FastQCFastQC Report
Thu 26 May 2016
SRR938616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075517
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49900.24042202497016407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.1917594507778062No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29490.14208508048837953No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28950.13948331909591682No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28150.13562885777374986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.13182257721811No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26190.1261854275344408No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25090.12088554321646125No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24500.11804287799136312No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21520.1036850095662912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.047.513691
GTATCAA57950.043.4551431
ATCAACG75300.032.7366453
TCAACGC76700.032.077184
TATCAAC77450.032.0157172
CAACGCA78400.031.3210455
AACGCAG81400.029.991666
TAAGGTG14100.026.6115265
ACGCAGA93750.025.9900957
CGCAGAG96250.025.1669868
GTAAGGT14350.024.8239654
AAGGTAA13850.024.014141
AGGTAAG14850.023.991032
GGTAAGG15250.023.6703973
GCAGAGT107000.022.505369
ACCGAGT6900.022.027378
GGACCGA6700.021.9760026
GAGTACT60750.021.07047312-13
AGAGTAC95500.020.96312310-11
AGTACTT63400.020.86400812-13
Produced by FastQC (version 0.11.5)