FastQCFastQC Report
Thu 26 May 2016
SRR938615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234676
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107900.3335728215128811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84830.26225192260368585No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66380.20521375247474555No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65760.20329702263843424No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63060.19494997335127226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55710.17222745029177575No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA54300.1678684356640356No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51620.15958321637159334No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA47370.14644434249365315No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46600.144063887696944No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45370.1402613430216813No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44170.1365515433384982No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42700.13200703872659889No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38780.11988835976153407No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35610.1100883055984587No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35270.10903719568822348No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33750.10433811608952488No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG32960.10189583129809601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58400.058.7179341
GTATCAA111300.045.7232361
TATCAAC149450.033.8595052
ATCAACG151850.033.355673
TCAACGC155150.032.6768574
CAACGCA155450.032.5832025
TGGTATC24350.031.6410522
AACGCAG161200.031.4556086
TAAGGTA11950.030.2468784
TAAGGTG23350.029.3298915
GTGTAAG13900.027.7152331
CGCAGAG183850.027.21348
ACGCAGA184800.027.176777
GTGGTAT31300.026.287551
AAGGTAA23350.025.8681351
GTAAGGT26500.025.843514
AGGTAAG23550.025.6476552
GTACATG147550.025.3033941
TACATGG143750.025.1774462
GGTAAGG27300.024.2151433
Produced by FastQC (version 0.11.5)