##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938616_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2075517 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79775545081057 31.0 31.0 34.0 30.0 34.0 2 32.0571149260642 33.0 31.0 34.0 30.0 34.0 3 32.113569293819324 33.0 31.0 34.0 30.0 34.0 4 35.77134179098509 37.0 35.0 37.0 35.0 37.0 5 35.65842920101353 37.0 35.0 37.0 33.0 37.0 6 35.6906240710146 37.0 35.0 37.0 33.0 37.0 7 35.61350208165002 37.0 35.0 37.0 33.0 37.0 8 35.57887697378533 37.0 35.0 37.0 33.0 37.0 9 37.12187132169961 39.0 37.0 39.0 33.0 39.0 10-11 37.04772256743742 39.0 37.0 39.0 33.0 39.0 12-13 36.93111571719239 39.0 37.0 39.0 33.0 39.0 14-15 37.947545599481955 40.0 37.0 41.0 33.0 41.0 16-17 38.03608016701381 40.0 37.0 41.0 33.0 41.0 18-19 38.165608376129896 40.0 37.5 41.0 33.0 41.0 20-21 38.109419484398344 40.0 37.0 41.0 33.0 41.0 22-23 37.98686520033322 40.0 37.0 41.0 33.0 41.0 24-25 37.86294740057538 40.0 37.0 41.0 32.0 41.0 26-27 37.64449917779522 40.0 37.0 41.0 32.0 41.0 28-29 37.502615974718594 39.5 36.5 41.0 32.0 41.0 30-31 37.23874340706436 39.0 36.0 41.0 31.0 41.0 32-33 37.13449685066419 39.0 36.0 41.0 31.0 41.0 34-35 37.00446081626891 39.0 36.0 41.0 31.0 41.0 36-37 36.8635588145026 39.0 35.5 41.0 30.0 41.0 38-39 36.63709620301833 39.0 35.0 40.0 30.0 41.0 40-41 36.4068109295178 39.0 35.0 40.0 30.0 41.0 42-43 36.13591216068093 38.5 35.0 40.0 30.0 41.0 44-45 35.841524063642936 38.0 35.0 40.0 29.0 41.0 46-47 35.4360732771642 38.0 34.0 40.0 27.5 41.0 48-49 35.01972785575835 38.0 34.0 40.0 26.0 41.0 50-51 34.60969989645953 38.0 33.0 40.0 25.0 41.0 52-53 33.98693506244469 37.0 33.0 40.0 23.5 41.0 54-55 33.53731335373307 37.0 32.0 40.0 23.0 41.0 56-57 33.1326315322881 36.5 32.0 39.0 22.0 40.5 58-59 32.59087687549656 36.0 31.0 39.0 19.5 40.0 60-61 32.08090273411396 35.0 31.0 39.0 18.0 40.0 62-63 31.550592936603266 35.0 30.0 38.5 14.0 40.0 64-65 30.88042641905607 34.5 29.0 38.0 9.0 40.0 66-67 30.216010275993888 34.0 28.0 37.5 7.5 39.5 68-69 29.481556643477262 34.0 27.5 37.0 4.5 39.0 70-71 28.694305804288764 33.0 26.0 36.0 2.0 39.0 72-73 28.101216227089445 33.0 26.0 35.5 2.0 38.5 74-75 27.384119474810372 32.0 25.0 35.0 2.0 37.5 76-77 26.26869546238359 30.5 24.0 34.0 2.0 36.0 78-79 26.750015538297205 32.0 25.0 35.0 2.0 36.0 80-81 26.384694753162705 32.0 24.0 35.0 2.0 36.0 82-83 25.701270334090253 31.0 21.5 34.0 2.0 36.0 84-85 24.918312160295482 31.0 19.0 34.0 2.0 35.0 86-87 24.018067787447656 30.5 14.0 34.0 2.0 35.0 88-89 23.026106266535038 29.5 4.5 34.0 2.0 35.0 90-91 22.16429569114587 29.0 2.0 34.0 2.0 35.0 92-93 21.07881867505783 29.0 2.0 33.5 2.0 35.0 94-95 19.8932005856854 26.5 2.0 33.0 2.0 34.5 96-97 18.517238596455726 25.0 2.0 32.5 2.0 34.0 98-99 16.750527218037725 12.0 2.0 32.0 2.0 34.0 100-101 12.552410315116667 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3515914104433797 1101 2 0.3775249511450056 1101 3 0.3752631720234447 1101 4 0.27082894549531744 1101 5 0.25159070269351247 1101 6 0.24703766980668718 1101 7 0.28459952325306404 1101 8 0.2940231596641141 1101 9 0.44174625839687565 1101 10-11 0.3615772671714623 1101 12-13 0.38472497321841104 1101 14-15 0.5441470418244094 1101 16-17 0.5533623341117533 1101 18-19 0.5263710155487402 1101 20-21 0.5984224850978634 1101 22-23 0.5661080437312407 1101 24-25 0.5612006611069091 1101 26-27 0.6161305692429622 1101 28-29 0.689695572731452 1101 30-31 0.6558259330728546 1101 32-33 0.6208775277494922 1101 34-35 0.5816192347818472 1101 36-37 0.59681024598747 1101 38-39 0.8363676577357637 1101 40-41 0.8629827361957965 1101 42-43 0.8536339982127359 1101 44-45 0.8714595526509328 1101 46-47 0.9005553628347016 1101 48-49 0.9419040942041406 1101 50-51 1.104721927982439 1101 52-53 1.2170478984644362 1101 54-55 1.1019355638282633 1101 56-57 1.2589205034834023 1101 58-59 1.4191496148022935 1101 60-61 1.4112568991098016 1101 62-63 1.5080271389329987 1101 64-65 1.5319361312063968 1101 66-67 1.6140960218251656 1101 68-69 1.8498381621172157 1101 70-71 2.034043080781313 1101 72-73 2.020676879929571 1101 74-75 1.964992168812703 1101 76-77 2.002966232941354 1101 78-79 2.2634219352900473 1101 80-81 2.1856098932074133 1101 82-83 2.4344606348022886 1101 84-85 2.588308561712285 1101 86-87 2.4908385204359433 1101 88-89 2.4287565872703993 1101 90-91 2.6612153118647015 1101 92-93 3.0263374825225675 1101 94-95 2.9227643964101944 1101 96-97 3.0024525045503836 1101 98-99 2.97690595467623 1101 100-101 2.4739475644957967 1102 1 0.3458140955524378 1102 2 0.3503067182763857 1102 3 0.2977315769519677 1102 4 0.29902776757015204 1102 5 0.2702713534149481 1102 6 0.29783949942778776 1102 7 0.28252140500984524 1102 8 0.28303520081232136 1102 9 0.3267437307911578 1102 10-11 0.38395918535012896 1102 12-13 0.35763232885557983 1102 14-15 0.49401481454697915 1102 16-17 0.3808603698880404 1102 18-19 0.45570659022883575 1102 20-21 0.39838947395812596 1102 22-23 0.42321451379280717 1102 24-25 0.41545903722682453 1102 26-27 0.41664644443405763 1102 28-29 0.428410452715859 1102 30-31 0.4275876438051256 1102 32-33 0.32706437567289726 1102 34-35 0.39849730673638817 1102 36-37 0.46195036061287453 1102 38-39 0.36869189777912936 1102 40-41 0.5434998295101252 1102 42-43 0.5208032625272097 1102 44-45 0.5637785547643759 1102 46-47 0.4749668570455796 1102 48-49 0.6233558534786354 1102 50-51 0.7814756295544285 1102 52-53 0.6873652804739763 1102 54-55 0.6711939094950878 1102 56-57 0.7733853514920739 1102 58-59 0.8278042585926002 1102 60-61 0.7238375540946649 1102 62-63 1.0414101716944195 1102 64-65 1.243939152152027 1102 66-67 1.1773664390932765 1102 68-69 1.3269181989045364 1102 70-71 1.5169489137644376 1102 72-73 1.4044519656549959 1102 74-75 1.3388689069223005 1102 76-77 1.4224608336101028 1102 78-79 1.6806649569665488 1102 80-81 1.7214296596931895 1102 82-83 1.9167664202786021 1102 84-85 1.9391381645432055 1102 86-87 2.0446471654565634 1102 88-89 2.034589692205625 1102 90-91 2.4716072430163756 1102 92-93 2.6700648178170745 1102 94-95 2.1993252690736824 1102 96-97 2.4979685929130433 1102 98-99 2.3797964393065634 1102 100-101 2.1297517390041314 1103 1 0.3143487014125661 1103 2 0.2546777755220191 1103 3 0.25326038052372013 1103 4 0.15070753909374446 1103 5 0.08169734260570038 1103 6 0.16328425115853662 1103 7 0.21446291215877267 1103 8 0.2538849378113994 1103 9 0.30445065196317955 1103 10-11 0.3454439204512596 1103 12-13 0.36446565315107904 1103 14-15 0.5276489269414597 1103 16-17 0.36570067139012963 1103 18-19 0.38852332106410614 1103 20-21 0.45358857740514935 1103 22-23 0.4889745051164951 1103 24-25 0.3051707303743427 1103 26-27 0.41481718716311633 1103 28-29 0.4642812478643492 1103 30-31 0.40497571025127144 1103 32-33 0.49383407362853404 1103 34-35 0.5870076912057769 1103 36-37 0.43063354843710044 1103 38-39 0.4122473810320244 1103 40-41 0.6584838325468354 1103 42-43 0.6161057587765058 1103 44-45 0.728264934750996 1103 46-47 0.656770085359291 1103 48-49 0.6652628361079209 1103 50-51 0.5741271488140498 1103 52-53 0.6824848509961825 1103 54-55 0.9022139192559564 1103 56-57 0.8125054281328943 1103 58-59 0.9902661641291743 1103 60-61 1.13857066690489 1103 62-63 1.2244219389521582 1103 64-65 1.3077451641181206 1103 66-67 1.1041650581124927 1103 68-69 1.0726503537045318 1103 70-71 1.2901546329047626 1103 72-73 1.3634831100253244 1103 74-75 1.248982241340176 1103 76-77 1.3183677800880602 1103 78-79 1.485438378509123 1103 80-81 1.5499899329972386 1103 82-83 1.8361876374938682 1103 84-85 2.1753122693459055 1103 86-87 2.2760568865490534 1103 88-89 2.133523589499699 1103 90-91 2.17830644587956 1103 92-93 2.3530693548089268 1103 94-95 2.07240156645452 1103 96-97 2.050709767469737 1103 98-99 2.1223986120014615 1103 100-101 1.9990632369344663 1104 1 0.14442170659990694 1104 2 0.1487317474917944 1104 3 0.1684118041957703 1104 4 0.08784399398087572 1104 5 0.098912634496088 1104 6 0.07447758706243945 1104 7 0.12440183589371401 1104 8 0.06694161894554185 1104 9 0.16999664943926263 1104 10-11 0.24932278700239152 1104 12-13 0.22230667830655904 1104 14-15 0.31638236172552325 1104 16-17 0.2767370056615803 1104 18-19 0.40563867163857736 1104 20-21 0.2717244271556183 1104 22-23 0.3411679311276785 1104 24-25 0.21152080033326115 1104 26-27 0.40214808165391247 1104 28-29 0.41918324362610804 1104 30-31 0.3021547351076208 1104 32-33 0.4991494879641962 1104 34-35 0.4131430771553539 1104 36-37 0.4423504670610896 1104 38-39 0.3599490280740625 1104 40-41 0.45025049770409 1104 42-43 0.5218860260154727 1104 44-45 0.5575608218799744 1104 46-47 0.587267989793439 1104 48-49 0.7248094501555968 1104 50-51 0.7725737817522358 1104 52-53 0.9244005819422654 1104 54-55 0.8963235564804108 1104 56-57 1.126851397982179 1104 58-59 1.0572131238777516 1104 60-61 1.0086295751066174 1104 62-63 1.0008179956383252 1104 64-65 1.3624762991084118 1104 66-67 1.2849916025260022 1104 68-69 1.593554898084193 1104 70-71 1.6141458545352627 1104 72-73 1.6379527235200122 1104 74-75 1.5802118900178606 1104 76-77 1.6266793070038368 1104 78-79 1.6602982891428368 1104 80-81 1.8864655859662278 1104 82-83 1.9611461858611179 1104 84-85 2.217759313026896 1104 86-87 2.096745699479495 1104 88-89 2.094434185245998 1104 90-91 2.159066370372411 1104 92-93 2.562391752686967 1104 94-95 2.3774675162880925 1104 96-97 2.487820722455897 1104 98-99 2.3640203508156965 1104 100-101 2.047666048189919 1105 1 0.15432014253237725 1105 2 0.1533165925312403 1105 3 0.12994201414232265 1105 4 0.12730658082888624 1105 5 0.08071266281208267 1105 6 0.027516040349311766 1105 7 0.08934299335754048 1105 8 0.08290888357756643 1105 9 0.1825468864336699 1105 10-11 0.1642039249924565 1105 12-13 0.10819606528277603 1105 14-15 0.2502004688450796 1105 16-17 0.18310250080486412 1105 18-19 0.22285049347715358 1105 20-21 0.2525598878072586 1105 22-23 0.2838052528172881 1105 24-25 0.2513325598071745 1105 26-27 0.2286540188914259 1105 28-29 0.32429015385660165 1105 30-31 0.38055072118587674 1105 32-33 0.4426532268039125 1105 34-35 0.2675945504119426 1105 36-37 0.4068093406927318 1105 38-39 0.3615500540509444 1105 40-41 0.5201486470023156 1105 42-43 0.4154211643338641 1105 44-45 0.35884989425338887 1105 46-47 0.5596125623740065 1105 48-49 0.643452879760396 1105 50-51 0.6075397721647491 1105 52-53 0.6977143691363139 1105 54-55 0.6185000571626844 1105 56-57 0.6964407640253967 1105 58-59 0.8530326937005555 1105 60-61 0.7150490096033124 1105 62-63 0.8682718062669004 1105 64-65 1.032723577603118 1105 66-67 0.9079885285908667 1105 68-69 1.0922137972523487 1105 70-71 1.3108399193823494 1105 72-73 1.3193479026794428 1105 74-75 1.3008003935525814 1105 76-77 1.3170463551835745 1105 78-79 1.3815538687389974 1105 80-81 1.367354899112403 1105 82-83 1.577841718622981 1105 84-85 1.7848278177428973 1105 86-87 1.6863694963756792 1105 88-89 1.5908564687133655 1105 90-91 1.6678836306180678 1105 92-93 1.7702510899712216 1105 94-95 1.953073595713576 1105 96-97 1.7525613228069794 1105 98-99 1.9884274305078335 1105 100-101 1.9235647116926042 1106 1 -0.07906374265873595 1106 2 0.04927244390877661 1106 3 0.03917334506040504 1106 4 -0.012180036970988795 1106 5 0.08661599642626072 1106 6 0.02487049312544798 1106 7 0.08100193682371071 1106 8 -0.05292957515245433 1106 9 -0.19977962239886438 1106 10-11 0.109558770949711 1106 12-13 0.04668007123665774 1106 14-15 0.06864487584370238 1106 16-17 0.01871066773833263 1106 18-19 0.15510398295955952 1106 20-21 0.052708734944054925 1106 22-23 0.1606089711872727 1106 24-25 0.11154122664234478 1106 26-27 -0.11540052094624542 1106 28-29 0.09460919224792974 1106 30-31 0.2613129308637525 1106 32-33 0.157393306001822 1106 34-35 0.007955996195079251 1106 36-37 0.05736260269178217 1106 38-39 0.16926342501732705 1106 40-41 0.31110407711437915 1106 42-43 0.3692294042866777 1106 44-45 0.23564939996855117 1106 46-47 -0.2995906841786322 1106 48-49 -0.009820353030910667 1106 50-51 0.3869370816825395 1106 52-53 0.2500692431779399 1106 54-55 0.10979530809649418 1106 56-57 0.4005579319979873 1106 58-59 0.5501456209339679 1106 60-61 0.6977027012931458 1106 62-63 0.5430211533053999 1106 64-65 0.789949684829228 1106 66-67 0.5729617080218823 1106 68-69 0.4455783042313115 1106 70-71 0.3031925466747367 1106 72-73 0.6234608579972196 1106 74-75 0.4487109167643091 1106 76-77 0.6772704684197386 1106 78-79 1.0575431405113989 1106 80-81 1.1774203356838626 1106 82-83 1.269200159379011 1106 84-85 1.0881375602092653 1106 86-87 1.1226390501151151 1106 88-89 1.2278660454819779 1106 90-91 1.4026717340808688 1106 92-93 1.2982146926336178 1106 94-95 1.1581442278926417 1106 96-97 1.251737046681498 1106 98-99 1.32639749257428 1106 100-101 1.379446982032709 1107 1 0.06010278187364193 1107 2 0.03088595235155367 1107 3 0.06269941248891087 1107 4 0.078945098056586 1107 5 -0.041282121715759956 1107 6 -0.08050218297893252 1107 7 0.007762980657922469 1107 8 0.044608658971142745 1107 9 0.05120678956316027 1107 10-11 0.08137069885920312 1107 12-13 0.052058432712577485 1107 14-15 0.17010033095769472 1107 16-17 -0.05901973194328036 1107 18-19 -0.021574622987337477 1107 20-21 0.04592608669798892 1107 22-23 0.12640577600846115 1107 24-25 0.11040415233453871 1107 26-27 0.1404809959108846 1107 28-29 0.01593502089082932 1107 30-31 0.10252618915018274 1107 32-33 0.10946562392779668 1107 34-35 0.2757199395687735 1107 36-37 0.26395194830551816 1107 38-39 0.22791940318550274 1107 40-41 0.3151463762786193 1107 42-43 0.24120620797080505 1107 44-45 0.2662082719908412 1107 46-47 0.23097955858673203 1107 48-49 0.3746585826675428 1107 50-51 0.33727536128911595 1107 52-53 0.28078730560331167 1107 54-55 0.43262776019723503 1107 56-57 0.29896034259552096 1107 58-59 0.5167440631421627 1107 60-61 0.542190733586061 1107 62-63 0.7430723216492474 1107 64-65 0.6503851842891173 1107 66-67 0.6591178283243408 1107 68-69 0.8305802941113498 1107 70-71 0.6472086931299081 1107 72-73 0.8293338467970379 1107 74-75 0.7588081366176276 1107 76-77 0.8203371578278897 1107 78-79 1.0416695831744285 1107 80-81 1.3880519804618494 1107 82-83 1.540983660430804 1107 84-85 1.541074054609176 1107 86-87 1.8612484082366656 1107 88-89 1.639589281595459 1107 90-91 1.9071233799959266 1107 92-93 2.065159942505698 1107 94-95 1.8253908024562904 1107 96-97 1.8135882036546072 1107 98-99 1.7535053440723765 1107 100-101 1.3686732029662458 1108 1 -0.022438172780155696 1108 2 0.06048372258993595 1108 3 -0.018240459341349435 1108 4 0.07494328147762985 1108 5 -0.009288366947984628 1108 6 -3.9045743670129696E-4 1108 7 0.12140285674270501 1108 8 0.061009319509331306 1108 9 9.486988113849293E-4 1108 10-11 0.053856552596890594 1108 12-13 0.08543836782627778 1108 14-15 0.1045666515245145 1108 16-17 0.1251441717622015 1108 18-19 0.02334306204200942 1108 20-21 -0.015074753321364653 1108 22-23 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66-67 -0.01806577440894941 2316 68-69 0.17572470677548324 2316 70-71 0.10977751994186846 2316 72-73 0.21582823349656266 2316 74-75 0.36321490719766913 2316 76-77 0.4538094498843712 2316 78-79 0.21726955102777268 2316 80-81 -0.3846453093278761 2316 82-83 0.12006659467852998 2316 84-85 0.2325364389018283 2316 86-87 0.3889999352027651 2316 88-89 0.4662787325470852 2316 90-91 0.5617827903108825 2316 92-93 0.7828472998034002 2316 94-95 1.1922878878262644 2316 96-97 1.0677383161678975 2316 98-99 0.7912289281770946 2316 100-101 0.0772394621671939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 34.0 5 74.0 6 159.0 7 424.0 8 986.0 9 1792.0 10 3052.0 11 4776.0 12 6835.0 13 9293.0 14 12642.0 15 16733.0 16 20807.0 17 25894.0 18 25671.0 19 25163.0 20 26233.0 21 28735.0 22 31162.0 23 34744.0 24 39410.0 25 44478.0 26 51312.0 27 60385.0 28 70792.0 29 82503.0 30 95830.0 31 111360.0 32 129736.0 33 154861.0 34 187549.0 35 226360.0 36 256937.0 37 219797.0 38 67656.0 39 1323.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.18920405066171 19.01601864850394 15.294173209980247 23.500604090854107 2 16.047367216393006 20.225705027044608 38.94086325908614 24.786064497476236 3 19.40552432309504 23.481119511861376 29.71723113155729 27.396125033486296 4 13.644311272805766 16.933467661310413 35.90358450448732 33.518636561396505 5 14.155873752657778 37.09189005320612 33.96003783161079 14.792198362525314 6 33.27305919440795 35.859932730013774 16.711450689153594 14.155557386424682 7 29.483449183986448 32.075622603910254 19.921349716721185 18.519578495382113 8 27.054704924122518 34.63247952196971 19.2378091820014 19.075006371906372 9 26.388078513601215 16.581105490546193 19.489061258590045 37.54175473726254 10-11 25.279147316066307 26.19939995673367 27.047333266843875 21.474119460356142 12-13 26.378801040897283 23.27685102073363 27.209606088507105 23.134741849861985 14-15 22.807787168209174 24.96585188172393 25.3982983516878 26.828062598379105 16-17 22.732263816677964 28.01063542240319 26.357866497841258 22.899234263077588 18-19 22.77369927589126 26.488171380913766 28.381723686194814 22.35640565700016 20-21 23.14589569731301 25.663413019503096 28.758834545802326 22.43185673738158 22-23 23.01137499716938 25.57663945898781 28.645272479097976 22.76671306474483 24-25 22.952106753110908 25.893835186598956 28.549750286740828 22.604307773549305 26-27 22.45005048617792 26.290638000251043 28.295533147771636 22.963778365799403 28-29 22.60354428037791 26.616865324380047 28.15316020665734 22.6264301885847 30-31 22.668424301029575 26.083236128636866 28.11494678193433 23.133392788399227 32-33 22.37004113441861 26.076740082268834 28.146783023470107 23.40643575984245 34-35 23.196999959042163 26.282570513731716 27.860868643404224 22.659560883821896 36-37 23.439763391326743 25.648899575498756 27.874152400789303 23.0371846323852 38-39 22.66538971208288 26.143669437417177 27.847729189254306 23.343211661245633 40-41 23.243259652747795 25.901307014432863 27.973742135940295 22.881691196879046 42-43 22.983431590293172 25.915890500526277 27.76076520212361 23.33991270705694 44-45 22.862745617638094 26.213925991594827 27.974531530240192 22.948796860526883 46-47 23.35592128513553 26.501981554952707 27.135149250800943 23.006947909110817 48-49 23.045246076037923 26.538809366533734 27.098669873578483 23.317274683849856 50-51 23.31390203019296 26.6609476096799 27.048200520641362 22.976949839485776 52-53 23.59433818176387 26.30057956644055 27.138515367496385 22.96656688429919 54-55 23.05317181213163 26.487930477081136 27.328781214511856 23.130116496275384 56-57 23.114963645202614 26.598312613194686 27.126951983529885 23.159771758072807 58-59 23.07360045713911 26.278850040736838 27.055933533668963 23.591615968455088 60-61 22.741586795000956 26.568825984080114 27.364651795191268 23.32493542572766 62-63 22.79367020361674 26.59118185974868 27.44554730219025 23.169600634444336 64-65 22.54665827678515 26.54392931685422 27.259630858061325 23.649781548299316 66-67 22.491687581215018 26.542934769254195 27.250833410021347 23.71454423950944 68-69 23.125441459463783 26.17374013612129 27.325389231135404 23.375429173279525 70-71 22.857413357732074 26.340690054574356 26.861259146516264 23.940637441177305 72-73 22.87180979550362 26.38633764608132 26.891080207380003 23.850772351035058 74-75 22.905955480008114 26.79900959616327 26.64762562773516 23.647409296093453 76-77 22.913929396868348 26.28653487299791 27.065352873525008 23.734182856608737 78-79 22.922409211777115 26.554998104086835 27.052633151161853 23.469959532974194 80-81 23.197638366996497 26.612288142738688 26.705301291777022 23.484772198487796 82-83 23.052478514784653 26.62412906402659 26.79681059524538 23.526581825943378 84-85 22.95569490797769 26.631335214261775 26.736327351897366 23.676642525863166 86-87 23.212465516040957 26.62461577082053 26.409743755666014 23.753174957472496 88-89 22.9166034294106 26.829604382907966 26.19795453373786 24.05583765394357 90-91 22.87587163046843 26.7149363343881 26.4410736246939 23.96811841044957 92-93 22.9461243852695 26.306394160837154 26.664476033038735 24.083005420854608 94-95 22.693526480390187 26.16528797403249 26.75297287374664 24.38821267183068 96-97 22.92891361525827 25.804968111559674 26.725702559892305 24.540415713289747 98-99 22.6435630255016 26.07128729853815 26.43326939745615 24.8518802785041 100-101 22.969305697789295 25.711130560094585 26.574337797043672 24.74522594507244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 222.0 1 169.0 2 138.5 3 168.0 4 157.0 5 160.0 6 281.0 7 583.5 8 968.5 9 1156.5 10 1056.5 11 886.5 12 887.0 13 1018.5 14 1155.5 15 1330.5 16 1498.5 17 1710.5 18 1912.0 19 2157.5 20 2532.0 21 3037.5 22 3711.5 23 4528.0 24 5495.0 25 6944.5 26 9299.5 27 11821.5 28 14128.0 29 16899.5 30 20090.0 31 24866.5 32 31560.5 33 34137.0 34 34119.0 35 36706.5 36 40545.5 37 44450.0 38 48283.0 39 51643.0 40 55128.5 41 59841.0 42 63344.0 43 66241.5 44 69459.5 45 73975.0 46 76884.5 47 78838.0 48 80229.0 49 79017.0 50 78780.5 51 77781.5 52 76268.0 53 77391.0 54 79445.5 55 75925.0 56 69195.0 57 63597.0 58 58880.0 59 52133.5 60 42412.5 61 35447.5 62 30482.5 63 23521.5 64 16980.5 65 12907.0 66 9729.0 67 7514.0 68 5600.0 69 4088.5 70 3203.0 71 2295.5 72 1705.5 73 1410.0 74 1092.0 75 790.0 76 574.0 77 394.5 78 263.5 79 171.0 80 107.0 81 69.0 82 46.5 83 23.5 84 12.5 85 8.0 86 4.5 87 3.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02399402173048932 2 0.011659745499555051 3 0.0019754114276105666 4 0.0 5 1.9272306610834794E-4 6 0.0 7 0.0 8 0.0 9 1.9272306610834794E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001758597978238675 26-27 0.009467520622572593 28-29 1.9272306610834794E-4 30-31 0.0 32-33 2.1681344937189142E-4 34-35 0.01021432250374244 36-37 0.0010840672468594571 38-39 0.012864264662732227 40-41 0.012189733931353008 42-43 0.004408540137228459 44-45 4.5771728200732635E-4 46-47 2.4090383263543492E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.8180766527086985E-5 66-67 3.854461322166959E-4 68-69 7.227114979063048E-4 70-71 0.0 72-73 1.6863268284480446E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9272306610834794E-4 82-83 9.636153305417398E-4 84-85 0.005950324666095243 86-87 0.004601263203336807 88-89 0.0 90-91 0.0011804287799136311 92-93 6.745307313792178E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.227114979063047E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2075517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.82095198010198 #Duplication Level Percentage of deduplicated Percentage of total 1 75.37684401683134 40.568535022415176 2 13.730616659248323 14.779897197491845 3 4.632236839574813 7.479341895096462 4 2.1238127358851497 4.572224930912147 5 1.1254072459509685 3.028524467119295 6 0.6766684919478817 2.1851365446944984 7 0.43976784752733594 1.656810694291309 8 0.3220997589721491 1.3868572528353966 9 0.2351858667884028 1.1392134518535562 >10 1.2141909459039308 11.766032740409264 >50 0.07573551495272314 2.7634945387878465 >100 0.04087825518561052 4.162647459715753 >500 0.003412619271183965 1.3191886095556251 >1k 0.0031432019603010114 3.1920951948218375 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4990 0.24042202497016407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3980 0.1917594507778062 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2949 0.14208508048837953 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2895 0.13948331909591682 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2815 0.13562885777374986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2736 0.13182257721811 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2619 0.1261854275344408 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2509 0.12088554321646125 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2450 0.11804287799136312 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2152 0.1036850095662912 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4090383263543493E-4 2 0.0 0.0 0.0 0.0 2.4090383263543493E-4 3 0.0 0.0 0.0 0.0 2.4090383263543493E-4 4 0.0 0.0 0.0 0.0 2.4090383263543493E-4 5 0.0 0.0 0.0 0.0 2.4090383263543493E-4 6 0.0 0.0 0.0 0.0 2.4090383263543493E-4 7 0.0 0.0 0.0 0.0 2.4090383263543493E-4 8 0.0 0.0 0.0 0.0 2.4090383263543493E-4 9 0.0 0.0 0.0 0.0 2.4090383263543493E-4 10-11 0.0 0.0 0.0 0.0 2.4090383263543493E-4 12-13 0.0 0.0 0.0 0.0 4.336268987437829E-4 14-15 0.0 0.0 0.0 4.8180766527086985E-5 4.8180766527086986E-4 16-17 0.0 0.0 0.0 4.8180766527086985E-5 6.986211146427613E-4 18-19 0.0 0.0 0.0 4.8180766527086985E-5 7.227114979063047E-4 20-21 0.0 0.0 0.0 9.636153305417397E-5 8.190730309604788E-4 22-23 0.0 0.0 0.0 2.4090383263543493E-4 9.154345640146527E-4 24-25 0.0 0.0 0.0 7.227114979063047E-4 9.154345640146527E-4 26-27 0.0 0.0 0.0 0.001228609546440718 9.636153305417397E-4 28-29 0.0 0.0 0.0 0.003276292123841915 0.0010117960970688266 30-31 0.0 0.0 0.0 0.010816582085331029 0.0010117960970688266 32-33 0.0 0.0 0.0 0.025752619708727994 0.0011081576301230007 34-35 0.0 0.0 0.0 0.04880711649193911 0.0012526999297042616 36-37 0.0 0.0 0.0 0.0905075699211329 0.0012526999297042616 38-39 0.0 0.0 0.0 0.15856290264064327 0.0012526999297042616 40-41 0.0 0.0 0.0 0.22955726211830593 0.0012526999297042616 42-43 0.0 0.0 0.0 0.3034665579708574 0.0012526999297042616 44-45 0.0 0.0 0.0 0.39696133541666967 0.001324971079494892 46-47 0.0 0.0 0.0 0.508499809926876 0.0013972422292855226 48-49 0.0 0.0 0.0 0.6267113206010839 0.0014936037623396966 50-51 0.0 0.0 0.0 0.755835774893677 0.0015899652953938706 52-53 0.0 0.0 0.0 0.8798289775511354 0.0016381460619209576 54-55 0.0 0.0 0.0 1.0156505583909936 0.0016381460619209576 56-57 0.0 0.0 0.0 1.1761166013094568 0.001662236445184501 58-59 0.0 0.0 0.0 1.3534700028956639 0.0016863268284480445 60-61 0.0 0.0 0.0 1.547301226634135 0.001710417211711588 62-63 0.0 0.0 0.0 1.7367239102353775 0.0017826883615022185 64-65 0.0 0.0 0.0 1.9376377066533301 0.0017826883615022185 66-67 0.0 0.0 0.0 2.1686163013841853 0.0017826883615022185 68-69 0.0 0.0 0.0 2.4284792656480287 0.0018790498945563925 70-71 0.0 0.0 0.0 2.7060245712273137 0.001951321044347023 72-73 0.0 0.0 0.0 2.997542299099453 0.0021199537271918275 74-75 0.0 0.0 0.0 3.292288138328908 0.0022644960267730885 76-77 0.0 0.0 0.0 3.6025722747633484 0.002312676793300175 78-79 0.0 0.0 0.0 3.9633739449014387 0.0023608575598272623 80-81 0.0 0.0 0.0 4.339545279561671 0.0025053998594085232 82-83 0.0 2.4090383263543492E-5 0.0 4.731592176792578 0.0029149363748887623 84-85 0.0 4.8180766527086985E-5 0.0 5.135491542589147 0.00296311714141585 86-87 0.0 4.8180766527086985E-5 0.0 5.5481116271271205 0.003131749824260654 88-89 0.0 4.8180766527086985E-5 0.0 5.976631364619033 0.003228111357314828 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3280 0.0 47.51369 1 GTATCAA 5795 0.0 43.455143 1 ATCAACG 7530 0.0 32.736645 3 TCAACGC 7670 0.0 32.07718 4 TATCAAC 7745 0.0 32.015717 2 CAACGCA 7840 0.0 31.321045 5 AACGCAG 8140 0.0 29.99166 6 TAAGGTG 1410 0.0 26.611526 5 ACGCAGA 9375 0.0 25.990095 7 CGCAGAG 9625 0.0 25.166986 8 GTAAGGT 1435 0.0 24.823965 4 AAGGTAA 1385 0.0 24.01414 1 AGGTAAG 1485 0.0 23.99103 2 GGTAAGG 1525 0.0 23.670397 3 GCAGAGT 10700 0.0 22.50536 9 ACCGAGT 690 0.0 22.02737 8 GGACCGA 670 0.0 21.976002 6 GAGTACT 6075 0.0 21.070473 12-13 AGAGTAC 9550 0.0 20.963123 10-11 AGTACTT 6340 0.0 20.864008 12-13 >>END_MODULE