1101 74-75 1.5180770290486834 1101 76-77 1.4525256330997713 1101 78-79 1.7185778377823908 1101 80-81 1.9220758680380676 1101 82-83 2.169866431235331 1101 84-85 2.411764211310601 1101 86-87 2.4073153823722713 1101 88-89 2.7539592109689366 1101 90-91 2.95060860533151 1101 92-93 3.132813543190469 1101 94-95 3.263038971756302 1101 96-97 3.800175180815394 1101 98-99 4.0132404437454525 1101 100-101 4.610936074228295 1102 1 0.3030101901815492 1102 2 0.27641202613597926 1102 3 0.30496364851843083 1102 4 0.19381725474393363 1102 5 0.21533474345725523 1102 6 0.16299056791118005 1102 7 0.32389418288636307 1102 8 0.35937335170059015 1102 9 0.36521038137062334 1102 10-11 0.41002295157877455 1102 12-13 0.4163600157459513 1102 14-15 0.5821246226815475 1102 16-17 0.545740149454268 1102 18-19 0.6089888712827403 1102 20-21 0.6072530340530946 1102 22-23 0.49718624030327874 1102 24-25 0.48381701959595347 1102 26-27 0.44885739115100165 1102 28-29 0.4283801818802715 1102 30-31 0.41590200867652527 1102 32-33 0.46767186781664805 1102 34-35 0.5604762105161498 1102 36-37 0.6226322231755148 1102 38-39 0.6583487819841878 1102 40-41 0.7081313438838563 1102 42-43 0.6116681990338648 1102 44-45 0.7191160801230367 1102 46-47 0.6707997968968371 1102 48-49 0.7684666062863172 1102 50-51 0.8285776137967886 1102 52-53 1.033209701811188 1102 54-55 0.8856755872904714 1102 56-57 1.0892229403671152 1102 58-59 1.1598452653574114 1102 60-61 1.3002831130952046 1102 62-63 1.3569829783937024 1102 64-65 1.41244536168141 1102 66-67 1.3442097833034943 1102 68-69 1.4360474786950057 1102 70-71 1.6878643417927144 1102 72-73 1.7516583633641858 1102 74-75 1.7422134407407235 1102 76-77 1.7063000872133394 1102 78-79 2.1319788689744463 1102 80-81 2.288710912178569 1102 82-83 2.3208589660547467 1102 84-85 2.4275729407904976 1102 86-87 2.5300116548175176 1102 88-89 2.501139964584251 1102 90-91 2.6980576436987214 1102 92-93 3.063460331528649 1102 94-95 3.139371102341027 1102 96-97 3.4750787871372673 1102 98-99 3.781424247422297 1102 100-101 4.241697159709112 1103 1 0.3473723625976852 1103 2 0.2980143315739099 1103 3 0.2892862794518649 1103 4 0.24635965287991723 1103 5 0.20401336321178576 1103 6 0.24739501041445777 1103 7 0.26653249193388007 1103 8 0.2483806146309675 1103 9 0.25863905444004587 1103 10-11 0.4373230813038944 1103 12-13 0.446101571273978 1103 14-15 0.5262939302585892 1103 16-17 0.5304734679494558 1103 18-19 0.5119999049023107 1103 20-21 0.5863502245217944 1103 22-23 0.546542550410571 1103 24-25 0.3901630271261567 1103 26-27 0.4260983390290676 1103 28-29 0.545385743390753 1103 30-31 0.5916965401062271 1103 32-33 0.6538936493438996 1103 34-35 0.6741052404460461 1103 36-37 0.8129569224469861 1103 38-39 0.9267824605992914 1103 40-41 0.956422473870667 1103 42-43 1.0505493222131221 1103 44-45 0.9850822575344793 1103 46-47 1.1241151676936951 1103 48-49 1.0940908560364946 1103 50-51 1.1087469688683171 1103 52-53 1.2558372826938182 1103 54-55 1.3456240045602073 1103 56-57 1.2382778467797095 1103 58-59 1.4954842151147716 1103 60-61 1.6039106669711742 1103 62-63 1.7305185652625 1103 64-65 1.6596675166456443 1103 66-67 1.6250683903742136 1103 68-69 1.7063023610809829 1103 70-71 1.854872743659346 1103 72-73 1.7825532897430882 1103 74-75 2.1191968009099895 1103 76-77 1.9079334782540194 1103 78-79 2.3307247586065856 1103 80-81 2.4296584643037633 1103 82-83 2.472782981405711 1103 84-85 2.600093263305954 1103 86-87 2.6114783076393877 1103 88-89 3.0315962654720217 1103 90-91 3.232591368970162 1103 92-93 3.4917747477795835 1103 94-95 3.590101981854982 1103 96-97 3.9283692466137694 1103 98-99 4.340491199738771 1103 100-101 4.444415008041542 1104 1 0.3344127989919947 1104 2 0.32809836449699503 1104 3 0.23006844468253718 1104 4 0.21675142009977577 1104 5 0.16842214331042982 1104 6 0.2006376625541222 1104 7 0.2200300451573156 1104 8 0.2671068303288564 1104 9 0.2266942664255538 1104 10-11 0.34121795617187445 1104 12-13 0.4114713079203156 1104 14-15 0.5505704630196533 1104 16-17 0.5286725016529914 1104 18-19 0.5313617758990858 1104 20-21 0.572201616961749 1104 22-23 0.6033740820169342 1104 24-25 0.5689940512123783 1104 26-27 0.7222442209503086 1104 28-29 0.7318967751080194 1104 30-31 0.7509494722673935 1104 32-33 0.6725652107807676 1104 34-35 0.6666917721756462 1104 36-37 0.688578315151652 1104 38-39 0.7502644869921582 1104 40-41 0.7837134116236868 1104 42-43 0.8455070729860452 1104 44-45 0.8198313910533486 1104 46-47 0.8489677038796017 1104 48-49 1.1450635421416777 1104 50-51 1.0855442383003648 1104 52-53 1.1726601152587222 1104 54-55 1.244189575524942 1104 56-57 1.2894844394299767 1104 58-59 1.4641682363539346 1104 60-61 1.4814810146498587 1104 62-63 1.516161472805294 1104 64-65 1.7198897803498348 1104 66-67 1.720343432523027 1104 68-69 1.7320331079480091 1104 70-71 1.794639982685819 1104 72-73 1.887890877361059 1104 74-75 1.977084882675502 1104 76-77 1.9372753998750163 1104 78-79 2.259147187893788 1104 80-81 2.4948280225878 1104 82-83 2.648056686293696 1104 84-85 2.700294605495941 1104 86-87 2.824864174481071 1104 88-89 2.9744338416799145 1104 90-91 3.1599389691574693 1104 92-93 3.418256779649294 1104 94-95 3.6199341944146433 1104 96-97 4.042773394221804 1104 98-99 4.291396497938809 1104 100-101 4.452789318758892 1105 1 0.25360558814998413 1105 2 0.2658023880761604 1105 3 0.2638127737537843 1105 4 0.1674126196485375 1105 5 0.1902599082441867 1105 6 0.20480770140434146 1105 7 0.18767461915674488 1105 8 0.05829234578126119 1105 9 0.21229335934332028 1105 10-11 0.21328537734935793 1105 12-13 0.3158963748571395 1105 14-15 0.4980823797946883 1105 16-17 0.4925366044686754 1105 18-19 0.38306046850555475 1105 20-21 0.34150219760625333 1105 22-23 0.2083091829293906 1105 24-25 0.1319379752220371 1105 26-27 0.3036039297534714 1105 28-29 0.40094694476417914 1105 30-31 0.4125513523142956 1105 32-33 0.3482914159655053 1105 34-35 0.413760580702359 1105 36-37 0.5084646457438851 1105 38-39 0.520796700797554 1105 40-41 0.6264528248171288 1105 42-43 0.636325773299518 1105 44-45 0.6373043998964292 1105 46-47 0.7307531180222142 1105 48-49 0.8373410457299215 1105 50-51 1.0128505787749518 1105 52-53 0.9657487409039547 1105 54-55 0.9383831748948879 1105 56-57 0.9623790093228592 1105 58-59 0.988241520358315 1105 60-61 1.2355456149578714 1105 62-63 1.333354459077853 1105 64-65 1.3871492705955468 1105 66-67 1.38377472677114 1105 68-69 1.2084961176656677 1105 70-71 1.408999735440208 1105 72-73 1.3814981940411677 1105 74-75 1.545862554743433 1105 76-77 1.3471010289435554 1105 78-79 1.758726099652602 1105 80-81 2.0239206251528614 1105 82-83 2.0285121319613246 1105 84-85 1.98635159025552 1105 86-87 2.2224346262952146 1105 88-89 2.3222334169956653 1105 90-91 2.5157187992748895 1105 92-93 2.5727654205043073 1105 94-95 2.746590633912678 1105 96-97 3.255426396645337 1105 98-99 3.6016201075595973 1105 100-101 4.2252445095982125 1106 1 0.19182465540844262 1106 2 0.19174252109609569 1106 3 0.13054808936239937 1106 4 0.07622374398114573 1106 5 -4.6118154737939676E-4 1106 6 0.11377947138113598 1106 7 0.15670649997458952 1106 8 0.041109936879799136 1106 9 0.11310335159139129 1106 10-11 0.17500788135659207 1106 12-13 0.1581236621885651 1106 14-15 0.03161170674728453 1106 16-17 0.0960211019415027 1106 18-19 0.22878324485389356 1106 20-21 0.4118309471245425 1106 22-23 0.29272668894358844 1106 24-25 0.14741046476672182 1106 26-27 0.11068497614846251 1106 28-29 0.23402521790805508 1106 30-31 0.21727411175255895 1106 32-33 0.2571732955818362 1106 34-35 0.16599293215409006 1106 36-37 0.3681707131652203 1106 38-39 0.2700132751560105 1106 40-41 0.29886275868096845 1106 42-43 0.5502034654490657 1106 44-45 0.0316006785245051 1106 46-47 -0.1971114252145938 1106 48-49 0.4107474275895058 1106 50-51 0.2262209243900415 1106 52-53 0.3933972357744153 1106 54-55 0.45558449637218956 1106 56-57 0.5597324947103388 1106 58-59 0.9255211888497783 1106 60-61 0.5738106064004853 1106 62-63 0.7713134227932983 1106 64-65 0.3441992558659912 1106 66-67 0.38277925777931543 1106 68-69 0.29850818611405927 1106 70-71 0.6453533107180398 1106 72-73 1.0698042591484906 1106 74-75 1.2655626057406373 1106 76-77 1.3707497587749629 1106 78-79 1.5742557274264115 1106 80-81 1.5324172667243943 1106 82-83 1.7718408877096365 1106 84-85 1.9188183419471976 1106 86-87 2.0707978207316238 1106 88-89 2.3580255780222323 1106 90-91 2.3702755285473778 1106 92-93 2.1198092291499826 1106 94-95 2.640047861676159 1106 96-97 3.019020088656042 1106 98-99 3.5715237468731935 1106 100-101 4.094416654978513 1107 1 0.10485683270258406 1107 2 0.1622034915615913 1107 3 0.17859250349336975 1107 4 0.04170240425782623 1107 5 0.036678578098964465 1107 6 0.08533880202853794 1107 7 0.08177943997763748 1107 8 0.08526004725507619 1107 9 0.16711454485662358 1107 10-11 0.23927899254248075 1107 12-13 0.21541041486899815 1107 14-15 0.30445391000776567 1107 16-17 0.3044822932869735 1107 18-19 0.26585106751660703 1107 20-21 0.21098375163153094 1107 22-23 0.20332260758210907 1107 24-25 0.2969329412453803 1107 26-27 0.38593615162250927 1107 28-29 0.2785483867936591 1107 30-31 0.2857911227460832 1107 32-33 0.3309555113789884 1107 34-35 0.3236752411781154 1107 36-37 0.31204239397984423 1107 38-39 0.3815074030705361 1107 40-41 0.5201563431099672 1107 42-43 0.5852804563563154 1107 44-45 0.5755231142784112 1107 46-47 0.43459785791340977 1107 48-49 0.5639851742513926 1107 50-51 0.6093565442625035 1107 52-53 0.6631086163993629 1107 54-55 0.8821489154556943 1107 56-57 0.7650863750580967 1107 58-59 0.7891832407643449 1107 60-61 0.821684205247081 1107 62-63 0.9470825445495024 1107 64-65 1.0001928253251897 1107 66-67 1.1880761493682321 1107 68-69 1.3394571546434086 1107 70-71 1.3572765368081114 1107 72-73 1.5440877042375973 1107 74-75 1.3817085866957797 1107 76-77 1.4430670269728623 1107 78-79 1.7566556703082306 1107 80-81 1.9372073207407645 1107 82-83 2.0493512295947376 1107 84-85 2.3187643514614287 1107 86-87 2.478819862137126 1107 88-89 2.6864213595598585 1107 90-91 2.7499790964060615 1107 92-93 2.8074442862530624 1107 94-95 2.8560489548594674 1107 96-97 3.210933185328564 1107 98-99 3.4214935052986597 1107 100-101 3.6092768728990414 1108 1 0.16507328847093916 1108 2 0.24037836616259511 1108 3 0.18163129168306114 1108 4 0.12431611834811207 1108 5 0.11080292260421487 1108 6 0.04395000813888572 1108 7 0.20342715429592317 1108 8 0.1603213217648829 1108 9 0.1516462936026315 1108 10-11 0.14231920572077428 1108 12-13 0.21412614389316076 1108 14-15 0.3351285708604834 1108 16-17 0.3363129761415138 1108 18-19 0.30444154004282353 1108 20-21 0.4693840594290606 1108 22-23 0.38539518225151426 1108 24-25 0.32432300337487874 1108 26-27 0.33912527610950605 1108 28-29 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7 -0.40479298457368884 2316 8 -0.42850430700007536 2316 9 -0.6713708586334377 2316 10-11 -0.5143743043283635 2316 12-13 -0.4523795651330147 2316 14-15 -0.8323931990851037 2316 16-17 -0.682276058988819 2316 18-19 -0.5965287384944915 2316 20-21 -0.5372839894563697 2316 22-23 -0.6752951015602235 2316 24-25 -0.8649370483628971 2316 26-27 -0.7565193467703182 2316 28-29 -0.6751348435800395 2316 30-31 -0.6164408061658264 2316 32-33 -0.6955329487284345 2316 34-35 -0.6320340418876 2316 36-37 -0.48862791420180685 2316 38-39 -0.5288766173023234 2316 40-41 -0.4616853613824503 2316 42-43 -0.4591177500436885 2316 44-45 -0.2596872498149807 2316 46-47 -0.24824796388821113 2316 48-49 -0.24710257395081925 2316 50-51 -0.1884057381036044 2316 52-53 -0.06353620375968205 2316 54-55 -0.1477405802088967 2316 56-57 -0.010893097143878094 2316 58-59 0.055720855513918366 2316 60-61 0.4137603917145185 2316 62-63 0.563353544537236 2316 64-65 0.43720927067545645 2316 66-67 0.6051947774970721 2316 68-69 0.608625840368962 2316 70-71 0.7151213418174827 2316 72-73 0.6276266017593599 2316 74-75 0.8357446278383129 2316 76-77 0.8648355697664734 2316 78-79 1.001006555520025 2316 80-81 1.1512697403792096 2316 82-83 1.2190807542502817 2316 84-85 1.5270997022396848 2316 86-87 1.4959354345093843 2316 88-89 1.6495164278054482 2316 90-91 1.767772853064976 2316 92-93 1.9722917017823711 2316 94-95 2.593978554962071 2316 96-97 2.784160841130724 2316 98-99 3.0736050868958635 2316 100-101 3.5771404277725765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 30.0 5 62.0 6 165.0 7 369.0 8 918.0 9 1677.0 10 2720.0 11 4247.0 12 5934.0 13 8201.0 14 11043.0 15 14354.0 16 17663.0 17 22034.0 18 21409.0 19 19396.0 20 19721.0 21 21297.0 22 22875.0 23 26314.0 24 29718.0 25 34679.0 26 41268.0 27 49001.0 28 58381.0 29 68953.0 30 81347.0 31 96370.0 32 114579.0 33 139672.0 34 173735.0 35 217767.0 36 275418.0 37 300328.0 38 156957.0 39 8569.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.19337149137574 18.941201397838228 15.303658295891237 23.561768814894794 2 16.054757050192833 20.222998599906788 38.92312360650073 24.799120743399648 3 19.421745869267816 23.492106184826923 29.69028457982535 27.395863366079908 4 13.651362016028498 16.82818198397537 35.94163679927322 33.57881920072291 5 14.1229992228111 37.138443733702644 33.93250320196985 14.806053841516414 6 33.28750629476783 35.8308101855995 16.709083283467002 14.17260023616566 7 29.465318429387093 32.094148925620395 19.918991598752513 18.521541046239996 8 27.098969472129596 34.697679413581604 19.15689536487401 19.046455749414786 9 26.413789133380384 16.584711732286245 19.45362624583191 37.54787288850146 10-11 25.228534539348768 26.21400130515148 27.080998242544357 21.476465912955394 12-13 26.334986621955476 23.294414212896424 27.249874588391116 23.120724576756984 14-15 22.846512154404547 24.947162650028325 25.375327799581367 26.83099739598576 16-17 22.73450035869897 28.013784876400027 26.337623047291665 22.914091717609338 18-19 22.803434415654465 26.543820533448013 28.350449135615303 22.302295915282222 20-21 23.10463996346737 25.689304288472336 28.746928806357218 22.459126941703072 22-23 22.981816405144563 25.58537108049418 28.672600961787314 22.76021155257395 24-25 22.978391982284982 25.829992417178538 28.535741453603897 22.655874146932586 26-27 22.45140305696652 26.240293069356746 28.35251053363142 22.955793340045314 28-29 22.582990557727314 26.620446179916435 28.17385701286721 22.622706249489042 30-31 22.699501207676303 26.103440752750227 28.092514825659727 23.104543213913747 32-33 22.360577306820627 26.067768487097187 28.171498922447675 23.400155283634508 34-35 23.20843923012128 26.250090112058277 27.887250825039395 22.65421983278105 36-37 23.383690725933537 25.624025064769924 27.93399720239395 23.058287006902585 38-39 22.651000298509917 26.142840624248585 27.8494029559739 23.356756121267598 40-41 23.254727695068595 25.85686789642333 28.021572788446907 22.866831620061166 42-43 23.00943265698277 25.913610252946917 27.78004077260901 23.2969163174613 44-45 22.885052397336896 26.200796995845703 27.962484353363042 22.951666253454352 46-47 23.347455556840178 26.471501665787052 27.164761262989245 23.016281514383525 48-49 23.057329431746336 26.480497950602583 27.158736508879432 23.303436108771653 50-51 23.271992503844587 26.59858078079792 27.138273978288435 22.991152737069058 52-53 23.608003703572912 26.2545877428958 27.177844545719726 22.959564007811558 54-55 23.03974520037558 26.51208667986008 27.344785900494053 23.103382219270287 56-57 23.108195509562968 26.547859827311722 27.21417400310469 23.12977066002062 58-59 23.076461655975034 26.21625073227318 27.10794299322801 23.599344618523766 60-61 22.687770324299667 26.607675238838368 27.365732178853158 23.338822258008808 62-63 22.807667208625414 26.606103058592012 27.446058495747614 23.140171237034952 64-65 22.45973162641321 26.565951993838986 27.318760270569804 23.655556109178004 66-67 22.534887973420897 26.552253592803364 27.240531582033178 23.67232685174256 68-69 23.063476733276396 26.199423807336576 27.36791795434431 23.369181505042715 70-71 22.83202390874969 26.352982038929117 26.95790862294909 23.8570854293721 72-73 22.87150325864669 26.45169716898644 26.90970966661305 23.767089905753817 74-75 22.864507571378194 26.843236214518917 26.67798797693297 23.614268237169924 76-77 22.900643529655916 26.30373651613565 27.099719281170167 23.69590067303827 78-79 22.946115819858136 26.59652485278346 27.038549375892817 23.418809951465587 80-81 23.158553652223087 26.64746349276531 26.72919267818728 23.46479017682432 82-83 23.01265279634515 26.670380275656857 26.81400648180376 23.502960446194233 84-85 22.97619689764112 26.71159102019274 26.764176653865345 23.548035428300796 86-87 23.247439362487974 26.684593023958946 26.452584002890923 23.61538361066216 88-89 22.900315984194986 26.859237071350893 26.318140743578727 23.92230620087539 90-91 22.905983278100802 26.68673275220053 26.552442845156143 23.85484112454252 92-93 22.952420804137027 26.374925321149295 26.733117742243678 23.939536132470003 94-95 22.70620111426461 26.203552353360333 26.80949480769333 24.28075172468173 96-97 22.922665141892963 25.89807816593718 26.88736701767422 24.291889674495646 98-99 22.636565623045357 26.138343154219275 26.560050271068064 24.665040951667308 100-101 22.8175968525184 25.804182661712726 26.826083791103468 24.552136694665407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 262.0 1 184.5 2 150.5 3 191.0 4 165.5 5 158.5 6 285.5 7 657.5 8 1030.5 9 1179.5 10 1086.5 11 940.0 12 955.0 13 1040.5 14 1213.5 15 1409.0 16 1505.0 17 1700.5 18 1939.5 19 2179.5 20 2550.0 21 3024.0 22 3691.0 23 4485.0 24 5502.5 25 7058.0 26 9171.0 27 11619.0 28 14061.5 29 16891.5 30 19936.0 31 24405.5 32 31634.0 33 34542.5 34 34220.0 35 36613.0 36 40364.5 37 44563.5 38 48483.5 39 51732.5 40 54958.5 41 59615.5 42 63211.5 43 65878.0 44 68865.5 45 74021.5 46 77160.0 47 78594.0 48 79655.5 49 78545.0 50 78480.5 51 77191.5 52 76001.0 53 76950.0 54 79180.0 55 75790.0 56 68766.0 57 63162.5 58 58668.0 59 52196.0 60 42029.0 61 35046.5 62 30462.0 63 23215.5 64 16463.5 65 12657.5 66 9487.0 67 7228.0 68 5386.5 69 3914.0 70 3085.0 71 2249.0 72 1633.5 73 1328.0 74 1030.5 75 774.5 76 554.5 77 383.0 78 267.0 79 180.5 80 118.0 81 69.0 82 40.5 83 23.5 84 12.0 85 7.0 86 3.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.837477681087349E-5 2 0.04363404868340789 3 2.4187388405436745E-4 4 0.0 5 0.09955529067677764 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.916829246229065E-4 26-27 0.011706695988231385 28-29 0.0 30-31 0.0 32-33 3.869982144869879E-4 34-35 0.015576678133101264 36-37 0.003313672211544834 38-39 0.012577441970827106 40-41 0.006990155249171219 42-43 0.005756598440493945 44-45 0.0026848001130034787 46-47 9.674955362174698E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.4187388405436745E-4 68-69 1.2093694202718373E-4 70-71 0.0 72-73 2.4187388405436744E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.00104005770143378 84-85 0.004281167747762304 86-87 0.0015479928579479516 88-89 4.837477681087349E-5 90-91 0.001136807255055527 92-93 3.869982144869879E-4 94-95 0.0 96-97 4.837477681087349E-5 98-99 0.0 100-101 7.981838173794126E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2067193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.68066853120046 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33227218851289 40.438867330537306 2 13.7547081188985 14.76723854548004 3 4.606573811595597 7.418518855343156 4 2.125836832454532 4.5646536941763545 5 1.1372387384239955 3.052386787908954 6 0.6940943792459027 2.2355670181001184 7 0.45388128645056214 1.7055255623327228 8 0.31714621063103443 1.3619696487048671 9 0.23870740606422633 1.1532575826788762 >10 1.2155255818769188 11.764463751306355 >50 0.07598289397419478 2.772893704609113 >100 0.041468568159305594 4.22719026097704 >500 0.0033269506487107925 1.288958307042138 >1k 0.003147115478510202 3.0041745083880875 >5k 8.991758510029149E-5 0.24433444241489258 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5062 0.2448731202166416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.20099719764917934 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3007 0.14546295387029656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2974 0.14386658623553777 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2761 0.1335627587748217 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2696 0.13041839828211493 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2690 0.1301281496212497 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2482 0.120066196044588 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2386 0.11542221747074416 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2085 0.10086140965067122 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2076 0.10042603665937336 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4187388405436745E-4 2 0.0 0.0 0.0 0.0 2.4187388405436745E-4 3 0.0 0.0 0.0 0.0 2.4187388405436745E-4 4 0.0 0.0 0.0 0.0 2.4187388405436745E-4 5 0.0 0.0 0.0 0.0 2.4187388405436745E-4 6 0.0 0.0 0.0 0.0 2.9024866086524095E-4 7 0.0 0.0 0.0 0.0 2.9024866086524095E-4 8 0.0 0.0 0.0 0.0 2.9024866086524095E-4 9 0.0 0.0 0.0 0.0 2.9024866086524095E-4 10-11 0.0 0.0 0.0 0.0 2.9024866086524095E-4 12-13 0.0 0.0 0.0 0.0 5.079351565141716E-4 14-15 0.0 0.0 0.0 0.0 6.046847101359186E-4 16-17 0.0 0.0 0.0 2.4187388405436744E-5 6.772468753522288E-4 18-19 0.0 0.0 0.0 4.837477681087349E-5 6.772468753522288E-4 20-21 0.0 0.0 0.0 4.837477681087349E-5 8.223712057848494E-4 22-23 0.0 0.0 0.0 1.2093694202718373E-4 9.191207594065963E-4 24-25 0.0 0.0 0.0 4.837477681087349E-4 9.191207594065963E-4 26-27 0.0 0.0 0.0 0.0010884324782446534 9.191207594065963E-4 28-29 0.0 0.0 0.0 0.0032894848231393974 9.191207594065963E-4 30-31 0.0 0.0 0.0 0.011609946434609638 9.43308147812033E-4 32-33 0.0 0.0 0.0 0.02967792557347089 9.916829246229067E-4 34-35 0.0 0.0 0.0 0.05514724556439578 0.0010158703130283434 36-37 0.0 0.0 0.0 0.09713655183623396 0.0010158703130283434 38-39 0.0 0.0 0.0 0.16224900142366966 0.0010642450898392167 40-41 0.0 0.0 0.0 0.2353432891848995 0.0011126198666500903 42-43 0.0 0.0 0.0 0.30826826522729134 0.0011126198666500903 44-45 0.0 0.0 0.0 0.39976915556505854 0.0011851820318664005 46-47 0.0 0.0 0.0 0.5073304718040357 0.0012577441970827107 48-49 0.0 0.0 0.0 0.6233089992081049 0.001427055915920768 50-51 0.0 0.0 0.0 0.7544530191423829 0.001499618081137078 52-53 0.0 0.0 0.0 0.8838555471114695 0.001499618081137078 54-55 0.0 0.0 0.0 1.0182406770920762 0.0015479928579479516 56-57 0.0 0.0 0.0 1.1787481865505542 0.0015479928579479516 58-59 0.0 0.0 0.0 1.3526796965740497 0.0015721802463533885 60-61 0.0 0.0 0.0 1.5496617877479268 0.0015963676347588252 62-63 0.0 0.0 0.0 1.7407663435392826 0.001886616295624066 64-65 0.0 0.0 0.0 1.942706849336274 0.0019349910724349396 66-67 0.0 0.0 0.0 2.178412949347255 0.0019349910724349396 68-69 0.0 0.0 0.0 2.438451562094105 0.0019349910724349396 70-71 0.0 0.0 0.0 2.7210570082232284 0.001959178460840376 72-73 0.0 0.0 0.0 3.0128052871696065 0.0021043027912729965 74-75 0.0 0.0 0.0 3.3046261282812006 0.0022252397333001805 76-77 0.0 0.0 0.0 3.6229321596967483 0.0022252397333001805 78-79 0.0 0.0 0.0 3.977059713340748 0.002249427121705617 80-81 0.0 0.0 0.0 4.3525931057235585 0.0022978018985164907 82-83 0.0 0.0 0.0 4.7471377853930425 0.0023219892869219276 84-85 0.0 0.0 0.0 5.155493463842031 0.0023219892869219276 86-87 0.0 0.0 0.0 5.565590634256211 0.002491301005759985 88-89 0.0 0.0 0.0 6.004761045533726 0.002660612724598042 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3410 0.0 50.76581 1 GTATCAA 6115 0.0 42.852825 1 ATCAACG 7980 0.0 31.989424 3 TCAACGC 8150 0.0 31.32216 4 TATCAAC 8235 0.0 31.127878 2 CAACGCA 8275 0.0 30.791569 5 AACGCAG 8565 0.0 29.832563 6 AAGGTAA 1215 0.0 27.008291 1 ACGCAGA 9870 0.0 25.791899 7 TAAGGTG 1505 0.0 25.584888 5 CGCAGAG 10000 0.0 25.40911 8 AGGTAAG 1340 0.0 24.488863 2 GTAAGGT 1595 0.0 24.439268 4 TAGCGCG 160 1.8480732E-6 23.768677 4 TAGCCCT 1340 0.0 23.059164 4 GGTAAGG 1565 0.0 22.477726 3 GCAGAGT 11020 0.0 22.4539 9 TACATGG 6850 0.0 22.35577 2 GTACATG 7115 0.0 21.924175 1 TGGTATC 1680 0.0 21.797453 2 >>END_MODULE