##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3240411 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.249668329110104 31.0 31.0 34.0 28.0 34.0 2 31.380478278835618 31.0 31.0 34.0 28.0 34.0 3 31.439295817721888 31.0 31.0 34.0 28.0 34.0 4 35.1159717085271 37.0 35.0 37.0 32.0 37.0 5 35.12930643674522 37.0 35.0 37.0 32.0 37.0 6 35.14610615752137 37.0 35.0 37.0 32.0 37.0 7 35.06817437664543 37.0 35.0 37.0 32.0 37.0 8 35.01803042885609 37.0 35.0 37.0 32.0 37.0 9 36.52520313009677 39.0 37.0 39.0 32.0 39.0 10-11 36.391815883849304 39.0 36.0 39.0 32.0 39.0 12-13 36.24279296669466 39.0 35.0 39.0 32.0 39.0 14-15 37.26406434245533 39.5 36.5 41.0 31.5 41.0 16-17 37.10951187982019 39.0 36.0 41.0 31.0 41.0 18-19 36.999905104630244 39.0 36.0 41.0 31.0 41.0 20-21 36.97482310114365 39.0 36.0 41.0 31.0 41.0 22-23 37.077629967309704 39.0 36.0 41.0 31.0 41.0 24-25 36.99747269713626 39.0 36.0 41.0 30.5 41.0 26-27 36.81173946761692 39.0 36.0 41.0 30.0 41.0 28-29 36.63382607946954 39.0 36.0 41.0 30.0 41.0 30-31 36.35091412786835 39.0 35.5 40.0 30.0 41.0 32-33 36.03796910330202 38.5 35.0 40.0 29.0 41.0 34-35 35.78218920377692 38.0 35.0 40.0 27.5 41.0 36-37 35.48992365474626 38.0 34.5 40.0 27.0 41.0 38-39 35.15497339689317 38.0 34.0 40.0 26.0 41.0 40-41 35.03498753707477 38.0 34.0 40.0 25.0 41.0 42-43 34.785346365013574 38.0 33.5 40.0 24.5 41.0 44-45 34.47726569253098 38.0 33.0 40.0 24.0 41.0 46-47 34.07365238545357 38.0 33.0 40.0 23.0 41.0 48-49 33.65713222797972 38.0 33.0 40.0 21.0 41.0 50-51 32.849159875089924 36.5 31.5 39.5 19.0 40.5 52-53 32.8413958908299 37.0 31.5 39.0 19.0 40.0 54-55 32.907101599149 37.0 32.0 40.0 17.5 41.0 56-57 32.57933222051153 36.5 31.0 40.0 14.0 41.0 58-59 32.149267947800446 36.0 31.0 39.0 10.5 41.0 60-61 31.665461726922913 35.5 30.5 39.0 8.0 40.5 62-63 31.089265219751447 35.0 29.5 39.0 6.0 40.0 64-65 30.589167392654822 35.0 29.0 38.0 2.0 40.0 66-67 30.049433081173962 34.5 28.5 38.0 2.0 40.0 68-69 29.448100565020916 34.0 28.0 37.0 2.0 39.5 70-71 28.811318533358886 34.0 26.5 36.5 2.0 39.0 72-73 28.191048913239708 34.0 26.0 36.0 2.0 39.0 74-75 27.525708467228384 33.0 25.5 35.5 2.0 38.5 76-77 26.812422097073487 32.5 24.0 35.0 2.0 37.0 78-79 26.047167627810175 32.0 21.5 35.0 2.0 37.0 80-81 25.334175510452226 31.5 19.0 35.0 2.0 36.0 82-83 24.622944126532097 31.0 15.5 34.5 2.0 36.0 84-85 23.917666308378784 31.0 7.5 34.0 2.0 35.0 86-87 23.222445239199594 30.0 3.5 34.0 2.0 35.0 88-89 22.45169779389096 29.5 2.0 34.0 2.0 35.0 90-91 21.638934999294843 29.0 2.0 34.0 2.0 35.0 92-93 20.795668203817357 29.0 2.0 34.0 2.0 35.0 94-95 19.894278997324722 27.0 2.0 34.0 2.0 35.0 96-97 18.83331713168484 25.5 2.0 33.5 2.0 35.0 98-99 17.56986166261008 22.0 2.0 33.0 2.0 35.0 100-101 14.69302474284898 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6125240733769282 1101 2 0.6515767287805829 1101 3 0.553512688942142 1101 4 0.45177234029265634 1101 5 0.613415060862863 1101 6 0.49071899688843246 1101 7 0.5624087739167436 1101 8 0.46885672362333963 1101 9 0.6717193644880979 1101 10-11 0.6701468818035465 1101 12-13 0.7011307139092722 1101 14-15 0.760324207311065 1101 16-17 0.9189519114314564 1101 18-19 0.9227972187937112 1101 20-21 0.9969329248933718 1101 22-23 0.8708147748436019 1101 24-25 0.8477625173980528 1101 26-27 0.9280372433610822 1101 28-29 1.0035943272524221 1101 30-31 0.9439117693182411 1101 32-33 1.0120975403683588 1101 34-35 1.1177258012392386 1101 36-37 1.0317480517318316 1101 38-39 1.174467906557588 1101 40-41 1.2574756120060329 1101 42-43 1.324754151915407 1101 44-45 1.2982192119170648 1101 46-47 1.4954480665986765 1101 48-49 1.4017008458188798 1101 50-51 1.2698745903616313 1101 52-53 1.468405512871584 1101 54-55 1.5259925569998032 1101 56-57 1.6728423287715017 1101 58-59 1.793334533192116 1101 60-61 1.807292635665931 1101 62-63 1.8076601810669857 1101 64-65 1.8747657258185022 1101 66-67 2.063567935660526 1101 68-69 2.066692798119554 1101 70-71 2.203390231275961 1101 72-73 2.372266552492853 1101 74-75 2.522897208801279 1101 76-77 2.8289320255674646 1101 78-79 3.1011641741420455 1101 80-81 3.6154352389807727 1101 82-83 3.739632862836146 1101 84-85 3.7945307656652396 1101 86-87 4.019366094039153 1101 88-89 4.13437361547048 1101 90-91 4.392270250708037 1101 92-93 4.458579378579163 1101 94-95 4.80761512161029 1101 96-97 5.017549627653175 1101 98-99 5.351289461837304 1101 100-101 5.255484452263024 1102 1 0.5600364695806412 1102 2 0.4530396336917626 1102 3 0.47207555919353794 1102 4 0.4316118570186376 1102 5 0.36426543311510073 1102 6 0.3972853171885049 1102 7 0.4833275587740431 1102 8 0.43295057584111873 1102 9 0.6660526569696472 1102 10-11 0.609269310999565 1102 12-13 0.5999036573881327 1102 14-15 0.8552252753411054 1102 16-17 0.870688805909758 1102 18-19 0.8886362000787145 1102 20-21 0.9025549652266704 1102 22-23 0.7329646089057107 1102 24-25 0.5794072846829224 1102 26-27 0.6021862095155086 1102 28-29 0.6488967961645997 1102 30-31 0.6752897153676756 1102 32-33 0.6689425006586305 1102 34-35 0.8514929984909188 1102 36-37 0.8940769656216645 1102 38-39 0.8878713251067083 1102 40-41 1.2433935014641904 1102 42-43 1.3643744076167295 1102 44-45 1.2797218972152251 1102 46-47 1.2891309550201342 1102 48-49 1.2953729046639495 1102 50-51 1.4240187724896671 1102 52-53 1.3873298828613017 1102 54-55 1.5154116240941065 1102 56-57 1.5903546144955456 1102 58-59 1.682850436996695 1102 60-61 1.6632235565858764 1102 62-63 1.8401579669774435 1102 64-65 1.8562185668030686 1102 66-67 2.0967433729994163 1102 68-69 2.2153167225165227 1102 70-71 2.289831832881667 1102 72-73 2.3778448762799727 1102 74-75 2.3973370062483745 1102 76-77 2.474543709074574 1102 78-79 2.8606225180416054 1102 80-81 3.45471995766486 1102 82-83 3.5306599357493837 1102 84-85 3.6405218568934394 1102 86-87 3.7500258580391765 1102 88-89 4.220733320813508 1102 90-91 4.454010719075011 1102 92-93 4.308157461880313 1102 94-95 4.497560752769054 1102 96-97 4.575574767686149 1102 98-99 4.7801995234258605 1102 100-101 5.123822046771311 1103 1 0.4462482434673909 1103 2 0.51722536260948 1103 3 0.4959814684332997 1103 4 0.3745888429649469 1103 5 0.3785629923573026 1103 6 0.3700001115391558 1103 7 0.4490155068791992 1103 8 0.49787487263392194 1103 9 0.5629910840987762 1103 10-11 0.47893820962764266 1103 12-13 0.6914912996147038 1103 14-15 0.7230477542814668 1103 16-17 0.8342080818345394 1103 18-19 0.8582277581085478 1103 20-21 0.789095959227545 1103 22-23 0.6832732745365249 1103 24-25 0.8447761157250611 1103 26-27 0.9116078272578605 1103 28-29 0.9521102631833642 1103 30-31 0.996542354354311 1103 32-33 1.0347025636459293 1103 34-35 1.007900423188353 1103 36-37 1.1601024949867096 1103 38-39 1.2200352593044954 1103 40-41 1.229418194714654 1103 42-43 1.3188113889644129 1103 44-45 1.3790540506979951 1103 46-47 1.5079822032695134 1103 48-49 1.5053282422103678 1103 50-51 1.605068932396584 1103 52-53 1.6826350995194872 1103 54-55 1.7926828945005298 1103 56-57 1.7137929963351368 1103 58-59 2.0100551666181445 1103 60-61 2.273489650049555 1103 62-63 2.2794543068858815 1103 64-65 2.357470996761055 1103 66-67 2.338803768342256 1103 68-69 2.621396669005186 1103 70-71 2.6207590142697548 1103 72-73 2.8065765601450536 1103 74-75 3.1920029436373305 1103 76-77 3.238097474725592 1103 78-79 3.2362870549386464 1103 80-81 3.6980571696408084 1103 82-83 3.987690614186686 1103 84-85 4.102983796261196 1103 86-87 4.270791111904465 1103 88-89 4.538207767061792 1103 90-91 5.011014572169685 1103 92-93 5.067837229997874 1103 94-95 5.192044486856744 1103 96-97 5.394146614314867 1103 98-99 5.4734917942433725 1103 100-101 5.421177486045005 1104 1 0.41109299019251466 1104 2 0.3853785629113631 1104 3 0.3041235054137701 1104 4 0.25436200517226837 1104 5 0.27226133111132356 1104 6 0.3175710222146151 1104 7 0.2979146731116131 1104 8 0.391194399686448 1104 9 0.5005212946969735 1104 10-11 0.4396195091550581 1104 12-13 0.5063144272314588 1104 14-15 0.597355886660047 1104 16-17 0.6247299278031662 1104 18-19 0.7844803748629587 1104 20-21 0.703981754181811 1104 22-23 0.6724776464256195 1104 24-25 0.6597839884497247 1104 26-27 0.6570016656435129 1104 28-29 0.6867360448404796 1104 30-31 0.8457143144268997 1104 32-33 0.8072823465788446 1104 34-35 0.9021479996020716 1104 36-37 1.009610758539921 1104 38-39 1.079391728640175 1104 40-41 1.254389163340555 1104 42-43 1.1939970325287632 1104 44-45 1.0718191739662188 1104 46-47 1.0659743103295867 1104 48-49 1.164001603875036 1104 50-51 1.3858442104688535 1104 52-53 1.2865487196700727 1104 54-55 1.436083338787249 1104 56-57 1.5396555145288815 1104 58-59 1.693629192691354 1104 60-61 1.6888245047691441 1104 62-63 1.8465285732961902 1104 64-65 1.8440090868223287 1104 66-67 1.8861508510541576 1104 68-69 2.256213260351256 1104 70-71 2.214268733322424 1104 72-73 2.2882218809838477 1104 74-75 2.4056179842475593 1104 76-77 2.5743693970630197 1104 78-79 2.827940057580104 1104 80-81 3.165433317418927 1104 82-83 3.4318955139189455 1104 84-85 3.615696168904993 1104 86-87 3.6628799220785844 1104 88-89 3.9910458786860197 1104 90-91 4.2418866589246385 1104 92-93 4.284980677554966 1104 94-95 4.4217262311201 1104 96-97 4.59776054308524 1104 98-99 4.823128312455083 1104 100-101 4.874740483565267 1105 1 0.5744510591395482 1105 2 0.6227975300459434 1105 3 0.591525512181164 1105 4 0.4562827144087649 1105 5 0.4802442199853232 1105 6 0.44799059238447114 1105 7 0.4799253769877865 1105 8 0.49220699642335575 1105 9 0.5299902467556663 1105 10-11 0.5602726298626237 1105 12-13 0.6406751888985198 1105 14-15 0.7459516310735879 1105 16-17 0.8490827118160311 1105 18-19 0.8979413576429565 1105 20-21 0.8967477388044145 1105 22-23 0.8196588752775043 1105 24-25 0.7888064221995919 1105 26-27 0.8966131266639792 1105 28-29 1.0126254171812619 1105 30-31 1.058297441164818 1105 32-33 1.110344006935975 1105 34-35 1.1579717918564185 1105 36-37 1.274269436385076 1105 38-39 1.2293166633113586 1105 40-41 1.2428132939646943 1105 42-43 1.3845086493333483 1105 44-45 1.4115167978397167 1105 46-47 1.504889977521124 1105 48-49 1.420105870349623 1105 50-51 1.2828566731979194 1105 52-53 1.6258217062022808 1105 54-55 1.6735390533840544 1105 56-57 1.7388774315110282 1105 58-59 1.832922489786732 1105 60-61 2.074299730069825 1105 62-63 2.219489141489433 1105 64-65 2.4604771845810482 1105 66-67 2.4356554408226607 1105 68-69 2.533303468565933 1105 70-71 2.6933039396944984 1105 72-73 2.827542084340749 1105 74-75 2.86782201537088 1105 76-77 3.033471670170993 1105 78-79 3.2717558051763405 1105 80-81 3.641460990037775 1105 82-83 4.070803329088633 1105 84-85 4.207469134498478 1105 86-87 4.410566507056306 1105 88-89 4.962159441954473 1105 90-91 5.0913208945774215 1105 92-93 5.143845296135158 1105 94-95 5.370799692542594 1105 96-97 5.49310691147279 1105 98-99 5.799364848854964 1105 100-101 5.587714012829229 1106 1 0.28609299019251466 1106 2 0.37087497439461714 1106 3 0.20665540334040244 1106 4 0.21041264313080177 1106 5 0.26411739171738446 1106 6 0.10709454693551379 1106 7 0.32584529512117655 1106 8 0.190676058378628 1106 9 0.20260462803030777 1106 10-11 0.10217931776749367 1106 12-13 0.038097720692384485 1106 14-15 0.5351997861815718 1106 16-17 0.2589703584251737 1106 18-19 0.47007148331591253 1106 20-21 0.31696520713236964 1106 22-23 0.36272884259786764 1106 24-25 0.44454774443058653 1106 26-27 0.4788318091841859 1106 28-29 0.3696557019377664 1106 30-31 0.6082840911414138 1106 32-33 0.7459785188276555 1106 34-35 0.5483651048652334 1106 36-37 0.6285352003262048 1106 38-39 0.6200665691505449 1106 40-41 0.7521413563230155 1106 42-43 0.3535564424171227 1106 44-45 0.4853409044287389 1106 46-47 0.720330370935649 1106 48-49 0.9133935496485606 1106 50-51 0.24593192976710299 1106 52-53 0.8255489190321086 1106 54-55 0.7853207789786296 1106 56-57 0.6486268063949083 1106 58-59 0.07125578758768825 1106 60-61 1.1995103101917906 1106 62-63 1.3933387806327175 1106 64-65 1.5098284648127596 1106 66-67 1.587406831915402 1106 68-69 1.009916410271515 1106 70-71 1.3712713250289639 1106 72-73 1.8513099990061797 1106 74-75 2.039205504183766 1106 76-77 1.9278482646866202 1106 78-79 2.565131046415832 1106 80-81 2.9663105104013816 1106 82-83 3.0390775314628087 1106 84-85 3.1660051800692663 1106 86-87 3.3544518917755575 1106 88-89 3.4778182072346624 1106 90-91 4.002712017776311 1106 92-93 4.103720709452897 1106 94-95 4.216433168919146 1106 96-97 4.5355296818412185 1106 98-99 4.704991350732595 1106 100-101 4.122612293772603 1107 1 0.42758625824637164 1107 2 0.33638070489422844 1107 3 0.32965900112980506 1107 4 0.23946757432771903 1107 5 0.3495945624454677 1107 6 0.37111294387924687 1107 7 0.30665090322177235 1107 8 0.2429385857329578 1107 9 0.2433839017961148 1107 10-11 0.3293073916520015 1107 12-13 0.46853520446524044 1107 14-15 0.48653504822674876 1107 16-17 0.6749823757835784 1107 18-19 0.6515206135410594 1107 20-21 0.7260403527130279 1107 22-23 0.6190963734758057 1107 24-25 0.6541727583395627 1107 26-27 0.7463376509556312 1107 28-29 0.690954986088947 1107 30-31 0.8068449753816154 1107 32-33 0.8251985032495952 1107 34-35 0.8815352395041529 1107 36-37 0.8459594427504484 1107 38-39 0.9806883932668597 1107 40-41 0.994226219644105 1107 42-43 1.1180254903011004 1107 44-45 1.176543011175525 1107 46-47 1.2220560116759813 1107 48-49 1.3054413223572823 1107 50-51 1.2376189962705624 1107 52-53 1.411743793109487 1107 54-55 1.5062065028019376 1107 56-57 1.7057815855203202 1107 58-59 1.7615797740867052 1107 60-61 1.6992590212930168 1107 62-63 1.8645824288653472 1107 64-65 2.032725427091602 1107 66-67 2.081667986917072 1107 68-69 2.2322727768751243 1107 70-71 2.3488426317312374 1107 72-73 2.6495361404697775 1107 74-75 2.731295003831402 1107 76-77 2.915916214688476 1107 78-79 2.9751937295507282 1107 80-81 3.119197087308766 1107 82-83 3.4012154343595853 1107 84-85 3.6436107955879784 1107 86-87 3.9872335022499463 1107 88-89 4.156582904389815 1107 90-91 4.420482130160867 1107 92-93 4.629992917457049 1107 94-95 4.710733269063795 1107 96-97 4.824374680172138 1107 98-99 4.952515552357163 1107 100-101 4.769381701435522 1108 1 0.27382326314926075 1108 2 0.38483423683701545 1108 3 0.25602507009401165 1108 4 0.3154075619820773 1108 5 0.2537190667148508 1108 6 0.26723639151364864 1108 7 0.2926760123399319 1108 8 0.310598864826062 1108 9 0.23125803097466502 1108 10-11 0.38370531571793265 1108 12-13 0.3871230380682036 1108 14-15 0.2346325218920029 1108 16-17 0.4500861159724252 1108 18-19 0.41385658992026464 1108 20-21 0.4692793746292665 1108 22-23 0.42141917473254153 1108 24-25 0.5017710284917385 1108 26-27 0.44519189658279146 1108 28-29 0.43618226287663475 1108 30-31 0.4821771062366267 1108 32-33 0.44388790762175745 1108 34-35 0.588741067112224 1108 36-37 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2.3666133731272474 2311 86-87 2.689351022357137 2311 88-89 2.7477375294703634 2311 90-91 2.8924309339576233 2311 92-93 3.16476370057212 2311 94-95 3.15815325794901 2311 96-97 3.2618521352271515 2311 98-99 3.513180632496134 2311 100-101 3.5840953252307113 2312 1 -0.27148207545424796 2312 2 -0.24145883291007664 2312 3 -0.19906892522899966 2312 4 -0.19566725045051925 2312 5 -0.1904463124552933 2312 6 -0.16879086270375154 2312 7 -0.14122364176451896 2312 8 -0.16306407730864692 2312 9 -0.08074583054563789 2312 10-11 -0.16652845294107266 2312 12-13 -0.15873687714118745 2312 14-15 0.08506460055432541 2312 16-17 -0.033055635617323276 2312 18-19 -0.04877220819446393 2312 20-21 0.0017429854867572203 2312 22-23 -0.010574071807049279 2312 24-25 -0.17117880696071097 2312 26-27 -0.0955057552949512 2312 28-29 -0.11620200459438479 2312 30-31 0.04494724626843549 2312 32-33 0.1959711238365287 2312 34-35 0.06310280323261708 2312 36-37 0.050541872251535835 2312 38-39 0.1602888772872788 2312 40-41 0.21054889904097962 2312 42-43 0.3216678568237725 2312 44-45 0.1111619214259747 2312 46-47 0.18964068206151552 2312 48-49 0.25236286086784077 2312 50-51 0.4312318117932037 2312 52-53 0.4351233999988793 2312 54-55 0.6106362986000065 2312 56-57 0.7185372361866058 2312 58-59 0.7134427416124609 2312 60-61 0.8430787004535816 2312 62-63 0.7868827803974554 2312 64-65 0.9845095720375348 2312 66-67 1.1590101957282073 2312 68-69 1.2336389796114489 2312 70-71 1.37311285080159 2312 72-73 1.3478869397805155 2312 74-75 1.3561884688919257 2312 76-77 1.5493586937863846 2312 78-79 1.8148556586761195 2312 80-81 1.8769671683151508 2312 82-83 2.1332787340354002 2312 84-85 2.335771948103531 2312 86-87 2.506338436178371 2312 88-89 2.403309693048392 2312 90-91 2.4183351690285306 2312 92-93 2.7091015817677793 2312 94-95 2.923740587781822 2312 96-97 3.094343343633245 2312 98-99 3.2186033238719105 2312 100-101 3.1501661171992392 2313 1 -0.37136915832947537 2313 2 -0.4522327037959748 2313 3 -0.5076263975311583 2313 4 -0.3543469543973572 2313 5 -0.25448877980876006 2313 6 -0.3662592561947875 2313 7 -0.3514440701638577 2313 8 -0.30224329095744196 2313 9 -0.5198945862857798 2313 10-11 -0.3446409311976666 2313 12-13 -0.37928727955129915 2313 14-15 -0.34792914328837554 2313 16-17 -0.3932228341068722 2313 18-19 -0.2380542530001648 2313 20-21 -0.21408972687858352 2313 22-23 -0.3207680138261608 2313 24-25 -0.2681285302680365 2313 26-27 -0.2476230085287483 2313 28-29 -0.16309046921863768 2313 30-31 -0.14763845302915257 2313 32-33 -0.21629995612205732 2313 34-35 -0.1647553543439102 2313 36-37 -0.06513117895661225 2313 38-39 -0.002605075474960472 2313 40-41 0.01570457152645588 2313 42-43 0.07922490397238846 2313 44-45 0.11645705456074751 2313 46-47 0.05201536462497103 2313 48-49 0.2284832604664615 2313 50-51 0.27852625805356723 2313 52-53 0.36633024177089624 2313 54-55 0.3155511650309393 2313 56-57 0.3340887129094483 2313 58-59 0.39408171196371455 2313 60-61 0.4707320528807344 2313 62-63 0.5394809885809764 2313 64-65 0.6088124116517903 2313 66-67 0.7673256333734955 2313 68-69 0.8407418153398893 2313 70-71 1.0293249212765012 2313 72-73 1.1497565296444918 2313 74-75 1.2146023375834822 2313 76-77 1.341111743577354 2313 78-79 1.5161738632481239 2313 80-81 1.7083274996606228 2313 82-83 1.7756085983291285 2313 84-85 1.9854922839291191 2313 86-87 1.978353446841492 2313 88-89 2.0761136092611423 2313 90-91 2.4324466666890423 2313 92-93 2.3934327878095303 2313 94-95 2.5725210428332623 2313 96-97 2.771991381641058 2313 98-99 2.956825313453365 2313 100-101 2.863056612731702 2314 1 -0.2932258309912186 2314 2 -0.4417226374819414 2314 3 -0.4780027202437829 2314 4 -0.3553229086561487 2314 5 -0.28204298019886664 2314 6 -0.3097794065948207 2314 7 -0.3308377508907938 2314 8 -0.2484247246682898 2314 9 -0.31399421209023615 2314 10-11 -0.24383650373653865 2314 12-13 -0.2853675743799897 2314 14-15 -0.3965753160548431 2314 16-17 -0.4236919100686265 2314 18-19 -0.25843035698091654 2314 20-21 -0.2209524392740576 2314 22-23 -0.24666685143032652 2314 24-25 -0.22306696271516557 2314 26-27 -0.23832888634621696 2314 28-29 -0.25792611409213606 2314 30-31 -0.16077740299586907 2314 32-33 -0.054939063849147374 2314 34-35 -0.1365541860969941 2314 36-37 0.06449862282310193 2314 38-39 0.17684131963244454 2314 40-41 0.2031938058172713 2314 42-43 0.38567775385725866 2314 44-45 0.3688611228641463 2314 46-47 0.25641674197954245 2314 48-49 0.4270265198884715 2314 50-51 0.36401384966804784 2314 52-53 0.44024062896458105 2314 54-55 0.4967941006750962 2314 56-57 0.5613151709350817 2314 58-59 0.7984677234044852 2314 60-61 0.7393215959662598 2314 62-63 0.883817241255656 2314 64-65 0.8537428221076091 2314 66-67 1.1351952478531082 2314 68-69 1.3496064028143167 2314 70-71 1.428771351927839 2314 72-73 1.3681181951345636 2314 74-75 1.4040547587538832 2314 76-77 1.634150588169998 2314 78-79 1.890510217086291 2314 80-81 2.1781719621625975 2314 82-83 2.387627682137886 2314 84-85 2.5697836856340253 2314 86-87 2.6187196340661316 2314 88-89 2.696155914738789 2314 90-91 2.838620401038092 2314 92-93 2.9297038633086636 2314 94-95 3.082142442575048 2314 96-97 3.248899994919288 2314 98-99 3.4264303329122967 2314 100-101 3.250859877632946 2315 1 -0.49300221974243286 2315 2 -0.4243510887202717 2315 3 -0.4250194170097572 2315 4 -0.42270206396379706 2315 5 -0.3407981146173995 2315 6 -0.20304379688113272 2315 7 -0.3335950575480311 2315 8 -0.46646782277471033 2315 9 -0.6200196036418291 2315 10-11 -0.47010308640432186 2315 12-13 -0.456318798132763 2315 14-15 -0.5830559527571921 2315 16-17 -0.4487348724763862 2315 18-19 -0.46846855933412 2315 20-21 -0.3838040167444774 2315 22-23 -0.5014357184472331 2315 24-25 -0.47975336841280836 2315 26-27 -0.47713964396349695 2315 28-29 -0.5190729506436327 2315 30-31 -0.48223613178566893 2315 32-33 -0.4627548826023826 2315 34-35 -0.5856612915650672 2315 36-37 -0.3283255419277893 2315 38-39 -0.2883850373939154 2315 40-41 -0.22311221427354866 2315 42-43 -0.14331782957542316 2315 44-45 -0.13340719155176828 2315 46-47 -0.0630715070995862 2315 48-49 0.07465056092035383 2315 50-51 0.19010607811038227 2315 52-53 0.23485845436174912 2315 54-55 0.2746721579367559 2315 56-57 0.5905022431993885 2315 58-59 0.7180507627682289 2315 60-61 0.7300628790500951 2315 62-63 0.7571583781453946 2315 64-65 0.8070813006817978 2315 66-67 1.0142803871006087 2315 68-69 1.1715627375299036 2315 70-71 1.1803322178125057 2315 72-73 1.264810169247923 2315 74-75 1.4819756061496072 2315 76-77 1.5965694803918709 2315 78-79 1.829209142575536 2315 80-81 1.7829095283753276 2315 82-83 2.0162772339877577 2315 84-85 2.2170405186711335 2315 86-87 2.318692613600547 2315 88-89 2.6907149152969545 2315 90-91 2.6914249901971488 2315 92-93 2.7579525607870536 2315 94-95 2.9077844403561635 2315 96-97 3.037487909893475 2315 98-99 3.2586107797955464 2315 100-101 3.1623955491531373 2316 1 -0.7590752695058356 2316 2 -0.7761376716540056 2316 3 -0.7030063369239521 2316 4 -0.4499084991282274 2316 5 -0.5201927068479577 2316 6 -0.5256079135308269 2316 7 -0.42702533181106617 2316 8 -0.5508565089569473 2316 9 -0.502551871949187 2316 10-11 -0.43988952258251146 2316 12-13 -0.5123212659082483 2316 14-15 -0.9743886112273046 2316 16-17 -0.7616668293859945 2316 18-19 -0.6946599264456452 2316 20-21 -0.5370163775954069 2316 22-23 -0.7694445548296542 2316 24-25 -0.8827065849733131 2316 26-27 -0.6501035633291536 2316 28-29 -0.6235876807341754 2316 30-31 -0.6062967171465701 2316 32-33 -0.41891909330300336 2316 34-35 -0.5190057775239083 2316 36-37 -0.4596718900632695 2316 38-39 -0.46530439031074877 2316 40-41 -0.5298785805026895 2316 42-43 -0.31098315713088454 2316 44-45 -0.41799631980522634 2316 46-47 -0.22542152385837966 2316 48-49 -0.016278732097397608 2316 50-51 0.06102402477468871 2316 52-53 0.10903136211992148 2316 54-55 0.07805797163788242 2316 56-57 0.3577412100615547 2316 58-59 0.4471953530747754 2316 60-61 0.3326650101635735 2316 62-63 0.5310296740605729 2316 64-65 0.6852517711832711 2316 66-67 0.8237298189337174 2316 68-69 1.0210044826099427 2316 70-71 0.8706998723058952 2316 72-73 1.1669149967623866 2316 74-75 1.4344598485048543 2316 76-77 1.440607808270304 2316 78-79 1.625258140882675 2316 80-81 1.83313989875926 2316 82-83 2.0869108716331866 2316 84-85 2.0845642788720404 2316 86-87 2.525622679937772 2316 88-89 2.5311090017118225 2316 90-91 2.743872649817689 2316 92-93 2.737856339039155 2316 94-95 2.923110476515351 2316 96-97 2.752775943531013 2316 98-99 2.916136108396614 2316 100-101 2.858429215088149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7986.0 3 2656.0 4 2906.0 5 3460.0 6 4131.0 7 5505.0 8 7051.0 9 9301.0 10 12040.0 11 15665.0 12 19894.0 13 24247.0 14 28054.0 15 33527.0 16 39251.0 17 45877.0 18 43305.0 19 40566.0 20 42722.0 21 46107.0 22 51706.0 23 57425.0 24 65165.0 25 72772.0 26 81943.0 27 92845.0 28 105472.0 29 119087.0 30 135729.0 31 156365.0 32 180678.0 33 212128.0 34 253126.0 35 304932.0 36 355009.0 37 357303.0 38 189776.0 39 14699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21524211162344 18.042200523993245 14.380655338243088 23.36190202614023 2 15.759725146846948 19.63351472266634 39.76859508214384 24.838165048342876 3 19.47858926045282 23.66437973412634 29.318440176573997 27.538590828846843 4 13.097577211112869 15.921766321530193 35.9259220380177 35.054734429339234 5 13.920331118404658 37.35787695396512 33.55229414796267 15.169497779667553 6 33.26620392456927 35.29530918589072 16.725783158423244 14.712703731116767 7 29.542020975450438 32.720998849789126 19.545527791206165 18.191452383554267 8 27.508897643069997 33.8599425006506 19.237030000855114 19.394129855424296 9 27.17115294787405 16.462582009739137 19.43137238557536 36.93489265681145 10-11 25.456856533164572 26.127549228816076 26.602769856490717 21.812824381528632 12-13 26.702920854096835 23.506154804587965 26.885431787156193 22.905492554159007 14-15 23.125563459617744 24.840471761846487 25.35905911871173 26.674905659824045 16-17 22.575041759532077 28.12785956141649 26.362823853408955 22.934274825642476 18-19 22.754099058997557 26.21832713694975 28.852525864911016 22.175047939141677 20-21 23.09264559645606 25.33676720157747 29.25803315543451 22.31255404653196 22-23 22.941079228092153 25.343123234489166 29.154365362284633 22.561432175134044 24-25 22.704673209712954 25.576623129210112 29.224114387677425 22.494589273399512 26-27 21.893101998247573 26.077374744437837 28.973709901208363 23.05581335610623 28-29 22.367132547617167 26.52602458409073 28.753541032759028 22.353301835533077 30-31 22.208346884941328 25.977409887531046 28.539848519392603 23.274394708135024 32-33 21.784437123301036 25.966961007913998 28.647273319988987 23.601328548795983 34-35 23.043137610821493 26.154952982575725 28.340820990506995 22.46108841609579 36-37 23.243340526645532 25.32863445044479 28.336566748004966 23.091458274904713 38-39 22.40780207024882 25.849558708982823 28.29593159113396 23.446707629634396 40-41 23.077469174147257 25.68675732632461 28.49153760024817 22.744235899279968 42-43 22.699631543483083 25.75834228212157 28.21066008258093 23.331366091814413 44-45 22.51636753522959 26.15419941896909 28.5523947849806 22.77703826082072 46-47 23.22893004674744 26.578075294384178 27.45109235547932 22.741902303389054 48-49 22.755137330623608 26.636802912649678 27.314347055875942 23.293712700850772 50-51 23.18921716555248 26.756804468384075 27.330675667793248 22.723302698270192 52-53 23.52694825610777 26.152326797032625 27.529232039318106 22.7914929075415 54-55 22.64487841868132 26.755189139783838 27.714093439622438 22.88583900191241 56-57 22.807851633416583 26.862949786549823 27.394441885459848 22.934756694573743 58-59 22.75056857093565 26.149463865924353 27.424840462189763 23.675127100950235 60-61 22.075882344590138 26.65285876622675 27.88572465552776 23.385534233655353 62-63 22.48174767990228 26.576155774528875 28.08582795338541 22.856268592183437 64-65 22.17976836965427 26.6675040843401 27.730288960021383 23.422438585984253 66-67 22.019895651830414 26.658562493789166 27.76973841359427 23.55180344078615 68-69 22.87234058969583 26.150774116590465 27.922577538968163 23.054307754745544 70-71 22.60846430038609 26.35486430754672 27.33046969740461 23.70620169466258 72-73 22.47531582472461 26.59687092005074 27.354047772637063 23.573765482587586 74-75 22.476046414585607 26.995391380783307 27.313568821882363 23.214993382748727 76-77 22.747367654338973 26.38266818432654 27.441177305717517 23.428786855616966 78-79 22.821196300112927 26.743954938681387 27.336185620239696 23.098663140965993 80-81 23.01159181753693 26.677043683000306 27.045449881001744 23.265914618461018 82-83 22.795731647094843 26.757976464429984 27.240502562937653 23.205789325537527 84-85 22.690127783152867 26.903962805396485 27.233628217532264 23.17228119391838 86-87 23.10700998485815 26.889495029327794 26.676925825908356 23.32656915990571 88-89 22.825943694847023 26.915316326407773 26.62606463051499 23.63267534823021 90-91 22.678550646703076 26.851829842956988 26.79899649043304 23.67062301990689 92-93 22.8458578296415 26.332569328358762 27.104835762593428 23.71673707940631 94-95 22.594300084469147 26.09202954213032 27.39112269880229 23.922547674598245 96-97 22.988662384188114 25.602782031739395 27.339761629648585 24.068793954423906 98-99 22.604611053119893 25.92532177925983 26.961108089806213 24.50895907781407 100-101 22.978503732235506 25.338267632271773 27.46171222521065 24.221516410282078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 682.0 1 491.5 2 411.0 3 518.5 4 463.0 5 463.5 6 794.5 7 1730.0 8 2845.0 9 3232.0 10 2840.5 11 2495.5 12 2485.5 13 2641.5 14 2941.5 15 3197.0 16 3420.5 17 3666.0 18 4154.5 19 4731.0 20 5263.5 21 6208.5 22 7474.5 23 9048.5 24 11010.5 25 13622.0 26 17206.5 27 21351.5 28 24956.0 29 28521.5 30 32982.0 31 41411.0 32 54486.0 33 57815.5 34 54143.0 35 56557.0 36 62945.0 37 69742.0 38 74961.0 39 79163.5 40 83710.5 41 90132.0 42 94477.0 43 98655.5 44 103560.0 45 110586.0 46 116028.0 47 120196.0 48 121547.0 49 118906.0 50 118628.0 51 117038.5 52 114736.5 53 116338.5 54 120800.5 55 116207.5 56 106249.0 57 98392.0 58 92917.5 59 83428.0 60 67479.0 61 56498.5 62 49349.0 63 37976.0 64 27202.0 65 21035.5 66 15839.0 67 11999.5 68 9447.5 69 7483.0 70 5834.5 71 4111.0 72 2953.0 73 2276.0 74 1686.5 75 1222.0 76 897.0 77 632.5 78 420.0 79 278.5 80 186.5 81 115.0 82 66.5 83 42.0 84 29.5 85 24.5 86 20.5 87 10.5 88 5.0 89 4.0 90 2.5 91 3.0 92 2.5 93 2.5 94 3.0 95 1.5 96 1.0 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30351088179863606 2 0.03561276640524921 3 0.044099344188129226 4 0.04625956398740777 5 0.05962206646008794 6 0.05832593458052081 7 0.031014584261070587 8 0.03302050264611495 9 0.04055041166074304 10-11 0.03312851363607888 12-13 0.035581906122402376 14-15 0.02893151516890913 16-17 0.02257429690246083 18-19 0.027373070885143888 20-21 0.04024180883227467 22-23 0.033529697313087754 24-25 0.05263221239527948 26-27 0.045457196633390026 28-29 0.03835933157861765 30-31 0.03859078369996893 32-33 0.03354512745451117 34-35 0.033329105474583315 36-37 0.03269646967622317 38-39 0.02022891540610126 40-41 0.019704290597705044 42-43 0.020984992335848756 44-45 0.009504967116825613 46-47 0.017112026838570788 48-49 0.014643204210823874 50-51 0.006912703357691355 52-53 0.005354259073926116 54-55 0.007853941984519865 56-57 0.005971464730862844 58-59 0.008332276368645829 60-61 0.007313887034700228 62-63 0.004258719032863424 64-65 0.00899577244985281 66-67 0.0032403296989178226 68-69 0.008208835237258483 70-71 0.014627774069400456 72-73 0.01484379604932831 74-75 0.01218981172450038 76-77 0.010338194753690196 78-79 0.006295497700754627 80-81 0.007329317176123646 82-83 0.011696047198950997 84-85 0.004814204124106479 86-87 0.001681885415152584 88-89 0.020738110073074065 90-91 0.03024307718989968 92-93 0.014411752089472601 94-95 0.023654406802100104 96-97 0.01914880550646199 98-99 0.014103149261004236 100-101 0.10730120345845018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3240411.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.786542958394804 #Duplication Level Percentage of deduplicated Percentage of total 1 71.05466778064404 30.401835953909977 2 14.171262805370166 12.126786895933463 3 5.681217219310064 7.292389338299471 4 2.8409591250106643 4.862192785812501 5 1.6011228199430936 3.425325515858071 6 0.9966468471714275 2.5585843884509414 7 0.6737099274692553 2.0178003127212243 8 0.47575565081931315 1.6284751673183697 9 0.35674015159753525 1.3737310039180999 >10 1.9064734948256308 14.795855444915135 >50 0.14633564768123034 4.302106065888812 >100 0.08265525074632786 6.845329472434486 >500 0.006918488562047535 2.010275230852695 >1k 0.004952181286125599 4.6129919550967795 >5k 5.097833676893998E-4 1.3934044449789436 >10k+ 7.282619538419999E-5 0.35291602361088414 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11326 0.34952356352326913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8547 0.26376283749191076 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6635 0.20475797668875953 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6581 0.20309152141503037 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6197 0.1912411728018452 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5947 0.18352610209013612 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5597 0.17272500309374336 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5214 0.160905514763405 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4885 0.15075248170679584 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4740 0.14627774069400457 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4698 0.14498160881443742 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4372 0.13492115660636875 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4191 0.12933544541109135 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3870 0.11942929461725689 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3533 0.10902937929787301 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3527 0.108844217600792 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3304 0.10196237452594749 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3272 0.10097484547484871 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3271 0.10094398519200187 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3249 0.10026505896937148 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.172056569367281E-5 0.0 12-13 0.0 0.0 0.0 1.3887127281076383E-4 0.0 14-15 0.0 0.0 0.0 1.5430141423418203E-4 0.0 16-17 0.0 0.0 0.0 1.5430141423418203E-4 0.0 18-19 0.0 0.0 0.0 1.5430141423418203E-4 0.0 20-21 0.0 0.0 0.0 1.5430141423418203E-4 0.0 22-23 0.0 0.0 0.0 4.474741012791279E-4 0.0 24-25 0.0 0.0 0.0 9.720989096753468E-4 0.0 26-27 0.0 0.0 0.0 0.002067638950738039 0.0 28-29 0.0 0.0 0.0 0.005199957659691935 0.0 30-31 0.0 0.0 0.0 0.01647939104021064 0.0 32-33 0.0 0.0 0.0 0.04047326095362594 0.0 34-35 0.0 0.0 0.0 0.07641006032876693 0.0 36-37 0.0 0.0 0.0 0.13708137640564733 0.0 38-39 0.0 0.0 0.0 0.22605157185307667 0.0 40-41 0.0 0.0 0.0 0.32394038904324174 0.0 42-43 0.0 0.0 0.0 0.422832165425929 0.0 44-45 0.0 0.0 0.0 0.5482329247740487 0.0 46-47 0.0 0.0 0.0 0.6970412086614939 0.0 48-49 0.0 0.0 0.0 0.8556784926356564 0.0 50-51 0.0 0.0 0.0 1.0311809211856149 0.0 52-53 0.0 0.0 0.0 1.1958051000320638 0.0 54-55 0.0 0.0 0.0 1.3716315615519141 0.0 56-57 0.0 0.0 0.0 1.5776548098373941 0.0 58-59 0.0 0.0 0.0 1.8010369672242192 0.0 60-61 0.0 0.0 0.0 2.0496782661211803 0.0 62-63 0.0 0.0 0.0 2.292255519438738 0.0 64-65 0.0 0.0 0.0 2.5389680506577714 0.0 66-67 0.0 0.0 0.0 2.830659444126069 0.0 68-69 0.0 0.0 0.0 3.151575525450321 0.0 70-71 0.0 0.0 0.0 3.4809010338503357 0.0 72-73 0.0 0.0 0.0 3.8320910526473337 0.0 74-75 0.0 0.0 0.0 4.183049619322981 0.0 76-77 0.0 0.0 0.0 4.553758767020604 0.0 78-79 0.0 0.0 0.0 4.975418241698352 1.5430141423418203E-5 80-81 0.0 0.0 0.0 5.4234015376444535 3.0860282846836406E-5 82-83 0.0 0.0 0.0 5.887787073923647 3.0860282846836406E-5 84-85 0.0 3.0860282846836406E-5 0.0 6.355875844144462 3.0860282846836406E-5 86-87 0.0 3.0860282846836406E-5 0.0 6.83354673218922 3.0860282846836406E-5 88-89 0.0 3.0860282846836406E-5 0.0 7.336353320612725 3.0860282846836406E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5720 0.0 59.572548 1 GTATCAA 10985 0.0 45.293575 1 TATCAAC 14390 0.0 34.511913 2 ATCAACG 14590 0.0 33.84391 3 TCAACGC 14880 0.0 33.21626 4 CAACGCA 15035 0.0 32.87382 5 AACGCAG 15510 0.0 31.893257 6 TGGTATC 2290 0.0 30.921774 2 CGCAGAG 17945 0.0 27.136457 8 ACGCAGA 18025 0.0 27.095087 7 TACATGG 13675 0.0 25.577852 2 GTACATG 14220 0.0 25.16961 1 TAAGGTG 2355 0.0 25.023674 5 GTAAGGT 2470 0.0 24.435827 4 GCAGAGT 19915 0.0 23.878464 9 GTGGTAT 3150 0.0 23.75337 1 ACATGGG 14165 0.0 23.72046 3 AGGTAAG 2310 0.0 23.247702 2 TAAGGTA 1310 0.0 23.218222 4 GTGTAAG 1400 0.0 23.148188 1 >>END_MODULE