FastQCFastQC Report
Thu 26 May 2016
SRR938613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240411
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106060.32730415987354694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.2731752237601959No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66690.20580722630555198No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA66550.20537518234569627No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64140.1979378541796087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.189389555831035No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC58370.1801314709769841No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56630.17476178176163457No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53140.16399154304808863No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48930.15099936396957053No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA44490.13729739838557517No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43090.13297695878701807No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42000.1296131879567129No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA39660.12239188177055318No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38550.11896639037455434No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36290.11199196645116931No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG35410.1092762615606477No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35220.10868991618655781No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34350.10600507157888304No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA32880.10146861000039809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.059.6527561
GTATCAA109750.044.644391
TGGTATC22050.033.0078352
ATCAACG147700.032.8632933
TATCAAC148250.032.8578762
TCAACGC149250.032.4901474
CAACGCA151550.032.028435
AACGCAG157350.030.8485166
TAAGGTG25350.028.6932225
AGGTAAG24000.026.95642
GTAAGGT26400.026.6516194
ACGCAGA182400.026.5077657
GGTAAGG26500.026.3716473
CGCAGAG182150.026.2833968
AAGGTAA24300.025.8405571
GTGGTAT32700.024.4396931
TACATGG134050.023.066452
GCAGAGT206700.022.8629749
GTACATG139150.022.6312751
TAAGGTA12900.022.48054
Produced by FastQC (version 0.11.5)