FastQCFastQC Report
Thu 26 May 2016
SRR938613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240411
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113260.34952356352326913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85470.26376283749191076No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66350.20475797668875953No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65810.20309152141503037No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61970.1912411728018452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59470.18352610209013612No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55970.17272500309374336No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52140.160905514763405No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA48850.15075248170679584No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47400.14627774069400457No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46980.14498160881443742No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43720.13492115660636875No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41910.12933544541109135No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38700.11942929461725689No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35330.10902937929787301No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35270.108844217600792No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33040.10196237452594749No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG32720.10097484547484871No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT32710.10094398519200187No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32490.10026505896937148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.059.5725481
GTATCAA109850.045.2935751
TATCAAC143900.034.5119132
ATCAACG145900.033.843913
TCAACGC148800.033.216264
CAACGCA150350.032.873825
AACGCAG155100.031.8932576
TGGTATC22900.030.9217742
CGCAGAG179450.027.1364578
ACGCAGA180250.027.0950877
TACATGG136750.025.5778522
GTACATG142200.025.169611
TAAGGTG23550.025.0236745
GTAAGGT24700.024.4358274
GCAGAGT199150.023.8784649
GTGGTAT31500.023.753371
ACATGGG141650.023.720463
AGGTAAG23100.023.2477022
TAAGGTA13100.023.2182224
GTGTAAG14000.023.1481881
Produced by FastQC (version 0.11.5)