##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938615_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3234676 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847143268753964 31.0 31.0 34.0 30.0 34.0 2 32.06560718909714 33.0 31.0 34.0 30.0 34.0 3 32.124697496750834 33.0 31.0 34.0 30.0 34.0 4 35.796383625438835 37.0 35.0 37.0 35.0 37.0 5 35.69366267286121 37.0 35.0 37.0 33.0 37.0 6 35.698946664209956 37.0 35.0 37.0 33.0 37.0 7 35.63858729591465 37.0 35.0 37.0 33.0 37.0 8 35.60855244852962 37.0 35.0 37.0 33.0 37.0 9 37.20173797932158 39.0 37.0 39.0 33.0 39.0 10-11 37.10447027770324 39.0 37.0 39.0 33.0 39.0 12-13 37.00383917894713 39.0 37.0 39.0 33.0 39.0 14-15 38.06428062656043 40.0 37.0 41.0 33.0 41.0 16-17 37.98364457522175 40.0 37.0 41.0 33.0 41.0 18-19 38.220631216233095 40.0 37.5 41.0 33.0 41.0 20-21 38.18344773943356 40.0 37.5 41.0 33.0 41.0 22-23 38.08930724437316 40.0 37.0 41.0 33.0 41.0 24-25 37.96239777956123 40.0 37.0 41.0 33.0 41.0 26-27 37.77406763459463 40.0 37.0 41.0 32.0 41.0 28-29 37.665833301387835 40.0 37.0 41.0 32.0 41.0 30-31 37.40063270633597 39.0 36.0 41.0 31.5 41.0 32-33 37.18286468258336 39.0 36.0 41.0 31.0 41.0 34-35 37.08544286970318 39.0 36.0 41.0 31.0 41.0 36-37 37.0289075320063 39.0 36.0 41.0 31.0 41.0 38-39 36.85705338030765 39.0 35.0 41.0 30.0 41.0 40-41 36.673432980613825 39.0 35.0 41.0 30.0 41.0 42-43 36.43608788020809 39.0 35.0 41.0 30.0 41.0 44-45 36.13063379454387 39.0 35.0 40.0 30.0 41.0 46-47 35.7401674850897 38.0 35.0 40.0 28.5 41.0 48-49 35.362355766079816 38.0 34.5 40.0 27.0 41.0 50-51 34.97715675387582 38.0 34.0 40.0 26.0 41.0 52-53 34.48722221329122 38.0 33.0 40.0 24.5 41.0 54-55 34.148621840332694 37.5 33.0 40.0 24.0 41.0 56-57 33.81057994680147 37.0 33.0 40.0 23.0 41.0 58-59 33.33608481962336 36.5 32.0 39.0 22.5 41.0 60-61 32.94550597957879 36.0 31.5 39.0 22.0 40.0 62-63 32.472600346989935 35.5 31.0 39.0 20.0 40.0 64-65 31.95573173325551 35.0 31.0 38.0 18.5 40.0 66-67 31.46716286267929 35.0 30.0 38.0 17.5 40.0 68-69 30.99275970761832 34.5 30.0 37.0 13.5 39.5 70-71 30.437302994179326 34.0 29.0 37.0 9.0 39.0 72-73 29.96132518372783 34.0 29.0 36.0 7.5 39.0 74-75 29.40112796459367 34.0 28.0 36.0 7.0 38.5 76-77 28.18833926489083 31.5 26.5 34.5 3.5 36.5 78-79 28.885827359525344 33.5 28.0 35.0 2.0 37.0 80-81 28.56330247604397 33.0 27.5 35.0 2.0 36.5 82-83 28.1515023452117 33.0 27.0 35.0 2.0 36.0 84-85 27.641830742862652 33.0 26.0 35.0 2.0 36.0 86-87 27.094218400853748 32.5 25.5 35.0 2.0 35.0 88-89 26.38802587956259 32.0 24.5 34.0 2.0 35.0 90-91 25.83083885372136 32.0 23.5 34.0 2.0 35.0 92-93 25.06291016472747 31.0 20.0 34.0 2.0 35.0 94-95 24.31325795844777 31.0 16.5 34.0 2.0 35.0 96-97 23.324986335571168 31.0 3.5 34.0 2.0 35.0 98-99 22.045125075896316 30.0 2.0 34.0 2.0 35.0 100-101 18.119202355970117 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25063204189966726 1101 2 0.2546264237861635 1101 3 0.28131316326085454 1101 4 0.23240887246836195 1101 5 0.05577675131364401 1101 6 0.19407993230780107 1101 7 0.22247158281634682 1101 8 0.20427720971431995 1101 9 0.2491121582154392 1101 10-11 0.2204013958365607 1101 12-13 0.3450816857819632 1101 14-15 0.39219414338804626 1101 16-17 0.4096613444493613 1101 18-19 0.4677029543551896 1101 20-21 0.36087711970989744 1101 22-23 0.32750432347030767 1101 24-25 0.43514525532778947 1101 26-27 0.34592696445250226 1101 28-29 0.27098894076794267 1101 30-31 0.4005194438228017 1101 32-33 0.26617292826030337 1101 34-35 0.27558106015498396 1101 36-37 0.46261807358148843 1101 38-39 0.42641999394251684 1101 40-41 0.44031718200406544 1101 42-43 0.4798265398787933 1101 44-45 0.5505372382271574 1101 46-47 0.3339670784801072 1101 48-49 0.339171195356208 1101 50-51 0.4900418256166219 1101 52-53 0.47484532153401204 1101 54-55 0.4320803166088325 1101 56-57 0.3667791795644533 1101 58-59 0.4497690559287335 1101 60-61 0.4262423596821918 1101 62-63 0.5391645458957868 1101 64-65 0.46107260756933854 1101 66-67 0.2871682757217222 1101 68-69 0.37485957822294225 1101 70-71 0.31390556251538726 1101 72-73 0.24566273681522333 1101 74-75 0.26673675577303513 1101 76-77 0.24601482578604106 1101 78-79 0.37792093679799166 1101 80-81 0.3502989996117627 1101 82-83 0.3630581158265791 1101 84-85 0.4864972495887976 1101 86-87 0.5932621984836892 1101 88-89 0.6057471412420377 1101 90-91 0.5548879096419199 1101 92-93 0.7382714344377632 1101 94-95 0.5119492585322583 1101 96-97 0.5888924369388135 1101 98-99 0.6358354670645738 1101 100-101 0.7096121023448028 1102 1 0.3127331362404213 1102 2 0.2682652813622539 1102 3 0.2788356835365846 1102 4 0.24666173800510904 1102 5 0.09793779996542185 1102 6 0.13511920687061263 1102 7 0.2390273522965103 1102 8 0.28460102284840616 1102 9 0.22815308875423312 1102 10-11 0.3482502231860991 1102 12-13 0.3629767008952385 1102 14-15 0.5648543291366721 1102 16-17 0.5291484906840154 1102 18-19 0.3751738001997893 1102 20-21 0.36565525883183625 1102 22-23 0.3712926677936039 1102 24-25 0.4476536100258741 1102 26-27 0.3732001905131739 1102 28-29 0.4112603312889789 1102 30-31 0.4486123281139811 1102 32-33 0.4765182890336348 1102 34-35 0.514629921422106 1102 36-37 0.4724678885311633 1102 38-39 0.5737188078247826 1102 40-41 0.5189608279444329 1102 42-43 0.5315451137442935 1102 44-45 0.5249623851202045 1102 46-47 0.5935081436916363 1102 48-49 0.4450197334087349 1102 50-51 0.6037754529023189 1102 52-53 0.5047745934033756 1102 54-55 0.5349675506301566 1102 56-57 0.5792007451600583 1102 58-59 0.5956760020496645 1102 60-61 0.7086762952188366 1102 62-63 0.5774729564581165 1102 64-65 0.5356369162991221 1102 66-67 0.4756722111586136 1102 68-69 0.7030415959199381 1102 70-71 0.6919197380687905 1102 72-73 0.7543750658556512 1102 74-75 0.6878464591937252 1102 76-77 0.6038662468328972 1102 78-79 0.9613517000433056 1102 80-81 0.9432202010921635 1102 82-83 0.8373633042187869 1102 84-85 0.8123686321292212 1102 86-87 0.7437155967259628 1102 88-89 0.8057266909362824 1102 90-91 0.8228185880932841 1102 92-93 1.1438117215401036 1102 94-95 1.0042043287117224 1102 96-97 1.1469121989172102 1102 98-99 1.2225557368090918 1102 100-101 1.3810430252022599 1103 1 0.2008071603550512 1103 2 0.21935978753290186 1103 3 0.20282075092266894 1103 4 0.14508860724415484 1103 5 0.07595242493436416 1103 6 0.1032122813088634 1103 7 0.1939833945622027 1103 8 0.15393059344701499 1103 9 0.2088572407781797 1103 10-11 0.23066121321130595 1103 12-13 0.3562017076989292 1103 14-15 0.46225413745019495 1103 16-17 0.414149614771425 1103 18-19 0.4587795768258758 1103 20-21 0.3385964039811 1103 22-23 0.3640763223792902 1103 24-25 0.46104178699273035 1103 26-27 0.4494573935470214 1103 28-29 0.43045294071000484 1103 30-31 0.356217371125112 1103 32-33 0.48911073043318254 1103 34-35 0.35709025296872454 1103 36-37 0.446242929057469 1103 38-39 0.4660318247570814 1103 40-41 0.48145535541357987 1103 42-43 0.6795587329508166 1103 44-45 0.6808082313213788 1103 46-47 0.6095828895887152 1103 48-49 0.7123537530270241 1103 50-51 0.7258951267986404 1103 52-53 0.6576907128169509 1103 54-55 0.535249164109743 1103 56-57 0.6076957695941658 1103 58-59 0.6247108842571336 1103 60-61 0.6690113915945375 1103 62-63 0.7117948950947763 1103 64-65 0.8784906784422297 1103 66-67 0.7964341057783777 1103 68-69 0.6140467075595701 1103 70-71 0.4774340968064976 1103 72-73 0.4221161234444253 1103 74-75 0.48282592980771355 1103 76-77 0.4818240758059531 1103 78-79 0.6858760335777916 1103 80-81 0.7745372765716745 1103 82-83 0.6881483905368668 1103 84-85 0.5591061163032514 1103 86-87 0.42319212696391517 1103 88-89 0.31425673956785616 1103 90-91 0.5868980788225215 1103 92-93 0.9121784225210199 1103 94-95 0.7736471954896125 1103 96-97 0.9699651611833708 1103 98-99 0.9851768720190606 1103 100-101 1.2156258704450025 1104 1 0.2310512703480292 1104 2 0.21761232807772046 1104 3 0.26251934879919503 1104 4 0.18629309430313157 1104 5 0.12154930866464753 1104 6 0.08273685887422744 1104 7 0.15098362214752115 1104 8 0.23825656159166186 1104 9 0.2773983529117743 1104 10-11 0.32008030992846415 1104 12-13 0.2778722828960838 1104 14-15 0.358373642831431 1104 16-17 0.39736734135900065 1104 18-19 0.3108087143434801 1104 20-21 0.33236452422531215 1104 22-23 0.43415577934092653 1104 24-25 0.4360911796037925 1104 26-27 0.35871582822178993 1104 28-29 0.4446712511034079 1104 30-31 0.368242616603915 1104 32-33 0.48036023980540676 1104 34-35 0.4387160367362881 1104 36-37 0.4812625376766917 1104 38-39 0.571552747763981 1104 40-41 0.5132269257193016 1104 42-43 0.6059505491146453 1104 44-45 0.6029977999977234 1104 46-47 0.6318864158418407 1104 48-49 0.7533672663176745 1104 50-51 0.8268689610835409 1104 52-53 0.6328431784895017 1104 54-55 0.7081846519174348 1104 56-57 0.7177552056443446 1104 58-59 0.8083653107916007 1104 60-61 0.8972087179617887 1104 62-63 0.8429216403278588 1104 64-65 0.8250472136041616 1104 66-67 0.8281690645326556 1104 68-69 0.7315183336072018 1104 70-71 0.9249008083305625 1104 72-73 0.7990678324782436 1104 74-75 0.8146250412354625 1104 76-77 0.7180437846946468 1104 78-79 0.9306202969434842 1104 80-81 0.9322908445487705 1104 82-83 1.0088596441361766 1104 84-85 1.0632913773502146 1104 86-87 1.1518188969345253 1104 88-89 1.307768249118194 1104 90-91 1.2310702454677482 1104 92-93 1.211668994221938 1104 94-95 1.3347108105339913 1104 96-97 1.3400040485880034 1104 98-99 1.4558802096072512 1104 100-101 1.762372060040068 1105 1 0.17572504629115215 1105 2 0.22002215826144322 1105 3 0.21111210414317583 1105 4 0.1630541691034182 1105 5 0.11612426245928731 1105 6 0.17078597526982975 1105 7 0.11051216097297356 1105 8 0.1445610817648202 1105 9 0.19413129144970043 1105 10-11 0.1784289628525073 1105 12-13 0.26646026666533373 1105 14-15 0.4323151731629835 1105 16-17 0.29857940764813407 1105 18-19 0.3353909549615395 1105 20-21 0.26473115131379643 1105 22-23 0.2574142470366212 1105 24-25 0.2616229266042964 1105 26-27 0.08133691463660853 1105 28-29 0.06798717497027695 1105 30-31 0.27519382694405436 1105 32-33 0.351135022711091 1105 34-35 0.3754858902477096 1105 36-37 0.4238430472118253 1105 38-39 0.32470605587084833 1105 40-41 0.3887825305934598 1105 42-43 0.4808804242276139 1105 44-45 0.41415440751905663 1105 46-47 0.39442782805534904 1105 48-49 0.5944639640673941 1105 50-51 0.6082174056364877 1105 52-53 0.598252652346325 1105 54-55 0.5513040288262161 1105 56-57 0.39282052196768547 1105 58-59 0.46169191213578387 1105 60-61 0.5200203591245725 1105 62-63 0.43473931438861513 1105 64-65 0.5303941514738177 1105 66-67 0.5440886776775464 1105 68-69 0.5524992066204355 1105 70-71 0.6999189020926195 1105 72-73 0.684074114435159 1105 74-75 0.7189697634669336 1105 76-77 0.650533400046541 1105 78-79 0.960503670779719 1105 80-81 0.7574789025425162 1105 82-83 0.7949987743779765 1105 84-85 0.8649319606413179 1105 86-87 0.8434780733385345 1105 88-89 0.9340941489052597 1105 90-91 1.077646239884885 1105 92-93 1.4261700810806275 1105 94-95 1.606188445164527 1105 96-97 1.6850582558962834 1105 98-99 1.576894187731984 1105 100-101 1.433310334110935 1106 1 0.054141680718178264 1106 2 0.08771987937932835 1106 3 0.18350268893957633 1106 4 0.009252654761382928 1106 5 0.05275033788162631 1106 6 -0.019725744607598017 1106 7 0.08249437697243422 1106 8 0.10204159980980876 1106 9 -0.0801113840107135 1106 10-11 0.20366799676860836 1106 12-13 0.046733290375286174 1106 14-15 0.10659653619363496 1106 16-17 0.10489438587870126 1106 18-19 0.07346228747199035 1106 20-21 0.25137626347704867 1106 22-23 0.15648728057199435 1106 24-25 0.23116414114836914 1106 26-27 0.20685351651695072 1106 28-29 0.2065009581847832 1106 30-31 0.22350139926831503 1106 32-33 0.17363583637823865 1106 34-35 0.10673470045140476 1106 36-37 0.13039475652693255 1106 38-39 -0.016315664436440613 1106 40-41 0.052547629492394776 1106 42-43 0.1665546626044616 1106 44-45 0.16284179352889083 1106 46-47 -0.5350303003259782 1106 48-49 -0.2174468948580781 1106 50-51 0.17791490568254886 1106 52-53 0.17437505244949136 1106 54-55 0.20583016749966987 1106 56-57 0.22843519376813504 1106 58-59 0.21752728731309645 1106 60-61 0.315059138808067 1106 62-63 0.015052867934052472 1106 64-65 0.18564184403659212 1106 66-67 0.2756261094369705 1106 68-69 0.09200658489275781 1106 70-71 0.19837600494629015 1106 72-73 0.5662290120973203 1106 74-75 0.5757351755509639 1106 76-77 0.42374747740939966 1106 78-79 0.6396209800899122 1106 80-81 0.039331668703788125 1106 82-83 0.49199526793311676 1106 84-85 0.6590311850476489 1106 86-87 0.7892233617451581 1106 88-89 0.6303782998973197 1106 90-91 0.8382147015319887 1106 92-93 0.9261393766598012 1106 94-95 1.0886961366219445 1106 96-97 1.3165545437318045 1106 98-99 1.136583581810946 1106 100-101 1.1791590964157344 1107 1 0.16603515780966305 1107 2 0.19402459511739067 1107 3 0.17599708163744054 1107 4 0.0850993963801372 1107 5 0.09936111568936212 1107 6 0.17131463983013617 1107 7 0.17148779373398781 1107 8 0.13369879355369108 1107 9 0.19145231562339404 1107 10-11 0.21228264170703426 1107 12-13 0.200997917674286 1107 14-15 0.3292130393200168 1107 16-17 0.3815977116092242 1107 18-19 0.31812088443079745 1107 20-21 0.3702442502162313 1107 22-23 0.15992694583289335 1107 24-25 0.22962763808549624 1107 26-27 0.2351561364461503 1107 28-29 0.261963021083524 1107 30-31 0.20307931206947671 1107 32-33 0.2848324696338267 1107 34-35 0.33310779010874114 1107 36-37 0.3295340999815082 1107 38-39 0.3972310992911403 1107 40-41 0.37073681813378556 1107 42-43 0.24361430759845604 1107 44-45 0.33543083107925753 1107 46-47 0.3882165146526191 1107 48-49 0.45953528049214043 1107 50-51 0.5143575639019602 1107 52-53 0.4021233824014345 1107 54-55 0.22215030524506574 1107 56-57 0.2783247943884888 1107 58-59 0.3369507502659559 1107 60-61 0.41889989208126366 1107 62-63 0.5009608464387085 1107 64-65 0.30157473630792353 1107 66-67 0.27781401507494863 1107 68-69 0.40549159894405307 1107 70-71 0.5129899517799217 1107 72-73 0.5577850170735381 1107 74-75 0.3059936564640928 1107 76-77 0.45042042334532084 1107 78-79 0.700659521658018 1107 80-81 0.7035077215049448 1107 82-83 0.6918737207333798 1107 84-85 0.8608165829258176 1107 86-87 1.0465178049561423 1107 88-89 1.114283687175952 1107 90-91 1.3469194350542573 1107 92-93 1.4233057661176538 1107 94-95 1.4107119651254152 1107 96-97 1.4792552733792803 1107 98-99 1.6411876268397307 1107 100-101 1.8560137747032819 1108 1 0.11588155732605898 1108 2 -0.019061069417013243 1108 3 0.03417294716981445 1108 4 0.03820584412320471 1108 5 0.04626951309533922 1108 6 0.09121012641444537 1108 7 0.026657360554324327 1108 8 0.04073793526334413 1108 9 0.10722058329258033 1108 10-11 0.10008523421393534 1108 12-13 0.10647524271216469 1108 14-15 0.17205587198844086 1108 16-17 0.2041984034020814 1108 18-19 0.18716879324859548 1108 20-21 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-0.8726434748028211 2316 60-61 -0.8596079313880978 2316 62-63 -1.0362199145250912 2316 64-65 -1.0105046301483078 2316 66-67 -1.1029181890096602 2316 68-69 -0.9903217766239116 2316 70-71 -0.9078207648644252 2316 72-73 -0.956590389794993 2316 74-75 -0.9714363431428659 2316 76-77 -1.0141076350079246 2316 78-79 -1.3177164724164037 2316 80-81 -1.1043210162935608 2316 82-83 -1.22264357648654 2316 84-85 -1.3555891620521265 2316 86-87 -1.3409352609557423 2316 88-89 -1.3787419884852383 2316 90-91 -1.34505306357903 2316 92-93 -1.515814804113699 2316 94-95 -1.5152343322958899 2316 96-97 -1.6609962003562195 2316 98-99 -1.2704482258354517 2316 100-101 -1.7371749790723356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 48.0 5 84.0 6 261.0 7 630.0 8 1466.0 9 2724.0 10 4468.0 11 6953.0 12 9824.0 13 13362.0 14 17359.0 15 23052.0 16 29087.0 17 35974.0 18 33311.0 19 28835.0 20 27840.0 21 28605.0 22 31329.0 23 35344.0 24 41008.0 25 47994.0 26 57463.0 27 69127.0 28 82963.0 29 98435.0 30 117935.0 31 140867.0 32 169437.0 33 210105.0 34 269587.0 35 357392.0 36 469679.0 37 516712.0 38 244670.0 39 10729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95709573813935 18.758283310152173 14.960488977690748 23.324131974017728 2 16.151148644698548 20.23800943859331 38.64397783164759 24.96686408506055 3 19.487639565755583 23.649602000323988 29.765732333006454 27.097026100913972 4 13.550476152789336 16.567594405127437 35.45069119751097 34.431238244572256 5 13.863085977972764 37.45644502314994 33.671391501483015 15.00907749739428 6 33.39335995320706 35.54770245922621 16.460752174251763 14.598185413314965 7 29.581819013712657 32.69254787805641 19.480127221397137 18.245505886833797 8 27.640171689529335 34.08236249936624 19.09486452429857 19.182601286805852 9 26.979549110946504 16.241595757967723 19.34462678796887 37.4342283431169 10-11 25.493727965335633 26.25147000812446 26.526412537144367 21.72838948939554 12-13 26.586712239494776 23.474159390306788 26.953889663137826 22.985238707060613 14-15 22.906652783771854 24.855426014846618 25.36297298400211 26.874948217379423 16-17 22.544576334693183 28.134935307276525 26.422275368537683 22.89821298949261 18-19 22.70491696850009 26.25405141040401 28.867698032198586 22.17333358889731 20-21 22.930751024213862 25.383361424760935 29.358458157787677 22.32742939323753 22-23 22.909574251022356 25.398401570976503 29.237302283134387 22.45472189486675 24-25 22.85490577996259 25.64544203805902 29.158635935012136 22.34101624696625 26-27 22.00493166252861 26.20514566057289 28.921780035855953 22.86814264104255 28-29 22.387997179313025 26.57752421960427 28.70172657328886 22.332752027793845 30-31 22.295212167379376 26.005513482898746 28.519567701390216 23.17970664833167 32-33 21.843115414287944 26.038357301043124 28.62734295937028 23.49118432529865 34-35 23.017380554006607 26.141619661033822 28.36355468172084 22.477445103238733 36-37 23.262075038053986 25.400334117476074 28.354235646361158 22.983355198108782 38-39 22.433364564124865 25.851072562046657 28.37920539405906 23.33635747976942 40-41 23.083995552521046 25.708675907360984 28.474871171967585 22.73245736815038 42-43 22.68696961776076 25.83349010004679 28.182826725661446 23.296713556531003 44-45 22.51180405249667 26.14718787376794 28.587658239540144 22.753349834195248 46-47 23.26681250301421 26.55030983010354 27.45565552778702 22.72722213909523 48-49 22.780519594543627 26.648542234214496 27.329012241102358 23.241925930139526 50-51 23.20444004417785 26.72379799684819 27.369493322698496 22.70226863627546 52-53 23.674890468164353 26.15300574153331 27.45667572269989 22.715428067602442 54-55 22.71254077564895 26.753231175032745 27.68431412587659 22.849913923441715 56-57 22.879030233630818 26.809995807926356 27.35583100131203 22.955142957130793 58-59 22.721365853145677 26.25658047212573 27.309734313510397 23.712319361218192 60-61 22.119371461005677 26.6687142699918 27.79748265359498 23.41443161540754 62-63 22.455674951187955 26.731579346049973 27.923799099972328 22.888946602789744 64-65 22.149842370783002 26.759770352349843 27.5938632824723 23.496523994394856 66-67 22.005274475219085 26.789804742390118 27.581841707000738 23.62307907539006 68-69 22.872315014952292 26.292751396477463 27.669973233827633 23.16496035474261 70-71 22.56306199635574 26.451707343614846 27.169538568647106 23.815692091382314 72-73 22.402980578107627 26.68246835964484 27.164649797066136 23.749901265181396 74-75 22.40254353967176 27.090911536577067 27.061195265124955 23.445349658626213 76-77 22.579374524156496 26.465242294911544 27.394991980543598 23.560391200388363 78-79 22.59982190522445 26.746470691286977 27.340300336629742 23.313407066858833 80-81 22.81679583351114 26.718086017634967 26.992066923083108 23.47305122577078 82-83 22.626172283747678 26.861972939108586 27.135253892432793 23.37660088471094 84-85 22.436223282977355 26.99438867543923 27.083623206419936 23.485764835163476 86-87 22.749924559476813 27.04859668259575 26.5326638914448 23.66881486648264 88-89 22.47096067440327 27.126875433077856 26.41410218309143 23.98806170942744 90-91 22.257103262567803 26.97233652102132 26.709476340651083 24.061083875759795 92-93 22.39320925293557 26.567525420772814 26.92944461915766 24.10982070713396 94-95 22.090006769170063 26.27245090032436 27.18537661355337 24.45216571695221 96-97 22.366950189775693 25.82135492655228 27.202523023205288 24.609171860466734 98-99 21.946616330296088 26.260521649562662 26.790615597212113 25.002246422929137 100-101 21.982499067380648 25.900582545045165 27.32770272933972 24.78921565823447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 509.0 1 360.5 2 347.0 3 505.5 4 461.5 5 458.0 6 809.0 7 1642.5 8 2567.0 9 2968.5 10 2611.0 11 2146.0 12 2202.0 13 2412.5 14 2609.0 15 2890.0 16 3172.0 17 3442.0 18 3824.0 19 4336.5 20 4914.5 21 5702.5 22 6863.0 23 8336.0 24 10084.5 25 12693.0 26 16210.0 27 20279.5 28 24209.5 29 28044.5 30 32555.5 31 41045.0 32 55595.5 33 59367.0 34 54152.5 35 56019.5 36 62221.0 37 69025.0 38 74886.0 39 79396.0 40 84478.5 41 91668.5 42 95447.5 43 99470.5 44 104572.0 45 111819.5 46 117321.0 47 121700.0 48 122773.0 49 119607.5 50 120179.5 51 118179.0 52 115594.0 53 118883.0 54 125353.0 55 119615.5 56 107946.5 57 99036.5 58 92761.5 59 83205.0 60 65902.5 61 55110.0 62 48659.0 63 35926.5 64 24353.0 65 18377.0 66 13384.0 67 10353.0 68 8304.0 69 6520.0 70 5004.0 71 3241.0 72 2206.0 73 1776.0 74 1321.5 75 962.5 76 710.5 77 477.5 78 308.0 79 213.0 80 131.5 81 82.0 82 56.5 83 31.5 84 13.5 85 8.0 86 5.0 87 3.5 88 3.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.78234976238733E-4 2 0.05499778030318957 3 0.0 4 0.0 5 0.04346648628796207 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006986789403328185 26-27 0.002071304823110568 28-29 9.274499207957767E-5 30-31 0.0018548998415915535 32-33 3.555224696383811E-4 34-35 0.012381456442623619 36-37 0.024221900431449703 38-39 0.00477636709209825 40-41 0.0010665674089151433 42-43 0.0012211423957144394 44-45 0.009259041709277839 46-47 0.0 48-49 0.0 50-51 7.265024379566918E-4 52-53 0.0 54-55 0.001715782353472187 56-57 0.0 58-59 0.023820005465771534 60-61 0.0 62-63 0.010232864126113404 64-65 0.01558115866936905 66-67 0.007141364390127481 68-69 0.01720419603076166 70-71 6.182999471971845E-5 72-73 4.637249603978884E-5 74-75 0.02341811050009336 76-77 0.04951036827181455 78-79 0.031054114847978594 80-81 0.027019707692516964 82-83 0.036804304356912405 84-85 0.01551932867464933 86-87 0.010511099102352137 88-89 0.08416608031221674 90-91 0.025582160315283507 92-93 0.024886572874686676 94-95 0.02773075263179373 96-97 0.03875194919058354 98-99 0.05436402285731245 100-101 0.01562753116540884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3234676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.719087529959126 #Duplication Level Percentage of deduplicated Percentage of total 1 68.98048400506907 28.088223660613533 2 15.159963129248787 12.345997312216687 3 5.961421941182475 7.28230985478084 4 3.0467742266270257 4.962474656721976 5 1.7893472145202538 3.6430292924769376 6 1.0702070221406934 2.614671204583428 7 0.7495569867706405 2.136489358710429 8 0.5172887797061355 1.68508216792959 9 0.3879278881709268 1.4216462670369747 >10 2.068018845780706 15.27131404063219 >50 0.16260955082543155 4.561100343546016 >100 0.09225535134501939 7.200839675316417 >500 0.008289308541929566 2.3345212269828943 >1k 0.005171311750775036 4.573170672581766 >5k 6.083896177382396E-4 1.5565229605322413 >10k+ 7.604870221727994E-5 0.3226073053382053 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10418 0.3220724424950134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8766 0.27100086685652597 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6538 0.20212225273875964 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6500 0.20094748283908498 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6051 0.18706664902450817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5900 0.18239848442316944 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5735 0.17729750985879267 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5608 0.17337130519409052 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5167 0.15973779135839264 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4781 0.14780460237748697 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4321 0.13358370359195174 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4246 0.13126507878996227 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4100 0.1267514891754228 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4025 0.12443286437343339 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3745 0.1157766651126728 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3596 0.11117033050605377 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3495 0.10804791577270799 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3442 0.10640942091263544 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3415 0.10557471598391925 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3298 0.10195766129281573 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.274499207957767E-5 0.0 20-21 0.0 0.0 0.0 1.391174881193665E-4 0.0 22-23 0.0 0.0 0.0 3.864374669982403E-4 0.0 24-25 0.0 0.0 0.0 7.110449392767621E-4 0.0 26-27 0.0 0.0 0.0 0.0014066323798735947 0.0 28-29 0.0 0.0 0.0 0.0049927720736172645 0.0 30-31 0.0 0.0 0.0 0.016153086120526447 0.0 32-33 0.0 0.0 0.0 0.03924658914834129 0.0 34-35 0.0 0.0 0.0 0.07373226870326424 0.0 36-37 0.0 0.0 0.0 0.13097138631504363 0.0 38-39 0.0 0.0 0.0 0.22196968104378922 0.0 40-41 0.0 0.0 0.0 0.31896548526034757 0.0 42-43 0.0 0.0 0.0 0.4179553068066168 0.0 44-45 0.0 0.0 0.0 0.5410897412909361 0.0 46-47 0.0 0.0 0.0 0.6903318910456565 0.0 48-49 0.0 0.0 0.0 0.8463135102248263 0.0 50-51 0.0 0.0 0.0 1.0173043606222076 0.0 52-53 0.0 0.0 0.0 1.1825604790093351 0.0 54-55 0.0 0.0 0.0 1.3634441285618715 0.0 56-57 0.0 0.0 0.0 1.5680550385880996 0.0 58-59 0.0 0.0 0.0 1.7870877948827024 0.0 60-61 0.0 0.0 0.0 2.0361080986163684 0.0 62-63 0.0 0.0 0.0 2.2795946178226196 0.0 64-65 0.0 0.0 0.0 2.526713649218654 0.0 66-67 0.0 0.0 0.0 2.8209935090871543 0.0 68-69 0.0 0.0 0.0 3.1487852260937417 0.0 70-71 0.0 0.0 0.0 3.48510948237165 0.0 72-73 0.0 0.0 0.0 3.8480051788803578 0.0 74-75 0.0 0.0 0.0 4.204486013436894 0.0 76-77 0.0 0.0 0.0 4.585466983401119 0.0 78-79 0.0 0.0 0.0 5.014489859262566 0.0 80-81 0.0 0.0 0.0 5.468723915470978 0.0 82-83 0.0 0.0 0.0 5.940100337715432 0.0 84-85 0.0 0.0 0.0 6.422235179041116 0.0 86-87 0.0 0.0 0.0 6.9114186397648485 0.0 88-89 0.0 0.0 0.0 7.430064092972526 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6195 0.0 59.235874 1 GTATCAA 11380 0.0 43.482777 1 TATCAAC 15105 0.0 32.69668 2 ATCAACG 15325 0.0 32.17636 3 TCAACGC 15525 0.0 31.761852 4 CAACGCA 15775 0.0 31.258493 5 AACGCAG 16400 0.0 30.052834 6 TAAGGTG 2620 0.0 28.829418 5 TGGTATC 2510 0.0 28.785824 2 AGGTAAG 2390 0.0 27.84447 2 GTAAGGT 2685 0.0 27.60072 4 AAGGTAA 2485 0.0 26.014849 1 GGTAAGG 2825 0.0 25.896574 3 ACGCAGA 19075 0.0 25.763668 7 CGCAGAG 19085 0.0 25.576012 8 TACATGG 13990 0.0 24.124006 2 GTACATG 14415 0.0 23.808462 1 ACATGGG 14115 0.0 22.818594 3 GCAGAGT 21265 0.0 22.440502 9 GTGGTAT 3535 0.0 22.32171 1 >>END_MODULE