FastQCFastQC Report
Thu 26 May 2016
SRR938616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075517
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.24779368224880838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37890.18255692437113258No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27810.1339907117118289No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26670.128498104327741No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26160.12604088523485957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.12131917011520502No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24660.11881377025579651No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24020.11573020119806295No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22960.11062303994619173No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22750.1096112438491229No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21480.10349228650018284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.051.0651441
GTATCAA58950.043.2177051
TATCAAC76350.032.9971242
ATCAACG77600.032.588113
TCAACGC78300.032.357484
CAACGCA79900.031.5310465
AACGCAG82950.030.4737916
ACGCAGA94550.026.62757
CGCAGAG94500.026.5919678
TAAGGTG13100.023.9486985
TGGTATC15150.023.8457092
GCAGAGT104250.023.784839
TACATGG73550.023.4602812
GTACATG74000.023.4034081
GAGTACT61200.021.34454312-13
ACATGGG75800.021.0707193
GTAAGGT13600.020.9711194
AGGTAAG12100.020.8209112
GGACCGA7550.020.768176
AGAGTAC94850.020.73256110-11
Produced by FastQC (version 0.11.5)