FastQCFastQC Report
Thu 26 May 2016
SRR938614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243225
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110190.3397544111185625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85360.263194813804161No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67040.20670782939820703No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65270.20125029869959687No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61540.18974940067371213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58810.18133185332500829No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA54000.1665009365677682No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52400.16156757548427877No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA47620.1468291592473541No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46780.14423914467852217No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46400.1430674714211934No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43080.13283074717295285No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41860.12906905934679216No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40110.12367319566172559No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35100.10822560876904933No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33820.10427891990225778No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33650.10375475028713704No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33270.10258307702980829No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG32880.10138057026570775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.058.7751921
GTATCAA109350.046.0504071
TATCAAC147600.033.8702622
TGGTATC23900.033.801832
ATCAACG148900.033.6383823
TCAACGC152950.032.747664
CAACGCA153850.032.555095
TAAGGTG23000.032.4375155
AACGCAG159700.031.4772116
AGGTAAG21800.030.082352
AAGGTAA23350.028.1292921
GTAAGGT25200.028.0979674
GGTAAGG24800.027.9763033
GTGGTAT30550.027.5758321
ACGCAGA183750.027.1979967
CGCAGAG184850.026.9337648
TACATGG142300.024.4119052
GTACATG147150.024.1940141
GCAGAGT200800.024.1071349
ACATGGG144800.023.0386873
Produced by FastQC (version 0.11.5)