FastQCFastQC Report
Thu 26 May 2016
SRR938615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234676
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104180.3220724424950134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87660.27100086685652597No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65380.20212225273875964No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65000.20094748283908498No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA60510.18706664902450817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59000.18239848442316944No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC57350.17729750985879267No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56080.17337130519409052No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG51670.15973779135839264No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47810.14780460237748697No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG43210.13358370359195174No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA42460.13126507878996227No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41000.1267514891754228No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40250.12443286437343339No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37450.1157766651126728No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35960.11117033050605377No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG34950.10804791577270799No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG34420.10640942091263544No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34150.10557471598391925No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA32980.10195766129281573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61950.059.2358741
GTATCAA113800.043.4827771
TATCAAC151050.032.696682
ATCAACG153250.032.176363
TCAACGC155250.031.7618524
CAACGCA157750.031.2584935
AACGCAG164000.030.0528346
TAAGGTG26200.028.8294185
TGGTATC25100.028.7858242
AGGTAAG23900.027.844472
GTAAGGT26850.027.600724
AAGGTAA24850.026.0148491
GGTAAGG28250.025.8965743
ACGCAGA190750.025.7636687
CGCAGAG190850.025.5760128
TACATGG139900.024.1240062
GTACATG144150.023.8084621
ACATGGG141150.022.8185943
GCAGAGT212650.022.4405029
GTGGTAT35350.022.321711
Produced by FastQC (version 0.11.5)