##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3234676 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.276570203630904 31.0 31.0 34.0 28.0 34.0 2 31.305490256211133 31.0 31.0 34.0 28.0 34.0 3 31.35177186215868 31.0 31.0 34.0 28.0 34.0 4 35.017985108864075 37.0 35.0 37.0 32.0 37.0 5 35.00621020466965 37.0 35.0 37.0 32.0 37.0 6 35.034747838732535 37.0 35.0 37.0 32.0 37.0 7 34.95457412117937 37.0 35.0 37.0 32.0 37.0 8 34.91562802580537 37.0 35.0 37.0 32.0 37.0 9 36.42833996356977 39.0 37.0 39.0 32.0 39.0 10-11 36.27137076479994 39.0 35.0 39.0 32.0 39.0 12-13 36.146732006544084 39.0 35.0 39.0 31.0 39.0 14-15 37.14242987551149 39.0 36.0 41.0 31.0 41.0 16-17 36.990352511348895 39.0 36.0 41.0 31.0 41.0 18-19 37.14563313296293 39.0 36.0 41.0 31.0 41.0 20-21 37.12154401244514 39.0 36.0 41.0 31.0 41.0 22-23 37.05297439372599 39.0 36.0 41.0 31.0 41.0 24-25 36.920260329009764 39.0 36.0 41.0 30.5 41.0 26-27 36.71705960658811 39.0 36.0 41.0 30.0 41.0 28-29 36.52551553849597 39.0 36.0 41.0 30.0 41.0 30-31 36.25728759232764 39.0 35.0 40.0 29.5 41.0 32-33 35.971978028093076 38.0 35.0 40.0 29.0 41.0 34-35 35.725822153439786 38.0 35.0 40.0 27.5 41.0 36-37 35.44220379413579 38.0 34.5 40.0 26.5 41.0 38-39 35.28837926889741 38.0 34.0 40.0 26.0 41.0 40-41 35.18016518501389 38.0 34.0 40.0 25.5 41.0 42-43 34.865168876264576 38.0 34.0 40.0 24.5 41.0 44-45 34.516553589911325 38.0 33.0 40.0 24.0 41.0 46-47 34.11765413290233 38.0 33.0 40.0 23.0 41.0 48-49 33.73272670895014 38.0 33.0 40.0 21.5 41.0 50-51 33.00340173173449 37.0 32.0 39.5 20.0 40.5 52-53 32.996472907951215 37.0 32.0 39.5 19.0 40.0 54-55 33.098316802053745 37.0 32.0 40.0 18.5 41.0 56-57 32.79178965683116 37.0 31.5 40.0 16.0 41.0 58-59 32.42007715765041 36.0 31.0 39.0 13.5 41.0 60-61 31.954927788749167 36.0 31.0 39.0 8.5 40.5 62-63 31.406791746684984 35.0 30.0 39.0 7.5 40.0 64-65 30.897923315967347 35.0 29.5 38.0 4.5 40.0 66-67 30.386501461042776 35.0 29.0 38.0 2.0 40.0 68-69 29.831810975813344 34.0 28.5 37.0 2.0 39.5 70-71 29.20880422026812 34.0 27.5 36.5 2.0 39.0 72-73 28.60310476226985 34.0 26.0 36.0 2.0 39.0 74-75 27.967301980167413 33.5 26.0 35.5 2.0 38.5 76-77 27.26051032622742 33.0 25.0 35.0 2.0 37.5 78-79 26.520773487050946 32.0 23.5 35.0 2.0 37.0 80-81 25.880259259350858 32.0 21.0 35.0 2.0 36.0 82-83 25.178610778946638 31.0 19.0 34.5 2.0 36.0 84-85 24.51943486766526 31.0 16.5 34.0 2.0 35.0 86-87 23.80781336368774 30.5 8.5 34.0 2.0 35.0 88-89 23.00095573714338 30.0 3.5 34.0 2.0 35.0 90-91 22.120411750666836 29.0 2.0 34.0 2.0 35.0 92-93 21.21357857788539 29.0 2.0 34.0 2.0 35.0 94-95 20.226567514026133 27.5 2.0 33.5 2.0 35.0 96-97 19.00194037980929 25.5 2.0 33.0 2.0 35.0 98-99 17.493908972645173 22.0 2.0 32.5 2.0 34.0 100-101 13.855142215170854 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4543165523272137 1101 2 0.314763212241985 1101 3 0.2575130700829149 1101 4 0.2507227946081727 1101 5 0.32358117397974695 1101 6 0.47383575038073644 1101 7 0.33819296242735675 1101 8 0.4156966460501792 1101 9 0.449590579794787 1101 10-11 0.5222541779435019 1101 12-13 0.539222706700123 1101 14-15 0.772907648853149 1101 16-17 0.5918034641351966 1101 18-19 0.5924291455972153 1101 20-21 0.6892508039878393 1101 22-23 0.7205252122500738 1101 24-25 0.6198719879787333 1101 26-27 0.8400438533170984 1101 28-29 0.8870385111053167 1101 30-31 0.7602637389239177 1101 32-33 0.7976401308535017 1101 34-35 0.7320383843731406 1101 36-37 0.7163138508579578 1101 38-39 0.7510693434127518 1101 40-41 0.90297979879238 1101 42-43 1.0122149093222035 1101 44-45 0.9540797774743979 1101 46-47 0.7695323523364337 1101 48-49 0.5665343181552913 1101 50-51 0.658502788522128 1101 52-53 0.6729994190497663 1101 54-55 0.7007800274092659 1101 56-57 0.659203352618519 1101 58-59 0.8374608917248736 1101 60-61 0.7950333669487222 1101 62-63 0.5620859969819172 1101 64-65 0.5914069079874515 1101 66-67 0.5534306063663799 1101 68-69 0.589479862452265 1101 70-71 0.6747049564978944 1101 72-73 0.7909927557680767 1101 74-75 0.5238976429301729 1101 76-77 0.5904962549286381 1101 78-79 0.8144409542023041 1101 80-81 0.7654932060513069 1101 82-83 1.0192239449338238 1101 84-85 1.1475766273035006 1101 86-87 1.1785344930738297 1101 88-89 0.9842937014728257 1101 90-91 0.9066553900929009 1101 92-93 0.9898849399118781 1101 94-95 0.8114178365297811 1101 96-97 0.5739198578896385 1101 98-99 0.703744817295874 1101 100-101 0.820748366698961 1102 1 0.4017433053174493 1102 2 0.28863470451576134 1102 3 0.35383151625870823 1102 4 0.29782882694957635 1102 5 0.24148616485192065 1102 6 0.39743091413281917 1102 7 0.4389780546532478 1102 8 0.4580911274603494 1102 9 0.6169094948312477 1102 10-11 0.585423925457043 1102 12-13 0.6372846661386902 1102 14-15 0.5504307676744133 1102 16-17 0.6250758622078791 1102 18-19 0.6077688700667565 1102 20-21 0.4721134477634692 1102 22-23 0.5577120981174133 1102 24-25 0.48094059127272715 1102 26-27 0.4636198137332883 1102 28-29 0.4139359004492178 1102 30-31 0.5948806199031367 1102 32-33 0.5344701339459874 1102 34-35 0.4559996584770687 1102 36-37 0.6192352518535387 1102 38-39 0.4179278771756998 1102 40-41 0.4065214425979278 1102 42-43 0.5269640688645225 1102 44-45 0.5671358013364056 1102 46-47 0.5450760311968068 1102 48-49 0.6673692891757099 1102 50-51 0.7086940736991494 1102 52-53 0.430663046435221 1102 54-55 0.4166010822460109 1102 56-57 0.46744806796023397 1102 58-59 0.542109844768973 1102 60-61 0.6260003671382535 1102 62-63 0.3988808355242561 1102 64-65 0.5355658517541002 1102 66-67 0.35007426228250793 1102 68-69 0.37571356455615046 1102 70-71 0.6308360579214316 1102 72-73 0.6987329471031316 1102 74-75 0.6686508925336376 1102 76-77 0.7258500950970266 1102 78-79 0.7959937808335908 1102 80-81 0.9606948660175902 1102 82-83 0.9222171713935268 1102 84-85 0.8312577820973495 1102 86-87 1.0302493260297432 1102 88-89 1.0026488484656362 1102 90-91 0.9494484030713615 1102 92-93 1.0883020363674873 1102 94-95 0.9641277514365569 1102 96-97 1.0498231628585621 1102 98-99 1.1902072136726751 1102 100-101 1.2414478170346452 1103 1 0.3341069653460842 1103 2 0.36280613376333193 1103 3 0.3005580553710345 1103 4 0.22910212218226889 1103 5 0.2398698454691086 1103 6 0.4438551328920681 1103 7 0.39204267193106546 1103 8 0.35604883810841415 1103 9 0.5311191430510505 1103 10-11 0.5276384368339961 1103 12-13 0.49490035839905033 1103 14-15 0.5552507361999588 1103 16-17 0.681956460459034 1103 18-19 0.7366617622845197 1103 20-21 0.658703676485672 1103 22-23 0.7463351501922446 1103 24-25 0.7001722479409338 1103 26-27 0.5210325786333669 1103 28-29 0.6953440861721205 1103 30-31 0.7033846370365993 1103 32-33 0.7629737369419729 1103 34-35 0.7670390843642139 1103 36-37 0.8827426412492514 1103 38-39 0.8926243735727084 1103 40-41 0.9438668805778789 1103 42-43 1.0675362293605346 1103 44-45 0.8696700268401827 1103 46-47 0.8927423665534988 1103 48-49 0.7669125547088242 1103 50-51 1.0279680315517794 1103 52-53 0.9198939931534795 1103 54-55 0.9501000705673306 1103 56-57 0.9372796323349419 1103 58-59 0.8506298357762745 1103 60-61 1.0312909733171907 1103 62-63 0.9977502402312304 1103 64-65 1.1382281640610792 1103 66-67 1.0997536015557543 1103 68-69 1.2561609513487184 1103 70-71 1.082499115675862 1103 72-73 1.106479092666536 1103 74-75 1.1076185661459839 1103 76-77 1.2451941605070829 1103 78-79 1.3587146048488314 1103 80-81 1.4818685316074962 1103 82-83 1.4906504301821144 1103 84-85 1.7172318334267338 1103 86-87 1.7750329687829307 1103 88-89 1.4205721265411988 1103 90-91 1.3993348110795978 1103 92-93 1.4810132168959278 1103 94-95 1.5181697331435124 1103 96-97 1.7602511443525337 1103 98-99 1.702431368536427 1103 100-101 1.3058387983037996 1104 1 0.38048324633809116 1104 2 0.3590443793113991 1104 3 0.35763018672404456 1104 4 0.22712311940176733 1104 5 0.24338686324384895 1104 6 0.3817543554738094 1104 7 0.29340059767228155 1104 8 0.3836490003866473 1104 9 0.5581246149937371 1104 10-11 0.5804862218351374 1104 12-13 0.5485067611327068 1104 14-15 0.6846326606482336 1104 16-17 0.7591116769454516 1104 18-19 0.6734814388494996 1104 20-21 0.6356357604085829 1104 22-23 0.6896965853650627 1104 24-25 0.656339315355666 1104 26-27 0.7169703455925927 1104 28-29 0.7729620594753754 1104 30-31 0.8636888097639641 1104 32-33 0.8054226869160814 1104 34-35 0.7928212027547517 1104 36-37 0.9695356012766894 1104 38-39 1.066528253407654 1104 40-41 0.9635152447671729 1104 42-43 0.9200634159112369 1104 44-45 0.8760155343389471 1104 46-47 0.8062099752254852 1104 48-49 0.6493937624282609 1104 50-51 0.7999959816677205 1104 52-53 0.844031890067221 1104 54-55 0.9278291978600919 1104 56-57 0.9908767997223009 1104 58-59 0.9823375832423267 1104 60-61 0.984848065898241 1104 62-63 1.0008153857044242 1104 64-65 1.0325428189065065 1104 66-67 1.0440311500990411 1104 68-69 1.2629759772571632 1104 70-71 1.4545282202100864 1104 72-73 1.307547589251108 1104 74-75 1.3678851378205152 1104 76-77 1.369072808056579 1104 78-79 1.3354080540197941 1104 80-81 1.3501715037398405 1104 82-83 1.466056373127465 1104 84-85 1.4939048329022988 1104 86-87 1.5048352664051592 1104 88-89 1.4831265903468847 1104 90-91 1.3978969006426674 1104 92-93 1.4475138122459335 1104 94-95 1.6033374371374727 1104 96-97 1.7395878815706496 1104 98-99 1.8857124232129756 1104 100-101 1.6186802175126598 1105 1 0.46070550061740434 1105 2 0.30660575939383605 1105 3 0.3001653847937149 1105 4 0.3904903716035335 1105 5 0.3411252354587333 1105 6 0.5555235172918387 1105 7 0.48730654463259526 1105 8 0.49024745942693215 1105 9 0.5322489940417157 1105 10-11 0.6291442626783308 1105 12-13 0.5770190778370434 1105 14-15 0.6511061833403389 1105 16-17 0.5846760975196901 1105 18-19 0.6027980186537789 1105 20-21 0.5970476533428339 1105 22-23 0.6785869538120082 1105 24-25 0.758148629913407 1105 26-27 0.5957587848191253 1105 28-29 0.6830576389257885 1105 30-31 0.8188813187615196 1105 32-33 0.830832863562982 1105 34-35 0.8749490045137023 1105 36-37 1.0469429963544528 1105 38-39 1.0048029407585517 1105 40-41 1.102622984951843 1105 42-43 0.9752546302955025 1105 44-45 0.9294050785487613 1105 46-47 0.9185602162085047 1105 48-49 0.9833981369882423 1105 50-51 1.0997066664195216 1105 52-53 1.1706480298262747 1105 54-55 1.265685046984629 1105 56-57 1.1963667615373694 1105 58-59 1.0992017092612159 1105 60-61 1.1694318723819066 1105 62-63 1.3535182916797375 1105 64-65 1.4388162067122359 1105 66-67 1.5574374872927912 1105 68-69 1.5568149742154347 1105 70-71 1.5950396911004 1105 72-73 1.5004048994392214 1105 74-75 1.5753792843189558 1105 76-77 1.8314439477971405 1105 78-79 1.8036561216553224 1105 80-81 1.8428350534708713 1105 82-83 2.0431616917687876 1105 84-85 1.920660236063373 1105 86-87 2.1268932576831645 1105 88-89 1.9668684050001808 1105 90-91 2.016435084953681 1105 92-93 2.072756860480755 1105 94-95 2.1199738345122405 1105 96-97 2.3439392618133716 1105 98-99 2.391290665325389 1105 100-101 1.89319772746577 1106 1 0.18396041485995696 1106 2 0.15280731549388094 1106 3 0.02644468448728432 1106 4 0.10475810187134016 1106 5 0.1925189091198689 1106 6 0.008429076634271837 1106 7 0.31052891218907064 1106 8 0.21018322577857163 1106 9 0.15658347572038878 1106 10-11 0.12689362863203968 1106 12-13 0.08239388670202885 1106 14-15 0.38764843569383345 1106 16-17 0.12232432311120789 1106 18-19 0.0388468509646529 1106 20-21 0.20723660834857327 1106 22-23 0.2021725546668165 1106 24-25 0.0803562163617002 1106 26-27 0.026158003698661503 1106 28-29 0.261875887177176 1106 30-31 -0.0014993546478336839 1106 32-33 0.18011100262944524 1106 34-35 -0.09784687863755437 1106 36-37 -0.06036741919918143 1106 38-39 0.17972909662647396 1106 40-41 0.29500767449944476 1106 42-43 -0.4285929406777669 1106 44-45 -0.13239370924070215 1106 46-47 -0.026993169567980146 1106 48-49 0.32922356393321195 1106 50-51 0.08395529853757466 1106 52-53 0.019726590157397084 1106 54-55 0.1264911608489001 1106 56-57 -0.827155895254748 1106 58-59 -0.41448171587833826 1106 60-61 0.260714580266189 1106 62-63 0.19746153762829266 1106 64-65 0.32417664590827044 1106 66-67 -0.12184716111088889 1106 68-69 -0.08689399700223888 1106 70-71 -0.10996298018421058 1106 72-73 0.1302799938553143 1106 74-75 0.43217233038172864 1106 76-77 0.38128336367623916 1106 78-79 0.616618606993967 1106 80-81 0.5470670437928362 1106 82-83 0.38962730588790606 1106 84-85 0.36397987522502007 1106 86-87 0.2294260653568152 1106 88-89 0.4810630587464253 1106 90-91 0.6572087135407223 1106 92-93 0.5843631315516653 1106 94-95 0.4688374638975965 1106 96-97 0.49361563721790347 1106 98-99 0.79952282759211 1106 100-101 0.49383607237467153 1107 1 0.3060092898548312 1107 2 0.16562483338480405 1107 3 0.17033338599648573 1107 4 0.22341284627054847 1107 5 0.22604774106277148 1107 6 0.3905133014740798 1107 7 0.2710713267256466 1107 8 0.23447445186037896 1107 9 0.3578024223630081 1107 10-11 0.49859118617127507 1107 12-13 0.4926785937142384 1107 14-15 0.49588041197583266 1107 16-17 0.5003439135719674 1107 18-19 0.5086795981695502 1107 20-21 0.546723597530189 1107 22-23 0.5974750491538501 1107 24-25 0.6283465777705715 1107 26-27 0.5153402749005096 1107 28-29 0.6861105942314225 1107 30-31 0.5940338674906229 1107 32-33 0.6431849577443174 1107 34-35 0.6026582712630315 1107 36-37 0.6409016856285348 1107 38-39 0.6860223609799974 1107 40-41 0.655751179458143 1107 42-43 0.708250684128167 1107 44-45 0.6452320787541908 1107 46-47 0.7821631090458894 1107 48-49 0.7240745880522041 1107 50-51 0.6621767079750995 1107 52-53 0.7687796792804562 1107 54-55 0.8092983640873399 1107 56-57 0.9110026866192342 1107 58-59 0.8452258310791123 1107 60-61 0.7804729822058576 1107 62-63 0.8790492057761305 1107 64-65 0.8134693678622078 1107 66-67 0.863041774936864 1107 68-69 1.0085261590597696 1107 70-71 1.1520849551619783 1107 72-73 1.1630326348892517 1107 74-75 1.10816049566537 1107 76-77 1.3013574165855601 1107 78-79 1.377723064076445 1107 80-81 1.4979925370313367 1107 82-83 1.2961230150279732 1107 84-85 1.3880389445674517 1107 86-87 1.572257213757883 1107 88-89 1.6622667879374369 1107 90-91 1.522367446449696 1107 92-93 1.5138566487696998 1107 94-95 1.6694338232353196 1107 96-97 1.9083022620474743 1107 98-99 1.8085500399486953 1107 100-101 1.4469974344318608 1108 1 0.18937697209909743 1108 2 0.049945814930008936 1108 3 0.13666467711877672 1108 4 0.07725642476282957 1108 5 0.11544897334388082 1108 6 0.13297974497313447 1108 7 0.17929237411554766 1108 8 0.25611029812526453 1108 9 0.17558280955837802 1108 10-11 0.3045279529691456 1108 12-13 0.17723705306416804 1108 14-15 0.25489259881785387 1108 16-17 0.3974064464218685 1108 18-19 0.34739146720055913 1108 20-21 0.28546078801663555 1108 22-23 0.22721046223461627 1108 24-25 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-1.663018839000216 2316 56-57 -1.5254521938987295 2316 58-59 -1.4661982503986692 2316 60-61 -1.499987620148481 2316 62-63 -1.5848155471142604 2316 64-65 -1.7250731269067998 2316 66-67 -1.649340788636497 2316 68-69 -1.7440537570847354 2316 70-71 -1.8665553347796724 2316 72-73 -1.7153287368158026 2316 74-75 -1.7371424920068783 2316 76-77 -1.8354177283362318 2316 78-79 -2.101258705400543 2316 80-81 -2.218257929848285 2316 82-83 -2.3526928535802227 2316 84-85 -2.373252243945938 2316 86-87 -2.2308094469586734 2316 88-89 -2.6306729278971837 2316 90-91 -2.6031940729843193 2316 92-93 -2.6608302995606685 2316 94-95 -3.0761333766782535 2316 96-97 -3.204097680509131 2316 98-99 -3.032699296080633 2316 100-101 -2.736156066463961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9397.0 3 2972.0 4 3173.0 5 3699.0 6 4566.0 7 5765.0 8 7611.0 9 9627.0 10 12535.0 11 15805.0 12 20122.0 13 23759.0 14 27735.0 15 32373.0 16 38080.0 17 43879.0 18 41330.0 19 38155.0 20 39880.0 21 43076.0 22 47111.0 23 53147.0 24 59335.0 25 66586.0 26 75245.0 27 85701.0 28 98387.0 29 112984.0 30 130697.0 31 153150.0 32 182428.0 33 218989.0 34 268829.0 35 331707.0 36 388729.0 37 369437.0 38 160817.0 39 7858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.294410396076486 18.021265432680845 14.362746568119288 23.321577603123377 2 15.823566129742547 19.64672499394208 39.762145781877116 24.76756309443826 3 19.520830315658113 23.633689511860855 29.371543601228613 27.473936571252416 4 13.090304698852794 15.93580107060118 35.91209246973362 35.06180176081241 5 13.908446833749435 37.31089241718033 33.557181017823694 15.22347973124654 6 33.225747256477675 35.28527278640615 16.774237270932172 14.714742686183998 7 29.536036296515967 32.72274274702062 19.573017454030218 18.1682035024332 8 27.535896656469163 33.86721553641122 19.227552007748166 19.369335799371452 9 27.150652179425254 16.379440964326335 19.446889013539582 37.02301784270883 10-11 25.483325378823846 26.125831163757624 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36-37 23.232415246143944 25.40285755778301 28.32249514411902 23.04223205195402 38-39 22.408552943472184 25.89526624272841 28.2831119166687 23.4130688971307 40-41 23.09209498817828 25.71122839192134 28.460609471911624 22.736067147988763 42-43 22.679857149660716 25.757005981579628 28.196969069091544 23.36616779966811 44-45 22.517625300170387 26.16688097654814 28.525268727277563 22.79022499600391 46-47 23.280297803284906 26.54306739918846 27.45865264698657 22.71798215054006 48-49 22.804024728891033 26.625668274767033 27.291272493475898 23.27903450286603 50-51 23.189280454960453 26.720316127518284 27.342102423609266 22.74830099391199 52-53 23.564966779172824 26.09317847234128 27.52275849659042 22.81909625189548 54-55 22.6432193012385 26.745323030987272 27.68526962496593 22.9261880428083 56-57 22.838752266799826 26.86874745878792 27.370744097817536 22.92175617659472 58-59 22.738232156078684 26.150455058393764 27.395061062254882 23.71625172327267 60-61 22.124916856403626 26.61581520305591 27.85711701454267 23.40215092599779 62-63 22.483755291743087 26.584863914238827 28.05562220262661 22.875758591391477 64-65 22.15114681937701 26.721836803186783 27.69776448714637 23.429251890289837 66-67 21.99423617580375 26.651951528470825 27.745832110764855 23.607980184960567 68-69 22.847386755703607 26.13919864898666 27.887127807358166 23.12628678795157 70-71 22.590647265208666 26.3515854796146 27.315597163536403 23.74217009164033 72-73 22.461956836018494 26.511730458744477 27.379265196160134 23.647047509076895 74-75 22.472134573538796 26.979818700586 27.31310446216368 23.234942263711527 76-77 22.757681933282807 26.36585826670365 27.402417933164063 23.47404186684948 78-79 22.76319393732157 26.712362860405914 27.355480544310396 23.16896265796212 80-81 22.97085224669391 26.658640661916603 27.034527278831938 23.335979812557543 82-83 22.742382682552208 26.715071269746655 27.251758555955963 23.290787491745174 84-85 22.578753251483118 26.914821538077693 27.21624505659401 23.290180153845178 86-87 22.99866721847522 26.897544024227223 26.677521443776055 23.42626731352151 88-89 22.700298938406814 26.911090410775884 26.621600140036534 23.76701051078077 90-91 22.570503443604977 26.813668034624698 26.80360163660149 23.81222688516884 92-93 22.768210839204308 26.32711508096283 27.070952028415697 23.833722051417165 94-95 22.51382986241972 26.058737808449116 27.375371949879536 24.05206037925163 96-97 22.85712474196083 25.5421702770114 27.34421114667357 24.256493834354202 98-99 22.50262729976667 25.91159996332526 26.942806424635034 24.642966312273035 100-101 22.985345449679752 25.248134246107035 27.424528717798392 24.34199158641482 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 595.0 1 434.0 2 372.5 3 484.0 4 454.5 5 469.5 6 757.0 7 1588.0 8 2593.5 9 2994.0 10 2638.5 11 2263.5 12 2289.5 13 2507.0 14 2761.0 15 3001.5 16 3306.5 17 3592.0 18 3966.5 19 4574.0 20 5194.0 21 5985.5 22 7174.5 23 8647.0 24 10541.5 25 13285.5 26 16928.5 27 21003.0 28 24517.5 29 28159.5 30 32552.5 31 40993.0 32 55072.0 33 58696.0 34 54263.0 35 56552.5 36 62501.0 37 69032.5 38 74815.0 39 79276.5 40 84556.5 41 91418.5 42 95322.0 43 99306.5 44 103991.5 45 110650.0 46 115930.5 47 120238.5 48 121463.5 49 118618.5 50 118915.0 51 117075.0 52 114502.0 53 116845.0 54 121583.0 55 116617.0 56 106225.5 57 98768.0 58 93385.5 59 83286.5 60 66991.0 61 56311.0 62 49227.5 63 37527.5 64 26639.5 65 20334.5 66 15127.0 67 11444.5 68 9073.0 69 7233.5 70 5589.5 71 3811.0 72 2717.0 73 2130.0 74 1542.5 75 1115.5 76 848.0 77 590.5 78 405.0 79 269.0 80 164.0 81 105.5 82 68.5 83 46.5 84 29.5 85 26.0 86 21.5 87 13.0 88 7.0 89 5.0 90 4.5 91 3.5 92 4.0 93 4.0 94 3.5 95 2.5 96 1.5 97 2.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006955874405968325 2 0.10415262610536573 3 0.11475647019979746 4 0.11688960501762774 5 0.1576355715379222 6 0.09753681667035585 7 0.02680330271099795 8 0.027174282679316257 9 0.02318624801989442 10-11 0.052833730487999414 12-13 0.054348565358632514 14-15 0.02791624261595288 16-17 0.031347807322897256 18-19 0.03706708183447121 20-21 0.05855300499957337 22-23 0.031548754805736344 24-25 0.05014412571769166 26-27 0.056914510139500836 28-29 0.05388484039823462 30-31 0.06844580415472833 32-33 0.0504223606939304 34-35 0.06254103965899521 36-37 0.05380755290483498 38-39 0.03181153228329515 40-41 0.03774721177638811 42-43 0.03345002714336768 44-45 0.008130644305642976 46-47 0.014236356284215174 48-49 0.00993917165119474 50-51 0.00881077424755988 52-53 0.010341066616872912 54-55 0.012257796453184184 56-57 0.01517926370369088 58-59 0.019801055808989835 60-61 0.025133892853565553 62-63 0.012118678965064817 64-65 0.009815511661755304 66-67 0.011283974036348617 68-69 0.0112221440416289 70-71 0.030961369855899014 72-73 0.02828722258427119 74-75 0.029817514953584222 76-77 0.023402653001413434 78-79 0.006121169477252126 80-81 0.005873849498373253 82-83 0.0063066594614112825 84-85 0.005889306997053183 86-87 0.014298186278934892 88-89 0.038149106742066285 90-91 0.03542858697439867 92-93 0.02998754743906345 94-95 0.047779128419662434 96-97 0.043203708810403266 98-99 0.0260458852756814 100-101 0.07430419615442165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3234676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.3136611361936 #Duplication Level Percentage of deduplicated Percentage of total 1 71.68290259418785 31.048489522234263 2 13.930111042497334 12.067282185685563 3 5.429420828355369 7.055042817755281 4 2.7536829551773048 4.770883615882479 5 1.6260918793057608 3.5215996318282974 6 1.005499486390608 2.6131118415683705 7 0.663596252454911 2.0119948247056 8 0.4673542248216091 1.6194258019593293 9 0.36520603867594675 1.4236569543691413 >10 1.837642940445642 14.466999571929737 >50 0.1442094849183821 4.297952832783891 >100 0.08180928489880397 6.77857547429514 >500 0.006953332318847417 2.0142349541001825 >1k 0.0049461848449279385 4.604426906918446 >5k 5.017868683260228E-4 1.3713054888261322 >10k+ 7.168383833228896E-5 0.3350175751581299 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10790 0.3335728215128811 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8483 0.26225192260368585 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6638 0.20521375247474555 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6576 0.20329702263843424 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6306 0.19494997335127226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5571 0.17222745029177575 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5430 0.1678684356640356 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5162 0.15958321637159334 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4737 0.14644434249365315 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4660 0.144063887696944 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4537 0.1402613430216813 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4417 0.1365515433384982 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4270 0.13200703872659889 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3878 0.11988835976153407 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3561 0.1100883055984587 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3527 0.10903719568822348 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3375 0.10433811608952488 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3296 0.10189583129809601 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.182999471971845E-5 0.0 5 0.0 0.0 0.0 6.182999471971845E-5 0.0 6 0.0 0.0 0.0 6.182999471971845E-5 0.0 7 0.0 0.0 0.0 6.182999471971845E-5 0.0 8 0.0 0.0 0.0 6.182999471971845E-5 0.0 9 0.0 0.0 0.0 6.182999471971845E-5 0.0 10-11 0.0 0.0 0.0 6.182999471971845E-5 0.0 12-13 0.0 0.0 0.0 6.182999471971845E-5 0.0 14-15 0.0 0.0 0.0 6.182999471971845E-5 0.0 16-17 0.0 0.0 0.0 1.8548998415915535E-4 0.0 18-19 0.0 0.0 0.0 2.1640498151901457E-4 0.0 20-21 0.0 0.0 0.0 2.6277747755880344E-4 0.0 22-23 0.0 0.0 0.0 5.56469952477466E-4 0.0 24-25 0.0 0.0 0.0 9.274499207957768E-4 0.0 26-27 0.0 0.0 0.0 0.001638494860072539 0.0 28-29 0.0 0.0 0.0 0.00519371955645635 0.0 30-31 0.0 0.0 0.0 0.0163385761046856 0.0 32-33 0.0 0.0 0.0 0.03937024913778073 0.0 34-35 0.0 0.0 0.0 0.07362406621250475 0.0 36-37 0.0 0.0 0.0 0.13004393639424783 0.0 38-39 0.0 0.0 0.0 0.22076399614675474 0.0 40-41 0.0 0.0 0.0 0.3176979703685933 0.0 42-43 0.0 0.0 0.0 0.4158840019835062 0.0 44-45 0.0 0.0 0.0 0.538261019032509 0.0 46-47 0.0 0.0 0.0 0.686282026391515 0.0 48-49 0.0 0.0 0.0 0.8416762606208473 0.0 50-51 0.0 0.0 0.0 1.0118324060895125 0.0 52-53 0.0 0.0 0.0 1.1767020870096419 0.0 54-55 0.0 0.0 0.0 1.3563955091638236 0.0 56-57 0.0 0.0 0.0 1.5602490017547352 0.0 58-59 0.0 0.0 0.0 1.7788644055849798 0.0 60-61 0.0 0.0 0.0 2.027637389339767 0.0 62-63 0.0 0.0 0.0 2.270134628630503 0.0 64-65 0.0 0.0 0.0 2.5170527125436983 0.0 66-67 0.0 0.0 0.0 2.8095858750613663 0.0 68-69 0.0 0.0 0.0 3.134703444796326 0.0 70-71 0.0 0.0 0.0 3.468709076272245 0.0 72-73 0.0 0.0 0.0 3.8270293531716932 0.0 74-75 0.0 0.0 0.0 4.177651795728536 0.0 76-77 0.0 0.0 0.0 4.553037151170628 0.0 78-79 0.0 0.0 0.0 4.974284905196069 0.0 80-81 0.0 0.0 0.0 5.41774508482457 0.0 82-83 0.0 0.0 0.0 5.878873185444229 0.0 84-85 0.0 0.0 0.0 6.347900067889334 0.0 86-87 0.0 0.0 0.0 6.824779359663843 0.0 88-89 0.0 0.0 0.0 7.327302641748354 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5840 0.0 58.717934 1 GTATCAA 11130 0.0 45.723236 1 TATCAAC 14945 0.0 33.859505 2 ATCAACG 15185 0.0 33.35567 3 TCAACGC 15515 0.0 32.676857 4 CAACGCA 15545 0.0 32.583202 5 TGGTATC 2435 0.0 31.641052 2 AACGCAG 16120 0.0 31.455608 6 TAAGGTA 1195 0.0 30.246878 4 TAAGGTG 2335 0.0 29.329891 5 GTGTAAG 1390 0.0 27.715233 1 CGCAGAG 18385 0.0 27.2134 8 ACGCAGA 18480 0.0 27.17677 7 GTGGTAT 3130 0.0 26.28755 1 AAGGTAA 2335 0.0 25.868135 1 GTAAGGT 2650 0.0 25.84351 4 AGGTAAG 2355 0.0 25.647655 2 GTACATG 14755 0.0 25.303394 1 TACATGG 14375 0.0 25.177446 2 GGTAAGG 2730 0.0 24.215143 3 >>END_MODULE